Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| lactose hydrolysis in milk and dairy whey using microbial β-galactosidases. | this work aimed at evaluating the influence of enzyme concentration, temperature, and reaction time in the lactose hydrolysis process in milk, cheese whey, and whey permeate, using two commercial β-galactosidases of microbial origins. we used aspergillus oryzae (at temperatures of 10 and 55°c) and kluyveromyces lactis (at temperatures of 10 and 37°c) β-galactosidases, both in 3, 6, and 9 u/ml concentrations. in the temperature of 10°c, the k. lactis β-galactosidase enzyme is more efficient in th ... | 2015 | 26587283 |
| deciphering the signaling mechanisms of the plant cell wall degradation machinery in aspergillus oryzae. | the gene expression and secretion of fungal lignocellulolytic enzymes are tightly controlled at the transcription level using independent mechanisms to respond to distinct inducers from plant biomass. an advanced systems-level understanding of transcriptional regulatory networks is required to rationally engineer filamentous fungi for more efficient bioconversion of different types of biomass. | 2015 | 26573537 |
| [novel type iii polyketide synthase csyb from aspergillus oryzae]. | 2015 | 26571621 | |
| molecular characterization of a penicillium chrysogenum exo-rhamnogalacturonan lyase that is structurally distinct from other polysaccharide lyase family proteins. | we previously described an endo-acting rhamnogalacturonan (rg) lyase, termed pcrgl4a, of penicillium chrysogenum 31b. here, we describe a second rg lyase, called pcrglx. we determined the cdna sequence of the pcrglx gene, which encodes pcrglx. based on analyses using a blast search and a conserved domain search, pcrglx was found to be structurally distinct from known rg lyases and might belong to a new polysaccharide lyase family together with uncharacterized fungal proteins of nectria haematoco ... | 2015 | 25921806 |
| hybrid de novo genome assembly using miseq and solid short read data. | a hybrid de novo assembly pipeline was constructed to utilize both miseq and solid short read data in combination in the assembly. the short read data were converted to a standard format of the pipeline, and were supplied to the pipeline components such as abyss and soapdenovo. the assembly pipeline proceeded through several stages, and either miseq paired-end data, solid mate-paired data, or both of them could be specified as input data at each stage separately. the pipeline was examined on the ... | 2015 | 25919614 |
| signal peptide optimization tool for the secretion of recombinant protein from saccharomyces cerevisiae. | the secretion efficiency of foreign proteins in recombinant microbes is strongly dependent on the combination of the signal peptides (sps) used and the target proteins; therefore, identifying the optimal sp sequence for each target protein is a crucial step in maximizing the efficiency of protein secretion in both prokaryotes and eukaryotes. in this study, we developed a novel method, named the sp optimization tool (spot), for the generation and rapid screening of a library of sp-target gene fus ... | 2015 | 25912446 |
| galacto configured n-aminoaziridines: a new type of irreversible inhibitor of β-galactosidases. | a new type of galactose mimetics has been synthesized following a straightforward synthetic approach based on cyclohexene olefin aziridination reactions directed by hydroxyl substituents. these enantiomerically pure galacto-configured n-aminoaziridines are potent irreversible inhibitors of aspergillus oryzae and escherichia coliβ-galactosidases. | 2015 | 25895752 |
| survey of protein-dna interactions in aspergillus oryzae on a genomic scale. | the genome-scale delineation of in vivo protein-dna interactions is key to understanding genome function. only ∼5% of transcription factors (tfs) in the aspergillus genus have been identified using traditional methods. although the aspergillus oryzae genome contains >600 tfs, knowledge of the in vivo genome-wide tf-binding sites (tfbss) in aspergilli remains limited because of the lack of high-quality antibodies. we investigated the landscape of in vivo protein-dna interactions across the a. ory ... | 2015 | 25883143 |
| preventive effects of fermented brown rice and rice bran against n-nitrosobis (2-oxopropyl) amine-induced pancreatic tumorigenesis in male hamsters. | fermented brown rice by aspergillus oryzae (fbra) is known to have the potential to prevent chemical carcinogenesis of the colon, liver, esophagus, urinary bladder, stomach and lungs in rodents. the present study examined the possible chemopreventive effects of fbra on n-nitrosobis(2-oxopropyl)amine (bop)-induced pancreatic tumorigenesis in hamsters. five-week-old male syrian golden hamsters were divided into seven groups. groups 1-5 were subcutaneously injected with bop (10 mg/kg body weight) f ... | 2015 | 26788138 |
| fermented brown rice and rice bran with aspergillus oryzae (fbra) prevents inflammation-related carcinogenesis in mice, through inhibition of inflammatory cell infiltration. | we have established an inflammation-related carcinogenesis model in mouse, in which regressive qr-32 cells subcutaneously co-implanted with a foreign body-gelatin sponge-convert themselves into lethal tumors due to massive infiltration of inflammatory cells into the sponge. animals were fed with a diet containing 5% or 10% fermented brown rice and rice bran with aspergillus oryzae (fbra). in 5% and 10% fbra diet groups, tumor incidences were lower (35% and 20%, respectively) than in the non-trea ... | 2015 | 26670250 |
| towards effective and stable probiotics. | probiotics are live microorganisms, generally either lactobacilli or bifidobacteria, which when administered in adequate amounts confer a health benefit to the host [1]. due to the growing evidence of health benefits associated with their use, probiotics are of increasing interest and represent now a significant growth area in the functional foods industry [2]. however, to be effective, orally administered probiotics should survive preparation of dosage forms and passage through acidic environme ... | 2015 | 26639716 |
| effect of neohesperidin dihydrochalcone on the activity and stability of alpha-amylase: a comparative study on bacterial, fungal, and mammalian enzymes. | neohesperidin dihydrochalcone (nhdc) was recently introduced as an activator of mammalian alpha-amylase. in the current study, the effect of nhdc has been investigated on bacterial and fungal alpha-amylases. enzyme assays and kinetic analysis demonstrated the capability of nhdc to significantly activate both tested alpha-amylases. the ligand activation pattern was found to be more similar between the fungal and mammalian enzyme in comparison with the bacterial one. further, thermostability exper ... | 2015 | 25808616 |
| aspergillus oryzae pathways that convert phenylalanine into the flavor volatile 2-phenylethanol. | the filamentous fungus aspergillus oryzae rib40 produced 2-phenylethanol (pe) when cultured in minimum medium containing l-phenylalanine as a sole source of nitrogen. the fungus accumulated less pe in the absence of l-phenylalanine, indicating that it converted l-phenylalanine to pe. the pe production associated with fungal glucose consumption was repressed by exogenous ammonium, indicating that nitrogen-metabolite repression controls the pathway that produces pe. we identified the a. oryzae ppd ... | 2015 | 25797315 |
