TitleAbstractYear(sorted ascending)
[isolation of fractions having aminopeptidase activity (ec (arylamidase) from culture medium of aspergillus oryzae]. 19744447798
[the effect of sodium chloride and sulfate on the growth and amylolytic activity of the fungus aspergillus oryzae]. 19744449783
[biosynthesis of beta-fructofuranosidase by fungi of the genus aspergillus]. 19744463361
[free intracellular amino acids of aspergillus oryzae mycelia and acid proteinase synthesis]. 19744464877
further evidence on differentiation of aspergillus sojae from aspergillus oryzae by electrophoretic patterns of cellulase, pectin-lyase, and acid proteinase. 19744822056
single-stranded regions in dna isolated from different developmental stages of the sea urchin.long-term labeled sea urchin embryo (strongylocentrotus purpuratus) dnas were examined for size of recovered pieces, single-strandedness, and length of continuous double-stranded regions. sizing on neutral sucrose gradients indicates that morula stage dna sediments predominantly at 31 s, blastula stage dna at 27 s, and gastrula stage dna as a broad range of sizes of greater than 29 s. treatment of [3h]thymidine-labeled dna with aspergillus oryzae s1 nuclease removes 19% of the 3h from morula sta ...197411400427
isolation of a short, cytosine-rich repeating unit from the dna of escherichia coli.hybridization of heterologous nucleic acids has provided the means for isolating a repeating sequence which is located next to template regions of dna. separated single strands of 32p-labelled dna from escherichia coli were to a limited extent able to anneal with dna of micrococcus lysodeikticus immobilized on nitrocellulose membrane filters. the resulting hybrid was resistant to enzymes specific for unpaired strands, nuclease s1 (aspergillus oryzae) and exonuclease i (e. coli). the e. coli dna ...197411400431
purification and properties of beta-galactosidase from aspergillus oryzae.beta-galactosidase [ec] has been purified from a culture of aspergillus oryzae by 2-propanol fractionation, column chromatography on deae-sephadex a-50 and sephadex g-200. the preparation was homogeneous on ultracentrifugation and disc electrophoresis. the enzyme showed ph optima of 4.5 with onpg-1 as a substrate and 4.8 with lactose as a substrate. the stable ph range was from 4.0 to 9.0 and the optimum temperature was 46 degrees. the michaelis constants were 7.2 x 10-minus 4 m with on ...1975236999
aspergillus oryzae acid proteinase. purification and properties, and formation of acid proteinase from aspergillus oryzae was isolated from a commercial powder by successive (nh4)2so4 fractionation, acetone precipitation, and ion-exchange chromatography on phosphate- and deae-cellulose columns. the purified enzyme was found to be homogeneous by ultracentrifuge-sedimentation analysis (s20, w equal 3.63s), but electrofocusing in polyacrylamide gels and electrophoresis at ph 3.2 revealed that it consists of two very closely migrating bands. no difference in the amino acid com ...1975239702
[studies on the reaction mechanism of a ribonuclease ii from aspergillus oryzae (author's transl)].ribonuclease t2 was isolated from an aspergillus oryzae extract. in order to define the substrate specificity, the hydrolysis of a series of 2',3'-cyclic nucleotides was measured semiquantitatively. modifications in all positions of the bases are tolerated, as long as the base stays in the anti conformation or has a chance to return to it; bulky substituents at n-3 of the pyrimidine base lower the rate. so far the conclusion seems justified that the enzyme does not react with the substrates by s ...1975240766
on the physical properties and mechanism of action of arylsulfate sulfohydrolase ii from aspergillus oryzae. 1975241293
activity of endonuclease s1 in denaturing solvents: dimethysulfoxide, dimethylformamide, formamide and formaldehyde. 1975241350
purification and characterization of extracellular proteinases of aspergillus oryzae.the extracellular proteinases of aspergillus oryzae ei 212 were separated into two active fractions by (nh4)2so4 and ethanol fractionation followed by diethylaminoethyl-sephadex a-50 and hydroxyapatite chromatography. the molecular weight was estimated by gel filtration to be about 70,000 and 35,000 for proteinases i and ii, respectively. optimum ph for casein and hemoglobin hydrolysis was 6.5 at 60 c for proteinase i and 10.0 at 45 c for proteinase ii, and for gelatin hydrolysis it was 6.5 at 4 ...1975242254
histamine induction and release following proteolytic enzyme exposure.the mechanism of biologic response from exposure to a 12% subtilisin carlsberg preparation is shown to be one of histamine release in the guinea pig. three groups of guinea pigs were pretreated by intradermal injections withsaline solution of (1) the commercial proteolytic enzyme preparation containing 12% subtilisin carlsberg, (2) an alkaline protease preparation obtain from aspergillus oryzae that was isolated from cotton dust, or (3) a nonproteolytic mixture of proteins and lipases obtained f ...197548332
variations in hybridization of rna from different mouse tissues and embryos to endogenous c-type virus dna transcripts.several adult tissues, newborns, and embryos of uninfected balb/c mice were analysed for rna complementary to [3h]-dna transcripts synthesized from an endogenous type-c virus of balb/c 3t3. the technique of rna:dna hybridization was used and the extent of hybridization was measured by the use of a single-strand-specific nuclease (s-i), purified from aspergillus oryzae. virus-specific rna was detected in all adult and embryonic tissues tested. however, the rna extracted from tissues having higher ...1975169317
