Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| identification of csypyrone b1 as the novel product of aspergillus oryzae type iii polyketide synthase csyb. | as a novel superfamily of type iii polyketide synthases (pkss) in microbes, four genes, csya, csyb, csyc, and csyd, were found in the genome of aspergillus oryzae, an industrially important filamentous fungus. although orthologs of csya, csyc, and csyd genes are present in a closely related species, aspergillus flavus, csyb gene is unique to a. oryzae. to identify its function, we carried out overexpression of csyb gene under the control of alpha-amylase promoter in a. oryzae. 3-(3-acetyl-4-hydr ... | 2010 | 20471846 |
| 4-deoxy-substrates for beta-n-acetylhexosaminidases: how to make use of their loose specificity. | beta-n-acetylhexosaminidases feature so-called wobbling specificity, which means that they cleave substrates both in gluco- and galacto- configurations, with the activity ratio depending on the enzyme source. here we present the new finding that fungal beta-n-acetylhexosaminidases are able to hydrolyze and transfer 4-deoxy-n-acetylhexosaminides with high yields. this clearly demonstrates that the 4-hydroxy moiety at the substrate pyranose ring is not essential for substrate binding to the enzyme ... | 2010 | 20466648 |
| use of the aspergillus oryzae actin gene promoter in a novel reporter system for exploring antifungal compounds and their target genes. | demand for novel antifungal drugs for medical and agricultural uses has been increasing because of the diversity of pathogenic fungi and the emergence of drug-resistant strains. genomic resources for various living species, including pathogenic fungi, can be utilized to develop novel and effective antifungal compounds. we used aspergillus oryzae as a model to construct a reporter system for exploring novel antifungal compounds and their target genes. the comprehensive gene expression analysis sh ... | 2010 | 20464390 |
| molecular cloning of ocpo encoding carboxypeptidase o of aspergillus oryzae iam2640. | carboxypeptidase o from aspergillus oryzae iam2640 is a serine-type carboxypeptidase. in this study, we cloned and sequenced cdna and genomic dna carrying ocpo encoding carboxypeptidase o. the results showed that the length of ocpo was 1,816 bp, and the open reading frame encoded a putative preproenzyme composed of 472 amino acid residues of the mature carboxypeptidase o and an additional n-terminal sequence of 50 amino acid residues. a blastn search revealed that a gene, ao090020000351, in a. o ... | 2010 | 20460731 |
| a eukaryotic copper-containing nitrite reductase derived from a nirk homolog gene of aspergillus oryzae. | we cloned a bacterial copper-containing nitrite reductase (nirk) homolog gene of aspergillus oryzae (aonirk). alignment showed that amino acid residues crucial for copper binding are conserved in the deduced sequence of the fungal protein. the recombinant protein exhibited distinct nitrite reductase activity, and its absorption and epr spectra showed the presence of type 1 and type 2 copper atoms in the molecule. aonirk transcriptionally responded to denitrification conditions. although the deni ... | 2010 | 20460712 |
| functional hypothesis on miraculin' sweetness by a molecular dynamics approach. | miraculin differs from other sweet-tasting proteins because it is a taste-modifier having the unusual property of modifying sourness into sweetness. its dimer is covalently linked by an inter-chain disulphide bond, and shows its taste-modifying activity at acidic ph, with maximum at ph 3.0, while it is flat at neutral ph. previous studies suggested the importance of two histidine residues for the taste-modifying activity of miraculin. in this work, we have conducted molecular dynamics simulation ... | 2010 | 20451498 |
| high-level expression and efficient purification of bioactive swollenin in aspergillus oryzae. | the bioactivity of swollenin is beneficial to cellulose decomposition by cellulase despite the lack of hydrolytic activity itself. in order to improve the productivity of swollenin, the effects of culture conditions on the expression level in recombinant aspergillus oryzae were investigated systematically. with regard to the bioactivity of swollenin, glycerin and peanut meal were the optimal carbon or nitrogen source, respectively. the highest level production of swollenin (50 mg l(-1)) was atta ... | 2010 | 20446057 |
| direct microbial conversion of wheat straw into lipid by a cellulolytic fungus of aspergillus oryzae a-4 in solid-state fermentation. | direct microbial conversion of wheat straw into lipid by a cellulolytic fungus of aspergillus oryzae a-4 in solid-state fermentation (ssf) was investigated. in submerged fermentation, a. oryzae a-4 accumulated lipid to 15-18.15% of biomass when pure cellulose was utilized as the sole substrate. in ssf of the wheat straw and bran mixture, a. oryzae a-4 yielded lipid of 36.6mg/g dry substrate (gds), and a cellulase activity of 1.82 fpu/gds with 25.25% of holocellulose utilization in the substrates ... | 2010 | 20444596 |
| microbiota during fermentation of chum salmon (oncorhynchus keta) sauce mash inoculated with halotolerant microbial starters: analyses using the plate count method and pcr-denaturing gradient gel electrophoresis (dgge). | nine different combinations of mugi koji (barley steamed and molded with aspergillus oryzae) and halotolerant microorganisms (htms), zygosaccharomyces rouxii, candida versatilis, and tetragenococcus halophilus, were inoculated into chum salmon sauce mash under a non-aseptic condition used in industrial fish sauce production and fermented at 35 +/- 2.5 degrees c for 84 days to elucidate the microbial dynamics (i.e., microbial count and microbiota) during fermentation. the viable count of halotole ... | 2010 | 20417400 |
| application of exogenous enzymes to beef muscle of high and low-connective tissue. | exogenous enzymes tenderize meat through proteolysis. triceps brachii and supraspinatus were randomly assigned to the seven enzyme treatments, papain, ficin, bromelain, homogenized fresh ginger, bacillus subtilis protease, and two aspergillus oryzae proteases or control to determine the extent of tenderization (warner-bratzler shear and sensory evaluation) and mode of action (myofibrillar or collagen degradation). sensory evaluation showed improvement (p<0.0009) for tenderness and connective tis ... | 2010 | 20416788 |
| effect of halotolerant starter microorganisms on chemical characteristics of fermented chum salmon (oncorhynchus keta) sauce. | chum salmon sauce mash was inoculated with barley koji (barley steamed and molded with aspergillus oryzae ) and halotolerant microorganisms (htms), zygosaccharomyces rouxii , candida versatilis , and tetragenococcus halophilus , in nine different combinations under non-aseptic conditions similar to the industrial fish sauce production and fermented at 35 +/- 2.5 degrees c for 84 days. the changes in the chemical components, color, and sensory properties during fermentation were investigated. fre ... | 2010 | 20405947 |
| survey of the transcriptome of aspergillus oryzae via massively parallel mrna sequencing. | aspergillus oryzae, an important filamentous fungus used in food fermentation and the enzyme industry, has been shown through genome sequencing and various other tools to have prominent features in its genomic composition. however, the functional complexity of the a. oryzae transcriptome has not yet been fully elucidated. here, we applied direct high-throughput paired-end rna-sequencing (rna-seq) to the transcriptome of a. oryzae under four different culture conditions. with the high resolution ... | 2010 | 20392818 |
