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a tree of life based on ninety-eight expressed genes conserved across diverse eukaryotic species.rapid advances in dna sequencing technologies have resulted in the accumulation of large data sets in the public domain, facilitating comparative studies to provide novel insights into the evolution of life. phylogenetic studies across the eukaryotic taxa have been reported but on the basis of a limited number of genes. here we present a genome-wide analysis across different plant, fungal, protist, and animal species, with reference to the 36,002 expressed genes of the rice genome. our analysis ...201728922368
carbon-doped mesoporous anatase tio2 multi-tubes nanostructures for highly improved visible light photocatalytic activity.development of a high surface area and efficient visible light induced photocatalyst on a large scale is a promising task from the practical perspective. in this study, visible light active c-doped anatase tio2 multi-tubes were synthesized using banana (musa acuminata) stem fiber as a sacrificial template, removed by calcination at 450 °c. during the calcination process, the lattice of anatase tio2 phase was doped with c, and obtained multi-tubes showed high surface area (∼99 m2/g) with a mesopo ...201728783348
the agpase family proteins in banana: genome-wide identification, phylogeny, and expression analyses reveal their involvement in the development, ripening, and abiotic/biotic stress responses.adp-glucose pyrophosphorylase (agpase) is the first rate-limiting enzyme in starch biosynthesis and plays crucial roles in multiple biological processes. despite its importance, agpase is poorly studied in starchy fruit crop banana (musa acuminata l.). in this study, eight maagpase genes have been identified genome-wide in m. acuminata, which could be clustered into the large (apl) and small (aps) subunits. comprehensive transcriptomic analysis revealed temporal and spatial expression variations ...201728757545
genome-wide analysis of banana mads-box family closely related to fruit development and ripening.proteins encoded by mads-box genes are important transcription factors involved in the regulation of flowering plant growth and development. currently, no systematic information exists regarding the mads-box family in the important tropical fruit banana. ninety-six mads-box genes were identified from the banana (pahang) a genome. phylogenetic analysis indicated that musa acuminata mcm1-agamous- deficiens-srf (mamads) could be divided into mikcc, mikc*, mα/β and mγ groups. mikcc could be further ...201728615681
evolution of the banana genome (musa acuminata) is impacted by large chromosomal translocations.most banana cultivars are triploid seedless parthenocarpic clones derived from hybridization between musa acuminata subspecies and sometimes m. balbisiana. m. acuminata subspecies were suggested to differ by a few large chromosomal rearrangements based on chromosome pairing configurations in intersubspecies hybrids. we searched for large chromosomal rearrangements in a seedy m. acuminata ssp. malaccensis banana accession through mate-pair sequencing, bac-fish, targeted pcr and marker (dartseq) s ...201728575404
soluble starch synthase iii-1 in amylopectin metabolism of banana fruit: characterization, expression, enzyme activity, and functional analyses.soluble starch synthase (ss) is one of the key enzymes involved in amylopectin biosynthesis in plants. however, no information is currently available about this gene family in the important fruit crop banana. herein, we characterized the function of massiii-1 in amylopectin metabolism of banana fruit and described the putative role of the other mass family members. firstly, starch granules, starch and amylopectin content were found to increase during banana fruit development, but decline during ...201728424724
the banana (musa acuminata) genome and the evolution of monocotyledonous plants.bananas (musa spp.), including dessert and cooking types, are giant perennial monocotyledonous herbs of the order zingiberales, a sister group to the well-studied poales, which include cereals. bananas are vital for food security in many tropical and subtropical countries and the most popular fruit in industrialized countries. the musa domestication process started some 7,000 years ago in southeast asia. it involved hybridizations between diverse species and subspecies, fostered by human migrati ...022801500
transcriptome sequencing of mycosphaerella fijiensis during association with musa acuminata reveals candidate pathogenicity genes.mycosphaerella fijiensis, causative agent of the black sigatoka disease of banana, is considered the most economically damaging banana disease. despite its importance, the genetics of pathogenicity are poorly understood. previous studies have characterized polyketide pathways with possible roles in pathogenicity. to identify additional candidate pathogenicity genes, we compared the transcriptome of this fungus during the necrotrophic phase of infection with that during saprophytic growth in medi ...201627576702
efficient transformation of mycosphaerella fijiensis by underwater shock waves.black leaf streak disease, also known as black sigatoka, causes dramatic losses in production of banana and plantains fruits. the disease is caused by the pathogenic fungus mycosphaerella fijiensis (anamorph pseudocercospora fijiensis; mycosphaerellaceae). genetic transformation of m. fijiensis would allow a better understanding of molecular basis of pathogenicity and design novel approaches to control the infection caused by this pathogen. however, transformation of this fungus has not been eas ...201526456044
effect of thai banana (musa aa group) in reducing accumulation of oxidation end products in uvb-irradiated mouse skin.chronic uvb exposure causes skin disorders and cancer through dna strand breaks and oxidation of numerous functional groups of proteins and lipids in the skin. in this study, we investigated the effects of thai banana (musa aa group, "khai," and musa abb group, "namwa") on the prevention of uvb-induced skin damage when fed to male icr mice.201728187402
phlebotomine sandfly ecology on the indian subcontinent: does village vegetation play a role in sandfly distribution in bihar, india?visceral leishmaniasis (vl) is a disease that results in approximately 50 000 human deaths annually. it is transmitted through the bites of phlebotomine sandflies and around two-thirds of cases occur on the indian subcontinent. indoor residual spraying (irs), the efficacy of which depends upon sandfly adults resting indoors, is the only sandfly control method used in india. recently, in bihar, india, considerable sandfly numbers have been recorded outdoors in village vegetation, which suggests t ...201728106262
the effect of blue light on stomatal oscillations and leaf turgor pressure in banana leaves.stomatal oscillations are cyclic opening and closing of stomata, presumed to initiate from hydraulic mismatch between leaf water supply and transpiration rate. to test this assumption, mismatches between water supply and transpiration were induced using manipulations of vapour pressure deficit (vpd) and light spectrum in banana (musa acuminata). simultaneous measurements of gas exchange with changes in leaf turgor pressure were used to describe the hydraulic mismatches. an increase of vpd above ...201728098339