specificity of the s1 nuclease from aspergillus oryzae.conditions are described for digesting single-stranded dna by s1 nuclease without introducing breaks in double-stranded dna. the enzyme is inhibited by low concentrations of various compounds of phosphate. under certain conditions s1 nuclease cleaves the strand opposite a nick in bacteriophage t5 dna; under other conditions, the enzyme cleaves a loop in one strand of heteroduplex lambdadna while leaving the opposite strand intact. s1 nuclease makes many single strand breaks in ultraviolet-irradi ...1975171268
the capacity of alpha-amylases to catalyze the nonhydrolytic degradation of starch and glycogen with formation of novel glycosylation products. 1975810091
amino acid analogs. iii: new syntheses of monomethyl- and monophenylglutamic acids.glutamic acid analogs containing 3- and 4-methyl and 2-, 3-, and 4-phenyl substituents were prepared. the 3- and 4-methyl- and 3- and 4-phenylglutamic acids did not inhibit plasmodium berghei and were nontoxic to the host (mice) at 640 mg/kg. the five analogs in addition to 2-methlglutamic acid were inactive against lactobacillus casei at 1000 mug/ml in a defined medium: against escherichia coli, only 2-methylglutamic acid caused 27% inhibition at 10,000 mug/ml. all six analogs failed to inhibit ...1975811786
thrombolytic treatment with i.v. brinase of advanced arterial obliterative disease of the limbs.the material includes 17 patients suffering from different degrees of chronic peripheral arterial disease (11 chronic patients stage iii and iv, two patients with acute arterial occlusion, and four patients stage ii). presence and extent of arterial occlusion was ascertained by initial arteriography. in twelve of the patients amputation had been considered. the patients were treated by a series of i.v. infusions of brinase, a proteolytic enzyme from aspergillus oryzae. the brinase inhibitor capa ...19751053595
comparison of the effects of linear and cyclic phosphates on the adsorption of proteins by human enamel.treatment of human enamel powder with cyclic trimetaphosphate or linear tripolyphosphate changes subsequent adsorption of amylase, lysozyme, and salivary protein from aqueous solutions. treated enamel samples adsorb more lysozyme, less amylase, and more salivary protein (at concentrations exceeding 12 mug/ml) than controls treated with distilled water.19751056353
transglycosylation by the exo-type of beta-n-acetyglucosaminidase from human parotid saliva. 19751057392
the enhancing influence of proteolysis on e rosette forming lymphocytes (t cells) in vivo and in vitro.the t lymphocytes populations of 22 young healthy adults, 21 healthy middle aged and older blood donors, 35 non-pregnant women of child bearing age and 14 patients with advanced malignant disease were assessed and compared. it was found that the mean t cell counts in the middle aged and older controls were significantly lower than in the healthy young adults and were further reduced in the patients with malignant disease. the addition of the proteolytic agent brinase (protease 1 obtained from as ...19751080669
investigation into the use of aspergillus oryzae s1 nuclease in the presence of solvents which destabilize or prevent dna secondary structure: formaldehyde, formamide, and glyoxal. 19751093442
fatal aspergillosis in imported parrots.spontaneous fatal aspergillosis occurred in several species of parrots imported from latin america, australia, malaya and ghana for studies on the control of psittacosis. over a period of 4 years, 655 parrots were imported for use in these studies. all birds that died during these investigations were necropsied, and the internal organs of 45 were found to have macroscopic lesions suggestive of aspergillosis. of these 45 suspected cases, 32 were confirmed as aspergillosis by both histopathology a ...19751097931
reversal of fungitoxicity of 8-quinolinols and their copper (ii) bischelates. i. amino acids and related compounds.the effect of amino acids and related compounds on the toxicity of 8-quinolinols and their copper (ii) bischelates to aspergillus oryzae (atcc 1011) was studied. none of the compounds tested except the thiol-containing compounds, cysteine, cysteamine, glutathione, and n-acetylcysteine reversed the inhibitory action of 8-quinolinol but not that of 5-iodo-8-quinolinol or bis (8-quinolinolato) copper (ii). it appears that the mechanism(s) of fungitoxicity of 8-quinolinol is different from that of 5 ...19751116048
specific hydrolysis of the cohesive ends of bacteriophage lambda dna by three single strand-specific nucleases.procedures have been worked out for aspergillus nuclease s1 and mung been nuclease to quantitatively cleave off both of the 12-nucleotide long, single-stranded cohesive ends of lambdadna. this cleavage is indicated by the almost complete elimination of the repair incorporation of radioactive nucleotides by dna polymerase into the digested dna. with s1 nuclease, cleavage was complete at 10 degrees as well as at 30 degrees. under the conditions for quantitative cleavage of the single-stranded regi ...19751141222