| molecular strategy for identification in aspergillus section flavi. | aspergillus flavus is one of the most common contaminants that produces aflatoxins in foodstuffs. it is also a human allergen and a pathogen of animals and plants. aspergillus flavus is included in the aspergillus section flavi that comprises 11 closely related species producing different profiles of secondary metabolites. a six-step strategy has been developed that allows identification of nine of the 11 species. first, three real-time pcr reactions allowed us to discriminate four groups within ... | 2010 | 20377644 |
| molecular characterization and isolation of cytochrome p450 genes from the filamentous fungus aspergillus oryzae. | we explored the molecular diversity of cytochrome p450 genes in the filamentous fungus aspergillus oryzae using bioinformatic and experimental approaches. based on bioinformatic annotation, we found 155 putative genes of cytochromes p450 in the whole genome sequence; however, 13 of 155 appeared to be pseudogenes due to sequence deletions and/or inframe stop codon(s). there are 87 families of a. oryzae cytochromes p450 (aocyps), indicating considerable phylogenetic diversity. to characterize a. o ... | 2010 | 20358180 |
| analysis of genome-wide coexpression and coevolution of aspergillus oryzae and aspergillus niger. | analysis of coexpressed genes in response to different perturbations at the genome-level can provide new insight into global regulatory structures. here we performed integrated data analysis for a crossspecies comparative investigation by exploring genomes and transcriptional coexpression profiles in aspergillus oryzae and aspergillus niger. based on our analysis of conserved coexpressed genes, fatty acid catabolism via beta-oxidation, fatty acid transport, the glyoxylate bypass, and peroxisomal ... | 2010 | 20337533 |
| development and refinement of a high-efficiency gene-targeting system for aspergillus flavus. | an efficient gene-targeting system based on impairment of the nonhomologous end-joining pathway and the orotidine monophosphate decarboxylase gene (pyrg) in aspergillus flavus was established. it was achieved by replacing the ku70 gene with the aspergillus oryzae pyrithiamine resistance (ptr) gene and by inserting the aspergillus parasiticus cypa gene into the pyrg locus. the utility of this system was demonstrated by disruption of nine candidate genes for conidial pigment biosynthesis. the gene ... | 2010 | 20298723 |
| the formation of kojic acid (3-hydroxy-6-hydroxymethyl-gamma pyrone) from ethyl alcohol by aspergillus oryzae. | 1946 | 20277276 | |
| a study of the action of purified amylase from aspergillus oryzae, taka-amylase. | 1947 | 20272106 | |
| mutation of key residues in the c-methyltransferase domain of a fungal highly reducing polyketide synthase. | site directed mutations of the c-methyltransferase domain of squalestatin tetraketide synthase were made in an attempt to alter the methylation pattern of the synthase expressed in vivo: mutation resulted in either no effect or in complete abrogation of polyketide production. | 2010 | 20237645 |
| stimulation of lignocellulosic biomass hydrolysis by proteins of glycoside hydrolase family 61: structure and function of a large, enigmatic family. | currently, the relatively high cost of enzymes such as glycoside hydrolases that catalyze cellulose hydrolysis represents a barrier to commercialization of a biorefinery capable of producing renewable transportable fuels such as ethanol from abundant lignocellulosic biomass. among the many families of glycoside hydrolases that catalyze cellulose and hemicellulose hydrolysis, few are more enigmatic than family 61 (gh61), originally classified based on measurement of very weak endo-1,4-beta-d-gluc ... | 2010 | 20230050 |
| some biochemical and physical changes during the preparation of the enzyme-ripening sufu, a fermented product of soybean curd. | in this study, sufu, a fermented product of soybean curd, was prepared by ripening the salted tofu in the mash of aspergillus oryzae -fermented rice-soybean koji possessing various hydrolytic enzymes for 16 days. it was found that protease, alpha-amylase, beta-amylase, and lipase activities observed in the koji granules reduced most pronouncedly during the initial 4 or 8 days of ripening. meanwhile, an increase in the activity of the various enzymes was noted in the ripening infusion and tofu cu ... | 2010 | 20218723 |
| aspergillus oryzae flavohemoglobins promote oxidative damage by hydrogen peroxide. | apart from their well-established role in nitric oxide detoxification, flavohemoglobins (fhbs) are also believed to be involved in protection against oxidative stress in some yeast and bacteria. however, different studies have reported contradictory results in this regard. here, we investigate the relationship between two fhbs in aspergillus oryzae (cytosolic fhb1 and mitochondrial fhb2) and oxidative stress. the strains deficient in the two fhbs exhibited higher resistance to hydrogen peroxide ... | 2010 | 20211603 |
| promotion of efficient saccharification of crystalline cellulose by aspergillus fumigatus swo1. | swollenin is a protein from trichoderma reesei that has a unique activity for disrupting cellulosic materials, and it has sequence similarity to expansins, plant cell wall proteins that have a loosening effect that leads to cell wall enlargement. in this study we cloned a gene encoding a swollenin-like protein, swo1, from the filamentous fungus aspergillus fumigatus, and designated the gene afswo1. afswo1 has a bimodular structure composed of a carbohydrate-binding module family 1 (cbm1) domain ... | 2010 | 20173066 |
| characterization of water-soluble complexes of polyacrylic acid with alpha-amylase from aspergillus oryzae. | polycomplex formation of alpha-amylase from aspergillus oryzae (taka) with polyacrylic acid (paa) was studied by ph titration, fluorescence, and high performance liquid chromatography (hplc) methods in water solutions. according to the our results, the complex formation and its solubility were depended on nature of enzyme and the ph of solutions. both of them correlates isoelectric points (pi). the stability of paa-amylase complexes was negligibly weak at ph 7 [ph > pi (isoelectric ph)]. stable ... | 2010 | 20165908 |
| what does genetic diversity of aspergillus flavus tell us about aspergillus oryzae? | aspergillus flavus and aspergillus oryzae belong to aspergillus section flavi. they are closely related and are of significant economic importance. the former species has the ability to produce harmful aflatoxins while the latter is widely used in food fermentation and industrial enzyme production. this review summarizes the current understanding of the similarity of the a. flavus and a. oryzae genomes, the genetic diversity in a. flavus and a. oryzae populations, the causes of this diversity, a ... | 2010 | 20163884 |
| cultivation characteristics and gene expression profiles of aspergillus oryzae by membrane-surface liquid culture, shaking-flask culture, and agar-plate culture. | we cultivated a filamentous fungus, aspergillus oryzae iam 2706 by three different cultivation methods, i.e., shaking-flask culture (sfc), agar-plate culture (apc), and membrane-surface liquid culture (mslc), to elucidate the differences of its behaviors by different cultivation methods under the same media, by measuring the growth, secretion of proteases and alpha-amylase, secreted protein level, and gene transcriptional profile by the dna microarray analysis. the protease activities detected b ... | 2010 | 20159576 |