banana mamads transcription factors are necessary for fruit ripening and molecular tools to promote shelf-life and food security.genetic solutions to postharvest crop loss can reduce cost and energy inputs while increasing food security, especially for banana (musa acuminata), which is a significant component of worldwide food commerce. we have functionally characterized two banana e class (sepallata3 [sep3]) mads box genes, mamads1 and mamads2, homologous to the tomato (solanum lycopersicum) rin-mads ripening gene. transgenic banana plants repressing either gene (via antisense or rna interference [rnai]) were created and ...201626956665
revisiting the zingiberales: using multiplexed exon capture to resolve ancient and recent phylogenetic splits in a charismatic plant lineage.the zingiberales are an iconic order of monocotyledonous plants comprising eight families with distinctive and diverse floral morphologies and representing an important ecological element of tropical and subtropical forests. while the eight families are demonstrated to be monophyletic, phylogenetic relationships among these families remain unresolved. neither combined morphological and molecular studies nor recent attempts to resolve family relationships using sequence data from whole plastomes ...201626819846
the transcriptional regulatory network mediated by banana (musa acuminata) dehydration-responsive element binding (madreb) transcription factors in fruit ripening.fruit ripening is a complex, genetically programmed process involving the action of critical transcription factors (tfs). despite the established significance of dehydration-responsive element binding (dreb) tfs in plant abiotic stress responses, the involvement of drebs in fruit ripening is yet to be determined. here, we identified four genes encoding ripening-regulated dreb tfs in banana (musa acuminata), madreb1, madreb2, madreb3, and madreb4, and demonstrated that they play regulatory roles ...201728044313
use of banana (musa acuminata colla aaa) peel extract as an antioxidant source in orange juices.using banana peel extract as an antioxidant in freshly squeezed orange juices and juices from concentrate was evaluated. free radical scavenging capacity increased by adding banana peel extracts to both types of orange juice. in addition, remarkable increases in antioxidant capacity using 2,2'-azino-bis-(3-ethylbenzothiazoline)-6-sulfonic acid (abts) radical were observed when equal or greater than 5 mg of banana peel extract per ml of freshly squeezed juice was added. no clear effects were obse ...201727987069
antioxidant and antihyperglycemic properties of three banana cultivars (musa spp.).background. this study sought to investigate the antioxidant and antihyperglycemic properties of musa sapientum (latundan banana) (msl), musa acuminata (cavendish banana) (mac), and musa acuminate (red dacca) (mar). materials and methods. the sugar, starch, amylose, and amylopectin contents and glycemic index (gi) of the three banana cultivars were determined. furthermore, total phenol and vitamin c contents and α-amylase and α-glucosidase inhibitory effects of banana samples were also determine ...201627872791
employment of proteomic and immunological based methods for the identification of catalase as novel allergen from banana.diagnostic reagents based on food allergen extracts often lack sufficient sensitivity. the introduction of well characterized food allergens in molecular allergy diagnosis has been recognized as valid approach to circumvent unstandardized allergen extracts. banana fruit (musa acuminata) is a well-established allergen source which besides six characterized allergens, contains unidentified ige reactive proteins whose clinical relevance remains undefined. by employment of a combinatorial peptide li ...201829331514
further insights into the metal ion binding abilities and the metalation pathway of a plant metallothionein from musa acuminata.the superfamily of metallothioneins (mts) combines a diverse group of metalloproteins, sharing the characteristics of rather low molecular weight and high cysteine content. the latter provides mts with the capability to coordinate thiophilic metal ions, in particular those with a d 10 electron configuration. the sub-family of plant mt3 proteins is only poorly characterized and there is a complete lack of three-dimensional structure information. building upon our previous results on the musa acum ...201829218632
long noncoding rnas that respond to fusarium oxysporum infection in 'cavendish' banana (musa acuminata).long noncoding rnas (lncrnas) are a class of genes that influence a variety of biological functions through acting as signal, decoy, guide, and scaffold molecules. in banana (musa spp.), an important economic fruit crop, particularly in southeast asia, the wilt disease caused by fusarium oxysporum f. sp. cubense (foc), especially strain foc tr4, is disastrous. in banana, how the biogenesis of these lncrnas is regulated in response to pathogen infection is still largely unknown. in this study, st ...201729209086
characterization and amplification of gene-based simple sequence repeat (ssr) markers in date palm.the paucity of molecular markers limits the application of genetic and genomic research in date palm (phoenix dactylifera l.). availability of expressed sequence tag (est) sequences in date palm may provide a good resource for developing gene-based markers. this study characterizes a substantial fraction of transcriptome sequences containing simple sequence repeats (ssrs) from the est sequences in date palm. the est sequences studied are mainly homologous to those of elaeis guineensis and musa a ...201728755229
dietary intervention with green dwarf banana flour (musa sp aaa) prevents intestinal inflammation in a trinitrobenzenesulfonic acid model of rat colitis.dietary products are among the therapeutic approaches used to modify intestinal microflora and to promote protective effects during the intestinal inflammatory process. because the banana plant is rich in resistant starch, which is used by colonic microbiota for the anaerobic production of the short-chain fatty acids that serve as a major fuel source for colonocytes: first, green dwarf banana flour produces protective effects on the intestinal inflammation acting as a prebiotic and, second, comb ...201222464807
tissue cell assisted fabrication of tubular catalytic platinum microengines.we report a facile platform for mass production of robust self-propelled tubular microengines. tissue cells extracted from fruits of banana and apple, musa acuminata and malus domestica, are used as the support on which a thin platinum film is deposited by means of physical vapor deposition. upon sonication of the cells/pt-coated substrate in water, microscrolls of highly uniform sizes are spontaneously formed. tubular microengines fabricated with the fruit cell assisted method exhibit a fast mo ...201425143056
analysis of the leaf transcriptome of musa acuminata during interaction with mycosphaerella musicola: gene assembly, annotation and marker development.although banana (musa sp.) is an important edible crop, contributing towards poverty alleviation and food security, limited transcriptome datasets are available for use in accelerated molecular-based breeding in this genus. 454 gs-flx titanium technology was employed to determine the sequence of gene transcripts in genotypes of musa acuminata ssp. burmannicoides calcutta 4 and m. acuminata subgroup cavendish cv. grande naine, contrasting in resistance to the fungal pathogen mycosphaerella musico ...201323379821
translating the "banana genome" to delineate stress resistance, dwarfing, parthenocarpy and mechanisms of fruit ripening.evolutionary frozen, genetically sterile and globally iconic fruit "banana" remained untouched by the green revolution and, as of today, researchers face intrinsic impediments for its varietal improvement. recently, this wonder crop entered the genomics era with decoding of structural genome of double haploid pahang (aa genome constitution) genotype of musa acuminata. its complex genome decoded by hybrid sequencing strategies revealed panoply of genes and transcription factors involved in the pr ...201627833619
