Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| structure characteristics of an acidic polysaccharide purified from banana (musa nana lour.) pulp and its enzymatic degradation. | banana is one of the most important fruits over the world. the chemical composition is critical for the organoleptic properties and health benefits. as one of the leading bioactive components in banana pulp, the polysaccharides may contribute to the beneficial health effects. however, their precise structure information remains unknown. a leading acidic polysaccharide (abpp) of banana pulp was purified and identified by nuclear magnetic resonnance spectroscopy (nmr) and gas chromatography-mass s ... | 0 | 28336277 |
| genome-wide analyses of sweet family proteins reveal involvement in fruit development and abiotic/biotic stress responses in banana. | sugars will eventually be exported transporters (sweet) are a novel type of sugar transporter that plays crucial roles in multiple biological processes. from banana, for the first time, 25 sweet genes which could be classified into four subfamilies were identified. majority of masweets in each subfamily shared similar gene structures and conserved motifs. comprehensive transcriptomic analysis of two banana genotypes revealed differential expression patterns of masweets in different tissues, at v ... | 0 | 28615718 |
| lineage-specific evolutionary histories and regulation of major starch metabolism genes during banana ripening. | starch is the most widespread and abundant storage carbohydrate in plants. it is also a major feature of cultivated bananas as it accumulates to large amounts during banana fruit development before almost complete conversion to soluble sugars during ripening. little is known about the structure of major gene families involved in banana starch metabolism and their evolution compared to other species. to identify genes involved in banana starch metabolism and investigate their evolutionary history ... | 0 | 27994606 |
| traditional uses, phytochemistry and pharmacology of wild banana (musa acuminata colla): a review. | musa acuminata, the wild species of banana is a plant of the tropical and subtropical regions. over the past few decades, the health benefits of m. acuminata have received much attention. all parts of the plant including fruits, peel, pseudostem, corm, flowers, leaves, sap and roots have found their use in the treatment of many diseases in traditional medicine. literature review have indicated use of m. acuminata in the treatment of various diseases such as fever, cough, bronchitis, dysentery, a ... | 0 | 27988402 |
| the banana (musa acuminata) genome and the evolution of monocotyledonous plants. | bananas (musa spp.), including dessert and cooking types, are giant perennial monocotyledonous herbs of the order zingiberales, a sister group to the well-studied poales, which include cereals. bananas are vital for food security in many tropical and subtropical countries and the most popular fruit in industrialized countries. the musa domestication process started some 7,000 years ago in southeast asia. it involved hybridizations between diverse species and subspecies, fostered by human migrati ... | 0 | 22801500 |
| stomatal density and responsiveness of banana fruit stomates. | determination of stomatal densities of the banana peel (musa acuminata l. var hort. valery) by microscopic observations showed 30 times fewer stomates on fruit epidermis than found on the banana leaf. observations also showed that peel stomates were not laid down in a linear pattern as on the leaf.it was demonstrated that stomatal responses occurred in banana fruit. specific conditions of high humidity and light were necessary for stomatal opening: low humidity and darkness were necessary for cl ... | 1966 | 16656239 |
| reinitiation of vegetative growth from aseptically cultured terminal floral apex of banana. | terminal floral apices of musa acuminata cv. dwarf cavendish were isolated and cultured in a modified murashige and skoog (1962) medium supplemented with n6-benzylamino purine (5 mg/l) and 10% (v/v) coconut water. under these conditions, the determinate floral buds are transformed into a multiplying vegetative shoot system. rooted plantlets were obtained by treating shoots with the auxin, naphthaleneacetic acid (1 mg/l), and activated charcoal (0.025%). practical benefits resulting from these ob ... | 1985 | 11538837 |
| banana ripening: implications of changes in internal ethylene and co(2) concentrations, pulp fructose 2,6-bisphosphate concentration, and activity of some glycolytic enzymes. | in ripening banana (musa acuminata l. [aaa group, cavandish subgroup] cv. valery) fruit, the steady state concentration of the glycolytic regulator fructose 2,6-bisphosphate (fru 2,6-p(2)) underwent a transient increase 2 to 3 hours before the respiratory rise, but coincident with the increase in ethylene synthesis. fru 2,6-p(2) concentration subsequently decreased, but increased again approximately one day after initiation of the respiratory climacteric. this second rise in fru 2,6-p(2) continu ... | 1987 | 16665670 |
| wedelolactone and coumestan derivatives as new antihepatotoxic and antiphlogistic principles. | for the study of structure-activity relationships, the antihepatotoxic wedelolactone (7-methoxy-5,11,12-trihydroxy-coumestan) and 6 coumestan derivatives were synthesized by the application of a modified method of wanzlich. an evaluation of the biological characteristics of the synthetic compounds and acuminatin from musa acuminata showed that most of the wedelolactone derivatives significantly protected primary cultured liver cells from the toxicity of ccl4, galactosamine (galc), and phalloidin ... | 1988 | 2458108 |
| somatic embryogenesis and plants from immature zygotic embryos of the species musa acuminata and musa balbisiana. | callus was obtained from immature zygotic embryos of semminiferous species (diploids) of musa sp. using a medium derived from that of murashige and skoog. picloram (7.5 μm) was added and the medium was solidified with gelrite (2 gl(-1)). differentiation of the first somatic embryos occurred after transfer of the callus in the presence of 7.5 μm picloram or 5.3 μm naa. somatic embryos germinated on the medium supplemented with 5.3 μm naa. serial sections of zygotic and somatic embryos showed perf ... | 1989 | 24240457 |
| monoxenic culture of banana-parasitic nematodes on musa acuminata cv. poyo shoots. | 1990 | 19287766 | |
| differential protein accumulation in banana fruit during ripening. | banana (musa acuminata, cv dwarf cavendish) proteins were extracted from pulp tissue at different stages of ripening and analyzed by two-dimensional electrophoresis. the results provide evidence of differential protein accumulation during ripening. two sets of polypeptides have been detected that increase substantially in ripe fruit. these polypeptides were characterized as glycoproteins by western blotting and concanavalin a binding assays. antibodies againts tomato polygalacturonase cross-reac ... | 1992 | 16668607 |
| a molecular marker-based linkage map of diploid bananas (musa acuminata). | a partial molecular linkage map of the musa acuminata diploid genome is presented. this map is based on 58 rflp, four isozyme and 28 rapd markers segregating in an f2 population of 92 individuals. a total of 90 loci was detected, 77 of which were placed on 15 linkage groups while 13 segregated independently. segregation distortions were shown by 36% of all loci, mostly favoring the male parent. chromosome structural rearrangements were believed to be one of the main causes of these distortions. ... | 1993 | 24190325 |
