Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| preventive effect of fermented brown rice and rice bran on diethylnitrosoamine and phenobarbital-induced hepatocarcinogenesis in male f344 rats. | epidemiological and preclinical studies have suggested that nutrition plays an important role in the etiology of cancer. our group previously demonstrated that rice germ or fermented brown rice has a preventive effect on colorectal carcinogenesis. the experiment described here was examined for the potential anticancer properties of brown rice fermented by aspergillus oryzae (fbra) in male f344 rats using inhibition of diethylnitrosoamine (den) and phenobarbital (pb)-induced hepatocarcinogenesis ... | 2003 | 12792738 |
| substrate aggregation due to aerial hyphae during discontinuously mixed solid-state fermentation with aspergillus oryzae: experiments and modeling. | solid-state fermentation (ssf) is prone to process failure due to channeling caused by evaporative cooling and the formation of an interparticle mycelium network. mixing is needed to break the mycelium network and to avoid such failure. this study presents the first attempt to quantify and predict the effect of mycelium bonds on particle mixing and vice versa. we developed a novel experimental set-up to measure the tensile strength of hyphal bonds in ssf: aspergillus oryzae was cultivated betwee ... | 2003 | 12827692 |
| construction of an efficient expression system for aspergillus isopullulanase in pichia pastoris, and a simple purification method. | aspergillus niger atcc 9642 isopullulanase (ipu) was heterologously expressed by pichia pastoris gs115 under three different signal sequences of saccharomyces cerevisiae acid phosphatase, s. cerevisiae alpha-factor prepro peptide, and a. niger isopullulanase. one-step purification using lectin con a affinity chromatography yielded recombinant ipu (ipu-pp) with high purity. ipu-pp had a higher carbohydrate content than native ipu and ipu-ao expressed in a. oryzae m-2-3. ipu-pp hydrolyzed various ... | 2003 | 12834298 |
| gene cloning and functional analysis of a second delta 6-fatty acid desaturase from an arachidonic acid-producing mortierella fungus. | we demonstrated that mortierella alpina 1s-4 has two delta 6-desaturases, which are involved in the desaturation of linoleic acid to gamma-linolenic acid. for one of the two delta 6-desaturases, designated as delta 6i, gene cloning and its heterologous expression in a fungus, aspergillus oryzae, has previously been reported. in addition, we indicated in this paper that there is an isozyme of the two delta 6-desaturases, designated as delta 6ii, in m. alpina 1s-4. the predicted amino acid sequenc ... | 2003 | 12784608 |
| a novel heterofunctional epoxy-amino sepabeads for a new enzyme immobilization protocol: immobilization-stabilization of beta-galactosidase from aspergillus oryzae. | the properties of a new and commercially available amino-epoxy support (amino-epoxy-sepabeads) have been compared to conventional epoxy supports to immobilize enzymes, using the beta-galactosidase from aspergillus oryzae as a model enzyme. the new support has a layer of epoxy groups over a layer of ethylenediamine that is covalently bound to the support. this support has both a great anionic exchanger strength and a high density of epoxy groups. epoxy supports require the physical adsorption of ... | 2003 | 12790680 |
| in vivo visualization of the distribution of a secretory protein in aspergillus oryzae hyphae using the rnta-egfp fusion protein. | a fusion gene encoding ribonuclease t1-egfp (rnta-egfp) was constructed and expressed to use it as a tool for studies on the secretory pathway in aspergillus oryzae. the successful secretion of the intact rnta-egfp fusion protein was detected by fluorescence measurement and western analysis. with use of the rnta-egfp system, we were able to see high fluorescence at hyphal tips and observe concentrated fluorescence at septa in basal cells during growth at optimal conditions. cold or heat shock du ... | 2003 | 12729022 |
| pulsed feeding during fed-batch aspergillus oryzae fermentation leads to improved oxygen mass transfer. | productivity in many fungal fermentations is detrimentally affected by high broth viscosity and consequent reduced oxygen mass transfer capacity. the goal here was to determine whether pulsed feeding of limiting carbon in a fungal fermentation could lead to reduced viscosity and improved oxygen mass transfer. as a model, an industrially relevant recombinant strain of aspergillus oryzae was grown in carbon-limited, fed-batch mode. maltodextrin was used as a carbon source and was added either cont ... | 2003 | 12790687 |
| study of the inhibition of four alpha amylases by acarbose and its 4iv-alpha-maltohexaosyl and 4iv-alpha-maltododecaosyl analogues. | acarbose analogues, 4iv-maltohexaosyl acarbose (g6-aca) and 4iv-maltododecaosyl acarbose (g12-aca), were prepared by the reaction of cyclomaltodextrin glucanyltransferase with cyclomaltohexaose and acarbose. the inhibition kinetics of acarbose and the two acarbose analogues were studied for four different alpha-amylases: aspergillus oryzae, bacillus amyloliquefaciens, human salivary, and porcine pancreatic alpha-amylases. the three inhibitors showed mixed, noncompetitive inhibition, for all four ... | 2003 | 14499573 |
| protein purification, cdna cloning and characterization of a protease inhibitor from the indian tasar silkworm, antheraea mylitta. | an inhibitor of aspergillus oryzae fungal protease was purified to homogeneity from the hemolymph of fifth instar larvae of antheraea mylitta by ammonium sulfate precipitation, anion exchange and gel filtration (fplc) chromatography, and termed as amfpi-1. the extent of purification was checked by two-dimensional gel electrophoresis, and the molecular weight of purified inhibitor was determined by sds-page as 10.4 kda. fifteen n-terminal amino acid sequences of this protein were determined, and ... | 2003 | 14505696 |
| upregulation of promoter activity of the aspergillus oryzae xylanase gene by site-directed mutagenesis. | in aspergillus oryzae, inductive expression of the xynf1 gene is mediated by a transcriptional activator, aoxlnr. promoter activity of the xynf1 gene, monitored by beta-galactosidase activity, was successfully upregulated by mutating two non-canonical aoxlnr binding sequences to what is thought to be the strongest sequence. transformants carrying three canonical binding sequences in the promoter region produced enzyme 2.8 times more active (33,000 units mg(-1) protein) than that of transformants ... | 2003 | 12882555 |
| solid-phase reducing agents as alternative for reducing disulfide bonds in proteins. | disulfide reduction of kluyveromyces lactis and aspergillus oryzae beta-galactosidases and beta-lactoglobulin was assessed. reduction was performed using one of two thiol-containing agents: dithiothreitol (dtt) or thiopropyl-agarose with a high degree of substitution (1000 micromol of sh groups/g of dried gel). both reductants allowed an increase of three- (for k. lactis beta-galactosidase) and fourfold (for a. oryzae beta-galactosidase) in the initial content of sh groups in the lactases. nearl ... | 2003 | 12909729 |
| authentic and recombinant bilirubin oxidases are in different resting forms. | myrothecium verrucaria bilirubin oxidase expressed in aspergillus oryzae is in a resting form different from that of the authentic bilirubin oxidase, but reaches the resting form of the authentic enzyme after one cycle of reduction and reoxidation with dioxygen as shown by the absorption and electron paramagnetic resonance spectra. | 2003 | 12834300 |