hormonal and hydroxycinnamic acids profiles in banana leaves in response to various periods of water stress.the pattern of change in the endogenous levels of several plant hormones and hydroxycinnamic acids in addition to growth and photosynthetic performance was investigated in banana plants (musa acuminata cv. "grand nain") subjected to various cycles of drought. water stress was imposed by withholding irrigation for six periods with subsequent rehydration. data showed an increase in abscisic acid (aba) and indole-3-acetic acid (iaa) levels, a transient increase in salicylic acid (sa) concentration, ...201424977208
genome-wide analysis of the dna-binding with one zinc finger (dof) transcription factor family in bananas.dna-binding with one finger (dof) domain proteins are a multigene family of plant-specific transcription factors involved in numerous aspects of plant growth and development. in this study, we report a genome-wide search for musa acuminata dof (madof) genes and their expression profiles at different developmental stages and in response to various abiotic stresses. in addition, a complete overview of the dof gene family in bananas is presented, including the gene structures, chromosomal locations ...201627831816
dart whole genome profiling provides insights on the evolution and taxonomy of edible banana (musa spp.).dessert and cooking bananas are vegetatively propagated crops of great importance for both the subsistence and the livelihood of people in developing countries. a wide diversity of diploid and triploid cultivars including aa, ab, as, at, aaa, aab, abb, aas and aat genomic constitutions exists. within each of this genome groups, cultivars are classified into subgroups that are reported to correspond to varieties clonally derived from each other after a single sexual event. the number of those fou ...201627590334
phenylphenalenones accumulate in plant tissues of two banana cultivars in response to herbivory by the banana weevil and banana stem weevil.phenylphenalenone-type compounds accumulated in the tissues of two banana cultivars-musa acuminata cv. "grande naine" (aaa) and musa acuminata × balbisiana colla cv. "bluggoe" (abb)-when these were fed on by the banana weevil (cosmopolites sordidus (germ.) (coleoptera: curculionidae)) and the banana stem weevil (odoiporus longicollis (oliver) (coleoptera: curculionidae)). the chemical constituents of the banana material were separated by means of chromatographic techniques and identified by nmr ...201627571112
genome-wide expression analysis and metabolite profiling elucidate transcriptional regulation of flavonoid biosynthesis and modulation under abiotic stresses in banana.flavonoid biosynthesis is largely regulated at the transcriptional level due to the modulated expression of genes related to the phenylpropanoid pathway in plants. although accumulation of different flavonoids has been reported in banana, a staple fruit crop, no detailed information is available on regulation of the biosynthesis in this important plant. we carried out genome-wide analysis of banana (musa acuminata, aaa genome) and identified 28 genes belonging to 9 gene families associated with ...201627539368
whole genome sequencing of a banana wild relative musa itinerans provides insights into lineage-specific diversification of the musa genus.crop wild relatives are valuable resources for future genetic improvement. here, we report the de novo genome assembly of musa itinerans, a disease-resistant wild banana relative in subtropical china. the assembled genome size was 462.1 mb, covering 75.2% of the genome (615.2mb) and containing 32, 456 predicted protein-coding genes. since the approximate divergence around 5.8 million years ago, the genomes of musa itinerans and musa acuminata have shown conserved collinearity. gene family expans ...201627531320
the banana transcriptional repressor madear1 negatively regulates cell wall-modifying genes involved in fruit ripening.ethylene plays an essential role in many biological processes including fruit ripening via modulation of ethylene signaling pathway. ethylene response factors (erfs) are key transcription factors (tfs) involved in ethylene perception and are divided into ap2, rav, erf, and dreb sub-families. although a number of studies have implicated the involvement of dreb sub-family genes in stress responses, little is known about their roles in fruit ripening. in this study, we identified a dreb tf with a e ...201627462342
effects of particle size distribution on some physical, chemical and functional properties of unripe banana flour.the objective of this study was to examine the effect of particle size distribution on physical, chemical and functional properties of unripe banana flour for the first time. a pure triploid (aaa group) of musa acuminata subgroup cavendish (°brix;0.2, ph;4.73, titratable acidity; 0.56g/100g malic acid, total solids; 27.42%) which was supplied from gazipaşa, antalya, turkey from october 2014 to october 2015 was used. size fractions of <212, 212-315, 316-500 and 501-700μm were characterized for th ...201627451170
molecular characterization of constans-like (col) genes in banana (musa acuminata l. aaa group, cv. grand nain).the constans (co) family is an important regulator of flowering in photoperiod sensitive plants. but information regarding their role in day neutral plants is limited. we report identification of nine group i type constans-like (col) genes of banana and their characterization for their age dependent, diurnal and tissue-specific expression. our studies show that the group i genes are conserved in structure to members in other plants. expression of these genes shows a distinct circadian regulation ...201627186015
molecular characterization of maccs, a novel copper chaperone gene involved in abiotic and hormonal stress responses in musa acuminata cv. tianbaojiao.copper/zinc superoxide dismutases (cu/znsods) play important roles in improving banana resistance to adverse conditions, but their activities depend on the copper chaperone for superoxide dismutase (ccs) delivering copper to them. however, little is known about ccs in monocots and under stress conditions. here, a novel ccs gene (maccs) was obtained from a banana using reverse transcription pcr and rapid-amplification of cdna ends (race) pcr. sequence analyses showed that maccs has typical ccs do ...201627023517
genome-wide analysis of the musa wrky gene family: evolution and differential expression during development and stress.the wrky gene family plays an important role in the development and stress responses in plants. as information is not available on the wrky gene family in musa species, genome-wide analysis has been carried out in this study using available genomic information from two species, musa acuminata and musa balbisiana. analysis identified 147 and 132 members of the wrky gene family in m. acuminata and m. balbisiana, respectively. evolutionary analysis suggests that the wrky gene family expanded much b ...201627014321
improvement of the banana "musa acuminata" reference sequence using ngs data and semi-automated bioinformatics methods.recent advances in genomics indicate functional significance of a majority of genome sequences and their long range interactions. as a detailed examination of genome organization and function requires very high quality genome sequence, the objective of this study was to improve reference genome assembly of banana (musa acuminata).201626984673
