Publications

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staggers in sheep in patagonia.after observations and experimental work both in the field and laboratory, the following conclusions seem justified. 1. staggers is a non-infectious disorder affecting horses, cattle, and sheep. 2. the disease is characterized by weakness, muscular twitching, irregular movements of the head, stiffness of the limbs, and transient motor paralysis, accompanied with spastic spasms on excitement. there is also a derangement of vision and conjunctivitis. 3. the postmortem lesions are not characteristi ...191719868185
responses of kentucky bluegrass to variations in temperature, light, cutting, and fertilizing. 193416652876
effect of heavy and late applications of nitrogenous fertilizer on the cold resistance of kentucky bluegrass. 193916653556
chromosome numbers in poa, agropyron, and elymus. 194620280149
hydrocyanic acid poisoning from poa aquatica. 194620262391
stripe smut (ustilago striaeformis) in relation to bluegrass improvement. 194621015647
accessory chromosomes in poa alpina. 194818863984
effects of photoperiod and temperature on growth and flowering of kentucky bluegrass. 194916654204
the relative nutritive value of kentucky bluegrass, timothy, brome grass, orchard grass, and alfalfa. 195015436351
[the relation of bulbil formation in poa alpina vivipara to photoperiodicity and frost]. 195313068387
the standardization of poa annua as an indicator of smog concentrations. i. effects of temperature, photoperiod, and light intensity during growth of the test-plants. 195716655051
comparative effect of ladino clover and bluegrass pasture on fertilization of ova in sheep. 196113746245
stranded arrangement of sporopollenin in the exine of microspores of poa annua.in the early microspore wall of the grass poa annua l., sporopollenin is arranged in bundles of anastomosing strands. each bundle contains at least two strands, each about 50 a in diameter, which anastomose or intertwine and look like a meshwork with pores of the mesh up to 50 a in diameter. after the early microspore stage the exine becomes homogeneous; it is similar in this respect to the exines or ornamented parts of the exines of pollen of other angiosperms examined by electron microscopy.196217749629
opposing actions of light in seed germination of poa pratensis and amaranthus arenicola.action spectra were measured for suppression of germination of poa pratensis l. and amaranthus arenicola i. m. johnston seed under prolonged or continuous irradiation. the action maxima for both types of seeds are near 720 nm. the maxima are unchanged in position or magnitude in the presence of radiation in the region of 600 to 670 nm adequate to maintain phytochrome predominantly in the far-red-absorbing form. a reversible potentiation of germination to change in form of phytochrome was observe ...196816657003
factors influencing agnotobiotic cultures of the rumen ciliate, entodinium simplex.the nutrition of entodinium simplex was studied, with foliage of bluegrass (poa pratense), perennial ryegrass (lolium perenne), and grains of wheat (triticum vulgare) as substrates in agnotobiotic cultures. entodinium grew poorly when the substrates were autoclaved; better growth was obtained when the substrates were sterilized with ethylene oxide vapor. the concentration of ethylene oxide and the amount of moisture influenced the sterility and nutritional adequacy of the treated substrate. auto ...19684969666
seasonal variations in the cyanide content of poa aquatica. 19685245545
transmission of a leafhopper-borne virus from naturally infected poa annua in czechoslovakia. 19685654128
tetanus in the bluegrass area. a report of recorded cases in the hospitals of lexington, kentucky from 1950 to 1968. 19695798168
a diploid population of poa annua l. from australia. 19705483779
influence of acriflavine on the appearance of the factor controlling diplosporia in poa palustris l. 19705527753
chemical control of the "annual bluegrass weevil," hyperodes sp. nr. anthracinus. 19715558279
limited protein and energy for wintering yearling steers on standing kentucky bluegrass. 19715122308
pectolytic and cellulolytic enzymes associated with helminthosporium leaf spot on kentucky bluegrass. 19724672253
