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[pancreatic oncofetal antigen (poa)--its characterization and the result of enzyme immunoassay].our studies on the partial purification, characterization and clinical application of pancreatic oncofetal antigen (poa) are presented. this antigen was purified from fetal pancreas and migrated in beta-region electrophoretically. its molecular weight was estimated approximately 80 x 10(4) daltons on gel filtration. poa prepared by us was different from either nishida's poa biochemically or shimano's pcaa immunologically. enzyme immunoassay of poa was established by conjugating with anti-poa wit ...19836349543
vincristine, doxorubicin and mitomycin (vam) in patients with advanced breast cancer previously treated with cyclophosphamide, methotrexate and fluorouracil (cmf). a clinical trial of the piedmont oncology association (poa).thirty-six evaluable patients with advanced breast cancer who had failed prior cmf therapy [15 (42%) as adjuvant treatment and 21 (58%) for advanced disease] were treated with a combination of vincristine, doxorubicin, and mitomycin (vam). there was one cr and 10 pr, giving a response rate of 31% (p, 95% confidence interval, 15%-47%). response was not significantly related to age, performance status, disease-free interval, dominant site of disease, number of sites of disease, or estrogen recepto ...19836354509
stimulation with estrogen and progesterone of luteinizing hormone (lh)-releasing hormone release from perifused adult female rat hypothalami: correlation with the lh surge.the effects of estradiol benzoate (eb) and progesterone (p) in vivo and p in vitro on lhrh release from perifused preoptic area-medial basal hypothalamus (poa-mbh) tissue fragments were assessed. in the first series of experiments, ovariectomized female rats were given either 10 or 30 micrograms eb on day 0, followed by either oil or 5 mg p at 1000 h on day 2. rats were killed at 2-h intervals after p or oil injection, and the poa-mbh from these rats were incubated in a perifusion system. lhrh r ...19846360665
[identification of allergens in 5 grasses using crossed radioimmunoelectrophoresis (crie)].using crossed radioimmunoelectrophoresis ( crie ) aqueous extracts from pollen of phleum pratense , lolium perenne, poa pratensis , festuca pratensis and alopecurus pratensis were investigated for allergen composition. we detected between 24 and 32 antigens. employing sera from 11 patients with well established hay fever, ige binding could be demonstrated in 15 out of 28 antigens in phleum pratense , 13 out of 32 in lolium perenne, 14 out of 26 in poa pratensis , 12 out of 24 festuca pratensis a ...19846233859
residues in the fat of ewes grazing on soil contaminated with halogenated hydrocarbons.three halogenated hydrocarbon compounds were applied to the surface of each of two .57 ha bluegrass plots in october. each plot was stocked with 10 mature ewes 6 mo after application. supplemental feed was not offered. five ewes remained on the plots for 180 d, whereas the other five were removed and replaced at 60-d intervals. average soil residues for the period of grazing were 13.3, 3.8, 29.3 and 32.8 mg/m2 hcb (hexachlorobenzene), dde [1,1-dichloro-2,2-bi(p-chlorophenyl)ethylene], ddt [1,1,1 ...19826294042
[evaluation of pancreatic oncofetal antigen (poa) in the diagnosis of pancreatic cancer].pancreatic oncofetal antigen (poa) is considered to be an oncofetal antigen for human pancreas, and its measurement seems to be useful in the diagnosis of pancreatic cancer. in this study, poa, cea, ferritin, bmg (beta 2 microglobulin) and afp either in sera in pancreatic juice were measured in patients with pancreatic cancer, chronic pancreatitis and other various diseases, and their diagnostic value was comparatively evaluated. poa showed the highest sensitivity for pancreatic cancer compared ...19836349542
steroid-monoamine feedback interactions in discrete brain regions using as a model the monosodium glutamate (msg)-lesioned rat.the present studies examine the effects of neonatal treatment with monosodium glutamate (msg) on dopamine (da), 5-hydroxytryptamine (5-ht) and norepinephrine (ne) metabolism in discrete brain regions and correlate them with steroid receptor kinetics in the anterior pituitary (pit), preoptic hypothalamus (poa) and caudal hypothalamus (hyp), and with steroid negative and positive feedback effects on luteinizing hormone (lh) secretion. substantial decreases in the neuronal activity of all three ami ...19846369057
preoptic area brain grafts in hypogonadal (hpg) female mice abolish effects of congenital hypothalamic gonadotropin-releasing hormone (gnrh) deficiency.normal fetal preoptic area (poa), a site of gnrh production, was implanted into the third ventricle of adult female hypogonadal (hpg) mice. when the grafts were successful, the mice (genetically deficient in hypothalamic gnrh) responded with vaginal opening, cornified vaginal cells, ovarian and uterine development, and increased pituitary fsh content and plasma lh concentrations. similar results were obtained with fetal poa tissue, whether derived from males or females. two of four hpg mice with ...19846370670
axonal projections and peptide content of steroid hormone concentrating neurons.the axonal projections of cell groups containing the most dense collections of steroid hormone concentrating cells have been demonstrated with retrograde neuroanatomical tracing methods. horseradish peroxidase revealed large numbers of neurons in ventrolateral ventromedial nucleus (vl-vm) which project to dorsal midbrain. wheat germ agglutinin (immunocytochemical recognition method) revealed large numbers of neurons in medial basal hypothalamus (mbh) and particular subdivisions of paraventricula ...19846384952
drinking behavior is modulated by cns administration of opioids in the rat.while opiate antagonists have been shown to reliably attenuate drinking following both central and peripheral administration, relatively few data exist on the effects of agonist agents on this behavior. to address this issue, two opiate agonists, morphine sulfate, a mu agonist, and [d-ala2, d-leu5]-enkephalin (dadle), a semi-synthetic delta analog of a delta agonist, were administered into several cns sites in rats. there was a dose-related, naloxone-reversible reduction of water intake followin ...19846385841
high-dose cyclophosphamide and 5-fluorouracil versus vincristine, doxorubicin, and cyclophosphamide in advanced carcinoma of the breast. a phase iii study of the piedmont oncology association (poa).forty-nine patients with advanced carcinoma of the breast who had received no prior chemotherapy were randomized to receive either high-dose cyclophosphamide (c) 1250 mg/m2 intravenously on day 1 and 5-fluorouracil (f) 600 mg/m2 intravenously on days 1 through 5 (cf), or vincristine (v) 1.5 mg/m2, doxorubicin (a) 50 mg/m2 and cyclophosphamide (c) 500 mg/m2 (vac), all intravenously on day 1. both regimens were repeated at 3-week intervals. nine of 25 patients (36%) treated with cf and ten of 21 p ...19846388802
telencephalic and preoptic areas integrate sexual behavior in hime salmon (landlocked red salmon, oncorhynchus nerka): results of electrical brain stimulation experiments.various patterns of sexual behavior were evoked in freely swimming hime salmon by electrical stimulation of specific loci in the telencephalon and the preoptic area (poa) using chronically implanted electrodes. furthermore, co-ordinated sexual behavior corresponding to stages of the natural spawning sequence was elicited from some of these brain regions. these results suggest that (1) sexual behavior is integrated in specific parts of the telencephalon and poa, and (2) within these regions there ...19846393162