| production of xylanase with a transformant of aspergillus oryzae rib40 in a liquid-surface immobilization (lsi) system. | xylanase production by a xynf1 (33 kda)-transformant of aspergillus oryzae rib40 was compared between submerged cultivation (smc) and liquid-surface immobilization (lsi) systems. while the accumulation of xylanase in the smc decreased by prolonged incubation, lsi system enabled the continuation of xylanase production to afford 4.5-fold xylanase production compared with the smc system. | 2010 | 20159567 |
| analysis of wide-domain transcriptional regulation in solid-state cultures of aspergillus oryzae. | many filamentous fungi secrete considerable quantities of enzymes including protease, cellulase and xylanase, which are of major industrial importance. over the past few decades, many of these fungal enzymes have been isolated and their relevant genes characterised. solid-state fermentation (ssf), an ancient technique described as a fermentation process performed on non-soluble material whereby the material acts as a physical support and as a source of nutrients, is widely employed in the produc ... | 2010 | 20145973 |
| characterization of aspergillus oryzae glycoside hydrolase family 43 beta-xylosidase expressed in escherichia coli. | this is the first report of glycoside hydrolase family 43 beta-xylosidase from aspergillus oryzae. to characterize this enzyme, the recombinant enzyme was expressed in escherichia coli. unlike known beta-xylosidases from fungal origins, the enzyme did not show substrate ambiguity and was stable at alkaline ph. | 2010 | 20129093 |
| l-ascorbate, a strong inducer of l-dopa (3,4-dihydroxy-l-phenylalanine) production from pre-grown mycelia of aspergillus oryzae nrrl-1560. | the inductive effect of l-ascorbate on the microbiological production of l-dopa (3,4-dihydroxy-l-phenylalanine) from aspergillus oryzae nrrl-1560 was investigated. all biochemical reactions were performed aerobically using mould mycelia as a source of enzyme tyrosinase and acetate buffer (ph 3.0) as an extractant. l-tyrosine as a substrate was added at a level of 2.5 mg/ml. maximal l-dopa production (1.876 mg/ml) was achieved when l-ascorbate (5.0 mg/ml) was added 6 min after the initiation of t ... | 2010 | 20121699 |
| molecular strategy for identification in aspergillus section flavi. | abstract aspergillus flavus is one of the most common contaminants that produces aflatoxins in foodstuffs. it is also a human allergen and a pathogen of animals and plants. aspergillus flavus is included in the aspergillus section flavi that comprises 11 closely related species producing different profiles of secondary metabolites. a six-step strategy has been developed that allows identification of nine of the 11 species. first, three real-time pcr reactions allowed us to discriminate four grou ... | 2010 | 20113354 |
| molecular cloning, characterization and expression analysis of two beta-n-acetylhexosaminidase homologs of coccidioides posadasii. | two full-length cdnas were isolated from coccidioides posadasii that encode two deduced proteins (cphex1 and cphex2) with homology to the glycosyl hydrolase 20 family of beta-n-acetylhexosaminidases. cphex1 consists of 595 amino acids, has a predicted molecular mass of 68 kda and shares the highest identity with the n-acetylhexosaminidase (naga) of aspergillus nidulans, while cphex2 consists of 603 amino acids, has a predicted molecular mass of 68.5 kda and shares the highest identity with nag1 ... | 2010 | 20109094 |
| enhanced thermal and ultrasonic stability of a fungal protease encapsulated within biomimetically generated silicate nanospheres. | dendrimers are highly branched synthetic macromolecules with a globular shape. they have been successfully used for generation of nanospheres at mild conditions via biomimetic silicification. encapsulation of enzyme molecules within these nanospheres during their synthesis is a promising method for rapid and efficient entrapment of several enzymes. however, encapsulation of proteolytic enzymes has been rarely done via biomimetic silicification. as well, the operational stability of encapsulated ... | 2010 | 20100547 |
| high-level expression of lactobacillus beta-galactosidases in lactococcus lactis using the food-grade, nisin-controlled expression system nice. | in this work the overlapping genes (lacl and lacm) encoding heterodimeric beta-galactosidases from lactobacillus reuteri , lb. acidophilus , lb. sakei , and lb. plantarum were cloned into two different nisin-controlled expression (nice) vectors and expressed using lactococcus lactis nz9000 and nz3900 as hosts. the lacl gene, encoding the large subunit of the beta-galactosidases, was fused translationally downstream of the nisin-inducible promoter nisa. chloramphenicol was employed as selection m ... | 2010 | 20092320 |
| characterization of aroma compounds in chinese rice wine qu by solvent-assisted flavor evaporation and headspace solid-phase microextraction. | the aroma of chinese rice wine qu is one of the most important factors that influences the flavor of chinese rice wine. to better understand the aroma of qu, aroma compounds in four wheat qus and two xiao qus were identified by chromatography-olfactometry (gc-o) after solvent extraction followed by solvent-assisted flavor evaporation (safe). a total of 39 aroma compounds were characterized by gc-o. on the basis of aroma intensity, 1-hexanal, ethyl hexanoate, 1-octen-3-ol, and phenylacetaldehyde ... | 2010 | 20088505 |
| evaluation of enzyme mixtures in releasing fermentable sugars from pre-pulping extracts of mixed northeast hardwoods. | one near-term option to developing a forest product biorefinery is to derive pre-pulping extract from incoming wood chips before the main pulping step. the release of monomer sugars from a xylan-rich extract, creating a fermentable substrate is a prerequisite for utilization of pre-pulping extract for production of ethanol or other value-added products. this study examined the individual and mixture efficiencies of two hemicellulolytic microbial enzymes and two xylanase preparations in catalyzin ... | 2010 | 20084471 |
| characterization of the palh gene from aspergillus oryzae. | we disrupted the palh gene, which is known to participate in the ambient ph signal transduction pathway, in aspergillus oryzae. palh disruption caused significant decreases in pacc expression and alkaline protease activity. hence we believe that palh plays a very important role in controlling the alkaline protease level in a. oryzae. | 2010 | 20057127 |
| bioreduction of trivalent aurum to nano-crystalline gold particles by active and inactive cells and cell-free extract of aspergillus oryzae var. viridis. | bioreduction efficacy of both active (ab) and inactive (ib) cells/biomass of aspergillus oryzae var. viridis and their respective cell-free extracts (ace and ice) to convert trivalent aurum to gold nanoparticles were tested in the present study. strong plasmon resonance of gold nanoparticles was observed between 540 and 560 nm in the samples obtained from ab, ib, ace and ice. transmission electron microscopy (tem), field emission scanning electron microscopy (fe-sem), energy dispersive x-ray (ed ... | 2010 | 20056324 |
| distinct enzymatic and cellular characteristics of two secretory phospholipases a2 in the filamentous fungus aspergillus oryzae. | microbial secretory phospholipases a(2) (spla(2)s) are among the last discovered and least known members of this functionally diverse family of enzymes. we analyzed here two spla(2)s, named splaa and splab, of the filamentous ascomycete aspergillus oryzae. splaa and splab consist of 222 and 160 amino acids, respectively, and share the conserved cys and catalytic his-asp residues typical of microbial spla(2)s. two spla(2)s differ in ph optimum, ca(2+) requirement and expression profile. the splaa ... | 2010 | 20045482 |