transformation of malathion by a fungus, aspergillus oryzae, isolated from a freshwater pond. 19751148416
[action of leucine aminopeptidase 1 and 2 (aspergillus oryzae 410) on various synthetic peptides (author's transl)].by use of di- or tripeptides as substrates, lap 1 and lap 2, 2 fractions from aspergillus oryzae hydrolyze oligopeptides that possess leucine as n-terminal amino acid. lap 1 fraction also hydrolyzes the histidyl bond. both fractions have no activity towards peptides as glutathion, gly-pro-ala; they have low or no activity towards tyrosyl and tryptophanyl bond.19751157844
triacetonamine formation in fungal extracts. 19751159172
in vitro recognition of carcinogen-induced local denaturation sites native dna by s1 endonuclease from aspergillus oryzae. 19751161018
purification and some properties of the soluble and membrane-bound adenosine deaminases of micrococcus sodonensis atcc 11880 and their distribution within the family micrococcus sodonensis and some other micrococcus species, adenosien deaminase is present both as a membran-bound and a soluble enzyme; the membran-bound adenosine deaminase can be extracted with n-butanol, and may account for up to 5% of the total cellular adenosine deaminase activity. in a number oc comparative tests, no differences between the two enzyme forms could be found, thus they are believed to be similar molecular species; the purified membran-bound or soluble enzyme had a molecula ...19751168529
[growth patterns of fungi]. 19751169575
a chemical and biological assessment of aspergillus oryzae and other filamentous fungi as protein sources for simple stomached animals. 19751172167
s1 nuclease hydrolysis of single-stranded nucleic acids with partial double-stranded configuration.the single-strand specific nuclease s1 from aspergillus oryzae (ec was purified 600-fold in 16% yield from dried mycelia. determination of the isoelectric point of s1 nuclease as 4.3-4.4 allowed adjustment of chromatographic conditions such that the enzyme was isolated free of contaminating ribonucleases t1 and t2. s1 nuclease so purified was used for removal of single-stranded portions from the rna of the escherichia coli phage ms2, which has a helical content of about 65% in vitro. a ...19751182098
studies on the arylsulphatase and phenol sulphotransferase activities of aspergillus oryzae.1. crude extracts of aspergillus oryzae grown under conditions of sulphur limitation possess high arylsulphatase activity. 2. this activity can be greatly enhanced by the inclusion of tyramine or a number of other phenols in the assay medium. 3. the arylsulphatase activity of these extracts can be resolved into three distinct fractions by chromatography on deae-cellulose. 4. the effect of tyramine is restricted to one of these fractions only. 5. evidence is presented which indicates that this ef ...19751200998
the atmospheric fungal flora of the athens metropolitan the research programme of the department of microbiology of the athens university the nature of the mycological flora of the athenian air was studied. the research took place during the calendar year 1971. the open air was sampled twice weekly from two observation stations. the open plate technique was used, petri dishes containing sabourand's agar being exposed for 15 minutes. a total of 180 plates were exposed, and 1714 fungal colonies were isolated; these were subcultured and identified as ...19751207717
[denaturation stabilization of alpha-amylase from aspergillus oryzae].denaturation of alpha-amylase from aspergillus oryzae was studied under the effect of heating urea and some other denaturating agents. inhibition in the enzyme denaturation, deviation from the first order equation and, consequently, establishment of the false equilibrium in the system are shown. the values are calculated for the reaction rate constants of alpha-amylase denaturation under the effect to heat (40 degrees c) and urea. a method is developed for isolating native amylase stabilized by ...19751209773
[the amino acid sequence of the double-headed protein proteinase inhibitor from dog submandibular glands, i. structural homology to the pancreatic secretory trypsin inhibitors (author's transl)].the primary structure of the broad specificity proteinase inhibitor from dog submandibular glands was elucidated. the inhibitor consists of a single polypeptide chain of 117 amino acids which is folded into two domains (heads) connected by a peptide of three amino acid residues. both domains i and ii show a clear structural homology to each other as well as to the single-headed pancreatic secretory trypsin inhibitors (kazal type). the trypsin reactive site (-cys-pro-arg-leu-his-glx-pro-ile-cys-) ...19751213678
[effect of iaa on the content of rna in the cells and isolated nuclei of aspergillus oryzae].the content of rna in mycelium and isolated nuclei of aspergillus oryzae 3-9-15 was studied after its growth during two days on the modified capek medium containing 0.01 and 10 mg% of iaa. a direct correlation has been established between the extracellular concentration of iaa and its content in the mycelium and isolated nuclei of the fungus. the stimulating effect of the extracellular iaa on the content of rna in the isolated nuclei was optimal after one hour of incubation in the capek medium c ...19751214614
