TitleAbstractYear(sorted descending)
identification of three mutant loci conferring carboxin-resistance and development of a novel transformation system in aspergillus oryzae.mutants exhibiting resistance to the fungicide, carboxin, were isolated from aspergillus oryzae, and the mutations in the three gene loci, which encode succinate dehydrogenase (sdh) b, c, and d subunits, were identified to be independently responsible for the resistance. a structural model of the sdh revealed the different mechanisms that confer carboxin-resistance in different mutations. the mutant aosdhb gene (aosdhb(cxr)) was further examined for possible use as a transformant selection marke ...200918992352
the effect of domestication on the fungal proteome.the molecular effect of domestication has rarely been examined in fungi. i examined the molecular signature of domestication on aspergillus oryzae, a fungus used to ferment several japanese food products. the ratio of nonsynonymous to synonymous substitutions and the percentage of estimated deleterious substitutions along the a. oryzae lineage were lower than those in the wild sister lineage aspergillus flavus. the patterns of genetic change observed in human-mediated domestication of animals an ...200919081651
biosensor based on laccase and an ionic liquid for determination of rosmarinic acid in plant extracts.novel biosensors based on laccase from aspergillus oryzae and the ionic liquids (ils) 1-n-butyl-3-methylimidazolium hexafluorophosphate (bmipf(6)) and 1-n-butyl-3-methylimidazolium tetrafluoroborate (bmibf(4)) were constructed for determination of rosmarinic acid by square-wave voltammetry. the laccase catalyzes the oxidation of rosmarinic acid to the corresponding o-quinone, which is electrochemically reduced back to rosmarinic acid at +0.2v vs. ag/agcl. the biosensor based on bmipf(6) showed a ...200919084643
novel easily accessible glucosidase inhibitors: 4-hydroxy-5-alkoxy-1,2-cyclohexanedicarboxylic acids.glycosidases are very important enzymes involved in a variety of biochemical processes with a special importance to biotechnology, food industry, and pharmacology. novel structurally simple inhibitors derived from cyclohexane-1,2-dicarboxylic acids were synthesized and tested against several fungal glycosidases from aspergillus oryzae and penicilliumcanescens. the presence of at least two carboxylic groups and one hydroxy group was essential for efficient inhibition. significant selective inhibi ...200919084826
mathematical modeling of the development of antioxidant activity in soybeans fermented with aspergillus oryzae and aspergillus awamori in the solid state.the kinetics of the development fungal growth, beta-glucosidase activity, total phenolics, and dpph scavenging in soybeans fermented with aspergillus oryzae and aspergillus awamori were studied over an 8-day incubation period. modified logistic equations were then used to describe the experimental fermentation profiles. in all cases the models were consistent and the parametric estimations were statistically significant. the predicted values have high coefficients of linear correlation with the ...200919099459
cloning and heterologous transcription of a polyketide synthase gene from the lichen solorina crocea.lichens and most ascomycete fungi produce polyketide secondary metabolites often with valuable biological activities. their biosynthesis is primarily governed by large iterative multifunctional type i polyketide synthases. although there has been good progress studying filamentous non-lichenized fungi, there is limited information on polyketide biosynthesis in lichens and their mycobionts, due to their slow growth, difficulties in establishing pure cultures, and the absence of methods for direct ...200919100326
construction of quintuple protease gene disruptant for heterologous protein production in aspergillus oryzae.aspergillus oryzae has received attention as a host for heterologous protein production. however, a. oryzae has 134 protease genes, which is recognized to be one of the major reasons for the proteolytic degradation of heterologously produced proteins. we previously reported that double disruption of the protease genes (tppa and pepe) improved heterologous protein (human lysozyme) production by a. oryzae. in this study, we performed successive round of five protease genes (tppa, pepe, nptb, dppiv ...200919107471
comparative analysis of oligosaccharide specificities of fucose-specific lectins from aspergillus oryzae and aleuria aurantia using frontal affinity chromatography.aleuria aurantia lectin (aal) is widely used to estimate the extent of alpha1,6-fucosylated oligosaccharides and to fractionate glycoproteins for the detection of specific biomarkers for developmental antigens. our previous studies have shown that aspergillus oryzae lectin (aol) reflects the extent of alpha1,6-fucosylation more clearly than aal. however, the subtle specificities of these lectins to fucose linked to oligosaccharides through the 2-, 3-, 4-, or 6-position remain unclear, because la ...200919109923
use of saccharum spontaneum (wild sugarcane) as biomaterial for cell immobilization and modulated ethanol production by thermotolerant saccharomyces cerevisiae vs3.saccharum spontaneum is a wasteland weed consists of 45.10+/-0.35% cellulose and 22.75+/-0.28% of hemicellulose on dry solid (ds) basis. aqueous ammonia delignified s. spontaneum yielded total reducing sugars, 53.91+/-0.44 g/l (539.10+/-0.55 mg/g of substrate) with a hydrolytic efficiency of 77.85+/-0.45%. the enzymes required for hydrolysis were prepared from culture supernatants of aspergillus oryzae mtcc 1846. a maximum of 0.85+/-0.07 iu/ml of filter paperase (fpase), 1.25+/-0.04 iu/ml of car ...200919114303
functional expression of miraculin, a taste-modifying protein in escherichia coli.miraculin isolated from red berries of richadella dulcifica, a native shrub of west africa, has the unusual property of modifying a sour taste into a sweet one. this homodimer protein consists of two glycosylated polypeptides that are cross-linked by a disulfide bond. recently, functional expression of miraculin was reported in host cells with the ability to glycosylate proteins, such as lettuce, tomato and the microbe aspergillus oryzae, but not escherichia coli. thus, a question remains as to ...200919122203
analysis of extracellular proteins of aspergillus oryzae grown on soy sauce koji.aspergillus oryzae as 3.951 is widely used in chinese soy sauce manufacture, but little is known about the profiles of the extracellular proteins from the culture of soybean koji. in this study, we carried out maldi-tof/tof ms analysis of extracellular proteins during koji culture. besides well-known proteins (taa and oryzin), a variety of aminopeptidase and proteases were identical at the proteome level. this suggests that a. oryzae as 3.951 has a powerful capacity to digest soybean protein.200919129648
alternative processing of proproteins in aspergilli kexb gene disruptants under hyperosmotic conditions.disruption of the kexb gene encoding a subtilisin-like processing protease in aspergillus oryzae and aspergillus nidulans led to remarkable morphological defects, and these phenotypes were suppressed under hyperosmotic conditions. in this study, we investigated to determine whether non-kexb proteases might complement the in vivo function of kexb in the two aspergillus kexb disruptants. neither overexpression of opsa or opsb encoding a. oryzae aspartyl proteases homologous to yeast yapsins (yps1/ ...200919129662
