TitleAbstractYear(sorted descending)
fucosylation is associated with the malignant transformation of intraductal papillary mucinous neoplasms: a lectin microarray-based study.intraductal papillary mucinous neoplasm (ipmn) is an intraductal mucin-producing pancreatic neoplasm with the potential for malignant transformation. changes in glycans expressed on the cell surface and glycotransferases play important roles in malignant transformation. we conducted this study to analyze glycan alterations in ipmns by using a lectin microarray and to identify the factors associated with altered glycans and their relationships with malignant transformation.201626754572
illuminating the binding interactions of galactonoamidines during the inhibition of β-galactosidase (e. coli).several galactonoamidines were previously identified as very potent competitive inhibitors that exhibit stabilizing hydrophobic interactions of the aglycon in the active site of β-galactosidase (aspergillus oryzae). to elucidate the contributions of the glycon to the overall inhibition ability of the compounds, three glyconoamidine derivatives with alteration in the glycon at c-2 and c-4 were synthesized and evaluated herein. all amidines are competitive inhibitors of β-galactosidase (escherichi ...201626740154
evaluation of kojic acid production in a repeated-batch pcs biofilm this study, kojic acid, a secondary metabolite as an industrially important compound, was produced by aspergillus oryzae (a. oryzae), which was immobilized in plastic composite support (pcs) bioreactor. nitrogen deficient medium was applied to increase the production of ka in pcs-immobilized bioreactor. the efficiency of immobilized culture for kojic acid (ka) production and the effect of morphology of a. oryzae on ka production were evaluated. after three cycles of cultivation, 83.47 g/l of ...201626657710
toward rational thermostabilization of aspergillus oryzae cutinase: insights into catalytic and structural stability.cutinases are powerful hydrolases that can cleave ester bonds of polyesters such as poly(ethylene terephthalate) (pet), opening up new options for enzymatic routes for polymer recycling and surface modification reactions. cutinase from aspergillus oryzae (aoc) is promising owing to the presence of an extended groove near the catalytic triad which is important for the orientation of polymeric chains. however, the catalytic efficiency of aoc on rigid polymers like pet is limited by its low thermos ...201626522152
identification of the gene encoding isoprimeverose-producing oligoxyloglucan hydrolase in aspergillus oryzae.aspergillus oryzae produces a unique β-glucosidase, isoprimeverose-producing oligoxyloglucan hydrolase (ipase), that recognizes and releases isoprimeverose (α-d-xylopyranose-(1 → 6)-d-glucopyranose) units from the non-reducing ends of oligoxyloglucans. a gene encoding a. oryzae ipase, termed ipea, was identified and expressed in pichia pastoris. with the exception of cellobiose, ipea hydrolyzes a variety of oligoxyloglucans and is a member of the glycoside hydrolase family 3. xylopyranosyl branc ...201626755723
further increased production of free fatty acids by overexpressing a predicted transketolase gene of the pentose phosphate pathway in aspergillus oryzae faaa fatty acids are useful as source materials for the production of biodiesel fuel and various chemicals such as pharmaceuticals and dietary supplements. previously, we attained a 9.2-fold increase in free fatty acid productivity by disrupting a predicted acyl-coa synthetase gene (faaa, ao090011000642) in aspergillus oryzae. in this study, we achieved further increase in the productivity by overexpressing a predicted transketolase gene of the pentose phosphate pathway in the faaa disruptant. t ...201626998626
substantial decrease in cell wall α-1,3-glucan caused by disruption of the kexb gene encoding a subtilisin-like processing protease in aspergillus oryzae.disruption of the kexb encoding a subtilisin-like processing protease in aspergillus oryzae (δkexb) leads to substantial morphological defects when the cells are grown on czapek-dox agar plates. we previously found that the disruption of kexb causes a constitutive activation of the cell wall integrity pathway. to understand how the disruption of the kexb affects cell wall organization and components, we analyzed the cell wall of δkexb grown on the plates. the results revealed that both total n-a ...201626980104
the c2h2-type transcription factor, flbc, is involved in the transcriptional regulation of aspergillus oryzae glucoamylase and protease genes specifically expressed in solid-state culture.aspergillus oryzae produces a large amount of secreted proteins in solid-state culture, and some proteins such as glucoamylase (glab) and acid protease (pepa) are specifically produced in solid-state culture, but rarely in submerged culture. from the disruption mutant library of a. oryzae transcriptional regulators, we successfully identified a disruption mutant showing an extremely low production level of glab but a normal level of α-amylase production. this strain was a disruption mutant of th ...201626960315
production of 3-oxo-2-(2'-pentenyl)-cyclopentane-1-octanoic acid in the fungus aspergillus oryzae: a step towards heterologous production of pyrethrins in fungi.pyrethrins are natural insecticides, which accumulate to high concentrations in pyrethrum (chrysanthemum cinerariaefolium) flowers. synthetic pyrethroids are more stable, more efficacious and cheaper, but contemporary requirements for safe and environmentally friendly pesticides encourage a return to the use of natural pyrethrins, and this would be favoured by development of an efficient route to their production by microbial fermentation. the biosynthesis of pyrethrins involves ester linkage be ...201626718544
improving 3'-hydroxygenistein production in recombinant pichia pastoris using periodic hydrogen peroxide-shocking strategy.3'-hydroxygenistein can be obtained from the biotransformation of genistein by the engineered pichia pastoris x-33 strain, which harbors a fusion gene composed of cyp57b3 from aspergillus oryzae and a cytochrome p450 oxidoreductase gene (scpr) from saccharomyces cerevisiae. p. pastoris x-33 mutants with higher 3'-hydroxygenistein production were selected using a periodic hydrogen peroxide-shocking strategy. one mutant (p2-d14-5) produced 23.0 mg/l of 3'-hydroxygenistein, representing 1.87-fold m ...201626699751
autolysis of aspergillus oryzae mycelium and effect on volatile flavor compounds of soy sauce.the autolyzed mycelia of aspergillus oryzae are rich in proteins, nucleic acids, sugar, and other biomacromolecules, and are one of the main contributors to the flavor profile of commercially important fermented goods, including soy sauce and miso. we induced autolysis of the mycelia of a. oryzae over 1 to 10 d, and found that the maximum dissolved amounts of total protein and nucleic acid ratio accounted for 28.63% and 88.93%, respectively. the organic acid content, such as citric acid, tartari ...201627464006
sequential mixed cultures: from syngas to malic acid.synthesis gas (syngas) fermentation using acetogenic bacteria is an approach for production of bulk chemicals like acetate, ethanol, butanol, or 2,3-butandiol avoiding the fuel vs. food debate by using carbon monoxide, carbon dioxide, and hydrogen from gasification of biomass or industrial waste gases. suffering from energetic limitations, yields of c4-molecules produced by syngas fermentation are quite low compared with abe fermentation using sugars as a substrate. on the other hand, fungal pro ...201627445993