| characterization of recombinant prolyl aminopeptidase from aspergillus oryzae. | prolyl aminopeptidase (pap) degrades only amino-terminal proline from peptides. the food-grade fungus aspergillus oryzae produces this enzyme only in small amounts. in this paper, we present efficient production of recombinant pap with an overexpression system of a. oryzae and characterization of its biochemical properties. | 2010 | 20028436 |
| production of fructooligosaccharides by beta-fructofuranosidases from aspergillus oryzae kb. | aspergillus oryzae kb produces two types of beta-fructofuranosidases: f1 and f2. f1 produces the fructooligosaccharides (foss) 1-kestose, nystose, and fructosyl nystose from sucrose through a transfructosylation action, whereas f2 mainly hydrolyzes sucrose to glucose and fructose. f1 and f2 enzymes were more selectively produced from the kb strain in liquid media with a sucrose concentration>2% and <2%, respectively. immobilization using an anion-exchange resin (wa-30; polystyrene with tertiary ... | 2010 | 20014851 |
| enzymatic degradation products from a marine polysaccharide ycp with different immunological activity and binding affinity to macrophages, hydrolyzed by alpha-amylases from different origins. | ycp is a marine polysaccharide with anti-tumor and immune-modulating effects. this study evaluated the effect of enzymatic degradation of ycp by alpha-amylases from different origins on its immunological activity and binding ability to the macrophages. ycp was hydrolyzed by alpha-amylases isolated from aspergillus oryzae, bacillus licheniformis, barley malt, and porcine pancreas respectively, then four fragments with unique molecular weight (termed: ycp-ao, ycp-bl, ycp-bm, and ycp-pp, respective ... | 2010 | 20004229 |
| simultaneous preparation of purified plasmalogens and sphingomyelin in human erythrocytes with phospholipase a1 from aspergillus orizae. | a method for the simultaneous purification of plasmalogens and sphingomyelin (sm) in human erythrocytes is described. treatment of total lipids with n-hexane/acetone (1:1 v/v) resulted in selective precipitation of sm. both the supernatant and the precipitate fractions were incubated with a phospholipase a(1) (pla1) from aspergillus orizae for 3.5 h. the pla1-treated lipids were extracted with n-hexane/isopropanol, the hexane layer was obtained using a na(2)so(4) solution, and the hexane layer w ... | 2009 | 19966491 |
| expression in escherichia coli of an unnamed protein gene from aspergillus oryzae rib40 and cofactor analyses of the gene product as formate oxidase. | an unnamed protein of aspergillus oryzae rib40 (accession no. xp_001727378), the amino acid sequence of which shows high similarity to those of formate oxidase isoforms produced by debaryomyces vanjiriae mh201, was produced in escherichia coli in c-his(6)-tagged form. the gene product, purified by affinity column chromatography, catalyzed the oxidation of formate to yield hydrogen peroxide but showed no evidence of activity on the other substrates tested. the k(m) and v(max) values at 30 degrees ... | 2009 | 19966484 |
| hydrolysis of amylopectin by amylolytic enzymes: level of inner chain attack as an important analytical differentiation criterion. | differences in amylase action pattern on amylopectin were demonstrated by the relation between the decrease in potassium iodide-iodine binding of waxy maize starch and the increase in reducing value during hydrolysis, as expressed by the rv(80) value (i.e., the reducing value for a potassium iodide-iodine binding value of 80% of that of the starting material). in the initial stages of the hydrolysis, the ratio of the increase in the level of reducing polysaccharides to the increase in the total ... | 2010 | 19962134 |
| hydrolysis of amylopectin by amylolytic enzymes: structural analysis of the residual amylopectin population. | amylopectin fine structures were studied following limited hydrolysis of gelatinised waxy maize starch by amylases with a different level of inner chain attack (lica). this was done by size exclusion chromatography as well as by debranching the (partially hydrolysed) amylopectin samples and studying the size distributions of the released chains. alpha-amylases from bacillus amyloliquefaciens and aspergillus oryzae, with a relatively high lica, drastically altered amylopectin chain length distrib ... | 2010 | 19962130 |
| aoso protein accumulates at the septal pore in response to various stresses in the filamentous fungus aspergillus oryzae. | filamentous ascomycetes form hyphal networks that are compartmentalized by septa which have a perforated pore allowing the passage of cytoplasm and organelles between adjacent hyphal compartments. thus, the septal pore may play an important role in the organized growth of multicellular organisms. upon hyphal injury, the septal pore is plugged by a wound-healing organelle, known as the woronin body, to prevent excessive cytoplasmic leakage. however, the movement of proteins towards the septal por ... | 2010 | 19945422 |
| using a new gpi-anchored-protein identification system to mine the protein databases of aspergillus fumigatus, aspergillus nidulans, and aspergillus oryzae. | computational approaches provide valuable information to start experimental surveys identifying glycosylphosphatidylinositol (gpi)-anchored proteins in protein sequence databases. we developed a new sequence-based identification system that uses an optimized classifier based on a support vector machine (svm) algorithm to recognize appropriate cooh-terminal sequences and uses a classifier implementing a simple majority voting strategy to recognize appropriate nh2-terminal sequences. the svm class ... | 2009 | 19940384 |
| acid protease production by solid-state fermentation using aspergillus oryzae mtcc 5341: optimization of process parameters. | aspergillus oryzae mtcc 5341, when grown on wheat bran as substrate, produces several extracellular acid proteases. production of the major acid protease (constituting 34% of the total) by solid-state fermentation is optimized. optimum operating conditions obtained are determined as ph 5, temperature of incubation of 30 degrees c, defatted soy flour addition of 4%, and fermentation time of 120 h, resulting in acid protease production of 8.64 x 10(5) u/g bran. response-surface methodology is used ... | 2010 | 19937364 |
| analysis of bacterial and fungal communities in japanese- and chinese-fermented soybean pastes using nested pcr-dgge. | the microbial diversity of japanese- and chinese-fermented soybean pastes was investigated using nested pcr-denaturing gradient gel electrophoresis (dgge). five japanese-fermented soybean paste samples and three chinese-fermented soybean paste samples were analyzed for bacteria and fungi. extracted dna was used as a template for pcr to amplify 16s rrna and 18s rrna genes. the nearly complete 16s rrna and 18s rrna genes were amplified using universal primers, and the resulting products were subse ... | 2010 | 19924476 |
| improvement of heterologous protein production in aspergillus oryzae by rna interference with alpha-amylase genes. | aspergillus oryzae rib40 has three alpha-amylase genes (amya, amyb, and amyc), and secretes alpha-amylase abundantly. however, large amounts of endogenous secretory proteins such as alpha-amylase can compete with heterologous protein in the secretory pathway and decrease its production yields. in this study, we examined the effects of suppression of alpha-amylase on heterologous protein production in a. oryzae, using the bovine chymosin (chy) as a reporter heterologous protein. the three alpha-a ... | 2009 | 19897917 |