disruption of the aopex11-1 gene involved in peroxisome proliferation leads to impaired woronin body formation in aspergillus oryzae.the woronin body, a unique organelle found in the pezizomycotina, plugs the septal pore upon hyphal damage to prevent excessive cytoplasmic bleeding. although it was previously shown that the woronin body buds out from the peroxisome, the relationship between peroxisomal proliferation/division and woronin body differentiation has not been extensively investigated. in this report, we examined whether pex11 required for peroxisomal proliferation participates in woronin body formation in aspergillu ...200919136573
chemopreventive effects of fermented brown rice and rice bran against 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone-induced lung tumorigenesis in female a/j mice.the most common cause of human lung cancer is suggested to be exposure to the carcinogens in tobacco smoke. among the multiple chemicals in tobacco smoke, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (nnk) has been regarded as one of the important agents for generation of lung cancers. previously, our studies proved that fermented brown rice and rice bran (fbra) has chemopreventive effects against carcinogenesis of the colon, liver, stomach, bladder and esophagus. in the present study, we exam ...200919148502
isolation of aspergillus oryzae mutants for heterologous protein production from a double proteinase gene disruptant.aspergillus oryzae has attracted much attention as a host for heterologous protein production because of its high secretion ability and safety. however, there have been only a few reports on construction of this organism to improve its properties as a production host. we previously reported that the double disruptant of the proteinase gene (tppa, pepe) improved human lysozyme (hly) production. in this double disruptant, however, the hly expression plasmid cannot be removed due to its random inte ...200919151971
rapid screening and isolation of a fungus for sitosterol to androstenedione biotransformation.androstenedione (ad) is a steroid intermediate used in the pharmaceutical industry for the production of several important anabolic drugs. an important route for producing ad is by the microbial transformation of sterols. microbes capable of efficiently transforming sterols to ad are few and newer strains need to be isolated. conventional procedures for screening and isolation are time consuming. a new procedure was used for screening and isolation of fungal microorganisms capable of biotransfor ...200919189060
enzymatic hydrolysis of recovered protein from frozen small croaker and functional properties of its protein isolate were recovered from frozen small croaker using ph shift. the partial enzymatic hydrolysates were fractionated as soluble and insoluble parts. they were dried using the drum dryer and their functional properties were examined. the total nitrogen content of the enzymatic hydrolysates ranged from 12.9% to 13.7%. the degree of hydrolysis of precipitates was 18.2% and 12.2% for croaker hydrolysates treated with protamex 1.5 mg (bacilllus protease complex) and flavourzyme 500 mg ( ...200919200081
kinetic resolution of alpha-lipoic acid via enzymatic differentiation of a remote stereocenter.kinetic resolution of alpha-lipoic acid, a case of remote stereocenter discrimination, was accomplished using lipase from aspergillus oryzae wz007. performance of this lipase was investigated for enantioselective esterification of (s)-alpha-lipoic acid, leaving the target product (r)-alpha-lipoic acid in unreacted form. the effects of chain length of alcohol, type of solvent, molar ratio of alcohol:acid, and reaction temperature were studied. the optimum reaction conditions were found to be este ...200919212787
comparative analysis of core-fucose-binding lectins from lens culinaris and pisum sativum using frontal affinity chromatography.lens culinaris lectin (lca) is a useful probe for the detection in serum of a core-fucosylated alpha-fetoprotein, called afp-l3 fraction, which is a well-known marker for the diagnosis and prognosis of hepatocellular carcinoma. here we performed a systematic quantitative interaction analysis of lca and its close homolog, pisum sativum lectin (psa), by frontal affinity chromatography with 143 pyridylaminated (pa) glycans including a series of core-fucosylated glycans. both lectins showed binding ...200919218400
the dddp gene, encoding a novel enzyme that converts dimethylsulfoniopropionate into dimethyl sulfide, is widespread in ocean metagenomes and marine bacteria and also occurs in some ascomycete fungi.the marine alphaproteobacterium roseovarius nubinhibens ism can produce the gas dimethyl sulfide (dms) from dimethylsulfoniopropionate (dmsp), a widespread secondary metabolite that occurs in many phytoplankton. roseovarius possesses a novel gene, termed dddp, which when cloned, confers on escherichia coli the ability to produce dms. the dddp polypeptide is in the large family of m24 metallopeptidases and is wholly different from two other enzymes, dddd and dddl, which were previously shown to g ...200919220400
secretory expression of the non-secretory-type lentinula edodes laccase by aspergillus oryzae.the shiitake mushroom, lentinula edodes, has an extracelluar secretory-type laccase, lcc1, and a fruiting-body-accumulation-type laccase, lcc4. we previously reported the production of lcc1 by plant cells, but had difficulty producing lcc4. here, we report the production of lcc1 and lcc4 by aspergillus oryzae and the extracellular secretory production of lcc4 using a modified secretion signal peptide (sp) from lcc1. sp-lcc4 produced by a. oryzae had biochemical activities similar to lcc4 produce ...200919230633
enrichment of phenolics and free radical scavenging property of wheat koji prepared with two filamentous the present study, total phenolic content and antioxidant property of wheat (54% ethanolic extract) was drastically enhanced when fermented with two gras filamentous fungi, aspergillus oryzae and aspergillus awamori nakazawa. a. oryzae yielded significant (p<0.05) amounts of phenolic compounds, dpph* (2,2-diphenyl-1-picrylhydrazyl) and abts*(+) [2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid)] scavenging properties on 4th day of incubation compared to a. awamori on 5th day of incubatio ...200919232489
purification and characterization of intracellular cellulase from aspergillus oryzae itcc-4857.01.purification and characterization of intracellular cellulase produced by a. oryzae itcc-4857.01 are reported. the enzyme was purified by ion-exchange chromatography using deae-cellulose followed by gel filtration. the purification achieved was 41 fold from the crude extract with yield of 27%. the purified enzyme showed single band on poly acrylamide gel. the molecular weight as determined by sds-page and gel filtration was 38 kda and 38.6 kda respectively and contained only one subunit. the enzy ...200923983520
aspergillus flavus genomics as a tool for studying the mechanism of aflatoxin formation.aspergillus flavus is a weak pathogen that infects plants, animals and humans. when it infects agricultural crops, however, it produces one of the most potent carcinogens known (aflatoxins). to devise strategies to control aflatoxin contamination of pre-harvest agricultural crops and post-harvest grains during storage, we launched the a. flavus genomics program. the major objective of this program is the identification of genes involved in aflatoxin biosynthesis and regulation, as well as in pat ...200819238624