comparative transcriptome analysis revealing dormant conidia and germination associated genes in aspergillus species: an essential role for atfa in conidial dormancy.fungal conidia are usually dormant unless the extracellular conditions are right for germination. despite the importance of dormancy, little is known about the molecular mechanism underlying entry to, maintenance of, and exit from dormancy. to gain comprehensive and inter-species insights, transcriptome analyses were conducted across aspergillus fumigatus, aspergillus niger, and aspergillus oryzae.201627185182
multi-response optimisation of the extraction solvent system for phenolics and antioxidant activities from fermented soy flour using a simplex-centroid design.a simplex-centroid design comprising three solvents (water, ethanol and methanol) was used to optimise the extraction mixture for phenolics and antioxidant activities from defatted soy flour fermented with monascus purpureus or aspergillus oryzae. total phenolics were more efficiently extracted using only water for both samples. the highest antioxidant activities by the dpph and abts methods were obtained using extraction mixtures containing at least 75 wt% water. specific water:ethanol:methanol ...201626616938
novel 4-methyl-2-oxopentanoate reductase involved in synthesis of the japanese sake flavor, ethyl leucate.ethyl-2-hydroxy-4-methylpentanoate (ethyl leucate) contributes to a fruity flavor in japanese sake. the mold aspergillus oryzae synthesizes leucate from leucine and then the yeast saccharomyces cerevisiae produces ethyl leucate from leucate during sake fermentation. here, we investigated the enzyme involved in leucate synthesis by a. oryzae. the a. oryzae gene/cdna encoding the enzyme involved in leucate synthesis was identified and expressed in e. coli and a. oryzae host cells. the purified rec ...201626615399
highly efficient fructooligosaccharides production by an erythritol-producing yeast yarrowia lipolytica displaying fructosyltransferase.currently, fructooligosaccharides (fos) are industrially transformed from sucrose by purified enzymes or fungi cells. however, these methods are expensive and time-consuming. an economical approach to producing fos using erythritol-producing yeast cells was described in this study. fructosyltransferase from aspergillus oryzae was displayed on the cell surface of yarrowia lipolytica, resulting in an engineered strain capable of transforming sucrose to fos. an amount of 480 g/l fos was produced wi ...201627124471
comparison of catalytic properties of multiple β-glucosidases of trichoderma reesei.ten putative trichoderma reesei β-glucosidase (bgl) isozymes were heterologously expressed in escherichia coli and aspergillus oryzae and purified to homogeneity. catalytic properties of nine enzymes which showed hydrolytic activity on cellobiose and p-nitrophenyl-β-d-glucopyranoside (pnpg) were investigated. three bgls, encoded by the genes cel3a, cel3b, and cel3e, contained a predicted signal peptide, showed higher hydrolytic activity on cello-oligosaccharides than on pnpg, and preferred longe ...201626846743
chitosan crosslinked with genipin as support matrix for application in food process: support characterization and β-d-galactosidase order to develop safer processes for the food industry, we prepared a chitosan support with the naturally occurring crosslinking reagent, genipin, for enzyme. as application model, it was tested for the immobilization of β-d-galactosidase from aspergillus oryzae. chitosan particles were obtained by precipitation followed by adsorption of the enzyme and crosslinking with genipin. the particles were characterized by fourier transform infrared (ftir) spectroscopy and thermogravimetric analysis ( ...201626686119
recombinant expression, purification, and characterization of an acyl-coa binding protein from aspergillus characterize biochemically the lipid metabolism-regulating acyl-coa binding protein (acbp) from the industrially-important fungus aspergillus oryzae.201626677124
optimization of liquid-state fermentation conditions for the glyphosate degradation enzyme production of strain aspergillus oryzae by ultraviolet mutagenesis.this study aimed to obtain strains with high glyphosate-degrading ability and improve the ability of glyphosate degradation enzyme by the optimization of fermentation conditions. spore from aspergillus oryzae a-f02 was subjected to ultraviolet mutagenesis. single-factor experiment and response surface methodology were used to optimize glyphosate degradation enzyme production from mutant strain by liquid-state fermentation. four mutant strains were obtained and named as fujx 001, fujx 002, fujx 0 ...201626795747
an unusual chimeric diterpene synthase from emericella variecolor and its functional conversion into a sesterterpene synthase by domain swapping.di- and sesterterpene synthases produce c20 and c25 isoprenoid scaffolds from geranylgeranyl pyrophosphate (ggpp) and geranylfarnesyl pyrophosphate (gfpp), respectively. by genome mining of the fungus emericella variecolor, we identified a multitasking chimeric terpene synthase, evvs, which has terpene cyclase (tc) and prenyltransferase (pt) domains. heterologous gene expression in aspergillus oryzae led to the isolation of variediene (1), a novel tricyclic diterpene hydrocarbon. intriguingly, i ...201626546087
reconstitution of biosynthetic machinery of fungal polyketides: unexpected oxidations of biosynthetic intermediates by expression host.reconstitution of whole biosynthetic genes in aspergillus oryzae has successfully applied for total biosynthesis of various fungal natural products. heterologous production of fungal metabolites sometimes suffers unexpected side reactions by host enzymes. in the studies on fungal polyketides solanapyrone and cytochalasin, unexpected oxidations of terminal olefin of biosynthetic intermediates were found to give one and four by-products by host enzymes of the transformants harboring biosynthetic g ...201626539980
enzymatically hydrolysed, acetylated and dually modified corn starch: physico-chemical, rheological and nutritional properties and effects on cake quality.corn starch was treated by enzymatic hydrolysis with aspergillus oryzae s2 α-amylase, acetylation with vinyl acetate, and dual modification. the dual modified starch displayed a higher substitution degree than the acetylated starch and lower reducing sugar content than the hydrolysed starch. the results revealed that the cooling viscosity and amylose content of those products decrease (p < 0.05). an increase in moisture, water, and oil absorption capacity was observed for the acetylated starch a ...201626787967
fermented brown rice extract causes apoptotic death of human acute lymphoblastic leukemia cells via death receptor pathway.mixture of brown rice and rice bran fermented with aspergillus oryzae, designated as fbra, has been reported to reveal anti-carcinogenic and anti-inflammatory effects in rodents. then, to test its potential anti-cancer activity, the aqueous extract was prepared from fbra powder, and the effect of this extract on human acute lymphoblastic leukemia jurkat cells was directly examined. the exposure to fbra extract reduced the cell viability in a concentration- and time-dependent manner. the reductio ...201626769704
aberrant glycosylation in the left ventricle and plasma of rats with cardiac hypertrophy and heart failure.targeted proteomics focusing on post-translational modifications, including glycosylation, is a useful strategy for discovering novel biomarkers. to apply this strategy effectively to cardiac hypertrophy and resultant heart failure, we aimed to characterize glycosylation profiles in the left ventricle and plasma of rats with cardiac hypertrophy. dahl salt-sensitive hypertensive rats, a model of hypertension-induced cardiac hypertrophy, were fed a high-salt (8% nacl) diet starting at 6 weeks. as ...201627281159