| thermal stability of alpha-amylase from aspergillus oryzae entrapped in polyacrylamide gel. | to determine the suitability as a time-temperature indicator for dielectric pasteurization processes, the thermal stability (50-75 degrees c) of aspergillus oryzae alpha-amylase immobilized in polyacrylamide gel in phosphate buffer, mashed potatoes, and minced shrimp was examined. changing the cross-linking agent concentration from 3.3 to 5.3% and adding 2% salt did not markedly affect the thermal stability of the immobilized alpha-amylase. thermal inactivation was first order, and immobilizatio ... | 2003 | 12926898 |
| isofagomine lactams, synthesis and enzyme inhibition. | the synthesis of isofagomine lactams (2-oxoisofagomines) corresponding to the biologically important hexoses is presented. the d-glucose/d-mannose analogue (3s,4r,5r)-3,4-dihydroxy-5-hydroxymethylpiperidin-2-one (9) was synthesised in 9 steps from d-arabinose, the d-galactose analogue (3s,4s,5r)-3,4-dihydroxy-5-hydroxymethylpiperidin-2-one (10) was synthesised in 11 steps from d-arabinose and the l-fucose analogue (3r,4r,5r)-3,4-dihydroxy-5-methylpiperidin-2-one (11) was synthesised in 12 steps ... | 2003 | 12929423 |
| preferential binding of two compatible solutes to the glycan moieties of peniophora lycii phytase. | regulation of hydration behavior, and the concomitant effects on solubility and other properties, has been suggested as a main function of protein glycosylation. in this work, we have studied the hydration of the heavily glycosylated peniophora lycii phytase in solutions (0.15-1.1 m) of the two compatible solutes glycerol and sorbitol. osmometric measurements showed that glycerol preferentially binds to phytase (i.e., glycerol-glycoprotein interactions are more favorable than water-glycoprotein ... | 2003 | 12939159 |
| transformation of aspergillus aculeatus using the drug resistance gene of aspergillus oryzae and the pyrg gene of aspergillus nidulans. | transformation systems for aspergillus aculeatus has been developed, based on the use of the pyrithiamine resistance gene of aspergillus oryzae and the orotidine-5'-monophosphate decarboxylase gene (pyrg) of aspergillus nidulans. an a. aculeatus mutant which can be transformed effectively by the a. nidulans pyrg gene was isolated as a transformation host. this is the first report of transformation of a. aculeatus. | 2003 | 14730150 |
| thiamine-regulated gene expression of aspergillus oryzae thia requires splicing of the intron containing a riboswitch-like domain in the 5'-utr. | exogenous thiamine regulates aspergillus oryzae thia, which is involved in thiamine synthesis. one of the two introns in its 5'-untranslated region (5'-utr) contains motifs (regions a and b) highly conserved among fungal thiamine biosynthesis genes. deletion of either region relieved the repression by thiamine and thiamine inhibited intron splicing, suggesting that regions a and b are required for efficient splicing. furthermore, transcript splicing was essential for thia gene expression. these ... | 2003 | 14675766 |
| cloning, heterologous expression, and characterization of thielavia terrestris glucoamylase. | thielavia terrestris is a soil-borne thermophilic fungus whose molecular/ cellular biology is poorly understood. only a few genes have been cloned from the thielavia genus. we detected an extracellular glucoamylase in culture filtrates of t. terrestris and cloned the corresponding glaa gene. the coding region contains five introns. based on the amino acid sequence, the glucoamylase was 65% identical to neurospora crassa glucoamylase. sequence comparisons suggested that the enzyme belongs to the ... | 2003 | 14665735 |
| influence of rumen protein degradability on productive and reproductive performance in buffalo cows. | the present study aimed to ascertain the influence of crude protein (cp) digestibility in the rumen on the quantity and quality of milk production and reproductive performance, blood (bu) and milk (mu) urea, haematological profile and vaginal mucus urea, ammonia and potassium of buffalo cows. lactating buffaloes (n = 84), 60 days in milk, were randomly subdivided into group c (control, n = 42) and group t (fed a diet supplemented with aspergillus oryzae, n = 42). in three fistulated buffalo, the ... | 2003 | 15141439 |
| characteristics of a newly isolated fungus geotrichum candidum dec 1 with broad degradation spectrum of xenobiotic compounds. | a newly isolated fungus, geotrichum candidum dec 1 (abbreviated as dec 1), was found to have the ability to degrade many xenobiotic compounds such as synthetic dyes, food coloring agents, molasses, organic halogens, lignin and kraft pulp effluents. the broad spectrum of the degradation of these compounds are associated mainly with peroxidases produced by the fungus. | 2003 | 15296177 |
| molecular breeding of the mureka-non-forming sake koji mold from aspergillus oryzae by the disruption of the mrea gene. | mureka-non-forming sake koji molds were constructed from an aspergillus oryzae industrial strain by the disruption of the mrea gene using a host-vector system with the ptra gene as a dominant selectable marker. all of the mrea gene disruptants obtained retained the advantages of the host strain in terms of the brewing characteristics, while their isoamyl alcohol oxidase (iaaod) activities were significantly lower than that of the host strain. sake brewing was successfully carried out using the k ... | 2003 | 16233364 |
| dffa gene from aspergillus oryzae encodes l-ornithine n5-oxygenase and is indispensable for deferriferrichrysin biosynthesis. | we identified and analyzed the dffa gene from aspergillus oryzae which encodes l-ornithine n5-oxygenase involved in the biosynthesis of deferriferrichrysin, a type of siderophore which is a low-molecular-weight iron chelating compound. from among more than 20,000 clones in an a. oryzae est (expressed sequence tag) library, we found only one clone encoding a protein that exhibited homology to theustilago maydis sid1 protein (sid1) and pseudomonas aeruginosa pvda protein (pvda), both known as the ... | 2003 | 16233371 |
| synergistic degradation of arabinoxylan with alpha-l-arabinofuranosidase, xylanase and beta-xylosidase from soy sauce koji mold, aspergillus oryzae, in high salt condition. | this study addresses induction and some properties of alpha-l-arabinofuranosidase from a soy sauce koji mold, aspergillus oryzae hl15, cultured on solid and liquid media. alpha-l-arabinofuranosidase was induced by soybean polysaccharide and secreted into media on solid cultivation; the enzyme was associated with mycelium as a cell-wall-bound form in liquid cultivation. a major alpha-l-arabinofuranosidase, which was purified homogeneously on sds-page, showed highest activity in the presence of 10 ... | 2003 | 16233386 |
| production and properties of phytase and acid phosphatase from a sake koji mold, aspergillus oryzae. | we identified three types of acid phosphatase (acp-i, acp-ii, and acp-iii) produced by aspergillus oryzae in a submerged culture using only phytic acid as the phosphorous substrate. the optimum ph for the activities of the three enzymes was in the range of 4.5 to 5.5. analysis of the substrate specificities of these enzymes revealed that acp-i and acp-iii were acid phosphatases, and acp-ii was a phytase. these enzymes were produced during different periods of mycelial growth: acp-ii was produced ... | 2003 | 16233418 |
| production of two types of phytase from aspergillus oryzae during industrial koji making. | in our previous study, it was determined that phytase produced by aspergillus oryzae plays an important role in supplying phosphate to yeast in the process of making sake. during koji making, two types of phytase (phy-i and phy-ii) are produced. the purified phytases have high thermal and ph stability, in comparison to phytase purified from a submerged culture (acp-ii). in the present study, phy-i and phy-ii retained their activities for 45 h. the nh2-terminal sequence of phy-1, which is eight a ... | 2003 | 16233440 |