codon optimization prevents premature polyadenylation of heterologously-expressed cellulases from termite-gut symbionts in aspergillus achieve high expression of glycoside hydrolase family 45 endoglucanase (rssym45eg1) from a symbiotic protist of the termite reticulitermes speratus, synthetic sequence rssym45eg1-co, in which the codon usage was adjusted to that of the highly-expressed tef1 gene encoding translation elongation factor 1alpha, was prepared and introduced into a. oryzae. the transcript level of rssym45eg1-co was 1.8-fold higher than that of rssym45eg1. in cells harboring rssym45eg1, but not rssym45eg1-co, trunca ...200819164877
construction of a citrinin gene cluster expression system in heterologous aspergillus oryzae.filamentous fungi are considered an attractive resource for the discovery and production of bioactive compounds. to facilitate molecular breeding, biosynthetic genes must be rapidly identified. but, even after the chemical structure of a compound is identified, finding the corresponding biosynthetic genes in the fungal genome still remains a challenge. in an attempt to overcome this difficulty and to easily characterize each gene in a cluster, we constructed a heterologous expression system usin ...200819111642
isolation of industrial strains of aspergillus oryzae lacking ferrichrysin by disruption of the dffa gene.based on studies using laboratory strains, the efficiency of gene disruption in aspergillus oryzae, commonly known as koji mold, is low; thus, gene disruption has rarely been applied to the breeding of koji mold. to evaluate the efficiency of gene disruption in industrial strains of a. oryzae, we produced ferrichrysin biosynthesis gene (dffa) disruptants using three different industrial strains as hosts. pcr analysis of 438 pyrithiamine-resistant transformants showed dffa gene disruption efficie ...200819111645
a comparison of the unfolded protein response in solid-state with submerged cultures of aspergillus oryzae.the unfolded protein response (upr) is a regulatory system to maintain the homeostasis of er functions. here we report a comparison of express levels of upr relevant genes in aspergillus oryzae between solid-state and submerged cultivation. the results were that up-regulation of the upr mechanism in solid-state culture was higher than in submerged culture (heat-shock or non-stress conditions). this might have been a result of changing culture conditions.200818997417
generation of large chromosomal deletions in koji molds aspergillus oryzae and aspergillus sojae via a loop-out recombination.we established a technique for efficiently generating large chromosomal deletions in the koji molds aspergillus oryzae and a. sojae by using a ku70-deficient strain and a bidirectional marker. the approach allowed deletion of 200-kb and 100-kb sections of a. oryzae and a. sojae, respectively. the deleted regions contained putative aflatoxin biosynthetic gene clusters. the large genomic deletions generated by a loop-out deletion method (resolution-type recombination) enabled us to construct multi ...200818952883
development and evaluation of a real-time quantitative pcr assay for aspergillus flavus.aspergillus flavus is a ubiquitous mold and the most common mold contaminating foodstuffs. many strains of a. flavus produce aflatoxins. in addition it is an allergen and an opportunistic pathogen of animals and plants. a. flavus often is underestimated in traditional culture analyses due to the expertise required and the cost associated with speciating members of the genus aspergillus. the goal of this study was to develop and validate a primer and probe set for the rapid detection and quantita ...200818959152
identification of a novel polyketide synthase-nonribosomal peptide synthetase (pks-nrps) gene required for the biosynthesis of cyclopiazonic acid in aspergillus oryzae.cyclopiazonic acid (cpa) is a mycotoxin produced by several strains of penicillium and aspergillus species. aspergillus oryzae strains used in fermented foods do not produce cpa; however, several wild-type a. oryzae strains produce cpa. here, we identified a novel polyketide synthase-nonribosomal peptide synthetase (pks-nrps) gene involved in cpa production by comparing the telomere-adjacent region of a cpa-producing strain (a. oryzae nbrc 4177) with that of a nonproducing strain (a. oryzae rib4 ...200818854220
characterization of the aspergillus niger prtt, a unique regulator of extracellular protease encoding genes.expression of several aspergillus niger genes encoding major secreted, but not vacuolar, protease genes including the major acid protease gene pepa, was shown to be affected in the previously isolated a. niger protease mutant, ab1.13 [mattern, i.e., van noort, j.m., van den berg, p., archer, d.a., roberts, i.n., hondel, c.a.m.j.j., 1992. isolation and characterization of mutants of aspergillus niger deficient in extracellular proteases. molecular & general genetics 2, 332-336]. complementation c ...200818930158
characterization of an alpha-l-rhamnosidase from aspergillus kawachii and its alpha-l-rhamnosidase was purified by fractionating a culture filtrate of aspergillus kawachii grown on l-rhamnose as the sole carbon source. the alpha-l-rhamnosidase had a molecular mass of 90 kda and a high degree of n-glycosylation of approximately 22%. the enzyme exhibited optimal activity at ph 4.0 and temperature of 50 degrees c. further, it was observed to be thermostable, and it retained more than 80% of its original activity following incubation at 60 degrees c for 1 h. its t (50) val ...200818633609
direct ethanol production from barley beta-glucan by sake yeast displaying aspergillus oryzae beta-glucosidase and endoglucanase.three beta-glucosidase- and two endoglucanase-encoding genes were cloned from aspergillus oryzae, and their gene products were displayed on the cell surface of the sake yeast, saccharomyces cerevisiae gri-117-uk. gri-117-uk/pudb7 displaying beta-glucosidase ao090009000356 showed the highest activity against various substrates and efficiently produced ethanol from cellobiose. on the other hand, gri-117-uk/pudcb displaying endoglucanase ao090010000314 efficiently degraded barley beta-glucan to glu ...200818640601
stable repeated-batch production of recombinant dye-decolorizing peroxidase (rdyp) from aspergillus oryzae.recombinant aspergillus oryzae expressing a dye-decolorizing peroxidase gene (dyp) was cultivated for repeated-batch production of recombinant dye-decolorizing peroxidase (rdyp) using maltose as a carbon source. high-level rdyp activity in limitation of carbon and nitrogen sources was maintained stably for 26 cycles of repeated 1-d batches of a. oryzae pellets without any additional ph control. cultures maintained at 4 degrees c for 20 d resumed rdyp production following a single day of incubati ...200818640612
an open label study to determine the effects of an oral proteolytic enzyme system on whey protein concentrate metabolism in healthy males.current research suggests that protein intake of 1.5 - 2.8 g/kg/day (3.5 times the current recommended daily allowance) is effective and safe for individuals trying to increase or maintain lean muscle mass. to achieve these levels of daily protein consumption, supplementing the diet with processed whey protein concentrate (wpc) in liquid form has become a popular choice for many people. some products have a suggested serving size as high as 50 g of protein. however, due to possible inhibition of ...200818652668