development of a promoter shutoff system in aspergillus oryzae using a sorbitol-sensitive promoter.promoter shutoff is a general method for analyzing essential genes, but in the fungus aspergillus oryzae, no tightly repressed promoters have been reported. to overcome the current limitations of conditional promoters, we examined sorbitol- and galactose-responsive genes using microarrays to identify regulatable genes with only minor physiological and genetic effects. we identified two sorbitol-induced genes (designated as sora and sorb), cloned their promoters, and built a regulated egfp and br ...201627280333
sequence- and structure-based functional annotation and assessment of metabolic transporters in aspergillus oryzae: a representative case study.aspergillus oryzae is widely used for the industrial production of enzymes. in a. oryzae metabolism, transporters appear to play crucial roles in controlling the flux of molecules for energy generation, nutrients delivery, and waste elimination in the cell. while the a. oryzae genome sequence is available, transporter annotation remains limited and thus the connectivity of metabolic networks is incomplete. in this study, we developed a metabolic annotation strategy to understand the relationship ...201627274991
production of alpha-amylase from aspergillus oryzae for several industrial applications in a single step.a one-step method as a strategy of alpha-amylase concentration and purification was developed in this work. this methodology requires the use of a very low concentration of biodegradable polyelectrolyte (eudragit(®) e-po) and represents a low cost, fast, easy to scale up and non-polluting technology. besides, this methodology allows recycling the polymer after precipitation. the formation of reversible soluble/insoluble complexes between alpha-amylase and the polymer eudragit(®) e-po was studied ...201627085017
biotransformation of l-tyrosine to dopamine by a calcium alginate immobilized mutant strain of aspergillus oryzae.the present research work is concerned with the biotransformation of l-tyrosine to dopamine (da) by calcium alginate entrapped conidiospores of a mutant strain of aspergillus oryzae. different strains of a. oryzae were isolated from soil. out of 13 isolated strains, isolate-2 (i-2) was found to be a better da producer. the wild-type i-2 was chemically improved by treating it with different concentrations of ethyl methyl sulfonate (ems). among seven mutant variants, ems-6 exhibiting maximal da ac ...201627068833
modeling lactose hydrolysis for efficiency and selectivity: toward the preservation of sialyloligosaccharides in bovine colostrum whey permeate.enzymatic hydrolysis of lactose has been shown to improve the efficiency and selectivity of membrane-based separations toward the recovery of bioactive oligosaccharides. achieving maximum lactose hydrolysis requires intrinsic process optimization for each specific substrate, but the effects of those processing conditions on the target oligosaccharides are not well understood. response surface methodology was used to investigate the effects of ph (3.25-8.25), temperature (35-55°c), reaction time ...201627236766
nematicidal activity of kojic acid produced by aspergillus oryzae against meloidogyne incognita.the fungal strain eml-dml3pna1 isolated from leaf of white dogwood (cornus alba l.) showed strong nematicidal activity with juvenile mortality of 87.6% at a concentration of 20% fermentation broth filtrate at 3 days after treatment. the active fungal strain was identified as aspergillus oryzae, which belongs to section flavi, based on the morphological characteristics and sequence analysis of the its rdna, calmodulin (cam), and β-tubulin (bena) genes. the strain reduced the ph value to 5.62 afte ...201627197670
aspergillus oryzae s2 α-amylase domain c involvement in activity and specificity: in vivo proteolysis, molecular and docking studies.we previously reported that aspergillus oryzae strain s2 had produced two α-amylase isoforms named amya and amyb. the apparent molecular masses revealed by sds-page were 50 and 42 kda, respectively. yet amyb has a higher catalytic efficiency. based on a monitoring study of the α-amylase production in both the presence and absence of different protease inhibitors, a chymotrypsin proteolysis process was detected in vivo generating amyb. a. oryzae s2 α-amylase gene was amplified, cloned and sequenc ...201627101008
an alternative approach to synthesizing galactooligosaccharides by cell-surface display of β-galactosidase on yarrowia alternative strategy for synthesizing galactooligosaccharides (gos) from an erythritol-producing yeast yarrowia lipolytica using surface display technology was demonstrated. the engineered strain cgmcc11369 was developed by fusion of the β-galactosidase gene from aspergillus oryzae to the ylpir1 gene, which codes for a cell wall protein. β-galactosidase was effectively displayed on the cell surface of yarrowia lipolytica start strain cgmcc7326. this engineered strain with surface-displayed β- ...201627090877
antiviral and cytotoxic isocoumarin derivatives from an endophytic fungus aspergillus oryzae.oryzaeins a-d (1-4), four new isocoumarin derivatives, along with five known ones (5-9) were isolated from solid cultures of an endophytic fungus aspergillus oryzae. their structures were elucidated by detailed spectroscopic analysis and by comparison with reported data of related derivatives. among them, compounds 1 and 2 represent the first examples of isocoumarins possessing an unusual 2-oxopropyl group and a rare 3-hydroxypropyl group. compounds 1 and 2 displayed moderate anti-tobacco mosaic ...201626824624
astellifadiene: structure determination by nmr spectroscopy and crystalline sponge method, and elucidation of its biosynthesis.genome mining of a terpene synthase gene from emericella variecolor nbrc 32302 and its functional expression in aspergillus oryzae led to the production of the new sesterterpene hydrocarbon, astellifadiene (1), having a 6-8-6-5-fused ring system. the structure of 1 was initially investigated by extensive nmr analyses, and was further confirmed by the crystalline sponge method, which established the absolute structure of 1 and demonstrated the usefulness of the method in the structure determinati ...201627038368
anti-obesity effect of extract from fermented curcuma longa l. through regulation of adipogenesis and lipolysis pathway in high-fat diet-induced obese rats.even though curcuma longa l. possesses various biological activities, it has strong flavor and taste, which decrease consumer palatability and limit industrial applications in food.201626822962
enhancement of fructosyltransferase and fructooligosaccharides production by a. oryzae dia-mf in solid-state fermentation using aguamiel as culture medium.the aim of this work was to improve the production of fructosyltransferase (ftase) by solid-state fermentation (ssf) using aguamiel (agave sap) as culture medium and aspergillus oryzae dia-mf as producer strain. ssf was carried out evaluating the following parameters: inoculum rate, incubation temperature, initial ph and packing density to determine the most significant factors through box-hunter and hunter design. the significant factors were then further optimized using a box-behnken design an ...201627036329
