TitleAbstractYear(sorted ascending)
stereoselective behavior of the chiral herbicides diclofop-methyl and diclofop during the soy sauce brewing process.chiral pesticides are now receiving more and more attention in the food-making process. this experiment studied the enantioselective behavior of diclofop-methyl (dm) and its main metabolite, diclofop (dc), during the soy sauce brewing process. two kinds of commonly used strains, aspergillus oryzae and saccharomyces rouxii, were investigated. however, they showed a different degradation ability to the enantiomers of dm and dc. it was observed that (-)-(s)-dm was degraded much faster than (+)-(r)- ...201626501920
heterologous expression and characterization of a thermostable exo-β-d-glucosaminidase from aspergillus exo-β-d-glucosaminidase (aorcsxa) from aspergillus oryzae fl402 was heterologously expressed and purified. the deduced amino acid sequence indicated that aorcsxa belonged to glycoside hydrolase family 2. aorcsxa digested colloid chitosan into glucosamine but not into chitosan oligosaccharides, demonstrating exo-β-d-glucosaminidase (csxa) activity. aorcsxa exhibited optimal activity at ph 5.5 and 50°c; however, the enzyme expressed in pichia pastoris (ppaorcsxa) showed much stronger thermostab ...201626597529
an unusual chimeric diterpene synthase from emericella variecolor and its functional conversion into a sesterterpene synthase by domain swapping.di- and sesterterpene synthases produce c20 and c25 isoprenoid scaffolds from geranylgeranyl pyrophosphate (ggpp) and geranylfarnesyl pyrophosphate (gfpp), respectively. by genome mining of the fungus emericella variecolor, we identified a multitasking chimeric terpene synthase, evvs, which has terpene cyclase (tc) and prenyltransferase (pt) domains. heterologous gene expression in aspergillus oryzae led to the isolation of variediene (1), a novel tricyclic diterpene hydrocarbon. intriguingly, i ...201626546087
reconstitution of biosynthetic machinery of fungal polyketides: unexpected oxidations of biosynthetic intermediates by expression host.reconstitution of whole biosynthetic genes in aspergillus oryzae has successfully applied for total biosynthesis of various fungal natural products. heterologous production of fungal metabolites sometimes suffers unexpected side reactions by host enzymes. in the studies on fungal polyketides solanapyrone and cytochalasin, unexpected oxidations of terminal olefin of biosynthetic intermediates were found to give one and four by-products by host enzymes of the transformants harboring biosynthetic g ...201626539980
a long natural-antisense rna is accumulated in the conidia of aspergillus oryzae.analysis of expressed sequence tag libraries from various culture conditions revealed the existence of conidia-specific transcripts assembled to putative conidiation-specific reductase gene (csra) in aspergillus oryzae. however, the all transcripts were transcribed with opposite direction to the gene csra. the sequence analysis of the transcript revealed that the rna overlapped mrna of csra with 3'-end, and did not code protein longer than 60 amino acid residues. we designated the transcript con ...201626539735
a biosensor based on gold nanoparticles stabilized in poly(allylamine hydrochloride) and decorated with laccase for determination of nanoparticles stabilized in poly(allylamine hydrochloride) (aunp-pah) were used as a support for the immobilization of the enzyme laccase obtained from genetically-modified microorganisms (aspergillus oryzae) and successfully applied in the development of a new biosensor for the determination of dopamine by square-wave voltammetry. the electrochemical characterization of the biosensor was performed by cyclic voltammetry and electrochemical impedance spectroscopy and indicated that the nanom ...201626566574
aspergillus oryzae fermented germinated soybean extract alleviates perimenopausal symptoms in ovariectomised rats.soybeans have been widely used to alleviate climacteric symptoms. in this study, we investigated the oestrogenic activities of isoflavones extracted from aspergillus oryzae-challenged germinated soybeans (ao-gs). eight-week-old virgin sprague-dawley female rats were ovariectomised (ovx). the rats were orally administered 0.1 mg kg(-1) 17α-ethinyl oestradiol or three different doses of ao-gs (0.5, 1.0 2.0 g kg(-1) day(-1)) in distilled water for 6 weeks, while control rats were administered vehic ...201625777819
microbial bioconversion and processing methods enhance the phenolic acid and flavonoids and the radical scavenging capacity of smilax china l. has been reported that smilax china l. leaf (scl) provided various biological functions owing to polyphenols. the objective of the current study was to assess the enhancing effect of processing methods and microbial conversions on phenolic acid and flavonoid content and radical scavenging capacity of scl for potential applications of diverse food products.201625754764
production of the natural iron chelator deferriferrichrysin from aspergillus oryzae and evaluation as a novel food-grade antioxidant.deferriferrichrysin (dfcy) is a siderophore found in foods fermented by aspergillus oryzae and is a promising candidate for an antioxidant food additive because of its high binding constant toward iron. however, the dfcy concentration is typically low in foods and cultures.201626399367
purification and enzymatic characterization of a novel β-1,6-glucosidase from aspergillus this study, among the 10 genes that encode putative β-glucosidases in the glycoside hydrolase family 3 (gh3) with a signal peptide in the aspergillus oryzae genome, we found a novel gene (ao090038000425) encoding β-1,6-glucosidase with a substrate specificity for gentiobiose. the transformant harboring ao090038000425, which we named bglh, was overexpressed under the control of the improved glaa gene promoter to form a small clear zone around the colony in a plate assay using 4-methylumbellife ...201626320404
thermostabilization of aspergillus oryzae β-d-galactosidase.the thermal stability of the aspergillus oryzae β-d-galactosidase (β-gal) was evaluated at 60 °c. the stability of β-gal was dependent on the ph, buffer, and additives. the β-gal exhibited its highest thermal stability in a 0.02 m phosphate buffer ph 6. among all the tested additives, galactose, a competitive inhibitor of β-gal, was the upmost thermal stabilizer. a 0.15 m galactose solution caused β-gal to retain a whole of 98.65% of its initial activity after incubation at 60 °c for 2 h. the se ...201626011544
phylogenetic and structural analysis of polyketide synthases in aspergilli.polyketide synthases (pkss) of aspergillus species are multidomain and multifunctional megaenzymes that play an important role in the synthesis of diverse polyketide compounds. putative pks protein sequences from aspergillus species representing medically, agriculturally, and industrially important aspergillus species were chosen and screened for in silico studies. six candidate aspergillus species, aspergillus fumigatus af293, aspergillus flavus nrrl3357, aspergillus niger cbs 513.88, aspergill ...201627199544
genome sequence of aspergillus luchuensis nbrc 4314.awamori is a traditional distilled beverage made from steamed thai-indica rice in okinawa, japan. for brewing the liquor, two microbes, local kuro (black) koji mold aspergillus luchuensis and awamori yeast saccharomyces cerevisiae are involved. in contrast, that yeasts are used for ethanol fermentation throughout the world, a characteristic of japanese fermentation industries is the use of aspergillus molds as a source of enzymes for the maceration and saccharification of raw materials. here we ...201627651094