identification and cloning of a gene encoding tannase (tannin acylhydrolase) from lactobacillus plantarum atcc 14917(t).the gene tanlpl, encoding a novel tannase enzyme (tanlpl), has been cloned from lactobacillus plantarum atcc 14917(t). this is the first report of a tannase gene cloned from a bacterial source other than from staphylococcus lugdunensis, which has been reported elsewhere. the open reading frame of tanlpl, spanning 1410 bp, encoded a 469-amino-acid protein that showed 28.8% identity to the tannase of s. lugdunensis with several commonly conserved sequences. these sequences could not be found in pu ...200818653299
improvement of aspergillus oryzae for hyperproduction of endoglucanase: expression cloning of cmc-1 gene of aspergillus (cmc1; family 12) is one of the endoglucanases of aspergillus aculeatus and consists of single polypeptide chain of 221 amino acids. the cmc1 gene was expressed in aspergillus oryzae niad300 (niad-) under promoter 8142. the plasmid pcmg14 carrying the cmc1 gene at psti site was used as a source of the gene (920 bp) and aspergillus oryzae was successfully transformed by the plasmid pnan-cmc1 (harboring cmc1 gene). the plasmid was integrated in aspergillus oryzae niad300 ...200818661108
effects of a casein hydrolysate prepared from aspergillus oryzae protease on adjuvant arthritis in rats.we evaluated the effects of a casein hydrolysate (ch) prepared from aspergillus oryzae protease on rat adjuvant arthritis, a model of human rheumatoid arthritis. ch was administered orally once a day to the animals for 22 d after the adjuvant injection. ch suppressed swelling in the adjuvant-uninjected hind paws, and a higher dose of ch suppressed the increase in arthritic score and swelling of the adjuvant-injected hind paws. a histopathological examination revealed evidence that the higher dos ...200818685219
therapeutic effects of a fermented soy product on peanut hypersensitivity is associated with modulation of t-helper type 1 and t-helper type 2 responses.immubalance is a koji fungus (aspergillus oryzae) and lactic acid fermented soybean product. this unique production process is believed to create a food supplement that helps to induce or maintain normal immune response.200818705693
elusive origins of the extra genes in aspergillus oryzae.the genome sequence of aspergillus oryzae revealed unexpectedly that this species has approximately 20% more genes than its congeneric species a. nidulans and a. fumigatus. where did these extra genes come from? here, we evaluate several possible causes of the elevated gene number. many gene families are expanded in a. oryzae relative to a. nidulans and a. fumigatus, but we find no evidence of ancient whole-genome duplication or other segmental duplications, either in a. oryzae or in the common ...200818725939
codon optimization increases steady-state mrna levels in aspergillus oryzae heterologous gene expression.we investigated the effect of codon optimization on the expression levels of heterologous proteins in aspergillus oryzae, using the mite allergen der f 7 as a model protein. a codon-optimized der f 7 gene was synthesized according to the frequency of codon usage in a. oryzae by recursive pcr. both native and optimized der f 7 genes were expressed under the control of a high-level-expression promoter with their own signal peptides or in a fusion construct with a. oryzae glucoamylase (glaa). codon ...200818791013
preparation and comparative characterization of immobilized aspergillus oryzae expressing fusarium heterosporum lipase for enzymatic biodiesel this paper, we provide the first report of utilizing recombinant fungal whole cells in enzymatic biodiesel production. aspergillus oryzae, transformed with a heterologous lipase-encoding gene from fusarium heterosporum, produced fully processed and active forms of recombinant f. heterosporum lipase (fhl). cell immobilization within porous biomass support particles enabled the convenient usage of fhl-producing a. oryzae as a whole-cell biocatalyst for lipase-catalyzed methanolysis. the additio ...200818795281
genomics of industrial aspergilli and comparison with toxigenic relatives.aspergillus oryzae has been used in japanese fermentation industries for more than a thousand years. the species produces large amounts of various hydrolytic enzymes and has been successfully applied to modern biotechnology. the size of the a. oryzae genome (37.5 mb) is very close to that of a. flavus and a. niger, and 20-30% larger than that of either a. nidulans or a. fumigatus. a. oryzae and a. flavus have exactly the same number of aspartic proteinase genes, of which each orthologous pair sh ...200818798040
isolation and characterization of an extracellular antimicrobial protein from aspergillus oryzae.a 17 kda antimicrobial protein was isolated from growth medium containing the filamentous fungus aspergillus oryzae by extracting the supernatants from the culture media, ion exchange chromatography on cm-sepharose, and c18 reverse-phase high-performance liquid chromatography. this antimicrobial protein, which we considered to be an extracellular antimicrobial protein from a. oryzae (exap-ao17), possessed antimicrobial activity but lacked hemolytic activity. the exap-ao17 protein strongly inhibi ...200818803391
construction of an aspergillus oryzae cell-surface display system using a putative gpi-anchored protein.a novel cell-surface display system was constructed in aspergillus oryzae. each of the five genes encoding the putative cell-wall-localized protein from the a. oryzae genome was cloned and these cell-surface anchor functions were examined by fusion to the c-terminal of the green fluorescent protein (gfp). using the mp1 and cwp proteins as anchor proteins, gfp signals were strongly observed on the cell surface of recombinant a. oryzae. when these proteins were used as anchor proteins for cell-sur ...200818813924
genomics of aspergillus oryzae: learning from the history of koji mold and exploration of its a time when the notion of microorganisms did not exist, our ancestors empirically established methods for the production of various fermentation foods: miso (bean curd seasoning) and shoyu (soy sauce), both of which have been widely used and are essential for japanese cooking, and sake, a magical alcoholic drink consumed at a variety of ritual occasions, are typical examples. a filamentous fungus, aspergillus oryzae, is the key organism in the production of all these traditional foods, and it ...200818820080
efficient production and partial characterization of aspartyl aminopeptidase from aspergillus oryzae.aspartyl aminopeptidase (dap) has a high degree of substrate specificity, degrading only amino-terminal acidic amino acids from peptides. therefore, attention is focused here on the efficient production of this enzyme by a recombinant aspergillus oryzae and characterization of its biochemical properties.200818828788
two types of beta-fructofuranosidases from aspergillus oryzae kb.aspergillus oryzae kb produces two types of beta-fructofuranosidases, f1 and f2. f1 produces 1-kestose, nystose, and fructosyl nystose from sucrose through its transfructosylation action. f2 hydrolyzes sucrose to glucose and fructose. n-terminal amino acid sequences of the purified enzymes were dynaappnlst for f1 and ysgdlrpq for f2. each enzyme encoding gene was identified in the genome of aspergillus oryzae. although the kb strain showed a higher production of f2 than f1 in a low sucrose liqui ...200818088091
