Publications

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production of fructooligosaccharides by beta-fructofuranosidases from aspergillus oryzae kb.aspergillus oryzae kb produces two types of beta-fructofuranosidases: f1 and f2. f1 produces the fructooligosaccharides (foss) 1-kestose, nystose, and fructosyl nystose from sucrose through a transfructosylation action, whereas f2 mainly hydrolyzes sucrose to glucose and fructose. f1 and f2 enzymes were more selectively produced from the kb strain in liquid media with a sucrose concentration>2% and <2%, respectively. immobilization using an anion-exchange resin (wa-30; polystyrene with tertiary ...201020014851
characterization of recombinant prolyl aminopeptidase from aspergillus oryzae.prolyl aminopeptidase (pap) degrades only amino-terminal proline from peptides. the food-grade fungus aspergillus oryzae produces this enzyme only in small amounts. in this paper, we present efficient production of recombinant pap with an overexpression system of a. oryzae and characterization of its biochemical properties.201020028436
distinct enzymatic and cellular characteristics of two secretory phospholipases a2 in the filamentous fungus aspergillus oryzae.microbial secretory phospholipases a(2) (spla(2)s) are among the last discovered and least known members of this functionally diverse family of enzymes. we analyzed here two spla(2)s, named splaa and splab, of the filamentous ascomycete aspergillus oryzae. splaa and splab consist of 222 and 160 amino acids, respectively, and share the conserved cys and catalytic his-asp residues typical of microbial spla(2)s. two spla(2)s differ in ph optimum, ca(2+) requirement and expression profile. the splaa ...201020045482
bioreduction of trivalent aurum to nano-crystalline gold particles by active and inactive cells and cell-free extract of aspergillus oryzae var. viridis.bioreduction efficacy of both active (ab) and inactive (ib) cells/biomass of aspergillus oryzae var. viridis and their respective cell-free extracts (ace and ice) to convert trivalent aurum to gold nanoparticles were tested in the present study. strong plasmon resonance of gold nanoparticles was observed between 540 and 560 nm in the samples obtained from ab, ib, ace and ice. transmission electron microscopy (tem), field emission scanning electron microscopy (fe-sem), energy dispersive x-ray (ed ...201020056324
characterization of the palh gene from aspergillus oryzae.we disrupted the palh gene, which is known to participate in the ambient ph signal transduction pathway, in aspergillus oryzae. palh disruption caused significant decreases in pacc expression and alkaline protease activity. hence we believe that palh plays a very important role in controlling the alkaline protease level in a. oryzae.201020057127
evaluation of enzyme mixtures in releasing fermentable sugars from pre-pulping extracts of mixed northeast hardwoods.one near-term option to developing a forest product biorefinery is to derive pre-pulping extract from incoming wood chips before the main pulping step. the release of monomer sugars from a xylan-rich extract, creating a fermentable substrate is a prerequisite for utilization of pre-pulping extract for production of ethanol or other value-added products. this study examined the individual and mixture efficiencies of two hemicellulolytic microbial enzymes and two xylanase preparations in catalyzin ...201020084471
characterization of aroma compounds in chinese rice wine qu by solvent-assisted flavor evaporation and headspace solid-phase microextraction.the aroma of chinese rice wine qu is one of the most important factors that influences the flavor of chinese rice wine. to better understand the aroma of qu, aroma compounds in four wheat qus and two xiao qus were identified by chromatography-olfactometry (gc-o) after solvent extraction followed by solvent-assisted flavor evaporation (safe). a total of 39 aroma compounds were characterized by gc-o. on the basis of aroma intensity, 1-hexanal, ethyl hexanoate, 1-octen-3-ol, and phenylacetaldehyde ...201020088505
high-level expression of lactobacillus beta-galactosidases in lactococcus lactis using the food-grade, nisin-controlled expression system nice.in this work the overlapping genes (lacl and lacm) encoding heterodimeric beta-galactosidases from lactobacillus reuteri , lb. acidophilus , lb. sakei , and lb. plantarum were cloned into two different nisin-controlled expression (nice) vectors and expressed using lactococcus lactis nz9000 and nz3900 as hosts. the lacl gene, encoding the large subunit of the beta-galactosidases, was fused translationally downstream of the nisin-inducible promoter nisa. chloramphenicol was employed as selection m ...201020092320
enhanced thermal and ultrasonic stability of a fungal protease encapsulated within biomimetically generated silicate nanospheres.dendrimers are highly branched synthetic macromolecules with a globular shape. they have been successfully used for generation of nanospheres at mild conditions via biomimetic silicification. encapsulation of enzyme molecules within these nanospheres during their synthesis is a promising method for rapid and efficient entrapment of several enzymes. however, encapsulation of proteolytic enzymes has been rarely done via biomimetic silicification. as well, the operational stability of encapsulated ...201020100547
molecular cloning, characterization and expression analysis of two beta-n-acetylhexosaminidase homologs of coccidioides posadasii.two full-length cdnas were isolated from coccidioides posadasii that encode two deduced proteins (cphex1 and cphex2) with homology to the glycosyl hydrolase 20 family of beta-n-acetylhexosaminidases. cphex1 consists of 595 amino acids, has a predicted molecular mass of 68 kda and shares the highest identity with the n-acetylhexosaminidase (naga) of aspergillus nidulans, while cphex2 consists of 603 amino acids, has a predicted molecular mass of 68.5 kda and shares the highest identity with nag1 ...201020109094
molecular strategy for identification in aspergillus section flavi.abstract aspergillus flavus is one of the most common contaminants that produces aflatoxins in foodstuffs. it is also a human allergen and a pathogen of animals and plants. aspergillus flavus is included in the aspergillus section flavi that comprises 11 closely related species producing different profiles of secondary metabolites. a six-step strategy has been developed that allows identification of nine of the 11 species. first, three real-time pcr reactions allowed us to discriminate four grou ...201020113354
l-ascorbate, a strong inducer of l-dopa (3,4-dihydroxy-l-phenylalanine) production from pre-grown mycelia of aspergillus oryzae nrrl-1560.the inductive effect of l-ascorbate on the microbiological production of l-dopa (3,4-dihydroxy-l-phenylalanine) from aspergillus oryzae nrrl-1560 was investigated. all biochemical reactions were performed aerobically using mould mycelia as a source of enzyme tyrosinase and acetate buffer (ph 3.0) as an extractant. l-tyrosine as a substrate was added at a level of 2.5 mg/ml. maximal l-dopa production (1.876 mg/ml) was achieved when l-ascorbate (5.0 mg/ml) was added 6 min after the initiation of t ...201020121699
characterization of aspergillus oryzae glycoside hydrolase family 43 beta-xylosidase expressed in escherichia coli.this is the first report of glycoside hydrolase family 43 beta-xylosidase from aspergillus oryzae. to characterize this enzyme, the recombinant enzyme was expressed in escherichia coli. unlike known beta-xylosidases from fungal origins, the enzyme did not show substrate ambiguity and was stable at alkaline ph.201020129093