| effective saccharification of kraft pulp by using a cellulase cocktail prepared from genetically engineered aspergillus oryzae. | kraft pulp is a promising feedstock for bioproduction. the efficiency of kraft pulp saccharification was improved by using a cellulase cocktail prepared from genetically engineered aspergillus oryzae. application of the cellulase cocktail was demonstrated by simultaneous saccharification and fermentation, using kraft pulp and non-cellulolytic yeast. such application would make possible to do an efficient production of other chemicals from kraft pulp. | 2015 | 25783466 |
| cell biology of the koji mold aspergillus oryzae. | koji mold, aspergillus oryzae, has been used for the production of sake, miso, and soy sauce for more than one thousand years in japan. due to the importance, a. oryzae has been designated as the national micro-organism of japan (koku-kin). a. oryzae has been intensively studied in the past century, with most investigations focusing on breeding techniques and developing methods for koji making for sake brewing. however, the understanding of fundamental biology of a. oryzae remains relatively lim ... | 2015 | 25776568 |
| product identification of non-reducing polyketide synthases with c-terminus methyltransferase domain from talaromyces stipitatus using aspergillus oryzae heterologous expression. | talaromyces stipitatus atcc 10500 possesses 17 non-reducing polyketide synthase (nr-pks) genes. during the course of our functional analysis of pks genes with a c-terminus methyltransferase domain from t. stipitatus, we expressed tspks2, tspks3 and tspks4 genes in the heterologous host aspergillus oryzae, respectively. although the tspks4 transformant gave no apparent product in hplc analysis, a novel azaphilone pentaketide (3) was identified along with two known related products from the tspks2 ... | 2015 | 25770780 |
| drimane sesquiterpenoids from the aspergillus oryzae qxpc-4. | three new drimane sesquiterpenoids, astellolides c-e (1-3, resp.), four new drimane sesquiterpenoid p-hydroxybenzoates, astellolides f-i (4-7, resp.), together with two known compounds astellolides a and b (8 and 9, resp.), have been isolated from the liquid culture of aspergillus oryzae (strain no. qxpc-4). their structures were established by comprehensive analysis of spectroscopic data. the relative and absolute configurations were determined on the basis of noesy and cd data, together with s ... | 2015 | 25766910 |
| identification of functional cis-elements required for repression of the taka-amylase a gene under secretion stress in aspergillus oryzae. | the expression of secreted proteins in filamentous fungi is down-regulated by a transcriptional feedback mechanism under endoplasmic reticulum stress, termed repression under secretion stress (ress). to investigate the ress mechanism, we analyzed the expression of the taka-amylase a gene (amyb) in aspergillus oryzae, which was depressed under secreted protein stress. we conducted a truncation and deletion analysis of the amyb promoter to identify cis-elements required for ress. a nucleotide sequ ... | 2015 | 25280730 |
| modulating endoplasmic reticulum-golgi cargo receptors for improving secretion of carrier-fused heterologous proteins in the filamentous fungus aspergillus oryzae. | filamentous fungi are excellent hosts for industrial protein production due to their superior secretory capacity; however, the yield of heterologous eukaryotic proteins is generally lower than that of fungal or endogenous proteins. although activating protein folding machinery in the endoplasmic reticulum (er) improves the yield, the importance of intracellular transport machinery for heterologous protein secretion is poorly understood. here, using aspergillus oryzae as a model filamentous fungu ... | 2015 | 25362068 |
| isolation and expression of enolase gene in fusarium oxysporum f. sp. lycopersici. | fusarium oxysporum f. sp. lycopersici is a fungus responsible for the tomato disease known as fusariosis. enolase, which is the enzyme that catalyzes the reaction of 2-phosphoglycerate to phosphoenolpyruvate, is present during glycolysis. enolase genes have been isolated from bacteria and fungi, among other organisms. in this research, a large portion of the enolase, eno, gene sequence was isolated from f. oxysporum and compared with those of other microorganisms, revealing a similarity of 51-69 ... | 2015 | 25349089 |
| characterization of the production of biogenic amines and gamma-aminobutyric acid in the soybean pastes fermented by aspergillus oryzae and lactobacillus brevis. | the production of gamma-aminobutyric acid (gaba) using gaba-producing lactic acid bacteria (lab) has been considered to be an attractive strategy. however, some lab may produce biogenic amines (ba), which may be of concern from the safety viewpoint. the aim of the present study was to characterize the production of gaba and ba in the soybean pastes fermented by aspergillus oryzae (a. oryzae) fmb s46471 and gaba-producing lactobacillus brevis (l. brevis) gaba 100. after a ripening period of 90 da ... | 2015 | 25341471 |
| fungal hemicellulose-degrading enzymes cause physical property changes concomitant with solubilization of cell wall polysaccharides. | physical properties of wheat coleoptile segments decreased after treatment with hemicellulose-degrading enzymes, indicating that hemicellulosic polysaccharides function to control the strength of primary cell walls. changes in the physical properties of plant cell walls, a viscoelastic structure, are thought to be one of the growth-limiting factors for plants and one of the infection-affecting factors for fungi. to study the significance of hemicellulosic polysaccharides that form cross-bridges ... | 2015 | 25301670 |
| a combination of acid lactase from aspergillus oryzae and yogurt bacteria improves lactose digestion in lactose maldigesters synergistically: a randomized, controlled, double-blind cross-over trial. | lactose digestion can be improved in subjects with impaired or completely absent intestinal lactase activity by administration of lactase preparations and particularly of acid lactase, which is active in the stomach, or by yogurt containing live lactic acid bacteria. it is the question, if lactose digestion can be further enhanced by combining these two approaches. | 2015 | 25042846 |
| c7-prenylation of tryptophanyl and o-prenylation of tyrosyl residues in dipeptides by an aspergillus terreus prenyltransferase. | during our search for novel prenyltransferases, a putative gene ateg_04218 from aspergillus terreus raised our attention and was therefore amplified from strain dsm 1958 and expressed in escherichia coli. biochemical investigations with the purified recombinant protein and different aromatic substrates in the presence of dimethylallyl diphosphate revealed the acceptance of all the tested tryptophan-containing cyclic dipeptides. structure elucidation of the main enzyme products by nmr and ms anal ... | 2015 | 25125042 |
| telomere-mediated chromosomal truncation in aspergillus oryzae. | we truncated the short arm of chromosome 3 to delete the aflatoxin biosynthesis gene homolog cluster using telomeric repeats in aspergillus oryzae. the predicted deletion was confirmed by southern blot analyses. this telomere-mediated chromosomal truncation method enables the development of an artificial chromosome in a. oryzae. | 2015 | 25034635 |
| supplementation with aspergillus oryzae-fermented kochujang lowers serum cholesterol in subjects with hyperlipidemia. | kochujang, a traditional fermented red pepper paste, is known for its hypocholesterolemic effect; however, these studies used non-commercial preparations of kochujang. in this study, we examined whether commercially-made kochujang in which aspergillus oryzae (also known as koji) was used as a microorganism for fermentation has the same cholesterol-lowering effects. | 2015 | 24961447 |