the requirement for sodium as a micronutrient by species having the c(4) dicarboxylic photosynthetic pathway.six species having characteristics of plants with the c(4) dicarboxylic photosynthetic pathway, echinochloa utilis l. ohwi et yabuno (japanese millet), cynodon dactylon l. (bermuda grass), kyllinga brevifolia rottb., amaranthus tricolor l. cv. early splendour, kochia childsii hort., and portulaca grandiflora hook (rose moss), responded decisively to 0.1 milliequivalent per liter nacl supplied to their culture solutions initially containing less than 0.08 microequivalent per liter na. chlorosis a ...197216658050
pathogenic potential of tylenchorhynchus dubius on selected turfgrass.tylenchorhynchus dubius was observed to feed on 'toronto' creeping bentgrass and 'merion' kentucky bluegrass, and was a key participant in reducing the vegetative growth of both grass species. the severity of foliar and root macrosymptoms incited by t. dubius to 'toronto' bentgrass was greater on plants grown at 16 c than on plants maintained at 21, 27 and 32 c. these parasitized plants exhibited a suppression of secondary stolon formation, shortened internodes and premature inflorescence initia ...197219319280
effect of tylenchorhynchus nudus on growth of kentucky bluegrass.the effect of tylenchorhynchus nudus on growth of kentucky bluegrass was investigated under controlled environmental conditions in both a phytotron and a greenhouse. the nematode significantly reduced weights of clippings, crowns and roots. pathogenicity was greater in sandy loam soil than in loam and was enhanced by submitting plants to nutrient and/or moisture stresses; soil nutrient level was most critical. the results suggest that t. nudus contributes significantly to summer decline of blueg ...197319319349
[poa (problem oriented approach)-system]. 19734795266
current facts of para-osteo-arthropathy (poa). 19734576824
[on scintigraphy of paraosseous arthropathie (poa) in the hip-joint (author's transl)]. 19744277245
demonstration of nuclear 3h-estradiol binding in hypothalamus and amygdala of female, androgenized-female, and male rats.the subcellular distribution of radioactivity in female, androgenized female, and male rats 1 hour after the iv administration of tritiated estradiol was examined. high regional specificity among nucle ar fractions of brain areas was seen with the preoptic-anterior hypothal amic area (poa-ah), median eminence-basal hypothalamus (me-bh), and amygdala having much higher nuclear binding than other brain areas. nuc lear binding was receptor-mediated in poa-ah, me-bh, amygdala, dorsal hypothalamus ...19744375259
rast, skin and nasal tests and the history in grass pollen allergy.a comparison of the rast (phadebas), skin prick test, nasal test and a history of hay fever was made in 141 subjects, eighty-seven being prick test positive, with three concentrations of extracts of sweet vernal (anthoxanthum odoratum), cocksfoot (dactylis glomerata), meadow fescue (festuca elatior), rye (lolium perenne), timothy (phleum pratense), meadow (poa pratensis). in adequate concentration any of the species extracts would have sufficed for skin tests, with cocksfoot pollen the most pote ...19751053442
3h-estradiol distribution in female, androgenized female, and male rats at 100 and 200 days of age.tissue distribution of radioactivity was studied 48 h after gonadectomy and 1 h after iv injection of 3h-estradiol (1 mug/kg body wt) in 100- and 200-day-old normal female rats, female rats androgenized with 30, 100 or 1250 mug testosterone propionate (tp) at 5 days of age, and male rats. receptor-mediated uptake of estradiol, as shown by diethylstilbestrol (des) competition, was highest in preoptic area-anterior hypothalamus (poa-ah) and median eminence-basal hypothalamus (me-bh), but also occu ...1975163737
localization of luteinizing hormone-releasing hormone in the preoptic area and hypothalamus of the rat using radioimmunoassay.to determine the localization of luteinizing hormone-releasing hormone (lhrh), five brains from adult male rats were serially sectioned in a cryostat at - 10 c in either the frontal, horizontal or sagittal planes. acetic acid-ethanol extracts of each section were assayed for lhrh using radioimmunoassay (ria) and in some cases using bioassay as well. approximately 0.2 ng lhrh was concentrated in medial basal preoptic (mb-po) tissue overlying the rostral portion of the optic chiasm. this lhrh appe ...19751095362