| maternal inheritance of chloroplast genome and paternal inheritance of mitochondrial genome in bananas (musa acuminata). | restriction fragment length polymorphisms (rflps) were used as markers to determine the transmission of cytoplasmic dna in diploid banana crosses. progenies from two controlled crosses were studied with heterologous cytoplasmic probes. this analysis provided evidence for a strong bias towards maternal transmission of chloroplast dna and paternal transmission of mitochondrial dna in musa acuminata. these results suggest the existence of two separate mechanisms of organelle transmission and select ... | 1994 | 7923414 |
| dna profiling of banana and plantain cultivars using random amplified polymorphic dna (rapd) and restriction fragment length polymorphism (rflp) markers. | polymerase chain reaction (pcr) amplification of genomic dna from 57 musa cultivars with 60 random 10-mer primers generated 605 polymorphic amplification products which were useful in unambiguous cultivar identifications. unweighted pair-group method analysis of this data grouped the cultivars into specific clusters depending on their genomic similarities. the diploid ancestral species of cultivated banana and plantains, namely musa acuminata sp malaccensis, an a genome donor and m. balbisiana, ... | 1995 | 8582364 |
| use of competitive pcr to assay copy number of repetitive elements in banana. | banana is one of the most important subtropical fruit crops. genetic improvement by traditional breeding strategies is difficult and better knowledge of genomic structure is needed. repeated sequences are powerful markers for genetic fingerprinting. the method proposed here to determine the copy number of nuclear repetitive elements is based on competitive reverse transcription-polymerase chain reaction and can also be used for quantifying cytosolic sequences. the reliability of this method was ... | 1996 | 9003287 |
| differential gene expression in ripening banana fruit. | during banana (musa acuminata l.) fruit ripening ethylene production triggers a developmental cascade that is accompanied by a massive conversion of starch to sugars, an associated burst of respiratory activity, and an increase in protein synthesis. differential screening of cdna libraries representing banana pulp at ripening stages 1 and 3 has led to the isolation of 11 nonredundant groups of differentially expressed mrnas. identification of these transcripts by partial sequence analysis indica ... | 1997 | 9342866 |
| colonization pattern of aquatic hyphomycetes on leaf packs in subtropical stream. | steralized leaf pieces of eight plants (eucalyptus rostrata, phoenix dactylifera, phragmites australis, musa nana, salix subserrata, cyperus alopecuroides, ricinus communis and eichhornia crassipes) were submerged in the nile stream. thirty-nine species of aquatic hyphomycetes were colonized on the plant leaves. eucalyptus was the best substratum (30 species) for colonization by aquatic hyphomycetes. phoenix (14 species), phragmites (11 species), salix (9 species), musa (8 species), ricinus and ... | 1997 | 16283114 |
| banana sucrose-phosphate synthase gene expression during fruit ripening. | a 952-base pair polymerase-chain-reaction product of sucrose-phosphate synthase (sps) (ec 2.3.1.14) from banana (musa acuminata cv. nanicão) fruit was cloned and used to study expression of the enzyme during development and ripening. the deduced amino acid sequence shows that banana sps has a high homology with the leaf, tap-root and bean enzymes from other species. enzyme activity, and mrna and protein levels point to an increase in sps expression during ripening. the accumulation of sucrose wa ... | 1997 | 9431676 |
| curative and residual efficacy of injection applications of avermectins for control of plant-parasitic nematodes on banana. | studies were conducted to determine the curative and residual efficacy of avermectins at controlling plant-parasitic nematodes when injected into the pseudostem of banana, musa acuminata cv. cavendish. in addition, we determined the lowest concentration of avermectins that provided satisfactory efficacy as protectants when injected into banana pseudostems. experiments were conducted with a root-knot nematode, meleidogyne javanica, and the burrowing nematode, radopholus similis. injections (1 ml) ... | 1997 | 19274271 |
| phenylphenalenone-type phytoalexins from unripe buñgulan banana fruit. | fourteen phenylphenalenone-type phytoalexins (1-14), including three new compounds, were isolated from the peel of unripe musa acuminata [aaa] cv. buñgulan fruit which had been injured and then inoculated with conidia of colletotrichum musae. these new phytoalexins were identified as (+)-cis-2,3-dihydro-2,3-dihydroxy-4-(4'-hydroxyphenyl)phenalen-1-one (12), 9-(3',4'-dimethoxyphenyl)-2-methoxyphenalen-1-one (13) and 9-(4'-hydroxyphenyl)-2-methoxyphenalen-1-one (14). the ratios of the relative int ... | 1998 | 27393357 |
| the abundant 31-kilodalton banana pulp protein is homologous to class-iii acidic chitinases. | we have identified and characterized the abundant protein from the pulp of banana fruit (musa acuminata cv. grand nain), and have isolated a cdna clone encoding this protein. comparison of the amino terminal sequence of the purified 31 kda protein (p31) suggests that it is related to plant chitinases. western analyses utilizing rabbit anti-p31 antiserum demonstrate that this protein is pulp-specific in banana. a full-length cdna clone homologous to class iii acidic chitinase genes has been isola ... | 1998 | 9461677 |
| diploid musa acuminata genetic diversity assayed with sequence-tagged microsatellite sites. | the sequence-tagged microsatellite site (stms) discrimination potential was explored using nine microsatellite primer pairs. stms polymorphism was assayed by nonradioactive urea-polyacrylamide gel electrophoresis. genetic relationships were examined among 59 genotypes of wild or cultivated accessions of diploid musa acuminata. the organization of the subspecies was confirmed and some clone relationships were clarified. | 1998 | 9694284 |
| multiple forms of polygalacturonase from banana fruits. | three multiple forms of polygalacturonase (pg) in ripe and two in unripe banana (musa acuminata) fruits were separated by deae-cellulose and further purified using sephadex g-150 chromatography. the multiple forms can be differentiated from each other on the basis of their properties. pg1 and pg3 were identified as endo-pg and pg2 as exo-pg on the basis of decrease in viscosity, increase in reducing sugar and the reaction product. pg2 and pg3 increased with the ripening of fruits. pg1, pg2 and p ... | 1998 | 9637063 |
| the selective increase or decrease of organellar dna in generative cells just after pollen mitosis one controls cytoplasmic inheritance. | organellar dna in mature pollen grains of eight angiosperm species (actinidia deliciosa lindl., antirrhinum majus l., arabidopsis thaliana (l.) heynh., medicago sativa l., musa acuminata colla, pelargonium zonale (l.) l'hér, petunia hybrida vilm. and rhododendron mucronatum (blume) g. don, in which the modes of organellar inheritance have been determined genetically, was observed by fluorescence microscopy using technovit 7100 resin sections double-stained with 4',6-diamidino-2-phenylindole (dap ... | 1999 | 10467031 |
| synthesis of 2-(4-hydroxyphenyl)naphthalene-1,8-dicarboxylic anhydride, a phytoalexin isolated from unripe banana (musa acuminata). | 2-(4-hydroxyphenyl)naphthalene-1,8-dicarboxylic anhydride, a component of the phytoalexin that has been isolated from the peel of unripe banana (musa acuminata), was synthesized from 3-bromoacenaphthene. | 1999 | 26300176 |