korean ginseng berry fermented by mycotoxin non-producing aspergillus niger and aspergillus oryzae: ginsenoside analyses and anti-proliferative transform ginsenosides, korean ginseng berry (kgb) was fermented by mycotoxin non-producing aspergillus niger and aspergillus oryzae. changes of ginsenoside profile and anti-proliferative activities were observed. results showed that a. niger tended to efficiently transform protopanaxadiol (ppd) type ginsenosides such as rb1, rb2, rd to compound k while a. oryzae tended to efficiently transform protopanaxatriol (ppt) type ginsenoside re to rh1 via rg1. butanol extracts of fermented kgb showed ...201627582326
a set of isogenic auxotrophic strains for constructing multiple gene deletion mutants and parasexual crossings in aspergillus construct a set of isogenic auxotrophic strains in aspergillus niger suited for creating multiple gene deletion mutants and executing parasexual crossings, we have combined mutations in genes involved in colour pigmentation (fwna and olva) with well-selectable auxotrophic markers (pyrg, nicb, argb, and adea). all markers, except for the pyrg marker, were introduced by targeted deletion, omitting uv mutagenesis of the strains. aspergillus oryzae orthologous genes of the argb, nicb, and adea ma ...201627251039
nutrient assessment of olive leaf residues processed by solid-state fermentation as an innovative feedstuff additive.olive leaf residue feedstuff additives were prepared by solid-state fermentation (ssf), and its feeding effects on broiler chickens were examined.201626991541
in vitro cytotoxicity and antimicrobial activity of talaromyces flavus sp5 inhabited in the marine sediment of southern coast of india.marine sediment samples were collected from the coastal areas of southern india, particularly in kanyakumari district. twenty-eight different fungal strains were isolated. the screening of fungi from marine sediment was done to isolate a potent fungus that can produce bioactive compounds for biomedical applications. only three strains viz trichoderma gamsii sp4, talaromyces flavus sp5 and aspergillus oryzae sp6 were screened for further studies. the intracellular bioactive compounds were extract ...201628262118
evaluation of pyrolysis oil as carbon source for fungal fermentation.pyrolysis oil, a complex mixture of several organic compounds, produced during flash pyrolysis of organic lignocellulosic material was evaluated for its suitability as alternative carbon source for fungal growth and fermentation processes. therefore several fungi from all phyla were screened for their tolerance toward pyrolysis oil. additionally aspergillus oryzae and rhizopus delemar, both established organic acid producers, were chosen as model organisms to investigate the suitability of pyrol ...201628066378
application of lectin array technology for biobetter characterization: its correlation with fcγriii binding and adcc.lectin microarray technology was applied to compare the glycosylation pattern of the monoclonal antibody mb311 expressed in sp2.0 cells to an antibody-dependent cellular cytotoxic effector function (adcc)-optimized variant (mb314). mb314 was generated by a plant expression system that uses genetically modified moss protoplasts (physcomitrella patens) to generate a de-fucosylated version of mb311. in contrast to mb311, no or very low interactions of mb314 with lectins aspergillus oryzae l-fucose ...201628029136
rice koji extract enhances lipid metabolism through proliferator-activated receptor alpha (pparα) activation in mouse liver.koji is made from grains fermented with aspergillus oryzae and is essential for the production of many traditional japanese foods. many previous studies have shown that koji contributes to the improvement of dyslipidemia. however, little is known regarding the underlying mechanism of this effect. furthermore, the compound contributing to the activation of lipid metabolism is unknown. we demonstrated that rice koji extract (rke) induces the mrna expression of peroxisome proliferator-activated rec ...201627934292
erratum to: japanese traditional dietary fungus koji aspergillus oryzae functions as a prebiotic for blautia coccoides through glycosylceramide: japanese dietary fungus koji is a new prebiotic.[this corrects the article doi: 10.1186/s40064-016-2950-6.].201627822443
comparison of the effects of dietary single and multi-probiotics on growth, non-specific immune responses and disease resistance in starry flounder, platichthys 8-week feeding trial was conducted to evaluate the effects of dietary probiotics on growth performance and non-specific immune responses in starry flounder, platichthys stellatus. fish averaging 46.5 ± 0.65 g (mean ± sd) were fed one of the six experimental diets; one control (cont), and five other diets were prepared by supplementing single-probiotics 1 (bacillus subtilis; sp1, 2 × 10(9) cfu kg(-1) diet), single-probiotics 2 (bacillus licheniformis; sp2, 2 × 10(9) cfu kg(-1) diet), multi-pro ...201627818339
aflatoxin b1 detoxification by aspergillus oryzae from meju, a traditional korean fermented soybean starter.aflatoxins are classified as group 1 (carcinogenic to humans) by the international agency for research on cancer (iarc). in this study, a total of 134 fungal strains were isolated from 65 meju samples, and two fungal isolates were selected as potential aflatoxin b₁ (afb₁)-biodetoxification fungi. these fungi were identified as aspergillus oryzae mao103 and a. oryzae mao104 by sequencing the beta-tubulin gene. the two a. oryzae strains were able to degrade more than 90% of afb1 (initial concentra ...201627817189
functional characterization of cyp52g3 from aspergillus oryzae and its application for bioconversion and synthesis of hydroxyl flavanone and steroids.aspergillus oryzae is a fungus widely used in traditional japanese fermentation industries. cytochrome p450 (cyp) proteins are ubiquitously distributed in nature and display a broad range of enzymatic activities. a novel cyp52 (cyp52g3) gene was found in a. oryzae. in this study, we report the functional characterization of cyp52g3. the recombinant protein was expressed heterologously in escherichia coli, and its membrane fraction isolated. cyp52g3 showed activities for 7-ethoxycoumarin and α-na ...201627028566
valorization of by-products from palm oil mills for the production of generic fermentation media for microbial oil synthesis.this study demonstrates the production of a generic nutrient-rich feedstock using by-product streams from palm oil production that could be used as a substitute for commercial fermentation supplements. solid-state fermentations of palm kernel cake (pkc) and palm-pressed fiber (ppf) were conducted in tray bioreactors and a rotating drum bioreactor by the fungal strain aspergillus oryzae for the production of crude enzymes. the production of protease was optimized (319.3 u/g) at an initial moistur ...201627787766
a glycoprotein α-amylase inhibitor from withania somnifera differentially inhibits various α-amylases and affects the growth and development of tribolium castaneum.identification and characterisation of plant defensive molecules enrich our resources to design crop protection strategies. in particular, plant-derived proteinaceous inhibitor(s) of insect digestive enzymes appear to be a safe, sustainable and attractive option.201627770482
