TitleAbstractYear(sorted ascending)
functional analysis of aoatg11 in selective autophagy in the filamentous fungus aspergillus oryzae.autophagy is a highly conserved cellular degradation process in eukaryotes and consists of both non-selective and selective types. selective autophagic processes include pexophagy, mitophagy, and the cytoplasm-to-vacuole targeting (cvt) pathway of yeast, in which particular vacuolar proteins, such as aminopeptidase i (ape1), are selectively transported to vacuoles. although selective autophagy has been mainly studied in the yeasts saccharomyces cerevisiae and pichia pastoris, there is evidence f ...201526058532
identification and enzymatic characterization of an endo-1,3-β-glucanase from euglena gracilis.euglena produces paramylon as a storage polysaccharide, and is thought to require β-1,3-glucan degrading enzymes to release and utilize the accumulated carbohydrate. to investigate β-1,3-glucan degradation in euglena, endo-1,3-β-glucanases were partially purified from euglena gracilis by hydrophobic, gel filtration and anion-exchange chromatography. tryptic digests and mass-spectrometric analysis identified three proteins in the purified fraction as a member of glycoside hydrolase family (gh) 17 ...201526028521
preventive effects of fermented brown rice and rice bran against n-nitrosobis (2-oxopropyl) amine-induced pancreatic tumorigenesis in male hamsters.fermented brown rice by aspergillus oryzae (fbra) is known to have the potential to prevent chemical carcinogenesis of the colon, liver, esophagus, urinary bladder, stomach and lungs in rodents. the present study examined the possible chemopreventive effects of fbra on n-nitrosobis(2-oxopropyl)amine (bop)-induced pancreatic tumorigenesis in hamsters. five-week-old male syrian golden hamsters were divided into seven groups. groups 1-5 were subcutaneously injected with bop (10 mg/kg body weight) f ...201526788138
ethanol and protein from ethanol plant by-products using edible fungi neurospora intermedia and aspergillus oryzae.feasible biorefineries for production of second-generation ethanol are difficult to establish due to the process complexity. an alternative is to partially include the process in the first-generation plants. whole stillage, a by-product from dry-mill ethanol processes from grains, is mostly composed of undegraded bran and lignocelluloses can be used as a potential substrate for production of ethanol and feed proteins. ethanol production and the proteins from the stillage were investigated using ...201526682213
fermented brown rice and rice bran with aspergillus oryzae (fbra) prevents inflammation-related carcinogenesis in mice, through inhibition of inflammatory cell infiltration.we have established an inflammation-related carcinogenesis model in mouse, in which regressive qr-32 cells subcutaneously co-implanted with a foreign body-gelatin sponge-convert themselves into lethal tumors due to massive infiltration of inflammatory cells into the sponge. animals were fed with a diet containing 5% or 10% fermented brown rice and rice bran with aspergillus oryzae (fbra). in 5% and 10% fbra diet groups, tumor incidences were lower (35% and 20%, respectively) than in the non-trea ...201526670250
towards effective and stable probiotics.probiotics are live microorganisms, generally either lactobacilli or bifidobacteria, which when administered in adequate amounts confer a health benefit to the host [1]. due to the growing evidence of health benefits associated with their use, probiotics are of increasing interest and represent now a significant growth area in the functional foods industry [2]. however, to be effective, orally administered probiotics should survive preparation of dosage forms and passage through acidic environme ...201526639716
antioxidant activity and anti-inflammatory activity of ethanol extract and fractions of doenjang in lps-stimulated raw 264.7 macrophages.fermentation can increase functional compounds in fermented soybean products, thereby improving antioxidant and/or anti-inflammatory activities. we investigated the changes in the contents of phenolics and isoflavones, antioxidant activity and anti-inflammatory activity of doenjang during fermentation and aging.201526634044
supplementation with aspergillus oryzae-fermented kochujang lowers serum cholesterol in subjects with hyperlipidemia.kochujang, a traditional fermented red pepper paste, is known for its hypocholesterolemic effect; however, these studies used non-commercial preparations of kochujang. in this study, we examined whether commercially-made kochujang in which aspergillus oryzae (also known as koji) was used as a microorganism for fermentation has the same cholesterol-lowering effects.201524961447
telomere-mediated chromosomal truncation in aspergillus oryzae.we truncated the short arm of chromosome 3 to delete the aflatoxin biosynthesis gene homolog cluster using telomeric repeats in aspergillus oryzae. the predicted deletion was confirmed by southern blot analyses. this telomere-mediated chromosomal truncation method enables the development of an artificial chromosome in a. oryzae.201525034635
a combination of acid lactase from aspergillus oryzae and yogurt bacteria improves lactose digestion in lactose maldigesters synergistically: a randomized, controlled, double-blind cross-over trial.lactose digestion can be improved in subjects with impaired or completely absent intestinal lactase activity by administration of lactase preparations and particularly of acid lactase, which is active in the stomach, or by yogurt containing live lactic acid bacteria. it is the question, if lactose digestion can be further enhanced by combining these two approaches.201525042846
expression of key hydrolases for soy sauce fermentation in zygosaccharomyces rouxii.several key hydrolases in soy sauce fermentation such as proteases, peptidases, and glutaminases are supplied by aspergillus sojae or aspergillus oryzae. the genes encoding these hydrolases were successfully expressed in salt-tolerant yeast zygosaccharomyces rouxii. these transformants are expected to supply extra hydrolases during soy sauce fermentation process.201525073685
optimization of the condition for adsorption of gallic acid by aspergillus oryzae mycelia using box-behnken design.fresh biomass of aspergillus oryzae (a. oryzae) cgmcc5992 can effectively remove gallic acid from aqueous solution. to improve the removal rate of gallic acid, this study first identified the important factors affecting the removal rate of gallic acid with univariate analysis, and then used four-factor and three-level box-behnken design (bbd) with the removal rate of gallic acid as response value, to obtain the optimum conditions for the removal of gallic acid as follows: 6.95 h treatment time, ...201525109471
identification of functional cis-elements required for repression of the taka-amylase a gene under secretion stress in aspergillus oryzae.the expression of secreted proteins in filamentous fungi is down-regulated by a transcriptional feedback mechanism under endoplasmic reticulum stress, termed repression under secretion stress (ress). to investigate the ress mechanism, we analyzed the expression of the taka-amylase a gene (amyb) in aspergillus oryzae, which was depressed under secreted protein stress. we conducted a truncation and deletion analysis of the amyb promoter to identify cis-elements required for ress. a nucleotide sequ ...201525280730