metabolism of siduron in kentucky bluegrass (poa pratensis l.). 19751133302
survival of ancylostoma caninum on bluegrass pasture.the survival of infective larvae of ancylostoma caninum on outdoor grass plots was studied in 40 experiments over 1 year. weather data were collected over the period. mean larval survival from august to early november was 24 days (range 1 to 49), from december through february was 0 days, and from march to mid-august was 6.6 days (range 0 to 21). moderate to high temperatures and substantial rainfall favored larval survival; low temperatures and rainfall favored larval destruction.19751138040
prolactin release induced by electrical stimulation of the hypothalamic preoptic area in unanesthetized sheep.the hypothalamic preoptic area (poa) was electrically stimulated through permanently implanted electrodes in 4 unanesthetized sheep. initiation of poa stimulation was followed witin 10-20 min by large increases in plasma levels of prolactin (prl). the stimulation-induced discharges of prl were consistently observed in both untreated and estradiol-treated castrated animals using 0.1 to 0.7 ma of applied current. since behavioral modifications were only sometimes associated with electrical stimula ...19751143630
effects of hyperthermia and stimulation of the hypothalamus on the activity of the phrenic nerve in hypo- normo- and hypercapnic rabbits.experiments were carried out on male rabbits, anesthetized with urethane, bilaterally vagotomized, paralysed and artificially ventilated. the preoptic area (poa) was stimulated at three different levels of co2: in hypo- normo- and hypercapnia under normothermia, moderate hyperthermia and hyperthermia. changes in central respiratory activity (monitored by phrenic nerve activity) due to co2 levels, stimulation of the poa during normothermia and progressively increased body temperature were investi ...19751163305
female lordosis pattern in the male rat induced by estrogen and progesterone: effect of interruption of the dorsal inputs to the preoptic area and hypothalamus.lordosis behavior was very rare in castrated male rats which had been pretreated with 50 mug estradiol benzoate (eb) for successive 2 days and 1 mg progesterone (p) 6-8 hr prior to testing on the third day. only one out of 8 rats displayed lordosis in response to mounts by the sexually matured males. however, the occurrence of lordosis behavior was markedly increased in similarly treated castrated males in which the dorsal afferents to the preoptic area (poa) and hypothalamus were removed by the ...19751175525
diagnostic value of total ige and antigen specific ige using rast in pollenosis.41 patients with clinically demonstrated pollinosis are studied. the following test were performed on all of them: a) intradermal skin tests using grass pollen extracts (pangramin iii) and with extracts of dactylis glomerate, lollium perenne and poa pratensis. b) totol serum ige determination. c) antigen specific ige (rast) determination against the pollens mentioned in b). d) passive hemagglutination against the pollen dactylis glomerate. basing on the results obtained, we have arrived to the f ...19751180203
thermoregulation in the rabbit following intracranial injection of norepinephrine.the release of norepinephrine (ne) from nerve terminals in the anterior hypothalamic/preoptic area (ah/poa) of the rabbit may serve to raise body temperature. to further examine the putative neurotransmitter role of ne, bilateral microinjections of 5 or 10 mug ne were made into or near the ah/poa of 44 conscious rabbits exposed to an ambient temperature of 15 degrees c. microinjections into the ah/pos did not cause fever; they either had no influence on thermoregulation or rapidly induced ear va ...19751211462
blockade of testosterone-induced mounting behavior in the male rat with intracranial application of the aromatization inhibitor, androst-1,4,6,-triene-3,17-dione.propylene glycol (glycol) solutions containing either testosterone (t) or estradiol (e2) were infused directly into the preoptic area (poa) of longterm castrated rats in order to reinstate male copulatory behavior. in addition, castrated males were administered t or e2 in the poa in combination with a steroid that has been shown to block the aromatization of testosterone to estradiol, androst-1,4,6-triene-3,17-dione (atd). the facilitatory action of testosterone on mounting behavior was blocked ...19751204576