mitochondria-localized phospholipase a2, aoplaa, in aspergillus oryzae displays phosphatidylethanolamine-specific activity and is involved in the maintenance of mitochondrial phospholipid mammals, cytosolic phospholipases a2 (cpla2s) play important physiological roles by releasing arachidonic acid, a precursor for bioactive lipid mediators, from the biological membranes. in contrast, fungal cpla2-like proteins are much less characterized and their roles have remained elusive. aoplaa is a cpla2-like protein in the filamentous fungus aspergillus oryzae which, unlike mammalian cpla2, localizes to mitochondria. in this study, we investigated the biochemical and physiological funct ...201627634187
subcellular localization of acyl-coa binding protein in aspergillus oryzae is regulated by autophagy eukaryotic cells, acyl-coa binding protein (acbp) is important for cellular activities, such as in lipid metabolism. in the industrially important fungus aspergillus oryzae, the acbp, known as aoacbp, has been biochemically characterized, but its physiological function is not known. in the present study, although we could not find any phenotype of aoacbp disruptants in the normal growth conditions, we examined the subcellular localization of aoacbp to understand its physiological function. us ...201627725156
degradation of 3-phenoxybenzoic acid by a filamentous fungus aspergillus oryzae m-4 strain with self-protection transformation.a novel filamentous fungus m-4 strain was isolated from soy sauce koji and identified as aspergillus oryzae (collection number: cgmcc 11645) on the basis of morphological characteristics and internal transcribed spacer sequence. m-4 could degrade 80.62 % of 3-phenoxybenzoic acid (3-pba; 100 mg l(-1)) within 5 days. 3-pba degradation occurred in accordance with first-order kinetics. the degradation metabolites of 3-pba were identified through high-performance liquid chromatography-mass spectromet ...201627678114
genome mining and motif truncation of glycoside hydrolase family 5 endo-β-1,4-mannanase encoded by aspergillus oryzae rib40 for potential konjac flour hydrolysis or feed additive.two novel glycosyl hydrolase family 5 (gh5) β-mannanases (aoman5a and aoman5b) were identified from aspergillus oryzae rib40 by genome mining. the aoman5a contains a predicted family 1 carbohydrate binding module (cbm-1), located at its n-terminal. the aoman5a, aoman5b and truncated mutant aoman5aδcl (truncating the n-terminal cbm and linker of aoman5a) were expressed retaining the n-terminus of the native protein in pichia pastoris gs115 by ppic9k(m). the specific enzyme activity of the purifie ...201627702490
secretory expression, purification, characterization, and application of an aspergillus oryzae prolyl aminopeptidase in bacillus subtilis.cdna coding a prolyl aminopeptidase (pap) was cloned from aspergillus oryzae and over expressed in bacillus subtilis with a 6×his tag in n-terminus. the recombinant prolyl aminopeptidase was secreted to extracellular by adding 2 mm cacl2 and 5% d-sorbitol in tb medium; the enzyme activity in fermented supernatant increased from 7.2 to 41.5 u ml(-1). it has been purified 4.3-fold through ni-chelating affinity chromatography with a recovery of 47.3%. the purified enzyme is stable below 50 °c and w ...201627889870
simultaneous hydrolysis and co-fermentation of whey lactose with wheat for ethanol production.whey permeate was used as a co-substrate to replace part of the wheat for ethanol production by saccharomyces cerevisiae. the simultaneous saccharification and fermentation was achieved with β-galactosidase added at the onset of the fermentation to promote whey lactose hydrolysis. aspergillus oryzae and kluyveromyces lactis β-galactosidases were two enzymes selected and used in the co-fermentation of wheat and whey permeate for the comparison of their effectiveness on lactose hydrolysis. the pos ...201627693727
co-metabolic degradation of β-cypermethrin and 3-phenoxybenzoic acid by co-culture of bacillus licheniformis b-1 and aspergillus oryzae m-4.the degradation efficiency of organic contaminants and their associated metabolites by co-culture of microbes is mainly limited by toxic intermediates from co-metabolic degradation. in this study, we investigated the degradation of β-cypermethrin (β-cy) and 3-phenoxybenzoic acid (3-pba) by co-culture of bacillus licheniformis b-1 and aspergillus oryzae m-4, as well as the influences of β-cy and 3-pba metabolites on their degradation and the growth of strains b-1 and m-4. our results indicated th ...201627898684
structural and catalytic properties of s1 nuclease from aspergillus oryzae responsible for substrate recognition, cleavage, non-specificity, and inhibition.the single-strand-specific s1 nuclease from aspergillus oryzae is an archetypal enzyme of the s1-p1 family of nucleases with a widespread use for biochemical analyses of nucleic acids. we present the first x-ray structure of this nuclease along with a thorough analysis of the reaction and inhibition mechanisms and of its properties responsible for identification and binding of ligands. seven structures of s1 nuclease, six of which are complexes with products and inhibitors, and characterization ...201628036383
the construction and use of versatile binary vectors carrying pyrg auxotrophic marker and fluorescent reporter genes for agrobacterium-mediated transformation of aspergillus oryzae.aspergillus oryzae is a safe mold widely used in food industry. it is also considered as a microbial cell factory for production of recombinant proteins and enzymes. currently, genetic manipulation of filamentous fungi is achieved via agrobacterium tumefaciens-mediated transformation methods usually employing antibiotic resistance markers. these methods are hardly usable for a. oryzae due to its strong resistance to the common antifungal compounds used for fungal transformation. in this study, w ...201627804102
deletion of afpab1 causes increased sensitivity to oxidative stress and hypovirulence in aspergillus fumigatus.aspergillus fumigatus afpab1 is the ortholog of the aspergillus oryzae cytoplasmic messenger ribonucleoprotein granules aopab1 that function to depress the initiation of translation during stress. a. fumigatus can regulate its cellular physiology in response to environmental stresses, but there has been no research on pab1 in a. fumigatus. the associated gene afpab1 was replaced with a hygromycin-selectable marker to generate the strain δafpab1. phenotypic analysis showed that the δafpab1 grew m ...201627801871
transformation of litchi pericarp-derived condensed tannin with aspergillus awamori.condensed tannin is a ubiquitous polyphenol in plants that possesses substantial antioxidant capacity. in this study, we have investigated the polyphenol extraction recovery and 2,2-diphenyl-1-picrylhydrazyl (dpph) scavenging activity of the extracted polyphenol after litchi pericarp is treated with aspergillus awamori, aspergillus sojae or aspergillus oryzae. we have further explored the activity of a. awamori in the formation of condensed tannin. the treatment of a. awamori appeared to produce ...201627420043
biohydrogen production in the suspended and attached microbial growth systems from waste pastry hydrolysate.waste pastry was hydrolyzed by glucoamylase and protease which were obtained from solid state fermentation of aspergillus awamori and aspergillus oryzae to produce waste pastry hydrolysate. then, the effects of hydraulic retention times (hrts) (4-12h) on hydrogen production rate (hpr) in the suspended microbial growth system (continuous stirred tank reactor, cstr) and attached microbial growth system (continuous mixed immobilized sludge reactor, cmisr) from waste pastry hydrolysate were investig ...201627416509