traditional banana diversity in oceania: an endangered heritage.this study aims to understand the genetic diversity of traditional oceanian starchy bananas in order to propose an efficient conservation strategy for these endangered varieties. ssr and dart molecular markers are used to characterize a large sample of pacific accessions, from new guinea to tahiti and hawaii. all pacific starchy bananas are shown of new guinea origin, by interspecific hybridization between musa acuminata (aa genome), more precisely its local subspecies m. acuminata ssp. banksii, ...201626982801
transcriptomic changes of drought-tolerant and sensitive banana cultivars exposed to drought stress.in banana, drought responsive gene expression profiles of drought-tolerant and sensitive genotypes remain largely unexplored. in this research, the transcriptome of drought-tolerant banana cultivar (saba, abb genome) and sensitive cultivar (grand naine, aaa genome) was monitored using mrna-seq under control and drought stress condition. a total of 162.36 million reads from tolerant and 126.58 million reads from sensitive libraries were produced and mapped onto the musa acuminata genome sequence ...201627867388
effect of physiological harvest stages on the composition of bioactive compounds in cavendish bananas.the combined influence of maturation, ripening, and climate on the profile of bioactive compounds was studied in banana (musa acuminata, aaa, cavendish, cv. grande naine). their bioactive compounds were determined by the folin-ciocalteu assay and high-performance thin layer chromatographic (hptlc) method. the polyphenol content of bananas harvested after 400 degree days remained unchanged during ripening, while bananas harvested after 600 and 900 degree days exhibited a significant polyphenol in ...201323549844
inhibition of the recombinant cattle tick rhipicephalus (boophilus) annulatus glutathione s-transferase.rhipicephalus (boophilus) annulatus is a bloodsucking ectoparasite that causes severe production losses in the cattle industry. this study aims to evaluate the in vitro effects of tannic acid, hematin (gst inhibitors) and different plant extracts (rich in tannic acid) on the activity of the recombinant glutathione s-transferase enzyme of the egyptian cattle tick r. annulatus (rragst), in order to confirm their ability to inhibit the parasitic essential detoxification enzyme glutathione s-transfe ...201424953796
genome-wide analysis of repeat diversity across the family musaceae.the banana family (musaceae) includes genetically a diverse group of species and their diploid and polyploid hybrids that are widely cultivated in the tropics. in spite of their socio-economic importance, the knowledge of musaceae genomes is basically limited to draft genome assemblies of two species, musa acuminata and m. balbisiana. here we aimed to complement this information by analyzing repetitive genome fractions of six species selected to represent various phylogenetic groups within the f ...201424932725
protocol for simultaneous isolation of three important banana allergens.banana fruit (musa acuminata) has become an important food allergen source in recent years. so far, 5 ige reactive banana proteins have been identified, and the major allergens are: mus a 2 (a class i chitinase, 31kda), mus a 4 (thaumatin-like protein, 21kda), and mus a 5 (β-1,3-glucanase, 33kda). due to variations in allergen expression levels, diagnostic reagents for food allergy can be improved by using individual allergen components instead of banana allergen extracts. the purpose of this st ...201424887593
lipophilic phytochemicals from banana fruits of several musa species.the chemical composition of the lipophilic extract of ripe pulp of banana fruit from several banana cultivars belonging to the musa acuminata and musa balbisiana species (namely 'chinese cavendish', 'giant cavendish', 'dwarf red', 'grand nain', 'eilon', 'gruesa', 'silver', 'ricasa', 'williams' and 'zelig') was studied by gas chromatography-mass spectrometry for the first time. the banana cultivars showed similar amounts of lipophilic extractives (ca. 0.4% of dry material weight) as well as quali ...201424874383
expansion of banana (musa acuminata) gene families involved in ethylene biosynthesis and signalling after lineage-specific whole-genome duplications.whole-genome duplications (wgds) are widespread in plants, and three lineage-specific wgds occurred in the banana (musa acuminata) genome. here, we analysed the impact of wgds on the evolution of banana gene families involved in ethylene biosynthesis and signalling, a key pathway for banana fruit ripening. banana ethylene pathway genes were identified using comparative genomics approaches and their duplication modes and expression profiles were analysed. seven out of 10 banana ethylene gene fami ...201424716518
in vitro propagation and assessment of the genetic fidelity of musa acuminata (aaa) cv. vaibalhla derived from immature male flowers.an efficient in vitro propagation method has been developed for the first time for musa acuminata (aaa) cv. vaibalhla, an economically important banana cultivar of mizoram, india. immature male flowers were used as explants. murashige and skoog's (ms) medium supplemented with plant growth regulators (pgrs) were used for the regeneration process. out of different pgr combinations, ms medium supplemented with 2 mg l(-1) 6-benzylaminopurine (bap) + 0.5 mg l(-1) α-naphthalene acetic acid (naa) was o ...201424222501
exploring wild genetic resources of musa acuminata colla distributed in the humid forests of southern western ghats of peninsular india using issr markers.musa acuminata ssp. burmannica, one of the wild progenitors contributing 'a genome' to the present-day dessert bananas, has a long evolutionary history intervened by human activities. in this study, issr markers were used to analyze the pattern of genetic variation and differentiation in 32 individuals along with two reference samples (viz., musa acuminata ssp. burmannicoides, var. calcutta 4 and musa balbisiana) of wild musa, which corresponded to three populations across the biodiversity-rich ...201222562780
"a draft musa balbisiana genome sequence for molecular genetics in polyploid, inter- and intra-specific musa hybrids".modern banana cultivars are primarily interspecific triploid hybrids of two species, musa acuminata and musa balbisiana, which respectively contribute the a- and b-genomes. the m. balbisiana genome has been associated with improved vigour and tolerance to biotic and abiotic stresses and is thus a target for musa breeding programs. however, while a reference m. acuminata genome has recently been released (nature 488:213-217, 2012), little sequence data is available for the corresponding b-genome. ...201324094114
from crossbreeding to biotechnology-facilitated improvement of banana and plantain.the annual harvest of banana and plantain (musa spp.) is approximately 145 million tons worldwide. about 85% of this global production comes from small plots and kitchen or backyard gardens from the developing world, and only 15% goes to the export trade. musa acuminata and musa balbisiana are the ancestors of several hundreds of parthenocarpic musa diploid and polyploid cultivars, which show multiple origins through inter- and intra-specific hybridizations from these two wild diploid species. g ...201424091289
annotation of differentially expressed genes in the somatic embryogenesis of musa and their location in the banana genome.analysis of cdna-aflp was used to study the genes expressed in zygotic and somatic embryogenesis of musa acuminata colla ssp. malaccensis, and a comparison was made between their differential transcribed fragments (tdfs) and the sequenced genome of the double haploid- (dh-) pahang of the malaccensis subspecies that is available in the network. a total of 253 transcript-derived fragments (tdfs) were detected with apparent size of 100-4000 bp using 5 pairs of aflp primers, of which 21 were differe ...201324027442