fungal hemicellulose-degrading enzymes cause physical property changes concomitant with solubilization of cell wall polysaccharides.physical properties of wheat coleoptile segments decreased after treatment with hemicellulose-degrading enzymes, indicating that hemicellulosic polysaccharides function to control the strength of primary cell walls. changes in the physical properties of plant cell walls, a viscoelastic structure, are thought to be one of the growth-limiting factors for plants and one of the infection-affecting factors for fungi. to study the significance of hemicellulosic polysaccharides that form cross-bridges ...201525301670
c7-prenylation of tryptophanyl and o-prenylation of tyrosyl residues in dipeptides by an aspergillus terreus prenyltransferase.during our search for novel prenyltransferases, a putative gene ateg_04218 from aspergillus terreus raised our attention and was therefore amplified from strain dsm 1958 and expressed in escherichia coli. biochemical investigations with the purified recombinant protein and different aromatic substrates in the presence of dimethylallyl diphosphate revealed the acceptance of all the tested tryptophan-containing cyclic dipeptides. structure elucidation of the main enzyme products by nmr and ms anal ...201525125042
effects of koji-making with mixed strains on physicochemical and sensory properties of chinese-type soy sauce.two kinds of soy sauces were prepared with aspergillus oryzae koji (ssao) and mixed koji (ssaom, a. oryzae mouldstarter:monascus purpureus mouldstarter = 1:2, w/w) respectively. the effects of mixed koji on the essential indices, oxygen radical absorption capacity, color indices, free amino acids and volatile compounds of soy sauce have been studied, followed by a sensory evaluation between ssao and ssaom.201525312834
cloning and expression of a novel prolyl endopeptidase from aspergillus oryzae and its application in beer stabilization.a novel prolyl endopeptidase gene from aspergillus oryzae was cloned and expressed in pichia pastoris. amino acid sequence analysis of the prolyl endopeptidase from aspergillus oryzae (ao-pep) showed that this enzyme belongs to a class serine peptide s28 family. expression, purification and characterization of ao-pep were analyzed. the optimum ph and temperature were ph 5.0 and 40 °c, respectively. the enzyme was activated and stabilized by metal ion ca(2+) and inhibited by zn(2+), mn(2+), al(3+ ...201525547787
modulating endoplasmic reticulum-golgi cargo receptors for improving secretion of carrier-fused heterologous proteins in the filamentous fungus aspergillus oryzae.filamentous fungi are excellent hosts for industrial protein production due to their superior secretory capacity; however, the yield of heterologous eukaryotic proteins is generally lower than that of fungal or endogenous proteins. although activating protein folding machinery in the endoplasmic reticulum (er) improves the yield, the importance of intracellular transport machinery for heterologous protein secretion is poorly understood. here, using aspergillus oryzae as a model filamentous fungu ...201525362068
isolation and expression of enolase gene in fusarium oxysporum f. sp. lycopersici.fusarium oxysporum f. sp. lycopersici is a fungus responsible for the tomato disease known as fusariosis. enolase, which is the enzyme that catalyzes the reaction of 2-phosphoglycerate to phosphoenolpyruvate, is present during glycolysis. enolase genes have been isolated from bacteria and fungi, among other organisms. in this research, a large portion of the enolase, eno, gene sequence was isolated from f. oxysporum and compared with those of other microorganisms, revealing a similarity of 51-69 ...201525349089
characterization of the production of biogenic amines and gamma-aminobutyric acid in the soybean pastes fermented by aspergillus oryzae and lactobacillus brevis.the production of gamma-aminobutyric acid (gaba) using gaba-producing lactic acid bacteria (lab) has been considered to be an attractive strategy. however, some lab may produce biogenic amines (ba), which may be of concern from the safety viewpoint. the aim of the present study was to characterize the production of gaba and ba in the soybean pastes fermented by aspergillus oryzae (a. oryzae) fmb s46471 and gaba-producing lactobacillus brevis (l. brevis) gaba 100. after a ripening period of 90 da ...201525341471
change in enzyme production by gradually drying culture substrate during solid-state fermentation.the influence of drying the culture substrate during solid-state fermentation on enzyme production was investigated using a non-airflow box. the drying caused a significant increase in enzyme production, while the mycelium content decreased slightly. this suggests that changes in the water content in the substrate during culture affect enzyme production in fungi.201525488496
distinct mechanism of activation of two transcription factors, amyr and malr, involved in amylolytic enzyme production in aspergillus oryzae.the production of amylolytic enzymes in aspergillus oryzae is induced in the presence of starch or maltose, and two zn2cys6-type transcription factors, amyr and malr, are involved in this regulation. amyr directly regulates the expression of amylase genes, and malr controls the expression of maltose-utilizing (mal) cluster genes. deletion of malr gene resulted in poor growth on starch medium and reduction in α-amylase production level. to elucidate the activation mechanisms of these two transcri ...201525487891
the ctng gene encodes carbonic anhydrase involved in mycotoxin citrinin biosynthesis from monascus aurantiacus.citrinin, a fungal secondary metabolite of polyketide origin, is moderately nephrotoxic to vertebrates, including humans. citrinin is synthesised by condensation of acetyl-coa and malonyl-coa. six genes involved in the citrinin biosynthesis, including pksct, ctna and ctnb, have been cloned in monascus purpureus. the pksct gene encodes a polyketide synthase; ctna is a regulatory factor; and ctnb encodes an oxidoreductase. when the three genes were respectively disrupted, the disruption strains dr ...201525482072
effect of the dose of exogenous fibrolytic enzyme preparations on preingestive fiber hydrolysis, ruminal fermentation, and in vitro digestibility of bermudagrass haylage.our objectives were to evaluate the effects of the dose rates of 5 trichoderma reesei and aspergillus oryzae exogenous fibrolytic enzymes (efe; 1a, 2a, 11c, 13d, and 15d) on in vitro digestibility, fermentation characteristics, and preingestive hydrolysis of bermudagrass haylage and to identify the optimal dose of each efe for subsequent in vitro and in vivo studies. in experiment 1, efe were diluted in citrate-phosphate buffer (ph 6) and applied in quadruplicate in each of 2 runs at 0× (control ...201525468699
molecular characterization of intergeneric hybrid between aspergillus oryzae and trichoderma harzianum by protoplast fusion.protoplast fusion between aspergillus oryzae and trichoderma harzianum and application of fusant in degradation of shellfish waste.201525444252
purification and characterisation of α-amylase produced by mutant strain of aspergillus oryzae ems-18.α-amylase produced by a mutant strain of aspergillus oryzae ems-18 has been purified to homogeneity as judged by sodium dodecyle sulphate polyacrylamide gel electrophoresis (sds-page). the enzyme was purified by using 70% ammonium sulphate precipitation followed by anion exchange chromatography on deae-sephadex column and gel filtration on sephadex g-100. an enzyme purification factor of 9.5-fold was achieved with a final specific activity of 1987.7 u/mg protein and overall yield of 23.8%. the m ...201525424747