[chymotrypsin-like proteinases in microbial proteolytic complexes].a considerable amount of proteinase of chemotrypsin type (ao-ct) was found in the proteolytic system of asp. oryzae, strain "h"-476. the proteinase produces an intensive effect when hydrolyzing p-nitrophenyl acetate (p-npa) -- 18-20 times as strong as that of crystalline chemotrypsin. comparison of the ao-ct and chemotrypsin properties resulted in proving the enzyme nature of the p-npa hydrolysis reaction. a study of ao-ct specificity with the presence of such substrates as p-npa, o-nitro-phenyl ...19751216345
studies on the substrate specificity of taka-amylase a. xii. investigation of the active site of taka-amylase a by examining the properties of p-phenylazobenzoyl taka-amylase a.1. when p-phenylazobenzoyl taka-amylase a (phab-taa) was incubated at ph 6.5 with hydroxylamine for 3 hr at 20degrees, some of the p-phenylazobenzoyl residues that had been introduced into taka-amylase a (taa) [1, 4-alpha-d-glucan glucanohydrolase, ec, aspergillus oryzae] were liberated as a hydroxamic acid, and the activity pattern of phab-taa changed to that of intact taa. this result suggested that the p-phenylazobenzoyl residues liberated had been bound to the tyrosyl residue located ...19751225912
chemical mutagenesis. ii. some observations on the new morphogenetic variations induced in aspergillus oryzae wehmer by 6-azauracil. 19751242247
protein and amino acid changes in peanut (arachis hypogaea l.) seeds infected with aspergillus oryzae. 19761245677
renaturation of bacteriophage lambda dna. determination of the optimal renaturation conditions using a single-strand-specific dnase and alkaline-sucrose-gradient assay system.reannealed hybrid molecules of wild-type bacteriophage lambda dna were prepared in aqueous solutions of formamide at a variety of nacl concentrations at both room temperature ( 22 degrees c) and 37 degrees c. treatment of the hybrid dna molecules with the single-strand-specific nuclease s1 from aspergillus oryzae followed by alkaline sucrose gradient sedimentation was used to monitor the extent and fidelity of hybridization. the optimal renaturation conditions at room temperature were found to b ...19761248479
the in situ formation of dna-dna duplexes of drosophila virilis satellite dna.the dna of drosophila virilis brains and imaginal discs was labeled in vitro to a specific activity of 6 x 10(-5) dpm/mug, using an organ culture medium. the dna was fractionated on neutral and alkaline csc1 gradients and the heavy strands of satellite i annealed in situ to denatured polytene chromosomes from squash preparations of larval salivary glands. nuclease s1 from aspergillus oryzae was used to digest the unpaired ssdna, resulting in a distinct labeling of the alpha-heterochromatin in th ...19761253642
nuclease s1 cleavage and the primary structure of mitochondrial dna.the single-strand-specific nuclease s1 from aspergillus oryzae rapidly converts superhelical mitochondrial dna (african green monkey cells, vero atcc; ccl 81) into nicked circular dna. these nicked mitochondrial dna molecules contain two nicks, one in each strand. the phosphodiester backbones are cleaved during this reaction at or near sites that are alkali-labile. in a second slow reaction the circular mitochondrial dna is converted into a linear duplex dna. permutation tests indicate that this ...19761261542
purification and properties of the nuclease inhibitor of aspergillus oryzae and kinetics of its interaction with crystalline nuclease o.a nuclease inhibitor found in the mycelia of aspergillus oryzae has been purified 158,000-fold by ammonium sulfate precipitation, chromatography on sephadex g-75, deae-sephadex a-50 and bio-gel p-60 columns, preparative disc electrophoresis on acrylamide gel, and electrofocusing in ampholite. the purified inhibitor is nearly homogeneous as judged by disc electrophoresis. it shows a typical ultraviolet absorption curve for protein, and the inhibitory activity is inactivated by chymotrypsin. the i ...19761262346
sepcific fragmentation of dna heteroduplex molecules of two bacteriophage lambda mutants with endonuclease si from aspergillus oryzae.heteroduplex dna molecules of two bacteriophage mutants (lambda b2 and lambda i434ct68) were obtained by the method of molecular hybridization. these heteroduplexes possessed two types of loops formed as a result of: a) deletion in one of the dna strands; and b) substitution of a dna fragment for nonhomological one. the digestion of heteroduplexes with single-stranded specific nuclease si from aspergillus oryzae produced two fragments at 37 degrees c and three ones at 55 degrees c. the separatio ...19761272803
aflatoxin production by a variant of aspergillus oryzae (nrrl strain 1988) on cowpeas (vigna sinensis).aflatoxin b1, b2, g1, and g2 are produced when a variant of aspergillus oryzae (nrrl strain 1988) is grown on cowpeas or rice. the present study indicates that a strain of aspergillus oryzae approved for use in food processing is variable and the resulting variant, unlike the parent strain, has a propensity to produce aflatoxin.19761273594
the structure of kinetoplast dna. 2. characterization of a novel component of high complexity present in the kinetoplast dna network of crithidia luciliae.1. degradation of highly purified kinetoplast dna (kdna) networks with restriction endonucleases yields "extra" bands in agarose gels that are absent from digests of mini-circles. each of the five endonucleases tested, i.e. alui, hapii, ecori, hsu and hindii + iii, yields a unique set of "extra" bands. the "extra" bands consist of linear dna; they are not mini-circle oligomers and their added molecular weight, calculated from mobility in gels, are around 2 x 10(7). double digests with two restri ...19761278151
