| restriction fragment length polymorphisms in isolates of aspergillus fumigatus probed with part of the intergenic spacer region from the ribosomal rna gene complex of aspergillus nidulans. | differences in restriction fragment length polymorphisms (rflps) have been detected in isolates of aspergillus fumigatus. genomic dna from 11 isolates was digested with ecori, separated by electrophoresis, southern blotted and probed with dna from the intergenic spacer or non-transcribed spacer region of the rrna gene complex of aspergillus nidulans. three distinct rflp patterns were detected which differed from the control patterns observed with a. nidulans, aspergillus flavus and aspergillus n ... | 1990 | 1980128 |
| moist heat intraluminal disinfection of capd connectors. | a moist heat technique for disinfecting the inner lumen of commercially available connectology used in the exchange process for continuous ambulatory peritoneal dialysis (capd) was evaluated. moist heat was generated by a device (pdm-1) that directed microwave energy to heat a sample solution containing a concentration of 10(6) microorganisms inside a pair of mated plastic capd connectors. microorganisms tested included those most prevalent and most problematic in causing peritonitis. testing, p ... | 1990 | 1982800 |
| purification and characterization of two endopolygalacturonases from sclerotinia sclerotiorum. | the endopolygalacturonase (ec 3.2.1.15) enzyme produced in vitro by sclerotinia sclerotiorum were found to consist of numerous isoforms covering a broad pi range. two of the isoforms, labelled pg2 and pg3, were purified using gel-filtration chromatography, isoelectric focusing and anion-exchange chromatography. the pis of pg2 and pg3 were, respectively, 4.8 and 4.9. their molecular weights were similar. both enzymes hydrolysed 0.9% of the bonds in reaching a 50% reduction in viscosity. however, ... | 1991 | 1991145 |
| transformation of the filamentous fungus gibberella fujikuroi using the aspergillus niger niad gene encoding nitrate reductase. | a transformation system for gibberella fujikuroi based on the aspergillus niger nitrate reductase gene (niad) was developed. a strain (designated sg140) carrying a non-reverting niad mutation (niad11) was generated by screening mutagenised cells for non-growth on nitrate as sole nitrogen source. transformation frequencies of 1-2 transformants per microgram dna were observed when strain sg140 was transformed to nitrate utilisation. southern blot analyses of niad+ transformants showed that the vec ... | 1991 | 2005864 |
| 15n- and 13c-nmr investigations of glucose oxidase from aspergillus niger. | the apoprotein of glucose oxidase from aspergillus niger was reconstituted with specifically 15n- and 13c-enriched fad derivatives and investigated by 15n- and 13c-nmr spectroscopy. on the basis of the 15n-nmr results it is suggested that, in the oxidized state of glucose oxidase, hydrogen bonds are formed to the n(3) and n(5) positions of the isoalloxazine system. the hydrogen bond to n(3) is more pronounced than that to n(5) as compared with the respective hydrogen bonds formed between fmn and ... | 1991 | 2013289 |
| an autonomously replicating plasmid transforms aspergillus nidulans at high frequency. | from an unstable aspergillus nidulans colony, resulting from transformation with an a. nidulans gene bank, a plasmid was reisolated which transformed a. nidulans at a frequency of 20,000 transformants per 10(6) protoplasts at near saturation levels of transforming dna. this represents a 250-fold enhancement of transformation efficiency over that found for typical integrative vectors such as pilj16, the plasmid used in gene bank constructions. the plasmid, designated arp1, is 11.5 kb in size, and ... | 1991 | 2013411 |
| ecotoxicological aspects of aspergilli present in the phylloplane of stored leaves of chewing tobacco (nicotiana tobaccum). | nine different species of aspergillus were isolated from the phylloplane of stored chewing tobacco (nicotiana tobaccum) of different ages. the maximum number of species were isolated from 12 and 18 month old leaves. a. ruber, a. ochraceus, a. flavus and a. nidulans were usually associated with older leaves while a. niger, a. fumigatus and a. flavus were isolated from 6 month old leaves. approximately 18% of aspergilli were found to be mycotoxigenic. sterigmatocystin was produced by three differe ... | 1991 | 2014047 |
| nucleotide sequence of pnl gene from erwinia carotovora er. | the nucleotide sequence of pnl gene encoding pectin lyase (pnl; ec4.2.2.10)from erwinia carotovora er was determined. the structural gene of pnl consisted of 942 base pairs. an open reading frame that could encode a 33,700 dalton polypeptide consisting 314 amino acids was assigned. the molecular size of the polypeptide predicted from the amino acid composition was close to the value of pnl determined in e.carotovora er. the nucleotide sequence of the 5'-flanking region showed the presence of the ... | 1991 | 2018526 |
| [antimicrobial activity of various algae of the panamanian atlantic coast]. | the methanolic extracts in 5 of 7 alagae from the atlantic coast of panama: caulerpa racemosa, halimeda opuntia, gelidiela acerosa, laurencia papillosa y acanthophora spicifera, showed antimicrobial activity against staphylococcus aureus and bacillus subtilis in a concentration of 50 mg/ml by the cylinder plate method. none of the algae studies showed activity against escherichia coli, pseudomonas aeruginosa, aspergillus niger and candida albicans. the content of agar in acanthophora spicifera w ... | 1991 | 2024058 |
| interaction of beta-cyclodextrin with the granular starch binding domain of glucoamylase. | the granular starch binding domain of glucoamylase 1 (ec 3.2.1.3 1,4-alpha-d-glucan glucohydrolase) binds two molecules of beta-cyclodextrin, with a dissociation constant (kd) for the second ligand of 1.68 microm. the catalytic domain showed no interaction with beta-cyclodextrin. beta-cyclodextrin competitively inhibited the adsorption of the binding domain onto granular starch with an inhibition constant (ki) of 11.0 +/- 1.9 microm. the results show that beta-cyclodextrin binds to the binding d ... | 1991 | 2049377 |
| antimicrobial and cytotoxic activity of transition metal chelates with some heterocyclic imines. | cu(ii), ni(ii), co(ii), and zn(ii) chelates with two heterocyclic imines derived from 2-furylglyoxal-2(1)-aminothiophenol (fgatp) and 2-thiophenylglyoxal-anthranilicacid (tgaa) were synthesized. elemental analysis, molar conductance, magnetic measurements and ir and electronic spectral data were explored to elucidate their probable structures. different crystal field parameters were also calculated to ascertain the geometry of the resulting chelates. all the ligands and their metal chelates were ... | 1991 | 2056311 |
| bioenergetic consequences of glucoamylase production in carbon-limited chemostat cultures of aspergillus niger. | aspergillus niger has been grown in glucose- and maltose-limited continuous cultures to determine the bioenergetic consequences of the production of the extracellular enzyme glucoamylase. growth yields (g biomass per mol substrate) were high, indicating that growth was very efficient and protein production for biomass was not exceedingly energy consuming. it has been found that the energy costs for the production of this extracellular enzyme is very high. depending on the efficiency of energy co ... | 1991 | 2059009 |