multi-response optimisation of the extraction solvent system for phenolics and antioxidant activities from fermented soy flour using a simplex-centroid design.a simplex-centroid design comprising three solvents (water, ethanol and methanol) was used to optimise the extraction mixture for phenolics and antioxidant activities from defatted soy flour fermented with monascus purpureus or aspergillus oryzae. total phenolics were more efficiently extracted using only water for both samples. the highest antioxidant activities by the dpph and abts methods were obtained using extraction mixtures containing at least 75 wt% water. specific water:ethanol:methanol ...201626616938
novel 4-methyl-2-oxopentanoate reductase involved in synthesis of the japanese sake flavor, ethyl leucate.ethyl-2-hydroxy-4-methylpentanoate (ethyl leucate) contributes to a fruity flavor in japanese sake. the mold aspergillus oryzae synthesizes leucate from leucine and then the yeast saccharomyces cerevisiae produces ethyl leucate from leucate during sake fermentation. here, we investigated the enzyme involved in leucate synthesis by a. oryzae. the a. oryzae gene/cdna encoding the enzyme involved in leucate synthesis was identified and expressed in e. coli and a. oryzae host cells. the purified rec ...201626615399
evaluation of kojic acid production in a repeated-batch pcs biofilm this study, kojic acid, a secondary metabolite as an industrially important compound, was produced by aspergillus oryzae (a. oryzae), which was immobilized in plastic composite support (pcs) bioreactor. nitrogen deficient medium was applied to increase the production of ka in pcs-immobilized bioreactor. the efficiency of immobilized culture for kojic acid (ka) production and the effect of morphology of a. oryzae on ka production were evaluated. after three cycles of cultivation, 83.47 g/l of ...201626657710
recombinant expression, purification, and characterization of an acyl-coa binding protein from aspergillus characterize biochemically the lipid metabolism-regulating acyl-coa binding protein (acbp) from the industrially-important fungus aspergillus oryzae.201626677124
enzymatically hydrolysed, acetylated and dually modified corn starch: physico-chemical, rheological and nutritional properties and effects on cake quality.corn starch was treated by enzymatic hydrolysis with aspergillus oryzae s2 α-amylase, acetylation with vinyl acetate, and dual modification. the dual modified starch displayed a higher substitution degree than the acetylated starch and lower reducing sugar content than the hydrolysed starch. the results revealed that the cooling viscosity and amylose content of those products decrease (p < 0.05). an increase in moisture, water, and oil absorption capacity was observed for the acetylated starch a ...201626787967
fermented brown rice extract causes apoptotic death of human acute lymphoblastic leukemia cells via death receptor pathway.mixture of brown rice and rice bran fermented with aspergillus oryzae, designated as fbra, has been reported to reveal anti-carcinogenic and anti-inflammatory effects in rodents. then, to test its potential anti-cancer activity, the aqueous extract was prepared from fbra powder, and the effect of this extract on human acute lymphoblastic leukemia jurkat cells was directly examined. the exposure to fbra extract reduced the cell viability in a concentration- and time-dependent manner. the reductio ...201626769704
identification of the gene encoding isoprimeverose-producing oligoxyloglucan hydrolase in aspergillus oryzae.aspergillus oryzae produces a unique β-glucosidase, isoprimeverose-producing oligoxyloglucan hydrolase (ipase), that recognizes and releases isoprimeverose (α-d-xylopyranose-(1 → 6)-d-glucopyranose) units from the non-reducing ends of oligoxyloglucans. a gene encoding a. oryzae ipase, termed ipea, was identified and expressed in pichia pastoris. with the exception of cellobiose, ipea hydrolyzes a variety of oligoxyloglucans and is a member of the glycoside hydrolase family 3. xylopyranosyl branc ...201626755723
fucosylation is associated with the malignant transformation of intraductal papillary mucinous neoplasms: a lectin microarray-based study.intraductal papillary mucinous neoplasm (ipmn) is an intraductal mucin-producing pancreatic neoplasm with the potential for malignant transformation. changes in glycans expressed on the cell surface and glycotransferases play important roles in malignant transformation. we conducted this study to analyze glycan alterations in ipmns by using a lectin microarray and to identify the factors associated with altered glycans and their relationships with malignant transformation.201626754572
illuminating the binding interactions of galactonoamidines during the inhibition of β-galactosidase (e. coli).several galactonoamidines were previously identified as very potent competitive inhibitors that exhibit stabilizing hydrophobic interactions of the aglycon in the active site of β-galactosidase (aspergillus oryzae). to elucidate the contributions of the glycon to the overall inhibition ability of the compounds, three glyconoamidine derivatives with alteration in the glycon at c-2 and c-4 were synthesized and evaluated herein. all amidines are competitive inhibitors of β-galactosidase (escherichi ...201626740154
production of 3-oxo-2-(2'-pentenyl)-cyclopentane-1-octanoic acid in the fungus aspergillus oryzae: a step towards heterologous production of pyrethrins in fungi.pyrethrins are natural insecticides, which accumulate to high concentrations in pyrethrum (chrysanthemum cinerariaefolium) flowers. synthetic pyrethroids are more stable, more efficacious and cheaper, but contemporary requirements for safe and environmentally friendly pesticides encourage a return to the use of natural pyrethrins, and this would be favoured by development of an efficient route to their production by microbial fermentation. the biosynthesis of pyrethrins involves ester linkage be ...201626718544
improving 3'-hydroxygenistein production in recombinant pichia pastoris using periodic hydrogen peroxide-shocking strategy.3'-hydroxygenistein can be obtained from the biotransformation of genistein by the engineered pichia pastoris x-33 strain, which harbors a fusion gene composed of cyp57b3 from aspergillus oryzae and a cytochrome p450 oxidoreductase gene (scpr) from saccharomyces cerevisiae. p. pastoris x-33 mutants with higher 3'-hydroxygenistein production were selected using a periodic hydrogen peroxide-shocking strategy. one mutant (p2-d14-5) produced 23.0 mg/l of 3'-hydroxygenistein, representing 1.87-fold m ...201626699751
development of a genome editing technique using the crispr/cas9 system in the industrial filamentous fungus aspergillus develop a genome editing method using the crispr/cas9 system in aspergillus oryzae, the industrial filamentous fungus used in japanese traditional fermentation and for the production of enzymes and heterologous proteins.201626687199
metabolic engineering of long chain-polyunsaturated fatty acid biosynthetic pathway in oleaginous fungus for dihomo-gamma linolenic acid production.microbial lipids are promising alternative sources of long chain-polyunsaturated fatty acids (lc-pufas) for food, feed, nutraceutical and pharmaceutical sectors. dihomo-γ-linolenic acid (c20:3δ(8,11,14); dgla) is an important lc-pufas with anti-inflammatory and anti-proliferative effects. to generate a dgla-producing strain, fatty acid reconstitution in aspergillus oryzae was performed by metabolic engineering through co-expression of codon-optimized pythium δ(6)-desaturase and δ(6)-elongase, wh ...201626686314