| microbial bioconversion and processing methods enhance the phenolic acid and flavonoids and the radical scavenging capacity of smilax china l. leaf. | it has been reported that smilax china l. leaf (scl) provided various biological functions owing to polyphenols. the objective of the current study was to assess the enhancing effect of processing methods and microbial conversions on phenolic acid and flavonoid content and radical scavenging capacity of scl for potential applications of diverse food products. | 2016 | 25754764 |
| aspergillus oryzae fermented germinated soybean extract alleviates perimenopausal symptoms in ovariectomised rats. | soybeans have been widely used to alleviate climacteric symptoms. in this study, we investigated the oestrogenic activities of isoflavones extracted from aspergillus oryzae-challenged germinated soybeans (ao-gs). eight-week-old virgin sprague-dawley female rats were ovariectomised (ovx). the rats were orally administered 0.1 mg kg(-1) 17α-ethinyl oestradiol or three different doses of ao-gs (0.5, 1.0 2.0 g kg(-1) day(-1)) in distilled water for 6 weeks, while control rats were administered vehic ... | 2016 | 25777819 |
| evaluation of kojic acid production in a repeated-batch pcs biofilm reactor. | in this study, kojic acid, a secondary metabolite as an industrially important compound, was produced by aspergillus oryzae (a. oryzae), which was immobilized in plastic composite support (pcs) bioreactor. nitrogen deficient medium was applied to increase the production of ka in pcs-immobilized bioreactor. the efficiency of immobilized culture for kojic acid (ka) production and the effect of morphology of a. oryzae on ka production were evaluated. after three cycles of cultivation, 83.47 g/l of ... | 2016 | 26657710 |
| enzymatically hydrolysed, acetylated and dually modified corn starch: physico-chemical, rheological and nutritional properties and effects on cake quality. | corn starch was treated by enzymatic hydrolysis with aspergillus oryzae s2 α-amylase, acetylation with vinyl acetate, and dual modification. the dual modified starch displayed a higher substitution degree than the acetylated starch and lower reducing sugar content than the hydrolysed starch. the results revealed that the cooling viscosity and amylose content of those products decrease (p < 0.05). an increase in moisture, water, and oil absorption capacity was observed for the acetylated starch a ... | 2016 | 26787967 |
| fermented brown rice extract causes apoptotic death of human acute lymphoblastic leukemia cells via death receptor pathway. | mixture of brown rice and rice bran fermented with aspergillus oryzae, designated as fbra, has been reported to reveal anti-carcinogenic and anti-inflammatory effects in rodents. then, to test its potential anti-cancer activity, the aqueous extract was prepared from fbra powder, and the effect of this extract on human acute lymphoblastic leukemia jurkat cells was directly examined. the exposure to fbra extract reduced the cell viability in a concentration- and time-dependent manner. the reductio ... | 2016 | 26769704 |
| occupational sensitization to lactase in the dietary supplement industry. | aerogen lactase exposure carries a risk for the development of allergic asthma and rhinitis; only a few occupationally affected patients have been reported. the authors report the results of allergy testing with employees of a lactase tablets manufacturing plant. the survey involved 13 workers, including a questionnaire, spirometry, basophil activation test (bat), and skin prick tests (spts) with lactase and a panel of common aeroallergens. furthermore, lactase-specific immunoglobulin e (ige) an ... | 2016 | 26134755 |
| stereoselective behavior of the chiral herbicides diclofop-methyl and diclofop during the soy sauce brewing process. | chiral pesticides are now receiving more and more attention in the food-making process. this experiment studied the enantioselective behavior of diclofop-methyl (dm) and its main metabolite, diclofop (dc), during the soy sauce brewing process. two kinds of commonly used strains, aspergillus oryzae and saccharomyces rouxii, were investigated. however, they showed a different degradation ability to the enantiomers of dm and dc. it was observed that (-)-(s)-dm was degraded much faster than (+)-(r)- ... | 2016 | 26501920 |
| a biosensor based on gold nanoparticles stabilized in poly(allylamine hydrochloride) and decorated with laccase for determination of dopamine. | gold nanoparticles stabilized in poly(allylamine hydrochloride) (aunp-pah) were used as a support for the immobilization of the enzyme laccase obtained from genetically-modified microorganisms (aspergillus oryzae) and successfully applied in the development of a new biosensor for the determination of dopamine by square-wave voltammetry. the electrochemical characterization of the biosensor was performed by cyclic voltammetry and electrochemical impedance spectroscopy and indicated that the nanom ... | 2016 | 26566574 |
| a long natural-antisense rna is accumulated in the conidia of aspergillus oryzae. | analysis of expressed sequence tag libraries from various culture conditions revealed the existence of conidia-specific transcripts assembled to putative conidiation-specific reductase gene (csra) in aspergillus oryzae. however, the all transcripts were transcribed with opposite direction to the gene csra. the sequence analysis of the transcript revealed that the rna overlapped mrna of csra with 3'-end, and did not code protein longer than 60 amino acid residues. we designated the transcript con ... | 2016 | 26539735 |
| thermostabilization of aspergillus oryzae β-d-galactosidase. | the thermal stability of the aspergillus oryzae β-d-galactosidase (β-gal) was evaluated at 60 °c. the stability of β-gal was dependent on the ph, buffer, and additives. the β-gal exhibited its highest thermal stability in a 0.02 m phosphate buffer ph 6. among all the tested additives, galactose, a competitive inhibitor of β-gal, was the upmost thermal stabilizer. a 0.15 m galactose solution caused β-gal to retain a whole of 98.65% of its initial activity after incubation at 60 °c for 2 h. the se ... | 2016 | 26011544 |
| an unusual chimeric diterpene synthase from emericella variecolor and its functional conversion into a sesterterpene synthase by domain swapping. | di- and sesterterpene synthases produce c20 and c25 isoprenoid scaffolds from geranylgeranyl pyrophosphate (ggpp) and geranylfarnesyl pyrophosphate (gfpp), respectively. by genome mining of the fungus emericella variecolor, we identified a multitasking chimeric terpene synthase, evvs, which has terpene cyclase (tc) and prenyltransferase (pt) domains. heterologous gene expression in aspergillus oryzae led to the isolation of variediene (1), a novel tricyclic diterpene hydrocarbon. intriguingly, i ... | 2016 | 26546087 |
| reconstitution of biosynthetic machinery of fungal polyketides: unexpected oxidations of biosynthetic intermediates by expression host. | reconstitution of whole biosynthetic genes in aspergillus oryzae has successfully applied for total biosynthesis of various fungal natural products. heterologous production of fungal metabolites sometimes suffers unexpected side reactions by host enzymes. in the studies on fungal polyketides solanapyrone and cytochalasin, unexpected oxidations of terminal olefin of biosynthetic intermediates were found to give one and four by-products by host enzymes of the transformants harboring biosynthetic g ... | 2016 | 26539980 |