| characterization of galactosyl derivatives obtained by transgalactosylation of lactose and different polyols using immobilized beta-galactosidase from aspergillus oryzae. | the synthesis of novel galactosides is interesting because of their important role in several biological processes. their properties greatly depend upon the configuration and type of galactoside. therefore, to study biological activity, it is essential to elucidate the structure of the products. glycosidases are capable of catalyzing glycosidic linkages with absolute stereoselectivity of the anomeric center. we report the enzymatic synthesis of galactosyl-ethylene glycol, galactosyl-glycerol, an ... | 2009 | 19891502 |
| a cytosolic phospholipase a2-like protein in the filamentous fungus aspergillus oryzae localizes to the intramembrane space of the mitochondria. | in mammalian cells, cytosolic phospholipase a(2) (cpla(2)) displays a variety of activities through the release of arachidonic acid in response to cellular stimuli. in this study, we characterize the putative cpla(2)-like protein, aoplaa, in the filamentous fungus aspergillus oryzae. when aoplaa-egfp was expressed in a. oryzae, it localized to the tubular structures that was costained by the marker dye for the mitochondria. a biochemical fractionation experiment showed that aoplaa was present in ... | 2009 | 19889028 |
| characterization of cyclo-acetoacetyl-l-tryptophan dimethylallyltransferase in cyclopiazonic acid biosynthesis: substrate promiscuity and site directed mutagenesis studies. | the fungal neurotoxin alpha-cyclopiazonic acid (cpa), a nanomolar inhibitor of ca(2+)-atpase with a unique pentacyclic indole tetramic acid scaffold, is assembled by a three enzyme pathway cpas, cpad, and cpao in aspergillus sp. we recently characterized the first pathway-specific enzyme cpas, a hybrid two module polyketide synthase-nonribosomal peptide synthetase (pks-nrps) that generates cyclo-acetoacetyl-l-tryptophan (caatrp). here we report the characterization of the second pathway-specific ... | 2009 | 19877600 |
| a trial of minimization of chromosome 7 in aspergillus oryzae by multiple chromosomal deletions. | we aim to create an aspergillus oryzae mutant with a highly reduced chromosome, but better growth, by eliminating the nonessential regions coding various dispensable functions for its better industrial use. in our previous study, we successfully determined the outline of essential and nonessential regions by constructing a series of large chromosomal deletions in a. oryzae chromosome 7. based on these results, we here constructed two mutants, designated rkuaf7a and rkuaf7b, lacking 24.7 and 24% ... | 2010 | 19855999 |
| characterization and expression analysis of a maltose-utilizing (mal) cluster in aspergillus oryzae. | starch and maltooligosaccharides such as maltose and maltotriose induce the production of amylolytic enzymes including alpha-amylase in aspergillus oryzae. a transcriptional activator gene amyr, required for maltose induction of amylolytic enzymes, has been cloned and characterized. the amyr gene deletion mutant showed significantly poor growth on starch medium but normal growth on maltose medium. this indicated the existence of another maltose-utilizing system, whose expression might not be con ... | 2010 | 19850146 |
| genetic analysis of conidiation regulatory pathways in koji-mold aspergillus oryzae. | conidia of koji-mold aspergillus oryzae are often used as starters in the fermented food industry. however, little is known about conidiation regulation in a. oryzae. to improve the productivity of conidia in a. oryzae, it is necessary to understand conidiation regulation in the strain. therefore, we analyzed the conidiation regulatory system in a. oryzae using 10 kinds of conidiation regulatory gene disruptants. the phenotypes of aorflug, aorflba, aorflbb, aorflbc, aorflbd, aorflbe, aorbrla, ao ... | 2010 | 19850144 |
| biosensor for luteolin based on silver or gold nanoparticles in ionic liquid and laccase immobilized in chitosan modified with cyanuric chloride. | novel and effective biosensors based on ag or au nanoparticles dispersed in ionic liquid (il) 1-butyl-3-methylimidazolium hexafluorophosphate (bmi.pf(6)) and laccase (lac) from aspergillus oryzae immobilized in chitosan (chi) chemically cross-linked with cyanuric chloride (cc) were constructed. this enzyme catalyzes the oxidation of luteolin to the corresponding o-quinone, which is electrochemically reduced back to luteolin at 0.35 v vs. ag/agcl. square-wave voltammetry was used for the electroc ... | 2009 | 19838422 |
| structural and functional studies of aspergillus oryzae cutinase: enhanced thermostability and hydrolytic activity of synthetic ester and polyester degradation. | cutinases are responsible for hydrolysis of the protective cutin lipid polyester matrix in plants and thus have been exploited for hydrolysis of small molecule esters and polyesters. here we explore the reactivity, stability, and structure of aspergillus oryzae cutinase and compare it to the well-studied enzyme from fusarium solani. two critical differences are highlighted in the crystallographic analysis of the a. oryzae structure: (i) an additional disulfide bond and (ii) a topologically favor ... | 2009 | 19810726 |
| analysis of enzyme production by submerged culture of aspergillus oryzae using whole barley. | we have reported on high enzyme production by submerged culture of aspergillus kawachii using barley with the husk (whole barley). to elucidate the mechanism underlying this high enzyme production, we performed a detailed analysis. aspergillus oryzae rib40 was submerged-cultured using whole barley and milled whole barley. enzyme production was analyzed in terms of changes in medium components and gene expression levels. when whole barley was used, high production of glucoamylase and alpha-amylas ... | 2009 | 19809198 |
| acrylamide degradation by filamentous fungi used in food and beverage industries. | filamentous fungi (24 strains) used in food and beverage industries were investigated for acrylamide-degradation ability: aspergillus oryzae kbn1010 showed the highest ability. little acrylic acid was produced but no glycidamide was detected during aa degradation in roasted green tea; therefore, a. oryzae could be used for reducing the aa concentration. | 2009 | 19804862 |
| identification of two aflatrem biosynthesis gene loci in aspergillus flavus and metabolic engineering of penicillium paxilli to elucidate their function. | aflatrem is a potent tremorgenic toxin produced by the soil fungus aspergillus flavus, and a member of a structurally diverse group of fungal secondary metabolites known as indole-diterpenes. gene clusters for indole-diterpene biosynthesis have recently been described in several species of filamentous fungi. a search of aspergillus complete genome sequence data identified putative aflatrem gene clusters in the genomes of a. flavus and aspergillus oryzae. in both species the genes for aflatrem bi ... | 2009 | 19801473 |
| discovery of a new tyrosinase-like enzyme family lacking a c-terminally processed domain: production and characterization of an aspergillus oryzae catechol oxidase. | a homology search against public fungal genome sequences was performed to discover novel secreted tyrosinases. the analyzed proteins could be divided in two groups with different lengths (350-400 and 400-600 residues), suggesting the presence of a new class of secreted enzymes lacking the c-terminal domain. among them, a sequence from aspergillus oryzae (408 aa, aoco4) was selected for production and characterization. aoco4 was expressed in trichoderma reesei under the strong cbh1 promoter. expr ... | 2010 | 19798497 |