| synthetic and pharmacological studies on some transition metal chelates involving n-pyrimidino benzamide-2-carboxylic acid as ligand. | n-pyrimidino benzamide-2-carboxylic acid (npbca) and its cu(ii), ni(ii), co(ii), zn(ii), and mn(ii) chelates have been synthesized and characterized by using elemental analyses, molar conductance, molecular weight determination, magnetic moment, infrared, and electronic spectra. antifungal activity of the synthesized compounds has been screened on common fungi, viz., aspergillus niger, aspergillus nidulense, and candida albicans at 28 degrees c and antibacterial activity has been observed on gra ... | 1991 | 2066740 |
| single cell protein production by aspergillus niger and its evaluation. | an efficient cellulolytic mold, aspergillus niger as-101 was used to produce single cell protein from alkalitreated corn cobs. microbial biomass obtained after 6 days of fermentation was evaluated for its various quality components. single cell protein product contained 30.4% crude protein and had an essential amino acid profile featuring a high lysine content and appreciable amounts of methionine and tryptophane. product contained 12.9% fat which was comprised of all essential fatty acids. prod ... | 1991 | 2068872 |
| immobilization of glucose oxidase by electropolymerization of monomers. influence of polymerization conditions. | | 1990 | 2075994 |
| expression of the aspergillus niger glucose oxidase gene in a. niger, a. nidulans and saccharomyces cerevisiae. | we report the cloning of the aspergillus niger glucose oxidase gene and its use to elevate glucose oxidase productivity in a. niger by increasing the gene dosage. in addition, the gene has been introduced into a. nidulans where it provides the novel capacity to produce glucose oxidase. a plasmid, in which dna encoding the mature form of glucose oxidase was preceded by a saccharomyces cerevisiae secretion signal, effected high-level production of extracellular glucose oxidase in this yeast. | 1990 | 2076553 |
| regulation of the glaa gene of aspergillus niger. | the glucoamylase gene of aspergillus niger, glaa, is expressed at high levels in the presence of starch. we have determined the nucleotide sequence of 1966 bp of the 5' flanking region of the glaa gene and have begun to identify sequences important for the control of glaa expression by deletion analysis. constructs containing deletions extending into the glaa gene promoter were introduced into an a. niger host whose own glaa gene had been disrupted by a gene replacement event. secreted levels of ... | 1990 | 2076554 |
| fungi of virgin and cultivated soil of salhiah desert, egypt. | 27 species and 13 genera of fungi were identified from virgin and cultivated soil of salhiah. the most abundant species of phosphate solubilizing fungi were aspergillus nidulans, a. niger, a flavus, penicillium lilacinum, p. frequentans and fusarium moniliforme. on cellulose agar the most prevalent species were chaetomium bostrychodes, c. olivaceum, humicola fuscoatra, aspergillus flavus, a. nidulans, a. niger, a. ochraceus, fusarium solani and f. oxysporum. on xylan agar aspergillus fumigatus, ... | 1990 | 2077791 |
| genetic localization of a series of genes affecting glucose oxidase levels in aspergillus niger. | a number of mutants of aspergillus niger, affected in glucose oxidase (gox) expression, are described. the overproducing mutants could be classified into seven complementation groups whereas two glucose oxidase-negative complementation groups were recognized. these nine gox loci were assigned to linkage groups using master strains with marked chromosomes. three gox loci are in linkage group ii, one is in iii, two are in v and two are in linkage group vii. one weak glucose oxidase-overproducing m ... | 1990 | 2078868 |
| evaluation of antifungal volatile compounds on the basis of the elongation rate of a single hypha. | a novel method is proposed for the evaluation of the activity of an antifungal agent administered as a gas. this system is composed of a batch-flow type reaction vessel, a gas flow system, and a microscopic observation system. the agar plate was prepared on the ceiling of the reaction vessel, and the mycelium of a fungus (aspergillus niger or rhizoctonia solani) was inoculated onto it. after preincubation at 25 degrees c for 24 h, the reaction vessel was connected to the gas flow system. an appr ... | 1990 | 2082824 |
| peritonitis due to aspergillus niger: diagnostic importance of peritoneal eosinophilia. | | 1990 | 2085565 |
| l-ascorbic acid alpha-glucoside formed by regioselective transglucosylation with rat intestinal and rice seed alpha-glucosidases: its improved stability and structure determination. | the definite structure and chemical stability of a new glucoside of l-ascorbic acid (aa) which was enzymatically glucosylated with rat intestinal and rice seed alpha-glucosidases were reported. the stability of this aa derivative in water under aerobic conditions was proved by its remarkable resistance against enhanced oxidative degradation by heat, cu2+ ion or ascorbate oxidase, and it was found to have no reducing activity toward radicals. these properties were obviously distinguishable from t ... | 1990 | 2085881 |
| fungal flora in congenital dacryocystitis. | in this study, 86 eyes in 66 cases (20 bilateral) of congenital dacryocystitis were analysed for fungal growth. fungi alone were isolated in 12 eyes (13.95%) and in 14 eyes (16.28%) together with bacteria--a total of 26 positive for fungus in 86 eyes (30.23%). these 26 eyes yielded on fungal culture a total of 28 isolates (in 2 eyes, another fungus was isolated on repeat culture). 11 types of fungi were cultured--c. albicans and a. niger accounted for 5 each out of 28. to the best of our knowled ... | 1990 | 2086475 |
| syntheses, characterization, and microbial activity of some transition metal complexes involving potentially active o and n donor heterocyclic ligands. | the formation of binary as well as ternary metal complexes of type mll' (where m(ii) = cu(ii), ni(ii), co(ii), and zn(ii); l = 8-hydroxyquinoline, and l' = 2-furoic acid) has been studied. the complexes were synthesized and characterized by elemental analyses, molecular weight determination, the ir and electronic spectra, conductivity, and magnetic measurements. the presence of coordinated water molecules was demonstrated by thermogravimetric analysis. the microbial activity of these ligands and ... | 1990 | 2086721 |
| microbiological transformation of chromone, chromanone, and ring a hydroxyflavones. | microbial transformation of chromone, chromanone, and 3 ring a hydroxyflavones (5-hydroxy-, 6-hydroxy-, and 7-hydroxyflavones) was attempted using thirty-two microorganisms. while chromone was not biotransformed, chromanone was transformed to chromone and chromanol by aspergillus niger in 2% yield. ring a hydroxylated flavones were transformed to the corresponding c-4' hydroxylated metabolites, and the yields and rate of product formation were found to correlate with the distance between the c-4 ... | 1990 | 2089118 |