differential distribution of the pancreatic cancer-associated antigen (pcaa) and pancreatic tissue antigen (paa) in pancreatic and gastrointestinal cancer tissues.pcaa, a glycoprotein antigen fractionated from the ascites fluid of a patient with pancreatic cancer and sharing an identical immunogenicity with gelder's poa, and paa, a novel pancreatic tissue antigen, were studied immunohistologically. serial paraffin sections were prepared from surgical specimens of 11 cases of pancreatic cancer, 15 of gastric cancer, 12 of colonic cancer, and 2 of gallbladder cancer; these were then subjected to immunofluorescence and immunoperoxidase stainings. in noncance ...19836367596
clinical application of the enzyme immunoassay for pancreatic oncofetal antigen.pancreatic oncofetal antigen (poa) purified by us has been detected in sera of patients with carcinoma of the pancreas by the micro-ouchterlony method. in an attempt to improve the sensitivity and clinical usefulness of the serum poa assay, we established an enzyme immunoassay for poa and reported the results with this method. in this study, we investigated serum poa levels in pancreatic cancer and other diseases. the tissue localization of this poa in the pancreas was also studied. for the esta ...19836367597
a pancreatic oncofetal antigen (poa): its characterization and application for enzyme immunoassay.we investigated the usefulness of enzyme immunoassay (eia) for pancreatic oncofetal antigen (poa). the crude poa isolated from poa-positive ascitic fluid of patients with pancreatic cancer was injected into rabbits to raise anti-poa serum. the adsorbed antiserum was used for eia as anti-poa serum. for the establishment of eia system for poa, anti-poa-fab' fragment was conjugated to beta-d-galactosidase from escherichia coli. normal subjects (205 controls) and 132 patients (47 with pancreatic can ...19846367984
purification and characterization of a pancreatic cancer-associated antigen (pcaa) from normal colonic mucosa.the same antigenic substance as pancreatic cancer-associated antigen (pcaa), sharing an identical immunogenicity with gelder's poa, was isolated and purified from normal colonic mucosa. purified colonic pcaa (pcaac) is a glycoprotein with a molecular weight of approximately 600,000; it consists of 30% carbohydrates and 70% peptides, and appears to be an n-glycosidic glycoprotein. antiserum raised against purified pcaac showed one fused line with our original anti-pcaa antiserum to ascites fluid ...19836422827
effects of histamine antagonists injected in the preoptic-anterior hypothalamic area on the prolactin surge induced by estrogen in ovariectomized rats.thirty five nmoles of pyrilamine (a h1-histamine antagonist) or metiamide (a h2-histamine antagonist) in 1 microliter of saline solution were injected into the preoptic-anterior hypothalamic area (poa-aha) of estrogen-treated ovariectomized rats. forty-eight hours later animals were bled between 15:00 and 20:00 hr. prolactin (prl) plasma levels were measured by ria. the anti-histamine treatment altered the prl surge normally observed 72 hr after the estrogen injection in these animals under this ...19846424868
enzymatic synthesis of phenoxymethylpenicillin using erwinia aroideae enzyme.enzymatic synthesis of phenoxymethylpenicillin from 6-aminopenicillanic acid and phenoxyacetic acid methyl ester was attempted by using partially purified alpha-acylamino-beta-lactam acylhydrolase i (alahase i) enzyme from erwinia aroideae nrrl b-138. the reaction rates were carefully followed by determination of 6-aminopenicillanic acid (6-apa), phenoxymethylpenicillin (pnv), phenoxyacetic acid (poa), phenoxyacetic acid methyl ester (pom), and phenoxyacetylglycine (pog) using high performance l ...19846438038
changes in hypothalamic and pituitary content of immunoreactive alpha-melanocyte-stimulating hormone during the gestational and postpartum periods in the rat.alpha-melanocyte-stimulating hormone (alpha-msh) was measured in the mediobasal hypothalamus (mh), median eminence (me), preoptic-suprachiasmatic area (poa-scn), anterior (al), and posterior lobes (pl) of the pituitary gland during the gestational and postpartum periods in the rat. the content of alpha-msh in the mh and poa-scn compared to estrous levels was lower during the later days of gestation and decreased further in the mh during lactation in association with the elevated plasma prolactin ...19846483865
antagonism of central estrogen action by intracerebral implants of tamoxifen.in order to determine the neural site(s) of estradiol (e2) priming of receptive behavior in female long-evans rats, we attempted to inhibit the behavioral effects of peripheral injections of e2 by administering the e2 antagonist tamoxifen (tx) to particular brain regions. crystalline tx was administered unilaterally or bilaterally via 28-gauge cannulae into the ventromedial hypothalamic nucleus (vmn), the preoptic area (poa), or the interpeduncular region (ip) 1 hr prior to the first of three da ...19846489941
[clinical evaluation of enzyme immunoassay for a pancreatic oncofetal antigen (poa)]. 19846398349
chronic hyperprolactinemia causes progressive changes in hypothalamic dopaminergic and noradrenergic neurons.studies were undertaken to evaluate the effects of chronic hyperprolactinemia (hyp) on catecholamine concentrations and turnover rates in brain regions of the female rat. hyp was induced by inoculation of tissue derived from the prolactin secreting mttw15 tumor. when serum prolactin (prl) levels were moderately elevated, medial basal hypothalamus (mbh) dopamine (da) turnover was enhanced and da concentrations were moderately reduced. later, as serum prl levels increased to greater than 10 microg ...19846478222
influence of ascending reticular activating system on preoptic neuronal activity.there is evidence to suggest that the reticular activating system may have an influence on the neurons of the preoptic area (poa). we studied the responses of poa neurons and their relation to the cortical eeg, on stimulation of the midbrain reticular formation (mrf) at various frequencies, in unanesthetized, encéphale isolé cats. stimulation of the mrf at high frequency produced inhibition of the firing rate in a majority of the responsive neurons of the poa. stimulation of the mrf with lower f ...19846479280
pharmacological and anatomical aspects of cholinergic activation of female sexual behavior.forebrain infusion of cholinergic agonists activated the sexual response, lordosis, in ovariectomized female rats that had been primed with a low dose of estrogen. carbachol, an agonist with both muscarinic and nicotinic properties, and oxotremorine, an agonist with a primarily muscarinic action, produced dose-related increases in the frequency of lordosis elicited by stimulus male rats. this facilitation of lordosis was prevented when females were pretreated systemically with atropine or scopol ...19846494300
suppression of sexual behavior and localization of [3h] puromycin after intracranial injection in the rat.bilateral intracranial injections of puromycin (10 micrograms/0.5 microliter vehicle) into the preoptic area (poa) of steroid-primed ovariectomized rats resulted in a significant decrease in the lordosis response when compared to saline treated controls. to determine the extent of puromycin diffusion after intracranial injection, [3h] puromycin was injected into the right poa of ovariectomized rats. neural regions anterior, posterior and superior to the poa, and the right and left poa were asses ...19846514830