synthesis of oligosaccharides derived from lactulose (oslu) using soluble and immobilized aspergillus oryzae β-galactosidase.β-galactosidase from aspergillus oryzae offers a high yield for the synthesis of oligosaccharides derived from lactulose (oslu) by transgalactosylation. oligosaccharides with degree of polymerization (dp) ≥ 3 have shown to possess higher in vitro bifidogenic effect than di- and tetrasaccharides. thus, in this work, an optimization of reaction conditions affecting the specific selectivity of a. oryzae β-galactosidase for synthesis of oslu has been carried out to enhance oslu with dp ≥ 3 productio ...201627014684
development of enzyme technology for aspergillus oryzae, a. sojae, and a. luchuensis, the national microorganisms of japan.this paper describes the modern enzymology in japanese bioindustries. the invention of takadiastase by jokiti takamine in 1894 has revolutionized the world of industrial enzyme production by fermentation. in 1949, a new γ-amylase (glucan 1,4-α-glucosidase, ec from a. luchuensis (formerly designated as a. awamori), was found by kitahara. rnase t1 (guanyloribonuclease, ec was discovered by sato and egami. ando discovered aspergillus nuclease s1 (single-stranded nucleate endonucl ...201627151561
identification and manipulation of the pleuromutilin gene cluster from clitopilus passeckerianus for increased rapid antibiotic production.semi-synthetic derivatives of the tricyclic diterpene antibiotic pleuromutilin from the basidiomycete clitopilus passeckerianus are important in combatting bacterial infections in human and veterinary medicine. these compounds belong to the only new class of antibiotics for human applications, with novel mode of action and lack of cross-resistance, representing a class with great potential. basidiomycete fungi, being dikaryotic, are not generally amenable to strain improvement. we report identif ...201627143514
preventive effects of fermented brown rice and rice bran against prostate carcinogenesis in trap rats.fermented brown rice and rice bran with aspergillus oryzae (fbra) is considered to have the potential to prevent chemically-induced carcinogenesis in multiple organs of rodents. in the present study, we evaluated the possible chemopreventive effects of fbra against prostate tumorigenesis. six-week-old male rats of the transgenic rat for adenocarcinoma of prostate (trap) strain were fed diets containing 5% or 10% fbra for 15 weeks. animals were sacrificed at 21 weeks of age, and the ventral and l ...201627409632
chirally pure prodrugs and their converting enzymes lead to high supersaturation and rapid transcellular permeation of benzodiazepines.water-soluble prodrugs can be rapidly converted by enzymes to hydrophobic drugs, whose aqueous thermodynamic solubilities are low, but are maintained in aqueous solution at supersaturated concentrations due to slow precipitation kinetics. recently, we investigated avizafone (avf) in combination with aspergillus oryzae protease as a prodrug/enzyme system intended to produce supersaturated diazepam (dzp). several fold enhancement of permeation of supersaturated dzp across madin-darby canine kidney ...201627342435
molecular cloning and characterization of a novel bi-functional α-amylase/subtilisin inhibitor from hevea brasiliensis.a novel cdna encoding a bi-functional α-amylase/subtilisin inhibitor (hbasi) was isolated from rubber (hevea brasiliensis) leaves cultivar rrim600. the hbasi had strong homology with the soybean trypsin inhibitor (kunitz) family of protease inhibitors. its putative amino acid sequence was similar to that of the α-amylase/subtilisin inhibitor from ricinus communis (72% identity). genomic sequencing indicated that the hbasi gene contained no introns. the messenger rna of hbasi was detected in leaf ...201626854410
efficient kinetic resolution of (rs)-1-phenylethanol by a mycelium-bound lipase from a wild-type aspergillus oryzae strain.a mycelium-bound lipase from aspergillus oryzae (aol) exhibited excellent enantioselectivity for kinetic resolution of (rs)-1-phenylethanol in organic solvent. the various reaction parameters affecting the conversion and enantioselectivity were studied, including type of acyl donor, solvent, molar ratio, temperature, enzyme amount and substrate concentration. the optimum reaction conditions were found to be transesterification with vinyl acetate at 30°c in methyl tert-butyl ether with a vinyl ac ...201626854002
a novel non-thermostable deuterolysin from aspergillus oryzae.three putative deuterolysin (ec genes (deua, deub, and deuc) were found in the aspergillus oryzae genome database ( ). one of these genes, deua, was corresponding to npii gene, previously reported. deua and deub were overexpressed by recombinant a. oryzae and were purified. the degradation profiles against protein substrates of both enzymes were similar, but deub showed wider substrate specificity against peptidyl mca-substrates compared ...201627050120
metabolomic profiles of aspergillus oryzae and bacillus amyloliquefaciens during rice koji fermentation.rice koji, used early in the manufacturing process for many fermented foods, produces diverse metabolites and enzymes during fermentation. using gas chromatography time-of-flight mass spectrometry (gc-tof-ms), ultrahigh-performance liquid chromatography linear trap quadrupole ion trap tandem mass spectrometry (uhplc-ltq-it-ms/ms), and multivariate analysis we generated the metabolite profiles of rice koji produced by fermentation with aspergillus oryzae (rk_ao) or bacillus amyloliquefaciens (rk_ ...201627314317
a novel organic solvent- and detergent-stable serine alkaline protease from trametes cingulata strain ctm10101.a protease-producing fungus was isolated from an alkaline wastewater of chemical industries and identified as trametes cingulata strain ctm10101 on the basis of the its rdna gene-sequencing. it was observed that the fungus strongly produce extracellular protease grown at 30°c in potato-dextrose-broth (pdb) optimized media (13500u/ml). the pure serine protease isolated by trametes cingulata (designated sptc) was purified by ammonium sulfate precipitation-dialysis followed by heat-treatment and un ...201627296442
using a flexible shaft agitator to enhance the rheology of a complex fungal fermentation culture.the rheology behavior of biological fluids particularly when the viscosity is high and rheology is complex, is an important issue to understand, particularly for studies in mass-transfer and for solving technical problems with mixing in stirred bioreactors. in this paper, the use of a swingstir(®) impeller during the fermentation of aspergillus oryzae resulted in decreases from the parameters of a power-law model, in viscosity and in the thixotropic behavior of a cultivation broth. the results s ...201627438373
effect of mechanical denaturation on surface free energy of protein powders.globular proteins are important both as therapeutic agents and excipients. however, their fragile native conformations can be denatured during pharmaceutical processing, which leads to modification of the surface energy of their powders and hence their performance. lyophilized powders of hen egg-white lysozyme and β-galactosidase from aspergillus oryzae were used as models to study the effects of mechanical denaturation on the surface energies of basic and acidic protein powders, respectively. t ...201627434157
structural elucidation of enzymatically synthesized galacto-oligosaccharides using ion-mobility spectrometry-tandem mass spectrometry.galacto-oligosaccharides (gos) represent a diverse group of well-characterized prebiotic ingredients derived from lactose in a reaction catalyzed with β-galactosidases. enzymatic transgalactosylation results in a mixture of compounds of various degrees of polymerization and types of linkages. because structure plays an important role in terms of prebiotic activity, it is of crucial importance to provide an insight into the mechanism of transgalactosylation reaction and occurrence of different ty ...201627109424