| tomato and aspergillus niger pectinesterases. correlation of differences in existing reports: large species variations. | existing reports on the enzyme tomato pectinesterase differ in alignments of two internal segments and in many single-residue replacements. the alignment from cdna data, with a 317-residue mature protein, is correct, but protein analyses also show at least 18 single-residue replacements, suggesting the presence of many native or (less likely) cloning-derived microheterogeneities. purification of aspergillus niger pectinesterase and n-terminal sequence analysis of this enzyme reveal a different s ... | 1990 | 2089377 |
| investigations of the activity by contact of miconazole sulfosalicylate. | the in-vitro antimicrobial activity of miconazole sulfosalicylate (m.ssa) has been investigated on mycetes (candida albicans, cryptococcus neoformans, aspergillus niger, trichophyton mentagrophytes), gram-positive bacteria (staphylococcus aureus, streptococcus faecalis) and gram-negative bacteria (escherichia coli, pseudomonas aeruginosa, serratia marcescens and proteus vulgaris) in comparison with miconazole nitrate (m.nit). the results showed m.ssa has a greater activity than m.nit, particular ... | 1990 | 2093108 |
| evidence that the m antigen of histoplasma capsulatum var. capsulatum is a catalase which exhibits cross-reactivity with other dimorphic fungi. | a panel of monoclonal antibodies (mabs) was raised against histoplasmin, the antigen derived from the mycelial phase of histoplasma capsulatum var. capsulatum which contains the diagnostically useful h and m antigens. a number of mabs were obtained which recognized a 70-75 kd component of an antigenic preparation of h. capsulatum var. capsulatum by western blotting. when reacted with histoplasmin by western blotting the mabs recognized a similar 70-75 kd band, together with a series of higher mo ... | 1990 | 2093121 |
| studies on heterocyclic compounds: spiro [indole-3,2'-thiazolidine] derivatives. antimicrobial activity of monohalogenated 3'-phenylspiro 3h-indole-3,2'-thiazolidine-2,4' (1h)-diones. | the following halogenated 3'-phenyl [3h-indole-3,2'-thiazolidine]-2,4'(1h)-dione of general formula (a) were synthesized and screened for antimicrobial activity. (formula: see text) where: x = h (i, iii, v, vii, ix, xi, xiii, xv), ch3 (ii, iv, vi, viii, x, xii, xiv, xvi); y = h (i, ii), 3-f (iii, iv), 2-cl (v, vi), 3-cl (vii, viii), 4-cl (ix, x), 2-br (xi, xii), 3-br (xiii, xiv), 4-br (xv, xvi). the synthetic approach involves the preparation of variously substituted schiff-bases of indol-2,3-di ... | 1990 | 2100530 |
| studies on heterocyclic compounds: spiro [indole-3,2'-thiazolidine] derivatives. ii. antimicrobial activity of halogenated 3'-phenylspiro 3h-indole-3,2'-thiazolidine -2,4 (1h)-diones. | the following polyhalogenated 3'-phenyl 3h-indole-3,2'-thiazolidine -2,4' (1h)-dione of general formula (a) were synthesized and screened for antimicrobial activity. (formula: see text) where: x = h (i, iii, v, vii, ix, xi), ch3 (ii, iv, vi, viii, x, xii); y = h (i, ii), 2,4-f2 (iii, iv), 2,4-cl2 (v, vi), 3,4-cl2 (vii, viii), 2,6-cl2 (ix, x), 2,4,6-cl3 (xi, xii). the general synthetic route involves the preparation of variously substituted isatin-3-imines, which are subjected to cyclocondensatio ... | 1990 | 2100531 |
| houses and allergic respiratory syndromes. | a study was carried out in 49 flats in bucharest inhabited by 265 persons. a quantitative analysis of fungi spores was performed as well as allergologic tests with penicillium and aspergillus fungi (a13), aspergillus niger (m2), alternaria tenuis (m1), cladosporium herbarum (m3), dermatophagoides pteronissinus (allergenic products supplied by the bencard firm). the infested flats presented a high percentage of aspergillus niger spores, particularly pathogenic. the main triggering factors for the ... | 1990 | 2100879 |
| comparison of the properties of glucoamylases from rhizopus niveus and aspergillus niger. | some properties of the glucoamylase from rhizopus niveus have been determined and compared with the comparable properties of the glucoamylase from aspergillus niger. the enzymes from these organisms possess the following common properties: quantitative conversion of starch to glucose, molecular weights in the range 95,500 to 97,500, and glycoprotein structures with many oligosaccharide side chains attached to the protein moieties of the enzymes. differences in the glucoamylases exist in electrop ... | 1990 | 2106901 |
| identification of carboxylic acid residues in glucoamylase g2 from aspergillus niger that participate in catalysis and substrate binding. | functionally important carboxyl groups in glucoamylase g2 from aspergillus niger were identified using a differential labelling approach which involved modification of the acarbose-inhibited enzyme with 1-ethyl-3-(4-azonia-4,4-dimethylpentyl)carbodiimide (eac) and inactivation by [3h]eac following removal of acarbose. subsequent sequence localization of the substituted acidic residues was facilitated by specific phenylthiohydantoins. the acid cluster asp176, glu179 and glu180 reacted exclusively ... | 1990 | 2108020 |
| interconversion of aflatoxin b1 and aflatoxicol by several fungi. | four fungal strains, namely, aspergillus niger, eurotium herbariorum, a rhizopus sp., and non-aflatoxin (af)-producing aspergillus flavus, which could convert af-b1 to aflatoxicol (afl), could also reconvert afl to af-b1. the interconversion of af-b1 to afl and of afl to af-b1 was ascertained to occur during proliferation of the fungi. these reactions were distinctly observed in cell-free systems obtained from disrupted mycelia of a. flavus and the rhizopus sp., but they were not observed in cul ... | 1990 | 2111122 |
| the promoter of the glucoamylase-encoding gene of aspergillus niger functions in ustilago maydis. | promoter sequences from the aspergillus niger glucoamylase-encoding gene (glaa) were linked to the bacterial hygromycin (hy) phosphotransferase-encoding gene (hph) and this chimeric marker was used to select hy-resistant (hyr) ustilago maydis transformants. this is an example of an ascomycete promoter functioning in a basidiomycete. hyr transformants varied with respect to copy number of integrated vector, mitotic stability, and tolerance to hy. only 216 bp of glaa promoter sequence is required ... | 1990 | 2112106 |
| upstream elements repress premature expression of an aspergillus developmental regulatory gene. | the aspergillus nidulans abaa gene regulates intermediate steps in asexual reproductive development and is itself developmentally regulated. an 822-base-pair dna fragment from the abaa 5'-flanking region is sufficient to drive developmentally appropriate expression of the escherichia coli lacz gene. deletion analysis showed that this fragment contains elements that repress transcription in vegetative cells and immature conidiophores and that activate transcription later during development. a 45- ... | 1990 | 2117702 |