brain catecholamine content during the estrous cycle and in steroid-primed rats.norepinephrine (ne) and dopamine (da) levels were measured in several estrogen concentrating brain regions over the estrous cycle and in steroid-primed ovariectomized rats under experimental conditions used to study sexual receptivity. norepinephrine content in the ventromedial hypothalamus (vmh), lateral septum (ls) and medial preoptic area (poa) varied during the estrous cycle. the highest ne levels were found during metestrus in these brain regions. in the ovariectomized rat, ne levels were i ...19846539636
a field-potential study of centripetal and centrifugal connections of the olfactory bulb in the carp, cyprinus carpio (l).the projection areas of mitral cell axons in the lateral (lot) and medial (mot) olfactory tracts and the pathways of two groups of centrifugal fibers (with fast and slow conduction velocities) to the olfactory bulb were studied in the carp using the field potential method. mitral cell axons of the lot and those of the mot projected to the overlapping areas in the bilateral telencephalon and preoptic area (poa), the contralateral mot, and the nucleus posterior tuberis (npt) in the diencephalon. h ...19846498513
disruption of ongoing maternal responsiveness in rats by central administration of morphine sulfate.the ability of central morphine administration to disrupt established maternal responsiveness in rats was examined. studies focused on the direct administration of morphine sulfate (m) to the preoptic area (poa), a region known to be involved in the expression of maternal behavior in this species. in the first experiment, crystalline m was administered via bilateral 28 g cannulae to the poa or ventromedial hypothalamus (vmh) of 31 ovariectomized, estrogen-primed, nulliparous, pup-induced, matern ...19846540617
effects of small medial preoptic lesions on estrous cycles and receptivity in female rats.the effects of small lesions in the preoptic area (poa) of female rats on estrous cycles, lh responsiveness, and receptivity were studied. regularly cycling rats received lesions confined to either the periventricular portion or the ventral portion of the rostral preoptic area, or a combination of these two sites. nine animals showed persistent vaginal cornification (pvc) following lesion placement. all of these had lesions in the periventricular preoptic area. in contrast, five of six animals w ...19846540883
progesterone-like effects of estradiol on reproductive behavior and hypothalamic progestin receptors in the female rat.during the rat estrous cycle, estradiol (e2) and progesterone (p) synergize to activate reproductive behavior. however, receptivity and proceptivity can be elicited by e2 alone in ovariectomized (ovx) animals, particularly when e2 doses are high. the purpose of this study was to determine the neuroendocrine mechanism by which e2 elicits p-dependent reproductive behavior. adult ovx females received estrogen treatment for 72 h, which consisted of 5 mm silastic capsules containing 100% e2 or 10% e2 ...19846540375
dissociation of prolactin and lh release responses after stimulation within the preoptic-suprachiasmatic region in male rats.the relative efficiency of unilateral electrical stimuli applied within the preoptic-suprachiasmatic region (poa-sch) to induce the release of prolactin and/or lh was studied in male rats under pentobarbital anesthesia. it was observed that unilateral stimulation with 10 hz, 1.0 msec width pulses applied in the ventral half of the poa-sch resulted primarily in the release of prolactin rather than lh. fifty hz stimuli applied at this same locus resulted also in lh release. it is concluded that th ...19846543186
changes in concentrations of estradiol nuclear receptors in the preoptic area, medial basal hypothalamus, amygdala, and pituitary gland of middle-aged and old cycling rats.this study determined whether the biochemical characteristics of the estradiol (e2) nuclear receptor located in three different brain areas and the pituitary gland (pit) change in aging rats undergoing declining reproductive function. to measure maximal e2 nuclear receptor concentrations, groups of young (3-4 months old), middle-aged (8-11 months old), and old (16-18 months old) cycling rats were injected iv with e2 to translocate maximally cytosolic receptors into the nucleus. one hour later th ...19846537808
differentiation of the sexually dimorphic nucleus in the preoptic area of the rat brain is inhibited by postnatal treatment with an estrogen antagonist.the volume of the sexually dimorphic nucleus in the preoptic area (sdn-poa) of the rat brain is several fold larger in adult male rats that in adult females. this sex difference in brain structure was previously shown to develop under the influence of androgenic and estrogenic hormones during the perinatal period. we here report that treatment of newborn male and female rats with the estrogen antagonist tamoxifen significantly inhibited growth and differentiation of the sdn-poa in both sexes and ...19846610133
progesterone in the ventromedial hypothalamus of ovariectomized, estrogen-primed rats inhibits subsequent facilitation of estrous behavior by systemic progesterone.the putative neural target sites of progesterone's (p) inhibitory influence on estrous behavior were re-examined utilizing intracranial hormone implants. subjects were estrogen-primed ovariectomized, long-evans rats, and all were outfitted with permanent indwelling guide cannulae aimed for the ventromedial hypothalamus (vmh), preoptic area (poa), or midbrain. in a series of 4 weekly testing paradigms, the ability of blank or p-filled implants to both facilitate estrous responsiveness and to inte ...19846538109
immunocytochemical identification of the mesotocin- and vasotocin-producing systems in the brain of temperate and desert lizard species and their modifications by cold exposure.using immunofluorescent techniques, mesotocin (mt) and vasotocin (vt) neurosecretory systems were identified in the brain of different lizards: one temperate species living in southern france and three desert species coming from southern algeria. in the four species, mt and vt were shown to be synthesized in specific neurons located in the anterior preoptic area (poa), the supraoptic (son), paraventricular (pvn), and ventromedial (vmn) nuclei. the neurosecretory axons of the poa neurons terminat ...19836628979
studies on the control of prostaglandin production by the hypothalamus in relation to lh release in the rat.prostaglandin (pg) and thromboxane (tx) synthesis by homogenates of the hypothalamus of rats has been measured in relation to the preovulatory surge of lh. prostaglandin and tx production by the median eminence (me) was five to ten times higher than that by the anterior hypothalamus/preoptic area (ah-poa). prostaglandin e2 and pgf2 alpha were the major prostaglandins synthesized by the me and ah-poa respectively. during the 4-day oestrous cycle, the pg- and tx-synthesizing capacity of the me sho ...19846593408
anti-muricide mechanisms of chlorpromazine and imipramine in ob rats: adrenoceptors and hypothalamic functions.chlorpromazine (cpz) microinjected into the posterior part of the lateral hypothalamus (p-lh) and the lateral preoptic area (1-poa) of ob rats shows anti-muricide and cataleptogenic effects as well as a drowsy pattern in cortical eeg, while imipramine (imp) injected into p-lh shows anti-muricide and cataleptogenic effects without change of cortical eeg. the present study examined the effects of drugs injected into p-lh and 1-poa on muricide, catalepsy and cortical and limbic eeg. cpz (20, 50 mic ...19846541343