| overexpression of aspergillus aculeatus cellobiohydrolase i in aspergillus oryzae. | to express the cbhi gene, encoding aspergillus aculeatus cellobiohydrolase i (cbhi), in aspergillus oryzae, a plasmid was constructed. the strain that displayed the strongest cbhi activity among the transformants produced about 941 mg/l in liquid culture. it was confirmed by a pcr method that the plasmid was integrated at the niad locus. | 2003 | 16233469 |
| [analysis of kojic acid in aspergillus oryzae ferment by ion-pair reversed-phase high performance liquid chromatography]. | a new and an efficient method for analyzing kojic acid in aspergillus oryzae ferment by ion-pair reversed-phase high performance liquid chromatography was developed. the kojic acid was well separated on a c18 column using methanol-0.01 mol/l disodium hydrogen phosphate and 2 mmol tetrabutylammonium bromide (4:96) as the mobile phase, followed by an uv detection at 226 nm. the detection limit was 0.012 microgram/ml. the spiked recoveries in wort-yeast culture, wort-peptone culture and potato-yeas ... | 2003 | 14535109 |
| [the action of s1 nuclease and a cloning strategy for microcircular dnas]. | s1 nuclease (from aspergillus oryzae) is a specific enzyme to degrade single stranded dna or rna molecules. it has been reported to be able to convert superhelical circular dna molecules into open circle or linear forms under certain conditions, but this function has not been well explored. in order to use the action of s1 nuclease to linearize circular dna and develop a novel way of cloning microcircular dnas, the puc19 was used to investigate the relationship between the linearization efficien ... | 2003 | 15966330 |
| mycotoxin production in wheat grains by different aspergilli in relation to different relative humidities and storage periods. | four different aspergilli (aspergillus oryzae, a. parasiticus, a. terreus and a. versicolor) were grown on wheat grains underdifferent degrees of relative humidity 14, 50, 74, 80 and 90%. samples of wheat grains were taken monthly for a period of six months and examined for mycotoxin production. a. oryzae was found to produce aflatoxins b1, b2, zearalenone, don and t-2 toxins under elevated degrees of humidity and prolonged periods of storage. a. parasiticus produced aflatoxins b1, g1, niv, don ... | 2003 | 12653428 |
| electrophoretically mediated reaction of glycosidases at a nanoliter scale. | we have investigated electrophoretically mediated microanalysis (emma) for the assay of a native glyco-enzyme. as a representative of this class of enzyme, beta-glucosidase was selected, and the reaction was analyzed. our emma was based on the plug-plug interaction of enzyme and substrate plugs, which is essential to reduce quantities of materials. furthermore, we have addressed the problem of incompatibility of the enzymatic reaction and separation of the reactants. as a result, emma of native ... | 2003 | 12658703 |
| phya gene product of aspergillus ficuum and peniophora lycii produces dissimilar phytases. | phya gene products of aspergillus ficuum (af) and peniophora lycii (pl) as expressed in industrial strains of aspergillus niger and aspergillus oryzae, respectively, were purified to homogeneity and then characterized for both physical and biochemical properties. the pl phytase is 26 amino acid residues shorter than the af phytase. dynamic light scattering studies indicate that the active af phytase is a monomer while the pl phytase is a dimer. while both of the phytases retained four identical ... | 2003 | 12659840 |
| inducive effect of cresoquinone on microbiological transformation of l-tyrosine to 3,4 dihydroxy phenyl l-alanine by aspergillus oryzae ng-11(p1). | the present work describes the inducive effect of cresoquinone on microbiological transformation of l-tyrosine to 3,4 dihydroxy phenyl l-alanine ( l-dopa) by aspergillus oryzae ng-11(p1). mould mycelium was used for biochemical conversion of l-tyrosine to l-dopa because tyrosinases, beta-carboxylases and tyrosine hydroxylases are intracellular enzymes. the maximum conversion of l-tyrosine to l-dopa (0.428 mg/ml) was achieved after 60 min of biochemical reaction. to enhance the production of l-do ... | 2003 | 12664148 |
| enzymatic preparation of ginsenosides rg2, rh1, and f1. | during investigation of the hydrolysis of a protopanaxatriol-type saponin mixture by various glycoside hydrolases, crude preparations of beta-galactosidase from aspergillus oryzae and lactase from penicillium sp. were found to produce two minor saponins, ginsenoside rg(2) [6-o-(alpha-l-rhamnopyranosyl-(1-->2)-beta-d-glucopyranosyl)-20(s)-protopanaxatriol] and ginsenoside rh(1) (6-o-beta-d-glucopyranosyl-20(s)-protopanaxatriol), respectively, in high yields. moreover, a naringinase preparation fr ... | 2003 | 12672992 |
| enzymatic preparation of ginsenosides rg2, rh1, and f1 from protopanaxatriol-type ginseng saponin mixture. | during investigations on the hydrolysis of a protopanaxatriol-type saponin mixture by various glycoside hydrolases, it was found that two minor saponins, ginsenosides rg 2 and rh 1, were formed in high yields by crude beta-galactosidase from aspergillus oryzae and crude lactase from penicillium sp., respectively. moreover, a crude preparation of naringinase from penicillium decumbens readily hydrolyzed a protopanaxatriol-type saponin mixture to give an intestinal bacterial metabolite, ginsenosid ... | 2003 | 12677539 |
| fungal beta-n-acetylhexosaminidases with high beta-n-acetylgalactosaminidase activity and their use for synthesis of beta-galnac-containing oligosaccharides. | about 60 fungal strains were tested for production of extracellular beta-n-acetylhexosaminidases. a unique beta-n-acetylhexosaminidase with the beta-galnac-ase/beta-glcnac-ase ratio of 2.3-2.8 was found in the culture filtrates of some strains of penicillium oxalicum. addition of 20% (w/v) mgso(4) increased the beta-galnac-ase/beta-glcnac-ase ratio to the value of 3.35. cultivation conditions influence this ratio as well. beta-n-acetylhexosaminidases from p. oxalicum ccf 2430 and aspergillus ory ... | 2003 | 12681926 |
| functional analysis of the calcineurin-encoding gene cnaa from aspergillus oryzae: evidence for its putative role in stress adaptation. | the presence of putative stre (stress response regulatory element) and hsf (heat-shock factor) transcription factor binding sites in the promoter region of the gene encoding calcineurin ( cnaa) from aspergillus oryzae implicated a probable role for calcineurin in the stress response. the activity of calcineurin was enhanced during growth of the wild-type strain in the presence of 1 m nacl (2.6-fold), at alkaline ph 10.0 (2.9-fold) and at 37 degrees c (1.6-fold). the induction of cnaa antisense e ... | 2003 | 12709783 |
| pulsed addition of limiting-carbon during aspergillus oryzae fermentation leads to improved productivity of a recombinant enzyme. | fungal morphology in many filamentous fungal fermentations leads to high broth viscosity which limits oxygen mass transfer, and often results in reduced productivity. the objective in this study was to determine if a simple, fed-batch, process strategy-pulsed addition of limiting-carbon source-could be used to reduce fungal broth viscosity, and increase productivity of an industrially relevant recombinant enzyme (glucoamylase). as a control, three aspergillus oryzae fed-batch fermentations were ... | 2003 | 12569630 |
| molecular characterisation and expression analysis of the first hemicellulase gene (bxl1) encoding beta-xylosidase from the thermophilic fungus talaromyces emersonii. | the gene coding for beta-xylosidase, bxl1, has been cloned from the thermophilic filamentous fungus, talaromyces emersonii. this is the first report of a hemicellulase gene from this novel source. at the genomic level, bxl1 consists of an open reading frame of 2388 nucleotides with no introns that encodes a putative protein of 796 amino acids. the bxl1 translation product contains a signal peptide of 21 amino acids that yields a mature protein of 775 amino acids, with a predicted molecular mass ... | 2003 | 12763033 |