| changes in the content and biosynthesis of phytoalexins in banana fruit. | changes in the phytoalexin content in unripe fruit of banana, musa acuminata, were analyzed after various treatments. the results show that level of hydroxyanigorufone started to increase 1-2 day after either wounding or inoculation with conidia of colletotrichum musae. inoculation followed by wounding induced the formation of many other phenylphenalenones. the accumulation of hydroxyanigorufone decreased, after its transient maximum, on ripening by exposure of the wounded fruit to ethylene. the ... | 2000 | 11129580 |
| isolation of (s)-(+)-naproxene from musa acuminata. inhibitory effect of naproxene and its 7-methoxy isomer on constitutive cox-1 and inducible cox-2. | the isolation and characterisation of (s)-(+)-6-methoxy-alpha-methyl-2-naphthaleneacetic acid, a well known synthetic non-steroidal anti-inflammatory drug (naproxene), from a natural source is described for the first time. we evaluated the ability of naproxene and its 7-methoxy isomer to abrogate constitutive cox-1 and inducible cox-2 activity in human a549 cells. naproxene inhibited cox-1 (ic50 = 3.42 microm) and cox-2 (ic50 = 1.53 microm), whereas the 7-methoxy isomer had no appreciable effect ... | 2000 | 10909271 |
| genetic repair of mutations in plant cell-free extracts directed by specific chimeric oligonucleotides. | chimeric oligonucleotides are synthetic molecules comprised of rna and dna bases assembled in a double hairpin conformation. these molecules have been shown to direct gene conversion events in mammalian cells and animals through a process involving at least one protein from the dna mismatch repair pathway. the mechanism of action for gene repair in mammalian cells has been partially elucidated through the use of a cell-free extract system. recent experiments have expanded the utility of chimeric ... | 2000 | 10859173 |
| fruit-specific lectins from banana and plantain. | one of the predominant proteins in the pulp of ripe bananas (musa acuminata l.) and plantains (musa spp.) has been identified as a lectin. the banana and plantain agglutinins (called banlec and planlec, respectively) were purified in reasonable quantities using a novel isolation procedure, which prevented adsorption of the lectins onto insoluble endogenous polysaccharides. both banlec and planlec are dimeric proteins composed of two identical subunits of 15 kda. they readily agglutinate rabbit e ... | 2000 | 11030554 |
| identification and chromosomal localization of the monkey retrotransposon in musa sp. | retroelements are ubiquitous features of eukaryotic genomes, often accounting for a substantial fraction of their total dna content. one major group of retroelements, which includes the gypsy and copia-like elements, is distinguished by the presence of long terminal repeats (ltrs). we have identified and partially characterized a sequence from banana (musa acuminata cv. grand nain) which shows significant homology to gypsy-like ltr retroelements from other species. the element, named monkey, sho ... | 2000 | 10954075 |
| purification and structural analysis of an abundant thaumatin-like protein from ripe banana fruit. | the pulp of ripe bananas (musa acuminata) contains an abundant thaumatin-like protein (tlp). characterization of the protein and molecular cloning of the corresponding gene from banana demonstrated that the native protein consists of a single polypeptide chain of 200 amino acid residues. molecular modelling further revealed that the banana thaumatin-like protein (ban-tlp) adopts an overall fold similar to that of thaumatin and thaumatin-like pr-5 proteins. although the banana protein exhibits an ... | 2000 | 11144263 |
| delayed ripening of banana fruit by salicylic acid. | salicylic acid treatment has been found to delay the ripening of banana fruits (musa acuminata). fruit softening, pulp:peel ratio, reducing sugar content, invertase and respiration rate have been found to decrease in salicylic acid treated fruits as compared with control ones. the activities of major cell wall degrading enzymes, viz. cellulase, polygalacturonase and xylanase were found to be decreased in presence of salicylic acid. the major enzymatic antioxidants namely, catalase and peroxidase ... | 2000 | 10996248 |
| combined effect of high pressure, temperature and holding time on polyphenoloxidase and peroxidase activity in banana (musa acuminata). | polyphenoloxidase and peroxidase enzyme activities were evaluated following combined pressure, temperature and holding time treatment in banana (musa acuminata). using pressures of up to 110 mpa, temperatures of up to 70 °c and holding times of up to 25 min, based on a 23 central composite design, the interactive effects were found to significantly influence the activity of both enzymes in prepared banana pulp. temperature and pressure were found to influence the inactivation of polyphenoloxidas ... | 2000 | 29345795 |
| inhibition of beta-amylase activity, starch degradation and sucrose formation by indole-3-acetic acid during banana ripening. | in order to observe the effect of indole-3-acetic acid (iaa) on carbohydrate metabolism, unripe banana (musa acuminata aaa, cv. nanicão) slices were infiltrated with the hormone and left to ripen under controlled conditions. the climacteric respiration burst was reduced by the action of iaa, and starch degradation and sucrose formation were delayed. sucrose synthase (susy; ec 2.4.1.13) and sucrose-phosphate synthase (sps; ec 2.4.1.14) activities and transcript levels were not affected, indicatin ... | 2001 | 11346957 |
| carbohydrate binding properties of banana (musa acuminata) lectin ii. binding of laminaribiose oligosaccharides and beta-glucans containing beta1,6-glucosyl end groups. | this paper extends our knowledge of the rather bizarre carbohydrate binding poperties of the banana lectin (musa acuminata). although a glucose/mannose binding protein which recognizes alpha-linked gluco-and manno-pyranosyl groups of polysaccharide chain ends, the banana lectin was shown to bind to internal 3-o-alpha-d-glucopyranosyl units. now we report that this lectin also binds to the reducing glucosyl groups of beta-1,3-linked glucosyl oligosaccharides (e.g. laminaribiose oligomers). additi ... | 2001 | 11322881 |
| analysis of the distribution and structure of integrated banana streak virus dna in a range of musa cultivars. | summary banana streak virus strain ol (bsv-ol) commonly infects new musa hybrids, and this infection is thought to arise de novo from integrated virus sequences present in the nuclear genome of the plant. integrated dna (musa6+8 sequence) containing the whole genome of the virus has previously been cloned from cv. obino l'ewai (musa aab group), a parent of many of the hybrids. using a southern blot hybridization assay, we have examined the distribution and structure of integrated bsv-ol sequence ... | 2001 | 20573008 |
| carbohydrate binding properties of banana (musa acuminata) lectin i. novel recognition of internal alpha1,3-linked glucosyl residues. | examination of lectins of banana (musa acuminata) and the closely related plantain (musa spp.) by the techniques of quantitative precipitation, hapten inhibition of precipitation, and isothermal titration calorimetry showed that they are mannose/glucose binding proteins with a preference for the alpha-anomeric form of these sugars. both generate precipitin curves with branched chain alpha-mannans (yeast mannans) and alpha-glucans (glycogens, dextrans, and starches), but not with linear alpha-glu ... | 2001 | 11322880 |