synaptic changes in the hypothalamus of the prepuberal female rat administered estrogen.subcutaneous administration of estradiol benzoate (eb) to prepuberal female rats can advance vaginal opening, phasic pituitary gland luteinizing hormone (lh) secretion, and ovulation, presumably through a neural mechanism. this study investigated whether these effects are associated with changes in synaptic profiles in the arcuate nucleus of the hypothalamus (arc) and the preoptic area (poa). twenty-five-day-old female rats were administered eb, eb followed by progesterone on day 27, or oil vehi ...19836613905
uptake of serotonin and norepinephrine in hypothalamic and limbic brain regions during the estrous cycle and the effect of neurotoxin lesions on estrous cyclicity.the temporal pattern of hypothalamic and limbic aminergic activity during the estrous cycle has been measured by changes in the serotonergic and noradrenergic neuronal uptake during the regular estrous cycle. significant changes (p = 0.006, 0.01) in the uptake of 3h 5-ht and 3h ne were recorded in the suprachiasmatic nuclei (scn) at 1200 hours on proestrus. significant changes (p = 0.01, 0.018) in the uptake of 3h 5-ht in the preoptic area (poa) were found at 1200 hours on both diestrus and proe ...19836683584
prolactin and lh release induced by kainic acid administration within the preoptic-suprachiasmatic region in behaving male rats.adult male rats were chronically implanted with a multiple metal cannula assembly to allow for the administration of kainic acid within the poa-sch region. kainic acid was applied in its solid form at two dose levels: 2 micrograms and 4 micrograms. a chronic heart cannula implanted through the external jugular vein allowed for sequential blood sampling. animals were subjected to two different regimes, with 2-3 days difference, one in which blood sampling took place without kainic acid administra ...19836641886
effects on male sex behavior and preoptic dopamine neurons of hyperprolactinemia induced by mttw15 pituitary tumors.we have earlier reported that dopamine (da) activity in the preoptic anterior hypothalamic (poa-ah) region of castrated rats is inhibited by testosterone and accelerated by prl. these results suggested that dopaminergic neurons may play an important role in male copulatory behavior, particularly in the attenuation of sex behavior reported to be associated with hyperprolactinemia. we have now examined the effects of severe hyperprolactinemia on the poa-ah da activity in association with any modif ...19836641626
preoptic area unit activity during sleep and wakefulness in the cat.the spontaneous discharge of 86 preoptic area (poa) neurons was recorded extracellularly in chronically prepared cats during wakefulness (w), slow-wave sleep (sws), and rem sleep. of these, the percentage of units exhibiting maximal discharge rates in sws and rem sleep (84%) was significantly greater than that of those exhibiting a maximal discharge rate in w (16%). furthermore, those neurons that discharged rapidly in sleep (fast units) generally had a reduced discharge rate in w. sixteen of th ...19846692872
changes in concentration of hypothalamic cytosolic progestin receptors at the initiation of pseudopregnancy in rats.pseudopregnancy (psp) can be induced by a single injection of progesterone in cyclic rats and the administration of anti-progesterone serum can block the establishment of psp in cervically stimulated rats. to further investigate neuroendocrine mechanisms for the initiation of either psp or prolactin (prl) surges in connection with the neurotropic action of progesterone, cytosolic progestin receptors (prc) were identified and measured in preoptic (poa) and basal hypothalamic (bh) areas. chronolog ...19836686522
extrahypothalamic control of daily surges of prolactin secretion in pseudopregnant rat.the role of the limbic forebrain structures in controlling twice daily surges of prolactin (prl) induced by cervical stimulation was investigated after acute or chronic deafferentation of the limbic forebrain afferents to the hypothalamus in rats. the preoptic area-roof section (poa-rs), which interrupted the rostral limbic afferents at the dorsal level of the anterior commissure, induced pseudopregnancy (psp) and initiated the same nocturnal prl surges as those initiated by the cervical stimula ...19836686520
development of steroid-receptor systems in guinea pig brain. ii. cytoplasmic progestin receptors.the development of the cytoplasmic progestin receptor (cpr) system in male and female guinea pigs was examined to determine if insensitivity to the lordosis promoting activity of estrogen-progestin treatment in adult males and neonatal guinea pigs is correlated with alterations in this receptor system. gonadectomized neonatal (4-6 days old) and adult (50-65 days old) guinea pigs of both sexes were injected with estradiol benzoate (eb) and killed 40 h later. cprs were measured in the hypothalamus ...19846722575
development of steroid-receptor systems in guinea pig brain. i. cytoplasmic estrogen receptors.the development of the cytoplasmic estrogen receptor (cer) system in male and female guinea pigs was examined to determine whether attainment of sensitivity to estrogen action related to lordosis behavior is correlated with increases in brain cer concentrations. ovariectomized neonatal (7 days old) guinea pigs failed to display lordosis after priming with 10 micrograms estradiol benzoate (eb) and 0.5 mg progesterone (p). the percentage of ovariectomized steroid-treated females displaying lordosi ...19846722583
effects of season and sex on the distribution of cytosolic estrogen receptors within the brain and the anterior pituitary gland of sheep.this study was undertaken to evaluate the effects of season and sex on the distribution of cytosolic estradiol-17 beta receptors (e2-receptors) within multiple regions of the ovine brain and the anterior pituitary gland. our data confirm the existence of a saturable, high-affinity receptor specific for estradiol-17 beta (e2) in the hypothalamus and anterior pituitary gland. the concentrations of e2-receptors in tissues from castrated ewes and rams killed in december (breeding season) and may ( n ...19846733200
pre- and postnatal influence of testosterone propionate and diethylstilbestrol on differentiation of the sexually dimorphic nucleus of the preoptic area in male and female rats.the volume of the sexually dimorphic nucleus in the preoptic area (sdn-poa) of the rat brain is several fold larger in males than in females. when female rats were treated pre- and postnatally with testosterone propionate (tp) or with diethylstilbestrol (des) they became anovulatory and their sdn-poa developed equivalent in size to that of normal males. identical treatment of male rats resulted in deficient testicular development, but had no influence on sdn-poa volume. the results indicate that ...19846733514
lesions of the medial preoptic area prevent the facilitation of maternal behavior produced by amygdala lesions.in the present study we tested the hypothesis that the facilitation of maternal behavior produced by amygdala lesions is mediated through the medial preoptic area. different groups of animals sustained lesions of the corticomedial amygdala (group am), the medial preoptic area (group mpoa), both regions (group am-mpoa), the corticomedial amygdala and non mpoa control sites (group am-cx), and control sites (groups lc or sc). animals were tested for their maternal responses to foster pups presented ...19836657771