chitosan crosslinked with genipin as support matrix for application in food process: support characterization and β-d-galactosidase order to develop safer processes for the food industry, we prepared a chitosan support with the naturally occurring crosslinking reagent, genipin, for enzyme. as application model, it was tested for the immobilization of β-d-galactosidase from aspergillus oryzae. chitosan particles were obtained by precipitation followed by adsorption of the enzyme and crosslinking with genipin. the particles were characterized by fourier transform infrared (ftir) spectroscopy and thermogravimetric analysis ( ...201626686119
preventive effects of fermented brown rice and rice bran against prostate carcinogenesis in trap rats.fermented brown rice and rice bran with aspergillus oryzae (fbra) is considered to have the potential to prevent chemically-induced carcinogenesis in multiple organs of rodents. in the present study, we evaluated the possible chemopreventive effects of fbra against prostate tumorigenesis. six-week-old male rats of the transgenic rat for adenocarcinoma of prostate (trap) strain were fed diets containing 5% or 10% fbra for 15 weeks. animals were sacrificed at 21 weeks of age, and the ventral and l ...201627409632
comparative genomic analysis identified a mutation related to enhanced heterologous protein production in the filamentous fungus aspergillus oryzae.genomic mapping of mutations using next-generation sequencing technologies has facilitated the identification of genes contributing to fundamental biological processes, including human diseases. however, few studies have used this approach to identify mutations contributing to heterologous protein production in industrial strains of filamentous fungi, such as aspergillus oryzae. in a screening of a. oryzae strains that hyper-produce human lysozyme (hly), we previously isolated an aut1 mutant tha ...201627405439
identification and heterologous expression of the topopyrone nonaketide synthase gene from phoma sp.non-reducing iterative type i polyketide synthase genes, pnk1 and pnk2, were cloned from the fungus phoma sp. baua2861, which produces the topoisomerase i inhibitors, topopyrones a to d. heterologous expression of these polyketide synthase genes under the α-amylase promoter in aspergillus oryzae was carried out to identify their functions. the pnk2 transformant produced topopyrones c, d, and haematommone. therefore, the pnk2 gene was found to encode for the topopyrone nonaketide synthase.201627373651
structural characterization, catalytic, kinetic and thermodynamic properties of aspergillus oryzae tannase.tannase (ec. from aspergillus oryzae was purified using ammonium sulphate precipitation (75%), gel filtration chromatography through sephadex g-100, and g-200. the purified enzyme was monomeric protein with a molecular mass of 106kda. the activation energy for tannic acid hydrolysis was 32.6kjmol(-1) and its temperature quotient (q10) was 1.0. the pka1 and pka2 values of acidic and basic limbs of the active site residues were 4.6 and 6.4. the calculated values of thermodynamic parameter ...201627373426
chirally pure prodrugs and their converting enzymes lead to high supersaturation and rapid transcellular permeation of benzodiazepines.water-soluble prodrugs can be rapidly converted by enzymes to hydrophobic drugs, whose aqueous thermodynamic solubilities are low, but are maintained in aqueous solution at supersaturated concentrations due to slow precipitation kinetics. recently, we investigated avizafone (avf) in combination with aspergillus oryzae protease as a prodrug/enzyme system intended to produce supersaturated diazepam (dzp). several fold enhancement of permeation of supersaturated dzp across madin-darby canine kidney ...201627342435
potential pitfalls associated with testing of enzyme preparations in the salmonella/microsome assay.the effect of a sample of food enzyme preparations on s9 activity was evaluated in bacterial mutation assays with the salmonella typhimurium strains ta98 and ta100 using benzo(a)pyrene, 2-aminoanthracene and 2-aminofluorene as model compounds. under the experimental conditions applied, aspergillus oryzae protease and porcine pancreas trypsin, applied at low non-toxic doses, proved to effectively inhibit the metabolic activation of benzo(a)pyrene by aroclor induced rat liver 9, while the activati ...201627330008
six independent fucose-binding sites in the crystal structure of aspergillus oryzae lectin.the crystal structure of aol (a fucose-specific lectin of aspergillus oryzae) has been solved by sad (single-wavelength anomalous diffraction) and mad (multi-wavelength anomalous diffraction) phasing of seleno-fucosides. the overall structure is a six-bladed β-propeller similar to that of other fucose-specific lectins. the fucose moieties of the seleno-fucosides are located in six fucose-binding sites. although the arg and glu/gln residues bound to the fucose moiety are common to all fucose-bind ...201627318092
metabolomic profiles of aspergillus oryzae and bacillus amyloliquefaciens during rice koji fermentation.rice koji, used early in the manufacturing process for many fermented foods, produces diverse metabolites and enzymes during fermentation. using gas chromatography time-of-flight mass spectrometry (gc-tof-ms), ultrahigh-performance liquid chromatography linear trap quadrupole ion trap tandem mass spectrometry (uhplc-ltq-it-ms/ms), and multivariate analysis we generated the metabolite profiles of rice koji produced by fermentation with aspergillus oryzae (rk_ao) or bacillus amyloliquefaciens (rk_ ...201627314317
a novel organic solvent- and detergent-stable serine alkaline protease from trametes cingulata strain ctm10101.a protease-producing fungus was isolated from an alkaline wastewater of chemical industries and identified as trametes cingulata strain ctm10101 on the basis of the its rdna gene-sequencing. it was observed that the fungus strongly produce extracellular protease grown at 30°c in potato-dextrose-broth (pdb) optimized media (13500u/ml). the pure serine protease isolated by trametes cingulata (designated sptc) was purified by ammonium sulfate precipitation-dialysis followed by heat-treatment and un ...201627296442
autolysis of aspergillus oryzae mycelium and effect on volatile flavor compounds of soy sauce.the autolyzed mycelia of aspergillus oryzae are rich in proteins, nucleic acids, sugar, and other biomacromolecules, and are one of the main contributors to the flavor profile of commercially important fermented goods, including soy sauce and miso. we induced autolysis of the mycelia of a. oryzae over 1 to 10 d, and found that the maximum dissolved amounts of total protein and nucleic acid ratio accounted for 28.63% and 88.93%, respectively. the organic acid content, such as citric acid, tartari ...201627464006
sequential mixed cultures: from syngas to malic acid.synthesis gas (syngas) fermentation using acetogenic bacteria is an approach for production of bulk chemicals like acetate, ethanol, butanol, or 2,3-butandiol avoiding the fuel vs. food debate by using carbon monoxide, carbon dioxide, and hydrogen from gasification of biomass or industrial waste gases. suffering from energetic limitations, yields of c4-molecules produced by syngas fermentation are quite low compared with abe fermentation using sugars as a substrate. on the other hand, fungal pro ...201627445993