[isolation and properties of the acid carboxypeptidase of aspergillus oryzae].caboxypeptidase with ph optimum 4--5 for peptide substrates is isolated from "oryzine", the enzyme mixture produced by aspergillus oryzae, by means of successive salt fractionation, sephadex g-75 gel filtration, chromatography on amberlite irc-50, hydroxylapatite, deae-cellulose and polyacrylamide gel electrophoresis. its molecular weight, as determined by means of polyacrylamide gel electrophoresis in the presence of sodium dolecylsulphate, was found to be 37 000. the enzyme has a broad substra ...1976942547
fungus-fermented soybeans benefit the life cycle of japanese quail (coturnix coturnix japonica).feeding quail chicks diets containing soybeans fermented with two cultures of aspergilli (a. oryzae n.r.r.l. 451 and a. oryzae n.r.r.l. 506) resulted in significantly superior weight gains (p less than 0.05) through a 4-week growth period and confirmed previous observations made with identical cultures in broiler studies. subsequent hen-day egg production and egg size were changed little by diets containing fermented soybeans. the numerical increases in fertility and hatchability were not signif ...1976945574
aspergillus oryzae a patient with only meningitis, a septate hypha was seen in a langhans giant cell, and the rarely pathogenic aspergillus oryzae was cultured from the cerebrospinal fluid. serologic results confirmed the diagnosis. the patient responded to therapy with amphotericin b and flucytosine.1976946540
[effect of the storage conditions on the viability of fungi]. 1976950926
visualization of leucineaminopeptidase activity after acrylamide gel electrophoresis. 1976962113
attachment of thioglycosides to proteins: enhancement of liver membrane binding.thioglycosides of d-galactose, d-glucose, n-acetyl-d-glucosamine, and d-mannose were covalently attached to aspergillus oryzae alpha-amylase, hen's eggs lysozyme, and bovine serum albumin by amidination, diazo coupling, and amide formation. the binding of the newly formed glycoproteins (neoglycoproteins) to rabbit liver membranes was measured, using asialoorosomucoid as a reference. attachment of d-galactosides by any of the three methods enhanced binding by several orders of magnitude. coupling ...1976963013
quantitative analysis of the action of taka-amylase a on maltotriose.the action of taka-amylase a from asp. oryzae was studied quantitatively by the product analysis method using unlabeled maltotriose and maltotriose labeled at the reducing end as substrates. it was found that the ratio of the unlabeled products, maltose (g2) and glucose (g1) exceeded unity, and that the ratio of the labeled products, g2/g1 was strongly dependent on the initial substrate concentrations. the results can only be explained by a transglycosylation or condensation mechanism or both. a ...1976977557
[production of immobilized alpha-amylase and its properties].active immobilized alpha-amylase was obtained when applying ae-cellulose chromatography and glutaric dialdehyde. the time of the enzyme interaction with the carrier, amount of glutaric dialdehyde necessary for binding, optimal enzyme: carrier ratio as well as the methods for desiccation of the immobilized amylase preparations were specified. conditions are selected for alpha-amylase stabilization with the presence of glutaric aldehyde. immobilized amylase as compared to free enzyme is shown to b ...1976982618
fermentative production of limit dextrinase by aspergillus oryzae. 1976992794
aspergillus oryzae (nrrl strain 1988): a clarification. 1976996551
subsite mapping of enzymes. application of the depolymerase computer model to two the preceding paper (allen and thoma, 1976) we developed a depolymerase computer model, which uses a minimization routine to establish a subsite map for a depolymerase. in the present paper we show how the model is applied to experimental data for two alpha-amylases. michaelis parameters and bond-cleavage frequencies for substrates of chain lengths up to twelve glucosyl units have been reported for bacillus amyloliquefaciens, and a subsite map has been proposed for this enzyme [thoma et al. ( ...1976999630
conformation of mammalian 5.8 s ribosomal rna: s1 nuclease as a probe. 19761001452
effect of brinase (protease i from aspergillus oryzae) on the elimination of fibrin from the lungs and pulmonary damage in rats with induced intravascular coagulation and inhibited fibrinolysis. 19761006629
structure of cerebroside in aspergillus oryzae.structural studies on cerebroside isolated from aspergillus oryzae were carried out using mainly gas-liquid chromatography-mass spectrometry. the major component fatty acids were 2-hydroxystearic and 2-hydroxy-trans-octadecenoic acid; branched 17?-methyl nonadecasphingadienine isomers were the predominant long-chain bases. the component sugar was only glucose. the structure of the major cerebrosides was assumed to be n-2'-hydroxystearoyl-1-o-glucosyl-17?-methyl sphingadienine and n-2'-hydroxyoct ...19761009131
proteinase inhibitors from cereal grains. 19761012027