a functional dimorphism in the response of the hypothalamic-pituitary axis of prepubertal rats to steroid treatment.in the present experiment we examined the circadian neural luteinizing hormone releasing hormone (lhrh) and serum luteinizing hormone (lh) response of prepubertal male and female rats under varying steroidal manipulations (intact, castrate, castrate + estradiol 17 beta [e2] + oil and castrate + e2 + progesterone[p]). prepubertal males demonstrated greater and acyclic lhrh concentrations in both the medial basal hypothalamus (mbh) and preoptic-suprachiasmatic regions (poa-sch) irrespective of ste ...19826751417
converging visual and somatic sensory cortical input to the intraparietal sulcus of the rhesus monkey.a cyto- and myeloarchitectonic study reveals the presence of a distinct cortical zone ("area poa") in the lower bank of the intraparietal sulcus of the rhesus monkey. using both autoradiographic and silver impregnation techniques, an analysis of cortical connections shows two overlapping projections to this sulcal zone. these come from (1) the middle portion of the preoccipital gyrus (area oa) and (2) the rostral inferior parietal lobule (area pf).19806769545
courtship stimulates aromatase activity in preoptic area of brain in male ring doves.aromatase activity was quantified in vitro in different regions of the basal forebrain of male ring doves at various times after the initiation of courtship. by 24 h after the introduction of a female dove into the cage of a male, there was a significant, nearly two-fold, increase in aromatase activity which was confined to the ventromedial preoptic area (vm-poa) where enzyme activity was concentrated. this increase in aromatase activity, which was sustained through day 5 of courtship, presumabl ...19846745192
effect of estrogen on lipoprotein lipase activity and cytoplasmic progestin binding sites in lean and obese zucker rats.injections of 5 micrograms estradiol benzoate (eb) for 5 days resulted in decreases in the rate of body weight gain in both lean (fafa) and obese (fafa) zucker rats. eb administration also resulted in significant induction of cytoplasmic progestin binding sites in both hypothalamic-preoptic area (h-poa) and adipose tissues from rats of both genotypes. however, eb treatment significantly decreased lipoprotein lipase (lpl) activity in adipose tissue from lean, but not obese, zucker rats and the sa ...19846694985
effect of hypothalamic deafferentation on the distribution of luteinizing hormone-releasing hormone (lhrh) in the rat brain.anterior (ahd) and complete hypothalamic deafferentation (chd) were performed in female rats to ascertain the origin of lhrh detected in the external layer of me. deafferented brains were serially sectioned in a cryostat in the frontal plane. lhrh activity in each section was determined by ria. while ahd, using a knife with a small radius caused no change in the distribution of lhrh in the brain, ahd, using a knife with a large radius resulted in a significant decrease in lhrh content in the arc ...19806777148
para-osteo-arthropathies. a new theory and mode of treatment.the clinical and research experience of para-osteo-arthropathies (poa) observed in paraplegia is presented. from clinical findings and our own research studies, a new theory regarding the etiopathogenesis of the ectopic ossifications has been elaborated. poa is a biological process in which new bone is formed in tissues which in the normal condition do not ossify. a 45ca kinetic study showed that poa are young bones with an active 'turnover'. the urinary hydroxyproline level, already increased f ...19816791273
effects of thyrotropin releasing hormone on hypothalamic thermosensitive neurons of the rat.the actions of thyrotropin releasing hormone (trh) were tested on the firing rat of temperature-sensitive and temperature-insensitive neurons in the rat preoptic/anterior area of the hypothalamus (poa). iontophoretic application of trh resulted in inhibition of the firing rate of temperature-insensitive, warm-sensitive and cold-sensitive neurons. the inhibitory response of cold sensitive neurons in trh was relatively mild, however, and 2 of 6 of these neurons inhibited by trh also displayed rebo ...19816791769
[immunocytochemical characterization of neurosecretory systems producing mesotocin and vasotocin in the brain of several lacertidae].using immunofluorescent techniques vasotocin (vt) and mesotocin (mt) neurosecretory systems were identified in the brain of lacertidae: one european species and of two south-algerian species. in the 3 species mt and vt was shown to be synthetized in specific neurons located in the anterior preoptic area (poa), the supraoptic (son) and in the paraventricular (pvn) nuclei. the axons originating in son and pvn were ending either in the external zone of the rostral median eminence (me) near to the a ...19816800583
differential sensitivity of estrogen target tissues: implications for estrogen regulation of serum luteinizing hormone.in the rat, it is generally accepted that the primary site of estrogen's stimulatory (positive) effects on serum lh is the preoptic area-anterior hypothalamus (poa/ah). in contrast, the primary site of estrogen's inhibitory (negative) effects on serum lh levels has not been conclusively identified. there is evidence to suggest both a medial basal hypothalamic (mbh) and an anterior pituitary site of action. the present studies utilized a unique characteristic of these estrogen effects to investig ...19846708135
cathodal versus anodal stimulation within the preoptic-suprachiasmatic region in male rats: effects on the release of prolactin and luteinizing hormone.the relative efficiency for the release of luteinizing hormone (lh) and prolactin after anodal and cathodal stimulation applied within the preoptic-suprachiasmatic region (poa-sch) was studied in adult male rats under pentobarbital anesthesia. single monopolar pulses 0.5 ms duration, 100 or 200 microa intensity, were applied within the dorsal [vertical (v) 7.3] or ventral (v 7.8 to 7.9) aspects of the poa-sch at a mean rate of 10 hz in 30-s trains every other 30 s for a period of 30 min. blood s ...19836832271
performance of dairy cows in midlactation fed high quality grass pasture and concentrate at three percents of energy requirements.thirty multiparous cows rotationally grazed high quality grass pastures (predominantly dactylis glomerata and poa pratensis) for 140 days. stage of lactation was 54 to 192 days at beginning of trial. cows were offered concentrates to supply 33, 66, or 100% of requirements for net energy for lactation above maintenance; daily intakes by the three groups averaged 3.5, 7.7, and 11.5 kg. refusal of concentrate tended to increase with supplementation. average milk production was 19.2, 20.4, and 21.5 ...19836841749
a regional analysis of estrogen binding to hypothalamic cell nuclei in relation to masculinization and defeminization.gonadal steroids masculinize and defeminize neuroendocrine development, including behavior. defeminization makes males less sensitive than females to estrogen for showing female sexual behavior and cyclic gonadotropin secretion. masculinization makes males more sensitive than females to estrogen for showing male sexual behavior. thus masculinization and defeminization produce opposite effects on estrogen sensitivity. to study the relationship between estrogen sensitivity and estrogen binding, we ...19836842286