molecular dissection of so (soft) protein in stress-induced aggregation and cell-to-cell interactive functions in filamentous fungal multicellularity.filamentous fungi grow by organizing multicellularity through hyphal compartmentalization and cell fusion. so (soft) protein, which was originally identified in neurospora crassa, plays distinct functional roles in cell-to-cell interactions, such as septal plugging and cell fusion. we previously reported that aoso, an aspergillus oryzae so homologue, forms aggregates at the septal pore in response to stress, as well as upon hyphal wounding. however, the functional regions that mediate the multic ...201627109373
aspergillus oryzae nrta affects kojic acid production.we analyzed the role of the nitrate transporter-encoding gene (nrta) of aspergillus oryzae by gene disruption. southern hybridization analysis indicated that homologous recombination occurred at the resident nrta locus. real-time pcr showed that the nrta gene was strongly inducible by nano3. the nrta disruptant did not exhibit normal growth when nitrate was available as the sole nitrogen source. these results indicate that nrta is essential for nitrate uptake in a. oryzae. kojic acid (ka) produc ...201627108780
comparative genomic analysis identified a mutation related to enhanced heterologous protein production in the filamentous fungus aspergillus oryzae.genomic mapping of mutations using next-generation sequencing technologies has facilitated the identification of genes contributing to fundamental biological processes, including human diseases. however, few studies have used this approach to identify mutations contributing to heterologous protein production in industrial strains of filamentous fungi, such as aspergillus oryzae. in a screening of a. oryzae strains that hyper-produce human lysozyme (hly), we previously isolated an aut1 mutant tha ...201627405439
identification and heterologous expression of the topopyrone nonaketide synthase gene from phoma sp.non-reducing iterative type i polyketide synthase genes, pnk1 and pnk2, were cloned from the fungus phoma sp. baua2861, which produces the topoisomerase i inhibitors, topopyrones a to d. heterologous expression of these polyketide synthase genes under the α-amylase promoter in aspergillus oryzae was carried out to identify their functions. the pnk2 transformant produced topopyrones c, d, and haematommone. therefore, the pnk2 gene was found to encode for the topopyrone nonaketide synthase.201627373651
structural characterization, catalytic, kinetic and thermodynamic properties of aspergillus oryzae tannase.tannase (ec. from aspergillus oryzae was purified using ammonium sulphate precipitation (75%), gel filtration chromatography through sephadex g-100, and g-200. the purified enzyme was monomeric protein with a molecular mass of 106kda. the activation energy for tannic acid hydrolysis was 32.6kjmol(-1) and its temperature quotient (q10) was 1.0. the pka1 and pka2 values of acidic and basic limbs of the active site residues were 4.6 and 6.4. the calculated values of thermodynamic parameter ...201627373426
potential pitfalls associated with testing of enzyme preparations in the salmonella/microsome assay.the effect of a sample of food enzyme preparations on s9 activity was evaluated in bacterial mutation assays with the salmonella typhimurium strains ta98 and ta100 using benzo(a)pyrene, 2-aminoanthracene and 2-aminofluorene as model compounds. under the experimental conditions applied, aspergillus oryzae protease and porcine pancreas trypsin, applied at low non-toxic doses, proved to effectively inhibit the metabolic activation of benzo(a)pyrene by aroclor induced rat liver 9, while the activati ...201627330008
six independent fucose-binding sites in the crystal structure of aspergillus oryzae lectin.the crystal structure of aol (a fucose-specific lectin of aspergillus oryzae) has been solved by sad (single-wavelength anomalous diffraction) and mad (multi-wavelength anomalous diffraction) phasing of seleno-fucosides. the overall structure is a six-bladed β-propeller similar to that of other fucose-specific lectins. the fucose moieties of the seleno-fucosides are located in six fucose-binding sites. although the arg and glu/gln residues bound to the fucose moiety are common to all fucose-bind ...201627318092
regioselective dichlorination of a non-activated aliphatic carbon atom and phenolic bismethylation by a multifunctional fungal flavoenzyme.the regioselective functionalization of non-activated carbon atoms such as aliphatic halogenation is a major synthetic challenge. a novel multifunctional enzyme catalyzing the geminal dichlorination of a methyl group was discovered in aspergillus oryzae (koji mold), an important fungus that is widely used for asian food fermentation. a biosynthetic pathway encoded on two different chromosomes yields mono- and dichlorinated polyketides (diaporthin derivatives), including the cytotoxic dichlorodia ...201627559694
artificial modification of an enzyme for construction of cross-reactive polyion complexes to fingerprint signatures of proteins and mammalian cells.a novel strategy for construction of cross-reactive enzyme-based sensors have been developed based on chemical modification of lysine ε-nh3(+) groups in β-galactosidase (β-gal) from aspergillus oryzae with various acid anhydrides. modification of lysine side chains markedly altered the kinetic parameters of β-gal (km and kcat), whereas catalytic activity remained and the tertiary structure was maintained for all modified β-gals. the addition of cationic pegylated polymers to reactive solutions c ...201627548408
evaluation of commercial soy sauce koji strains of aspergillus oryzae for γ-aminobutyric acid (gaba) this study, four selected commercial strains of aspergillus oryzae were collected from soy sauce koji. these a. oryzae strains designated as nsk, nsz, nsj and nst shared similar morphological characteristics with the reference strain (a. oryzae frr 1675) which confirmed them as a. oryzae species. they were further evaluated for their ability to produce γ-aminobutyric acid (gaba) by cultivating the spore suspension in a broth medium containing 0.4 % (w/v) of glutamic acid as a substrate for ga ...201627541157
overproduction, purification and characterization of adenylate deaminase from aspergillus oryzae.adenylate deaminase (ampd, ec is an aminohydrolase that widely used in the food and medicine industries. in this study, the gene encoding aspergillus oryzae ampd was cloned and expressed in escherichia coli. induction with 0.75 mm isopropyl β-d-l-thiogalactopyranoside resulted in an enzyme activity of 1773.9 u/ml. recombinant ampd was purified to electrophoretic homogeneity using nickel affinity chromatography, and its molecular weight was calculated as 78.6 kda. purified ampd exhibited ...201627539573
expression, purification and identification of a thermolysin-like protease, neutral protease i, from aspergillus oryzae with the pichia pastoris expression system.neutral proteases are widely used in the textile, food and medical industries. this study was designed to obtain high expression levels of neutral protease i from aspergillus oryzae 3.042 by using pichia pastoris gs115 as the host strain for industrial purposes. the coding sequence of the target gene was modified, synthesized, and then cloned into the expression vector phbm905bdm, which harbored the d1+2 × 201 aox1 promoter and the mf4i leader sequence. the recombinant plasmid was transformed in ...201627539551