| single-strand-specific nucleases. | single-strand-specific nucleases are multifunctional enzymes and widespread in distribution. their ability to act selectively on single-stranded nucleic acids and single-stranded regions in double-stranded nucleic acids has led to their extensive application as probes for the structural determination of nucleic acids. intracellularly, they have been implicated in recombination, repair and replication, whereas extracellular enzymes have a role in nutrition. although more than 30 single-strand-spe ... | 2003 | 12586391 |
| crystal structures of aspergillus oryzae aspartic proteinase and its complex with an inhibitor pepstatin at 1.9a resolution. | the x-ray structures of aspergillus oryzae aspartic proteinase (aoap) and its complex with inhibitor pepstatin have been determined at 1.9a resolution. aoap was crystallized in an orthorhombic system with the space group p2(1)2(1)2(1) and cell dimensions of a=49.4a, b=79.4a, and c=93.6a. by the soaking of pepstatin, crystals are transformed into a monoclinic system with the space group c2 and cell dimensions of a=106.8a, b=38.6a, c=78.7a, and beta=120.3 degrees. the structures of aoap and aoap/p ... | 2003 | 12595261 |
| effect of deletion of chitin synthase genes on mycelial morphology and culture viscosity in aspergillus oryzae. | the objective of this study was to quantify the effect of disrupting two chitin synthases, chsb and csma, on the morphology and rheology during batch cultivation of aspergillus oryzae. the rheological properties were characterized in batch cultivations at different biomass concentrations (from 3.4-22.5 g kg(-1) biomass) and the power-law model adequately described the rheological properties. in the cultivations there were pellets, clumps, and freely dispersed hyphal elements. the different morph ... | 2003 | 12514801 |
| aorsin, a novel serine proteinase with trypsin-like specificity at acidic ph. | a proteinase that hydrolyses clupeine and salmine at acidic ph, called aorsin, was found in the fungus aspergillus oryzae. purified aorsin also hydrolysed benzyloxycarbonyl-arg-arg-4-methylcoumaryl-7-amide optimally at ph 4.0. the specificity of aorsin appeared to require a basic residue at the p(1) position and to prefer paired basic residues. aorsin activated plasminogen and converted trypsinogen to trypsin. the trypsin-like activity was inhibited strongly by antipain or leupeptin, but was not ... | 2003 | 12519073 |
| dipeptidyl peptidase iv on activated t cells as a target molecule for therapy of rheumatoid arthritis. | the extracellular domain of the t cell co-stimulatory molecule cd26 possesses dipeptidyl peptidase iv (dp iv) enzyme activity. activated t cells are known to increase expression of cell surface dp iv and some specific inhibitors of this enzyme have been reported to suppress t cell function. previously we have identified a dp iv inhibitor, designated tmc-2, found in culture supernatant of aspergillus oryzae. administration of tmc-2 to rats with adjuvant arthritis caused marked suppression of paw ... | 2003 | 12519388 |
| change in maltose- and soluble starch-hydrolyzing activities of chimeric alpha-glucosidases of mucor javanicus and aspergillus oryzae. | the chimeric alpha-glucosidases of mucor javanicus and aspergillus oryzae, which has high activity toward not only maltooligosaccharides but also soluble starch and has high activity toward maltooligosaccharides but faint activity toward soluble starch, respectively, were constructed by shuffling the c-terminal regions where low homology is observed between the two enzymes. the chimera genes were expressed in pichia pastoris to produce and secrete the enzymes that have predicted molecular masses ... | 2003 | 12535604 |
| sequence comparison of aflr from different aspergillus species provides evidence for variability in regulation of aflatoxin production. | aflatoxin contamination of foods and feeds is a world-wide agricultural problem. aflatoxin production requires expression of the biosynthetic pathway regulatory gene, aflr, which encodes a cys6zn2-type dna-binding protein. homologs of aflr from aspergillus nomius, bombycis, parasiticus, flavus, and pseudotamarii were compared to investigate the molecular basis for variation among aflatoxin-producing taxa in the regulation of aflatoxin production. variability was found in putative promoter consen ... | 2003 | 12553937 |
| pulsed feeding during fed-batch fungal fermentation leads to reduced viscosity without detrimentally affecting protein expression. | the goal in this study was to determine if pulsed addition of substrate could be used to alter filamentous fungal morphology during fermentation, to result in reduced broth viscosity. in all experiments, an industrially relevant strain of aspergillus oryzae was grown in 20-liter fermentors. as a control, cultures were fed limiting substrate (glucose) continuously. tests were performed by altering the feeding strategy so that the same total amount of glucose was fed in repeated 300-s cycles, with ... | 2003 | 12474257 |
| kinetic analysis of enhanced thermal stability of an alkaline protease with engineered twin disulfide bridges and calcium-dependent stability. | the thermal stability of a cysteine-free alkaline protease (alp) secreted by the eukaryote aspergillus oryzae was improved both by the introduction of engineered twin disulfide bridges (cys-69/cys-101 and cys-169/cys-200), newly constructed as part of this study, and by the addition of calcium ions. we performed an extensive kinetic analysis of the increased thermal stability of the mutants as well as the role of calcium dependence. the thermodynamic activation parameters for irreversible therma ... | 2003 | 12451555 |
| natural killer cell activity, lymphocyte proliferation, and cytokine profile in tumor-bearing mice treated with mapa, a magnesium aggregated polymer from aspergillus oryzae. | the present study examined the effects of mapa, an antitumor aggregated polymer of protein magnesium ammonium phospholinoleate-palmitoleate anhydride, isolated from aspergillus oryzae, on concanavalin a (con a)-induced spleen cell proliferation, cytokine production and on natural killer (nk) cell activity in ehrlich ascites tumor-bearing mice. the ehrlich ascites tumor (eat) growth led to diminished mitogen-induced expansion of spleen cell populations and total nk activity. this was accompanied ... | 2003 | 19180795 |
| selective induction, purification and characterization of a laccase isozyme from the basidiomycete trametes sp. ah28-2. | the white-rot fungus trametes sp. ah28-2 can synthesize extracellular laccase by induction in cellobiose-based liquid culture medium. both yields and composition of laccase isozymes, produced by trametes sp. ah28-2, would be quite different with induction by different small-molecule aromatic compounds, o-toluidine, guaiacol and 3,5-dihydroxytoluene, which affected microbial growth and the synthesis of laccase isozymes differentially. higher concentrations of the three inducers could considerably ... | 2004 | 21148825 |
| characterization of aspergillus oryzae fermentation extract effects on the rumen fungus neocallimastix frontalis, eb 188. part 1. zoospore development and physiology. | experiments were performed to determine the effect of aspergillus oryzae (ao) fermentation extract on zoospore development in the rumen fungus neocallimastix frontalis eb 188. powdered product, or liquid extract prepared from such powder, was added at the recommended value for supplementation in dairy cattle. stationary and stirred cultures were periodically sampled and assayed for extracellular and intracellular protein and enzymes, gas production, zoospore production and maturation, and carbon ... | 2004 | 12690417 |