analysis of wide-domain transcriptional regulation in solid-state cultures of aspergillus oryzae.many filamentous fungi secrete considerable quantities of enzymes including protease, cellulase and xylanase, which are of major industrial importance. over the past few decades, many of these fungal enzymes have been isolated and their relevant genes characterised. solid-state fermentation (ssf), an ancient technique described as a fermentation process performed on non-soluble material whereby the material acts as a physical support and as a source of nutrients, is widely employed in the produc ...201020145973
production of xylanase with a transformant of aspergillus oryzae rib40 in a liquid-surface immobilization (lsi) system.xylanase production by a xynf1 (33 kda)-transformant of aspergillus oryzae rib40 was compared between submerged cultivation (smc) and liquid-surface immobilization (lsi) systems. while the accumulation of xylanase in the smc decreased by prolonged incubation, lsi system enabled the continuation of xylanase production to afford 4.5-fold xylanase production compared with the smc system.201020159567
cultivation characteristics and gene expression profiles of aspergillus oryzae by membrane-surface liquid culture, shaking-flask culture, and agar-plate culture.we cultivated a filamentous fungus, aspergillus oryzae iam 2706 by three different cultivation methods, i.e., shaking-flask culture (sfc), agar-plate culture (apc), and membrane-surface liquid culture (mslc), to elucidate the differences of its behaviors by different cultivation methods under the same media, by measuring the growth, secretion of proteases and alpha-amylase, secreted protein level, and gene transcriptional profile by the dna microarray analysis. the protease activities detected b ...201020159576
what does genetic diversity of aspergillus flavus tell us about aspergillus oryzae?aspergillus flavus and aspergillus oryzae belong to aspergillus section flavi. they are closely related and are of significant economic importance. the former species has the ability to produce harmful aflatoxins while the latter is widely used in food fermentation and industrial enzyme production. this review summarizes the current understanding of the similarity of the a. flavus and a. oryzae genomes, the genetic diversity in a. flavus and a. oryzae populations, the causes of this diversity, a ...201020163884
characterization of water-soluble complexes of polyacrylic acid with alpha-amylase from aspergillus oryzae.polycomplex formation of alpha-amylase from aspergillus oryzae (taka) with polyacrylic acid (paa) was studied by ph titration, fluorescence, and high performance liquid chromatography (hplc) methods in water solutions. according to the our results, the complex formation and its solubility were depended on nature of enzyme and the ph of solutions. both of them correlates isoelectric points (pi). the stability of paa-amylase complexes was negligibly weak at ph 7 [ph > pi (isoelectric ph)]. stable ...201020165908
promotion of efficient saccharification of crystalline cellulose by aspergillus fumigatus swo1.swollenin is a protein from trichoderma reesei that has a unique activity for disrupting cellulosic materials, and it has sequence similarity to expansins, plant cell wall proteins that have a loosening effect that leads to cell wall enlargement. in this study we cloned a gene encoding a swollenin-like protein, swo1, from the filamentous fungus aspergillus fumigatus, and designated the gene afswo1. afswo1 has a bimodular structure composed of a carbohydrate-binding module family 1 (cbm1) domain ...201020173066
aspergillus oryzae flavohemoglobins promote oxidative damage by hydrogen peroxide.apart from their well-established role in nitric oxide detoxification, flavohemoglobins (fhbs) are also believed to be involved in protection against oxidative stress in some yeast and bacteria. however, different studies have reported contradictory results in this regard. here, we investigate the relationship between two fhbs in aspergillus oryzae (cytosolic fhb1 and mitochondrial fhb2) and oxidative stress. the strains deficient in the two fhbs exhibited higher resistance to hydrogen peroxide ...201020211603
some biochemical and physical changes during the preparation of the enzyme-ripening sufu, a fermented product of soybean curd.in this study, sufu, a fermented product of soybean curd, was prepared by ripening the salted tofu in the mash of aspergillus oryzae -fermented rice-soybean koji possessing various hydrolytic enzymes for 16 days. it was found that protease, alpha-amylase, beta-amylase, and lipase activities observed in the koji granules reduced most pronouncedly during the initial 4 or 8 days of ripening. meanwhile, an increase in the activity of the various enzymes was noted in the ripening infusion and tofu cu ...201020218723
stimulation of lignocellulosic biomass hydrolysis by proteins of glycoside hydrolase family 61: structure and function of a large, enigmatic family.currently, the relatively high cost of enzymes such as glycoside hydrolases that catalyze cellulose hydrolysis represents a barrier to commercialization of a biorefinery capable of producing renewable transportable fuels such as ethanol from abundant lignocellulosic biomass. among the many families of glycoside hydrolases that catalyze cellulose and hemicellulose hydrolysis, few are more enigmatic than family 61 (gh61), originally classified based on measurement of very weak endo-1,4-beta-d-gluc ...201020230050
mutation of key residues in the c-methyltransferase domain of a fungal highly reducing polyketide synthase.site directed mutations of the c-methyltransferase domain of squalestatin tetraketide synthase were made in an attempt to alter the methylation pattern of the synthase expressed in vivo: mutation resulted in either no effect or in complete abrogation of polyketide production.201020237645
regulation of development in aspergillus nidulans and aspergillus fumigatus.members of the genus aspergillus are the most common fungi and all reproduce asexually by forming long chains of conidiospores (or conidia). the impact of various aspergillus species on humans ranges from beneficial to harmful. for example, several species including aspergillus oryzae and aspergillus niger are used in industry for enzyme production and food processing. in contrast, aspergillus flavus produce the most potent naturally present carcinogen aflatoxins, which contaminate various plant ...201023956662
in situ generation of hydrogen peroxide by carbohydrate oxidase and cellobiose dehydrogenase for bleaching purposes.the carbohydrate oxidase from microdochium nivale (caox), heterologously expressed in aspergillus oryzae, and cellobiose dehydrogenase from myriococcum thermophilum (mtcdh), were assessed for their ability to generate bleaching species at a ph suitable for liquid detergents. the substrate specificities of caox and mtcdh were analyzed on a large variety of soluble and insoluble substrates, using oxygen as an electron receptor. even insoluble substrates like cellulose were oxidized from both caox ...201021298807
uniform culture in solid-state fermentation with fungi and its efficient enzyme production.solid-state fermentation (ssf) has attracted a lot of interest for carrying out high-level protein production in filamentous fungi. however, it has problems such as the fermentation heat generated during the culture in addition to the reduced mobility of substances. these conditions lead to a nonuniform state in the culture substrate and result in low reproducibility. we constructed a non-airflow box (nab) with a moisture permeable fluoropolymer membrane, thereby making it possible to control an ...201021163699