japanese traditional dietary fungus koji aspergillus oryzae functions as a prebiotic for blautia coccoides through glycosylceramide: japanese dietary fungus koji is a new prebiotic.the japanese traditional cuisine, washoku, considered to be responsible for increased longevity among the japanese, comprises various foods fermented with the non-pathogenic fungus aspergillus oryzae (koji). we have recently revealed that koji contains an abundant amount of glycosylceramide. intestinal microbes have significant effect on health. however, the effects of koji glycosylceramide on intestinal microbes have not been studied.201627563516
screening for antimicrobial activity of wood rotting higher basidiomycetes mushrooms from uruguay against this work, the antimicrobial activity of extracts of wood rotting higher basidiomycetes mushrooms isolated from eucalyptus plantations in uruguay was studied using bacterial and fungal phytopathogens as targets. fifty-one extracts from mycelia and growth broth were prepared from higher basidiomycetes mushrooms, from which eight extracts (from ganoderma resinaceum, laetiporus sulphureus, dictyopanus pusillus, and bjerkandera adusta) showed antimicrobial activity against xanthomonas vesicatoria ...201627481160
comparison of the effects of dietary single and multi-probiotics on growth, non-specific immune responses and disease resistance in starry flounder, platichthys 8-week feeding trial was conducted to evaluate the effects of dietary probiotics on growth performance and non-specific immune responses in starry flounder, platichthys stellatus. fish averaging 46.5 ± 0.65 g (mean ± sd) were fed one of the six experimental diets; one control (cont), and five other diets were prepared by supplementing single-probiotics 1 (bacillus subtilis; sp1, 2 × 10(9) cfu kg(-1) diet), single-probiotics 2 (bacillus licheniformis; sp2, 2 × 10(9) cfu kg(-1) diet), multi-pro ...201627818339
aflatoxin b1 detoxification by aspergillus oryzae from meju, a traditional korean fermented soybean starter.aflatoxins are classified as group 1 (carcinogenic to humans) by the international agency for research on cancer (iarc). in this study, a total of 134 fungal strains were isolated from 65 meju samples, and two fungal isolates were selected as potential aflatoxin b₁ (afb₁)-biodetoxification fungi. these fungi were identified as aspergillus oryzae mao103 and a. oryzae mao104 by sequencing the beta-tubulin gene. the two a. oryzae strains were able to degrade more than 90% of afb1 (initial concentra ...201627817189
functional characterization of cyp52g3 from aspergillus oryzae and its application for bioconversion and synthesis of hydroxyl flavanone and steroids.aspergillus oryzae is a fungus widely used in traditional japanese fermentation industries. cytochrome p450 (cyp) proteins are ubiquitously distributed in nature and display a broad range of enzymatic activities. a novel cyp52 (cyp52g3) gene was found in a. oryzae. in this study, we report the functional characterization of cyp52g3. the recombinant protein was expressed heterologously in escherichia coli, and its membrane fraction isolated. cyp52g3 showed activities for 7-ethoxycoumarin and α-na ...201627028566
valorization of by-products from palm oil mills for the production of generic fermentation media for microbial oil synthesis.this study demonstrates the production of a generic nutrient-rich feedstock using by-product streams from palm oil production that could be used as a substitute for commercial fermentation supplements. solid-state fermentations of palm kernel cake (pkc) and palm-pressed fiber (ppf) were conducted in tray bioreactors and a rotating drum bioreactor by the fungal strain aspergillus oryzae for the production of crude enzymes. the production of protease was optimized (319.3 u/g) at an initial moistur ...201627787766
a glycoprotein α-amylase inhibitor from withania somnifera differentially inhibits various α-amylases and affects the growth and development of tribolium castaneum.identification and characterisation of plant defensive molecules enrich our resources to design crop protection strategies. in particular, plant-derived proteinaceous inhibitor(s) of insect digestive enzymes appear to be a safe, sustainable and attractive option.201627770482
mitochondria-localized phospholipase a2, aoplaa, in aspergillus oryzae displays phosphatidylethanolamine-specific activity and is involved in the maintenance of mitochondrial phospholipid mammals, cytosolic phospholipases a2 (cpla2s) play important physiological roles by releasing arachidonic acid, a precursor for bioactive lipid mediators, from the biological membranes. in contrast, fungal cpla2-like proteins are much less characterized and their roles have remained elusive. aoplaa is a cpla2-like protein in the filamentous fungus aspergillus oryzae which, unlike mammalian cpla2, localizes to mitochondria. in this study, we investigated the biochemical and physiological funct ...201627634187
subcellular localization of acyl-coa binding protein in aspergillus oryzae is regulated by autophagy eukaryotic cells, acyl-coa binding protein (acbp) is important for cellular activities, such as in lipid metabolism. in the industrially important fungus aspergillus oryzae, the acbp, known as aoacbp, has been biochemically characterized, but its physiological function is not known. in the present study, although we could not find any phenotype of aoacbp disruptants in the normal growth conditions, we examined the subcellular localization of aoacbp to understand its physiological function. us ...201627725156
fungal fermentation of lignocellulosic biomass for itaconic and fumaric acid production.the production of high-value chemicals from natural resources as alternative of petroleum-based products is currently expanding in parallel with biorefinery. the use of lignocellulosic biomass as raw material is promising to achieve economic and environmental sustainability. filamentous fungi, particularly aspergillus species, are already used industrially to produce organic acid as well as many enzymes. the production of lignocellulose degrading enzymes opens the possibility for direct fungal f ...201627666988
effect of different amylases on the utilization of cornstarch in broiler chickens.this study compared the effect of different amylases on the utilization of cornstarch in broiler chickens fed a corn-based diet. three-day-old arbor acres plus male chickens were randomly divided into 7 treatments and fed a diet supplemented with different sources and concentrations of amylase: 1,500 u/kg and 3,000 u/kg α-1,4 amylase from aspergillus oryzae (α-amylase a); 480 u/kg and 960 u/kg α-1,4 amylase from bacillus subtilis (α-amylase b); 200 u/kg and 400 u/kg α-1,6 isoamylase from b. subt ...201627664199
biosynthesis of shearinine: diversification of a tandem prenyl moiety of fungal indole diterpenes.the late-stage biosynthetic pathway of the indole diterpene shearinine involving four enzymatic reactions (janqdoj) was elucidated by an efficient heterologous expression system using aspergillus oryzae. key oxidative cyclization, forming a characteristic a/b bicyclic shearinine core by flavoprotein oxidase, was studied using a substrate analogue and a buffer containing h2(18)o. these experimental data provided evidence that jano catalyzes two-step oxidation via a hydroxylated product and that t ...201627632559