chlorophyll breakdown in senescent banana leaves: catabolism reprogrammed for biosynthesis of persistent blue fluorescent tetrapyrroles.chlorophyll breakdown is a visual phenomenon of leaf senescence and fruit ripening. it leads to the formation of colorless chlorophyll catabolites, a group of (chlorophyll-derived bilin-type) linear tetrapyrroles. here, analysis and structure elucidation of the chlorophyll breakdown products in leaves of banana (musa acuminata) is reported. in senescent leaves of this monocot all chlorophyll catabolites identified were hypermodified fluorescent chlorophyll catabolites (hmfccs). surprisingly, non ...201323946204
homoeologous chromosome pairing between the a and b genomes of musa spp. revealed by genomic in situ hybridization.most cooking banana and several desert bananas are interspecific triploid hybrids between musa acuminata (a genome) and musa balbisiana (b genome). in addition, m. balbisiana has agronomical characteristics such as resistance to biotic and abiotic stresses that could be useful to improve monospecific acuminata cultivars. to develop efficient breeding strategies for improving musa cultivars, it is therefore important to understand the possibility of chromosome exchange between these two species.201121835815
chemical composition and nutritional value of unripe banana flour (musa acuminata, var. nanicão).banana flour obtained from unripe banana (musa acuminata, var. nanicão) under specific drying conditions was evaluated regarding its chemical composition and nutritional value. results are expressed in dry weight (dw). the unripe banana flour (ubf) presented a high amount of total dietary fiber (df) (56.24 g/100 g), which consisted of resistant starch (rs) (48.99 g/100 g), fructans (0.05 g/100 g) and df without rs or fructans (7.2 g/100 g). the contents of available starch (as) (27.78 g/100 g) a ...201121732181
the complete chloroplast genome of banana (musa acuminata, zingiberales): insight into plastid monocotyledon evolution.banana (genus musa) is a crop of major economic importance worldwide. it is a monocotyledonous member of the zingiberales, a sister group of the widely studied poales. most cultivated bananas are natural musa inter-(sub-)specific triploid hybrids. a musa acuminata reference nuclear genome sequence was recently produced based on sequencing of genomic dna enriched in nucleus.201323840670
the banana genome hub.banana is one of the world's favorite fruits and one of the most important crops for developing countries. the banana reference genome sequence (musa acuminata) was recently released. given the taxonomic position of musa, the completed genomic sequence has particular comparative value to provide fresh insights about the evolution of the monocotyledons. the study of the banana genome has been enhanced by a number of tools and resources that allows harnessing its sequence. first, we set up essenti ...201323707967
physico-chemical characteristics and sensory evaluation of wheat bread partially substituted with banana (musa acuminata x balbisiana cv. awak) pseudo-stem flour.the physico-chemical and sensorial properties of the control (bctr), commercial wheat flour (cwf) bread substituted with 10% bpf (banana pseudo-stem flour) (b10bpf) and b10bpf with added 0.8% w/w (flour weight basis) xanthan gum (xg) or sodium carboxymethylcellulose (cmc) (b10bpfxg and b10bpfcmc, respectively) were examined. the proximate analyses revealed that the composite bread had significantly higher moisture, ash, crude fibre, soluble, insoluble and total dietary fibre contents but lower p ...201323561142
genetic diversity and population structure of musa accessions in ex situ conservation.banana cultivars are mostly derived from hybridization between wild diploid subspecies of musa acuminata (a genome) and m. balbisiana (b genome), and they exhibit various levels of ploidy and genomic constitution. the embrapa ex situ musa collection contains over 220 accessions, of which only a few have been genetically characterized. knowledge regarding the genetic relationships and diversity between modern cultivars and wild relatives would assist in conservation and breeding strategies. our o ...201323497122
mechanisms of haplotype divergence at the rga08 nucleotide-binding leucine-rich repeat gene locus in wild banana (musa balbisiana).comparative sequence analysis of complex loci such as resistance gene analog clusters allows estimating the degree of sequence conservation and mechanisms of divergence at the intraspecies level. in banana (musa sp.), two diploid wild species musa acuminata (a genome) and musa balbisiana (b genome) contribute to the polyploid genome of many cultivars. the m. balbisiana species is associated with vigour and tolerance to pests and disease and little is known on the genome structure and haplotype d ...201020637079
activity and expression of banana starch phosphorylases during fruit development and ripening.two main forms of starch phosphorylase (ec 2.4.1.1) were identified and purified from banana (musa acuminata colla. cv. nanicão) fruit. one of them, designated phosphorylase i, had a native molecular weight of 155 kda and subunit of 90 kda, a high affinity towards branched glucans and an isoelectric point around 5.0. the other, phosphorylase ii, eluted at a higher salt concentration from the anion exchanger, had a low affinity towards branched glucans, a native molecular weight of 290 kda and su ...200212447547
genome-wide analysis of the ap2/erf family in musa species reveals divergence and neofunctionalisation during evolution.ap2/erf domain containing transcription factor super family is one of the important regulators in the plant kingdom. the involvement of ap2/erf family members has been elucidated in various processes associated with plant growth, development as well as in response to hormones, biotic and abiotic stresses. in this study, we carried out genome-wide analysis to identify members of ap2/erf family in musa acuminata (a genome) and musa balbisiana (b genome) and changes leading to neofunctionalisation ...201626733055
genome-wide identification and characterization of the superoxide dismutase gene family in musa acuminata cv. tianbaojiao (aaa group).superoxide dismutase (sod) is an essential enzyme of the plant antioxidant system that responds to oxidative stresses caused by adverse conditions. banana is an important staple and economic crop in tropical and subtropical regions. however, its growth and yield are constantly affected by various abiotic stresses. to analyze the roles of distinct sod genes under various stresses, a detailed characterization and analysis of the sod gene family in cavendish banana is indispensable.201526486759
a saturated ssr/dart linkage map of musa acuminata addressing genome rearrangements among bananas.the genus musa is a large species complex which includes cultivars at diploid and triploid levels. these sterile and vegetatively propagated cultivars are based on the a genome from musa acuminata, exclusively for sweet bananas such as cavendish, or associated with the b genome (musa balbisiana) in cooking bananas such as plantain varieties. in m. acuminata cultivars, structural heterozygosity is thought to be one of the main causes of sterility, which is essential for obtaining seedless fruits ...201020388207