[hydrolysis of insoluble bone collagen by crystalline alpha-amylase from aspergillus oryzae].the paper deals with hydrolysis of bone collagen thrice recrystallized alpha-amylase. the enzyme action was estimated by the amount of released glycopeptides, free carbohydrates, alpha-amine groups and total nitrogen in the soluble part of the hydrolyzate. the protease admixture in the alpha-amylase preparation was found by means of the aspergillus oryzae protease. the data obtained testify to the fact that hydrolysis of collagen under the effect of crystalline alpha-amylase occurs only due to t ...19761021920
studies on lipids of natto. 1976815306
[degradation of the human igg molecule by a fungal fraction with leucineaminopeptidase activity. preliminary results]. 1976819165
[peptidases of preparations of proteolytic enzymes from several species of bacteria, molds and actinomycetes].the activity of carboxy- and aminopeptidases of enzymic preparations obtained from bacteria bacillus subtilis 1 m, 17, bacillus mesentericus 100/26, 100/56, 16; pseudomonas aeruginosa 1/7a, 1/4; bacillus thermophilicus s. sp. nov b-22, moulds aspergillus oryzae 1-746, aspergillus flavus 716 and actinomycete streptomyces griseus 32-13 was studied. in the bacterial preparations the proteolytic activity was 1.5-2.5 times lower than in the fungal and actinomycete preparations. the carboxypeptidase a ...1976825850
characterization of beta-galactosidase from a special strain of aspergillus oryzae.beta-galactosidase ec was isolated from a partially purified preparation obtained from cultured cells of a special strain of aspergillus oryzae, rt 102 (ferm-p1680). the enzyme preparation gave a single protein band on polyacrylamide gel electrophoresis and was free from alpha-galactosidase, alpha- and beta-mannosidase, alpha- and beta-n-acetylhexosaminidase, and protease activities. the beta-galactosidase was capable of acting on aryl beta-galactosides, lactose, and lactosides. it also ...197614118
mechanism of amylase action on glucoside starch bonds.functional groups of glucoamylase and alpha-amylase from asp. awamori, alpha-amylase from asp. oryzae and alpha- and beta-amylases from barley malt are identified. kinetic curves of the activity dependency on ph, values of ionization heats and photooxidative inactivation draw to the conclusion that carboxyl-imidazole system enters into the active site of the enzymes. a hypothetic mechanism of hydrolysis of alpha-1,4-glucoside bond in starch molecule by alpha- and beta-amylases and of alpha-1,4- ...197614726
purification and characterization of the two molecular forms of aspergillus oryzae acid protease.the isolation and partial characterization of the acid proteases a1 and a2 (ec3.4.23.6) from aspergillus oryzae grown on solid bran culture are described. the purified preparations were essentially homogeneous by several criteria including sedimentation analysis and polyacrylamide gel electrophoresis. the physiochemical properties of the proteases a1 and a2 were as follows (in the order: a1, a2): molecular weight: 63 000 & 32 000; sedimentation coefficient s20, w: 3.93 and 3.16 s; diffusion cons ...19768138
enzymatic determination of nonrandom incorporation of 5-bromodeoxyuridine in rat dna.secondary cultures of normal rat embryo cells were synchronized by a double thymidine block and pulsed with 10(-7) m 5-[3h]bromodeoxyuridine (brdurd) or 10(-7) m[3h]thymidine during an entire s phase (7.5 h). to examine the pattern of [3h]thymidine, dna was immediately extracted and purified at the completion of the s phase, cscl density gradient centrifugation revealed that substitution for thymine by bromouracil was less than 7%. single-strand specific nucleases obtained from aspergillus oryza ...1976133713
evidence for the presence of membrane-bound forms of acid protease in aspergillus oryzae. 197713792
use of amylum derivatives for isolation of amylolytic a leading article literature is reviewed concerning isolation of amylolytic enzymes by adsorption on differently modified starches, resp. on other adsorbents. deae amylum, deahp amylum and deae-sephadex a 25 were found to be most suitable adsorbents. the other adsorbents examined did not reach claimed parameters.197715219
isolation of amylolytic system of aspergillus oryzae by sorption on deahp amylum.conditions of effective sorption of amylolytic enzyme from a solution or from fermentative liquid on deahp amylum were studied. isolating action is in a direct dependence on the relation between activity and amount of deahp amylum, the curve of this dependence was illustrated. the enzyme can be released by elution or adsorbate can be used in a pulverised from. in the conclusion of the work laboratory isolation technique is described.197715220
extracellular acid protease of aspergillus oryzae grown on liquid media: multiple forms due to association with heterogeneous polysaccharides.the acid protease (ec that is produced extracellularly when aspergillus oryzae is grown on liquid media has been isolated and characterized. the enzyme was purified by precipitation with tannic acid, chromatography on duolite a-2, and gel filtration on sephadex g-100. the last step yielded four active components, with varying molecular weights ranging from 42,000 to 60,000. two of them, designated e1 and e1a, with molecular weights of 60,000 and 55,000, respectively, were heterogeneous ...197715987