identification of a novel sesquiterpene biosynthetic machinery involved in astellolide biosynthesis.esterified drimane-type sesquiterpene lactones such as astellolides display various biological activities and are widely produced by plants and fungi. given their low homology to known sesquiterpene cyclases, the genes responsible for their biosynthesis have not been uncovered yet. here, we identified the astellolide gene cluster from aspergillus oryzae and discovered a novel sesquiterpene biosynthetic machinery consisting of astc, asti, and astk. all these enzymes are annotated as haloacid deha ...201627628599
optimized expression of prolyl aminopeptidase in pichia pastoris and its characteristics after glycosylation.prolyl aminopeptidases are specific exopeptidases that catalyze the hydrolysis of the n-terminus proline residue of peptides and proteins. in the present study, the prolyl aminopeptidase gene (pap) from aspergillus oryzae jn-412 was optimized through the codon usage of pichia pastoris. both the native and optimized pap genes were inserted into the expression vector ppic9 k and were successfully expressed in p. pastoris. additionally, the activity of the intracellular enzyme expressed by the reco ...201627628336
erratum to: japanese traditional dietary fungus koji aspergillus oryzae functions as a prebiotic for blautia coccoides through glycosylceramide: japanese dietary fungus koji is a new prebiotic.[this corrects the article doi: 10.1186/s40064-016-2950-6.].201627822443
functional and transcriptomic analysis of the key unfolded protein response transcription factor haca in aspergillus oryzae.haca is a conserved basic leucine zipper transcription factor that serves as the master transcriptional regulator in the unfolded protein response (upr). to comprehensively evaluate the role of haca in aspergillus oryzae, a homokaryotic haca disruption mutant (haca-de) and a strain that expressed a constitutively active form of haca (haca-ca) were successfully generated, and transcriptome analyses of these mutants were performed. growth and phenotypic profiles demonstrated that hyphal growth and ...201627520586
secretory expression, purification, characterization, and application of an aspergillus oryzae prolyl aminopeptidase in bacillus subtilis.cdna coding a prolyl aminopeptidase (pap) was cloned from aspergillus oryzae and over expressed in bacillus subtilis with a 6×his tag in n-terminus. the recombinant prolyl aminopeptidase was secreted to extracellular by adding 2 mm cacl2 and 5% d-sorbitol in tb medium; the enzyme activity in fermented supernatant increased from 7.2 to 41.5 u ml(-1). it has been purified 4.3-fold through ni-chelating affinity chromatography with a recovery of 47.3%. the purified enzyme is stable below 50 °c and w ...201627889870
rice koji extract enhances lipid metabolism through proliferator-activated receptor alpha (pparα) activation in mouse liver.koji is made from grains fermented with aspergillus oryzae and is essential for the production of many traditional japanese foods. many previous studies have shown that koji contributes to the improvement of dyslipidemia. however, little is known regarding the underlying mechanism of this effect. furthermore, the compound contributing to the activation of lipid metabolism is unknown. we demonstrated that rice koji extract (rke) induces the mrna expression of peroxisome proliferator-activated rec ...201627934292
evaluation of pyrolysis oil as carbon source for fungal fermentation.pyrolysis oil, a complex mixture of several organic compounds, produced during flash pyrolysis of organic lignocellulosic material was evaluated for its suitability as alternative carbon source for fungal growth and fermentation processes. therefore several fungi from all phyla were screened for their tolerance toward pyrolysis oil. additionally aspergillus oryzae and rhizopus delemar, both established organic acid producers, were chosen as model organisms to investigate the suitability of pyrol ...201628066378
simultaneous hydrolysis and co-fermentation of whey lactose with wheat for ethanol production.whey permeate was used as a co-substrate to replace part of the wheat for ethanol production by saccharomyces cerevisiae. the simultaneous saccharification and fermentation was achieved with β-galactosidase added at the onset of the fermentation to promote whey lactose hydrolysis. aspergillus oryzae and kluyveromyces lactis β-galactosidases were two enzymes selected and used in the co-fermentation of wheat and whey permeate for the comparison of their effectiveness on lactose hydrolysis. the pos ...201627693727
novel inexpensive fungi proteases: production by solid state fermentation and characterization.a comparative study was carried out for proteases production using agroindustrial residues as substrate for solid state fermentation (ssf) of several fungal strains. high protease production was observed for most of the microorganisms studied, as well as very different biochemical characteristics, including activities at specific temperatures and a wide range of ph values. the enzymes produced were very different regarding optimum ph and they showed stability at 50 °c. aspergillus oryzae showed ...201626769513
genome mining and motif truncation of glycoside hydrolase family 5 endo-β-1,4-mannanase encoded by aspergillus oryzae rib40 for potential konjac flour hydrolysis or feed additive.two novel glycosyl hydrolase family 5 (gh5) β-mannanases (aoman5a and aoman5b) were identified from aspergillus oryzae rib40 by genome mining. the aoman5a contains a predicted family 1 carbohydrate binding module (cbm-1), located at its n-terminal. the aoman5a, aoman5b and truncated mutant aoman5aδcl (truncating the n-terminal cbm and linker of aoman5a) were expressed retaining the n-terminus of the native protein in pichia pastoris gs115 by ppic9k(m). the specific enzyme activity of the purifie ...201627702490
aspergillus oryzae-saccharomyces cerevisiae consortium allows bio-hybrid fuel cell to run on complex carbohydrates.consortia of aspergillus oryzae and saccharomyces cerevisiae are examined for their abilities to turn complex carbohydrates into ethanol. to understand the interactions between microorganisms in consortia, fourier-transform infrared spectroscopy is used to follow the concentrations of various metabolites such as sugars (e.g., glucose, maltose), longer chain carbohydrates, and ethanol to optimize consortia conditions for the production of ethanol. it is shown that with proper design a. oryzae can ...201627681904
valorization of sugar-to-ethanol process waste vinasse: a novel biorefinery approach using edible ascomycetes filamentous fungi.the aim of the present work was to study the integration of edible ascomycetes filamentous fungi into the existing sugar- or molasses-to-ethanol processes, to grow on vinasse or stillage and produce ethanol and protein-rich fungal biomass. two fungal strains, neurospora intermedia and aspergillus oryzae were examined in shake flasks and airlift-bioreactors, resulting in reduction of vinasse cod by 34% and viscosity by 21%. utilization of glycerol and sugars were observed, yielding 202.4 or 222.8 ...201627668880
degradation of 3-phenoxybenzoic acid by a filamentous fungus aspergillus oryzae m-4 strain with self-protection transformation.a novel filamentous fungus m-4 strain was isolated from soy sauce koji and identified as aspergillus oryzae (collection number: cgmcc 11645) on the basis of morphological characteristics and internal transcribed spacer sequence. m-4 could degrade 80.62 % of 3-phenoxybenzoic acid (3-pba; 100 mg l(-1)) within 5 days. 3-pba degradation occurred in accordance with first-order kinetics. the degradation metabolites of 3-pba were identified through high-performance liquid chromatography-mass spectromet ...201627678114
nutrient assessment of olive leaf residues processed by solid-state fermentation as an innovative feedstuff additive.olive leaf residue feedstuff additives were prepared by solid-state fermentation (ssf), and its feeding effects on broiler chickens were examined.201626991541