valorization of bakery waste for biocolorant and enzyme production by monascus purpureus.a concept of utilizing bakery waste as a nutrient source for the fermentative production of bio-colorant by monasucus purpureus has been developed. the proposed ideas provide an innovative approach to establish a system or method to reduce the bakery waste problem encountered by hong kong or other countries. bakery waste collected from bakery store was used in submerged and solid-state fermentation of monascus purpureus to produce bio-colorant, which could potentially be applied in food and text ...201627208753
continuous biohydrogen production from waste bread by anaerobic this study, continuous biohydrogen production from waste bread by anaerobic sludge was performed. the waste bread was first hydrolyzed by the crude enzymes which were generated by aspergillus awamori and aspergillus oryzae via solid-state fermentation. it was observed that 49.78g/l glucose and 284.12mg/l free amino nitrogen could be produced with waste bread mass ratio of 15% (w/v). the waste bread hydrolysate was then used for biohydrogen production by anaerobic sludge in a continuous stirre ...201627065225
novel inexpensive fungi proteases: production by solid state fermentation and characterization.a comparative study was carried out for proteases production using agroindustrial residues as substrate for solid state fermentation (ssf) of several fungal strains. high protease production was observed for most of the microorganisms studied, as well as very different biochemical characteristics, including activities at specific temperatures and a wide range of ph values. the enzymes produced were very different regarding optimum ph and they showed stability at 50 °c. aspergillus oryzae showed ...201626769513
invasive forms of candida and aspergillus in sputum samples of pulmonary tuberculosis patients attending the tuberculosis reference laboratory in ghaemshahr, northern iran: an analysis of samples collected during the past 10years.tuberculosis (tb) continues to be a major public health problem worldwide. this is especially true in northern iran, which has high tb prevalence. the chronic nature of this disease is further exacerbated if it is accompanied by fungal infection, which usually remains undiagnosed and thus untreated. thus, mycotic infections add fatal dimensions to pulmonary tb. our objective was to determine the prevalence of invasive forms of fungal elements in sputum samples collected from patients with pulmon ...201628043539
fungal elicitors enhance ginsenosides biosynthesis, expression of functional genes as well as signal molecules accumulation in adventitious roots of panax ginseng c. a. this work, we selected three fungi strains (aspergillus niger, aspergillus flavus and aspergillus oryzae) as elicitors prepared from mycelium or fermentation broth to improve ginsenosides production in adventitious roots culture. the results showed that ginsenosides production (29.90±4.67mgg(-1)) was significantly enhanced upon elicitation with 200mgl(-1)a. niger elicitor prepared from mycelium, which was 3.52-fold of untreated group. hplc-esi-ms(n) analysis was performed, showing that ginsen ...201627746309
expression of ustr and the golgi protease kexb are required for ustiloxin b biosynthesis in aspergillus oryzae.ustiloxin b, originally isolated from the fungus ustilaginoidea virens, is a known inhibitor of microtubule assembly. ustiloxin b is also produced by aspergillus flavus and is synthesized through the ribosomal peptide synthesis pathway. in a. flavus, the gene cluster associated with ustiloxin b production contains 15 genes including those encoding a fungal c6-type transcription factor and ustiloxin b precursor. although the koji mold aspergillus oryzae, which is genetically close to a. flavus, h ...201626842395
comparative transcriptome analysis revealing dormant conidia and germination associated genes in aspergillus species: an essential role for atfa in conidial dormancy.fungal conidia are usually dormant unless the extracellular conditions are right for germination. despite the importance of dormancy, little is known about the molecular mechanism underlying entry to, maintenance of, and exit from dormancy. to gain comprehensive and inter-species insights, transcriptome analyses were conducted across aspergillus fumigatus, aspergillus niger, and aspergillus oryzae.201627185182
three extracellular dipeptidyl peptidases found in aspergillus oryzae show varying substrate specificities.three extracellular dipeptidyl peptidase genes, dppb, dppe, and dppf, were unveiled by sequence analysis of the aspergillus oryzae genome. we investigated their differential enzymatic profiles, in order to gain an understanding of the diversity of these genes. the three dipeptidyl peptidases were expressed using aspergillus nidulans as the host. each recombinant enzyme was purified and subsequently characterized. the enzymes displayed similar optimum ph values, but optimum temperatures, ph stabi ...201626846741
an overproduction of astellolides induced by genetic disruption of chromatin-remodeling factors in aspergillus oryzae.the filamentous fungus aspergillus oryzae is an important industrial mold. recent genomic analysis indicated that a. oryzae has a large number of biosynthetic genes for secondary metabolites (sms), but many of the sms they produce have not been identified. for better understanding of sms production by a. oryzae, we screened a gene-disruption library of transcription factors including chromatin-remodeling factors and found two gene disruptions that show similarly altered sm production profiles. o ...201626126743
increased enzyme production under liquid culture conditions in the industrial fungus aspergillus oryzae by disruption of the genes encoding cell wall α-1,3-glucan synthase.under liquid culture conditions, the hyphae of filamentous fungi aggregate to form pellets, which reduces cell density and fermentation productivity. previously, we found that loss of α-1,3-glucan in the cell wall of the fungus aspergillus nidulans increased hyphal dispersion. therefore, here we constructed a mutant of the industrial fungus a. oryzae in which the three genes encoding α-1,3-glucan synthase were disrupted (tripleδ). although the hyphae of the tripleδ mutant were not fully disperse ...201627442340
involvement of an srf-mads protein mcma in regulation of extracellular enzyme production and asexual/sexual development in aspergillus nidulans.srf-mads proteins are transcription factors conserved among eukaryotes that regulate a variety of cellular functions; however, their physiological roles are still not well understood in filamentous fungi. effects of a mutation in mcma gene that encodes the sole srf-mads protein in the fungus aspergillus nidulans were examined by rna sequencing. sequencing data revealed that expression levels of cellulase genes were significantly decreased by the mutation as reported previously. however, expressi ...201626967516
cytotoxicity and colloidal behavior of polystyrene latex nanoparticles toward filamentous fungi in isotonic solutions.the effects of surface physicochemical properties of functionalized polystyrene latex (psl) nanoparticles (nps) and model filamentous fungi aspergillus oryzae and aspergillus nidulans cultivated in different environment (aqueous and atmospheric environment) on the colloidal behavior and cytotoxicity were investigated in different isotonic solutions (154 mm nacl and 292 mm sucrose). when the liquid cultivated fungal cells were exposed to positively charged psl nps in 154 mm nacl solution, the nps ...201626855210