using a flexible shaft agitator to enhance the rheology of a complex fungal fermentation culture.the rheology behavior of biological fluids particularly when the viscosity is high and rheology is complex, is an important issue to understand, particularly for studies in mass-transfer and for solving technical problems with mixing in stirred bioreactors. in this paper, the use of a swingstir(®) impeller during the fermentation of aspergillus oryzae resulted in decreases from the parameters of a power-law model, in viscosity and in the thixotropic behavior of a cultivation broth. the results s ...201627438373
effect of mechanical denaturation on surface free energy of protein powders.globular proteins are important both as therapeutic agents and excipients. however, their fragile native conformations can be denatured during pharmaceutical processing, which leads to modification of the surface energy of their powders and hence their performance. lyophilized powders of hen egg-white lysozyme and β-galactosidase from aspergillus oryzae were used as models to study the effects of mechanical denaturation on the surface energies of basic and acidic protein powders, respectively. t ...201627434157
molecular cloning and characterization of a novel bi-functional α-amylase/subtilisin inhibitor from hevea brasiliensis.a novel cdna encoding a bi-functional α-amylase/subtilisin inhibitor (hbasi) was isolated from rubber (hevea brasiliensis) leaves cultivar rrim600. the hbasi had strong homology with the soybean trypsin inhibitor (kunitz) family of protease inhibitors. its putative amino acid sequence was similar to that of the α-amylase/subtilisin inhibitor from ricinus communis (72% identity). genomic sequencing indicated that the hbasi gene contained no introns. the messenger rna of hbasi was detected in leaf ...201626854410
efficient kinetic resolution of (rs)-1-phenylethanol by a mycelium-bound lipase from a wild-type aspergillus oryzae strain.a mycelium-bound lipase from aspergillus oryzae (aol) exhibited excellent enantioselectivity for kinetic resolution of (rs)-1-phenylethanol in organic solvent. the various reaction parameters affecting the conversion and enantioselectivity were studied, including type of acyl donor, solvent, molar ratio, temperature, enzyme amount and substrate concentration. the optimum reaction conditions were found to be transesterification with vinyl acetate at 30°c in methyl tert-butyl ether with a vinyl ac ...201626854002
comparison of catalytic properties of multiple β-glucosidases of trichoderma reesei.ten putative trichoderma reesei β-glucosidase (bgl) isozymes were heterologously expressed in escherichia coli and aspergillus oryzae and purified to homogeneity. catalytic properties of nine enzymes which showed hydrolytic activity on cellobiose and p-nitrophenyl-β-d-glucopyranoside (pnpg) were investigated. three bgls, encoded by the genes cel3a, cel3b, and cel3e, contained a predicted signal peptide, showed higher hydrolytic activity on cello-oligosaccharides than on pnpg, and preferred longe ...201626846743
antiviral and cytotoxic isocoumarin derivatives from an endophytic fungus aspergillus oryzae.oryzaeins a-d (1-4), four new isocoumarin derivatives, along with five known ones (5-9) were isolated from solid cultures of an endophytic fungus aspergillus oryzae. their structures were elucidated by detailed spectroscopic analysis and by comparison with reported data of related derivatives. among them, compounds 1 and 2 represent the first examples of isocoumarins possessing an unusual 2-oxopropyl group and a rare 3-hydroxypropyl group. compounds 1 and 2 displayed moderate anti-tobacco mosaic ...201626824624
anti-obesity effect of extract from fermented curcuma longa l. through regulation of adipogenesis and lipolysis pathway in high-fat diet-induced obese rats.even though curcuma longa l. possesses various biological activities, it has strong flavor and taste, which decrease consumer palatability and limit industrial applications in food.201626822962
optimization of liquid-state fermentation conditions for the glyphosate degradation enzyme production of strain aspergillus oryzae by ultraviolet mutagenesis.this study aimed to obtain strains with high glyphosate-degrading ability and improve the ability of glyphosate degradation enzyme by the optimization of fermentation conditions. spore from aspergillus oryzae a-f02 was subjected to ultraviolet mutagenesis. single-factor experiment and response surface methodology were used to optimize glyphosate degradation enzyme production from mutant strain by liquid-state fermentation. four mutant strains were obtained and named as fujx 001, fujx 002, fujx 0 ...201626795747
aspergillus oryzae-saccharomyces cerevisiae consortium allows bio-hybrid fuel cell to run on complex carbohydrates.consortia of aspergillus oryzae and saccharomyces cerevisiae are examined for their abilities to turn complex carbohydrates into ethanol. to understand the interactions between microorganisms in consortia, fourier-transform infrared spectroscopy is used to follow the concentrations of various metabolites such as sugars (e.g., glucose, maltose), longer chain carbohydrates, and ethanol to optimize consortia conditions for the production of ethanol. it is shown that with proper design a. oryzae can ...201627681904
valorization of sugar-to-ethanol process waste vinasse: a novel biorefinery approach using edible ascomycetes filamentous fungi.the aim of the present work was to study the integration of edible ascomycetes filamentous fungi into the existing sugar- or molasses-to-ethanol processes, to grow on vinasse or stillage and produce ethanol and protein-rich fungal biomass. two fungal strains, neurospora intermedia and aspergillus oryzae were examined in shake flasks and airlift-bioreactors, resulting in reduction of vinasse cod by 34% and viscosity by 21%. utilization of glycerol and sugars were observed, yielding 202.4 or 222.8 ...201627668880
fungal fermentation of lignocellulosic biomass for itaconic and fumaric acid production.the production of high-value chemicals from natural resources as alternative of petroleum-based products is currently expanding in parallel with biorefinery. the use of lignocellulosic biomass as raw material is promising to achieve economic and environmental sustainability. filamentous fungi, particularly aspergillus species, are already used industrially to produce organic acid as well as many enzymes. the production of lignocellulose degrading enzymes opens the possibility for direct fungal f ...201627666988
effect of different amylases on the utilization of cornstarch in broiler chickens.this study compared the effect of different amylases on the utilization of cornstarch in broiler chickens fed a corn-based diet. three-day-old arbor acres plus male chickens were randomly divided into 7 treatments and fed a diet supplemented with different sources and concentrations of amylase: 1,500 u/kg and 3,000 u/kg α-1,4 amylase from aspergillus oryzae (α-amylase a); 480 u/kg and 960 u/kg α-1,4 amylase from bacillus subtilis (α-amylase b); 200 u/kg and 400 u/kg α-1,6 isoamylase from b. subt ...201627664199
biosynthesis of shearinine: diversification of a tandem prenyl moiety of fungal indole diterpenes.the late-stage biosynthetic pathway of the indole diterpene shearinine involving four enzymatic reactions (janqdoj) was elucidated by an efficient heterologous expression system using aspergillus oryzae. key oxidative cyclization, forming a characteristic a/b bicyclic shearinine core by flavoprotein oxidase, was studied using a substrate analogue and a buffer containing h2(18)o. these experimental data provided evidence that jano catalyzes two-step oxidation via a hydroxylated product and that t ...201627632559