gluconic acid forming enzymes in aspergillus niger (author's transl).at least three gluconic acid forming enzymes were identified in cell-free extracts of aspergillus niger: glucose oxidase (ec, a glucose dehydrogenase (ec, and an enzyme or a mixture of enzymes catalyzing the cleavage of 6-phosphogluconate into gluconate and inorganic phosphate. 2,6-dichlorphenolindophenol was one of the hydrogen acceptors in vitro of the glucose dehydrogenase. some properties of this enzyme (km values, ph-dependence, substrate and hydrogen acceptor specificit ...197716416
properties of chymotrypsin proteinase from aspergillus oryzae.chymotrypsin-type proteinase is detected in the proteolytic system of asp. oryzae. the action of it and chymotrypsin is shown to depend on formaldehyde. hydrolysis of substrates, p-nitrophenyl acetate (p-npa) and n-benzoyl-tyrosine methyl ether (btme), by both preparations is almost the same. the obtained activity ph-optimum for the studied proteinase esterolytic activity is located in the alkaline zone as well as for crystalline chymotrypsin (substrate p-npa). it concerns ph of both enzymes sta ...197718830
rapid induction of alpha-amylase by nongrowing mycelia of aspergillus oryzae.a rapid induction system for synthesis of alpha-amylase by the funga aspergillus oryzae m-13 was established. the mycelia were prepared from 20-h cultures grown on a peptone-glycerol medium and starved for 5 h; maltose was the optimum inducer tested. during h 1 of induction, formation of both intra- and extracellular alpha-amylases occurred at an almost identical rate (70 to 80 microgram/g of cells-h) without a detectable lag period. after a 1-h induction period, a remarkable increase in the ext ...197718989
preparation and properties of a new dnase from aspergillus oryzae.a dnase present in commercial preparations of aspergillus oryzae alpha-amylase was purified 1550-fold in 25% yield by acetone precipitation and by chromatography on diethylaminoethyl- and carboxymethylcellulose. the enzyme was isolated free of contaminating rnases and dnases. the molecular weight of the enzyme determined by gel filtration on sephadex g-100 was 48 000, while a molecular weight of 58 000 was determined for the single band observed upon polyacrylamide gel electrophoresis in sodium ...197719042
isolation and properties of leucine aminopeptidase from aspergillus oryzae.homogenious leucine aminopeptidase is purified from "oryzine"--mixture of enzymes produced by surface culture of asperigillus oryzae using treatment with activated characoal, followed by deae-cellulose and hydroxylapatite chromatographies, biogel p-100 gel-filtration and polyacrylamide-gel electrophoresis. the enzyme has ph optimum 9.0 and the molecular weight 37500 as estimated by gil-filtration through sephadex g-100 (superfine) and sds-polyacrylamide gel electrophoresis. leucine aminopeptidas ...197719097
optimal conditions of alpha-amylase production by aspergillus oryzae in liquid media.the alpha-amylase secretion in a mineral culture medium containing starch and glucose follow the lysis of mycelium. this lysis seems to result from the hydrolysing action of dextranase and levulanase on cell wall. cell lysis and amylase secretion are greatly enhanced by ph elevation of culture medium (optimal ph 8,8). in such conditions of production the amylase is not stable but can be stabilized by addition of starch. a method is described using ph and starch content modifications, which allow ...197723718
characterization of dna used to assay sera for anti-dna antibodies; determination of the specificities of anti-dna antibodies in sle and non-sle rheumatic disease states.commercial 14c-labeled kb cell dna, widely used to assay sera for anti-dna antibodies, was chromatographed on benzoylated-naphthoylated-deae-cellulose (bndc) and on hydroxyapatite (hap). on bndc, only 25% of the 14c label eluted with 1 m nac1 (kb fraction i) characteristic of ds-dna. fifty-five percent of the label eluted with 50% formamide-1 m nac1 (kb fraction ii) characteristic of ss or denatured dna. on hap, however, none of the 14c label eluted with 0.2 m phosphate buffer as anticipated for ...1977300090
isolation of amylolytic system of aspergillus oryzae on deae amylum.studying isolation technique with deae amylum the method was found to be suitable for isolation of alpha-amylase. after determination of optimal conditions sorption curves were illustrated, from which the amount of deae amylum needed for 100% enzyme sorption from solutions of various activity and origin can be calculated. preparation obtained shows high temperature stability and can be used in food industry and agriculture or after its elution in pharmacy. at the end of the paper laboratory isol ...1977846561
aspergillus oryzae (nrrl strain 1988) 1977867035
chemical properties of the polysaccharides associated with acid protease of aspergillus oryzae grown on solid bran media. 1977881411
s1 nuclease from aspergillus oryzae for the detection of dna damage and repair in the gamma-irradiated intracerebral rat gliosarcoma 9l. 1977905506
degradation of nucleic acids in cell lysates by s1 nuclease in the presence of 9 m urea and sodium dodecylsulfate.single-strand-specific nuclease s1 from aspergillus oryzae is shown to degrade dna and rna in lysates of hela cells in the presence of 9 m urea and sodium dodecylsulfate. free dodecylsulfate inhibits s1 nuclease. however, if the detergent is complexed with proteins prior to the addition of the enzyme, s1 nuclease can degrade nucleic acids at dodecylsulfate concentrations which would inhibit the enzyme completely if no other proteins were present. in lysates prepared from hela cells by treatment ...1977908332