intracranial cycloheximide: effect on male mouse sexual behavior and plasma testosterone.cycloheximide (cyclo), an inhibitor of protein synthesis, infused bilaterally into the preoptic area (poa) of intact b6d2f male mice significantly inhibited male sexual behavior when the males were presented with receptive females 12 hr after treatment. the few males that ejaculated appeared to copulate normally. this finding suggests that cyclo acts primarily by inhibiting sexual arousal rather than sexual performance. the inhibition of sexual behavior was not observed when the males were teste ...19761265105
[plant antigens with immunological properties. ii. further studies of the chemical characteristics and immunological properties of a grass pollen extract].this second study carried out on an extract of two grass pollens (poa pratensis and phleum pratense) demonstrated the presence of n-acetilglucosamine and sialic acid in the extract (rfex). moreover the extract has confirmed its characteristics biologic activity i.e. the inhibition of the phenomenon of immune hemolysis with a different technique too, even having the antibodies of the treated animals the complement fixing activity. no appreciable changes of the immunoelectrophoresis pattern in the ...1976973743
luteinizing hormone requirements for ovulation in the pentobarbital-treated proestrous rat.the lh requirements for ovulation in the pentobarbital-blocked proestrous cd rat have been studied by increasing serum gonadotropin levels through electrical stimulation of the brain and subsequently comparing the effects of timed hypophysectomy on ovulation and serum lh concentrations. the arcuate nucleus (arc) or the medial preoptic area (poa) was stimulated unilaterally for 45 min with matched pairs of biphasic rectangular pulses through a coaxial platinum electrode. serum lh was significantl ...1976987901
a rapid and simple micromethod to measure picograms of norepinephrine (ne) and dopamine (da) in small samples of brain tissue and pituitary gland.a rapid and simple procedure to determine dopamine (da) and norepinephrine (ne) in small tissue samples (mug quantities) is described. the radioenzymatic assay sensitivity, defined as twice the blank, is about 0.1 ng for ne and da. drugs known to modify brain catecholamine metabolism were shown to alter differentially da and ne concentration in the medial basal hypothalamus (mbh) of male rats. different areas of the brain and the pituitary gland, namely the parietal cortex, the pre-optic region ...1976993791
the effect of noradrenaline, injected into the hypothalamus, on thermoregulation in the cat.1. noradrenaline (na) was microinjected into the anterior hypothalamic/preoptic area(ah/poa) of unanaesthetized cats held at ambient temperatures of 10, 22 or 35 degrees c. loci in which injection of na caused body temperature changes were also found to be sensitive to the febrile action of pge1. 2. at all ambient temperatures, na caused a dose-dependent fall in body temperature. however the mechanisms by which these temperature changes were brought about varied at different ambient temperatures ...1976994030
regional and subcellular [3h]estradiol localization in selected brain regions and pituitary of female mice: effects of unlabeled estradiol and various anti-hormones.groups of ovariectomized mice were pretreated for 30 min with either vehicle, unlabeled estradiol benzoate or one of the following anti-hormones: cyproterone acetate, ci-628 or cn-69, 725-27. one hour after an i.v. injection of [3h]estradiol the levels of toluence extractable radioactivity retained in the crude nuclear and cytosol fractions of selected brain regions, pituitary and plasma were determined by liquid scintillation spectrometry. the major findings were: (1) in vehicle treated animals ...19761260424
numerical taxonomy of some yellow-pigmented bacteria isolated from plants.phenetic data on over 60 heterotrophic, gram-negative, yellow chromogenic bacteria from plant material were collected and analysed using numerical taxonomic methods. marker strains representing 42 taxa were included in the analyses. at similarity levels of 80% or above, eight distinct clusters were obtained, the first four of which included yellow chromogens. custer i contained isolates from green healthy leaves of agrostis tenuis, festuca rubra, holcus lanata, lolium perenne and poa pratensis, ...19761011010