self-excising cre/mutant lox marker recycling system for multiple gene integrations and consecutive gene deletions in aspergillus this study, we developed a self-excising cre/loxp-mediated marker recycling system with mutated lox sequences to introduce a number of biosynthetic genes into aspergillus oryzae. to construct the self-excising marker cassette, both the selectable marker, the aspergillus nidulans adea gene, and the cre recombinase gene (cre), conditionally expressed by the xylanase-encoding gene promoter, were designed to be located between the mutant lox sequences, lox66 and lox71. however, construction of th ...201728011085
analysis of the ionic interaction between the hydrophobin roda and two cutinases of aspergillus nidulans obtained via an aspergillus oryzae expression system.hydrophobins are amphipathic secretory proteins with eight conserved cysteine residues and are ubiquitous among filamentous fungi. in the fungus aspergillus oryzae, the hydrophobin rola and the polyesterase cutl1 are co-expressed when the sole available carbon source is the biodegradable polyester polybutylene succinate-co-adipate (pbsa). rola promotes the degradation of pbsa by attaching to the particle surface, changing its structure and interacting with cutl1 to concentrate cutl1 on the pbsa ...201727917435
matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for rapid identification of fungal rhinosinusitis pathogens.filamentous fungi are among the most important pathogens, causing fungal rhinosinusitis (frs). current laboratory diagnosis of frs pathogens mainly relies on phenotypic identification by culture and microscopic examination, which is time consuming and expertise dependent. although matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) ms has been employed to identify various fungi, its efficacy in the identification of frs fungi is less clear.201728100314
a novel zn2-cys6 transcription factor atrr plays a key role in an azole resistance mechanism of aspergillus fumigatus by co-regulating cyp51a and cdr1b expressions.successful treatment of aspergillosis caused by aspergillus fumigatus is threatened by an increasing incidence of drug resistance. this situation is further complicated by the finding that strains resistant to azoles, the major antifungal drugs for aspergillosis, have been widely disseminated across the globe. to elucidate mechanisms underlying azole resistance, we identified a novel transcription factor that is required for normal azole resistance in aspergillus fungi including a. fumigatus, as ...201728052140
optimisation of nutritional requirements for dopamine synthesis by calcium alginate-entrapped mutant strain of aspergillus oryzae ems-6.the optimisation of nutritional requirements for dopamine (da) synthesis by calcium alginate-entrapped mutant variant of aspergillus oryzae ems-6 using submerged fermentation technique was investigated. a total of 13 strains were isolated from soil. isolate i-2 was selected as a better producer of da and improved by exposing with ethyl methylsulphonate (ems). ems-6 was selected as it exhibited 43 μg/ml da activity. the mutant variable was further treated with low levels of l-cysteine hcl to make ...201727686520
simultaneous conversion of free fatty acids and triglycerides to biodiesel by immobilized aspergillus oryzae expressing fusarium heterosporum lipase.the presence of high levels of free fatty acids (ffa) in oil is a barrier to one-step biodiesel production. undesirable soaps are formed during conventional chemical methods, and enzyme deactivation occurs when enzymatic methods are used. this work investigates an efficient technique to simultaneously convert a mixture of free fatty acids and triglycerides (tag). a partial soybean hydrolysate containing 73.04% free fatty acids and 24.81% triglycerides was used as a substrate for the enzymatic pr ...201727860394
enhanced production of fructosyltransferase in aspergillus oryzae by genome breed aspergillus oryzae strains with high fructosyltransferase (ftase) activity using intraspecific protoplast fusion via genome-shuffling.201727853895
aoatg26, a putative sterol glucosyltransferase, is required for autophagic degradation of peroxisomes, mitochondria, and nuclei in the filamentous fungus aspergillus oryzae.autophagy is a conserved process in eukaryotic cells for degradation of cellular proteins and organelles. in filamentous fungi, autophagic degradation of organelles such as peroxisomes, mitochondria, and nuclei occurs in basal cells after the prolonged culture, but its mechanism is not well understood. here, we functionally analyzed the filamentous fungus aspergillus oryzae aoatg26, an ortholog of the sterol glucosyltransferase ppatg26 involved in pexophagy in the yeast pichia pastoris. deletion ...201727696999
detailed analysis of targeted gene mutations caused by the platinum-fungal talens in aspergillus oryzae rib40 strain and a ligd disruptant.transcription activator-like effector nucleases (talens), which can generate dna double-strand breaks at specific sites in the desired genome locus, have been used in many organisms as a tool for genome editing. in aspergilli, including aspergillus oryzae, however, the use of talens has not been validated. in this study, we performed genome editing of a. oryzae wild-type strain via error of nonhomologous end-joining (nhej) repair by transient expression of high-efficiency platinum-fungal talens ...201727780671
mutational analysis of kex2 recognition sites and a disulfide bond in tannase from aspergillus oryzae.aspergillus oryzae tannase (aotana), which contains two kex2 recognition sites at positions arg311 and arg316, consists of two subunits that are generated by the cleavage of tannase gene product by the kex2 protease. based on the crystal structure of feruloyl esterase from aspergillus oryzae (aofaeb), which has been classified as a member of the fungal tannase family, the catalytic triad residues of aotana are predicted to be ser195, asp455, and his501, with the serine and histidine residues bro ...201727919681
cellular responses to the expression of unstable secretory proteins in the filamentous fungus aspergillus oryzae.filamentous fungi are often used as cell factories for recombinant protein production because of their ability to secrete large quantities of hydrolytic enzymes. however, even using strong transcriptional promoters, yields of nonfungal proteins are generally much lower than those of fungal proteins. recent analyses revealed that expression of certain nonfungal secretory proteins induced the unfolded protein response (upr), suggesting that they are recognized as proteins with folding defects in f ...201728064367
deletion of admb gene encoding a fungal adam affects cell wall construction in aspergillus oryzae.mammals possess a unique signaling system based on the proteolytic mechanism of a disintegrin and metalloproteinases (adams) on the cell surface. we found two genes encoding adams in aspergillus oryzae and named them adma and admb. we produced adma and admb deletion strains to elucidate their biological function and clarify whether fungal adams play a similar role as in mammals. the ∆adma∆admb and ∆admb strains were sensitive to cell wall-perturbing agents, congo red, and calcofluor white. moreo ...201728058997
learning from oligosaccharide soaks of crystals of an aa13 lytic polysaccharide monooxygenase: crystal packing, ligand binding and active-site disorder.lytic polysaccharide monooxygenases (lpmos) are a class of copper-dependent enzymes discovered within the last ten years. they oxidatively cleave polysaccharides (chitin, lignocellulose, hemicellulose and starch-derived), presumably making recalcitrant substrates accessible to glycoside hydrolases. recently, the first crystal structure of an lpmo-substrate complex was reported, giving insights into the interaction of lpmos with β-linked substrates (frandsen et al., 2016). the lpmos acting on α-l ...201728045386