characterization of estradiol receptors in brain cytosols from perinatal ferrets.studies were undertaken to characterize the binding of [3h]estradiol ([3h]e2) to blood plasma and to brain cytosols collected from perinatal ferrets of both sexes. a dialysis experiment showed that the binding capacity of plasma for [3h]e2 was low in neonatal ferrets. saturable, high-affinity binding of [3h]e2 to cytosols prepared from ferret hypothalamus + preoptic area (h + poa), basal temporal lobe, and midbrain + brainstem (sexes pooled) was demonstrated 5 days prior to the date of expected ...19836857750
influence of preweaning nutrition on growth of angus calves provided a postweaning nutritional system resulting in discontinuous growth.postweaning growth patterns and preweaning milk and forage intake were observed over a 4-yr period for 137 angus calves allowed either fescue-legume or fescue pastures preweaning. after weaning, calves grazed fescue pasture until spring for a slow growth phase and then fescue, orchard grass, bluegrass and ladino clover pastures for 4 mo. following this period, the calves were confined and fed shelled corn and a 36% crude protein supplement until they reached 12 mm of fat over the 12th rib. the s ...19836874620
role of the preoptic brain in the regulation of preovulatory gonadotropin surges in the hamster.these studies have examined the role of the preoptic-suprachiasmatic area (poa-sc) in brains of cyclic female hamsters in regulating proestrous preovulatory gonadotropin surges. the spontaneous release of lh and fsh which normally occurs on proestrous afternoon was blocked with phenobarbital. temporal changes in serum lh and fsh were measured in such blocked animals after delivery of direct current (100 mua/60 s) to the poa-sc or that portion of the medial basal hypothalamus (mbh) which includes ...19806775974
inhibitory effects of androgens on preoptic area dopaminergic neurons in castrate rats.the present study was undertaken to determine the effects of androgen implants in the preoptic area-suprachiasmatic nucleus (poa-scn) on catecholamine turnover in the poa-scn and the medial basal hypothalamus (mbh) in orchidectomized rats. bilateral implants of testosterone (t) or dihydrotestosterone (dht) in the poa-scn of orchidectomized rats did not affect serum concentrations of lh and fsh or the turnover of dopamine (da) and norepinephrine (ne) in the mbh. in the area surrounding the implan ...19806774276
[pancreatic cancer-related antigen (pcaa) and pancreatic tissue antigen (paa)--their chemical compositions, tissue localization, and clinical significance].pcaa, a glycoprotein antigen identified and purified from ascited fluid of a pancreatic cancer patient and sharing the identical immunogenicity with gelder's poa (shimano, 1981) and paa, a novel pancreas tissue antigen (loor, 1981) purified from saline extract of human pancreas were studied. (1) chemical composition: the purified pcaa from ascites fluid of a pancreatic cancer patient has a molecular weight of 10,000,000. the same antigenic substance as pcaa was also isolated and purified from no ...19836881993
[pancreatic oncofetal antigen (poa)].recently, there has been an increasing interest in the diagnostic and prognostic usefulness of tumor-associated antigen of embryonic and fetal origin. many approaches have been attempted to make an early diagnosis of the pancreatic cancer, where a specific screening blood test for the pancreatic cancer is required. by using antiserum to fetal pancreas, banwo et al, discovered an oncofetal antigen that was present in human fetal pancreas, pancreatic tumor tissue, and sera from patients with the p ...19836882002
[recent progress in tumor markers of the digestive system].recent progress in our tumor marker study of the digestive system was described. of carcinoembryonic antigen (cea), we developed a new assay system for detecting the cea derived from gastrointestinal tumor tissues selectively, and succeeded to diagnose 72% of colorectal cancer patients including dukes' a and b classifications. of mucus antigens, intestinal ima and gastric gma, both were fractionated and purified in our laboratory, were demonstrated to be useful markers in detecting carcinofetal- ...19836876437
changes in estrogen and progestin receptor binding resulting from retrochiasmatic knife cuts.retrochiasmatic frontolateral knife cuts (flc) or sham operations (sham) were performed with a halasz-type knife. all animals were primed with estrogen plus 0.5 mg progesterone (p) and tested for lordosis both before and after surgery. two weeks after the last test they received estradiol (e2) in silastic capsules and were sacrificed 2 days later for determination of either nuclear estrogen receptors or cytosol progestin receptor binding in brain and pituitary (pit). rats which had received flc ...19826890690
sex differences in cholinergic enzymes of diagonal band nuclei in the rat preoptic area.activities of choline acetylase (cat), synthetic enzyme for acetylcholine, and acetylcholine esterase (ache), degradative enzyme for acetylcholine, were measured in preoptic nuclei of gonadectomized, estradiol-treated male and female rats. estradiol-dependent enzymatic changes were localized within discrete poa nuclei and both the location and direction of the changes were dependent on the sex of the rat. estradiol administration increased cat and ache activity in the nucleus of the horizontal l ...19836877524
blood plasma magnesium, potassium, glucose, and immunoreactive insulin changes in cows moved abruptly from barn feeding to early spring pasture.cations and immunoreactive insulin in plasma were measured in 35 lactating cows moved abruptly to early spring pasture. after change of cows from grass-clover hay to fescue-bluegrass pasture containing 22 to 31 g potassium/kg dry matter, immunoreactive insulin of 5 holstein cows increased 30% in 5 days and averaged 45% above prepasture concentrations for 40 days. magnesium averaged 44% below prepasture content of plasma during this period and was correlated negatively with potassium -.17 and imm ...19806999040
effects of pentobarbital on hypothalamic catecholamines and lrh activities.pentobarbital (pnt) injection at 12.30 h to ovariectomized rats suppressed the lrh contents of the medial basal hypothalamus (mbh) in association with a significant fall in serum lh 60-90 min later. the lrh contents in the preoptic area (poa) were unchanged in pnt treated rats. a similar effect on lrh levels in the mbh was also evident in ovariectomized rats pre-treated with oestradiol benzoate. pnt administration rapidly reduced the noradrenergic (ne) turnover in the mbh and poa, whereas dopami ...19807006289
nitrogen fixation (acetylene reduction) by klebsiella pneumoniae in association with 'park' kentucky bluegrass (poa pratensis l.).turks of 'park' kentucky bluegrass reestablished in the greenhouse and inoculated with klebsiella pneumoniae (w6) showed significantly increased nitrogen fixation (acetylene reduction) compared with control turfs. mean ethylene production rates per pot were 368 nmol h-1 for k. pneumoniae treated turfs, 55 nmol h-1 for heat-killed k. pneumoniae treated turfs, and 44 nmol h-1 for untreated turfs. calculated lag periods before activity was observed were generally very short (less than 1 h). when 'p ...19817011516