integrated analysis of the global transcriptional response to α-amylase over-production by aspergillus oryzae.the filamentous fungus aspergillus oryzae is an important microbial cell factory for industrial production of many enzymes, such as α-amylase. in order to optimize the industrial enzyme production process, there is a need to understand fundamental processes underlying enzyme production, here under how enzyme production links to metabolism through global regulation. through a genome-scale metabolic network for integrated analysis of transcriptome data and flux calculation, we identified key playe ...201021165907
molecular evolution and characterization of fungal indoleamine 2,3-dioxygenases.indoleamine 2,3-dioxygenase (ido) and tryptophan 2,3-dioxygenase (tdo) are tryptophan-degrading enzymes. mammalian ido expression is induced by cytokines and has antimicrobial and immunomodulatory effects. a major role of mammalian tdo is to supply nicotinamide adenine dinucleotide (nad(+)). in fungi, the ido homologue is thought to be expressed constitutively and supply nad(+), as tdo is absent from their genomes. here, we reveal the distribution of ido genes among fungal species and characteri ...201021170645
macroautophagy-mediated degradation of whole nuclei in the filamentous fungus aspergillus oryzae.filamentous fungi consist of continuum of multinucleate cells called hyphae, and proliferate by means of hyphal tip growth. accordingly, research interest has been focusing on hyphal tip cells, but little is known about basal cells in colony interior that do not directly contribute to proliferation. here, we show that autophagy mediates degradation of basal cell components in the filamentous fungus aspergillus oryzae. in basal cells, enhanced green fluorescent protein (egfp)-labeled peroxisomes, ...201021187926
regioselective deglycosylation of onion quercetin glucosides by saccharomyces cerevisiae.bioconversion of quercetin glucosides using four generally recognized as safe (gras) organisms (aspergillus oryzae, bacillus subtilis, lactobacillus plantarum, and saccharomyces cerevisiae) was evaluated by measuring changes in the levels of quercetin compounds of onion. of the four organisms, s. cerevisiae increased the content of quercetin-3-o-β-d-glucoside (iii; isoquercitrin) and quercetin (iv), whereas decreasing quercetin-3,4'-o-β-d-glucoside (i) and quercetin-4'-o-β-d-glucoside (ii). also ...201021188617
loss of aspergillus oryzae amyr function indirectly affects hemicellulolytic and cellulolytic enzyme production.aspergillus oryzae ab390, a derivative of a. oryzae or101, was found to be suitable for soy sauce production, yielding a product light brown in color. compared to the parent strain, hemicellulase and cellulase activities in the mutant were higher; however, its amylase activity was found to be much lower. to determine the cause of these differences, the enzymatic profile change, as a function of the carbon source in submerged cultures, was examined. amylase activity in ab390 was hardly detectable ...201021193346
effects of moisture enhancement, enzyme treatment, and blade tenderization on the processing characteristics and tenderness of beef semimembranosus steaks.the individual and combined effects of moisture enhancement with a salt/phosphate solution (me), blade tenderization (bt), and enzyme injection with proteinases derived from aspergillus oryzae or bacillus subtilis on cooking properties, warner-bratzler shear force (wbsf), and sensory characteristics of beef semimembranosus were investigated. me significantly (p < 0.01) reduced wbsf and increased (p < 0.05) sensory scores for juiciness and tenderness. bt increased (p < 0.05) initial and overall t ...201021194850
expression, purification and characterization of recombinant α-glucosidase in pichia pastoris.an expression plasmid containing the agda gene encoding aspergillus oryzae zl-1 α-glucosidase was constructed and expressed in pichia pastoris x-33. the molar mass of the purified protein was estimated by sds-page. hplc analysis showed that the purified enzyme has a transglucosylating activity with maltose as substrate. the main component of the enzyme products was panose, while amounts of isomaltose and isomaltotriose were very low or absent. ph 5.2 and temperature of 37 °c were optimum for enz ...201021253902
purification and characterization of an intracellular beta-glucosidase from the protoplast fusant of aspergillus oryzae and aspergillus niger.protoplasts of aspergillus oryzae 3.481 and aspergillus niger 3.316 were prepared using cellulose and snail enzyme with 0.6 m nacl as osmotic stabilizer. protoplast fusion has been performed using 35% polyethylene glycol 4.000 with 0.01 mm cacl2. the fused protoplasts have been regenerated on regeneration medium and fusants were selected for further studies. an intracellular beta-glucosidase (ec 3.2.1.21) was purified from the protoplast fusant of aspergillus oryzae 3.481 and aspergillus niger 3 ...201021254729
permanent genetic resources added to molecular ecology resources database 1 october 2009-30 november 2009.this article documents the addition of 411 microsatellite marker loci and 15 pairs of single nucleotide polymorphism (snp) sequencing primers to the molecular ecology resources database. loci were developed for the following species: acanthopagrus schlegeli, anopheles lesteri, aspergillus clavatus, aspergillus flavus, aspergillus fumigatus, aspergillus oryzae, aspergillus terreus, branchiostoma japonicum, branchiostoma belcheri, colias behrii, coryphopterus personatus, cynogolssus semilaevis, cy ...201021565039
development of vntr markers for three aspergillus species used in brewing.using a bioinformatics approach, we developed 18 variable number of tandem repeat markers for aspergillus oryzae for use in population genetic studies. repeat sequences in the genome sequences of a. oryzae were identified by a tandem repeat finding program. length polymorphisms at 18 loci were examined in 41 strains of a. oryzae. the total number of alleles per locus ranged from two to 20. investigation of cross-species amplifications with a. sojae and a. tamarii showed success. the variable num ...200921564707
cyclopiazonic acid biosynthesis of aspergillus flavus and aspergillus oryzae.cyclopiazonic acid (cpa) is an indole-tetramic acid neurotoxin produced by some of the same strains of a. flavus that produce aflatoxins and by some aspergillus oryzae strains. despite its discovery 40 years ago, few reviews of its toxicity and biosynthesis have been reported. this review examines what is currently known about the toxicity of cpa to animals and humans, both by itself or in combination with other mycotoxins. the review also discusses cpa biosynthesis and the genetic diversity of ...200922069533
identification of two aflatrem biosynthesis gene loci in aspergillus flavus and metabolic engineering of penicillium paxilli to elucidate their function.aflatrem is a potent tremorgenic toxin produced by the soil fungus aspergillus flavus, and a member of a structurally diverse group of fungal secondary metabolites known as indole-diterpenes. gene clusters for indole-diterpene biosynthesis have recently been described in several species of filamentous fungi. a search of aspergillus complete genome sequence data identified putative aflatrem gene clusters in the genomes of a. flavus and aspergillus oryzae. in both species the genes for aflatrem bi ...200919801473
acrylamide degradation by filamentous fungi used in food and beverage industries.filamentous fungi (24 strains) used in food and beverage industries were investigated for acrylamide-degradation ability: aspergillus oryzae kbn1010 showed the highest ability. little acrylic acid was produced but no glycidamide was detected during aa degradation in roasted green tea; therefore, a. oryzae could be used for reducing the aa concentration.200919804862