glucoamylase: a current allergen in the baking industry.over a 10 year period a decline in the rate of sensitizations to α-amylase (aspergillus oryzae) was observed in bakers investigated for allergic obstructive airway disease. at the same time, glucoamylase (aspergillus niger) was identified as the currently the most relevant allergen in sensitizations to enzymes in the baking industry. the aim of the present study was to investigate whether, over a period of 10 years and in the case of new-onset disease, there had been any change in sensitization ...201726120537
quantitative evaluation of haze formation of koji and progression of internal haze by drying of koji during koji making.the construction of an experimental system that can mimic koji making in the manufacturing setting of a sake brewery is initially required for the quantitative evaluation of mycelia grown on/in koji pellets (haze formation). koji making with rice was investigated with a solid-state fermentation (ssf) system using a non-airflow box (nab), which produced uniform conditions in the culture substrate with high reproducibility and allowed for the control of favorable conditions in the substrate during ...201728373030
cell wall α-1,3-glucan prevents α-amylase adsorption onto fungal cell in submerged culture of aspergillus oryzae.we have previously reported that α-amylase (taka-amylase a, taa) activity disappears in the later stage of submerged aspergillus oryzae culture as a result of taa adsorption onto the cell wall. chitin, one of the major components of the cell wall, was identified as a potential factor that facilitates taa adsorption. however, taa adsorption only occurred in the later stage of cultivation, although chitin was assumed to be sufficiently abundant in the cell wall regardless of the submerged culture ...201728356219
carbon and nitrogen depletion-induced nucleophagy and selective autophagic sequestration of a whole nucleus in multinucleate cells of the filamentous fungus aspergillus oryzae.autophagy is a conserved cellular degradation process in eukaryotes, in which cytoplasmic components and organelles are digested in vacuoles/lysosomes. recently, autophagic degradation of nuclear materials, termed "nucleophagy", has been reported. in the multinucleate filamentous fungus aspergillus oryzae, a whole nucleus is degraded by nucleophagy after prolonged culture. while developing an h2b-egfp processing assay for the evaluation of nucleophagy in a. oryzae, we found that nucleophagy is e ...201728331162
ovicidal effect of chitinase and protease enzymes produced by soil fungi on the camel tick hyalomma dromedarii eggs (acari:ixodidae).the aim of this study was to evaluate the effect of chitinase and protease enzymes produced by environmentally safe soil fungi; aspergillus sp. nrc 4/5h; mucor sp. nrc 5; trichoderma sp. nrc 4/56; aspergillus sojae; mucor ranosisinus; aspergillus oryzae on embryo development of the camel tick hyalomma dromedarii eggs. the experiment was carried out on the 7 day aged eggs. concentrations of the chitinase and protease crude enzymes [1:1, 1:2, 1:3, 1:4 and 1:5 (v/v)] were prepared from six stock so ...201728316424
high-efficiency extracellular release of free fatty acids from aspergillus oryzae using non-ionic fatty acids (ffas) are useful for generating biofuel compounds and functional lipids. microbes are increasingly exploited to produce ffas via metabolic engineering. however, in many microorganisms, ffas accumulate in the cytosol, and disrupting cells to extract them is energy intensive. thus, a simple cost-effective extraction technique must be developed to remove this drawback. we found that ffas were released from cells of the filamentous fungus aspergillus oryzae with high efficiency whe ...201728300661
biosorption of copper (ii) from aqueous solutions by sclerotiogenic aspergillus oryzae g15.this study aimed at investigating the biosorption of the copper(ii) from aqueous solutions by sclerotiogenic aspergillus oryzae g15. potentiometric titration analysis results indicated that carboxyl group was mainly responsible for cu(ii) adsorption. sclerotia were superior biosorbent than mycelia, which could be ascribed to the higher amount of carboxyl sites exposed after differentiation. langmuir isotherm model fitted well the absorption process of mycelia and sclerotia with the maximum theor ...201728276318
analysis of microbial diversity in shenqu with different fermentation times by pcr-dgge.shenqu is a fermented product that is widely used in traditional chinese medicine (tcm) to treat indigestion; however, the microbial strains in the fermentation process are still unknown. the aim of this study was to investigate microbial diversity in shenqu using different fermentation time periods. dgge (polymerase chain reaction-denaturing gradient gel electrophoresis) profiles indicated that a strain of pediococcus acidilactici (band 9) is the predominant bacteria during fermentation and tha ...201728256389
functional expression of a novel α-amylase from antarctic psychrotolerant fungus for baking industry and its magnetic immobilization.α-amylase plays a pivotal role in a broad range of industrial processes. to meet increasing demands of biocatalytic tasks, considerable efforts have been made to isolate enzymes produced by extremophiles. however, the relevant data of α-amylases from cold-adapted fungi are still insufficient. in addition, bread quality presents a particular interest due to its high consummation. thus developing amylases to improve textural properties could combine health benefits with good sensory properties. fu ...201728245836
a comparative secretome analysis of industrial aspergillus oryzae and its spontaneous mutant zjgs-lz-21.aspergillus oryzae koji plays a crucial role in fermented food products due to the hydrolytic activities of secreted enzymes. in the present study, we performed a comparative secretome analysis of the industrial strain of aspergillus oryzae 3.042 and its spontaneous mutantzjgs-lz-21. one hundred and fifty two (152) differential protein spots were excised (p<0.05), and 25 proteins were identified. of the identified proteins, 91.3% belonged to hydrolytic enzymes acting on carbohydrates or proteins ...201728237882
immobilization of laccase from aspergillus oryzae on graphene nanosheets.laccase enzymes of aspergillus oryzae were immobilized on graphene nanosheets by physical adsorption and covalent bonding. morphological features of the graphene sheets were characterized via microscopy techniques. the immobilization by adsorption was carried out through contact between graphene and solution of laccase enzyme dissolved in deionized water. the adsorption process followed a freundlich model, showing no tendency to saturation within the range of values used. the process of immobili ...201728237573
aos28d, a proline-xaa carboxypeptidase secreted by aspergillus oryzae.prolyl peptidases of the merops s28 family are of particular interest because they are key enzymes in the digestion of proline-rich peptides. a blast analysis of the aspergillus oryzae genome revealed sequences coding for four proteases of the s28 family. three of these proteases, aos28a, aos28b, and aos28c, were previously characterized as acidic prolyl endopeptidases. the fourth protease, aos28d, showed high sequence divergence with other s28 proteases and belongs to a phylogenetically distinc ...201728229206