identification of a novel sesquiterpene biosynthetic machinery involved in astellolide biosynthesis.esterified drimane-type sesquiterpene lactones such as astellolides display various biological activities and are widely produced by plants and fungi. given their low homology to known sesquiterpene cyclases, the genes responsible for their biosynthesis have not been uncovered yet. here, we identified the astellolide gene cluster from aspergillus oryzae and discovered a novel sesquiterpene biosynthetic machinery consisting of astc, asti, and astk. all these enzymes are annotated as haloacid deha ...201627628599
optimized expression of prolyl aminopeptidase in pichia pastoris and its characteristics after glycosylation.prolyl aminopeptidases are specific exopeptidases that catalyze the hydrolysis of the n-terminus proline residue of peptides and proteins. in the present study, the prolyl aminopeptidase gene (pap) from aspergillus oryzae jn-412 was optimized through the codon usage of pichia pastoris. both the native and optimized pap genes were inserted into the expression vector ppic9 k and were successfully expressed in p. pastoris. additionally, the activity of the intracellular enzyme expressed by the reco ...201627628336
regioselective dichlorination of a non-activated aliphatic carbon atom and phenolic bismethylation by a multifunctional fungal flavoenzyme.the regioselective functionalization of non-activated carbon atoms such as aliphatic halogenation is a major synthetic challenge. a novel multifunctional enzyme catalyzing the geminal dichlorination of a methyl group was discovered in aspergillus oryzae (koji mold), an important fungus that is widely used for asian food fermentation. a biosynthetic pathway encoded on two different chromosomes yields mono- and dichlorinated polyketides (diaporthin derivatives), including the cytotoxic dichlorodia ...201627559694
artificial modification of an enzyme for construction of cross-reactive polyion complexes to fingerprint signatures of proteins and mammalian cells.a novel strategy for construction of cross-reactive enzyme-based sensors have been developed based on chemical modification of lysine ε-nh3(+) groups in β-galactosidase (β-gal) from aspergillus oryzae with various acid anhydrides. modification of lysine side chains markedly altered the kinetic parameters of β-gal (km and kcat), whereas catalytic activity remained and the tertiary structure was maintained for all modified β-gals. the addition of cationic pegylated polymers to reactive solutions c ...201627548408
evaluation of commercial soy sauce koji strains of aspergillus oryzae for γ-aminobutyric acid (gaba) this study, four selected commercial strains of aspergillus oryzae were collected from soy sauce koji. these a. oryzae strains designated as nsk, nsz, nsj and nst shared similar morphological characteristics with the reference strain (a. oryzae frr 1675) which confirmed them as a. oryzae species. they were further evaluated for their ability to produce γ-aminobutyric acid (gaba) by cultivating the spore suspension in a broth medium containing 0.4 % (w/v) of glutamic acid as a substrate for ga ...201627541157
overproduction, purification and characterization of adenylate deaminase from aspergillus oryzae.adenylate deaminase (ampd, ec is an aminohydrolase that widely used in the food and medicine industries. in this study, the gene encoding aspergillus oryzae ampd was cloned and expressed in escherichia coli. induction with 0.75 mm isopropyl β-d-l-thiogalactopyranoside resulted in an enzyme activity of 1773.9 u/ml. recombinant ampd was purified to electrophoretic homogeneity using nickel affinity chromatography, and its molecular weight was calculated as 78.6 kda. purified ampd exhibited ...201627539573
expression, purification and identification of a thermolysin-like protease, neutral protease i, from aspergillus oryzae with the pichia pastoris expression system.neutral proteases are widely used in the textile, food and medical industries. this study was designed to obtain high expression levels of neutral protease i from aspergillus oryzae 3.042 by using pichia pastoris gs115 as the host strain for industrial purposes. the coding sequence of the target gene was modified, synthesized, and then cloned into the expression vector phbm905bdm, which harbored the d1+2 × 201 aox1 promoter and the mf4i leader sequence. the recombinant plasmid was transformed in ...201627539551
functional and transcriptomic analysis of the key unfolded protein response transcription factor haca in aspergillus oryzae.haca is a conserved basic leucine zipper transcription factor that serves as the master transcriptional regulator in the unfolded protein response (upr). to comprehensively evaluate the role of haca in aspergillus oryzae, a homokaryotic haca disruption mutant (haca-de) and a strain that expressed a constitutively active form of haca (haca-ca) were successfully generated, and transcriptome analyses of these mutants were performed. growth and phenotypic profiles demonstrated that hyphal growth and ...201627520586
japanese traditional dietary fungus koji aspergillus oryzae functions as a prebiotic for blautia coccoides through glycosylceramide: japanese dietary fungus koji is a new prebiotic.the japanese traditional cuisine, washoku, considered to be responsible for increased longevity among the japanese, comprises various foods fermented with the non-pathogenic fungus aspergillus oryzae (koji). we have recently revealed that koji contains an abundant amount of glycosylceramide. intestinal microbes have significant effect on health. however, the effects of koji glycosylceramide on intestinal microbes have not been studied.201627563516
screening for antimicrobial activity of wood rotting higher basidiomycetes mushrooms from uruguay against this work, the antimicrobial activity of extracts of wood rotting higher basidiomycetes mushrooms isolated from eucalyptus plantations in uruguay was studied using bacterial and fungal phytopathogens as targets. fifty-one extracts from mycelia and growth broth were prepared from higher basidiomycetes mushrooms, from which eight extracts (from ganoderma resinaceum, laetiporus sulphureus, dictyopanus pusillus, and bjerkandera adusta) showed antimicrobial activity against xanthomonas vesicatoria ...201627481160
aberrant glycosylation in the left ventricle and plasma of rats with cardiac hypertrophy and heart failure.targeted proteomics focusing on post-translational modifications, including glycosylation, is a useful strategy for discovering novel biomarkers. to apply this strategy effectively to cardiac hypertrophy and resultant heart failure, we aimed to characterize glycosylation profiles in the left ventricle and plasma of rats with cardiac hypertrophy. dahl salt-sensitive hypertensive rats, a model of hypertension-induced cardiac hypertrophy, were fed a high-salt (8% nacl) diet starting at 6 weeks. as ...201627281159
development of a promoter shutoff system in aspergillus oryzae using a sorbitol-sensitive promoter.promoter shutoff is a general method for analyzing essential genes, but in the fungus aspergillus oryzae, no tightly repressed promoters have been reported. to overcome the current limitations of conditional promoters, we examined sorbitol- and galactose-responsive genes using microarrays to identify regulatable genes with only minor physiological and genetic effects. we identified two sorbitol-induced genes (designated as sora and sorb), cloned their promoters, and built a regulated egfp and br ...201627280333