analysis of enzyme production by submerged culture of aspergillus oryzae using whole barley.we have reported on high enzyme production by submerged culture of aspergillus kawachii using barley with the husk (whole barley). to elucidate the mechanism underlying this high enzyme production, we performed a detailed analysis. aspergillus oryzae rib40 was submerged-cultured using whole barley and milled whole barley. enzyme production was analyzed in terms of changes in medium components and gene expression levels. when whole barley was used, high production of glucoamylase and alpha-amylas ...200919809198
structural and functional studies of aspergillus oryzae cutinase: enhanced thermostability and hydrolytic activity of synthetic ester and polyester degradation.cutinases are responsible for hydrolysis of the protective cutin lipid polyester matrix in plants and thus have been exploited for hydrolysis of small molecule esters and polyesters. here we explore the reactivity, stability, and structure of aspergillus oryzae cutinase and compare it to the well-studied enzyme from fusarium solani. two critical differences are highlighted in the crystallographic analysis of the a. oryzae structure: (i) an additional disulfide bond and (ii) a topologically favor ...200919810726
biosensor for luteolin based on silver or gold nanoparticles in ionic liquid and laccase immobilized in chitosan modified with cyanuric chloride.novel and effective biosensors based on ag or au nanoparticles dispersed in ionic liquid (il) 1-butyl-3-methylimidazolium hexafluorophosphate (bmi.pf(6)) and laccase (lac) from aspergillus oryzae immobilized in chitosan (chi) chemically cross-linked with cyanuric chloride (cc) were constructed. this enzyme catalyzes the oxidation of luteolin to the corresponding o-quinone, which is electrochemically reduced back to luteolin at 0.35 v vs. ag/agcl. square-wave voltammetry was used for the electroc ...200919838422
aspergillus oryzae atfa controls conidial germination and stress tolerance.we compared atfa and atfb, the genes encoding the respective atf/creb-type transcription factors in aspergillus oryzae. the germination ratio of deltaatfa conidia was low without any stress, unlike that of deltaatfb conidia. the deltaatfa conidia were more sensitive to oxidative stress than the deltaatfb conidia, which are also sensitive to oxidative stress. we compared the gene expressions of these strains by using a dna microarray, genechip. almost all the genes regulated by atfb were also reg ...200919770065
genes regulated by aoxlnr, the xylanolytic and cellulolytic transcriptional regulator, in aspergillus oryzae.xlnr is a zn(ii)2cys6 transcriptional activator of xylanolytic and cellulolytic genes in aspergillus. overexpression of the aoxlnr gene in aspergillus oryzae (a. oryzae xlnr gene) resulted in elevated xylanolytic and cellulolytic activities in the culture supernatant, in which nearly 40 secreted proteins were detected by two-dimensional electrophoresis. dna microarray analysis to identify the transcriptional targets of aoxlnr led to the identification of 75 genes that showed more than fivefold i ...200919777228
uncovering transcriptional regulation of glycerol metabolism in aspergilli through genome-wide gene expression data analysis.glycerol is catabolized by a wide range of microorganisms including aspergillus species. to identify the transcriptional regulation of glycerol metabolism in aspergillus, we analyzed data from triplicate batch fermentations of three different aspergilli (aspergillus nidulans, aspergillus oryzae and aspergillus niger) with glucose and glycerol as carbon sources. protein comparisons and cross-analysis with gene expression data of all three species resulted in the identification of 88 genes having ...200919784673
nonhomologous end-joining deficiency allows large chromosomal deletions to be produced by replacement-type recombination in aspergillus oryzae.gene targeting is a technique of introducing a genetic trait at a predetermined site within a genome; it is also used to eliminate undesirable chromosomal regions from the relevant genome. thus far, replacement-type recombination between two homologous regions separated by a large nonhomologous sequence has been hardly achieved probably due to the low frequency of homologous recombination in filamentous fungi. in this study, we report the successful and highly efficient deletion by replacement-t ...200919654050
isolation of a new metabolite from biotransformation of daidzein by aspergillus oryzae.two compounds were isolated from biotransformation of daidzein, a soybean isoflavone, by aspergillus oryzae. one was 6,7,8,4'-tetrahydroxyisoflavone, an a-ring dihydroxylated daidzein, and the other was a novel compound which had the cleaved b-ring of daidzein. the former was perhaps derived from 8-hydroxydaidzein and/or 6-hydroxydaidzein, and had high dpph radical-scavenging activity.200919661709
genome-wide analysis of maltose utilization and regulation in aspergilli.maltose utilization and regulation in aspergilli is of great importance for cellular physiology and industrial fermentation processes. in aspergillus oryzae, maltose utilization requires a functional mal locus, composed of three genes: malr encoding a regulatory protein, malt encoding maltose permease and mals encoding maltase. through a comparative genome and transcriptome analysis we show that the mal regulon system is active in a. oryzae while it is not present in aspergillus niger. in order ...200919696104
endocytic recycling at the tip region in the filamentous fungus aspergillus oryzae.recent live cell imaging analyzing the components required for endocytosis has elucidated that endocytosis actively occurs at the hyphal tip region in filamentous fungi. to examine further the physiological roles of endocytosis we investigated a conditional mutant of endocytosis in aspergillus oryzae. endocytosis-deficient hyphae displayed retarded apical growth, abnormal hyphal morphology, mislocalization of a vesicle- snare, which is thought to undergo endocytic recycling to the tip region, an ...200919721880
post-genomic insights into the plant polysaccharide degradation potential of aspergillus nidulans and comparison to aspergillus niger and aspergillus oryzae.the plant polysaccharide degradative potential of aspergillus nidulans was analysed in detail and compared to that of aspergillus niger and aspergillus oryzae using a combination of bioinformatics, physiology and transcriptomics. manual verification indicated that 28.4% of the a. nidulans orfs analysed in this study do not contain a secretion signal, of which 40% may be secreted through a non-classical method.while significant differences were found between the species in the numbers of orfs ass ...200919618505
release of antihypertensive peptides in miso paste during its fermentation, by the addition of casein.casein hydrolysate by aspergillus oryzae protease has been developed to obtain two kinds of antihypertensive peptides, val-pro-pro and ile-pro-pro. in this study, casein miso paste was prepared by adding casein at various concentrations during miso paste fermentation in order to release angiotensin-i converting enzyme (ace) inhibitory peptides. increase of peptide concentration and ace inhibitory activity in casein miso paste was observed and corresponded to the degradation of casein and soy bea ...200919619856