immobilization of aspergillus oryzae β-galactosidase in an agarose matrix functionalized by four different methods and application to the synthesis of lactulose.aspergillus oryzae β-galactosidase was immobilized in monofunctional glyoxyl-agarose and heterofunctional supports (amino-glyoxyl, carboxy-glyoxyl and chelate-glyoxyl agarose), for obtaining highly active and stable catalysts for lactulose synthesis. specific activities of the amino-glyoxyl agarose, carboxy-glyoxyl agarose and chelate-glyoxyl agarose derivatives were 3676, 430 and 454iu/g biocatalyst with half-life values at 50°c of 247, 100 and 100h respectively. specific activities of 3490, 25 ...201728214445
positive and negative aspects of green coffee consumption - antioxidant activity versus mycotoxins.the quality of coffee depends not only on the contents of healthy compounds but also on its contamination with microorganisms that can produce mycotoxins during development, harvesting, preparation, transport and storage.201728195330
reaction kinetics and galactooligosaccharide product profiles of the β-galactosidases from bacillus circulans, kluyveromyces lactis and aspergillus oryzae.β-galactosidase enzymes are used in the dairy industry to convert lactose into galactooligosaccharides (gos) that are added to infant formula to mimic the molecular sizes and prebiotic functions of human milk oligosaccharides. here we report a detailed analysis of the clearly different gos profiles of the commercial β-galactosidases from bacillus circulans, kluyveromyces lactis and aspergillus oryzae. also the gos yields of these enzymes differed, varying from 48.3% (b. circulans) to 34.9% (k. l ...201728193420
fed-batch operation for the synthesis of lactulose with β-galactosidase of aspergillus oryzae.fed-batch synthesis of lactulose from lactose and fructose with aspergillus oryzae β-galactosidase was evaluated, obtaining a concentration of 40.4g·l(-1), which is 20% higher than obtained in batch, while the concentration of transgalactosylated oligosaccharides (tos) was reduced by 98%. therefore, selectivity of lactulose synthesis can be significantly higher by operating in fed-batch mode. the enzyme-limiting substrate mass ratio (e/s) is a critical variable in fed-batch operation. higher val ...201728162924
synthesis of lactulose in batch and repeated-batch operation with immobilized β-galactosidase in different agarose functionalized supports.lactulose synthesis was done in repeated-batch mode with aspergillus oryzae β-galactosidase immobilized in glyoxyl-agarose (ga-βg), amino-glyoxyl-agarose (am-ga-βg) and chelate-glyoxyl-agarose (che-ga-βg), at fructose/lactose molar ratios of 4, 12 and 20. highest yields of lactulose in batch were obtained with che-ga-βg (0.21, 0.29 and 0.32g·g(-1)) for 4, 12 and 20 fructose/lactose molar ratios respectively; when operating in 10 repeated batches highest product to biocatalyst mass ratios were ob ...201728160659
effects of nonionic surfactants on pellet formation and the production of β-fructofuranosidases from aspergillus oryzae kb.aspergillus oryzae kb produces two β-fructofuranosidases (f1 and f2). f1 has high transferring activity and produces fructooligosaccharides from sucrose. mycelial growth pellets were altered by the addition of tween 20, 40 and 80 (hlb=16.7, 15.6 and 15.0, respectively) in liquid medium cultures to form small spherical pellets. the particle size of the pellets decreased with the hlb value, which corresponds to an increase in surfactant hydrophobicity. selective f1 production and pellet size were ...201728159248
tolerance and stress response of sclerotiogenic aspergillus oryzae g15 to copper and lead.aspergillus oryzae g15 was cultured on czapek yeast extract agar medium containing different concentrations of copper and lead to investigate the mechanisms sustaining metal tolerance. the effects of heavy metals on biomass, metal accumulation, metallothionein (mt), malondialdehyde (mda), superoxide dismutase (sod), catalase (cat), and peroxidase (pod) were evaluated. cu and pb treatment remarkably delayed sclerotial maturation and inhibited mycelial growth, indicating the toxic effects of the m ...201728132138
identification of a novel penicillium chrysogenum rhamnogalacturonan rhamnohydrolase and the first report of a rhamnogalacturonan rhamnohydrolase gene.rhamnogalacturonan (rg) i is one of the main components of pectins in the plant cell wall. we recently reported two rg i-degrading enzymes, endo-rg and exo-rg lyases, secreted by penicillium chrysogenum 31b. here, our aims were to purify a rg rhamnohydrolase (pcrgrh78a) from the culture filtrate of this strain and to characterize this enzyme. on the basis of the internal amino acid sequences, the encoding gene, pcrgrh78a, was cloned and overexpressed in aspergillus oryzae. the deduced amino acid ...201728110667
biochemical characterization of thermostable β-1,4-mannanase belonging to the glycoside hydrolase family 134 from aspergillus oryzae.a β-1,4-mannanase, termed aoman134a, that belongs to the gh 134 family was identified in the filamentous fungus aspergillus oryzae. recombinant aoman134a was expressed in pichia pastoris, and the purified enzyme produced mannobiose, mannotriose, mannotetraose, and mannopentaose from galactose-free β-mannan, with mannotriose being the predominant reaction product. the catalytic efficiency (k cat/k m ) of aoman134a was 6.8-fold higher toward galactomannan from locust bean gum, than toward galactom ...201728105485
microbial diversity during fermentation of sweet paste, a chinese traditional seasoning, using pcr-denaturing gradient gel electrophoresis.the aim of this study was to elucidate the changes in the microbial community and biochemical properties of a traditional sweet paste during fermentation. pcr-denaturing gradient gel electrophoresis (dgge) analysis showed that aspergillus oryzae was the predominant species in the koji and the majority of the fungi isolated belonged to 2 zygosaccharomyces species in the mash. the bacterial dgge profiles revealed the presence of bacillus subtilis during fermentation, and lactobacillus acidipiscis, ...201728081358
evaluating the effects of microwave-assisted hydrodistillation on antifungal and radical scavenging activities of oliveria decumbens and chaerophyllum macropodum essential this study, radical scavenging and antifungal activities of chaerophyllum macropodum and oliveria decumbens essential oils (eos) extracted with microwave-assisted hydrodistillation (mahd) were evaluated in comparison with the same eos extracted by conventional hydrodistillation (hd). the final eo yields that were obtained using hd (after 150 min) and mahd (after 45 min) were 1.72 and 1.67% for c. macropodum and 8.10 and 7.91% for o. decumbens, respectively. there were no significant differenc ...201728371590
mixed colonies of aspergillus niger and aspergillus oryzae cooperatively degrading wheat both natural and man-made environments, microorganisms live in mixed populations, while in laboratory conditions monocultures are mainly used. microbial interactions are often described as antagonistic, but can also be neutral or cooperative, and are generally associated with a metabolic change of each partner and cause a change in the pattern of produced bioactive molecules. a. niger and a. oryzae are two filamentous fungi widely used in industry to produce various enzymes (e.g. pectinases, ...201728232095
effect of magnesium acetate on the antimold activity of lactobacillus.the antimold activity of lactic acid bacteria (lab) is used in food biopreservation. the aim of this study was to evaluate the effect of magnesium acetate added to de man rogosa sharpe (mrs) medium on the antimold activity of three lab strains ( lactobacillus plantarum , lactobacillus brevis , and lactobacillus fermentum ) against molds contaminating food ( aspergillus oryzae , aspergillus niger , penicillium chrysogenum , fusarium avenaceum , and rhizopus arrhizus ) and their ability to produce ...201728221876