| characterization of aspergillus oryzae fermentation extract effects on the rumen fungus neocallimastix frontalis, eb 188. part 2. carbon source utilization and effects on zoospore production. | the effect of a commercial aspergillus oryzae fermentation extract on the utilization of carbon source and zoospore production by the rumen fungus neocallimastix frontalis eb 188 was determined. in addition, the composition of a soluble extract prepared from the commercial product was analyzed. this extract was added to n. frontalis eb 188 cultures grown on a variety of substrates and periodically assayed for protein, enzymes, zoospore production, and carbon source utilization. the powdered prod ... | 2004 | 12690418 |
| hereditary variation and genetic recombination in koji-molds (aspergillus oryzae and asp. sojae). i. natural variation. | natural variation in monospore lines of koji-molds (asp. oryzae and asp. sojae), isolated from commercial koji material or soil and from laboratory stock cultures, has been observed. we can divide the 58 strains of koji-molds investigated into two groups; one group consists of inconstant strains which are very liable to produce natural variants, and the other consists of strains which remain constant through successive single spore culture. the inconstant strains develop colonies bearing various ... | 2004 | 15965886 |
| bread eating induced oral angioedema due to alpha-amylase allergy. | inhalation of dust from different enzymes can be the cause of occupational asthma in exposed workers. enzymes from different sources are being increasingly used in food. few cases of food allergy to alpha-amylase induced by eating bread have been reported. those cases were reported in bakery-related patients and in a pharmaceutical-industry worker. a 25-year-old farmer suffered sneezing, rhinorrhea, oropharyngeal itching, hoarseness, cough, and non-wheezy dyspnea after eating white bread. skin p ... | 2004 | 15736722 |
| hiochic acid, a new growth factor for lactobacillus homohiochi and lactobacillus heterohiochi. | 2004 | 15965887 | |
| growth of aspergillus oryzae during treatment of cassava starch processing wastewater with high content of suspended solids. | aspergillus oryzae ifo 30113 was used for the treatment of the cassava starch processing (csp) wastewater. the observations on the fungal morphology showed that, in the shake flasks containing the csp wastewater with the high concentration of suspended solids, the formation of pellets originated from the adherence of germinated spores to solid particles in medium. the attached solid particles were also digested during the fungal fermentation and resulted in the formation of the smooth and hollow ... | 2004 | 16233638 |
| cloning of a novel tyrosinase-encoding gene (melb) from aspergillus oryzae and its overexpression in solid-state culture (rice koji). | we have cloned a novel tyrosinase-encoding gene (melb) specifically expressed in solid-state culture of aspergillus oryzae. a tyrosinase-encoding gene (melo) from a. oryzae was already cloned and the protein structures of its catalytic and copper binding domains were investigated. however, our recent results revealed that the melo gene was highly expressed in submerged culture but not in solid-state culture. because tyrosinase activity was also detected in solid-state culture, we assumed that an ... | 2004 | 16233650 |
| cloning and expression of a nad+-dependent xylitol dehydrogenase gene (xdha) of aspergillus oryzae. | xdha, which encodes a xylitol dehydrogenase gene, was cloned from aspergillus oryzae genomic dna. it consists of 1214 bp structural region, which is interrupted by two introns, and encodes 358-amino-acid protein (38,197 da). it is similar to the known nad(+)-dependent xylitol dehydrogenase (ec 1.1.1.9). the gene was expressed in escherichia coli bl21-ai using a t7 promoter. the cell-free extract of the transformant showed a 36.5 kda band upon sds-page and nad(+)-dependent xylitol dehydrogenase a ... | 2004 | 16233653 |
| isolation and characterization of a novel gene encoding alpha-l-arabinofuranosidase from aspergillus oryzae. | we cloned and characterized a novel gene (abfa) encoding alpha-l-arabinofuranosidase (alpha-l-afase) from aspergillus oryzae. one clone homologous to the alpha-l-afase gene of thermotoga maritima was found in an expressed sequence tag (est) library of a. oryzae and a corresponding gene was isolated. molecular analysis showed that the abfa gene carried six exons interrupted by five introns and had an open reading frame encoding 481 amino acid residues. the amino acid sequence similarity at active ... | 2004 | 16233670 |
| characteristics of alpha-glucosidase production from recombinant aspergillus oryzae by membrane-surface liquid culture in comparison with various cultivation methods. | alpha-glucosidase was produced using recombinant aspergillus oryzae by membrane-surface liquid culture (mslc), a method previously developed by the authors and the results compared with other methods, including shaking flask culture (sfc), agar-plate culture (apc), culture on urethane sponge supports (usc), and liquid surface culture (lsc) to determine possible reasons for the advantageous features of mslc. when yeast extract was used as a nitrogen source, the amount of enzyme produced by mslc w ... | 2004 | 16233690 |
| a novel amine oxidase-encoding gene from aspergillus oryzae. | we cloned a novel gene (aoxa) encoding amine oxidase (aox) from aspergillus oryzae. one cdna clone showing extreme homology to the aox-encoding genes was found in an expressed sequence tag (est) library of a. oryzae. molecular analysis revealed that the aoxa carried four exons interrupted by three introns and had an open reading frame encoding 672 amino acid residues. the deduced amino acid sequence showed about 83.5% identity to the aspergillus niger ao-i. the strictly conserved residues for co ... | 2004 | 16233720 |
| reversible and strong immobilization of proteins by ionic exchange on supports coated with sulfate-dextran. | new and strong ionic exchange resins have been prepared by the simple and rapid ionic adsorption of anionic polymers (sulfate-dextran) on porous supports activated with the opposite ionic group (deae/manae). ionic exchange properties of such composites were strongly dependent on the size of the ionic polymers as well as on the conditions of the ionic coating of the solids with the ionic polymers (optimal conditions were 400 mg of sulfate-dextran 5000 kda per gram of support). around 80% of the p ... | 2004 | 15296440 |
| disordered cell integrity signaling caused by disruption of the kexb gene in aspergillus oryzae. | we isolated the kexb gene, which encodes a subtilisin-like processing enzyme, from a filamentous fungus, aspergillus oryzae. to examine the physiological role of kexb in a. oryzae, we constructed a kexb disruptant (deltakexb), which formed shrunken colonies with poor generation of conidia on czapek-dox (cd) agar plates and hyperbranched mycelia in cd liquid medium. the phenotypes of the deltakexb strain were restored under high osmolarity in both solid and liquid culture conditions. we found tha ... | 2004 | 15302836 |
| evaluation of certain food additives and contaminants. | this report represents the conclusions of a joint fao/who expert committee convened to evaluate the safety of various food additives, with a view to recommending acceptable daily intakes (adis) and to prepare specifications for the identity and purity of food additives. the first part of the report contains a general discussion of the principles governing the toxicological evaluation of food additives (including flavouring agents) and contaminants, assessments of intake, and the establishment an ... | 2004 | 15354533 |