corncob-induced endo-1,4-beta-d-xylanase of aspergillus oryzae mtcc 5154: production and characterization of xylobiose from glucuronoxylan.eight different fungi were cultivated in a peptone-yeast extract medium containing 1% oat spelt xylan (osx) to evaluate endo-1,4-beta-xylanase secretion for xylooligosaccharide (xos) production. aspergillus oryzae mtcc 5154, aspergillus flavus , aspergillus niger , and aspergillus ochraceus showed significant titers of endoxylanases, which were further used for the production of xos from birch wood xylan (bwx). a. oryzae produced 89.5 +/- 1.13% xos in the hydrolysate at 24 h of reaction. the eff ...200818489109
role of n-terminal 28-amino-acid region of rhizopus oryzae lipase in directing proteins to secretory pathway of aspergillus develop a new approach for improving heterologous protein production in aspergillus oryzae, we focused on the functional role of the n-terminal region of rhizopus oryzae lipase (rol). several n-terminal deletion variants of rol were expressed in a. oryzae. interestingly, a segment of 28 amino acids from the c-terminal region of the propeptide (n28) was found to be critical for secretion of rol into the culture medium. to further investigate the role of n28, the rol secretory process was visua ...200818496686
improved annotation through genome-scale metabolic modeling of aspergillus oryzae.since ancient times the filamentous fungus aspergillus oryzae has been used in the fermentation industry for the production of fermented sauces and the production of industrial enzymes. recently, the genome sequence of a. oryzae with 12,074 annotated genes was released but the number of hypothetical proteins accounted for more than 50% of the annotated genes. considering the industrial importance of this fungus, it is therefore valuable to improve the annotation and further integrate genomic inf ...200818500999
new, efficient synthesis of oseltamivir phosphate (tamiflu) via enzymatic desymmetrization of a meso-1,3-cyclohexanedicarboxylic acid diester.a new, enantioselective synthesis of the influenza neuraminidase inhibitor prodrug oseltamivir phosphate 1 (tamiflu) and its enantiomer ent-1 starting from cheap, commercially available 2,6-dimethoxyphenol 10 is described. the main features of this approach comprise the cis-hydrogenation of 5-(1-ethyl-propoxy)-4,6-dimethoxy-isophthalic acid diethyl ester (6a) and the desymmetrization of the resultant all-cis meso-diesters 7a and 7b, respectively. enzymatic hydrolysis of the meso-diester 7b with ...200818517254
high prevalence of fastidious bacteria in 1520 cases of uveitis of unknown etiology.the etiologic evaluation of uveitis is frequently unsuccessful when noninvasive methods are used. we conducted a prospective study to evaluate systematic screening for pathogens of uveitis. all patients with uveitis referred to the participating tertiary ophthalmology departments from january 2001 to september 2007 underwent intraocular and serum specimen collection. the standardized protocol for laboratory investigations included universal polymerase chain reaction (pcr)-based detection of any ...200818520326
enhancement of the alcoholytic activity of alpha-amylase amya from thermotoga maritima msb8 (dsm 3109) by site-directed mutagenesis.amya, an alpha-amylase from the hyperthermophilic bacterium thermotoga maritima, is able to hydrolyze internal alpha-1,4-glycosidic bonds in various alpha-glucans at 85 degrees c as the optimal temperature. like other glycoside hydrolases, amya also catalyzes transglycosylation reactions, particularly when oligosaccharides are used as substrates. it was found that when methanol or butanol was used as the nucleophile instead of water, amya was able to catalyze alcoholysis reactions. this capabili ...200818552192
dephosphorylation and quantification of organic phosphorus in poultry litter by purified phytic-acid high affinity aspergillus phosphohydrolases.extracellular phosphohydrolases mediate the dephosphorylation of phosphoesters and influence bioavailability and loss of agricultural p to the environment to pose risks of impairment of sensitive aquatic ecosystems. induction and culture of five strains of aspergillus were conducted to develop a source of high-affinity and robust phosphohydrolases for detecting environmental p and quantifying bioactive p pools in heterogeneous environmental specimens. enzyme stability and activity against organi ...200818555509
multiple gene disruptions by marker recycling with highly efficient gene-targeting background (deltaligd) in aspergillus oryzae.previously we reported that double disruption of the proteinase genes (tppa and pepe) improved heterologous protein production by aspergillus oryzae (jin et al. appl microbiol biotechnol 76:1059-1068, 2007). since a. oryzae has 134 protease genes, the number of auxotrophy in a single host is limited for multiple disruptions of many protease genes. in order to rapidly perform multiple gene disruptions in a. oryzae, we generated the marker recycling system in highly efficient gene-targeting backgr ...200818574559
optimization of ellagic acid production from ellagitannins by co-culture and correlation between its yield and activities of relevant enzymes.aspergillus oryzae was co-cultured with trichoderma reesei using acorn cups extract containing up to 62% ellagitannins as substrate to produce ellagic acid with relatively high levels of ellagitannin acyl hydrolase, cellulase and xylanase. ellagitannins concentration, initial ph, t. reesei and a. oryzae during the fermentation were identified as important process parameters effecting ellagic acid accumulation and the enzymes syntheses. these parameters were optimized by uniformity design to dete ...200817363241
physico-chemical and microbiological analyses of fermented corn cob, rice bran and cowpea husk for use in composite rabbit experiment was conducted to evaluate the effect of fermentation on the proximate composition of corn cob, rice bran and cowpea husk for use in composite rabbit feed formulations. the test ingredients were moistened with tap water and allowed to ferment naturally at room temperature. during fermentation, samples of the fermenting materials were extracted at zero, 24 and 48 h for physico-chemical and microbiological analyses using standard procedures. the microorganisms associated with the ferm ...200817502134
utilization of acorn fringe for ellagic acid production by aspergillus oryzae and endomyces fibuliger.conversion of acorn fringe extract into ellagic acid production by aspergillus oryzae and endomyces fibuliger were investigated. the results showed that ellagic acid production was maximized when co-fermentation of the two fungi was performed at 30 degrees c and ph 5.0 with 5.7 g/l of initial substrate concentration, which were close to the optimal values for both fungi to yield an appropriate consortium of hydrolytic enzymes. meanwhile, it was found that the co-fermentation could compensate the ...200817826988
utilization of agricultural residues for poly(3-hydroxybutyrate) production by halomonas boliviensis lc1.utilization of cheap and readily available agricultural residues as cheap carbon sources for poly(3-hydroxybutyrate) (phb) production by halomonas boliviensis.200817887984
production of fungal biomass protein using microfungi from winery wastewater treatment.this study was carried out to investigate the production of fungal biomass protein (fbp) in treatment of winery wastewater using microfungi. three fungal strains, trichoderma viride webl0702, aspergillus niger webl0901 and aspergillus oryzae webl0401, were selected in terms of microbial capability for fbp production and cod reduction. t. viride appeared to be the best strain for fbp production due to high productivity and less nitrogen requirement. more than 5 g/l of fungal biomass was produced ...200817911010