efficacy of rapd, issr and damd markers in assessment of genetic variability and population structure of wild musa acuminata colla.north east india is considered as one of the major biodiversity hotspots worldwide and centre of origin of several plant species including musa. musa acuminata colla is known to be one of the wild progenitors of cultivated bananas and plantains. three single primer based dna marker techniques viz., random amplified polymorphic dna (rapd), inter simple sequence repeat (issr) and directed amplification of minisatellites dna (damd) were used for diversity diagnostics among 25 genotypes of wild m. a ...201526261399
high resolution mass spectrometry imaging reveals the occurrence of phenylphenalenone-type compounds in red paracytic stomata and red epidermis tissue of musa acuminata ssp. zebrina cv. 'rowe red'.the banana epidermis and in particular their stomata are conducive sites for the penetration of pathogenic fungi which can severely limit global banana production. the red pseudostem of the ornamental banana musa acuminata ssp. zebrina cv. 'rowe red' was used to study the chemical constituents of the epidermal cell layer using matrix-free laser desorption/ionization fourier transform ion cyclotron resonance mass spectrometric imaging (ldi-ft-icr-msi). the high resolution of this technique allowe ...201526004822
transcripts and micrornas responding to salt stress in musa acuminata colla (aaa group) cv. berangan roots.physiological responses to stress are controlled by expression of a large number of genes, many of which are regulated by micrornas. since most banana cultivars are salt-sensitive, improved understanding of genetic regulation of salt induced stress responses in banana can support future crop management and improvement in the face of increasing soil salinity related to irrigation and climate change. in this study we focused on determining mirna and their targets that respond to nacl exposure and ...201525993649
biochemical and in-silico studies on pectin methylesterase from g9 variety of musa acuminata for delayed ripening.ripening of fruit is a very important process but in some fruits early ripening leads to a great damage during long distance transportation. there are various biochemical changes taking place during the phase of ripening of fruit such as changes in respiration, aroma, flavor, ethylene production and activity of cell wall degrading enzymes. some important cell wall degrading enzymes are polygalacturonase (pg), pectin methylesterase (pme), pectin lyase, rgase. pme is known to act as a cell wall hy ...201525926894
effect of banana pulp and peel flour on physicochemical properties and in vitro starch digestibility of yellow alkaline noodles.the present study describes the utilization of banana--cavendish (musa acuminata l., cv cavendshii) and dream (musa acuminata colla. aaa, cv 'berangan')--pulp and peel flours as functional ingredients in yellow alkaline noodles. noodles were prepared by partial substitution of wheat flour with ripe banana pulp or peel flours. in most cases, the starch hydrolysis index, predicted glycaemic index (pgi) and physicochemical properties of cooked noodles were affected by banana flour addition. in gene ...200919757248
antioxidant effcacy of unripe banana (musa acuminata colla) peel extracts in sunflower oil during accelerated storage.sunflower oil is prone to oxidation during storage time, leading to production of toxic compounds that might affect human health. synthetic antioxidants are used to prevent lipid oxidation. spreading interest in the replacement of synthetic food antioxidants by natural ones has fostered research on fruit and vegetables for new antioxidants.201728068040
involvement of a banana mads-box transcription factor gene in ethylene-induced fruit ripening.to investigate the regulation of mads-box genes in banana (musa acuminata l. aaa group cv. brazilian) fruit development and postharvest ripening, we isolated from banana fruit a mads-box gene designated mumads1. amino acid alignment indicated mumads1 belongs to the agamous subfamily, and phylogenetic analysis indicates that this gene is most similar to class d mads-box genes. reverse transcriptase-polymerase chain reaction (rt-pcr) analysis showed that mumads1 is expressed in the stamen and pist ...200918820933
the diversification and activity of hat transposons in musa genomes.sequencing of plant genomes often identified the hat superfamily as the largest group of dna transposons. nevertheless, detailed information on the diversity, abundance and chromosomal localization of plant hat families are rare. by in silico analyses of the reference genome assembly and bacterial artificial chromosome (bac) sequences, respectively, we performed the classification and molecular characterization of hat transposon families in musa acuminata. musa hat transposons are organized in t ...201425377178
enhanced yield of phenolic extracts from banana peels (musa acuminata colla aaa) and cinnamon barks (cinnamomum varum) and their antioxidative potentials in fish oil.the bioactive compounds of banana peels and cinnamon barks were extracted by vacuum microwave and ultrasonic-assisted extraction methods at pre-determined temperatures and times. these methods enhance the yield extracts in shorter time. the highest yields of both extracts were obtained from the conditions which employed the highest temperature and the longest time. the extracts' yield from cinnamon bark method was higher by ultrasonic than vacuum microwave method, while vacuum microwave method g ...201425328205
artificial neural network modelling of the antioxidant activity and phenolic compounds of bananas submitted to different drying treatments.bananas (cv. musa nana and musa cavendishii) fresh and dried by hot air at 50 and 70°c and lyophilisation were analysed for phenolic contents and antioxidant activity. all samples were subject to six extractions (three with methanol followed by three with acetone/water solution). the experimental data served to train a neural network adequate to describe the experimental observations for both output variables studied: total phenols and antioxidant activity. the results show that both bananas are ...201525172734
evasion of short interfering rna-directed antiviral silencing in musa acuminata persistently infected with six distinct banana streak pararetroviruses.vegetatively propagated crop plants often suffer from infections with persistent rna and dna viruses. such viruses appear to evade the plant defenses that normally restrict viral replication and spread. the major antiviral defense mechanism is based on rna silencing generating viral short interfering rnas (sirnas) that can potentially repress viral genes posttranscriptionally through rna cleavage and transcriptionally through dna cytosine methylation. here we examined the rna silencing machinery ...201425056897
cytogenetic evidence of mixed disomic and polysomic inheritance in an allotetraploid (aabb) musa genotype.edible bananas originated mainly from two wild species, musa acuminata colla (aa) and musa balbisiana colla (bb), and triploid cultivars with an aaa, aab or abb genome are the most widely used. in the present study, chromosome pairing affinities are investigated in a sterile ab indian variety and in its fertile colchicine-induced allotetraploid (aabb) derivative to determine the inheritance pattern of the tetraploid genotype. the potential implications of interspecific recombination and chromoso ...201223087127
the natural impact of banana inflorescences (musa acuminata) on human nutrition.banana inflorescences are popularly known as 'navels,' and they are used in brazil as nutritional complements. however, the nutritional value of banana inflorescences (male flowers and bracts) has never been studied. therefore, plant material of musa acuminata, cultivar "ouro", was collected in rio de janeiro state, brazil, and then submitted to chemical procedures to determine its nutritional composition. the experiment was arranged a completely randomized design and performed in triplicate. th ...201223070128