| nematicidal activity of kojic acid produced by aspergillus oryzae against meloidogyne incognita. | the fungal strain eml-dml3pna1 isolated from leaf of white dogwood (cornus alba l.) showed strong nematicidal activity with juvenile mortality of 87.6% at a concentration of 20% fermentation broth filtrate at 3 days after treatment. the active fungal strain was identified as aspergillus oryzae, which belongs to section flavi, based on the morphological characteristics and sequence analysis of the its rdna, calmodulin (cam), and β-tubulin (bena) genes. the strain reduced the ph value to 5.62 afte ... | 2016 | 27197670 |
| production of alpha-amylase from aspergillus oryzae for several industrial applications in a single step. | a one-step method as a strategy of alpha-amylase concentration and purification was developed in this work. this methodology requires the use of a very low concentration of biodegradable polyelectrolyte (eudragit(®) e-po) and represents a low cost, fast, easy to scale up and non-polluting technology. besides, this methodology allows recycling the polymer after precipitation. the formation of reversible soluble/insoluble complexes between alpha-amylase and the polymer eudragit(®) e-po was studied ... | 2016 | 27085017 |
| aberrant glycosylation in the left ventricle and plasma of rats with cardiac hypertrophy and heart failure. | targeted proteomics focusing on post-translational modifications, including glycosylation, is a useful strategy for discovering novel biomarkers. to apply this strategy effectively to cardiac hypertrophy and resultant heart failure, we aimed to characterize glycosylation profiles in the left ventricle and plasma of rats with cardiac hypertrophy. dahl salt-sensitive hypertensive rats, a model of hypertension-induced cardiac hypertrophy, were fed a high-salt (8% nacl) diet starting at 6 weeks. as ... | 2016 | 27281159 |
| development of a promoter shutoff system in aspergillus oryzae using a sorbitol-sensitive promoter. | promoter shutoff is a general method for analyzing essential genes, but in the fungus aspergillus oryzae, no tightly repressed promoters have been reported. to overcome the current limitations of conditional promoters, we examined sorbitol- and galactose-responsive genes using microarrays to identify regulatable genes with only minor physiological and genetic effects. we identified two sorbitol-induced genes (designated as sora and sorb), cloned their promoters, and built a regulated egfp and br ... | 2016 | 27280333 |
| sequence- and structure-based functional annotation and assessment of metabolic transporters in aspergillus oryzae: a representative case study. | aspergillus oryzae is widely used for the industrial production of enzymes. in a. oryzae metabolism, transporters appear to play crucial roles in controlling the flux of molecules for energy generation, nutrients delivery, and waste elimination in the cell. while the a. oryzae genome sequence is available, transporter annotation remains limited and thus the connectivity of metabolic networks is incomplete. in this study, we developed a metabolic annotation strategy to understand the relationship ... | 2016 | 27274991 |
| modeling lactose hydrolysis for efficiency and selectivity: toward the preservation of sialyloligosaccharides in bovine colostrum whey permeate. | enzymatic hydrolysis of lactose has been shown to improve the efficiency and selectivity of membrane-based separations toward the recovery of bioactive oligosaccharides. achieving maximum lactose hydrolysis requires intrinsic process optimization for each specific substrate, but the effects of those processing conditions on the target oligosaccharides are not well understood. response surface methodology was used to investigate the effects of ph (3.25-8.25), temperature (35-55°c), reaction time ... | 2016 | 27236766 |
| heterologous expression and characterization of a thermostable exo-β-d-glucosaminidase from aspergillus oryzae. | an exo-β-d-glucosaminidase (aorcsxa) from aspergillus oryzae fl402 was heterologously expressed and purified. the deduced amino acid sequence indicated that aorcsxa belonged to glycoside hydrolase family 2. aorcsxa digested colloid chitosan into glucosamine but not into chitosan oligosaccharides, demonstrating exo-β-d-glucosaminidase (csxa) activity. aorcsxa exhibited optimal activity at ph 5.5 and 50°c; however, the enzyme expressed in pichia pastoris (ppaorcsxa) showed much stronger thermostab ... | 2016 | 26597529 |
| multi-response optimisation of the extraction solvent system for phenolics and antioxidant activities from fermented soy flour using a simplex-centroid design. | a simplex-centroid design comprising three solvents (water, ethanol and methanol) was used to optimise the extraction mixture for phenolics and antioxidant activities from defatted soy flour fermented with monascus purpureus or aspergillus oryzae. total phenolics were more efficiently extracted using only water for both samples. the highest antioxidant activities by the dpph and abts methods were obtained using extraction mixtures containing at least 75 wt% water. specific water:ethanol:methanol ... | 2016 | 26616938 |
| novel 4-methyl-2-oxopentanoate reductase involved in synthesis of the japanese sake flavor, ethyl leucate. | ethyl-2-hydroxy-4-methylpentanoate (ethyl leucate) contributes to a fruity flavor in japanese sake. the mold aspergillus oryzae synthesizes leucate from leucine and then the yeast saccharomyces cerevisiae produces ethyl leucate from leucate during sake fermentation. here, we investigated the enzyme involved in leucate synthesis by a. oryzae. the a. oryzae gene/cdna encoding the enzyme involved in leucate synthesis was identified and expressed in e. coli and a. oryzae host cells. the purified rec ... | 2016 | 26615399 |
| recombinant expression, purification, and characterization of an acyl-coa binding protein from aspergillus oryzae. | to characterize biochemically the lipid metabolism-regulating acyl-coa binding protein (acbp) from the industrially-important fungus aspergillus oryzae. | 2016 | 26677124 |
| potential pitfalls associated with testing of enzyme preparations in the salmonella/microsome assay. | the effect of a sample of food enzyme preparations on s9 activity was evaluated in bacterial mutation assays with the salmonella typhimurium strains ta98 and ta100 using benzo(a)pyrene, 2-aminoanthracene and 2-aminofluorene as model compounds. under the experimental conditions applied, aspergillus oryzae protease and porcine pancreas trypsin, applied at low non-toxic doses, proved to effectively inhibit the metabolic activation of benzo(a)pyrene by aroclor induced rat liver 9, while the activati ... | 2016 | 27330008 |
| six independent fucose-binding sites in the crystal structure of aspergillus oryzae lectin. | the crystal structure of aol (a fucose-specific lectin of aspergillus oryzae) has been solved by sad (single-wavelength anomalous diffraction) and mad (multi-wavelength anomalous diffraction) phasing of seleno-fucosides. the overall structure is a six-bladed β-propeller similar to that of other fucose-specific lectins. the fucose moieties of the seleno-fucosides are located in six fucose-binding sites. although the arg and glu/gln residues bound to the fucose moiety are common to all fucose-bind ... | 2016 | 27318092 |
| preventive effects of fermented brown rice and rice bran against prostate carcinogenesis in trap rats. | fermented brown rice and rice bran with aspergillus oryzae (fbra) is considered to have the potential to prevent chemically-induced carcinogenesis in multiple organs of rodents. in the present study, we evaluated the possible chemopreventive effects of fbra against prostate tumorigenesis. six-week-old male rats of the transgenic rat for adenocarcinoma of prostate (trap) strain were fed diets containing 5% or 10% fbra for 15 weeks. animals were sacrificed at 21 weeks of age, and the ventral and l ... | 2016 | 27409632 |