changes in the nuclear volume of rat hypothalamic neurons in old age.nuclear volumes (nv) of neurons in the preoptic are (poa), suprachiasmatic nucleus (sc), paraventricular nucleus (pvn), supraoptic nucleus (so), anterior hypothalamic area (aha), arcuate nucleus (arn), ventromedial nucleus (vmn), dorsomedial nucleus (dmn) and lateral mammillary nucleus (ml) were measured in 3.5- to 5-month-old female and male rats and in old female and male rats over 22 months of age. young adult female rats had larger nv than males in the neurons of all measured areas except fo ...19761018731
temporal effects of fornix transection on brain tryptophan hydroxylase activity and plasma corticosterone levels.the effect of fornix transection on plasma corticosterone and on midbrain (mid), septum/preoptic (s/poa) or hippocampal (hip) tryptophan hydroxylase activity (tha) was studied in adult male rats. a mild stress resulted in higher levels of plasma corticosterone in operated as compared to sham-operated rats 6 and 40 h post-transection; however, at 30 days post-operation no difference was found. the response of tha to fornix transection was region-specific. no significant change in the s/poa region ...19761021715
in vivo nuclear 3h-estradiol binding in brain areas of the rat: reduction by endogenous and exogenous androgens.in vivo specific nuclear binding of [2, 4, 6, 7 (n)-3h]estradiol (3h-e2) as indicated by diethylstilbestrol (des)-blockable radioactivity, was measured 1 h after injection in adult intact male, untreated and testosterone propionate (tp)-pretreated or 5alpha-dihydrotestosterone (dht)-pretreated gonadectomized (gdx) male and female rats. there was no sex difference in specific 3h-e2 binding in brain areas and anterior pituitary (pit) of untreated gdx animals. dht pretreatment had no effect on bind ...19761021716
high density lipoprotein (hdl) polymorphisms in rabbit. i. a comparative study of rabbit and human serum high density lipoprotein.different classes of rabbit serum lipoprotein were prepared by ultracentrifugal flotation at densities 1-006, 1-063 and 1-21 g/ml. agarose gel electrophoresis on rabbit whole serum and the serum fractions with different densities showed that this technique separates the different lipoprotein classes reasonably well. the electrophoretic mobility of the different lipoprotein classes of rabbit serum seems to be similar to that of the human lipoproteins, with the exception of alpha1-lipoprotein whic ...1976180184
the anterior hypothalamus: how it affects gonadotropin secretion in the rhesus monkey.the effects of hypothalamic lesions on spontaneous and estrogen-induced lh release were studied in 17 female rhesus monkeys with regular menstrual cycles. in the cycle before surgery, all of the animals experienced 3- to 10-fold increases in serum lh and elevated (above 3 ng/ml) serum concentrations of progesterone. three to 6 days after the onset of menstruation, lesions were made in the preoptic-anterior hypothalamic area (poa-aha) in 14 monkeys by radiofrequency thermocoagulation (rf) or by t ...1976820548
tissue levels of luteinizing hormone-releasing hormone in the preoptic area and hypothalamus, and serum concentrations of gonadotropins following anterior hypothalamic deafferentation and estrogen treatment of the female rat.female rats were ovariectomized prior to anterior hypothalamic deafferentation (ahd) or sham ahd of the medial basal hypothalamus (mbh). lhrh in the mbh and the preoptic area (poa), and the blood concentrations of lhrh, fsh, and lh were measured following decapitation at the end of 30 days in experiment 1 and 28 days in experiment 2. interruption of anterior neural links of the mbh resulted in a drastic reduction in the amounts of lhrh in the mbh and a significant increment in the poa. the circu ...1976780098
differential effects of brain lesions on thirst induced by the administration of angiotensin-ii to the preoptic region, subfornical organ and anterior third ventricle.(1) the possibility that water intake elicited by the administration of angiotensin-ii to the preoptic region (poa), subfornical organ and anterior third ventricle is mediated by separate neural systems was investigated in 58 male wistar rats using electrolytic lesion techniques. (2) lesions of the midlateral hypothalamus and paramedial rostral midbrain produced a significant reduction in water intake to angiotensin-ii microinjected into the poa but did not affect drinking following administrati ...19761276900