| translation efficiency mediated by the 5' untranslated region greatly affects protein production in aspergillus oryzae. | we demonstrate that the 5' untranslated region (5'utr) plays an important role in determining translation efficiency in aspergillus oryzae, using a model beta-glucuronidase (gus) expression system. alterations in the 5' utr resulted in an increase in gus activity of up to eight-fold, without affecting mrna levels. moreover, using the most effective 5'utr construct, we could achieve remarkable intracellular overproduction of gus protein; and the gus level reached more than 50% of the total solubl ... | 2004 | 15309336 |
| hydrolytic polyketide shortening by ayg1p, a novel enzyme involved in fungal melanin biosynthesis. | the pentaketide 1,3,6,8-tetrahydroxynaphthalene (t4hn) is a key precursor of 1,8-dihydroxynaphthalene-melanin, an important virulence factor in pathogenic fungi, where t4hn is believed to be the direct product of pentaketide synthases. we showed recently the involvement of a novel protein, ayg1p, in the formation of t4hn from the heptaketide precursor ywa1 in aspergillus fumigatus. to investigate the mechanism of its enzymatic function, ayg1p was purified from an aspergillus oryzae strain that o ... | 2004 | 15310761 |
| release of short and proline-rich antihypertensive peptides from casein hydrolysate with an aspergillus oryzae protease. | angiotensin-i converting enzyme inhibitory activities were measured after hydrolysis of casein by 9 different commercially available proteolytic enzymes. among these enzymes, a protease isolated from aspergillus oryzae showed the highest angiotensin-i converting enzyme inhibitory activity per peptide. the a. oryzae peptide also showed the highest antihypertensive effect in spontaneously hypertensive rats when the systolic blood pressure was measured 5 h after oral administration of 32 mg/kg of v ... | 2004 | 15377596 |
| isolation of a novel promoter for efficient protein production in aspergillus oryzae. | a novel protein overexpression system of aspergillus oryzae was constructed. five promoters which originate from a. oryzae expressed sequence tag (est) clones in submerged culture were obtained by genome walking. these were subjected to beta-glucuronidase (gus) reporter assays. the promoter of manganese superoxide dismutase-encoding gene (sodm) showed the most gus production. the sodm gene was abundantly expressed in submerged culture but little expressed in solid-state culture. the sodm promote ... | 2004 | 15388959 |
| mode of amyr binding to the cggn8agg sequence in the aspergillus oryzae taag2 promoter. | amyr is a transcriptional activator in aspergillus spp. necessary for induction of the amylolytic enzyme genes. it recognizes 5'-cggn8cgg-3' conserved in a number of the amylolytic gene promoters, and in addition 5'-cggaaatttaa-3' in the a. oryzae alpha-amylase promoter. in this report, interaction of amyr with the 5'-cggaaatttaa-3' type binding site in the taka-amylase gene (taag2) promoter was precisely characterized by dnase i footprinting analysis and electrophoretic mobility shift assay in ... | 2004 | 15388966 |
| enzymatic synthesis of a beta-d-galactopyranosyl cyclic tetrasaccharide by beta-galactosidases. | the galactosyl transfer reaction to cyclo-[-->6)-alpha-d-glcp-(1-->3)-alpha-d-glcp-(1-->6)-alpha-d-glcp-(1-->3)-alpha-d-glcp-(1-->] (cts) was examined using lactose as a donor and beta-galactosidases from aspergillus oryzae and bacillus circulans. the a. oryzae beta-galactosidase produced three galactosyl derivatives of cts. the main galactosyl derivative produced by the a. oryzae enzyme was identified as 6-o-beta-d-galactopyranosyl-cts, cyclo-[-->6)-alpha-d-glcp-(1-->3)-[beta-d-galp-(1-->6)]-al ... | 2004 | 15183734 |
| degradation of raw or film-incorporated beta-cyclodextrin by enzymes and colonic bacteria. | beta-cyclodextrin (beta-cd) is a suitable excipient for peroral use, which improves the solubility of lipophilic drugs, as well as for colon-specific drug release when it is mixed with coating polymers. the first aim of this work was to examine the suitability of various enzymes as a simple in vitro model for the glycolytic activity in the human colon. alpha-amylase (source aspergillus oryzae) and taka diastase (source a. oryzae) showed remarkable degradation capacity of free beta-cd, whereas ot ... | 2004 | 15207542 |
| production of fructosyl transferase by aspergillus oryzae cfr 202 in solid-state fermentation using agricultural by-products. | fructosyl transferase (ftase) production by aspergillus oryzae cfr 202 was carried out by solid-state fermentation (ssf), using various agricultural by-products like cereal bran, corn products, sugarcane bagasse,cassava bagasse (tippi) and by-products of coffee and tea processing. the ftase produced was used for the production of fructo-oligosaccharides (fos), using 60% sucrose as substrate. among the cereal bran used, rice bran and wheat bran were good substrates for ftase production by a. oryz ... | 2004 | 15221221 |
| efficient degradation of tannic acid by black aspergillus species. | a set of aspergillus strains from culture collections and wild-type black aspergilli isolated on non-selective media were used to validate the use of media with 20% tannic acid for exclusive and complete selection of the black aspergilli. the 20% tannic acid medium proved useful for both quantitative and qualitative selection of all different black aspergilli, including all recognized species: a. carbonarius, a. japonicus, a. aculeatus, a foetidus, a. heteromorphus, a. niger, a. tubingensis and ... | 2004 | 15449597 |
| development of a novel quadruple auxotrophic host transformation system by argb gene disruption using adea gene and exploiting adenine auxotrophy in aspergillus oryzae. | we previously designed a triple auxotrophic host-vector system in aspergillus oryzae by isolating red-colored adenine auxotrophic mutants upon uv mutagenesis of a double auxotrophic host (niad-sc-). in the present study an effort to exploit this system and construct a novel quadruple auxotrophic host was made by disrupting the argb gene involved in arginine biosynthesis. the argb gene-disruption cassette was generated by fusion pcr, which required only two steps of pcr to insert the selectable m ... | 2004 | 15451104 |
| functional analysis of fructosyl-amino acid oxidases of aspergillus oryzae. | three active fractions of fructosyl-amino acid oxidase (faod-ao1, -ao2a, and -ao2b) were isolated from aspergillus oryzae strain rib40. n-terminal and internal amino acid sequences of faod-ao2a corresponded to those of faod-ao2b, suggesting that these two isozymes were derived from the same protein. faod-ao1 and -ao2 were different in substrate specificity and subunit assembly; faod-ao2 was active toward n(epsilon)-fructosyl n(alpha)-z-lysine and fructosyl valine (fru-val), whereas faod-ao1 was ... | 2004 | 15466528 |
| biodegradable water absorbent synthesized from bacterial poly(amino acid)s. | biodegradable hydrogels prepared by gamma-irradiation from microbial poly(amino acid)s have been studied. ph-sensitive hydrogels were prepared by the gamma-irradiation of poly(gamma-glutamic acid) (pga) produced by bacillus subtilis and poly(epsilon-lysine) (pl) produced by streptomyces albulus in aqueous solutions. when the gamma-irradiation dose was 19 kgy or more, and the concentration of pga in water was 2 wt.-% or more, transparent hydrogels could be produced. for the 19 kgy dose, the produ ... | 2004 | 15468223 |