| comparison of catalytic properties of multiple β-glucosidases of trichoderma reesei. | ten putative trichoderma reesei β-glucosidase (bgl) isozymes were heterologously expressed in escherichia coli and aspergillus oryzae and purified to homogeneity. catalytic properties of nine enzymes which showed hydrolytic activity on cellobiose and p-nitrophenyl-β-d-glucopyranoside (pnpg) were investigated. three bgls, encoded by the genes cel3a, cel3b, and cel3e, contained a predicted signal peptide, showed higher hydrolytic activity on cello-oligosaccharides than on pnpg, and preferred longe ... | 2016 | 26846743 |
| antiviral and cytotoxic isocoumarin derivatives from an endophytic fungus aspergillus oryzae. | oryzaeins a-d (1-4), four new isocoumarin derivatives, along with five known ones (5-9) were isolated from solid cultures of an endophytic fungus aspergillus oryzae. their structures were elucidated by detailed spectroscopic analysis and by comparison with reported data of related derivatives. among them, compounds 1 and 2 represent the first examples of isocoumarins possessing an unusual 2-oxopropyl group and a rare 3-hydroxypropyl group. compounds 1 and 2 displayed moderate anti-tobacco mosaic ... | 2016 | 26824624 |
| anti-obesity effect of extract from fermented curcuma longa l. through regulation of adipogenesis and lipolysis pathway in high-fat diet-induced obese rats. | even though curcuma longa l. possesses various biological activities, it has strong flavor and taste, which decrease consumer palatability and limit industrial applications in food. | 2016 | 26822962 |
| comparative genomic analysis identified a mutation related to enhanced heterologous protein production in the filamentous fungus aspergillus oryzae. | genomic mapping of mutations using next-generation sequencing technologies has facilitated the identification of genes contributing to fundamental biological processes, including human diseases. however, few studies have used this approach to identify mutations contributing to heterologous protein production in industrial strains of filamentous fungi, such as aspergillus oryzae. in a screening of a. oryzae strains that hyper-produce human lysozyme (hly), we previously isolated an aut1 mutant tha ... | 2016 | 27405439 |
| identification and heterologous expression of the topopyrone nonaketide synthase gene from phoma sp. | non-reducing iterative type i polyketide synthase genes, pnk1 and pnk2, were cloned from the fungus phoma sp. baua2861, which produces the topoisomerase i inhibitors, topopyrones a to d. heterologous expression of these polyketide synthase genes under the α-amylase promoter in aspergillus oryzae was carried out to identify their functions. the pnk2 transformant produced topopyrones c, d, and haematommone. therefore, the pnk2 gene was found to encode for the topopyrone nonaketide synthase. | 2016 | 27373651 |
| structural characterization, catalytic, kinetic and thermodynamic properties of aspergillus oryzae tannase. | tannase (ec.3.1.1.20) from aspergillus oryzae was purified using ammonium sulphate precipitation (75%), gel filtration chromatography through sephadex g-100, and g-200. the purified enzyme was monomeric protein with a molecular mass of 106kda. the activation energy for tannic acid hydrolysis was 32.6kjmol(-1) and its temperature quotient (q10) was 1.0. the pka1 and pka2 values of acidic and basic limbs of the active site residues were 4.6 and 6.4. the calculated values of thermodynamic parameter ... | 2016 | 27373426 |
| chirally pure prodrugs and their converting enzymes lead to high supersaturation and rapid transcellular permeation of benzodiazepines. | water-soluble prodrugs can be rapidly converted by enzymes to hydrophobic drugs, whose aqueous thermodynamic solubilities are low, but are maintained in aqueous solution at supersaturated concentrations due to slow precipitation kinetics. recently, we investigated avizafone (avf) in combination with aspergillus oryzae protease as a prodrug/enzyme system intended to produce supersaturated diazepam (dzp). several fold enhancement of permeation of supersaturated dzp across madin-darby canine kidney ... | 2016 | 27342435 |
| identification of the gene encoding isoprimeverose-producing oligoxyloglucan hydrolase in aspergillus oryzae. | aspergillus oryzae produces a unique β-glucosidase, isoprimeverose-producing oligoxyloglucan hydrolase (ipase), that recognizes and releases isoprimeverose (α-d-xylopyranose-(1 → 6)-d-glucopyranose) units from the non-reducing ends of oligoxyloglucans. a gene encoding a. oryzae ipase, termed ipea, was identified and expressed in pichia pastoris. with the exception of cellobiose, ipea hydrolyzes a variety of oligoxyloglucans and is a member of the glycoside hydrolase family 3. xylopyranosyl branc ... | 2016 | 26755723 |
| fucosylation is associated with the malignant transformation of intraductal papillary mucinous neoplasms: a lectin microarray-based study. | intraductal papillary mucinous neoplasm (ipmn) is an intraductal mucin-producing pancreatic neoplasm with the potential for malignant transformation. changes in glycans expressed on the cell surface and glycotransferases play important roles in malignant transformation. we conducted this study to analyze glycan alterations in ipmns by using a lectin microarray and to identify the factors associated with altered glycans and their relationships with malignant transformation. | 2016 | 26754572 |
| illuminating the binding interactions of galactonoamidines during the inhibition of β-galactosidase (e. coli). | several galactonoamidines were previously identified as very potent competitive inhibitors that exhibit stabilizing hydrophobic interactions of the aglycon in the active site of β-galactosidase (aspergillus oryzae). to elucidate the contributions of the glycon to the overall inhibition ability of the compounds, three glyconoamidine derivatives with alteration in the glycon at c-2 and c-4 were synthesized and evaluated herein. all amidines are competitive inhibitors of β-galactosidase (escherichi ... | 2016 | 26740154 |
| production of 3-oxo-2-(2'-pentenyl)-cyclopentane-1-octanoic acid in the fungus aspergillus oryzae: a step towards heterologous production of pyrethrins in fungi. | pyrethrins are natural insecticides, which accumulate to high concentrations in pyrethrum (chrysanthemum cinerariaefolium) flowers. synthetic pyrethroids are more stable, more efficacious and cheaper, but contemporary requirements for safe and environmentally friendly pesticides encourage a return to the use of natural pyrethrins, and this would be favoured by development of an efficient route to their production by microbial fermentation. the biosynthesis of pyrethrins involves ester linkage be ... | 2016 | 26718544 |
| improving 3'-hydroxygenistein production in recombinant pichia pastoris using periodic hydrogen peroxide-shocking strategy. | 3'-hydroxygenistein can be obtained from the biotransformation of genistein by the engineered pichia pastoris x-33 strain, which harbors a fusion gene composed of cyp57b3 from aspergillus oryzae and a cytochrome p450 oxidoreductase gene (scpr) from saccharomyces cerevisiae. p. pastoris x-33 mutants with higher 3'-hydroxygenistein production were selected using a periodic hydrogen peroxide-shocking strategy. one mutant (p2-d14-5) produced 23.0 mg/l of 3'-hydroxygenistein, representing 1.87-fold m ... | 2016 | 26699751 |