| uncovering transcriptional regulation of glycerol metabolism in aspergilli through genome-wide gene expression data analysis. | glycerol is catabolized by a wide range of microorganisms including aspergillus species. to identify the transcriptional regulation of glycerol metabolism in aspergillus, we analyzed data from triplicate batch fermentations of three different aspergilli (aspergillus nidulans, aspergillus oryzae and aspergillus niger) with glucose and glycerol as carbon sources. protein comparisons and cross-analysis with gene expression data of all three species resulted in the identification of 88 genes having ... | 2009 | 19784673 |
| genes regulated by aoxlnr, the xylanolytic and cellulolytic transcriptional regulator, in aspergillus oryzae. | xlnr is a zn(ii)2cys6 transcriptional activator of xylanolytic and cellulolytic genes in aspergillus. overexpression of the aoxlnr gene in aspergillus oryzae (a. oryzae xlnr gene) resulted in elevated xylanolytic and cellulolytic activities in the culture supernatant, in which nearly 40 secreted proteins were detected by two-dimensional electrophoresis. dna microarray analysis to identify the transcriptional targets of aoxlnr led to the identification of 75 genes that showed more than fivefold i ... | 2009 | 19777228 |
| aspergillus oryzae atfa controls conidial germination and stress tolerance. | we compared atfa and atfb, the genes encoding the respective atf/creb-type transcription factors in aspergillus oryzae. the germination ratio of deltaatfa conidia was low without any stress, unlike that of deltaatfb conidia. the deltaatfa conidia were more sensitive to oxidative stress than the deltaatfb conidia, which are also sensitive to oxidative stress. we compared the gene expressions of these strains by using a dna microarray, genechip. almost all the genes regulated by atfb were also reg ... | 2009 | 19770065 |
| characterization and kinetic analysis of a thermostable gh3 beta-glucosidase from penicillium brasilianum. | a gh3 beta-glucosidase (bgl) from penicillium brasilianum was purified to homogeneity after cultivation on a cellulose and xylan rich medium. the bgl was identified in a genomic library, and it was successfully expressed in aspergillus oryzae. the bgl had excellent stability at elevated temperatures with no loss in activity after 24 h of incubation at 60 degrees c at ph 4-6, and the bgl was shown to have significantly higher stability at these conditions in comparison to novozym 188 and to other ... | 2010 | 19756584 |
| production and characterization of a milk-clotting enzyme from aspergillus oryzae mtcc 5341. | microbial milk-clotting enzymes are valued as calf rennet substitutes in the cheese industry. aspergillus oryzae mtcc 5341 was identified to produce the highest milk-clotting activity during screening of 16 fungal strains. solid state fermentation using wheat bran along with 4% defatted soy flour and 2% skim milk powder as substrate was optimal for growth of a. oryzae and production of the enzyme. nearly 40,000 u/g bran of milk-clotting activity was present at the end of 120 h. the enzyme could ... | 2010 | 19727708 |
| endocytic recycling at the tip region in the filamentous fungus aspergillus oryzae. | recent live cell imaging analyzing the components required for endocytosis has elucidated that endocytosis actively occurs at the hyphal tip region in filamentous fungi. to examine further the physiological roles of endocytosis we investigated a conditional mutant of endocytosis in aspergillus oryzae. endocytosis-deficient hyphae displayed retarded apical growth, abnormal hyphal morphology, mislocalization of a vesicle- snare, which is thought to undergo endocytic recycling to the tip region, an ... | 2009 | 19721880 |
| genome-wide analysis of maltose utilization and regulation in aspergilli. | maltose utilization and regulation in aspergilli is of great importance for cellular physiology and industrial fermentation processes. in aspergillus oryzae, maltose utilization requires a functional mal locus, composed of three genes: malr encoding a regulatory protein, malt encoding maltose permease and mals encoding maltase. through a comparative genome and transcriptome analysis we show that the mal regulon system is active in a. oryzae while it is not present in aspergillus niger. in order ... | 2009 | 19696104 |
| isolation of a new metabolite from biotransformation of daidzein by aspergillus oryzae. | two compounds were isolated from biotransformation of daidzein, a soybean isoflavone, by aspergillus oryzae. one was 6,7,8,4'-tetrahydroxyisoflavone, an a-ring dihydroxylated daidzein, and the other was a novel compound which had the cleaved b-ring of daidzein. the former was perhaps derived from 8-hydroxydaidzein and/or 6-hydroxydaidzein, and had high dpph radical-scavenging activity. | 2009 | 19661709 |
| nonhomologous end-joining deficiency allows large chromosomal deletions to be produced by replacement-type recombination in aspergillus oryzae. | gene targeting is a technique of introducing a genetic trait at a predetermined site within a genome; it is also used to eliminate undesirable chromosomal regions from the relevant genome. thus far, replacement-type recombination between two homologous regions separated by a large nonhomologous sequence has been hardly achieved probably due to the low frequency of homologous recombination in filamentous fungi. in this study, we report the successful and highly efficient deletion by replacement-t ... | 2009 | 19654050 |
| characterization of a 52 kda exoantigen of penicillium chrysogenum and monoclonal antibodies suitable for its detection. | the indoor clade of penicillium chrysogenum, the so-called fleming clade, is the most common species of penicillium on moldy building materials. in a previous study, we identified a 52 kda human antigen characteristic of the indoor clade of p. chrysogenum not present in a taxonomically diverse selection of fungi. further investigations revealed that it is a modestly glycosylated mature protein with a pi 5.3. the protein is apparently identical to a glucoamylase previously reported from an alumin ... | 2010 | 19653120 |
| characterization of a neutral ceramidase orthologue from aspergillus oryzae. | ceramide is an important molecule not only structurally but also regulationally as a modulator of various cellular events. ceramidase (cdase) are classified into three different types (acid, alkaline, and neutral cdases). neutral cdase could play an important role in the regulation of ceramide levels in the extracellular space. in this study, we describe the characterization of a neutral cdase orthologue from the filamentous fungus aspergillus oryzae. the gene encoding the neutral cdase ortholog ... | 2009 | 19650849 |
| identification of a basic helix-loop-helix-type transcription regulator gene in aspergillus oryzae by systematically deleting large chromosomal segments. | we previously developed two methods (loop-out and replacement-type recombination) for generating large-scale chromosomal deletions that can be applied to more effective chromosomal engineering in aspergillus oryzae. in this study, the replacement-type method is used to systematically delete large chromosomal dna segments to identify essential and nonessential regions in chromosome 7 (2.93 mb), which is the smallest a. oryzae chromosome and contains a large number of nonsyntenic blocks. we constr ... | 2009 | 19633118 |
| a food-grade enzyme preparation with modest gluten detoxification properties. | celiac sprue is a life-long disease characterized by an intestinal inflammatory response to dietary gluten. a gluten-free diet is an effective treatment for most patients, but accidental ingestion of gluten is common, leading to incomplete recovery or relapse. food-grade proteases capable of detoxifying moderate quantities of dietary gluten could mitigate this problem. | 2009 | 19621078 |