cloning of aspergillus oryzae aovps5 gene, homologous to vacuolar protein sorting associated gene vps5 and construction of the disruptant.aovps5 gene was isolated from aspergillus oryzae as a homologue to s. cerevisiae vps5 gene which encodes a polypeptide consisting of 451 amino acids that is nearly 32% homologous to vps5p. three aovps5 gene disruptants were generated and they showed higher activity of tripeptidyl peptidase, which is mainly detected in vacuoles, in their culture medium. higher amount of nitrogenous constituent was found in the filtrate of wheat gluten degraded by addition of culture medium of these disruptants th ...200919619858
a food-grade enzyme preparation with modest gluten detoxification properties.celiac sprue is a life-long disease characterized by an intestinal inflammatory response to dietary gluten. a gluten-free diet is an effective treatment for most patients, but accidental ingestion of gluten is common, leading to incomplete recovery or relapse. food-grade proteases capable of detoxifying moderate quantities of dietary gluten could mitigate this problem.200919621078
identification of a basic helix-loop-helix-type transcription regulator gene in aspergillus oryzae by systematically deleting large chromosomal segments.we previously developed two methods (loop-out and replacement-type recombination) for generating large-scale chromosomal deletions that can be applied to more effective chromosomal engineering in aspergillus oryzae. in this study, the replacement-type method is used to systematically delete large chromosomal dna segments to identify essential and nonessential regions in chromosome 7 (2.93 mb), which is the smallest a. oryzae chromosome and contains a large number of nonsyntenic blocks. we constr ...200919633118
characterization of a neutral ceramidase orthologue from aspergillus oryzae.ceramide is an important molecule not only structurally but also regulationally as a modulator of various cellular events. ceramidase (cdase) are classified into three different types (acid, alkaline, and neutral cdases). neutral cdase could play an important role in the regulation of ceramide levels in the extracellular space. in this study, we describe the characterization of a neutral cdase orthologue from the filamentous fungus aspergillus oryzae. the gene encoding the neutral cdase ortholog ...200919650849
functional expression of the aspergillus flavus pks-nrps hybrid cpaa involved in the biosynthesis of cyclopiazonic acid.alpha-cyclopiazonic acid (cpa) is an indole tetramic acid mycotoxin. based on our identification of the polyketide synthase-nonribosomal peptide synthase (pks-nrps) hybrid gene cpaa involved in cyclopiazonic acid biosynthesis in aspergillus fungi, we carried out heterologous expression of aspergillus flavuscpaa under alpha-amylase promoter in aspergillus oryzae and identified its sole product to be the cpa biosynthetic intermediate cyclo-acetoacetyl-l-tryptophan (caatrp). this result rationalize ...200919410456
pepj is a new extracellular proteinase of aspergillus nidulans.under carbon starvation, aspergillus nidulans released a metallo-proteinase with activities comparable to those of prta, the major extracellular serine proteinase of the fungus. the relative molar mass of the enzyme was 19 kda as determined with both denaturing and renaturing sds page, while its isoelectric point and ph and temperature optima were 8.6, 5.5 and 65 degrees c, respectively. the enzyme was stable at ph 3.5-10.5 and was still active at 95 degrees c in the presence of azocasein substr ...200919418246
purification and identification of proteolytic enzymes from aspergillus oryzae capable of producing the antihypertensive peptide ile-pro-pro.two proteolytic enzymes capable of releasing the angiotensin i-converting enzyme (ace) inhibitor ile-pro-pro from casein were identified by purification of an aspergillus oryzae extract by three-step column chromatography. first, proteins capable of producing ile-pro-pro from beta-casein were eluted using a deae-sepharose ff column with a linear sodium chloride gradient. an endopeptidase capable of releasing pro-ile-pro-gln-ser-leu-pro-gln-asn-ile-pro-pro from pro-ile-pro-gln-ser-leu-pro-gln-asn ...200919447337
an acidophilic beta-galactosidase from bispora sp. mey-1 with high lactose hydrolytic activity under simulated gastric conditions.bgala, a full-length gene (3,009 bp) that encodes a beta-galactosidase, was cloned from the meso-acidophilic fungus bispora sp. mey-1 and expressed in pichia pastoris. the deduced amino acid sequence of bgala shares highest identity (55.5%) with the beta-galactosidase from aspergillus phoenicis, which belongs to the glycoside hydrolyase family 35. purified recombinant bgala is acidophilic, exhibiting maximum activity at ph 1.5, which is lower than that reported for other beta-galactosidases. the ...200919453169
comparison of stirred tank and airlift bioreactors in the production of polygalacturonases by aspergillus oryzae.the production of endo and exo-polygalacturonase (pg) by aspergillus oryzae ipt 301 was studied in a stirred tank bioreactor (str) and an internal circulation airlift bioreactor. using a factorial experimental design, a soluble culture medium was defined which allowed the production of exo- and endo-pg comparable to that obtained in a medium containing suspended wheat bran. the soluble medium was used in tests to compare the production of these enzymes in the str and airlift bioreactor. in these ...200919467860
crystallization of carbohydrate oxidase from microdochium nivale.microdochium nivale carbohydrate oxidase was produced by heterologous recombinant expression in aspergillus oryzae, purified and crystallized. the enzyme crystallizes with varying crystal morphologies depending on the crystallization conditions. several different crystal forms were obtained using the hanging-drop vapour-diffusion method, two of which were used for diffraction measurements. hexagon-shaped crystals (form i) diffracted to 2.66 a resolution, with unit-cell parameters a = b = 55.7, c ...200919478452
alpha-lactalbumin hydrolysate stimulates glucagon-like peptide-2 secretion and small intestinal growth in suckling rats.we investigated whether bovine milk constituents influenced glucagon-like peptide (glp)-2 secretion and intestinal growth in suckling rats. male sprague-dawley rats (14 d old) received i.g. infusions of a milk protein fraction, a lactose solution, or the cream fraction of milk. the serum concentration of glp-2, but not glp-1, markedly increased in rats administered milk protein compared with those given the lactose solution or the cream fraction from 60 to 120 min after administration. in anothe ...200919494023
a unique post-translational processing of an exo-beta-1,3-glucanase of penicillium sp. kh10 expressed in aspergillus oryzae.to characterize an exo-beta-1,3-glucanase (exgp) of an isolated fungal strain with high laminarin degradation activity, identified as penicillium sp. kh10, heterologous secretory expression of the exgp was performed in aspergillus oryzae. deduced amino acid sequence of the exgp gene possibly consisted of 989 amino acids which showed high sequence similarity to those of fungal exo-beta-1,3-glucanases belonging to the glycoside hydrolase (gh) family 55. notably, the purified recombinant exgp showe ...200919500674
is electron equivalence between substrate and product preferable to c-mol equivalence in representations of microbial anabolism applicable to "origin of life" environmental conditions.this research compares two techniques for constructing sets of equations representing the growth of a microorganism on a given substrate. each anabolic equation represents a defined initial and a final state. the initial state comprises all necessary mineral nutrients and one organic source of carbon and energy. the final state comprises the microbial cells that have grown, and any additional organic or inorganic products of the growth process. the cells are represented by a unit-carbon formula ...200919501106