proteolysis in tempeh-type products obtained with rhizopus and aspergillus strains from grass pea (lathyrus sativus) seeds.tempeh is a food product obtained from legumes by means of solid-state fermentation with rhizopus sp. our previous research proved that mixed-culture inoculum may also be successfully applied. the objective of present research was to study the proteolytic activity of r. microsporus var. chinensis and a. oryzae during tempeh-type fermentation of grass pea seeds, and the effect of inoculum composition on the protein level and in vitro protein bioavailability in products.201728068010
harnessing biodiesel-producing microbes: from genetic engineering of lipase to metabolic engineering of fatty acid biosynthetic pathway.microbial production routes, notably whole-cell lipase-mediated biotransformation and fatty-acids-derived biosynthesis, offer new opportunities for synthesizing biodiesel. they compare favorably to immobilized lipase and chemically catalyzed processes. genetically modified whole-cell lipase-mediated in vitro route, together with in vivo and ex vivo microbial biosynthesis routes, constitutes emerging and rapidly developing research areas for effective production of biodiesel. this review presents ...201726526353
modeling and verification of process parameters for the production of tannase by aspergillus oryzae under submerged fermentation using agro-wastes.tannase production by aspergillus oryzae using various agro-wastes as substrates by submerged fermentation was studied in this research. the microbe was isolated from degrading corn kernel obtained from the corn fields at tiruchengode, india. the microbial identification was done using 18s rrna gene analysis. the agro-wastes chosen for the study were pomegranate rind, cassia auriculata flower, black gram husk, and tea dust. the process parameters chosen for optimization study were substrate conc ...201726498327
homology modeling and molecular dynamics study on schwanniomyces occidentalis alpha-amylase.with consumers growing increasingly aware of environmental issues, industries find enzymes as a reasonable alternative over physical conditions and chemical catalysts. amylases are important hydrolase enzymes, which have been widely used in variety of industrial process such as pharmaceutical, food, and fermentation industries. among amylases α-amylase is in maximum demand due to its wide range of applications. the homology modeling study on schwanniomyces occidentalis amylase (amy1, uniprot ide ...201726882245
characterizations of the submerged fermentation of aspergillus oryzae using a fullzone impeller in a stirred tank bioreactor.a fullzone (fz) impeller was used in the first study of the characteristics involved in the fermentation of aspergillus oryzae. both the experimental and simulation results of this study revealed novel findings into the positive relationship between the global-axial mixing patterns of a fz impeller and fermentation efficiency. the mixing results when using the fz impeller compared with a double rushton turbine (drt) impeller indicated that the culture mixed by the fz resulted in a more homogeneo ...201727475924
fermented brown rice extract stimulates bdnf gene transcription in c6 glioma cells: possible connection with ho-1 expression.fermented brown rice with aspergillus oryzae, designated as fbra, is known to be commercially available dietary fiber-rich food, which is appreciated as prebiotics to improve intestinal microflora, and also shown to contain various biologically active substances including polyphenolic compounds. on the other hand, polyphenolic compounds have been suggested to stimulate the expression of brain-derived neurotrophic factor (bdnf) gene in connection with the expression of heme oxidase-1 (ho-1) gene ...201727560964
effects of addition of aspergillus oryzae culture and 2-hydroxyl-4-(methylthio) butanoic acid on milk performance and rumen fermentation of dairy investigate effects of aspergillus oryzae culture (aoc) and 2-hydroxy-4-(methylthio) butanoic acid (hmb) on milk performance and rumen fermentation of dairy cows. sixty-four multiparous chinese holstein cows were randomly allocated into four experimental diets: (i) control diet; (ii) aoc diet: 5 g aoc/day per head; (iii) hmb diet: 25 g hmb/day; and (iv) ah diet: 5 g aoc plus 25 g hmb/day. added hmb tended to increase the yield of milk protein (p = 0.06) and 3.5% fat-corrected milk (p = 0.08) ...201727506446
enzymatic production of 5'-inosinic acid by amp deaminase from a newly isolated aspergillus oryzae.5'-adenylic acid deaminase (amp deaminase), an important enzyme for the food industry, can catalyze the irreversible hydrolysis of adenosine monophosphate (amp) to inosine monophosphate (imp) and ammonia. in this study, a new strain was screened that efficiently produces 3191.6u/g of amp deaminase at 32°c. after purification, the optimal temperature and ph of the amp deaminase were found to be 40°c and 6.0, respectively, but it was partially inhibited by fe(3+), cu(2+), al(3+), and zn(2+). with ...201727596420
comparative thermal inactivation analysis of aspergillus oryzae and thiellavia terrestris cutinase: role of glycosylation.cutinase thermostability is important so that the enzymes can function above the glass transition of what are often rigid polymer substrates. a detailed thermal inactivation analysis was performed for two well-characterized cutinases, aspergillus oryzae cutinase (aoc) and thiellavia terrestris cutinase (ttc). both aoc and ttc are prone to thermal aggregation upon unfolding at high temperature, which was found to be a major reason for irreversible loss of enzyme activity. our study demonstrates t ...201727425027
production and biochemical characterization of an alkaline protease from aspergillus oryzae this study, aspergillus oryzae ch93 was isolated from soil sample and examined using molecular analysis. following culture of a. oryzae ch93 under optimal enzyme production, a 47.5kda extracellular protease was purified using ammonium sulfate precipitation and q-sepharose chromatography. the optimal ph 8 and temperature of 50°c obtained for the isolated protease. sodium dodecyl sulfate (sds), cetyltrimethyl ammonium bromide (ctab), h2o2 decreased activity, while triton x-100 and phenylmethane ...201727293035
purification and characterization of a newly serine protease inhibitor from rhamnus frangula with potential for use as therapeutic drug.protease inhibitors from plants are well known to be potent inhibitors of the growth of bacteria, fungi, and even certain viruses which make them excellent candidates for use as the lead compounds for the development of novel antimicrobial agents for applications in medicine. in this study, rhamnus frangula was selected as a protease inhibitor source. the maximum recovery of the protease inhibitor against trypsin was recorded in the crude extract made in 0.1 m phosphate buffer (ph 7.0) and isola ...201728597160
a new and efficient approach for construction of uridine/uracil auxotrophic mutants in the filamentous fungus aspergillus oryzae using agrobacterium tumefaciens-mediated transformation.aspergillus oryzae is a filamentous fungus widely used in food industry and as a microbial cell factory for recombinant protein production. due to the inherent resistance of a. oryzae to common antifungal compounds, genetic transformation of this mold usually requires auxotrophic mutants. in this study, we show that agrobacterium tumefaciens-mediated transformation (atmt) method is very efficient for deletion of the pyrg gene in different aspergillus oryzae wild-type strains to generate uridine/ ...201728466303
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