| production and purification of a granular-starch-binding domain of glucoamylase 1 from aspergillus niger. | a domain of glucoamylase 1 from aspergillus niger which binds to granular starch was produced by proteolytic digestion and purified to apparent homogeneity by extraction with corn starch followed by anion-exchange chromatography and gel filtration. the peptide has a molecular weight of 25,100, contains approximately 38% carbohydrate (w/w) and corresponds to residues 471-616 at the c-terminus of glucoamylase 1. the peptide bound to granular corn starch maximally at 1.08 nmol/mg starch. it inhibit ... | 1990 | 2119316 |
| analysis of delta-l-alpha-aminoadipyl-l-cysteinyl-d-valine by ion chromatography and pulsed amperometric detection. | a novel high-performance liquid chromatography (hplc) method is presented for the detection and trace level determination of the tripeptide delta-l-alpha-aminoadipyl-l-cysteinyl-d-valine (acv). the tripeptide, an intermediate in penicillin production, is derived from fungal fermentation. the technique relies on ion-exchange separation of the tripeptide on an anion-exchange column followed by detection by reduction on a gold electrode using pulsed amperometry. the sensitivity of direct determinat ... | 1990 | 2120274 |
| influence of the addition of mineral salts on the inhibitory activity of strains of the arthrinium genus. | the influence which the addition of certain mineral salts has on the inhibiting activity of strains belonging to the arthrinium genus was studied. the salts employed were agno3, fecl2, mncl2 and cuso4, in concentrations of 10, 50, 100, 150 and 200 mg/l, in the presence of bacillus subtilis, enterobacter cloacae, staphylococcus aureus, escherichia coli, serratia marcescens, klebsiella pneumoniae, candida albicans and aspergillus niger. for b. subtilis and s. aureus greater inhibition was observed ... | 1990 | 2120549 |
| ultraviolet radiation for the sterilization of contact lenses. | two sources of ultraviolet (uv) radiation with peak wavelengths in the uv-c or uv-b ranges were compared for their ability to sterilize contact lenses infected with pseudomonas aeruginosa, streptococcus pneumoniae, acanthamoeba castellani, candida albicans, and aspergillus niger. also examined was the effect of prolonged uv light exposure on soft and rigid gas permeable (rgp) contact lenses. the uv-c lamp (253.7 nm, 250 mw/cm2 at 1 cm) was germicidal for all organisms within 20 minutes but cause ... | 1990 | 2123422 |
| behaviour of aspergillus flavus in presence of aspergillus niger during biosynthesis of aflatoxin b. | aspergillus niger or aspergillus tamarii when grown as mixed cultures with toxigenic a. flavus inhibits biosynthesis of aflatoxin by a. flavus, owing primarily to its ability to produce inhibitors of aflatoxin biosynthesis and to their ability to degrade aflatoxin. gluconic acid partly prevents aflatoxin production. the other factors such as changes in ph of the medium and the effect on the growth of a. flavus have no role in imparting capabilities to these cultures to inhibit aflatoxin producti ... | 1990 | 2124794 |
| [ear infections by aspergillus]. | report of 123 cases of otomycosis by aspergillus treated by the a. analyse of the several risks and causative factors, as well as the symptomatology and findings at examination. comments regarding the a. own protocol, pointing out the importance of daily dressings and early diagnosis. | 1990 | 2126916 |
| glycogen accumulation in aspergillus niger. | | 1990 | 2128057 |
| inactivity of terbinafine in a rat model of pulmonary aspergillosis. | in a model of bronchopulmonary aspergillosis terbinafine did not improve survival of experimental animals in doses up to 80 mg/kg/day despite adequate lung concentrations. pretreatment and aerosolization of the compound were also ineffective. terbinafine was markedly less active in vitro when serum was used instead of yeast-nitrogen-glucose-broth. it is concluded that a lack of bioavailability in the presence of serum may explain the lack of activity of terbinafine in experimental aspergillosis. | 1990 | 2128278 |
| [lung mycoses caused by opportunistic fungi]. | | 1990 | 2128860 |
| aspergillus species from hospital air and from patients. | during a seven month period, 367 molds were isolated from hospital air and 23 from patients. aspergillus niger accounted for 56% of air isolates, but for only 17% of patient isolates. aspergillus fumigatus was rarely found in air (0.3%), but was the dominant isolate among our patients (44%). we conclude, that different aspergillus species may have a varied aptitude to colonize immunocompromised patients. | 1990 | 2129435 |
| changes in the concentration of fructose 2,6-bisphosphate in aspergillus niger during stimulation of acidogenesis by elevated sucrose concentration. | the presence of fructose 2,6-bisphosphate (fru-2,6-p2) and phosphofructokinase 2 (pfk 2) were established in the citric-acid-producing filamentous fungus aspergillus niger. fru-2,6-p2 levels were around 3.0 (+/- 0.8) nmol per g dry weight during growth on sucrose, and half of this in mycelia grown on citrate as a carbon source. pfk 2 was detected with a specific activity of 150 mu/mg protein and a km for fructose 6-phosphate of 40 microm. induction of citric acid accumulation (acidogenesis) in a ... | 1990 | 2156568 |
| mycoflora and nutritional value of shelled melon seeds (citrulus vulgaris schrad.) in nigeria. | thirteen fungi were isolated from mouldy shelled melon seeds. the fungi more frequently isolated included species of mucor (11.25%), rhizopus (13.75%), aspergillus (36.25%), macrophoma (2.50%), penicillium (8.75%), alternaria (5.00%), fusarium (8.75%), botrytis (6.25%), torula (3.75%) and geotrichum (3.75%). healthy, shelled melon seeds inoculated with fusarium solani for 7 or 14 days caused increases in the free fatty acid (f.f.a.) content of the healthy seeds from an initial value of 1.06% to ... | 1990 | 2170965 |
| localized peripheral calcium oxalate crystal deposition caused by aspergillus niger infection. | a 70-year-old white man, who was malnourished and demented, presented with a painful black, gangrenous-appearing right foot with a fungating mass and required amputation. pathological sections of the tissue specimens showed numerous birefringent crystals, which were identified as calcium oxalate. branching septate hyphae and darkly pigmented fungal fruiting heads with double sterigmata characteristic of aspergillus niger were also identified in the tissue sections. no systemic or other metabolic ... | 1990 | 2185361 |
| hydrolysis of beta-d-glucopyranosyl fluoride to alpha-d-glucose catalyzed by aspergillus niger alpha-d-glucosidase. | aspergillus niger alpha-d-glucosidase, crystallized and free of detectable activity for beta-d-glucosides, catalyzes the slow hydrolysis of beta-d-glucopyranosyl fluoride to form alpha-d-glucose. maximal initial rates, v, for the hydrolysis of beta-d-glucosyl fluoride, p-nitrophenyl alpha-d-glucopyranoside, and alpha-d-glucopyranosyl fluoride are 0.27, 0.75, and 78.5 mumol.min-1.mg-1, respectively, with corresponding v/k constants of 0.0068, 1.44, and 41.3. independent lines of evidence make cle ... | 1990 | 2191775 |