functional analysis and subcellular localization of two geranylgeranyl diphosphate synthases from penicillium paxilli.the filamentous fungus penicillium paxilli contains two distinct geranylgeranyl diphosphate (ggpp) synthases, ggsa and ggsb (paxg). paxg and its homologues in neotyphodium lolii and fusarium fujikuroi are associated with diterpene secondary metabolite gene clusters. the genomes of other filamentous fungi including aspergillus fumigatus, aspergillus nidulans, aspergillus niger, aspergillus oryzae and fusarium graminearum also contain two or more copies of ggpp synthase genes, although the diterpe ...200919529962
cereal-based biorefinery development: utilisation of wheat milling by-products for the production of succinic acid.a novel wheat-based bioprocess for the production of a nutrient-complete feedstock for the fermentative succinic acid production by actinobacillus succinogenes has been developed. wheat was fractionated into bran, middlings and flour. the bran fraction, which would normally be a waste product of the wheat milling industry, was used as the sole medium in two solid-state fermentations (ssf) of aspergillus awamori and aspergillus oryzae that produce enzyme complexes rich in amylolytic and proteolyt ...200919539669
modified electrophoretic and digestion conditions allow a simplified mass spectrometric evaluation of disulfide bonds.proper formation of disulfide bonds in proteins is a prerequisite to their stability and function. information on disulfide pattern may therefore serve as an indication of the proper folding of recombinant proteins, and can also be used in protein homology modeling for the purpose of structure refinement. protein handling and digestion at basic ph leads to disulfide bond scrambling. that is why the samples are usually treated and digested at low ph where no scrambling occurs. unfortunately, the ...200919551705
heterologous expression and characterization of cpi, ocpa, and novel serine-type carboxypeptidase ocpb from aspergillus oryzae.in the genome of aspergillus oryzae, 12 genes have been predicted to encode serine-type carboxypeptidases. however, the carboxypeptidase activities of the proteins encoded by these genes have not yet been confirmed experimentally. in this study, we have constructed three of these 12 genes overexpressing strains using aspergillus nidulans and characterized their overproduced recombinant proteins. of these three genes, one was previously named cpi; the other two have not been reported yet, and hen ...200919557408
responses of aspergillus oryzae to stimuli from near-uv light irradiation in the presence or absence of titanium dioxide.the responses of aspergillus oryzae to the stimuli from near-uv irradiation were investigated by using a black light fluorescent lamp in the presence or absence of tio2 particles. light irradiation at an intensity of 6 w/m2 strongly inhibited the growth of germinated pellets of a. oryzae. this growth inhibition was weakened by tio2 particles (0.05 g/l), especially in the initial growth phase, in which the expression level of catalase gene (catb) was approximately three times higher than that in ...200919579656
[promotive excretion of causative agents of yusho by intake of fermented brown rice with aspergillus oryze in patients with yusho --with regard to pcdfs and pcdds].forty years have passed since the outbreak of kanemi rice oil poisoning, namely, yusho in the western japan. however, even now the patients with yusho have been still suffering from several objective and subjective symptoms. in order to improve or, if possible, to cure such symptoms, the most important therapeutic treatment is considered to actively excrete the causative agents, that is, polychlorinated dibenzofurans (pcdfs) and polychlorinated dibenzo-p-dioxins (pcdds) from the bodies of the pa ...200919588849
improved production of raw starch degrading enzyme by aspergillus oryzae f-30 using methyl glucoside sesqui-stearate.the effect of methyl glucoside sesqui-stearate (mgs) on the production of raw starch degrading enzyme (rsde) by aspergillus oryzae f-30 was studied in this paper. the activity of rsde formed by aspergillus oryzae f-30 was enhanced dramatically by the addition of mgs to the medium. as a result, with the addition of 1.5 g mgs in 1 l basal medium, rsde activity and productivity were 107 u ml(-1) and 1.49 u ml(-1) h(-1), 4.3-fold and 7.1-fold greater than the values obtained in the basal medium, res ...200919002609
endocytosis is crucial for cell polarity and apical membrane recycling in the filamentous fungus aspergillus oryzae.establishing the occurrence of endocytosis in filamentous fungi was elusive in the past mainly due to the lack of reliable indicators of endocytosis. recently, however, it was shown that the fluorescent dye n-(3-triethylammoniumpropyl)-4-(p-diethyl-aminophenyl-hexatrienyl)pyridinium dibromide (fm4-64) and the plasma membrane protein aouapc (aspergillus oryzae uapc) fused to enhanced green fluorescent protein (egfp) were internalized from the plasma membrane by endocytosis. although the occurrenc ...200919028995
metabolism of hydroxylated pcb congeners by cloned laccase isoforms.the white-rot fungus t. versicolor uamh 8272 produced two groups of laccases, each of which included several isoforms showing different isoelectric points (pi). group 1 and group 2 laccases, respectively, displayed higher pi 5-6 and lower pi 3-4. of the four cloned full-length laccase cdnas, lac 1 and lac 4 were expressed in the heterologous protein expression system using aspergillus oryzae. the measured pi of each lac 1 and lac 4 expressed in a. oryzae was lower than that of pi predicted from ...200919066882
characterization of two distinct feruloyl esterases, aofaeb and aofaec, from aspergillus oryzae.two hypothetical proteins xp_001818628 and xp_001819091 (designated aofaeb and aofaec, respectively), showing sequence identity with known type-c feruloyl esterases, have been found in the genomic sequence of aspergillus oryzae. we cloned the putative a. oryzae feruloyl esterase-encoding genes and expressed them in pichia pastoris. both purified recombinant aofaeb (raofaeb) and aofaec (raofaec) had apparent relative molecular masses of 61,000 and 75,000, respectively, on sodium dodecyl sulfate-p ...200919242690
crystal structure of a fungal protease inhibitor from antheraea mylitta.indian tasar silk is produced by a wild insect called antheraea mylitta. insects do not have any antigen-antibody mediated immune system like vertebrates but they produce a wide variety of effector proteins and peptides possessing potent antifungal and antibacterial activity to combat microbial attack. antheraea mylitta expresses a fungal protease inhibitor amfpi-1, in the hemolymph that inhibits alkaline protease of aspergillus oryzae for protection against fungal infection. amfpi-1 is purified ...200919263521
crawler, a novel tc1/mariner-type transposable element in aspergillus oryzae transposes under stress conditions.a novel active transposable element, designated crawler, has been isolated from an industrial strain (osi1013) of aspergillus oryzae as an insertion sequence within the niad gene encoding nitrate reductase. it is 1290bp in length with imperfect terminal inverted repeats of 28bp and is flanked by 2bp (ta) target site duplications. it contains an open reading frame with no introns that encodes a putative transposase (aota) of 357 amino acid residues, which is highly homologous to the transposase e ...200919269345