inhibitory effects of fermented brown rice on induction of acute colitis by dextran sulfate sodium in rats.although the pathogenic mechanisms of inflammatory bowel diseases are not fully understood, colonic microbiota may affect the induction of colonic inflammation, and some probiotics and prebiotics have been reported to suppress colitis. the inhibitory effects of brown rice fermented by aspergillus oryzae (fbra), a fiber-rich food, on the induction of acute colitis by dextran sulfate sodium (dss) were examined. feeding a 5% and 10% fbra-containing diet significantly decreased the ulcer and erosion ...200817957470
purification and biochemical characterization of a thermostable extracellular glucoamylase produced by the thermotolerant fungus paecilomyces extracellular glucoamylase produced by paecilomyces variotii was purified using deae-cellulose ion exchange chromatography and sephadex g-100 gel filtration. the purified protein migrated as a single band in 7% page and 8% sds-page. the estimated molecular mass was 86.5 kda (sds-page). optima of temperature and ph were 55 degrees c and 5.0, respectively. in the absence of substrate the purified glucoamylase was stable for 1 h at 50 and 55 degrees c, with a t (50) of 45 min at 60 degrees c. th ...200817938981
transcriptional regulation of genes on the non-syntenic blocks of aspergillus oryzae and its functional relationship to solid-state cultivation.transcriptome analysis revealed close relationship between solid-state cultivation and the transcriptional regulation of the genes on the non-syntenic blocks (nsbs), which were characterized by the comparison of aspergillus oryzae genome with those of aspergillus fumigatus and aspergillus nidulans. average expression ratio of the genes on nsbs in solid-state cultivation was significantly higher than that on the syntenic blocks (sbs). of the induced genes, the genes relating to metabolism, which ...200817967552
multivariate models for prediction of rheological characteristics of filamentous fermentation broth from the size distribution.the main purpose of this article is to demonstrate that principal component analysis (pca) and partial least squares regression (plsr) can be used to extract information from particle size distribution data and predict rheological properties. samples from commercially relevant aspergillus oryzae fermentations conducted in 550 l pilot scale tanks were characterized with respect to particle size distribution, biomass concentration, and rheological properties. the rheological properties were descri ...200818023062
diastereoselective synthesis of (r)-(alkyl)-beta-d-galactopyranoside by using beta-galactosidase (aspergillus oryzae) in low-water media.a beta-galactosidase (from aspergillus oryzae) preparation viz. eprp (enzyme precipitated and rinsed with propanol), obtained by the removal of bulk water by precipitation with n-propanol, showed higher biological activity than the lyophilized powder. ft-ir study confirmed that eprp had retained the alpha-helical content of the native structure better than the lyophilized form. use of this formulation of beta-galactosidase under low water conditions (temperature 55 degrees c, reaction time of 4 ...200818024031
solid-state fermentation with fungi to enhance the antioxidative activity, total phenolic and anthocyanin contents of black the present study, solid-state fermentation of black bean with various gras filamentous fungi including aspergillus awamori, aspergillus oryzae, aspergillus sojae, rhizopus azygosporus and rhizopus sp. no. 2 was preformed to prepared koji. mycelial propagation of starter organisms and antioxidative activity, including alpha-alpha-diphenyl-2-picyl-hydoxyl (dpph) radicals, fe2+-chelating ability, and reducing activity, were examined. depending upon the starter organism, various amounts of mycel ...200818031859
entrapment of beta-galactosidase in polyvinylalcohol hydrogel.beta-galactosidase isolated from aspergillus oryzae was immobilized in lens-shaped polyvinylalcohol capsules (with activity 25 u g(-1)) giving 32% of its original activity. immobilization did not change the ph optimum (4.5) of lactose hydrolysis. the relative enzyme activity during product inhibition testing was, in average, 10% higher for immobilized enzyme. no decrease of activity was observed after 35 repeated batch runs and during 530 h of continuous hydrolysis of lactose (10%, w/v) at 45 de ...200818043870
physicochemical characterization of fructooligosaccharides and evaluation of their suitability as a potential sweetener for diabetics.fructooligosaccharides (foss) were prepared from sucrose using fungal fructosyl transferase (ftase) obtained from aspergillus oryzae mtcc 5154. the resulting mixture consisted of glucose (28-30%), sucrose (18-20%) and fructooligosaccharides (50-54%) as indicated by hplc analysis. identification of oligomers present in the mixture of fructooligosaccharides was carried out using nmr spectroscopy and lc-ms. no compounds other than mono-, di-, tri-, tetra- and pentasaccharides were identified in the ...200818005951
immunoreactivity and amino acid content of fermented soybean allergy has become a public health problem that continues to challenge both the public and the food industry. the objective of this research was the detection and quantification of the major human allergenic soy proteins and to study the reduction in immunoreactivity and improvement of amino acid content after fermentation of soybean flour. fermentation was carried out in the solid state of cracked seeds inoculated with aspergillus oryzae, rhizopus oryzae, and bacillus subtilis and in the l ...200818072744
identification of ace-inhibitory peptides in salt-free soy sauce that are transportable across caco-2 cell present study, we aimed to identify angiotensin i-converting enzyme (ace)-inhibitory peptides from a salt-free soy sauce (sfs), a newly developed antihypertensive seasoning obtained by aspergillus oryzae fermentation of soybean in the absence of salt, which can be transported through caco-2 cell monolayers. through an ussing transport investigation of sfs across caco-2 cell monolayers, three di-peptides, ala-phe, phe-ile and ile-phe, were successfully identified from the sfs as transportable ...200818160180
deletion analysis of the catalase-encoding gene (catb) promoter from aspergillus oryzae.the catalase-encoding gene (catb) is expressed strongly in aspergillus oryzae. to identify the transcription regulatory elements involved in strong expression, we did promoter deletion analysis using beta-glucuronidase (gus) as a reporter and an electrophoretic gel mobility shift assay (emsa) systematically. the deletion 200-bp sequence from -1,000 to -800 in the 1,400-bp catb promoter caused a drastic decrease in gus activity. in addition, emsa implicated a 45-bp element from -1,000 to -956 con ...200818175923
application of a statistical design to the optimization of parameters and culture medium for alpha-amylase production by aspergillus oryzae cbs 819.72 grown on gruel (wheat grinding by-product).the production optimization of alpha-amylase (e.c. from aspergillus oryzae cbs 819.72 fungus, using a by-product of wheat grinding (gruel) as sole carbon source, was performed with statistical methodology based on three experimental designs. the optimisation of temperature, agitation and inoculum size was attempted using a box-behnken design under the response surface methodology. the screening of nineteen nutrients for their influence on alpha-amylase production was achieved using a pla ...200818180155