| selection of a hyperproducing strain of aspergillus niger for biosynthesis of citric acid on unusual carbon substrates. | several tens of mutants were obtained by uv irradiation of a spore suspension of aspergillus niger. producers yielding large amounts of citric acid were selected on a modified czapek agar containing methyl red as ph indicator. after a submerged cultivation in flasks with baffles, a mutant characterized by yellow pigmentation on wort agar and by yields of citric acid up to 74.6% in the medium containing glucose was chosen from 130 isolates tested. | 1990 | 2199351 |
| formation of a stable l-ascorbic acid alpha-glucoside by mammalian alpha-glucosidase-catalyzed transglucosylation. | enzymatic transglucosylation from maltose to l-ascorbic acid (aa) with mammalian tissue homogenates was determined by a high-performance liquid chromatography method and compared with the reaction catalyzed by alpha-glucosidase from aspergillus niger. the homogenates of small intestine and kidney had a high transglucosylase activity to form a new type of glucosylated aa, which was associated with alpha-glucosidase activity. the new compound was demonstrated to be an equimolar conjugate of aa and ... | 1990 | 2200520 |
| purification and properties of nadp(+)-dependent glycerol dehydrogenases from aspergillus nidulans and a. niger. | glycerol dehydrogenase, nadp(+)-specific (ec 1.1.1.72), was purified from mycelium of aspergillus nidulans and aspergillus niger using different purification procedures. both enzymes had an mr of approximately 38,000 and were immunologically cross-reactive, but had different amino acid compositions and isoelectric points. for both enzymes, the substrate specificity was limited to glycerol and erythritol for the oxidative reaction and to dihydroxyacetone (dha), diacetyl, methylglyoxal, erythrose ... | 1990 | 2200840 |
| calcium binding in alpha-amylases: an x-ray diffraction study at 2.1-a resolution of two enzymes from aspergillus. | x-ray diffraction analysis (at 2.1-a resolution) of an acid alpha-amylase from aspergillus niger allowed a detailed description of the stereochemistry of the calcium-binding sites. the primary site (which is essential in maintaining proper folding around the active site) contains a tightly bound ca2+ with an unusually high number of eight ligands (o delta 1 and o delta 2 of asp175, o delta of asn121, main-chain carbonyl oxygens of glu162 and glu210, and three water molecules). a secondary bindin ... | 1990 | 2207069 |
| a mutation which modifies the activity of a translational suppressor in aspergillus nidulans. | a third type of translational fidelity mutation has been induced in aspergillus nidulans. the new mutation enhances growth, in suppressing conditions, of a strain containing suppressor suac109 and antisuppressor asud14 and is called alob8 for its allosuppressor activity. compared with the progenitor strain (asud14, suac109), ribosomes from the new mutant (alob8, asud14, suac109) increase misincorporation of leucine in a poly(u)-dependent homologous cell-free assay. the misreading level is mainta ... | 1990 | 2207157 |
| [pulmonary aspergilloma caused by aspergillus niger]. | the authors present a case report of a 66 year old male that was admitted due to pulmonary lesions with cavitation localized in the upper left lobe. in the cavity an abnormal mass could be seen. in the differential diagnosis malignancy and tuberculosis were excluded. fungi were cultured from the bronchial washings and sputum. their morphological characteristics allowed them to be typed as aspergillus niger. slides from the thick, mucoid, black sputum demonstrated typical forms of the fungus. pol ... | 1990 | 2216916 |
| cloning and expression of a second aspergillus niger pectin lyase gene (pela): indications of a pectin lyase gene family in a. niger. | using the previously cloned aspergillus niger n756 pectin lyase d gene as a probe, the corresponding peld gene has been isolated from a genomic library of the laboratory strain a. niger n400. this gene encodes pld, previously described as pli, which is one of the two major pectin lyases isolated from the commercial pectinase preparation ultrazym. heterologous hybridization of the a. niger n400 genomic library with the peld gene led to the isolation of another five genes: pela, b, c, e, and f. th ... | 1990 | 2225145 |
| molecular cloning, nucleotide sequence and expression of the gene encoding prepro-polygalacturonaseii of aspergillus niger. | polygalacturonaseii of aspergillus niger was fragmented using cnbr and the nh2-terminal fragment and another fragment were partially sequenced. the polygalacturonaseii (pgaii) gene was then isolated by using an oligonucleotide mixture based on the internal amino acid sequence as a probe. the nucleotide sequence of the pgaii structural gene was determined. it was found that polygalacturonaseii is synthesized as a precursor having an nh2-terminal prepro-sequence of 27 amino acids. the cloned gene ... | 1990 | 2226842 |
| characterization of a glycerol kinase mutant of aspergillus niger. | a glycerol-kinase-deficient mutant of aspergillus niger was isolated. genetic analysis revealed that the mutation is located on linkage group vi. the phenotype of this mutant differed from that of a glycerol kinase mutant of aspergillus nidulans in its ability to utilize dihydroxyacetone (dha). the weak growth on glycerol of the a. niger glycerol kinase mutant showed that glycerol phosphorylation is an important step in glycerol catabolism. the mutant could still grow normally on dha because of ... | 1990 | 2230717 |
| fungi associated with dried fish in sri lanka. | the mycoflora of dried-salted fish from markets in kandy, sri lanka was studied with emphasis on visibly spoiled dried fish. a total of 61 fungal isolates from 25 dried-fish were isolated and identified. the most prevalent fungus was aspergillus niger. species of aspergillus flavus, a. fumigatus, a. glaucus, a. restrictus, aureobasidium spp. basipetospora halophila (a genuinely halophilic fungus) cladosporium herbarum, gliomastix, spp., penicillium chalybeum and penicillium expansum were present ... | 1990 | 2233981 |
| [otomycoses in the kuznetsk region and organization of medical services for this group of population]. | in the kuzbass region the author examined 238 patients, aged 15 to 80 years, with persistent otitis or inflammatory pathologies of the ears that could not be cured by routine therapy. as a result, in 90 patients (37.8%) otomycosis was diagnosed. mycosis of the external acoustic meatus was identified in 43 patients (55 mycotic ears or 52.4%), fungal otitis media was found in 21 patients (22 ears or 20.9%), and mycosis of the postoperative cavity was detected in 26 patients (28 mycotic cavities or ... | 1990 | 2238355 |
| synthesis and pharmacological studies of some (1,4)-naphthoquinono[3,2-c]-1h-pyrazoles, 2-substituted amino-1,4-naphthoquinones, and related compounds. | series of (1,4)-naphthoquinono(3,2-c)-1h-pyrazoles and 2-substituted amino-1,4-naphthoquinones have been synthesised and studied for their possible anticancer activity (animal tumours, walker 256 carcinosarcoma), influenza rna transcriptase activity, antibiotic activity (c. neoformans, t. mentagraphytes, m. canis, a. niger, and c. albicans). | 1990 | 2241532 |