| nuclear dna content and base composition in 28 taxa of musa. | the nuclear dna content of 28 taxa of musa was assessed by flow cytometry, using line pxpc6 of petunia hybrida as an internal standard. the 2c dna value of musa balbisiana (bb genome) was 1.16 pg, whereas musa acuminata (aa genome) had an average 2c dna value of 1.27 pg, with a difference of 11% between its subspecies. the two haploid (ic) genomes, a and b, comprising most of the edible bananas, are therefore of similar size, 0.63 pg (610 million bp) and 0.58 pg (560 million bp), respectively. t ... | 2001 | 11550896 |
| activity and expression of banana starch phosphorylases during fruit development and ripening. | two main forms of starch phosphorylase (ec 2.4.1.1) were identified and purified from banana (musa acuminata colla. cv. nanicão) fruit. one of them, designated phosphorylase i, had a native molecular weight of 155 kda and subunit of 90 kda, a high affinity towards branched glucans and an isoelectric point around 5.0. the other, phosphorylase ii, eluted at a higher salt concentration from the anion exchanger, had a low affinity towards branched glucans, a native molecular weight of 290 kda and su ... | 2002 | 12447547 |
| the cloning of ty1-copia-like retrotransposons from 10 varieties of banana (musa sp.). | ty1-copia-like retrotransposons have been identified and investigated in several plant species. here, the internal region of the reverse transcriptase (rt) gene of ty1-copia-like retrotransposons was amplified by pcr from total genomic dna of 10 varieties of banana. two to four clones from each variety were sequenced. extreme heterogeneity in the sequences of ty1-copia-like retrotransposons from all the varieties was revealed following sequence analysis of the reverse transcriptase (rt) fragment ... | 2002 | 12186754 |
| ascertaining maternal and paternal lineage within musa by chloroplast and mitochondrial dna rflp analyses. | in banana, the maternal transmission of chloroplast dna and paternal transmission of the mitochondrial dna provides an exceptional opportunity for studying the maternal and paternal lineage of clones. in the present study, rflp combined with hybridization of heterologous mitochondrial and chloroplastic probes have been used to characterize 71 wild accessions and 131 diploid and 103 triploid cultivated clones. in additon to musa acuminata and musa balbisiana, other species from the four musa sect ... | 2002 | 12175071 |
| isolation, characterization and chromosome localization of repetitive dna sequences in bananas (musa spp.). | partial genomic dna libraries were constructed in musa acuminata and m. balbisiana and screened for clones carrying repeated sequences, and sequences carrying rdna. isolated clones were characterized in terms of copy number, genomic distribution in m. acuminata and m. balbisiana, and sequence similarity to known dna sequences. ribosomal rna genes have been the most abundant sequences recovered. fish with probes for dna clones radkal and radka7, which carry different fragments of musa 26s rdna, a ... | 2002 | 11993938 |
| dimeric phenylphenalenones from musa acuminata and various haemodoraceae species. crystal structure of anigorootin. | three fused octacyclic phenylphenalenone dimers were isolated from musa acuminata: anigorootin, which was first isolated from anigozanthos flavidus and hitherto represented the only compound of that type, the new 4'-hydroxy-anigorootin, and 4',4"-di-hydroxy-anigorootin, which is a revised structure. the crystal structure of anigorootin was determined by x-ray crystallography. 3,3'-bis-hydroxyanigorufone, a dimer of the conventional type known from anigozanthos preissii, was also found in musa ac ... | 2002 | 11985853 |
| differential expression and characterization of three metallothionein-like genes in cavendish banana (musa acuminata). | metallothioneins (mts) are cysteine-rich polypeptides that are involved in metal detoxification and homeostasis in both prokaryotes and eukaryotes. in this study, we report the isolation and characterization of three members (mt2a, mt2b and mt3) of the mt-like gene family from ripening banana fruit and their differential expression in various banana organs and during fruit development and ripening. all members of the mt-like gene encode small cysteine-rich polypeptides of 65-79 amino acid residu ... | 2002 | 11903971 |
| genetic diversity in musa acuminata colla and musa balbisiana colla and some of their natural hybrids using aflp markers. | genetic diversity and relationships were assessed in 28 accessions of musa acuminata (aa) colla and musa balbisiana (bb) colla, and some of their natural hybrids, using the amplified fragment length polymorphisms (aflp) technique. fifteen aflp +3 primer pairs produced 527 polymorphic bands among the accessions. neighbor-joining and principal co-ordinate (pco) analyses using jaccard's similarity coefficient produced four major clusters that closely corresponded with the genome composition of the ... | 2002 | 12582577 |
| analysis of genetic diversity and sectional relationships in musa using aflp markers. | the aflp technique was used to assess the genetic diversity and sectional relationships in 39 accessions representing the four main sections of the genus musa. eight aflp + 3 primer pairs produced 260 polymorphic bands that were used in cluster and pco analysis. a wide range of variability was observed among the species within the sections of the genus musa. aflp data was useful in separating the different sections of the genus as well as differentiating the different genomic groups of section e ... | 2002 | 12582576 |
| isomeric oxabenzochrysenones from musa acuminata and wachendorfia thyrsiflora. | two new oxabenzochrysenones (naphthoxanthenones), representing phenylphenalenone-related natural products, were isolated from musa acuminata and wachendorfia thyrsiflora. | 2002 | 12434989 |
| characterization of resistant starch type iii from banana (musa acuminata). | banana starch (musa acuminata var. nandigobe) was evaluated for its use in generating resistant starch (rs) type iii. structural, physicochemical, and biological properties of these products were analyzed. the investigated process includes debranching of the native starch and retrogradation under different storage temperatures and starch concentrations. after enzymatic debranching, a high amount of low-molecular-weight polymers with a degree of polymerization between 10 and 35 glucose units besi ... | 2002 | 12188636 |
| malate synthase gene expression during fruit ripening of cavendish banana (musa acuminata cv. williams). | malate synthase (ms) is a key enzyme responsible for malic acid synthesis in the glyoxylate cycle, which functions to convert stored lipids to carbohydrates, by catalysing the glyoxylate condensation reaction with acetyl-coa in the peroxisome. in this study, the cloning of an ms cdna, designated mams-1, from the banana fruit is reported. mams-1 was 1801 bp in length encoding a single polypeptide of 556 amino acid residues. sequence analysis revealed that mams-1 possessed the conserved catalytic ... | 2003 | 12493858 |
| pcr-rflp of the ribosomal dna internal transcribed spacers (its) provides markers for the a and b genomes in musa l. | musa acuminata colla (aa genomes) and musa balbisiana colla (bb genomes) are the diploid ancestors of modern bananas that are mostly diploid or triploid cultivars with various combinations of the a and b genomes, including aa, aaa, bb, aab and abb. the objective of this study was to identify molecular markers that will facilitate discrimination of the a and b genomes, based on restriction-site variations in the internal transcribed spacers (its) of the nuclear ribosomal rna genes. the its region ... | 2003 | 12955208 |