| impact of fermented brown rice with aspergillus oryzae (febra) intake and concentrations of polybrominated diphenylethers (pbdes) in blood of humans from japan. | the isotope dilution technique was applied for the analysis of new polybrominated diphenylethers (pbdes) calibration standard (both labeled and non-labeled) using high-resolution gas chromatography-high-resolution mass spectrometry (hrgc-hrms). the relative response factor (rrf) and relative standard deviation (rsd) for new calibration standard in finnigan thermo electron (mat-95xl) and micromass (autospec ultima) were more or less identical with mean rrf (0.9882), rsd (0.0865) and cv% of (8.75) ... | 2004 | 15488571 |
| n-glycosylated catalytic unit meets o-glycosylated propeptide: complex protein architecture in a fungal hexosaminidase. | beta-n-acetylhexosaminidase from a filamentous fungus aspergillus oryzae is a secreted enzyme known to be an important component of the binary chitinolytic system. cloning of the hexa gene and sequencing of the enzyme revealed its unique preproprotein structure. while the enzyme's zincin-like and catalytic domain had significant similarities with members of the glycohydrolase 20 family, the propeptide was unique for the fungal enzyme. detailed pulse-chase and inhibition studies revealed that pro ... | 2004 | 15494009 |
| impact of febra (fermented brown rice with aspergillus oryzae) intake and concentrations of pcdds, pcdfs and pcbs in blood of humans from japan. | impact of febra (fermented brown rice with aspergillus oryzae, a rich dietary fiber) intake on the concentrations of polychlorinated-biphenyls (pcbs), -dioxin-like polychlorinated biphenyls (dlpcbs), -dibenzo-p-dioxins (pcdds), and -dibenzofurans (pcdfs) in blood of nine married japanese were studied for 2 years. concentrations of total pcbs (average+/-standard deviation) were greater 326,000+/-263,000 and 207,000+/-89,000 in febra-intake and non-febra-intake groups, respectively, on pg/g lipid ... | 2004 | 15519386 |
| enzymatic production and complete nuclear magnetic resonance assignment of the sugar lactulose. | the enzymatic transgalactosylation from lactose to fructose leading to the prebiotic disaccharide lactulose was investigated using the beta-galactosidase from aspergillus oryzae and the hyperthermostable beta-glycosidase from pyrococcus furiosus (celb). the conditions for highest lactulose yields relative to the initial lactose concentration were established on a 1 ml scale. dependent on the initial molar ratio of lactose to fructose, more or fewer oligosaccharides other than lactulose were gene ... | 2004 | 15537307 |
| preventive effect of fermented brown rice and rice bran on n-nitrosomethylbenzylamine-induced esophageal tumorigenesis in rats. | modifying effect of fermented brown rice by aspergillus oryzae (fbra) during the initiation or post-initiation phase of n-nitrosomethylbenzylamine (nmba)-induced esophageal tumorigenesis was investigated in rats. five-week-old male f344 rats were divided into 7 groups, and groups 1-5 were given subcutaneous injections of nmba (0.5 mg/kg body weight/injection 15 times) for 5 weeks starting at 7 weeks of age. groups 2 and 4 were fed the diet containing 5 and 10% fbra during the initiation phase, r ... | 2004 | 15547721 |
| detection of aflatoxin-producing molds in korean fermented foods and grains by multiplex pcr. | an assay based on multiplex pcr was applied for the detection of potential aflatoxin-producing molds in korean fermented foods and grains. three genes, avfa, omta, and ver-1, coding for key enzymes in aflatoxin biosynthesis, were used as aflatoxin-detecting target genes in multiplex pcr. dna extracted from aspergillus flavus, aspergillus parasiticus, aspergillus oryzae, aspergillus niger, aspergillus terreus, penicillium expansum, and fusarium verticillioides was used as pcr template to test spe ... | 2004 | 15553652 |
| molecular cloning, overexpression, and purification of micrococcus luteus k-3-type glutaminase from aspergillus oryzae rib40. | we have for the first time found and cloned the cdna (aoglsa) of aspergillus oryzae rib40, which encodes a 49.9-kda protein sharing 40% homology with the salt-tolerant glutaminase of micrococcus luteus k-3 (micrococcus glutaminase). aoglsa was subcloned into a series of expression vectors and expressed in saccharomyces cerevisiae and escherichia coli. the gene product, which we named aogls, showed glutaminase activity and was produced in a cell wall fraction of s. cerevisiae and a soluble protei ... | 2004 | 15555943 |
| analysis of secreted proteins during conidial germination of aspergillus oryzae rib40. | to broaden our understanding of extracellular proteins of aspergillus oryzae at the conidial germination stage, analyses of the secreted proteins during germination were carried out. taka-amylase a (taa), glucoamylase (glaa), and aspergillopepsin a (pepa) were identified as the main products by peptide mass fingerprinting. taa and pepa were detected simultaneously with the formation of germ tubes. with the development of germination, the ph of the medium fell from 5.5 to 3.5. the secreted pepa h ... | 2004 | 15618633 |
| different control mechanisms regulate glucoamylase and protease gene transcription in aspergillus oryzae in solid-state and submerged fermentation. | solid-state fermentation (ssf) with aspergillus oryzae results in high levels of secreted protein. however, control mechanisms of gene expression in ssf have been only poorly studied. in this study we show that both glucoamylase (glab) and protease (alpa, nptb) genes are highly expressed during surface cultivation on wheat-based solid medium, and even higher during cultivation on wheat kernels. in wheat-based liquid medium, low levels of gene expression are observed. typical ssf cultivation cond ... | 2004 | 15800731 |
| bioprocess monitoring using near-infrared spectroscopy. | near-infrared spectroscopy (nir) is a nondestructive analytical technique that has been used for simultaneous prediction of the concentrations of several substrates, products and constructs in mixtures sampled from fermentation processes. in this chapter, we discuss applications of nir for the monitoring of bioprocesses involving rice vinegar, compost, glycolipid, l-glutamic acid, lactic acid fermentation, mushroom cultivation, and koji production. this includes detailed discussion of applicatio ... | 2004 | 15453190 |
| numerical analysis of the depletion zone formation around a growing protein crystal. | it is expected that a protein depletion zone and an impurity depletion zone are formed around a crystal during protein crystal growth if the diffusion field around the crystal is not disturbed. the growth rate of the crystal may be decreased and the impurity uptake may be suppressed to result in highly ordered crystals if these zones are not disturbed. it is well known that a microgravity environment can reduce convective fluid motion, and this is thought to disturb the depletion zones. therefor ... | 2004 | 15644341 |
| aspergillus oryzae gb-107 fermentation improves nutritional quality of food soybeans and feed soybean meals. | this study evaluated the effect of fermentation on the nutritional quality of food-grade soybeans and feed-grade soybean meals. soybeans and soybean meals were fermented by aspergillus oryzae gb-107 in a bed-packed solid fermentor for 48 hours. after fermentation, their nutrient contents as well as trypsin inhibitor were measured and compared with those of raw soybeans and soybean meals. proteins were extracted from fermented and non-fermented soybeans and soybean meals, and the peptide characte ... | 2004 | 15671685 |
| catalysis of proline-directed protein phosphorylation by peptidyl-prolyl cis/trans isomerases. | proline-directed protein phosphorylation was shown to depend on the capacity of the targeted ser(thr)-pro bond to exhibit conformational polymorphism. the cis/trans isomer specificity underlying erk2-catalyzed phosphate transfer leads to a complete discrimination of the cis ser(thr)-pro conformer of oligopeptide substrates. we investigated in vitro the erk2-catalyzed phosphorylation of aspergillus oryzae rnase t1 containing two ser-pro bonds both of which share high stabilization energy in their ... | 2004 | 15147846 |