fungal mycelia show lag time before re-growth on endogenous carbon.nutrient starvation is a common occurrence for filamentous fungi. to better understand the effects of starvation, we used a parallel plate flow chamber to study individual fungal mycelia when subjected to a step change in glucose concentration. we report the presence of a finite "lag time" in starved mycelia during which they ceased to grow/extend while switching from growth on exogenous carbon to re-growth on endogenous carbon. this lag time precedes other morphological or physiological changes ...200818183629
high-level expression, purification and characterization of recombinant aspergillus oryzae alkaline protease in pichia pastoris.the alkaline protease gene from aspergillus oryzae was cloned, and then it was successfully expressed in the heterologous pichia pastoris gs115 with native signal peptide or alpha-factor secretion signal peptide. the yield of the recombinant alkaline protease with native signal peptide was about 1.5-fold higher than that with alpha-factor secretion signal peptide, and the maximum yield of the recombinant alkaline protease was 513 mg/l, which was higher than other researches. the recombinant alka ...200818226921
effect of cultivation ph and agitation rate on growth and xylanase production by aspergillus oryzae in spent sulphite liquor.the effects of cultivation ph and agitation rate on growth and extracellular xylanase production by aspergillus oryzae nrrl 3485 were investigated in bioreactor cultures using spent sulphite liquor (ssl) and oats spelts xylan as respective carbon substrates. xylanase production by this fungus was greatly affected by the culture ph, with ph 7.5 resulting in a high extracellular xylanase activity in the ssl-based medium as well as in a complex medium with xylan as carbon substrate. this effect, th ...200818239946
monitoring global gene expression of proteases and improvement of human lysozyme production in the nptb gene disruptant of aspergillus oryzae.aspergillus oryzae has numerous protease genes that might cause proteolytic degradation of heterologously-produced proteins. the productivity of the heterologous protein can be improved by protease gene disruption, but it is difficult to select disruption targets efficiently. in this study, we monitored the expression of 132 protease genes by dna microarray. a group of protease genes up-regulated during cultivation was identified by clustering analysis. in this protease group, the nptb gene enco ...200818256487
authentic heterologous expression of the tenellin iterative polyketide synthase nonribosomal peptide synthetase requires coexpression with an enoyl reductase.the tens gene encoding tenellin synthetase (tens), a 4239-residue polyketide synthase nonribosomal-peptide synthetase (pks-nrps) from beauveria bassiana, was expressed in aspergillus oryzae m-2-3. this led to the production of three new compounds, identified as acyl tetramic acids, and numerous minor metabolites. consideration of the structures of these compounds indicates that the putative c-terminal thiolester reductase (r) domain does not act as a reductase, but appears to act as a dieckmann ...200818266306
a defect of ligd (human lig4 homolog) for nonhomologous end joining significantly improves efficiency of gene-targeting in aspergillus oryzae.gene-targeting by homologous recombination occurs rarely during transformation since nonhomologous recombination is predominant in aspergillus oryzae. to develop a highly efficient gene-targeting system for a. oryzae, we constructed disrupted strains harboring a gene (ligd) encoding human dna ligase iv homolog that is involved in the final step of dna nonhomologous end joining. the a. oryzae ligd disruptants showed no apparent defect in vegetative growth and/or conidiation, and exhibited increas ...200818282727
alcoholic chestnut fermentation in mixed culture. compatibility criteria between aspergillus oryzae and saccharomyces cerevisiae strains.the main objective of the present work consisted in the transfer to the case of the chestnut of a rice fermentative process that carried out to the japanese traditional way to lead to an alcoholic bagasse, the moromi, capable of obtaining distilled. this way, selection assays of amylolitic aspergillus oryzae strains and studies of compatibility between microfungi and yeast were carried out. these mixed cultivations were performed operating in batch submerged culture. later on, using solid state ...200818289846
cloning and characterization of the glyceraldehyde-3-phosphate dehydrogenase gene and the use of its promoter for expression in myrothecium gramineum, a novel expression our laboratory, research has focused on the development of myrothecium gramineum as a novel expression host. the glyceraldehyde-3-phosphate dehydrogenase (gpd)-promoter of m. gramineum was isolated and characterized (genbank accession number ef486690). in order to prove its functionality and to explore the potential of m. gramineum as a novel fungal expression host, use of this gpd-promoter for the expression of a fungal alpha-amylase was investigated. myrothecium gramineum was transformed wi ...200818294194
fusarielin a as an anti-angiogenic and anti-proliferative agent: basic biological characterization.fusarielin a shows anti-angiogenic activity in the human umbilical vein endothelial cell (huvec) tube formation assay. structural development studies indicated the importance of the hydroxyl groups in this molecule. a 3h-labeled derivative and a fluorescent affinity-labeling agent were prepared and used to examine the cellular distribution and biological behavior of fusarielin a.200818310939
methylation in methanol-water mixtures: the effect of solvent composition and high pressure.the effect of pressure (0.1 to 400 mpa) and solvent composition (methanol concentration of 5 to 30%) on the synthesis of beta-methylgalactoside was studied. galactose was used as a reactant and the reaction was catalyzed by beta-galactosidase from aspergillus oryzae. under the applied conditions the enzyme was sufficiently stable and the reaction equilibrium was reached. higher methanol concentrations obviously influenced the product yield positively due to an increase in reactant concentration ...200818329783
a trispecies aspergillus microarray: comparative transcriptomics of three aspergillus species.the full-genome sequencing of the filamentous fungi aspergillus nidulans, aspergillus niger, and aspergillus oryzae has opened possibilities for studying the cellular physiology of these fungi on a systemic level. as a tool to explore this, we are making available an affymetrix genechip developed for transcriptome analysis of any of the three above-mentioned aspergilli. transcriptome analysis of triplicate batch cultivations of all three aspergilli on glucose and xylose media was used to validat ...200818332432
riboswitches for enhancing target gene expression in eukaryotes. 200818338354