[effects of hypothalamic deafferentation and estrogen on the distribution and content of hypothalamic lhrh (author's transl)].anterior (ahd), or complete (chd) hypothalamic deafferentation was performed in female rats to ascertain the origin of lhrh in the arcuate median eminence region (arc-me). furthermore, effects of estrogen on the distribution and content of hypothalamic lhrh in ahd castrated rats were examined to elucidate the mechanism of feedback action of estrogen. hypothalamic tissues were sectioned serially at 220 micrometers in thickness in the frontal plane and lhrh activity in each section was measured by ...19817015780
[development of enzyme immunoassay for pancreatic oncofetal antigen (poa) (author's transl)]. 19817024591
inhibition of the postovariectomy depletion of hypothalamic luteinizing hormone releasing hormone (lhrh) by suckling.a study was undertaken to further the understanding of the mechanism by which suckling inhibits the release of pituitary lh and depresses the postovariectomy rise of plasma lh in lactating mammals. to that end, the effect of suckling (10 pups/animal) for 1 or 3 weeks on the lhrh content of the hypothalamus and preoptic area (poa) in ovariectomized and intact rats was examined. controls consisted of intact and 1 or 3 week ovariectomized, nonlactating animals. following decapitation, the brains we ...19827044434
in vivo biosynthesis of hypothalamic luteinizing hormone releasing hormone in individual free-running female rats.the present studies examined the feasibility of performing in vivo biosynthetic studies of hypothalamic lhrh in preoptic area cannulated individual ovariectomized, estradiol benzoate (eb)-progesterone (p) treated rats. one day after eb treatment, rats were bilaterally cannulated in the anterior ventromedial preoptic area (poa). the following day, rats were treated with p, and a mix of 3h-proline and 3h-leucine (500 muci) was continuously infused (for 8 hr) into the poa using an alzet osmotic min ...19827044767
brain grafts reverse hypogonadism of gonadotropin releasing hormone deficiency.hypogonadism in the mutant hpg mouse is characterized by a deficiency of hypothalamic gonadotropin releasing hormone (gnrh). affected male mice exhibit immature reproductive organs, small abdominal testes and low pituitary and plasma gonadotropin concentrations. recent studies have demonstrated the potential of fetal brain transplants to establish functional connections with host tissues. we therefore sought to use this approach to correct the hpg deficit. fetal preoptic area (poa) (a site of gn ...19827045700
effects of discrete lesions of the sexually dimorphic nucleus of the preoptic area or other medial preoptic regions on the sexual behavior of male rats.the sexually dimorphic nucleus of the rat medial preoptic area (sdn-poa) has a volume five times larger in the adult male compared with that of the adult female. in the present study, the effects of discrete electrolytic destruction of the sdn-poa or other specific medial preoptic (mpoa) regions on masculine sexual behavior were determined in adult, sexually experienced male rats. small lesions encompassing the sdn-poa had no effect on the maintenance of copulatory behavior. lesions of similar s ...19836824962
acute thermoregulatory effects of unilateral electrolytic lesions of the medial and lateral preoptic area in rats.unilateral anodal lesions of the medial or lateral preoptic area (poa) in unanesthetized rats had opposite thermoregulatory effects immediately after the lesion were made. lesions of the medial poa evoked hyperthermias and accompanying cold defense responses, including vasoconstriction of the tail, increased oxygen consumption, shivering, and heat conservation postures. the hyperthermias had latencies of 0-30 minutes and reached maximum values within 120 minutes postlesion. they were independent ...19827079312
drug prescribing practices of dentists in the bluegrass dental society area. 19806937590
gastrointestinal tumor markers, other than carcinoembryonic antigen, and alpha fetal protein.oncofetal markers for colon carcinomas are csap, a nonsulfated mucin, a second trimester fetal antigen, an altered thymidine kinase, a monosialoganglioside, and glycolipid antigens. for gastric carcinoma, they are basic fetoprotein, a sulfoglycoprotein, and for pancreatic carcinomas--poa, an oncofetal pancreatic antigen, and designated as capi, an oncofetal antigen. tumor-associated markers for colon carcinomas are: udp-galactosyltransferase and zinc glycinate marker; for gastric carcinomas, sul ...19836883374
changes in dendritic spine density in the preoptic area of the female rat at puberty.preoptic area (poa) neurons stained by a modification of the golgi-kopsch method were studied in developing female rats. particular attention was focused on dendritic spine densities as they might be altered in relation to the onset of puberty. neurons in the anterior superior and posterior superior portions of the poa showed an increase in mean spine density at the age of vaginal opening. the increase in dendritic spine density in the posterior inferior quadrant of poa occurred prior to vaginal ...19827093733
alteration of muscarinic binding in specific brain areas following estrogen treatment.systemic administration of estradiol benzoate (eb) to ovariectomized female rats significantly altered cholinergic muscarinic binding of [3h]quinuclidinyl benzilate in discrete brain regions. specifically, eb increased muscarinic binding in the medial basal hypothalamus (mbh) and decreased muscarinic binding in the medial preoptic area (poa). these alterations were dose-dependent and appeared to reflect changes in the number of muscarinic binding sites. treatment with eb failed to significantly ...19827104717
antidromic identification of neurons in the ventrolateral part of the medulla oblongata with ascending projections to the preoptic and anterior hypothalamic area (poa/aha).seventy neurons in the ventrolateral medulla oblongata were antidromically activated by electrical stimulation of the preoptic and anterior hypothalamic area (poa/aha) in female rats under urethane anesthesia. these identified cells were located within and adjacent to the nucleus reticularis lateralis and could be readily distinguished into at least two types of neurons, designated as "fast' and "slow' cells, on the basis of their waveform and conduction velocity.19827059819
chronic hyperprolactinemia depletes hypothalamic dopamine concentrations in male rats.the tuberoinfundibular dopamine (tida) system appears to tonically inhibit pituitary prolactin secretion while moderate elevations in serum prolactin levels, in turn, augment the turnover rate of dopamine (da) without affecting the steady state concentrations of da in the tida neurons (1-5). the present study demonstrates that chronic elevations in serum prolactin, to greater than 2,000 ng/ml, induced by the prolactin secreting mttw15 tumor, decreased da concentrations by 47% in the median emine ...19827087668
changes in aromatase activity in the rat brain during embryonic, neonatal, and infantile development.we assessed the activity of the aromatase enzyme complex in slices of brain from rats by measuring the release of 3h2o from [1 beta-3h]testosterone. in hypothalami from 12-day-old rats, the rate of aromatase activity was linear with time and amount of tissue. the reaction was saturated at a substrate concentration of 0.1 microm, and the apparent km of the reaction was 27 nm. the production of 3h2o was inhibited by 4-hydroxyandrostenedione, with an apparent ki of 20 nm. aromatase activity was fir ...19827094885