| construction and characterization of a bacterial artificial chromosome library of banana (musa acuminata colla). | a bacterial artificial chromosome (bac) library for banana was constructed from leaves of the wild diploid 'calcutta 4' clone (musa acuminata subsp. burmannicoides 2n = 2 x = 22). 'calcutta 4' is widely used in breeding programs for its resistance to the current major disease of banana and is being used to build a genetic reference map of banana. as banana leaves are particularly rich in polyphenols and polysaccharides a protocol was adapted to isolate intact nuclei and high-molecular-weight (hm ... | 2003 | 12671759 |
| expression of a ripening-related cytochrome p450 cdna in cavendish banana (musa acuminata cv. williams). | as part of a study to understand the molecular basis of fruit ripening, this study reports the isolation and characterization of a banana cytochrome p450 (p450) cdna, designated as map450-1, which was associated with fruit ripening of banana. map450-1 encoded a single polypeptide of 507 amino acid residues that shared an overall identity of 27-45% with that of several plant p450s, among which map450-1 was most related phylogenetically to the avocado p450 cyp71a1. the polypeptide that possessed r ... | 2003 | 12594049 |
| sectional relationships in the genus musa l. inferred from the pcr-rflp of organelle dna sequences. | the objective of this study was to construct a molecular phylogeny of the genus musa using restriction-site polymorphisms of the chloroplast (cpdna) and mitochondrial dna (mtdna). six cpdna and two mtdna sequences were amplified individually in polymerase chain reaction (pcr) experiments in 13 species representing the four sections of musa. ensete ventricosum (w.) ch. was used as the outgroup. the amplified products were digested with ten restriction endonucleases. a total of 79 restriction-site ... | 2003 | 12827254 |
| effect of menadione sodium bisulfite, an inducer of plant defenses, on the dynamic of banana phytoalexin accumulation during pathogenesis. | using an authentic sample of 2-hydroxy-9-(p-hydroxyphenyl)-phenalen-1-one, a banana phenalenone-type phytoalexin, we studied its dynamic of accumulation during pathogenesis of banana plants (musa acuminata (aaa), grand nain) inoculated with fusarium oxysporum f.sp. cubense (foc), race 4, the causal agent of panama disease. the results obtained demonstrate that banana plants treated prior inoculation with menadione sodium bisulfite (msb), an inducer of plant defenses, are capable of changing the ... | 2003 | 12926878 |
| soluble and wall-bound phenolics and phenolic polymers in musa acuminata roots exposed to elicitors from fusarium oxysporum f.sp. cubense. | the accumulation of soluble and wall-bound phenolics and phenolic polymers in musa acuminata roots exposed to cell wall-derived elicitor from the pathogen, fusarium oxysporum, f.sp. cubense, race four, was investigated. the root tissue from the banana cultivar "goldfinger" was found to respond strongly and rapidly towards the elicitor through the increased synthesis of phenolic compounds. following elicitation, the conjugated and non-conjugated phenolic metabolites in the induced root tissue wer ... | 2003 | 12842140 |
| identification of genetic markers linked to banana streak disease expression in inter-specific musa hybrids. | recently-introduced inter-specific musa hybrids, bred for improved yield and resistance to diseases, have been found to be widely infected with banana streak virus (bsv), the causal agent of banana streak disease (bsd). one hypothesis suggests: (1) that bsd occurrence in these inter-specific hybrids results from activation of bsv-ol endogenous pararetrovirus sequences (eprv) integrated into the musa genome rather than from external sources of infection, and (2) that the process of genetic hybrid ... | 2003 | 12595986 |
| creation of a bac resource to study the structure and evolution of the banana (musa balbisiana) genome. | the first bacterial artificial chromosome (bac) library of the banana species musa balbisiana 'pisang klutuk wulung' (pkw bac library) was constructed and characterized. one improved and one novel protocol for nuclei isolation were employed to overcome problems caused by high levels of polyphenols and polysaccharides present in leaf tissues. the use of flow cytometry to purify cell nuclei eliminated contamination with secondary metabolites and plastid dna. furthermore, the usefulness of the indu ... | 2004 | 15644977 |
| light- and singlet oxygen-mediated antifungal activity of phenylphenalenone phytoalexins. | the light-induced singlet oxygen production and antifungal activity of phenylphenalenone phytoalexins isolated from infected banana plants (musa acuminata) are reported. upon absorption of light energy all studied phenylphenalenones sensitise the production of singlet oxygen in polar and non-polar media. antifungal activity of these compounds towards fusarium oxysporum is enhanced in the presence of light. these results, together with the correlation of ic50 values under illumination with the qu ... | 2004 | 15239009 |
| fungus-elicited metabolites from plants as an enriched source for new leishmanicidal agents: antifungal phenyl-phenalenone phytoalexins from the banana plant (musa acuminata) target mitochondria of leishmania donovani promastigotes. | two antifungal phenyl-phenalenone phytoalexins isolated from the banana plant (musa acuminata) elicited with the fungus fusarium oxysporum, together with a methoxy derivative of one of them and two epoxide precursors of their chemical synthesis, were tested for leishmanicidal activity on leishmania donovani promastigotes and l. infantum amastigotes. drugs inhibited proliferation of both forms of the parasite with a 50% lethal concentration range between 10.3 and 68.7 micro g/ml. their lethal mec ... | 2004 | 15105102 |
| effect of phytohormones on pectate lyase activity in ripening musa acuminata. | a differential activity peak of pectate lyase (pel) was observed during ripening of banana fruits (musa acuminata harichhal) receiving different hormone treatments. exposure of fruits to 25 ppm ethylene for 24 h, as well as dipping of m. acuminata fruits in 1 mm 2,4-dichlorophenoxy acetic acid (2,4-d) for 4 h, hastened fruit ripening. both pel activity peak and climacteric peak were observed on the 4th and 10th days of treatment with ethylene and 2,4-d, respectively, compared to the 16th day in ... | 2004 | 15694279 |
| [soil arthropod communities in different successional tropical secondary forests in xishuangbanna]. | studies on the soil arthropod community structure and its seasonal variation in four types of secondary forest, including macaranga denticulate forest (md), millettia laptobotrya forest (ml), musa acuminata forest (ma) and dendrocalamus membranaceae forest (dm) in xishuangbanna showed that acari, hymenoptera (ant) and collembola were the dominant groups of soil arthropods on all study sites, but their proportions differed in different forests. for the common and rare groups of soil arthropods, t ... | 2004 | 15362621 |
| gene content and density in banana ( musa acuminata) as revealed by genomic sequencing of bac clones. | the complete sequence of musa acuminata bacterial artificial chromosome (bac) clones is presented and, consequently, the first analysis of the banana genome organization. one clone (muh9) is 82,723 bp long with an overall g+c content of 38.2%. twelve putative protein-coding sequences were identified, representing a gene density of one per 6.9 kb, which is slightly less than that previously reported for arabidopsis but similar to rice. one coding sequence was identified as a partial m. acuminata ... | 2004 | 14985976 |