canopy and leaf composition drive patterns of nutrient release from pruning residues in a coffee agroforest.in a coffee agroforest, the crop is cultivated under the shade of fruit-bearing and nitrogen (n)-fixing trees. these trees are periodically pruned to promote flowering and fruiting as well as to make nutrients stored in tree biomass available to plants. we investigated the effect of canopy composition and substrate quality on decomposition rates and patterns of nutrient release from pruning residues in a coffee agroforest located in costa rica's central valley. initial phosphorus (p) release was ...201222827139
structures of chlorophyll catabolites in bananas (musa acuminata) reveal a split path of chlorophyll breakdown in a ripening fruit.the disappearance of chlorophyll is a visual sign of fruit ripening. yet, chlorophyll breakdown in fruit has hardly been explored; its non-green degradation products are largely unknown. here we report the analysis and structure elucidation of colorless tetrapyrrolic chlorophyll breakdown products in commercially available, ripening bananas (musa acuminata, cavendish cultivar). in banana peels, chlorophyll catabolites were found in an unprecedented structural richness: a variety of new fluoresce ...201222807397
new microsatellite markers for bananas (musa spp).thirty-four microsatellite markers (ssrs) were identified in est and bac clones from musa acuminata burmannicoides var. calcutta 4 and validated in 22 musa genotypes from the banana germplasm bank of embrapa-cnpmf, which includes wild and improved diploids. the number of alleles per locus ranged from 2 to 14. the markers were considered highly informative based on their polymorphism information content values; more than 50% were above 0.5. these ssrs will be useful for banana breeding programs, ...201222614278
identification of genes differentially expressed during ripening of banana.the banana (musa acuminata, subgroup cavendish 'grand nain') is a climacteric fruit of economic importance. a better understanding of the banana ripening process is needed to improve fruit quality and to extend shelf life. eighty-four up-regulated unigenes were identified by differential screening of a banana fruit cdna subtraction library at a late ripening stage. the ripening stages in this study were defined according to the peel color index (pci). unigene sequences were analyzed with differe ...200716934912
a ser/thr protein kinase phosphorylates ma-acs1 (musa acuminata 1-aminocyclopropane-1-carboxylic acid synthase 1) during banana fruit ripening.1-aminocyclopropane-1-carboxylic acid synthase (acs) catalyzes the rate-limiting step in ethylene biosynthesis during ripening. acs isozymes are regulated both transcriptionally and post-translationally. however, in banana, an important climacteric fruit, little is known about post-translational regulation of acs. here, we report the post-translational modification of ma-acs1 (musa acuminata acs1), a ripening inducible isozyme in the acs family, which plays a key role in ethylene biosynthesis du ...201222419220
spatial and temporal effects on seed dispersal and seed predation of musa acuminata in southern yunnan, china.wild bananas are abundant in tropical areas and many ecologists have observed that the succession process is quicker following increased disturbance. this study was conducted to analyze animal-seed interactions and their effects on the seed fate of a wild banana species (musa acuminata) in tropical southern yunnan (china) through experiments considering spatial (site and habitat) and temporal (seasons) variation. the largest proportion of fruits (81%) was removed by frugivorous seed dispersers, ...201222405446
supersage combined with pcr walking allows global gene expression profiling of banana (musa acuminata), a non-model organism.super-serial analysis of gene expression (supersage) was used to characterize, for the first time, the global gene expression pattern in banana (musa acuminata). a total of 10,196 tags were generated from leaf tissue, representing 5,292 expressed genes. forty-nine tags of the top 100 most abundantly expressed transcripts were annotated by homology to cdna or est sequences. typically for leaf tissue, analysis of the transcript profiles showed that the majority of the abundant transcripts are invo ...200516133315
isolation and characterization of nucleotide-binding site and c-terminal leucine-rich repeat-resistance gene candidates in bananas.commercial banana varieties are highly susceptible to fungal pathogens, as well as bacterial pathogens, nematodes, viruses, and insect pests. the largest known family of plant resistance genes encodes proteins with nucleotide-binding site (nbs) and c-terminal leucine-rich repeat (lrr) domains. conserved motifs in such genes in diverse plant species offer a means for the isolation of candidate genes in banana that may be involved in plant defense. six degenerate pcr primers were designed to targe ...201122194165
molecular and immunological characterization of mus a 5 allergen from banana fruit.banana fruit has become an important cause of fruit allergy in the recent years. among the five registered iuis allergens, mus a 1 and mus a 2 have been characterized in detail. in this study, molecular characterization and evaluation of the allergenic properties of β-1,3-glucanase from banana (musa acuminata), denoted as mus a 5, were performed.201222162266
repetitive part of the banana (musa acuminata) genome investigated by low-depth 454 sequencing.bananas and plantains (musa spp.) are grown in more than a hundred tropical and subtropical countries and provide staple food for hundreds of millions of people. they are seed-sterile crops propagated clonally and this makes them vulnerable to a rapid spread of devastating diseases and at the same time hampers breeding improved cultivars. although the socio-economic importance of bananas and plantains cannot be overestimated, they remain outside the focus of major research programs. this slows d ...201020846365
an insight into the sequential, structural and phylogenetic properties of banana 1-aminocyclopropane-1-carboxylate synthase 1 and study of its interaction with pyridoxal-5'-phosphate and aminoethoxyvinylglycine.in banana, ethylene production for ripening is accompanied by a dramatic increase in 1-aminocyclopropane-1-carboxylate (acc) content, transcript level of musa acuminata acc synthase 1 (ma-acs1) and the enzymatic activity of acc synthase 1 at the onset of the climacteric period. ma-acs1 catalyses the conversion of s-adenosyl-l-methionine (sam) to acc, the key regulatory step in ethylene biosynthesis. multiple sequence alignments of 1-aminocyclopropane-1-carboxylate synthase (acs) amino acid seque ...201020689184
sap phytochemical compositions of some bananas in thailand.banana sap has some special properties relating to various phenomena such as browning of fruits after harvesting, permanent staining of cloth and fibers, and antioxidant and antibleeding properties. analysis of banana sap using high-performance liquid chromatography-electrospray ionization-mass spectrometry (hplc-esi-ms) indicated the presence of phenolic and aromatic amino compounds of interest due to their special properties. with the online positive electrospray ionization mode (esi), the pos ...201020681667