| the development of a heterologous transformation system for the cellulolytic fungus trichoderma reesei based on a pyrg-negative mutant strain. | six uridine auxotroph mutants of trichoderma reesei qm 9414 were isolated by resistance to 5-fluoroorotic acid and one strain was identified as omp-decarboxylase negative (pyr-) by a radiometric enzyme assay. transformation to uridine prototrophy was achieved with the pyr4 gene of neurospora crassa (up to 1500 transformants/micrograms) and with pyra of aspergillus niger (700-800 transformants/micrograms). in many transformants the pyr+ function seems to be present as extrachromosomal dna. there ... | 1990 | 2245476 |
| isolation and molecular characterisation of the benzoate-para-hydroxylase gene (bpha) of aspergillus niger: a member of a new gene family of the cytochrome p450 superfamily. | the gene coding for benzoate-para-hydroxylase (bpha) of aspergillus niger was cloned using differential hybridisation techniques and complementation of mutants deficient in this enzyme activity. the nucleotide sequence of the gene was determined, the presence of two introns was shown and the transcription start and termination sites were determined. the structure of the mrna upstream from the long open reading frame (orf) is unusual. it contains two small, overlapping orfs whose function is unkn ... | 1990 | 2250647 |
| seasonal and daily variation of aspergillus mich. ex fr. spores in the atmosphere of córdoba (spain). | the atmosphere contains a complex of spores whose concentration and variation have been the subject of much study, mainly because of the relevance of fungi to allergic and aerobiological pollution problems. this paper reports on the cataloguing of the aspergillus species present in the atmosphere of córdoba, their seasonal and daily variation and their potential relationship with meterological parameters. the sampling was carried out in the city of córdoba by using a volumetric trap on a culture ... | 1990 | 2251978 |
| individual patterns of immediate skin reactivity to mold extracts. | one hundred atopic patients were skin tested intradermally over a 2-year period with 30 different mold extracts. subjects were monitored for immediate reactions. data suggest that to evaluate mold sensitivity in atopic patients one must use multiple mold extracts. | 1990 | 2256573 |
| heterologous gene expression by filamentous fungi: secretion of human interleukin-6 by aspergillus nidulans. | expression vectors for human interleukin-6 (hil6) contain an expression cassette consisting of the aspergillus niger glaa promoter and the aspergillus nidulans argb terminator. the secretion signals were either those of glaa or that of the authentic hil6 peptide. the constructs under study were introduced into a. nidulans and a. niger by means of cotransformation. no il6 activity could be detected in the medium of a cotransformed a. niger strain, although transcripts corresponding with the il6 c ... | 1990 | 2258049 |
| citric acid production with mixed strains of aspergillus niger in submerged culture. | citric acid yield of 8 different strains of aspergillus niger in pure and mixed cultures was investigated. the yield varied remarkably with the strain and combination of strains. generally, the yield of citric acid for a given strain was higher in pure than in mixed culture. however, low yielding strains in combination often augmented the yield under mixed culture. the possibility of an additive genic effect is discussed. | 1990 | 2264456 |
| irreversible thermoinactivation of glucoamylase from aspergillus niger and thermostabilization by chemical modification of carboxyl groups. | the incubation of glucoamylase from aspergillus niger at 70 degrees c induced its rapid and irreversible inactivation. the covalent modifications of the protein structure involved in the thermoinactivation depended on the ph of the medium. we observed the formation of a low amount of disulfide-linked oligomers showing that disulfide exchange takes place at ph 5.5. hydrolysis of peptide bonds at ph 3.5 and 4.5 was also detected. the chemical modification of carboxyl groups with a water-soluble ca ... | 1990 | 2265196 |
| fungal flora associated with combine harvester wheat and sorghum dusts from egypt. | 107 species and 8 species varieties belonging to 44 genera were collected from combine harvester wheat and sorghum dusts (35 genera and 91 species + 4 varieties) and from the atmosphere of their hay sites (26 genera and 69 species + 4 varieties) on glucose- and cellulose-czapek's dox agar at 28 degrees c and 45 degrees c. the mycoflora of wheat and sorghum dusts were basically similar on the two types of media and the most common fungi were: alternaria alternata, aspergillus flavus, a. fumigatus ... | 1990 | 2266490 |
| genetic analysis of amds transformants of aspergillus niger and their use in chromosome mapping. | the aspergillus nidulans gene coding for acetamidase (amds) was introduced into a. niger by transformation. twelve amd+ transformants were analysed genetically. the amds inserts were located in seven different linkage groups. in each transformant the plasmid was integrated in only a single chromosome. our (non-transformed) a. niger strains do not grow on acetamide and are more resistant to fluoroacetamide than the transformants. diploids hemizygous for the amds insert have the amd+ phenotype. we ... | 1990 | 2274031 |
| expression and secretion in aspergillus nidulans and aspergillus niger of a cell surface glycoprotein from the cattle tick, boophilus microplus, by using the fungal amds promoter system. | a cell surface glycoprotein (bm86) from cells of the digestive tract of the cattle tick boophilus microplus, which has been shown to elicit a protective immunological response in vaccinated cattle, was expressed and secreted in the filamentous fungi aspergillus nidulans and aspergillus niger by using the fungal amds promoter system. the cloned gene coded for the bm86 secretory signal and all of the bm86 mature polypeptide except for the hydrophobic carboxy-terminal segment. high levels of bm86 m ... | 1990 | 2275533 |
| general and kinetic properties of endoglucanase from aspergillus niger. | endoglucanase of aspergillus niger as-101 was partially purified by ammonium sulphate fractionation and molecular sieving on sephadex g-200. the enzyme was found to be unstable on storage, however, it received some protection on the addition of bsa, glycerol or sodium azide. glycerol also protected the enzyme from inactivation due to freezing and thawing. studies on the effect of thiol group reagents revealed the involvement of -sh group(s) at the active site of enzyme. the enzyme was a metallo- ... | 1990 | 2276610 |
| an electrophoretic karyotype of aspergillus niger. | an electrophoretic karyotype of aspergillus niger was obtained using contour-clamped homogeneous electric field (chef) gel electrophoresis. chromosome-sized dna was separated into four bands. seven of the eight linkage groups could be correlated with specific chromosomal bands. for this purpose dna preparations from seven transformant strains of a. niger each carrying the heterologous amds gene of aspergillus nidulans on a different chromosome were analysed. some of the assignments were confirme ... | 1990 | 2277644 |