| banana contains a diverse array of endogenous badnaviruses. | banana streak disease is caused by several distinct badnavirus species, one of which is banana streak obino l'ewai virus. banana streak obino l'ewai virus has severely hindered international banana (musa spp.) breeding programmes, as new hybrids are frequently infected with this virus, curtailing any further exploitation. this infection is thought to arise from viral dna integrated in the nuclear genome of musa balbisiana (b genome), one of the wild species contributing to many of the banana cul ... | 2005 | 15659772 |
| supersage combined with pcr walking allows global gene expression profiling of banana (musa acuminata), a non-model organism. | super-serial analysis of gene expression (supersage) was used to characterize, for the first time, the global gene expression pattern in banana (musa acuminata). a total of 10,196 tags were generated from leaf tissue, representing 5,292 expressed genes. forty-nine tags of the top 100 most abundantly expressed transcripts were annotated by homology to cdna or est sequences. typically for leaf tissue, analysis of the transcript profiles showed that the majority of the abundant transcripts are invo ... | 2005 | 16133315 |
| patterns of variability in the diameter of lateral roots in the banana root system. | the relative importance of root system structure, plant carbon status and soil environment in the determination of lateral root diameter remains unclear, and was investigated in this study. banana (musa acuminata) plants were grown at various moderate levels of soil compaction in two distinct experiments, in a field experiment (fe) and in a glasshouse experiment (ge). radiant flux density was 5 times lower in ge. the distribution of root diameter was measured for several root branching orders. r ... | 2005 | 16101920 |
| [establishment of embryogenic cell suspension culture and plant regeneration of edible banana musa acuminata cv. mas (aa)]. | conventional breeding for dual resistance of disease and pest of musa cultivars remains a difficult endeavor, as the plant is polyploidic and high in sterility. biotechnological techniques, eg., genetic engineering, in vitro mutation breeding, or protoplast fusion, may overcome the difficulties and improve the germplasm. establishment of a stable embryogenic cell suspension (ecs) is a prerequisite for any of the biotechnological breeding methods. in this study an embryogenic cell suspension was ... | 2005 | 15859330 |
| analysis of expressed sequence tags from musa acuminata ssp. burmannicoides, var. calcutta 4 (aa) leaves submitted to temperature stresses. | in order to discover genes expressed in leaves of musa acuminata ssp. burmannicoides var. calcutta 4 (aa), from plants submitted to temperature stress, we produced and characterized two full-length enriched cdna libraries. total rna from plants subjected to temperatures ranging from 5 degrees c to 25 degrees c and from 25 degrees c to 45 degrees c was used to produce a cold and a hot cdna library, respectively. we sequenced 1,440 clones from each library. following quality analysis and vector tr ... | 2005 | 15841358 |
| construction and characterization of a plant transformation-competent bibac library of the black sigatoka-resistant banana musa acuminata cv. tuu gia (aa). | a plant transformation-competent binary bacterial artificial chromosome (bibac) library was constructed from musa acuminata cv. tuu gia (aa), a black sigatoka-resistant diploid banana. after digestion of high-molecular-weight banana dna by hindiii, several methods of dna size selection were tested, followed by ligation, using a vector/insert molar ratio of 4:1. the library consists of 30,700 clones stored in 80 384-well microtiter plates. the mean insert size was estimated to be 100 kb, and the ... | 2005 | 15650812 |
| resolution of the structure of the allergenic and antifungal banana fruit thaumatin-like protein at 1.7-a. | the structure of a thaumatin-like protein from banana (musa acuminata) fruit, an allergen with antifungal properties, was solved at 1.7-a-resolution, by x-ray crystallography. though the banana protein exhibits a very similar overall fold as thaumatin it markedly differs from the sweet-tasting protein by the presence of a surface exposed electronegative cleft. due to the presence of this electronegative cleft, the banana thaumatin-like protein (ban-tlp) acquires a strong (local) electronegative ... | 2006 | 16085352 |
| structural characterization of lignin from leaf sheaths of "dwarf cavendish" banana plant. | dioxane lignin (dl) isolated from leaf sheaths of banana plant (musa acuminata colla var. cavendish) and in situ lignin were submitted to a comprehensive structural characterization employing spectroscopic (uv, ftir, solid state 13c cp-mas nmr, liquid state 13c and 1h nmr) and chemical degradation techniques (permanganate and nitrobenzene oxidation). results obtained showed that banana plant leaf sheath lignin is of hgs type with a molar proportion of p-hydroxyphenyl (h)/guaiacyl (g)/syringyl (s ... | 2006 | 16569050 |
| purification and characterization of pectate lyase from banana (musa acuminata) fruits. | pectate lyase (pel) has been purified by hydrophobic, cation exchange and size exclusion column chromatographies from ripe banana fruit. the purified enzyme has specific activity of 680 +/- 50 pkat mg protein(-1). the molecular mass of the enzyme is 43 kda by sds-page. the pi of the enzyme is 8 with optimum activity at ph 8.5. analysis of the reaction products by paper and anion exchange chromatographies reveal that the enzyme releases several oligomers of unsaturated galacturonane from polygala ... | 2006 | 16554075 |
| antioxidant capacity of crude extracts from clones of banana and plane species. | banana and plane are the most important fruits in world trade, behind citric plants. in this work we studied the antioxidant capacity of banana and plane varieties of fruits obtained from interspecies crossed varieties of musa acuminata and musa balbisiana, named harton plane, cavendish banana, and manzano banana. with this purpose we evaluated banana and plane crude extracts using the ferrous ion oxidation with xylenol orange method, the thiobarbituric acid method, determination of antioxidant ... | 2006 | 17201639 |
| identification of genes differentially expressed during ripening of banana. | the banana (musa acuminata, subgroup cavendish 'grand nain') is a climacteric fruit of economic importance. a better understanding of the banana ripening process is needed to improve fruit quality and to extend shelf life. eighty-four up-regulated unigenes were identified by differential screening of a banana fruit cdna subtraction library at a late ripening stage. the ripening stages in this study were defined according to the peel color index (pci). unigene sequences were analyzed with differe ... | 2007 | 16934912 |
| utility of selected non-coding chloroplast dna sequences for lineage assessment of musa interspecific hybrids. | single-copy chloroplast loci are used widely to infer phylogenetic relationship at different taxonomic levels among various groups of plants. to test the utility of chloroplast loci and to provide additional data applicable to hybrid evolution in musa, we sequenced two introns, rpl16 and ndha, and two intergenic spacers, psaa-ycf3 and peta-psbj-psbl-psbf and combined these data. using these four regions, musa acuminata colla (a)- and m. balbisiana colla (b)-containing genomes were clearly distin ... | 2007 | 17669275 |