morphological quantification of filamentous fungal development using membrane immobilization and automatic image analysis.mycelial morphology is a critically important process property in industrial fermentations of filamentous micro-organisms, as particular phenotypes are associated with maximum productivity. however, the accurate quantification of complex morphologies still represents a significant challenge in elucidating this relationship. a system has been developed for high-resolution characterisation of filamentous fungal growth on a solid substrate, using membrane immobilization and fully-automatic plug-ins ...200919277741
a novel transformation system using a bleomycin resistance marker with chemosensitizers for aspergillus oryzae.aspergillus oryzae is resistant to many kinds of antibiotics, which hampers their use to select transformants. in fact, the fungus is resistant to over 200microg/ml of bleomycin (bm). by enhancing the susceptibility of a. oryzae to bm using triton x-100 as a detergent and an atp-binding cassette (abc) pump inhibitor, chlorpromazine, to the growing medium, we established a novel transformation system by bm selection for a. oryzae. in a medium containing these reagents, a. oryzae showed little gro ...200919324021
analysis of microbial communities in doenjang, a korean fermented soybean paste, using nested pcr-denaturing gradient gel electrophoresis.doenjang is a traditional korean fermented soybean paste that provides a major source of protein. the microbial diversity of 10 samples of doenjang (5 commercially manufactured products and 5 homemade products) was investigated using nested pcr-denaturing gradient gel electrophoresis (dgge). in the first step, the nearly complete 16s rrna and 18s rrna genes were amplified using universal primers. subsequently, these products were used as a template in a nested pcr to obtain fragments suitable fo ...200919324443
deletion analysis of the promoter of aspergillus oryzae gene encoding heat shock protein 30.in order to find a promoter that could be influenced by temperature shift, we explored and isolated an aspergillus oryzae gene expressed at high temperatures (37-42 degrees c) by the cdna subtraction method. of the 96 cdna clones isolated from the subtraction library, one cdna clone showed 73% identity with aspergillus nidulans heat shock protein 30 (hsp30). based on this, we designated the isolated gene hsp30 of a. oryzae. a. oryzae hsp30 was weakly expressed at 30 degrees c, but strongly at 40 ...200919332290
development of biosensors containing laccase and imidazolium bis(trifluoromethylsulfonyl)imide ionic liquid for the determination of rutin.biosensors based on hydrophobic ionic liquids (ils) derived from the bis(trifluoromethylsulfonyl)imide [(cf(3)so(2))(2)n(-) = tf(2)n(-)] anion associated with three different imidazolium cations: 1-butyl-3-methylimidazolium (bmi x tf(2)n), 1-decyl-3-methylimidazolium (dmi x tf(2)n) and 1-tetradecyl-3-methylimidazolium (tdmi x tf(2)n), along with laccase from aspergillus oryzae, were constructed and optimized for determination of rutin. the laccase catalyzes the oxidation of rutin to the correspo ...200919345764
cloning and characterization of two flavohemoglobins from aspergillus oryzae.two flavohemoglobin (fhb) genes, fhb1 and fhb2, were cloned from aspergillus oryzae. the amino acid sequences of the deduced fhb1 and fhb2 showed high identity to other fhbs except for the predicted mitochondrial targeting signal in the n-terminus of fhb2. the recombinant proteins displayed absorption spectra similar to those of other fhbs. fhb1 and fhb2 were estimated to be a monomer and a dimer in solution, respectively. both of the isozymes exhibit high no dioxygenase (nod) activity. fhb1 uti ...200919351585
atan1p-an extracellular tannase from the dimorphic yeast arxula adeninivorans: molecular cloning of the atan1 gene and characterization of the recombinant enzyme.the tannase-encoding arxula adeninivorans gene atan1 was isolated from genomic dna by pcr, using as primers oligonucleotide sequences derived from peptides obtained after tryptic digestion of the purified tannase protein. the gene harbours an orf of 1764 bp, encoding a 587-amino acid protein, preceded by an n-terminal secretion sequence comprising 28 residues. the deduced amino acid sequence was similar to those of tannases from aspergillus oryzae (50% identity), a. niger (48%) and putative tann ...200919387973
evaluating the potential for enzymatic acrylamide mitigation in a range of food products using an asparaginase from aspergillus oryzae.asparaginase, an enzyme that hydrolyzes asparagine to aspartic acid, presents a potentially very effective means for reducing acrylamide formation in foods via removal of the precursor, asparagine, from the primary ingredients. an extracellular asparaginase amenable to industrial production was cloned and expressed in aspergillus oryzae . this asparaginase was tested in a range of food products, including semisweet biscuits, ginger biscuits, crisp bread, french fries, and sliced potato chips. in ...200919388639
a biotechnological product and its potential as a new immunomodulator for treatment of animal phlebovirus infection: punta toro virus.intracellular pathogens with widespread drug-resistance contribute substantially to the increasing rates in morbidity and mortality due to emerging and reemerging diseases. thus, the development of new drugs, including those that can enhance the immune response, is urgently needed. the immunomodulator, p-mapa, a proteinaceous aggregate of ammonium and magnesium phospholinoleate-palmitoleate anhydride derived from aspergillus oryzae, have been shown to induce antitumor activities. the ability of ...200919393266
genetic analysis of glucosidase ii beta-subunit in trimming of high-mannose-type glycans.glucosidase ii (g-ii) is a glycoprotein-processing enzyme that successively cleaves two alpha1,3-linked glucose residues from n-linked oligosaccharides in the endoplasmic reticulum. g-ii is a heterodimer whose alpha-subunit contains a glycosidase active site, but the function(s) of the beta-subunit remain poorly defined. we report here an in vivo enzymatic analysis using gene disruptants lacking either the g-ii alpha- or beta-subunit in the filamentous fungus aspergillus oryzae. using synthetic ...200919395677
expression in escherichia coli of an unnamed protein gene from aspergillus oryzae rib40 and cofactor analyses of the gene product as formate oxidase.an unnamed protein of aspergillus oryzae rib40 (accession no. xp_001727378), the amino acid sequence of which shows high similarity to those of formate oxidase isoforms produced by debaryomyces vanjiriae mh201, was produced in escherichia coli in c-his(6)-tagged form. the gene product, purified by affinity column chromatography, catalyzed the oxidation of formate to yield hydrogen peroxide but showed no evidence of activity on the other substrates tested. the k(m) and v(max) values at 30 degrees ...200919966484
simultaneous preparation of purified plasmalogens and sphingomyelin in human erythrocytes with phospholipase a1 from aspergillus orizae.a method for the simultaneous purification of plasmalogens and sphingomyelin (sm) in human erythrocytes is described. treatment of total lipids with n-hexane/acetone (1:1 v/v) resulted in selective precipitation of sm. both the supernatant and the precipitate fractions were incubated with a phospholipase a(1) (pla1) from aspergillus orizae for 3.5 h. the pla1-treated lipids were extracted with n-hexane/isopropanol, the hexane layer was obtained using a na(2)so(4) solution, and the hexane layer w ...200919966491