characterization of novel microsatellite markers in musa acuminata subsp. burmannicoides, var. calcutta 4.banana is a nutritionally important crop across tropical and sub-tropical countries in sub-saharan africa, central and south america and asia. although cultivars have evolved from diploid, triploid and tetraploid wild asian species of musa acuminata (a genome) and musa balbisiana (b genome), many of today's commercial cultivars are sterile triploids or diploids, with fruit developing via parthenocarpy. as a result of restricted genetic variation, improvement has been limited, resulting in a crop ...201020507605
cloning of an adp-ribosylation factor gene from banana (musa acuminata) and its expression patterns in postharvest ripening fruit.a full-length cdna encoding an adp-ribosylation factor (arf) from banana (musa acuminata) fruit was cloned and named maarf. it contains an open reading frame encoding a 181-amino-acid polypeptide. sequence analysis showed that maarf shared high similarity with arf of other plant species. the genomic sequence of maarf was also obtained using polymerase chain reaction (pcr). sequence analysis showed that maarf was a split gene containing five exons and four introns in genomic dna. reverse-transcri ...201020435371
fluorescent chlorophyll catabolites in bananas light up blue halos of cell death.breakdown of chlorophyll is a major contributor to the diagnostic color changes in fall leaves, and in ripening apples and pears, where it commonly provides colorless, nonfluorescent tetrapyrroles. in contrast, in ripening bananas (musa acuminata) chlorophylls fade to give unique fluorescent catabolites (fccs), causing yellow bananas to glow blue, when observed under uv light. here, we demonstrate the capacity of the blue fluorescent chlorophyll catabolites to signal symptoms of programmed cell ...200919805212
accumulation of soluble sugars in peel at high temperature leads to stay-green ripe banana fruit.bananas (musa acuminata, aaa group) fail to develop a yellow peel and stay green when ripening at temperatures >24 degrees c. the identification of the mechanisms leading to the development of stay-green ripe bananas has practical value and is helpful in revealing pathways involved in the regulation of chlorophyll (chl) degradation. in the present study, the chl degradation pathway was characterized and the progress of ripening and senescence was assessed in banana peel at 30 degrees c versus 20 ...200919700495
development and assessment of diversity arrays technology for high-throughput dna analyses in musa.diversity arrays technology (dart) is a dna hybridisation-based molecular marker technique that can detect simultaneously variation at numerous genomic loci without sequence information. this efficiency makes it a potential tool for a quick and powerful assessment of the structure of germplasm collections. this article demonstrates the usefulness of dart markers for genetic diversity analyses of musa spp. genotypes. we developed four complexity reduction methods to generate dart genomic represen ...200919693484
chemical composition and physicochemical properties of green banana (musa acuminata x balbisiana colla cv. awak) flour.flour was prepared from peeled and unpeeled banana awak abb. samples prepared were subjected to analysis for determination of chemical composition, mineral, dietary fibre, starch and total phenolics content, antioxidant activity and pasting properties. in general, flour prepared from unpeeled banana was found to show enhanced nutrition values with higher contents of mineral, dietary fibre and total phenolics. hence, flour fortified with peel showed relatively higher antioxidant activity. on the ...200919449278
differential transcriptional regulation of banana sucrose phosphate synthase gene in response to ethylene, auxin, wounding, low temperature and different photoperiods during fruit ripening and functional analysis of banana sps gene promoter.sucrose phosphate synthase (sps) (ec 2.3.1.14) is the key regulatory component in sucrose formation in banana (musa acuminata subgroup cavendish, cv giant governor) fruit during ripening. this report illustrates differential transcriptional responses of banana sps gene following ethylene, auxin, wounding, low temperature and different photoperiods during ripening in banana fruit. whereas ethylene strongly stimulated sps transcript accumulation, auxin and cold treatment only marginally increased ...200818830708
[development and application of est-derived ssr markers for bananas (musa nana lour.)].a total of 2,284 banana unigene sequences were mined, resulting in the identification of 122 ssrs in 110 sequences. among them, there were 37 motifs the overall average length of ssrs was 20 bp. the trinucleotide repeats appeared to be the most abundant ssrs (47.6%), followed by dinucleotide repeats (33.1%), whereas the tetranucleotide repeats were less abundant. the rich repeats ag and agg were predominant, accounting for 75.7% and 36% in dri- and tri-nucleotide repeats respectively. a total of ...200818779139
identification of cytoplasmic ancestor gene-pools of musa acuminata colla and musa balbisiana colla and their hybrids by chloroplast and mitochondrial haplotyping.cytoplasmically inherited characters such as resistance to viral and fungal diseases, determination of starch types, crop yield, resistance to low or high temperature often contribute to the advantageous phenotypic traits of plants. in the present study, our goal was to elucidate the genealogy of cytoplasmic genomes chloroplast and mitochondria in banana. banana breeding is rather complicated because of the low fertility and mostly unknown origin of the edible cultivars, therefore, knowledge on ...200818762903
isomeric oxabenzochrysenones from musa acuminata and wachendorfia thyrsiflora.two new oxabenzochrysenones (naphthoxanthenones), representing phenylphenalenone-related natural products, were isolated from musa acuminata and wachendorfia thyrsiflora.200212434989
c-terminal phosphorylation is essential for regulation of ethylene synthesizing acc synthase enzyme.the genetic and molecular biological studies mainly in arabidopsis and in some other plants have begun to uncover the various components of ripening signaling pathway in plants. although transcriptional regulation of major ripening genes have been studied in detail, information on role of phosphorylation in regulating the activity and stability of core ripening pathway associated proteins in relation to ethylene biosynthesis during fruit ripening is still limited. recently we have demonstrated t ...201323221778
characterization of transcriptional profiles of ma-acs1 and ma-aco1 genes in response to ethylene, auxin, wounding, cold and different photoperiods during ripening in banana fruit.the ripening-specific genes ma-acs1 (musa acuminata acc synthase1) and ma-aco1 (m. acuminata acc oxidase 1) are regulated in response to a wide variety of factors. here, we have studied the differential transcript accumulation pattern and protein levels of ma-acs1 and ma-aco1 genes in response to ethylene, auxin, wounding and low temperature in preclimacteric banana fruit. we have shown that exogenous application of ethylene and auxin induced the expression of ma-acs1, while ma-aco1 showed margi ...200818554749
genomes, diversity and resistance gene analogues in musa species.resistance genes (r genes) in plants are abundant and may represent more than 1% of all the genes. their diversity is critical to the recognition and response to attack from diverse pathogens. like many other crops, banana and plantain face attacks from potentially devastating fungal and bacterial diseases, increased by a combination of worldwide spread of pathogens, exploitation of a small number of varieties, new pathogen mutations, and the lack of effective, benign and cheap chemical control. ...200818544928
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