| a bac end view of the musa acuminata genome. | musa species contain the fourth most important crop in developing countries. here, we report the analysis of 6,252 bac end-sequences, in order to view the sequence composition of the musa acuminata genome in a cost effective and efficient manner. | 2007 | 17562019 |
| protection of ultrastructure in chilling-stressed banana leaves by salicylic acid. | chilling tolerance of salicylic acid (sa) in banana seedlings (musa acuminata cv., williams 8818) was investigated by changes in ultrastructure in this study. | 2007 | 17444604 |
| molecular characterization of banana streak acuminata vietnam virus isolated from musa acuminata siamea (banana cultivar). | an isolate of banana streak virus (bsv) that does not also occur as an integrant in the musa balbisiana genome was sought in order to investigate the biological role of bsv in the evolution of either the musa genome or of the virus itself. we isolated bsv virions from a musa acuminata siamea accession from vietnam and sequenced the entire viral genome. the molecular organization is similar to that described for other bsv but slightly larger (7801 bp vs. 1611-7568 bp), and orf i has a non-convent ... | 2007 | 17431738 |
| cloning and expression of resistance gene analogs (rgas) from wild banana resistant to banana fusarium wilt. | wild banana species are essential natural gene pools for banana improvement. in this study, six rgas about 500 bp were obtained from leaves of musa acuminata, a wild banana shown to be resistant to banana fusarium wilt race 4, by pcr amplification with degenerate primers designed according to the conserved nbs motif and serine/threonine kinase domain of plant resistance (r) genes. among these rgas, the deduced amino acids of wnb1 and wnb2 contain nb-arc domain and wnb1 can be translated into pol ... | 2007 | 18349511 |
| phenalenone-type compounds from musa acuminata var. "yangambi km 5" (aaa) and their activity against mycosphaerella fijiensis. | two perinaphthenone-type compounds (1 and 2) were isolated together with four known phenylphenalenones (3-6) from the rhizomes of musa acuminata var. "yangambi km 5". the structures of the new phenalenones were assigned as 2-hydroxy-1h-phenalen-1-one (1) and 2-methoxy-1h-phenalen-1-one (2) on the basis of their spectroscopic data and were confirmed by synthesis. compounds 1 and 2 displayed significantly enhanced activity against mycosphaerella fijiensis in comparison with other phenylphenalenone ... | 2007 | 17428093 |
| highly efficient agrobacterium-mediated transformation of embryogenic cell suspensions of musa acuminata cv. mas (aa) via a liquid co-cultivation system. | a high efficient protocol of agrobacterium-mediated transformation of musa acuminata cv. mas (aa), a major banana variety of the south east asia region, was developed in this study. male-flower-derived embryogenic cell suspensions (ecs) were co-cultivated in liquid medium with agrobacterium strain eha105 harboring a binary vector pcambia2301 carrying nptii and gusa gene in the t-dna. depending upon conditions and duration of co-cultivation in liquid medium, 0-490 transgenic plants per 0.5 ml pac ... | 2007 | 17551731 |
| structural analysis of catalase from two musa accessions, fhia18 and williams, and from ravenala madagascariensis. | nucleotide sequences of catalase were obtained following amplification using specific primers and were blasted against musa acuminata catalase 2 mrna from ncbi (157418810). clustering of the amino acid sequences from ncbi was done using clustal x. the latter revealed that fhia18 catalase is more related to ravenala madagascariensis (musa relative) catalase while the williams catalase is more related to a clade containing a musa acuminata (musa ancestor) catalase from ncbi. the tertiary structure ... | 2008 | 19374128 |
| analysis of the sugar-binding specificity of mannose-binding-type jacalin-related lectins by frontal affinity chromatography--an approach to functional classification. | the jacalin-related lectin (jrl) family comprises galactose-binding-type (gjrls) and mannose-binding-type (mjrls) lectins. although the documented occurrence of gjrls is confined to the family moraceae, mjrls are widespread in the plant kingdom. a detailed comparison of sugar-binding specificity was made by frontal affinity chromatography to corroborate the structure-function relationships of the extended mjrl subfamily. eight mjrls covering a broad taxonomic range were used: artocarpin from art ... | 2008 | 18266762 |
| activity against drug resistant-tuberculosis strains of plants used in mexican traditional medicine to treat tuberculosis and other respiratory diseases. | tuberculosis (tb) kills about 3 million people per year worldwide. furthermore, tb is an infectious disease associated with hiv patients, and there is a rise in multidrug-resistant tb (mdr-tb) cases around the world. there is a need for new anti-tb agents. the study evaluated the antimycobacterial activity of nine plants used in mexican traditional medicine to treat tuberculosis and other respiratory diseases. nasturtium officinale showed the best activity (mic = 100 microg/ml) against the sensi ... | 2008 | 17726732 |
| analysis of non-tir nbs-lrr resistance gene analogs in musa acuminata colla: isolation, rflp marker development, and physical mapping. | many commercial banana varieties lack sources of resistance to pests and diseases, as a consequence of sterility and narrow genetic background. fertile wild relatives, by contrast, possess greater variability and represent potential sources of disease resistance genes (r-genes). the largest known family of plant r-genes encode proteins with nucleotide-binding site (nbs) and c-terminal leucine-rich repeat (lrr) domains. conserved motifs in such genes in diverse plant species offer a means for iso ... | 2008 | 18234103 |
| ein3-like gene expression during fruit ripening of cavendish banana (musa acuminata cv. grande naine). | ethylene signal transduction initiates with ethylene binding at receptor proteins and terminates in a transcription cascade involving the ein3/eil transcription factors. here, we have isolated four cdnas homologs of the arabidopsis ein3/ein3-like gene, ma-eils (musa acuminata ethylene insensitive 3-like) from banana fruit. sequence comparison with other banana eil gene already registered in the database led us to conclude that, at this day, at least five different genes namely ma-eil1, ma-eil2/a ... | 2008 | 18346078 |
| differential transcriptional regulation of banana sucrose phosphate synthase gene in response to ethylene, auxin, wounding, low temperature and different photoperiods during fruit ripening and functional analysis of banana sps gene promoter. | sucrose phosphate synthase (sps) (ec 2.3.1.14) is the key regulatory component in sucrose formation in banana (musa acuminata subgroup cavendish, cv giant governor) fruit during ripening. this report illustrates differential transcriptional responses of banana sps gene following ethylene, auxin, wounding, low temperature and different photoperiods during ripening in banana fruit. whereas ethylene strongly stimulated sps transcript accumulation, auxin and cold treatment only marginally increased ... | 2008 | 18830708 |
| [development and application of est-derived ssr markers for bananas (musa nana lour.)]. | a total of 2,284 banana unigene sequences were mined, resulting in the identification of 122 ssrs in 110 sequences. among them, there were 37 motifs the overall average length of ssrs was 20 bp. the trinucleotide repeats appeared to be the most abundant ssrs (47.6%), followed by dinucleotide repeats (33.1%), whereas the tetranucleotide repeats were less abundant. the rich repeats ag and agg were predominant, accounting for 75.7% and 36% in dri- and tri-nucleotide repeats respectively. a total of ... | 2008 | 18779139 |