a set of isogenic auxotrophic strains for constructing multiple gene deletion mutants and parasexual crossings in aspergillus construct a set of isogenic auxotrophic strains in aspergillus niger suited for creating multiple gene deletion mutants and executing parasexual crossings, we have combined mutations in genes involved in colour pigmentation (fwna and olva) with well-selectable auxotrophic markers (pyrg, nicb, argb, and adea). all markers, except for the pyrg marker, were introduced by targeted deletion, omitting uv mutagenesis of the strains. aspergillus oryzae orthologous genes of the argb, nicb, and adea ma ...201627251039
in vitro cytotoxicity and antimicrobial activity of talaromyces flavus sp5 inhabited in the marine sediment of southern coast of india.marine sediment samples were collected from the coastal areas of southern india, particularly in kanyakumari district. twenty-eight different fungal strains were isolated. the screening of fungi from marine sediment was done to isolate a potent fungus that can produce bioactive compounds for biomedical applications. only three strains viz trichoderma gamsii sp4, talaromyces flavus sp5 and aspergillus oryzae sp6 were screened for further studies. the intracellular bioactive compounds were extract ...201628262118
genome sequence of aspergillus luchuensis nbrc 4314.awamori is a traditional distilled beverage made from steamed thai-indica rice in okinawa, japan. for brewing the liquor, two microbes, local kuro (black) koji mold aspergillus luchuensis and awamori yeast saccharomyces cerevisiae are involved. in contrast, that yeasts are used for ethanol fermentation throughout the world, a characteristic of japanese fermentation industries is the use of aspergillus molds as a source of enzymes for the maceration and saccharification of raw materials. here we ...201627651094
application of lectin array technology for biobetter characterization: its correlation with fcγriii binding and adcc.lectin microarray technology was applied to compare the glycosylation pattern of the monoclonal antibody mb311 expressed in sp2.0 cells to an antibody-dependent cellular cytotoxic effector function (adcc)-optimized variant (mb314). mb314 was generated by a plant expression system that uses genetically modified moss protoplasts (physcomitrella patens) to generate a de-fucosylated version of mb311. in contrast to mb311, no or very low interactions of mb314 with lectins aspergillus oryzae l-fucose ...201628029136
biohydrogen production in the suspended and attached microbial growth systems from waste pastry hydrolysate.waste pastry was hydrolyzed by glucoamylase and protease which were obtained from solid state fermentation of aspergillus awamori and aspergillus oryzae to produce waste pastry hydrolysate. then, the effects of hydraulic retention times (hrts) (4-12h) on hydrogen production rate (hpr) in the suspended microbial growth system (continuous stirred tank reactor, cstr) and attached microbial growth system (continuous mixed immobilized sludge reactor, cmisr) from waste pastry hydrolysate were investig ...201627416509
transformation of litchi pericarp-derived condensed tannin with aspergillus awamori.condensed tannin is a ubiquitous polyphenol in plants that possesses substantial antioxidant capacity. in this study, we have investigated the polyphenol extraction recovery and 2,2-diphenyl-1-picrylhydrazyl (dpph) scavenging activity of the extracted polyphenol after litchi pericarp is treated with aspergillus awamori, aspergillus sojae or aspergillus oryzae. we have further explored the activity of a. awamori in the formation of condensed tannin. the treatment of a. awamori appeared to produce ...201627420043
an overproduction of astellolides induced by genetic disruption of chromatin-remodeling factors in aspergillus oryzae.the filamentous fungus aspergillus oryzae is an important industrial mold. recent genomic analysis indicated that a. oryzae has a large number of biosynthetic genes for secondary metabolites (sms), but many of the sms they produce have not been identified. for better understanding of sms production by a. oryzae, we screened a gene-disruption library of transcription factors including chromatin-remodeling factors and found two gene disruptions that show similarly altered sm production profiles. o ...201626126743
structural and catalytic properties of s1 nuclease from aspergillus oryzae responsible for substrate recognition, cleavage, non-specificity, and inhibition.the single-strand-specific s1 nuclease from aspergillus oryzae is an archetypal enzyme of the s1-p1 family of nucleases with a widespread use for biochemical analyses of nucleic acids. we present the first x-ray structure of this nuclease along with a thorough analysis of the reaction and inhibition mechanisms and of its properties responsible for identification and binding of ligands. seven structures of s1 nuclease, six of which are complexes with products and inhibitors, and characterization ...201628036383
valorization of bakery waste for biocolorant and enzyme production by monascus purpureus.a concept of utilizing bakery waste as a nutrient source for the fermentative production of bio-colorant by monasucus purpureus has been developed. the proposed ideas provide an innovative approach to establish a system or method to reduce the bakery waste problem encountered by hong kong or other countries. bakery waste collected from bakery store was used in submerged and solid-state fermentation of monascus purpureus to produce bio-colorant, which could potentially be applied in food and text ...201627208753
continuous biohydrogen production from waste bread by anaerobic this study, continuous biohydrogen production from waste bread by anaerobic sludge was performed. the waste bread was first hydrolyzed by the crude enzymes which were generated by aspergillus awamori and aspergillus oryzae via solid-state fermentation. it was observed that 49.78g/l glucose and 284.12mg/l free amino nitrogen could be produced with waste bread mass ratio of 15% (w/v). the waste bread hydrolysate was then used for biohydrogen production by anaerobic sludge in a continuous stirre ...201627065225
increased enzyme production under liquid culture conditions in the industrial fungus aspergillus oryzae by disruption of the genes encoding cell wall α-1,3-glucan synthase.under liquid culture conditions, the hyphae of filamentous fungi aggregate to form pellets, which reduces cell density and fermentation productivity. previously, we found that loss of α-1,3-glucan in the cell wall of the fungus aspergillus nidulans increased hyphal dispersion. therefore, here we constructed a mutant of the industrial fungus a. oryzae in which the three genes encoding α-1,3-glucan synthase were disrupted (tripleδ). although the hyphae of the tripleδ mutant were not fully disperse ...201627442340
involvement of an srf-mads protein mcma in regulation of extracellular enzyme production and asexual/sexual development in aspergillus nidulans.srf-mads proteins are transcription factors conserved among eukaryotes that regulate a variety of cellular functions; however, their physiological roles are still not well understood in filamentous fungi. effects of a mutation in mcma gene that encodes the sole srf-mads protein in the fungus aspergillus nidulans were examined by rna sequencing. sequencing data revealed that expression levels of cellulase genes were significantly decreased by the mutation as reported previously. however, expressi ...201626967516
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