| development of a genome editing technique using the crispr/cas9 system in the industrial filamentous fungus aspergillus oryzae. | to develop a genome editing method using the crispr/cas9 system in aspergillus oryzae, the industrial filamentous fungus used in japanese traditional fermentation and for the production of enzymes and heterologous proteins. | 2016 | 26687199 |
| metabolic engineering of long chain-polyunsaturated fatty acid biosynthetic pathway in oleaginous fungus for dihomo-gamma linolenic acid production. | microbial lipids are promising alternative sources of long chain-polyunsaturated fatty acids (lc-pufas) for food, feed, nutraceutical and pharmaceutical sectors. dihomo-γ-linolenic acid (c20:3δ(8,11,14); dgla) is an important lc-pufas with anti-inflammatory and anti-proliferative effects. to generate a dgla-producing strain, fatty acid reconstitution in aspergillus oryzae was performed by metabolic engineering through co-expression of codon-optimized pythium δ(6)-desaturase and δ(6)-elongase, wh ... | 2016 | 26686314 |
| chitosan crosslinked with genipin as support matrix for application in food process: support characterization and β-d-galactosidase immobilization. | in order to develop safer processes for the food industry, we prepared a chitosan support with the naturally occurring crosslinking reagent, genipin, for enzyme. as application model, it was tested for the immobilization of β-d-galactosidase from aspergillus oryzae. chitosan particles were obtained by precipitation followed by adsorption of the enzyme and crosslinking with genipin. the particles were characterized by fourier transform infrared (ftir) spectroscopy and thermogravimetric analysis ( ... | 2016 | 26686119 |
| further increased production of free fatty acids by overexpressing a predicted transketolase gene of the pentose phosphate pathway in aspergillus oryzae faaa disruptant. | free fatty acids are useful as source materials for the production of biodiesel fuel and various chemicals such as pharmaceuticals and dietary supplements. previously, we attained a 9.2-fold increase in free fatty acid productivity by disrupting a predicted acyl-coa synthetase gene (faaa, ao090011000642) in aspergillus oryzae. in this study, we achieved further increase in the productivity by overexpressing a predicted transketolase gene of the pentose phosphate pathway in the faaa disruptant. t ... | 2016 | 26998626 |
| astellifadiene: structure determination by nmr spectroscopy and crystalline sponge method, and elucidation of its biosynthesis. | genome mining of a terpene synthase gene from emericella variecolor nbrc 32302 and its functional expression in aspergillus oryzae led to the production of the new sesterterpene hydrocarbon, astellifadiene (1), having a 6-8-6-5-fused ring system. the structure of 1 was initially investigated by extensive nmr analyses, and was further confirmed by the crystalline sponge method, which established the absolute structure of 1 and demonstrated the usefulness of the method in the structure determinati ... | 2016 | 27038368 |
| enhancement of fructosyltransferase and fructooligosaccharides production by a. oryzae dia-mf in solid-state fermentation using aguamiel as culture medium. | the aim of this work was to improve the production of fructosyltransferase (ftase) by solid-state fermentation (ssf) using aguamiel (agave sap) as culture medium and aspergillus oryzae dia-mf as producer strain. ssf was carried out evaluating the following parameters: inoculum rate, incubation temperature, initial ph and packing density to determine the most significant factors through box-hunter and hunter design. the significant factors were then further optimized using a box-behnken design an ... | 2016 | 27036329 |
| efficient kinetic resolution of (rs)-1-phenylethanol by a mycelium-bound lipase from a wild-type aspergillus oryzae strain. | a mycelium-bound lipase from aspergillus oryzae (aol) exhibited excellent enantioselectivity for kinetic resolution of (rs)-1-phenylethanol in organic solvent. the various reaction parameters affecting the conversion and enantioselectivity were studied, including type of acyl donor, solvent, molar ratio, temperature, enzyme amount and substrate concentration. the optimum reaction conditions were found to be transesterification with vinyl acetate at 30°c in methyl tert-butyl ether with a vinyl ac ... | 2016 | 26854002 |
| optimization of liquid-state fermentation conditions for the glyphosate degradation enzyme production of strain aspergillus oryzae by ultraviolet mutagenesis. | this study aimed to obtain strains with high glyphosate-degrading ability and improve the ability of glyphosate degradation enzyme by the optimization of fermentation conditions. spore from aspergillus oryzae a-f02 was subjected to ultraviolet mutagenesis. single-factor experiment and response surface methodology were used to optimize glyphosate degradation enzyme production from mutant strain by liquid-state fermentation. four mutant strains were obtained and named as fujx 001, fujx 002, fujx 0 ... | 2016 | 26795747 |
| production of the natural iron chelator deferriferrichrysin from aspergillus oryzae and evaluation as a novel food-grade antioxidant. | deferriferrichrysin (dfcy) is a siderophore found in foods fermented by aspergillus oryzae and is a promising candidate for an antioxidant food additive because of its high binding constant toward iron. however, the dfcy concentration is typically low in foods and cultures. | 2016 | 26399367 |
| purification and enzymatic characterization of a novel β-1,6-glucosidase from aspergillus oryzae. | in this study, among the 10 genes that encode putative β-glucosidases in the glycoside hydrolase family 3 (gh3) with a signal peptide in the aspergillus oryzae genome, we found a novel gene (ao090038000425) encoding β-1,6-glucosidase with a substrate specificity for gentiobiose. the transformant harboring ao090038000425, which we named bglh, was overexpressed under the control of the improved glaa gene promoter to form a small clear zone around the colony in a plate assay using 4-methylumbellife ... | 2016 | 26320404 |
| molecular cloning and characterization of a novel bi-functional α-amylase/subtilisin inhibitor from hevea brasiliensis. | a novel cdna encoding a bi-functional α-amylase/subtilisin inhibitor (hbasi) was isolated from rubber (hevea brasiliensis) leaves cultivar rrim600. the hbasi had strong homology with the soybean trypsin inhibitor (kunitz) family of protease inhibitors. its putative amino acid sequence was similar to that of the α-amylase/subtilisin inhibitor from ricinus communis (72% identity). genomic sequencing indicated that the hbasi gene contained no introns. the messenger rna of hbasi was detected in leaf ... | 2016 | 26854410 |
| aorim15 is involved in conidial stress tolerance, conidiation and sclerotia formation in the filamentous fungus aspergillus oryzae. | the serine-threonine kinase rim15p is a master regulator of stress signaling and is required for stress tolerance and sexual sporulation in the yeast saccharomyces cerevisiae. however, in filamentous fungi that reproduce asexually via conidiation, the physiological function of rim15p homologs has not been extensively analyzed. here, we functionally characterized the protein homolog of rim15p in the filamentous fungus aspergillus oryzae, by deleting and overexpressing the corresponding aorim15 ge ... | 2016 | 26467693 |