| identification of cytoplasmic ancestor gene-pools of musa acuminata colla and musa balbisiana colla and their hybrids by chloroplast and mitochondrial haplotyping. | cytoplasmically inherited characters such as resistance to viral and fungal diseases, determination of starch types, crop yield, resistance to low or high temperature often contribute to the advantageous phenotypic traits of plants. in the present study, our goal was to elucidate the genealogy of cytoplasmic genomes chloroplast and mitochondria in banana. banana breeding is rather complicated because of the low fertility and mostly unknown origin of the edible cultivars, therefore, knowledge on ... | 2008 | 18762903 |
| characterization of transcriptional profiles of ma-acs1 and ma-aco1 genes in response to ethylene, auxin, wounding, cold and different photoperiods during ripening in banana fruit. | the ripening-specific genes ma-acs1 (musa acuminata acc synthase1) and ma-aco1 (m. acuminata acc oxidase 1) are regulated in response to a wide variety of factors. here, we have studied the differential transcript accumulation pattern and protein levels of ma-acs1 and ma-aco1 genes in response to ethylene, auxin, wounding and low temperature in preclimacteric banana fruit. we have shown that exogenous application of ethylene and auxin induced the expression of ma-acs1, while ma-aco1 showed margi ... | 2008 | 18554749 |
| genomes, diversity and resistance gene analogues in musa species. | resistance genes (r genes) in plants are abundant and may represent more than 1% of all the genes. their diversity is critical to the recognition and response to attack from diverse pathogens. like many other crops, banana and plantain face attacks from potentially devastating fungal and bacterial diseases, increased by a combination of worldwide spread of pathogens, exploitation of a small number of varieties, new pathogen mutations, and the lack of effective, benign and cheap chemical control. ... | 2008 | 18544928 |
| involvement of a banana mads-box transcription factor gene in ethylene-induced fruit ripening. | to investigate the regulation of mads-box genes in banana (musa acuminata l. aaa group cv. brazilian) fruit development and postharvest ripening, we isolated from banana fruit a mads-box gene designated mumads1. amino acid alignment indicated mumads1 belongs to the agamous subfamily, and phylogenetic analysis indicates that this gene is most similar to class d mads-box genes. reverse transcriptase-polymerase chain reaction (rt-pcr) analysis showed that mumads1 is expressed in the stamen and pist ... | 2009 | 18820933 |
| fluorescent chlorophyll catabolites in bananas light up blue halos of cell death. | breakdown of chlorophyll is a major contributor to the diagnostic color changes in fall leaves, and in ripening apples and pears, where it commonly provides colorless, nonfluorescent tetrapyrroles. in contrast, in ripening bananas (musa acuminata) chlorophylls fade to give unique fluorescent catabolites (fccs), causing yellow bananas to glow blue, when observed under uv light. here, we demonstrate the capacity of the blue fluorescent chlorophyll catabolites to signal symptoms of programmed cell ... | 2009 | 19805212 |
| accumulation of soluble sugars in peel at high temperature leads to stay-green ripe banana fruit. | bananas (musa acuminata, aaa group) fail to develop a yellow peel and stay green when ripening at temperatures >24 degrees c. the identification of the mechanisms leading to the development of stay-green ripe bananas has practical value and is helpful in revealing pathways involved in the regulation of chlorophyll (chl) degradation. in the present study, the chl degradation pathway was characterized and the progress of ripening and senescence was assessed in banana peel at 30 degrees c versus 20 ... | 2009 | 19700495 |
| development and assessment of diversity arrays technology for high-throughput dna analyses in musa. | diversity arrays technology (dart) is a dna hybridisation-based molecular marker technique that can detect simultaneously variation at numerous genomic loci without sequence information. this efficiency makes it a potential tool for a quick and powerful assessment of the structure of germplasm collections. this article demonstrates the usefulness of dart markers for genetic diversity analyses of musa spp. genotypes. we developed four complexity reduction methods to generate dart genomic represen ... | 2009 | 19693484 |
| chemical composition and physicochemical properties of green banana (musa acuminata x balbisiana colla cv. awak) flour. | flour was prepared from peeled and unpeeled banana awak abb. samples prepared were subjected to analysis for determination of chemical composition, mineral, dietary fibre, starch and total phenolics content, antioxidant activity and pasting properties. in general, flour prepared from unpeeled banana was found to show enhanced nutrition values with higher contents of mineral, dietary fibre and total phenolics. hence, flour fortified with peel showed relatively higher antioxidant activity. on the ... | 2009 | 19449278 |
| effect of banana pulp and peel flour on physicochemical properties and in vitro starch digestibility of yellow alkaline noodles. | the present study describes the utilization of banana--cavendish (musa acuminata l., cv cavendshii) and dream (musa acuminata colla. aaa, cv 'berangan')--pulp and peel flours as functional ingredients in yellow alkaline noodles. noodles were prepared by partial substitution of wheat flour with ripe banana pulp or peel flours. in most cases, the starch hydrolysis index, predicted glycaemic index (pgi) and physicochemical properties of cooked noodles were affected by banana flour addition. in gene ... | 2009 | 19757248 |
| musa acuminata (del monte banana) lectin is a fructose-binding lectin with cytokine-inducing activity. | a homodimeric, fructose-binding lectin was isolated from del monte bananas by using a protocol that involved ion-exchange chromatography on deae-cellulose and sp-sepharose, and gel filtration by fast protein liquid chromatography on superdex 75. not only fructose, but also glucose, mannose, rhamnose and glucosamine could inhibit the lectin. the n-terminal amino acid sequence of its identical 15-kda subunits was similar to lectins from other musa species except for the deletion of the n-terminal ... | 2009 | 19195858 |
| phenolics and antioxidant properties of fruit pulp and cell wall fractions of postharvest banana (musa acuminata juss.) cultivars. | banana fruits are important foods, but there have been very few studies evaluating the phenolics associated with their cell walls. in the present study, (+) catechin, gallocatechin, and (-) epicatechin, as well as condensed tannins, were detected in the soluble extract of the fruit pulp; neither soluble anthocyanidins nor anthocyanins were present. in the soluble cell wall fraction, two hydroxycinnamic acid derivatives were predominant, whereas in the insoluble cell wall fraction, the anthocyani ... | 2010 | 20553046 |
| citric acid production by koji fermentation using banana peel as a novel substrate. | the growing demand for citric acid and the current need for alternative sources have encouraged biotechnologists to search for novel and economical substrates. koji fermentation was conducted using the peels of banana (musa acuminata) as an inexpensive substrate for the production of citric acid using aspergillus niger. various crucial parameters that affect citric acid production such as moisture content, temperature, ph, inoculum level and incubation time were quantified. moisture (70%), 28 de ... | 2010 | 20219361 |