| cloning of aspergillus oryzae aovps5 gene, homologous to vacuolar protein sorting associated gene vps5 and construction of the disruptant. | aovps5 gene was isolated from aspergillus oryzae as a homologue to s. cerevisiae vps5 gene which encodes a polypeptide consisting of 451 amino acids that is nearly 32% homologous to vps5p. three aovps5 gene disruptants were generated and they showed higher activity of tripeptidyl peptidase, which is mainly detected in vacuoles, in their culture medium. higher amount of nitrogenous constituent was found in the filtrate of wheat gluten degraded by addition of culture medium of these disruptants th ... | 2009 | 19619858 |
| release of antihypertensive peptides in miso paste during its fermentation, by the addition of casein. | casein hydrolysate by aspergillus oryzae protease has been developed to obtain two kinds of antihypertensive peptides, val-pro-pro and ile-pro-pro. in this study, casein miso paste was prepared by adding casein at various concentrations during miso paste fermentation in order to release angiotensin-i converting enzyme (ace) inhibitory peptides. increase of peptide concentration and ace inhibitory activity in casein miso paste was observed and corresponded to the degradation of casein and soy bea ... | 2009 | 19619856 |
| post-genomic insights into the plant polysaccharide degradation potential of aspergillus nidulans and comparison to aspergillus niger and aspergillus oryzae. | the plant polysaccharide degradative potential of aspergillus nidulans was analysed in detail and compared to that of aspergillus niger and aspergillus oryzae using a combination of bioinformatics, physiology and transcriptomics. manual verification indicated that 28.4% of the a. nidulans orfs analysed in this study do not contain a secretion signal, of which 40% may be secreted through a non-classical method.while significant differences were found between the species in the numbers of orfs ass ... | 2009 | 19618505 |
| d-lactic acid production from cellooligosaccharides and beta-glucan using l-ldh gene-deficient and endoglucanase-secreting lactobacillus plantarum. | in order to achieve direct fermentation of an optically pure d: -lactic acid from cellulosic materials, an endoglucanase from a clostridium thermocellum (cela)-secreting plasmid was introduced into an l: -lactate dehydrogenase gene (ldhl1)-deficient lactobacillus plantarum (ldhl1) bacterial strain. cela expression and its degradation of beta-glucan was confirmed by western blot analysis and enzyme assay, respectively. although the cela-secreting ldhl1 assimilated cellooligosaccharides up to cell ... | 2010 | 19597813 |
| [promotive excretion of causative agents of yusho by intake of fermented brown rice with aspergillus oryze in patients with yusho --with regard to pcdfs and pcdds]. | forty years have passed since the outbreak of kanemi rice oil poisoning, namely, yusho in the western japan. however, even now the patients with yusho have been still suffering from several objective and subjective symptoms. in order to improve or, if possible, to cure such symptoms, the most important therapeutic treatment is considered to actively excrete the causative agents, that is, polychlorinated dibenzofurans (pcdfs) and polychlorinated dibenzo-p-dioxins (pcdds) from the bodies of the pa ... | 2009 | 19588849 |
| responses of aspergillus oryzae to stimuli from near-uv light irradiation in the presence or absence of titanium dioxide. | the responses of aspergillus oryzae to the stimuli from near-uv irradiation were investigated by using a black light fluorescent lamp in the presence or absence of tio2 particles. light irradiation at an intensity of 6 w/m2 strongly inhibited the growth of germinated pellets of a. oryzae. this growth inhibition was weakened by tio2 particles (0.05 g/l), especially in the initial growth phase, in which the expression level of catalase gene (catb) was approximately three times higher than that in ... | 2009 | 19579656 |
| heterologous expression and characterization of cpi, ocpa, and novel serine-type carboxypeptidase ocpb from aspergillus oryzae. | in the genome of aspergillus oryzae, 12 genes have been predicted to encode serine-type carboxypeptidases. however, the carboxypeptidase activities of the proteins encoded by these genes have not yet been confirmed experimentally. in this study, we have constructed three of these 12 genes overexpressing strains using aspergillus nidulans and characterized their overproduced recombinant proteins. of these three genes, one was previously named cpi; the other two have not been reported yet, and hen ... | 2009 | 19557408 |
| modified electrophoretic and digestion conditions allow a simplified mass spectrometric evaluation of disulfide bonds. | proper formation of disulfide bonds in proteins is a prerequisite to their stability and function. information on disulfide pattern may therefore serve as an indication of the proper folding of recombinant proteins, and can also be used in protein homology modeling for the purpose of structure refinement. protein handling and digestion at basic ph leads to disulfide bond scrambling. that is why the samples are usually treated and digested at low ph where no scrambling occurs. unfortunately, the ... | 2009 | 19551705 |
| cereal-based biorefinery development: utilisation of wheat milling by-products for the production of succinic acid. | a novel wheat-based bioprocess for the production of a nutrient-complete feedstock for the fermentative succinic acid production by actinobacillus succinogenes has been developed. wheat was fractionated into bran, middlings and flour. the bran fraction, which would normally be a waste product of the wheat milling industry, was used as the sole medium in two solid-state fermentations (ssf) of aspergillus awamori and aspergillus oryzae that produce enzyme complexes rich in amylolytic and proteolyt ... | 2009 | 19539669 |
| functional analysis and subcellular localization of two geranylgeranyl diphosphate synthases from penicillium paxilli. | the filamentous fungus penicillium paxilli contains two distinct geranylgeranyl diphosphate (ggpp) synthases, ggsa and ggsb (paxg). paxg and its homologues in neotyphodium lolii and fusarium fujikuroi are associated with diterpene secondary metabolite gene clusters. the genomes of other filamentous fungi including aspergillus fumigatus, aspergillus nidulans, aspergillus niger, aspergillus oryzae and fusarium graminearum also contain two or more copies of ggpp synthase genes, although the diterpe ... | 2009 | 19529962 |
| is electron equivalence between substrate and product preferable to c-mol equivalence in representations of microbial anabolism applicable to "origin of life" environmental conditions. | this research compares two techniques for constructing sets of equations representing the growth of a microorganism on a given substrate. each anabolic equation represents a defined initial and a final state. the initial state comprises all necessary mineral nutrients and one organic source of carbon and energy. the final state comprises the microbial cells that have grown, and any additional organic or inorganic products of the growth process. the cells are represented by a unit-carbon formula ... | 2009 | 19501106 |
| a unique post-translational processing of an exo-beta-1,3-glucanase of penicillium sp. kh10 expressed in aspergillus oryzae. | to characterize an exo-beta-1,3-glucanase (exgp) of an isolated fungal strain with high laminarin degradation activity, identified as penicillium sp. kh10, heterologous secretory expression of the exgp was performed in aspergillus oryzae. deduced amino acid sequence of the exgp gene possibly consisted of 989 amino acids which showed high sequence similarity to those of fungal exo-beta-1,3-glucanases belonging to the glycoside hydrolase (gh) family 55. notably, the purified recombinant exgp showe ... | 2009 | 19500674 |