| toward rational thermostabilization of aspergillus oryzae cutinase: insights into catalytic and structural stability. | cutinases are powerful hydrolases that can cleave ester bonds of polyesters such as poly(ethylene terephthalate) (pet), opening up new options for enzymatic routes for polymer recycling and surface modification reactions. cutinase from aspergillus oryzae (aoc) is promising owing to the presence of an extended groove near the catalytic triad which is important for the orientation of polymeric chains. however, the catalytic efficiency of aoc on rigid polymers like pet is limited by its low thermos ... | 2016 | 26522152 |
| biotransformation of l-tyrosine to dopamine by a calcium alginate immobilized mutant strain of aspergillus oryzae. | the present research work is concerned with the biotransformation of l-tyrosine to dopamine (da) by calcium alginate entrapped conidiospores of a mutant strain of aspergillus oryzae. different strains of a. oryzae were isolated from soil. out of 13 isolated strains, isolate-2 (i-2) was found to be a better da producer. the wild-type i-2 was chemically improved by treating it with different concentrations of ethyl methyl sulfonate (ems). among seven mutant variants, ems-6 exhibiting maximal da ac ... | 2016 | 27068833 |
| a novel non-thermostable deuterolysin from aspergillus oryzae. | three putative deuterolysin (ec 3.4.24.29) genes (deua, deub, and deuc) were found in the aspergillus oryzae genome database ( http://www.bio.nite.go.jp/dogan/project/view/ao ). one of these genes, deua, was corresponding to npii gene, previously reported. deua and deub were overexpressed by recombinant a. oryzae and were purified. the degradation profiles against protein substrates of both enzymes were similar, but deub showed wider substrate specificity against peptidyl mca-substrates compared ... | 2016 | 27050120 |
| substantial decrease in cell wall α-1,3-glucan caused by disruption of the kexb gene encoding a subtilisin-like processing protease in aspergillus oryzae. | disruption of the kexb encoding a subtilisin-like processing protease in aspergillus oryzae (δkexb) leads to substantial morphological defects when the cells are grown on czapek-dox agar plates. we previously found that the disruption of kexb causes a constitutive activation of the cell wall integrity pathway. to understand how the disruption of the kexb affects cell wall organization and components, we analyzed the cell wall of δkexb grown on the plates. the results revealed that both total n-a ... | 2016 | 26980104 |
| the c2h2-type transcription factor, flbc, is involved in the transcriptional regulation of aspergillus oryzae glucoamylase and protease genes specifically expressed in solid-state culture. | aspergillus oryzae produces a large amount of secreted proteins in solid-state culture, and some proteins such as glucoamylase (glab) and acid protease (pepa) are specifically produced in solid-state culture, but rarely in submerged culture. from the disruption mutant library of a. oryzae transcriptional regulators, we successfully identified a disruption mutant showing an extremely low production level of glab but a normal level of α-amylase production. this strain was a disruption mutant of th ... | 2016 | 26960315 |
| performance of an ultrafiltration membrane bioreactor (uf-mbr) as a processing strategy for the synthesis of galacto-oligosaccharides at high substrate concentrations. | the performance of an ultrafiltration membrane bioreactor for galacto-oligosaccharides (gos) synthesis using high lactose concentrations (470 g/l) and β-galactosidase from aspergillus oryzae was assessed. tested processing variables were: transmembrane-pressure (pt), crossflow-velocity (cfv) and temperature. results showed that processing variables had significant effect on the yield, the enzyme productivity and the flux but did not on gos concentration and reaction conversion obtained. as expec ... | 2016 | 26924238 |
| comparison of the effects of dietary single and multi-probiotics on growth, non-specific immune responses and disease resistance in starry flounder, platichthys stellatus. | an 8-week feeding trial was conducted to evaluate the effects of dietary probiotics on growth performance and non-specific immune responses in starry flounder, platichthys stellatus. fish averaging 46.5 ± 0.65 g (mean ± sd) were fed one of the six experimental diets; one control (cont), and five other diets were prepared by supplementing single-probiotics 1 (bacillus subtilis; sp1, 2 × 10(9) cfu kg(-1) diet), single-probiotics 2 (bacillus licheniformis; sp2, 2 × 10(9) cfu kg(-1) diet), multi-pro ... | 2016 | 27818339 |
| aflatoxin b1 detoxification by aspergillus oryzae from meju, a traditional korean fermented soybean starter. | aflatoxins are classified as group 1 (carcinogenic to humans) by the international agency for research on cancer (iarc). in this study, a total of 134 fungal strains were isolated from 65 meju samples, and two fungal isolates were selected as potential aflatoxin b₁ (afb₁)-biodetoxification fungi. these fungi were identified as aspergillus oryzae mao103 and a. oryzae mao104 by sequencing the beta-tubulin gene. the two a. oryzae strains were able to degrade more than 90% of afb1 (initial concentra ... | 2016 | 27817189 |
| functional characterization of cyp52g3 from aspergillus oryzae and its application for bioconversion and synthesis of hydroxyl flavanone and steroids. | aspergillus oryzae is a fungus widely used in traditional japanese fermentation industries. cytochrome p450 (cyp) proteins are ubiquitously distributed in nature and display a broad range of enzymatic activities. a novel cyp52 (cyp52g3) gene was found in a. oryzae. in this study, we report the functional characterization of cyp52g3. the recombinant protein was expressed heterologously in escherichia coli, and its membrane fraction isolated. cyp52g3 showed activities for 7-ethoxycoumarin and α-na ... | 2016 | 27028566 |
| valorization of by-products from palm oil mills for the production of generic fermentation media for microbial oil synthesis. | this study demonstrates the production of a generic nutrient-rich feedstock using by-product streams from palm oil production that could be used as a substitute for commercial fermentation supplements. solid-state fermentations of palm kernel cake (pkc) and palm-pressed fiber (ppf) were conducted in tray bioreactors and a rotating drum bioreactor by the fungal strain aspergillus oryzae for the production of crude enzymes. the production of protease was optimized (319.3 u/g) at an initial moistur ... | 2016 | 27787766 |
| a glycoprotein α-amylase inhibitor from withania somnifera differentially inhibits various α-amylases and affects the growth and development of tribolium castaneum. | identification and characterisation of plant defensive molecules enrich our resources to design crop protection strategies. in particular, plant-derived proteinaceous inhibitor(s) of insect digestive enzymes appear to be a safe, sustainable and attractive option. | 2016 | 27770482 |
| mitochondria-localized phospholipase a2, aoplaa, in aspergillus oryzae displays phosphatidylethanolamine-specific activity and is involved in the maintenance of mitochondrial phospholipid composition. | in mammals, cytosolic phospholipases a2 (cpla2s) play important physiological roles by releasing arachidonic acid, a precursor for bioactive lipid mediators, from the biological membranes. in contrast, fungal cpla2-like proteins are much less characterized and their roles have remained elusive. aoplaa is a cpla2-like protein in the filamentous fungus aspergillus oryzae which, unlike mammalian cpla2, localizes to mitochondria. in this study, we investigated the biochemical and physiological funct ... | 2016 | 27634187 |