| isolation and molecular analysis of the orotidine-5'-phosphate decarboxylase gene (pyrg) of phycomyces blakesleeanus. | the pyrg gene of phycomyces was isolated from a phycomyces genomic library, constructed in the cosmid phs255, by hybridization with a 170 bp fragment of the pyrg gene of aspergillus niger. this fragment includes a consensus sequence found in almost all species in which the orotidine-5'-phosphate decarboxylase (ompdecase) gene has been sequenced. the complete nucleotide sequence of the cloned pyrg gene from phycomyces was determined and the transcription start sites mapped. in the predicted amino ... | 1990 | 2277645 |
| glucose oxidase as the antifungal principle of talaron from talaromyces flavus. | analysis of an authentic sample of the antifungal antibiotic talaron from the biocontrol fungus talaromyces flavus indicated that approximately 40% of the solid sample was glucose oxidase. high-performance liquid chromatography elution profiles of the antimicrobial activity of talaron coeluted with those of glucose oxidase. fluorescence emission and excitation wavelength maxima for talaron were similar to those of glucose oxidase from aspergillus niger. the molecular weight of talaron was 152,00 ... | 1990 | 2279238 |
| binding of isomaltose and maltose to the glucoamylase from aspergillus niger, as studied by fluorescence spectrophotometry and steady-state kinetics. | the binding of maltose, isomaltose, and d-glucono-1,5-lactone to the glucoamylase [e.c.3.2.1.3] from aspergillus niger was monitored by the fluorescence-intensity change (delta f) based on the tryptophan residues of the enzyme, and the binding parameters (kd and delta fmax) were evaluated from the dependence of delta f on the concentration of substrate and analogue. maltose caused the fluorescence-intensity change, but isomaltose did not, although it is hydrolyzed by the enzyme. both substrates ... | 1990 | 2279245 |
| structure and absolute configuration of pyrophen, a novel pryrone derivative of l-phenylalanine from aspergillus niger. | pyrophen, a novel 4-methoxy-2-pyrone derivative of l-phenylalanine isolated from cultures of aspergillus niger, crystallizes in the orthorhombic space group p2(1)2(1)2(1), with a = 8.918(1), b = 9.199(3), and c = 18.092(4) a. the structure was solved by direct methods and the absolute configuration assigned by the bijvoet method. refinement gave r = 0.045 and s = 1.78 for 1677 reflections with i greater than 3 sigma (i). though numerous other pyrone natural products are known, including other pr ... | 1990 | 2279852 |
| microbial metabolism studies of the antimalarial drug arteether. | microbial metabolism studies of the antimalarial drug arteether (1) have shown that arteether is metabolized by a number of microorganisms. large-scale fermentation with aspergillus niger (atcc 10549) and nocardia corallina (atcc 19070) have resulted in the isolation of four microbial metabolites which have been characterized using two-dimensional nuclear magnetic resonance (2d-nmr) techniques. these metabolites have been identified as "aem1" (2), 3 alpha-hydroxydeoxyarteether (3), 3 alpha-hydro ... | 1990 | 2308900 |
| new pathway for the biodegradation of indole in aspergillus niger. | indole and its derivatives form a class of toxic recalcitrant environmental pollutants. the growth of aspergillus niger was inhibited by very low concentrations (0.005 to 0.02%) of indole, even when 125- to 500-fold excess glucose was present in the medium. when 0.02% indole was added, the fungus showed a lag phase for about 30 h and the uptake of glucose was inhibited. indole was metabolized by a new pathway via indoxyl (3-hydroxyindole), n-formylanthranilic acid, anthranilic acid, 2,3-dihydrox ... | 1990 | 2310183 |
| airway obstruction caused by aspergillus tracheobronchitis in an immunocompromised patient. | | 1990 | 2328603 |
| purification and characterization of polygalacturonases produced by the hyphal fungus aspergillus niger. | five endo-polygalacturonases (poly(1,4-alpha-d-galacturonide) glycanohydrolase, ec 3.2.1.15) and one exo-polygalacturonase (poly(1,4-alpha-d-galacturonide) galacturonohydrolase, ec 3.2.1.67) were isolated from a commercial pectinase preparation derived from aspergillus niger. all five endo-enzymes could be purified to homogeneity by affinity chromatography on cross-linked alginate, ion-exchange chromatography, chromatofocusing, and gel permeation chromatography. the exo-polygalacturonase was onl ... | 1990 | 2331322 |
| lentiginosine, a dihydroxyindolizidine alkaloid that inhibits amyloglucosidase. | lentiginosine, a dihydroxyindolizidine alkaloid, was extracted from the leaves of astragalus lentiginosus with hot methanol and was purified to homogeneity by ion-exchange, thin-layer, and radial chromatography. a second dihydroxyindolizidine, the 2-epimer of lentiginosine, was also purified to apparent homogeneity from these extracts. gas chromatography of the two isomers (as the tms derivatives) showed that they were better than 95% pure; lentiginosine eluted at 8.65 min and the 2-epimer at 9. ... | 1990 | 2331469 |
| cloning, characterization, and expression of two alpha-amylase genes from aspergillus niger var. awamori. | using synthetic oligonucleotide probes, we cloned genomic dna sequences encoding an alpha-amylase gene from aspergillus niger var. awamori (a. awamori) on a 5.8 kb ecori fragment. hybridization experiments, using a portion of this cloned fragment to probe dna from a. awamori, suggested the presence of two alpha-amylase gene copies which were subsequently cloned as 7 kb (designated as amya) and 4 kb (amyb) hindiii fragments. dna sequence analysis of the amya and amyb genes revealed the following: ... | 1990 | 2340591 |
| crystallization and preliminary x-ray diffraction studies of a deglycosylated glucose oxidase from aspergillus niger. | deglycosylation was shown to be an important prerequisite step for the crystallization of glucose oxidase from aspergillus niger. whereas the glycosylated enzyme could not be crystallized, crystals of the deglycosylated enzyme suitable for x-ray diffraction analysis were reproducibly grown in the presence of 1.6 m-ammonium sulphate and octanetriol at ph 5.3 to 5.6. the crystals belong to the space group p3(1)21 or p3(2)21 with refined lattice constants of a = 66.5 a and c = 214.4 a, indicating a ... | 1990 | 2342102 |
| studies on the mycoflora of aswan high dam lake, egypt: monthly variations. | fifty-one species and one variety appertaining to twenty one genera of mesophilic fungi were recovered from the monthly samples of marginal water (44 species, 1 variety and 18 genera) and submerged mud (78 species, 1 variety and 30 genera) of aswan high dam lake during the period from july 1985 to december 1986. the most common species were aspergillus fumigatus, a. flavus, a. terreus, a. niger and penicillium funiculosum. the highest fungal populations were almost detected either in october, in ... | 1990 | 2352135 |