isoflavone aglycones production from isoflavone glycosides by display of beta-glucosidase from aspergillus oryzae on yeast cell surface.for efficient production of isoflavone aglycones from soybean isoflavones, we isolated three novel types of beta-glucosidase (bgl1, bgl3, and bgl5) from the filamentous fungi aspergillus oryzae. three enzymes were independently displayed on the cell surface of a yeast saccharomyces cerevisiae as a fusion protein with alpha-agglutinin. three beta-glucosidase-displaying yeast strains hydrolyzed isoflavone glycosides efficiently but exhibited different substrate specificities. among these beta-gluc ...200818340445
a microtitre plate assay for measuring glycosidase activity.glycosidases perform a wide range of functions in physiology and pathology, and are potential targets for the treatment of diseases such as influenza, cancer, aids and diabetes. this paper reports a convenient discontinuous colourimetric assay for the measurement of glycosidase activity. the assay utilises 4-nitrophenyl- substrates and quantities of product are determined by measuring absorbance at 405 nm. this assay is performed in a 96 well microtitre plate and has been used to characterise th ...200818341264
a highly efficient gene-targeting system for aspergillus establish a system that greatly increases the gene-targeting frequency in aspergillus parasiticus.200818346134
acid activation of protyrosinase from aspergillus oryzae: homo-tetrameric protyrosinase is converted to active dimers with an essential intersubunit disulfide bond at acidic ph.aspergillus oryzae protyrosinase (pro-ty) has a unique feature that the proenzyme is activated under conditions of acidic ph. the pro-ty was inactive at ph 7.0. the latent enzyme was activated at ph 3.0, and was slightly activated by sodium dodecyl sulfate (sds). the molecular masses of the pro-ty and acid-activated tyrosinase (acid-ty) were 266 and 165 kda, respectively, as estimated by gel-filtration chromatography. the cd spectra showed that the tertiary and/or quaternary structure was change ...200818353147
gene structure dynamics and divergence of the polygalacturonase gene family of plants and fungus.whole copies of the polygalacturonase (pg) genes from rice (oryza sativa subsp. japonica) and a filamentous fungus (aspergillus oryzae) were isolated. the orthologs of the rice pgs were also retrieved from other plant species. the 106 plant pgs analyzed were divided into 5 clades, a, b, c, d, and e. the fungus pgs were classified into 3 clades, of which one formed a loose cluster with clade e of the plant pgs. four domain motifs (i, ii, iii, iv) were identified in all pgs. motifs ii and iii were ...200818356937
purification of alpha-galactosidase and invertase by three-phase partitioning from crude extract of aspergillus oryzae.alpha-galactosidase and invertase were accumulated in a coherent middle phase in a three-phase partitioning system under different conditions (ammonium sulphate, ratio of tert-butanol to crude extract, temperature and ph). alpha-galactosidase and invertase were purified 15- and 12-fold with 50 and 54% activity recovery, respectively. the fractions of interfacial precipitate arising from the three-phase partitioning were analyzed by sds-page. both purified preparations showed electrophoretic homo ...200818414794
a wheat biorefining strategy based on solid-state fermentation for fermentative production of succinic this study, a novel generic feedstock production strategy based on solid-state fermentation (ssf) has been developed and applied to the fermentative production of succinic acid. wheat was fractionated into bran, gluten and gluten-free flour by milling and gluten extraction processes. the bran, which would normally be a waste product of the wheat milling industry, was used to produce glucoamylase and protease enzymes via ssf using aspergillus awamori and aspergillus oryzae, respectively. the r ...200818434138
cloning and expression of a gene encoding gallerimycin, a cysteine-rich antifungal peptide, from eri-silkworm, samia cynthia ricini.a cdna clone encoding gallerimycin was isolated from larval fat body of immunized samia cynthia ricini and named as scr-gallerimycin. in naive larvae, no gene expression was detected, but strongly induced in fat body and hemocytes following immune challenge with bacteria or entomopathogenic fungus beauveria bassiana. strong expression of the gene was also induced by injection of peptidoglycan and zymosan, but very weakly by non-pathogenic fungus aspergillus oryzae. analysis of the sequence upstr ...200818436462
concanavalin a layered calcium alginate-starch beads immobilized beta galactosidase as a therapeutic agent for lactose intolerant patients.a novel therapeutic agent in the form of beta galactosidase immobilized on the surface of concanavalin a layered calcium alginate-starch beads has been developed. immobilized beta galactosidase exhibited significantly very high stability against conditions of digestive system such as ph, salivary amylase, pepsin and trypsin. soluble and immobilized beta galactosidase exhibited same ph-optima. however, the immobilized enzyme retained greater fraction of catalytic activity at higher and lower ph t ...200818439774
lactic fermentation of cellobiose by a yeast strain displaying beta-glucosidase on the cell surface.the aspergillus aculeatus beta-glucosidase 1 (bgl1) gene was expressed in a lactic-acid-producing saccharomyces cerevisiae strain to enable lactic fermentation with cellobiose. the recombinant beta-glucosidase enzyme was expressed on the yeast cell surface by fusing the mature protein to the c-terminal half region of the alpha-agglutinin. the beta-glucosidase expression plasmids were integrated into the genome. three strong promoters of s. cerevisiae, the tdh3, pgk1, and pdc1 promoters, were use ...200818443785
aspergillus oryzae atfb encodes a transcription factor required for stress tolerance in conidia.using an aspergillus oryzae est database, we identified a gene encoding a transcription factor (atfb), which is a member of the atf/creb family. expression of atfb was barely detectable during vegetative growth, but was readily detected during conidiation in solid-state culture. microarray analyses showed that expression of many other genes, including catalase (cata), were downregulated in an atfb-disruptant. the expression of most of these genes was upregulated in the wild-type strain during th ...200818448366
enzymatic production of epigallocatechin by using an epigallocatechin gallate hydrolase induced from aspergillus oryzae.catechins are a group of polyphenolic compounds that are antioxidants having beneficial biological activities. there are four main catechins in green tea, and each has its own biological features. in order to fully exploit prominent biological activities of specific catechins and to develop new medicine from catechins, it is necessary to obtain pure catechin preparations by isolation from natural sources, by chemical synthesis, or by biotransformation reactions with high yield and specificity. i ...200818452340
[polarized distribution of intracellular organelles involved in vesicular trafficking in filamentous fungal cells]. 200818453152
efficient and direct fermentation of starch to ethanol by sake yeast strains displaying fungal glucoamylases.aspergillus oryzae glucoamylases encoded by glaa and glab, and rhizopus oryzae glucoamylase, were displayed on the cell surface of sake yeast saccharomyces cerevisiae gri-117-uk and laboratory yeast s. cerevisiae mt8-1. among constructed transformants, gri-117-uk/pudgaa, displaying glaa glucoamylase, produced the most ethanol from liquefied starch, although mt8-1/pudgar, displaying r. oryzae glucoamylase, had the highest glucoamylase activity on its cell surface.200818460787
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