| a set of isogenic auxotrophic strains for constructing multiple gene deletion mutants and parasexual crossings in aspergillus niger. | to construct a set of isogenic auxotrophic strains in aspergillus niger suited for creating multiple gene deletion mutants and executing parasexual crossings, we have combined mutations in genes involved in colour pigmentation (fwna and olva) with well-selectable auxotrophic markers (pyrg, nicb, argb, and adea). all markers, except for the pyrg marker, were introduced by targeted deletion, omitting uv mutagenesis of the strains. aspergillus oryzae orthologous genes of the argb, nicb, and adea ma ... | 2016 | 27251039 |
| nutrient assessment of olive leaf residues processed by solid-state fermentation as an innovative feedstuff additive. | olive leaf residue feedstuff additives were prepared by solid-state fermentation (ssf), and its feeding effects on broiler chickens were examined. | 2016 | 26991541 |
| genome mining and motif truncation of glycoside hydrolase family 5 endo-β-1,4-mannanase encoded by aspergillus oryzae rib40 for potential konjac flour hydrolysis or feed additive. | two novel glycosyl hydrolase family 5 (gh5) β-mannanases (aoman5a and aoman5b) were identified from aspergillus oryzae rib40 by genome mining. the aoman5a contains a predicted family 1 carbohydrate binding module (cbm-1), located at its n-terminal. the aoman5a, aoman5b and truncated mutant aoman5aδcl (truncating the n-terminal cbm and linker of aoman5a) were expressed retaining the n-terminus of the native protein in pichia pastoris gs115 by ppic9k(m). the specific enzyme activity of the purifie ... | 2016 | 27702490 |
| aspergillus oryzae-saccharomyces cerevisiae consortium allows bio-hybrid fuel cell to run on complex carbohydrates. | consortia of aspergillus oryzae and saccharomyces cerevisiae are examined for their abilities to turn complex carbohydrates into ethanol. to understand the interactions between microorganisms in consortia, fourier-transform infrared spectroscopy is used to follow the concentrations of various metabolites such as sugars (e.g., glucose, maltose), longer chain carbohydrates, and ethanol to optimize consortia conditions for the production of ethanol. it is shown that with proper design a. oryzae can ... | 2016 | 27681904 |
| valorization of sugar-to-ethanol process waste vinasse: a novel biorefinery approach using edible ascomycetes filamentous fungi. | the aim of the present work was to study the integration of edible ascomycetes filamentous fungi into the existing sugar- or molasses-to-ethanol processes, to grow on vinasse or stillage and produce ethanol and protein-rich fungal biomass. two fungal strains, neurospora intermedia and aspergillus oryzae were examined in shake flasks and airlift-bioreactors, resulting in reduction of vinasse cod by 34% and viscosity by 21%. utilization of glycerol and sugars were observed, yielding 202.4 or 222.8 ... | 2016 | 27668880 |
| biosynthesis of shearinine: diversification of a tandem prenyl moiety of fungal indole diterpenes. | the late-stage biosynthetic pathway of the indole diterpene shearinine involving four enzymatic reactions (janqdoj) was elucidated by an efficient heterologous expression system using aspergillus oryzae. key oxidative cyclization, forming a characteristic a/b bicyclic shearinine core by flavoprotein oxidase, was studied using a substrate analogue and a buffer containing h2(18)o. these experimental data provided evidence that jano catalyzes two-step oxidation via a hydroxylated product and that t ... | 2016 | 27632559 |
| identification of a novel sesquiterpene biosynthetic machinery involved in astellolide biosynthesis. | esterified drimane-type sesquiterpene lactones such as astellolides display various biological activities and are widely produced by plants and fungi. given their low homology to known sesquiterpene cyclases, the genes responsible for their biosynthesis have not been uncovered yet. here, we identified the astellolide gene cluster from aspergillus oryzae and discovered a novel sesquiterpene biosynthetic machinery consisting of astc, asti, and astk. all these enzymes are annotated as haloacid deha ... | 2016 | 27628599 |
| optimized expression of prolyl aminopeptidase in pichia pastoris and its characteristics after glycosylation. | prolyl aminopeptidases are specific exopeptidases that catalyze the hydrolysis of the n-terminus proline residue of peptides and proteins. in the present study, the prolyl aminopeptidase gene (pap) from aspergillus oryzae jn-412 was optimized through the codon usage of pichia pastoris. both the native and optimized pap genes were inserted into the expression vector ppic9 k and were successfully expressed in p. pastoris. additionally, the activity of the intracellular enzyme expressed by the reco ... | 2016 | 27628336 |
| japanese traditional dietary fungus koji aspergillus oryzae functions as a prebiotic for blautia coccoides through glycosylceramide: japanese dietary fungus koji is a new prebiotic. | the japanese traditional cuisine, washoku, considered to be responsible for increased longevity among the japanese, comprises various foods fermented with the non-pathogenic fungus aspergillus oryzae (koji). we have recently revealed that koji contains an abundant amount of glycosylceramide. intestinal microbes have significant effect on health. however, the effects of koji glycosylceramide on intestinal microbes have not been studied. | 2016 | 27563516 |
| evaluation of commercial soy sauce koji strains of aspergillus oryzae for γ-aminobutyric acid (gaba) production. | in this study, four selected commercial strains of aspergillus oryzae were collected from soy sauce koji. these a. oryzae strains designated as nsk, nsz, nsj and nst shared similar morphological characteristics with the reference strain (a. oryzae frr 1675) which confirmed them as a. oryzae species. they were further evaluated for their ability to produce γ-aminobutyric acid (gaba) by cultivating the spore suspension in a broth medium containing 0.4 % (w/v) of glutamic acid as a substrate for ga ... | 2016 | 27541157 |
| overproduction, purification and characterization of adenylate deaminase from aspergillus oryzae. | adenylate deaminase (ampd, ec 3.5.4.6) is an aminohydrolase that widely used in the food and medicine industries. in this study, the gene encoding aspergillus oryzae ampd was cloned and expressed in escherichia coli. induction with 0.75 mm isopropyl β-d-l-thiogalactopyranoside resulted in an enzyme activity of 1773.9 u/ml. recombinant ampd was purified to electrophoretic homogeneity using nickel affinity chromatography, and its molecular weight was calculated as 78.6 kda. purified ampd exhibited ... | 2016 | 27539573 |
| expression, purification and identification of a thermolysin-like protease, neutral protease i, from aspergillus oryzae with the pichia pastoris expression system. | neutral proteases are widely used in the textile, food and medical industries. this study was designed to obtain high expression levels of neutral protease i from aspergillus oryzae 3.042 by using pichia pastoris gs115 as the host strain for industrial purposes. the coding sequence of the target gene was modified, synthesized, and then cloned into the expression vector phbm905bdm, which harbored the d1+2 × 201 aox1 promoter and the mf4i leader sequence. the recombinant plasmid was transformed in ... | 2016 | 27539551 |
| erratum to: japanese traditional dietary fungus koji aspergillus oryzae functions as a prebiotic for blautia coccoides through glycosylceramide: japanese dietary fungus koji is a new prebiotic. | [this corrects the article doi: 10.1186/s40064-016-2950-6.]. | 2016 | 27822443 |