| [distribution studies of alpha-l-arabinofuranosidase already unstable at ph 7.0 (37 degrees c) in tumor-bearing mice in connection with its possible use to enhance the selectivity of the chemotherapy of malignant tumors]. | graffi et al. (1-3) had proposed the use of exogenous enzymes to toxify inactive transport forms of cancerostatic substances. for this purpose, the ph difference between normal tissues and the tumor was to be exploited, which can be essentially increased by the application of glucose and inorganic phosphate (5-7). earlier studies using alpha-l-arabinofuranosidase obtained from aspergillus niger have shown that the selectivity of tumor chemotherapy can be increased in this way (4). the alpha-l-ar ... | 1990 | 2369282 |
| isolation and structure of the pectin lyase d-encoding gene from aspergillus niger. | the filamentous fungus, aspergillus niger, produces a number of extracellular pectin-degrading enzymes. we present here the isolation and the complete nucleotide sequence of the gene, peld, coding for a pectin lyase d (pld), which was previously described as pectin lyase i (van houdenhoven, ph.d. thesis, wageningen, 1975). the deduced amino acid (aa) sequence corresponds to 373 aa residues including a signal peptide of 19 aa. the coding region is interrupted by four short introns (57-65 bp). the ... | 1990 | 2373363 |
| nucleotide and derived amino acid sequence of a pectinesterase cdna isolated from aspergillus niger strain rh 5344. | | 1990 | 2377474 |
| protoplast fusion in aspergillus niger strains accumulating citric acid. | auxotrophic strains of aspergillus niger were obtained from citric-acid-producing strains of the fungus after irradiation with uv light. protoplasts were isolated from young hyphae of the auxotrophic strains after treatment with snail enzyme and than treated with polyethylene glycol (30%, w/v), in a ca2+ (10 mmol/l) solution. the ph value of the suspension was adjusted to 9.0. the frequency of the heterokaryons (related to the number of protoplasts reverting after peg treatment) was 0.67%. proto ... | 1990 | 2379887 |
| genetic analysis of aspergillus niger: isolation of chlorate resistance mutants, their use in mitotic mapping and evidence for an eighth linkage group. | this paper describes the use of chlorate resistant mutants in genetic analysis of aspergillus niger. the isolated mutants could be divided into three phenotypic classes on the basis of nitrogen utilization. these were designated nia, nir and cnx as for aspergillus nidulans. all mutations were recessive to their wild-type allele in heterokaryons as well as in heterozygous diploids. the mutations belong to nine different complementation groups. in addition a complex overlapping complementation gro ... | 1990 | 2381424 |
| localization of pyruvate carboxylase in organic acid-producing aspergillus strains. | the localization of pyruvate carboxylase (cytosolic or mitochondrial) was studied in nine different aspergillus species (14 strains). in some species (a. aculeatus, a. flavus, a. foetidus, a. nidulans, a. ochraceus, and a. sojae), the pyruvate carboxylase activity could be detected only in the cytosolic fraction of the cells. pyruvate carboxylase has been found only in the mitochondrial fraction of two strains of aspergillus wentii. in aspergillus oryzae and in five strains of aspergillus niger, ... | 1990 | 2383004 |
| occurrence of zoosporic and terrestrial fungi in some ponds of kharga oases, egypt. | thirty-nine species belonging to 13 genera of zoosporic fungi were collected from 33 water samples on sesame and hemp seeds as baits at 22 degrees c. five genera were isolated in high occurrence: allomyces (2 species), achlya (9 species), dictyuchus (4 species), pythium (5 species) and saprolegnia (5 species). aphanomyces (4 species) and isoachlya (2 species) were of moderate frequencies. the remaining six genera were less frequent: anisolpidium, brevilegnia, calyptralegnia, leptolegnia, pythiop ... | 1990 | 2384873 |
| survey of the mycoflora and mycotoxins of cotton seeds and cotton seed products in egypt. | thirty-nine species and 16 fungal genera were isolated from egyptian cotton seeds, cotton seed meal and cotton seed cake on 1% glucose-czapek's agar medium incubated at 28 degrees c. aspergillus was the most frequent genus and it emerged in 87-100% of the samples contributing 70-98% of total fungi in the three substrates tested. the most common species were a. niger, a. flavus, a. fumigatus, a. terreus and rhizopus stolonifer; a. niger, a. fumigatus and penicillium corylophilum; and a. niger, a. ... | 1990 | 2388680 |
| mould growth on black table olives and prevention by sorbic acid, methyl-eugenol and spice essential oil. | thirty-two mould strains were isolated from turkish-style black table olives. four of these isolates were identified as aspergillus ochraceus, two as a. niger, 16 as penicillium clavigerum and 10 as p. expansum. mould growth was evaluated on olives treated with 500 ppm sorbic acid, 1000 ppm methyl-eugenol and 1500 ppm çörtük spice (echinophora sibthorpiana guss.) essential oil. olives inoculated with a. parasiticus nrrl 2999 and p. clavigerum were also examined. all samples were stored at 28 deg ... | 1990 | 2388689 |
| [comparison of bacteriostatic ability of oleum of perilla frutescens (l.) britt., cinnamomum cassia presl and nipagin a]. | a comparison of bacteriostatic ability has been carried out among oleum of perilla frutescens and cinnamomum cassia, benzoic acid and nipagin a. the result shows that the inhibiting effect of perilla frutescens and cinnamomum cassia on microorganisms inoculated and spontaneously contaminated is similar to or obviously superior to nipagin a and benzoic acid. | 1990 | 2390180 |
| isolation of fungi from local bread of chhindwara. | in the present paper, fungi isolated from bread samples are being reported. out of 150 samples of bread, only 20 samples appeared to be infected but when cultured all the samples could not give positive results, except, a. fumigatus. ten fungi including aspergillus fumigatus, a. niger, a. flavus, a. glaucous, a. carnous, a. terreus, a. ochraceous, rhizophus stolonifer, trichoderma harrianua, and trichothecium roseum were recovered from the bread samples. among these a. fumigatus and a. niger wer ... | 1990 | 2391152 |
| complete genomic sequence encoding trpc from aspergillus niger var. awamori. | | 1990 | 2395660 |
| cloning and dna sequence analysis of a polygalacturonase cdna from aspergillus niger rh5344. | a 1319 bp long cdna encoding for a polygalacturonase (ec 3.2.1.15) from aspergillus niger rh5344 comprises a single open reading frame of 1089 bp which includes the mature protein of 362 amino acids and an nh2-terminal signal peptide of 27 amino acids. the directly determined peptides of the mature polygalacturonase confirmed the sequence information deduced from the cdna. | 1990 | 2400785 |
| common amino acid domain among endopolygalacturonases of ascomycete fungi. | the endopolygalacturonase (ec 3.2.1.15) enzymes produced in vitro by three ascomycete fungi, aspergillus niger, sclerotinia sclerotiorum, and colletotrichum lindemuthianum were studied by using thin-layer isoelectric focusing and activity stain overlay techniques. the polygalacturonases from a. niger and s. sclerotiorum consisted of numerous isoforms, whereas the endopolygalacturonase from c. lindemuthianum consisted of a single protein species. the most abundant endopolygalacturonase isoform pr ... | 1990 | 2403258 |