| alpha-lactalbumin hydrolysate stimulates glucagon-like peptide-2 secretion and small intestinal growth in suckling rats. | we investigated whether bovine milk constituents influenced glucagon-like peptide (glp)-2 secretion and intestinal growth in suckling rats. male sprague-dawley rats (14 d old) received i.g. infusions of a milk protein fraction, a lactose solution, or the cream fraction of milk. the serum concentration of glp-2, but not glp-1, markedly increased in rats administered milk protein compared with those given the lactose solution or the cream fraction from 60 to 120 min after administration. in anothe ... | 2009 | 19494023 |
| crystallization of carbohydrate oxidase from microdochium nivale. | microdochium nivale carbohydrate oxidase was produced by heterologous recombinant expression in aspergillus oryzae, purified and crystallized. the enzyme crystallizes with varying crystal morphologies depending on the crystallization conditions. several different crystal forms were obtained using the hanging-drop vapour-diffusion method, two of which were used for diffraction measurements. hexagon-shaped crystals (form i) diffracted to 2.66 a resolution, with unit-cell parameters a = b = 55.7, c ... | 2009 | 19478452 |
| comparison of stirred tank and airlift bioreactors in the production of polygalacturonases by aspergillus oryzae. | the production of endo and exo-polygalacturonase (pg) by aspergillus oryzae ipt 301 was studied in a stirred tank bioreactor (str) and an internal circulation airlift bioreactor. using a factorial experimental design, a soluble culture medium was defined which allowed the production of exo- and endo-pg comparable to that obtained in a medium containing suspended wheat bran. the soluble medium was used in tests to compare the production of these enzymes in the str and airlift bioreactor. in these ... | 2009 | 19467860 |
| an acidophilic beta-galactosidase from bispora sp. mey-1 with high lactose hydrolytic activity under simulated gastric conditions. | bgala, a full-length gene (3,009 bp) that encodes a beta-galactosidase, was cloned from the meso-acidophilic fungus bispora sp. mey-1 and expressed in pichia pastoris. the deduced amino acid sequence of bgala shares highest identity (55.5%) with the beta-galactosidase from aspergillus phoenicis, which belongs to the glycoside hydrolyase family 35. purified recombinant bgala is acidophilic, exhibiting maximum activity at ph 1.5, which is lower than that reported for other beta-galactosidases. the ... | 2009 | 19453169 |
| purification and identification of proteolytic enzymes from aspergillus oryzae capable of producing the antihypertensive peptide ile-pro-pro. | two proteolytic enzymes capable of releasing the angiotensin i-converting enzyme (ace) inhibitor ile-pro-pro from casein were identified by purification of an aspergillus oryzae extract by three-step column chromatography. first, proteins capable of producing ile-pro-pro from beta-casein were eluted using a deae-sepharose ff column with a linear sodium chloride gradient. an endopeptidase capable of releasing pro-ile-pro-gln-ser-leu-pro-gln-asn-ile-pro-pro from pro-ile-pro-gln-ser-leu-pro-gln-asn ... | 2009 | 19447337 |
| pepj is a new extracellular proteinase of aspergillus nidulans. | under carbon starvation, aspergillus nidulans released a metallo-proteinase with activities comparable to those of prta, the major extracellular serine proteinase of the fungus. the relative molar mass of the enzyme was 19 kda as determined with both denaturing and renaturing sds page, while its isoelectric point and ph and temperature optima were 8.6, 5.5 and 65 degrees c, respectively. the enzyme was stable at ph 3.5-10.5 and was still active at 95 degrees c in the presence of azocasein substr ... | 2009 | 19418246 |
| functional expression of the aspergillus flavus pks-nrps hybrid cpaa involved in the biosynthesis of cyclopiazonic acid. | alpha-cyclopiazonic acid (cpa) is an indole tetramic acid mycotoxin. based on our identification of the polyketide synthase-nonribosomal peptide synthase (pks-nrps) hybrid gene cpaa involved in cyclopiazonic acid biosynthesis in aspergillus fungi, we carried out heterologous expression of aspergillus flavuscpaa under alpha-amylase promoter in aspergillus oryzae and identified its sole product to be the cpa biosynthetic intermediate cyclo-acetoacetyl-l-tryptophan (caatrp). this result rationalize ... | 2009 | 19410456 |
| heterologous expression and characterization of an endoglucanase from a symbiotic protist of the lower termite, reticulitermes speratus. | rssymeg, an endoglucanase of glycosyl hydrolase family (ghf) 7 encoded by a transcript isolated from the symbiotic protist of the termite reticulitermes speratus, is expressed in aspergillus oryzae. interestingly, purified rssymeg1 has a relatively higher specific activity (603 micromol min(-1) mg(-1) protein) and v(max) value (769.6 unit/mg protein) than previously reported data for ghf7 endoglucanase of trichoderma ressei. it also has the same k(m) value (1.97 mg/ml) with clostridium celluloly ... | 2010 | 19404781 |
| genetic analysis of glucosidase ii beta-subunit in trimming of high-mannose-type glycans. | glucosidase ii (g-ii) is a glycoprotein-processing enzyme that successively cleaves two alpha1,3-linked glucose residues from n-linked oligosaccharides in the endoplasmic reticulum. g-ii is a heterodimer whose alpha-subunit contains a glycosidase active site, but the function(s) of the beta-subunit remain poorly defined. we report here an in vivo enzymatic analysis using gene disruptants lacking either the g-ii alpha- or beta-subunit in the filamentous fungus aspergillus oryzae. using synthetic ... | 2009 | 19395677 |
| a biotechnological product and its potential as a new immunomodulator for treatment of animal phlebovirus infection: punta toro virus. | intracellular pathogens with widespread drug-resistance contribute substantially to the increasing rates in morbidity and mortality due to emerging and reemerging diseases. thus, the development of new drugs, including those that can enhance the immune response, is urgently needed. the immunomodulator, p-mapa, a proteinaceous aggregate of ammonium and magnesium phospholinoleate-palmitoleate anhydride derived from aspergillus oryzae, have been shown to induce antitumor activities. the ability of ... | 2009 | 19393266 |
| evaluating the potential for enzymatic acrylamide mitigation in a range of food products using an asparaginase from aspergillus oryzae. | asparaginase, an enzyme that hydrolyzes asparagine to aspartic acid, presents a potentially very effective means for reducing acrylamide formation in foods via removal of the precursor, asparagine, from the primary ingredients. an extracellular asparaginase amenable to industrial production was cloned and expressed in aspergillus oryzae . this asparaginase was tested in a range of food products, including semisweet biscuits, ginger biscuits, crisp bread, french fries, and sliced potato chips. in ... | 2009 | 19388639 |
| atan1p-an extracellular tannase from the dimorphic yeast arxula adeninivorans: molecular cloning of the atan1 gene and characterization of the recombinant enzyme. | the tannase-encoding arxula adeninivorans gene atan1 was isolated from genomic dna by pcr, using as primers oligonucleotide sequences derived from peptides obtained after tryptic digestion of the purified tannase protein. the gene harbours an orf of 1764 bp, encoding a 587-amino acid protein, preceded by an n-terminal secretion sequence comprising 28 residues. the deduced amino acid sequence was similar to those of tannases from aspergillus oryzae (50% identity), a. niger (48%) and putative tann ... | 2009 | 19387973 |