characterization of cyclo-acetoacetyl-l-tryptophan dimethylallyltransferase in cyclopiazonic acid biosynthesis: substrate promiscuity and site directed mutagenesis studies.the fungal neurotoxin alpha-cyclopiazonic acid (cpa), a nanomolar inhibitor of ca(2+)-atpase with a unique pentacyclic indole tetramic acid scaffold, is assembled by a three enzyme pathway cpas, cpad, and cpao in aspergillus sp. we recently characterized the first pathway-specific enzyme cpas, a hybrid two module polyketide synthase-nonribosomal peptide synthetase (pks-nrps) that generates cyclo-acetoacetyl-l-tryptophan (caatrp). here we report the characterization of the second pathway-specific ...200919877600
a cytosolic phospholipase a2-like protein in the filamentous fungus aspergillus oryzae localizes to the intramembrane space of the mitochondria.in mammalian cells, cytosolic phospholipase a(2) (cpla(2)) displays a variety of activities through the release of arachidonic acid in response to cellular stimuli. in this study, we characterize the putative cpla(2)-like protein, aoplaa, in the filamentous fungus aspergillus oryzae. when aoplaa-egfp was expressed in a. oryzae, it localized to the tubular structures that was costained by the marker dye for the mitochondria. a biochemical fractionation experiment showed that aoplaa was present in ...200919889028
characterization of galactosyl derivatives obtained by transgalactosylation of lactose and different polyols using immobilized beta-galactosidase from aspergillus oryzae.the synthesis of novel galactosides is interesting because of their important role in several biological processes. their properties greatly depend upon the configuration and type of galactoside. therefore, to study biological activity, it is essential to elucidate the structure of the products. glycosidases are capable of catalyzing glycosidic linkages with absolute stereoselectivity of the anomeric center. we report the enzymatic synthesis of galactosyl-ethylene glycol, galactosyl-glycerol, an ...200919891502
improvement of heterologous protein production in aspergillus oryzae by rna interference with alpha-amylase genes.aspergillus oryzae rib40 has three alpha-amylase genes (amya, amyb, and amyc), and secretes alpha-amylase abundantly. however, large amounts of endogenous secretory proteins such as alpha-amylase can compete with heterologous protein in the secretory pathway and decrease its production yields. in this study, we examined the effects of suppression of alpha-amylase on heterologous protein production in a. oryzae, using the bovine chymosin (chy) as a reporter heterologous protein. the three alpha-a ...200919897917
using a new gpi-anchored-protein identification system to mine the protein databases of aspergillus fumigatus, aspergillus nidulans, and aspergillus oryzae.computational approaches provide valuable information to start experimental surveys identifying glycosylphosphatidylinositol (gpi)-anchored proteins in protein sequence databases. we developed a new sequence-based identification system that uses an optimized classifier based on a support vector machine (svm) algorithm to recognize appropriate cooh-terminal sequences and uses a classifier implementing a simple majority voting strategy to recognize appropriate nh2-terminal sequences. the svm class ...200919940384
annotation of stress-response proteins in the aspergilli.stress-response proteins of aspergillus nidulans, aspergillus clavatus, aspergillus flavus, aspergillus fumigatus, aspergillus niger, aspergillus oryzae, aspergillus terreus and neosartorya fischeri (3908 in total) were annotated and grouped according to stress types (http://193.6.155.82/aspergillusstress/). all genomes harboured elements of the sska-hoga/saka stress signalling pathway. there are accumulating data pointing at the importance of sska-hoga/saka signalling in different types of stre ...200918703157
value addition to corncob: production and characterization of xylooligosaccharides from alkali pretreated lignin-saccharide complex using aspergillus oryzae mtcc 5154.comparison of various pretreatments such as mild alkali/acid treatments and pressure cooking of corncob to expose its lignin-saccharide complex has been carried out to enhance enzymatic hydrolysis of xylan to xylooligosaccharides (xos). scanning electron micrographs of lignin-saccharide complex of native and pretreated corncob powder showed that the complex was greatly altered during alkali pretreatment. hydrolysis of alkali pretreated corncob powder using a commercial endoxylanase produced 81+/ ...200918703333
catalytic and thermodynamic characterization of endoglucanase (cmcase) from aspergillus oryzae cmc-1.monomeric extracellular endoglucanase (25 kda) of transgenic koji (aspergillus oryzae cmc-1) produced under submerged growth condition (7.5 u mg(-1) protein) was purified to homogeneity level by ammonium sulfate precipitation and various column chromatography on fast protein liquid chromatography system. activation energy for carboxymethylcellulose (cmc) hydrolysis was 3.32 kj mol(-1) at optimum temperature (55 degrees c), and its temperature quotient (q (10)) was 1.0. the enzyme was stable over ...200918704277
enzymatic removal of flatulence-inducing sugars in chickpea milk using free and polyvinyl alcohol immobilized alpha-galactosidase from aspergillus oryzae.the treatment of chickpea milk was carried out in batch, repeated batch and continuous reaction by soluble and polyvinyl alcohol (pva) immobilized aspergillus oryzae alpha-galactosidase for the removal of raffinose family oligosaccharides (rfos). in the batch mode of treatment 96 and 92% of rfos hydrolysis was observed by soluble and immobilized enzyme, respectively. in repeated batch experiments, immobilized enzyme showed 70% rfos hydrolysis up to sixth cycle. polyvinyl alcohol immobilized alph ...200918839231
three-phase partitioning of alpha-galactosidase from fermented media of aspergillus oryzae and comparison with conventional purification techniques.simple, attractive and versatile technique, three-phase partitioning (tpp) was used to purify alpha-galactosidase from fermented media of aspergillus oryzae. the various conditions required for attaining efficient purification of the alpha-galactosidase fractions were optimized. the addition of n-butanol, t-butanol, and isopropanol in the presence of ammonium sulfate pushes the protein out of the solution to form an interfacial precipitate layer between the lower aqueous and upper organic layers ...200918846399
analysis and prediction of gene splice sites in four aspergillus genomes.several aspergillus fungal genomic sequences have been published, with many more in progress. obviously, it is essential to have high-quality, consistently annotated sets of proteins from each of the genomes, in order to make meaningful comparisons. we have developed a dedicated, publicly available, splice site prediction program called netaspgene, for the genus aspergillus. gene sequences from aspergillus fumigatus, the most common mould pathogen, were used to build and test our model. compared ...200918948220
bioremediation of dyes in textile effluents by aspergillus oryzae.in this study aspergillus oryzae was utilized to remove azo dyes from aqueous solution. physically induced in its paramorphogenic form to produce standardized mycelial pellets, the non-autoclaved and autoclaved hyphae biomass was applied to biosorb the reactive dyes procion red he7b (pr-he7b) and procion violet h3r (pv-h3r) at different ph values (2.50, 4.50, and 6.50). the best ph for biosorption was 2.50, though the autoclaved demonstrated a higher biosorption capacity than the non-autoclaved ...200918989608
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