sequence- and structure-based functional annotation and assessment of metabolic transporters in aspergillus oryzae: a representative case study.aspergillus oryzae is widely used for the industrial production of enzymes. in a. oryzae metabolism, transporters appear to play crucial roles in controlling the flux of molecules for energy generation, nutrients delivery, and waste elimination in the cell. while the a. oryzae genome sequence is available, transporter annotation remains limited and thus the connectivity of metabolic networks is incomplete. in this study, we developed a metabolic annotation strategy to understand the relationship ...201627274991
modeling lactose hydrolysis for efficiency and selectivity: toward the preservation of sialyloligosaccharides in bovine colostrum whey permeate.enzymatic hydrolysis of lactose has been shown to improve the efficiency and selectivity of membrane-based separations toward the recovery of bioactive oligosaccharides. achieving maximum lactose hydrolysis requires intrinsic process optimization for each specific substrate, but the effects of those processing conditions on the target oligosaccharides are not well understood. response surface methodology was used to investigate the effects of ph (3.25-8.25), temperature (35-55°c), reaction time ...201627236766
nematicidal activity of kojic acid produced by aspergillus oryzae against meloidogyne incognita.the fungal strain eml-dml3pna1 isolated from leaf of white dogwood (cornus alba l.) showed strong nematicidal activity with juvenile mortality of 87.6% at a concentration of 20% fermentation broth filtrate at 3 days after treatment. the active fungal strain was identified as aspergillus oryzae, which belongs to section flavi, based on the morphological characteristics and sequence analysis of the its rdna, calmodulin (cam), and β-tubulin (bena) genes. the strain reduced the ph value to 5.62 afte ...201627197670
development of enzyme technology for aspergillus oryzae, a. sojae, and a. luchuensis, the national microorganisms of japan.this paper describes the modern enzymology in japanese bioindustries. the invention of takadiastase by jokiti takamine in 1894 has revolutionized the world of industrial enzyme production by fermentation. in 1949, a new γ-amylase (glucan 1,4-α-glucosidase, ec from a. luchuensis (formerly designated as a. awamori), was found by kitahara. rnase t1 (guanyloribonuclease, ec was discovered by sato and egami. ando discovered aspergillus nuclease s1 (single-stranded nucleate endonucl ...201627151561
identification and manipulation of the pleuromutilin gene cluster from clitopilus passeckerianus for increased rapid antibiotic production.semi-synthetic derivatives of the tricyclic diterpene antibiotic pleuromutilin from the basidiomycete clitopilus passeckerianus are important in combatting bacterial infections in human and veterinary medicine. these compounds belong to the only new class of antibiotics for human applications, with novel mode of action and lack of cross-resistance, representing a class with great potential. basidiomycete fungi, being dikaryotic, are not generally amenable to strain improvement. we report identif ...201627143514
highly efficient fructooligosaccharides production by an erythritol-producing yeast yarrowia lipolytica displaying fructosyltransferase.currently, fructooligosaccharides (fos) are industrially transformed from sucrose by purified enzymes or fungi cells. however, these methods are expensive and time-consuming. an economical approach to producing fos using erythritol-producing yeast cells was described in this study. fructosyltransferase from aspergillus oryzae was displayed on the cell surface of yarrowia lipolytica, resulting in an engineered strain capable of transforming sucrose to fos. an amount of 480 g/l fos was produced wi ...201627124471
structural elucidation of enzymatically synthesized galacto-oligosaccharides using ion-mobility spectrometry-tandem mass spectrometry.galacto-oligosaccharides (gos) represent a diverse group of well-characterized prebiotic ingredients derived from lactose in a reaction catalyzed with β-galactosidases. enzymatic transgalactosylation results in a mixture of compounds of various degrees of polymerization and types of linkages. because structure plays an important role in terms of prebiotic activity, it is of crucial importance to provide an insight into the mechanism of transgalactosylation reaction and occurrence of different ty ...201627109424
molecular dissection of so (soft) protein in stress-induced aggregation and cell-to-cell interactive functions in filamentous fungal multicellularity.filamentous fungi grow by organizing multicellularity through hyphal compartmentalization and cell fusion. so (soft) protein, which was originally identified in neurospora crassa, plays distinct functional roles in cell-to-cell interactions, such as septal plugging and cell fusion. we previously reported that aoso, an aspergillus oryzae so homologue, forms aggregates at the septal pore in response to stress, as well as upon hyphal wounding. however, the functional regions that mediate the multic ...201627109373
aspergillus oryzae nrta affects kojic acid production.we analyzed the role of the nitrate transporter-encoding gene (nrta) of aspergillus oryzae by gene disruption. southern hybridization analysis indicated that homologous recombination occurred at the resident nrta locus. real-time pcr showed that the nrta gene was strongly inducible by nano3. the nrta disruptant did not exhibit normal growth when nitrate was available as the sole nitrogen source. these results indicate that nrta is essential for nitrate uptake in a. oryzae. kojic acid (ka) produc ...201627108780
aspergillus oryzae s2 α-amylase domain c involvement in activity and specificity: in vivo proteolysis, molecular and docking studies.we previously reported that aspergillus oryzae strain s2 had produced two α-amylase isoforms named amya and amyb. the apparent molecular masses revealed by sds-page were 50 and 42 kda, respectively. yet amyb has a higher catalytic efficiency. based on a monitoring study of the α-amylase production in both the presence and absence of different protease inhibitors, a chymotrypsin proteolysis process was detected in vivo generating amyb. a. oryzae s2 α-amylase gene was amplified, cloned and sequenc ...201627101008
an alternative approach to synthesizing galactooligosaccharides by cell-surface display of β-galactosidase on yarrowia alternative strategy for synthesizing galactooligosaccharides (gos) from an erythritol-producing yeast yarrowia lipolytica using surface display technology was demonstrated. the engineered strain cgmcc11369 was developed by fusion of the β-galactosidase gene from aspergillus oryzae to the ylpir1 gene, which codes for a cell wall protein. β-galactosidase was effectively displayed on the cell surface of yarrowia lipolytica start strain cgmcc7326. this engineered strain with surface-displayed β- ...201627090877
production of alpha-amylase from aspergillus oryzae for several industrial applications in a single step.a one-step method as a strategy of alpha-amylase concentration and purification was developed in this work. this methodology requires the use of a very low concentration of biodegradable polyelectrolyte (eudragit(®) e-po) and represents a low cost, fast, easy to scale up and non-polluting technology. besides, this methodology allows recycling the polymer after precipitation. the formation of reversible soluble/insoluble complexes between alpha-amylase and the polymer eudragit(®) e-po was studied ...201627085017