specific hydrolysis of rabbit globin messenger rna by s1 nuclease.s1 nuclease isolated from aspergillus oryzae has been used to investigate the secondary structure of rabbit globin messenger rna (mrna). the enzyme, which is specific for single stranded nucleotides, digests globin mrna to a limited extent, with 65-75% of the mrna nucleotides resistant to digestion under mild conditions. this limited digestion is not due to enzyme inactivation, but rather to the normal activity of the single-strand nuclease. the reaction was studied as a function of temperature, ...1977909777
isolation and amino-terminal sequence analysis of a new pancreatic trypsinogen of the african lungfish protopterus aethiopicus.the purification and characterization of three pancreatic trypsinogens a1, a2, and a3, from the african lungfish, protopterus aethiopicus, is reported. these zymogens are activated by trypsin, by enterokinase, by an acid protease from aspergillus oryzae, and by autoactivation. the three trypsinogens contain the same amino-terminal amino acid sequence, beginning with the activation peptide leu-pro-leu-glu-asp-asp-lys-. like the activation peptide of the previously characterized trypsinogen b [ree ...1977911766
natural plant enzyme inhibitors. v. a trypsin/chymotrypsin inhibitor from alocasia macrorhiza tuber.a trypsin/chymotrypsin inhibitor was isolated from the tubers of alocasia macrorhiza by extraction at ph 7.6, heat treatment at 80 degrees c, ammonium sulphate precipitation and successive column chromatography on cm-cellulose, deae-sephadex a-50 and sephadex g-100. the inhibitor was pure by cellulose acetate electrophoresis. the molecular weight was approximately 32 000 as determined by gel filtration on sephadex g-100. the inhibitor acted on bovine trypsin, human trypsin and bovine chymotrypsi ...1977911861
kinetic studies of the phenol sulphate-phenol sulphotransferase of aspergillus oryzae.arylsulphatase ii of aspergillus oryzae exhibits both hydrolytic and sulphotransferase activities. the kinetic data suggest the formation of an intermediate covalent enzyme-sulphate complex with transfer of sulphate from donor to acceptor proceeding via a ping pong mechanism. the unusual kinetic behaviour when 2-hydroxy-5-nitrophenyl sulphate is the substrate is also consistent with this mechanism.1977588253
a rapid direct assay for the determination of the separate activities of the three arylsulphatases of aspergillus oryzae.1. the three arylsulphatases of aspergillus oryzae exhibit pronounced kinetic differences and substrate specificities. arylsulphatase i hydrolyses all substrates tested, whereas arylsulphatase iii will not hydrolyse tyrosine o-sulphate or phenolphthalein disulphate. arylsulphatase ii does not hydrolyse p-nitrophenyl sulphate or phenolphthalein disulphate at appreciable rates in the absence of added phenolic compounds. phenols such as tyramine increase the rate of hydrolysis of these substances b ...1977597235
differential repression of arylsulphatase synthesis in aspergillus oryzae.1. the activities of the three arylsulphatases (arylsulphate sulphohydrolase, ec of aspergillus oryzae produced under a variety of repressing and non-repressing conditions were determined. 2. these enzymes exhibit different sensitivities to repression by inorganic sulphate. 3. arylsulphatase i, but not arylsulphatases ii and iii, exhibits a transient de-repression in the early growth phase in sulphate media. 4. when the fungus is cultured in repressing media and subsequently transferred ...1977597236
release of peptide-bound sialic acid from landschütz ascites-tumour cells by proteinase 1 of aspergillus oryzae [proceedings]. 1977598591
mycotoxins of aspergillus oryzae strains for use in the food industry as starters and enzyme producing molds. 1977613924
studies on the respiratory system of aspergillus oryzae. v. some properties of the respiratory system of mitochondria from mycelia grown in the presence of chloramphenicol.presence of chloramphenicol in the growth medium for mycelia of aspergillus oryzae was without effect on the oxidative activity, respiratory control, or p/o ratio of isolated mitochondria. the mitochondria oxidized krebs cycle intermediates even in the presence of cyanide at the concentration markedly inhibiting the normal mitochondrial oxidation. however, the p/o ratio during the mitochondrial oxidation decreased by about 1.0 on addition of cyanide. the c-type cytochromes, shown to occur in lar ...1977199770
inside 45s ribonucleic acid. 1977202281
mechanism of conjugation and recombination in bacteria. xvii. further evidence for single-stranded regions in recipient dna during conjugation in escherichia coli k-12.the secondary structure of recipient dna mated with hfr strain was investigated by cscl density gradient fractionation. after 45 min of hfrh64 x 3h-f-ab1157 mating one-fourth of the radioactive recipient dna was recovered as a single-strand but only after shearing of cell lysates prior to centrifugation. this heavier than native dna fraction of radioactive material (obtained after the first centrifugation) was degraded by single-strand specific nuclease s from aspergillus oryzae. these findings ...197767752
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