| chemical constituents, antifungal and antioxidative effects of ajwain essential oil and its acetone extract. | gc and gc-ms analysis of ajwain essential oil showed the presence of 26 identified components which account for 96.3% of the total amount. thymol (39.1%) was found as a major component along with p-cymene (30.8%), gamma-terpinene (23.2%), beta-pinene (1.7%), terpinene-4-ol (0.8%) whereas acetone extract of ajwain showed the presence of 18 identified components which account for 68.8% of the total amount. the major component was thymol (39.1%) followed by oleic acid (10.4%), linoleic acid (9.6%), ... | 2004 | 15161185 |
| transcriptional analysis of genes for energy catabolism and hydrolytic enzymes in the filamentous fungus aspergillus oryzae using cdna microarrays and expressed sequence tags. | aspergillus oryzae is a fungus used extensively in the fermentation industry. we constructed cdna microarrays comprising 2,070 highly expressed cdnas selected from the approximately 6,000 non-redundant expressed sequence tags (ests) in the a. oryzae est database (http://www.aist.go.jp/riodb/ffdb/index.html). using the cdna microarrays, we analyzed the gene expression profiles of a. oryzae cells grown under the glucose-rich (ac) and glucose-depleted (an) liquid culture conditions used during the ... | 2004 | 15221230 |
| role of h164 in a unique dye-decolorizing heme peroxidase dyp. | the expression system of a unique dye-decolorizing peroxidase dyp in escherichia coli has been constructed. the molecular mass of the expressed dyp (edyp) is 47kda, indicating no any modification with saccharides. the characteristics of edyp were almost the same as those of native dyp from a fungus thanatephorus cucumeris dec 1 and recombinant dyp with aspergillus oryzae except thermostability. as h164 was suggested to be the proximal histidine based on the preliminary x-ray crystallographic ana ... | 2004 | 15313183 |
| lipase production by recombinant strains of aspergillus niger expressing a lipase-encoding gene from thermomyces lanuginosus. | two recombinant strains of aspergillus niger (nw 297-14 and nw297-24) producing a heterologous lipase from thermomyces lanuginosus were constructed. the heterologous lipase was expressed using the taka amylase promoter from aspergillus oryzae. the production kinetics of the two strains on different carbon sources in batch and carbon-limited chemostat cultivations were evaluated. in batch cultivations, the highest total product yield coefficient (y(xp total)), given as the sum of extracellular an ... | 2004 | 15316684 |
| processing of a cowpea--groundnut blend into a miso-like product. | a traditional salt-miso process was used to produce a miso-like product from a 50-50 mixture of cowpea and groundnuts in a 60-day fermentation process as part of a study to determine the suitability of local legumes as raw materials for the production of miso. the koji was freshly prepared from a locally obtained rice and aspergillus oryzae spores obtained from japan. an old miso sample also obtained from japan was used as the source of lactic acid bacteria. the physico-chemical changes in the p ... | 2004 | 15223597 |
| airborne fungi in industrial environments--potential agents of respiratory diseases. | investigations on airborne fungi in a poultry house, a swinery, a feed preparing and storing house, a grain mill, a wooden panel producing factory, and organic waste recycling facilities have been carried out in lithuania. low concentrations of fungal spores were detected in the wooden panel producing factory, the swinery, the feed preparing and storing house, and the poultry house; moderate concentrations were found in the organic waste recycling facilities; high concentrations were revealed at ... | 2004 | 15236494 |
| secretion, purification, and characterization of a recombinant aspergillus oryzae tannase in pichia pastoris. | tannase (tannin acyl hydrolase) is an industrially important enzyme produced by a large number of fungi, which hydrolyzes the ester and depside bonds of gallotannins and gallic acid esters. in the present work, a tannase from aspergillus oryzae has been cloned and expressed in pichia pastoris. the catalytic activity of the recombinant enzyme was assayed. a secretory form of enzyme was made with the aid of saccharomyces cerevisiae alpha-factor, and a simple procedure purification protocol yielded ... | 2004 | 15249037 |
| an appropriate increase in the transcription of aspergillus nidulans uvsc improved gene targeting efficiency. | gene targeting to knock out the activity of specific genes has become important due to recent progress in genomics research. but this technique is still unavailable for many organisms, including economically important microorganisms, due to the high background of ectopic integration during genetic transformation. strategies to improve targeting efficiency have included manipulating the expression of genes that are involved in homologous recombination. in this study, transcription of aspergillus ... | 2004 | 15322347 |
| enzymatic synthesis of complex glycosaminotrioses and study of their molecular recognition by hevein domains. | hevein, a protein found in hevea brasiliensis, has a crd domain, which is known to bind chitin and glcnac-containing oligosaccharides. by using nmr and molecular modeling as major tools we have demonstrated that trisaccharides containing galnac and mannac residues are also recognized by hevein domains. thus far unknown trisaccharides glcnacbeta(1-->4)glcnacbeta(1-->4)mannac (1) and galnacbeta(1-->4)glcnacbeta(1-->4)mannac (2) were synthesized with the use of beta-n-acetylhexosaminidase from aspe ... | 2004 | 15254625 |
| cloning and enhanced expression of the cytochrome p450nor gene (nica; cyp55a5) encoding nitric oxide reductase from aspergillus oryzae. | we cloned and characterized the gene and cdna of aspergillus oryzae cytochrome p450nor (anor). the anor gene (nica; cyp55a5) has a different gene structure from other p450nor genes in that it has an extra intron. there were not only two kinds of mrna but also two sets of tata-box and ccaat-box, and it appears that this gene has two expression patterns, like cyp55a1 of fusarium oxysporum. a reporter analysis using the uida gene indicated that gene expression of cyp55a5 was induced under anaerobic ... | 2004 | 15502348 |
| rnase t1 variant rv cleaves single-stranded rna after purines due to specific recognition by the asn46 side chain amide. | attempts to alter the guanine specificity of ribonuclease t1 (rnase t1) by rational or random mutagenesis have failed so far. the rnase t1 variant rv (lys41glu, tyr42phe, asn43arg, tyr45trp, and glu46asn) designed by combination of a random and a rational mutagenesis approach, however, exhibits a stronger preference toward adenosine residues than wild-type rnase t1. steady state kinetics of the cleavage reaction of the two dinucleoside phosphate substrates adenylyl-3',5'-cytidine and guanylyl-3' ... | 2004 | 15005620 |
| enantioselective transesterification using immobilized aspergillus oryzae overexpressing lipase. | in the present study, we used gene manipulation to construct a recombinant aspergillus oryzae strain overexpressing lipase and investigated its application to the optical resolution of chiral compounds. a. oryzae niad300, which was derived from the wild-type strain rib40, was used as the host strain. the tgla gene, which encodes a triacylglycerol lipase, was cloned from the a. oryzae niad300 chromosomal genome, then reintroduced, with and without a secretion-signal sequence, into the genome and ... | 2004 | 15052375 |