biotransformation of l-tyrosine to dopamine by a calcium alginate immobilized mutant strain of aspergillus oryzae.the present research work is concerned with the biotransformation of l-tyrosine to dopamine (da) by calcium alginate entrapped conidiospores of a mutant strain of aspergillus oryzae. different strains of a. oryzae were isolated from soil. out of 13 isolated strains, isolate-2 (i-2) was found to be a better da producer. the wild-type i-2 was chemically improved by treating it with different concentrations of ethyl methyl sulfonate (ems). among seven mutant variants, ems-6 exhibiting maximal da ac ...201627068833
a novel non-thermostable deuterolysin from aspergillus oryzae.three putative deuterolysin (ec genes (deua, deub, and deuc) were found in the aspergillus oryzae genome database ( ). one of these genes, deua, was corresponding to npii gene, previously reported. deua and deub were overexpressed by recombinant a. oryzae and were purified. the degradation profiles against protein substrates of both enzymes were similar, but deub showed wider substrate specificity against peptidyl mca-substrates compared ...201627050120
astellifadiene: structure determination by nmr spectroscopy and crystalline sponge method, and elucidation of its biosynthesis.genome mining of a terpene synthase gene from emericella variecolor nbrc 32302 and its functional expression in aspergillus oryzae led to the production of the new sesterterpene hydrocarbon, astellifadiene (1), having a 6-8-6-5-fused ring system. the structure of 1 was initially investigated by extensive nmr analyses, and was further confirmed by the crystalline sponge method, which established the absolute structure of 1 and demonstrated the usefulness of the method in the structure determinati ...201627038368
enhancement of fructosyltransferase and fructooligosaccharides production by a. oryzae dia-mf in solid-state fermentation using aguamiel as culture medium.the aim of this work was to improve the production of fructosyltransferase (ftase) by solid-state fermentation (ssf) using aguamiel (agave sap) as culture medium and aspergillus oryzae dia-mf as producer strain. ssf was carried out evaluating the following parameters: inoculum rate, incubation temperature, initial ph and packing density to determine the most significant factors through box-hunter and hunter design. the significant factors were then further optimized using a box-behnken design an ...201627036329
synthesis of oligosaccharides derived from lactulose (oslu) using soluble and immobilized aspergillus oryzae β-galactosidase.β-galactosidase from aspergillus oryzae offers a high yield for the synthesis of oligosaccharides derived from lactulose (oslu) by transgalactosylation. oligosaccharides with degree of polymerization (dp) ≥ 3 have shown to possess higher in vitro bifidogenic effect than di- and tetrasaccharides. thus, in this work, an optimization of reaction conditions affecting the specific selectivity of a. oryzae β-galactosidase for synthesis of oslu has been carried out to enhance oslu with dp ≥ 3 productio ...201627014684
further increased production of free fatty acids by overexpressing a predicted transketolase gene of the pentose phosphate pathway in aspergillus oryzae faaa fatty acids are useful as source materials for the production of biodiesel fuel and various chemicals such as pharmaceuticals and dietary supplements. previously, we attained a 9.2-fold increase in free fatty acid productivity by disrupting a predicted acyl-coa synthetase gene (faaa, ao090011000642) in aspergillus oryzae. in this study, we achieved further increase in the productivity by overexpressing a predicted transketolase gene of the pentose phosphate pathway in the faaa disruptant. t ...201626998626
substantial decrease in cell wall α-1,3-glucan caused by disruption of the kexb gene encoding a subtilisin-like processing protease in aspergillus oryzae.disruption of the kexb encoding a subtilisin-like processing protease in aspergillus oryzae (δkexb) leads to substantial morphological defects when the cells are grown on czapek-dox agar plates. we previously found that the disruption of kexb causes a constitutive activation of the cell wall integrity pathway. to understand how the disruption of the kexb affects cell wall organization and components, we analyzed the cell wall of δkexb grown on the plates. the results revealed that both total n-a ...201626980104
the c2h2-type transcription factor, flbc, is involved in the transcriptional regulation of aspergillus oryzae glucoamylase and protease genes specifically expressed in solid-state culture.aspergillus oryzae produces a large amount of secreted proteins in solid-state culture, and some proteins such as glucoamylase (glab) and acid protease (pepa) are specifically produced in solid-state culture, but rarely in submerged culture. from the disruption mutant library of a. oryzae transcriptional regulators, we successfully identified a disruption mutant showing an extremely low production level of glab but a normal level of α-amylase production. this strain was a disruption mutant of th ...201626960315
performance of an ultrafiltration membrane bioreactor (uf-mbr) as a processing strategy for the synthesis of galacto-oligosaccharides at high substrate concentrations.the performance of an ultrafiltration membrane bioreactor for galacto-oligosaccharides (gos) synthesis using high lactose concentrations (470 g/l) and β-galactosidase from aspergillus oryzae was assessed. tested processing variables were: transmembrane-pressure (pt), crossflow-velocity (cfv) and temperature. results showed that processing variables had significant effect on the yield, the enzyme productivity and the flux but did not on gos concentration and reaction conversion obtained. as expec ...201626924238
occupational sensitization to lactase in the dietary supplement industry.aerogen lactase exposure carries a risk for the development of allergic asthma and rhinitis; only a few occupationally affected patients have been reported. the authors report the results of allergy testing with employees of a lactase tablets manufacturing plant. the survey involved 13 workers, including a questionnaire, spirometry, basophil activation test (bat), and skin prick tests (spts) with lactase and a panel of common aeroallergens. furthermore, lactase-specific immunoglobulin e (ige) an ...201626134755
aorim15 is involved in conidial stress tolerance, conidiation and sclerotia formation in the filamentous fungus aspergillus oryzae.the serine-threonine kinase rim15p is a master regulator of stress signaling and is required for stress tolerance and sexual sporulation in the yeast saccharomyces cerevisiae. however, in filamentous fungi that reproduce asexually via conidiation, the physiological function of rim15p homologs has not been extensively analyzed. here, we functionally characterized the protein homolog of rim15p in the filamentous fungus aspergillus oryzae, by deleting and overexpressing the corresponding aorim15 ge ...201626467693
toward rational thermostabilization of aspergillus oryzae cutinase: insights into catalytic and structural stability.cutinases are powerful hydrolases that can cleave ester bonds of polyesters such as poly(ethylene terephthalate) (pet), opening up new options for enzymatic routes for polymer recycling and surface modification reactions. cutinase from aspergillus oryzae (aoc) is promising owing to the presence of an extended groove near the catalytic triad which is important for the orientation of polymeric chains. however, the catalytic efficiency of aoc on rigid polymers like pet is limited by its low thermos ...201626522152
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