[identification of pancreatic oncofetal antigen (poa) and its clinical significance]. 19827176116
gas-liquid chromatography for evaluating polysaccharide degradation by ruminococcus flavefaciens c94 and bacteroides succinogenes s85.two predominant rumen cellulolytic bacteria, ruminococcus flavefaciens c94 and bacteroides succinogenes s85, were incubated with ground filter paper (whatman no. 1), cattle manure fiber, wheat straw, kentucky bluegrass, alfalfa, and corn silage as substrates. analyses of the initial substrate and the recovered residue after 48 h of static incubation showed that r. flavefaciens c94 was quantitatively more effective than b. succinogenes s85 in degrading total dry matter (32.3% versus 16.1%). howev ...19807189996
forebrain lordosis inhibiting system and serotonin neuron in female rats: effect of p-chloroamphetamine.relationship between forebrain lordosis inhibiting system and serotonergic mechanism was examined in estradiol benzoate (eb) and progesterone (p)-primed ovariectomized rats. in order to remove the forebrain inhibition of lordosis response, anterior roof deafferentation (ard) which interrupts the dorsal inputs to the poa coming down anterior to the anterior commissure or medial preoptic area lesions (poa-l) were made. all experimental females pretreated with eb and p showed high levels of lordoti ...19827151745
differentiation of the sexually dimorphic nucleus in the preoptic area of the rat brain is determined by the perinatal hormone environment.the volume of a sexually dimorphic nucleus in the preoptic area (sdn-poa) of the rat brain is several fold larger in males than in females. when female rats were treated pre- and postnatally with testosterone propionate (tp) their sdn-poa developed equivalent in size to that of normal males. the results indicate that the gross morphological sex difference in sdn-poa volume is exclusively controlled by the hormonal environment during the critical period of sexual brain differentiation.19827162691
neurogenesis of the sexually dimorphic nucleus of the preoptic area in the rat.the sexually dimorphic nucleus of the preoptic area (sdn-poa) of sprague-dawley rats is larger in volume in the male and hormone-dependent early in postnatal life. in the present study, we compared for each sex the time course of neuroblast proliferation which study, we compared for each sex the time course of neuroblast proliferation which forms sdn-poa or adjacent medial preoptic area (mpoa) neurons. additionally, we investigated whether there is a temporal gradient of production of neurons in ...19817217370
effects of histamine implants in several brain regions on the release of prolactin in conscious adult male rats.the levels of prolactin in the plasma of conscious male rats were determined at various times after an acute administration of histamine or a histamine releasing agent, compound 48/80, in three brain regions. the brain structures that were examined were, the caudal part of the preoptic area and anterior part of the anterior hypothalamic area (poa-aha), the arcuate nucleus-ventromedial nucleus region (arc-vmn) and the medial-basal amygdaloid nucleus of the limbic system (ame). a marked increase i ...19817217824
effect of neonatal intrahypothalamic testosterone implants on cyclicity and adult sexual behavior in the female hamster.to localize the neural sites of hormone action for sexual differentiation in the hamster, pellets containing 3 microgram testosterone were implanted directly in the preoptic area-anterior hypothalamus (poa-ah) and the ventromedial hypothalamus-arcuate regions of the hypothalamus of 3-day-old female hamsters. the poa-ah implants strongly enhanced the potential for ectopic mounts, rear mounts, and intromission patterns after adult testosterone treatment. 50% of the females with neonatal poa-ah imp ...19817219677
cyclo (his-pro): mapping hypothalamic sites for its hypothermic action.hypothalamic loci of sprague-dawley rats were individually injected with cyclo (his-pro) to determine the sites where that metabolite of thyrotropin-releasing hormone acts to produce hypothermia. there was almost always a positive hypothermic response in the preoptic-anterior hypothalamic area (poa/aha); injection into the posterior or middle hypothalamic areas or into the hippocampus caused no significant decrease in core temperature. the fact that only injection into the poa/aha evoked hypothe ...19827171986
diagnosis of pancreatic cancer by pancreatic oncofetal antigen (poa) in pure pancreatic juice.pancreatic oncofetal antigen (poa) was detected in the pure pancreatic juice by double immunodiffusion assay in a series of patients, using anti-poa prepared by immunizing rabbits with human fetal pancreas homogenate. the test was positive in as many as 72% of the patients with pancreatic cancer studied, whereas only less than 10% of patients with other diseases or normal controls were positive for this antigen, thus suggesting a potential usefulness of the pure pancreatic juice assay for poa in ...19817216142
cytoplasmic and nuclear binding of estradiol in the brain and pituitary of old female rats.the subcellular distribution of estradiol following the in vivo administration of 3h-estradiol, cytosol and nuclear estrogen receptor after in vitro incubation with 3h-estradiol were studied in young adult female rats (3-6 months old) and old female rats (over 18 months old). after intravenous injection of 3h-estradiol, with or without deithylstilbestrol pretreatment, significantly higher cytosol uptake of estradiol in the pituitary, dorsal hypothalamus and corticomedial amygdala (amyg) with con ...19817215820
[plant antigens with immunological properties. iii. separation of a biologically active fraction in a grass pollen extract].in present paper a partial biochemical study concerning two grass pollens (poa pratensis and phleum pratense) is carried out. on sephadex g-75 three fractions were detected by an extract from the grass pollens. only the third fraction shows a biological activity and biochemical findings are in agreement with the presence of four polipeptidic subfractions of different molecular weights ranged between 10,000 and 14,500. specific additionals trials (ultracentrifugation, disc-electrophoresis, thyn-l ...19807247505
allergenic relationships of two different antigens of kentucky bluegrass pollen.two purified allergens, allergen c and kbg-1, isolated from kentucky bluegrass (kbg) pollen, were found to be antigenically distinct with respect to rabbit precipitating antisera produced separately to each allergen. on the other hand, allergen c and kbg-1 appeared to be allergenically identical in terms of the specificities evaluated with murine reaginic antisera produced to each of these allergens; whereas they appeared to be only partially identical when evaluated with respect to the ige anti ...19817263084
purification and some properties of the main polymorphic form of acid phosphatase from poa pratensis seeds.the main polymorphic form of acid phosphatase was isolated from poa pratensis seeds by chromatography on deae- and cm-cellulose and gel filtration on bio-gel p-100. the enzyme migrated as a single band in disc electrophoresis at ph 4.5 and 8.4. the purified enzyme is a glycoprotein of molecular weight about 33 000. carbohydrate content accounts for 40% of the total weight. the optimum ph is at 5.2 and the apparent km for p-nitrophenylphosphate, 0.55 mm. fluoride ions are non-competitive and zinc ...19807269976
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