cyanide poisoning of cattle grazing "reed sweet-grass"abstract extract reed sweet-grass, poa aquatica (glyceria maxima, glyceria acquatica), a member of the gramineae family, is an introduced plant found in wet places in various localities in both the north and south islands, but mainly in otago and southland (allan, 1940 ). garner ( 1961 , p. 318) quotes quisumbine (1947) as listing 25 species belonging to the family gramineae, including poa aquatica, which contain hydrocyanic acid. connor (1951) , however, does not list poa aquatica as on ...197622133056
sensitivity of anterior hypothalamic areas to gonadal steroid implantation in androgenized female rats.a single injection of 1 mg of a complex of testosterone esters on day 5 of life was used to prepare constantly oestrous rats. such androgenized female rats were then ovariectomized and submitted to stereotaxical implantation of 1 microgram oestradiol benzoate, 5 microgram testosterone isobutyrate or, as a control, 10 microgram cholesterol in the anterior hypothalamic areas. the effects of the steroids on plasma and pituitary fsh and lh were assessed by radioimmunoassay. as reported previously by ...1977561152
quantitative assessment of para-osteo-arthropathy and its maturation on serial radionuclide bone images.paraplegics whose range of motion is limited by para-osteo-arthropathy (poa) may have difficulty in becoming independent unless the heterotopic bone mass is removed. the recurrence rate is high if the bony mass is not mature at the time of surgery. radiography and alkaline phosphatase correlations are not trustworthy. in 3 paraplegics with poa, radiolabeled osteotropic agents demonstrated a steady decrease in the uptake ratio (heterotopic/normal bone) followed by a steady-state plateau, reflecti ...1977403570
new technique of injection of estradiol into preoptic-anterior hypothalamic area of newborn rats: technique limiting diffusion and duration, with preliminary results on postpuberal block of ovulation.previous studies have shown that sc administration of androgen or estrogen into newborn female rats will disturb postpuberal ovulatory function, as evidenced by a higher incidence of vaginal estrus, few ovarian corpora lutea, and the abolition of estrogen-induced luteinizing hormone surges after ovariectomy and chronic estrogen maintenance. studies of the uptake of labeled estrogen and intracerebral lesion studies have indicated the preoptic area and anterior hypothalamic nuclei (poa-ah) as poss ...1977401032
studies on hypothalamic estradiol binding during estrous cycle and after ovariectomy.specific binding of 3h-estradiol (1 microgram kg-1 injected s.c. 1 h before sacrifice) in the hypothalamus, anterior pituitary and uterus of cycling and ovariectomized female rats was investigated. preoptic-anterior hypothalamic area (poa-ah) and median eminence-basal hypothalamus (me-bh) were separated by gross dissection from the bulk of hypothalamus. pieces of parietal cortex served as controls. the lowest amount of 3h-estradiol specifically bound to cytoplasmic and nuclear receptors in poa-a ...1977305342
identification of aromatase in the reptilian brain.the present study tests the hypothesis that brain aromatase is an "ancient" property of nervous tissue and may be identified in homologues of the limbic system in a non-mammalian vertebrate, the turtle chrysemys picta. tissue homogenates (180 mg wet weight/2 ml) were incubated with [7 alpha-3h]androstenedione and cofactors for 60 min at 37 c. estrone (e1) was isolated and characterized by thin layer chromatography, methylation and recrystallization to constant specific activity. no estradiol-17 ...1977837883
[relationship between the skin test and the specific ige (radio-allergo-sorbens test) in the diagnosis of inhalative allergies].immediate-type reactions such as asthma, pollinosis and several forms of urticaria can be regarded as type i reactions according to coombs and gell. primary these diseases are caused by the immune globulin e. as skin testing is accompanied by numerous failors, there is a real possibility in increasing the diagnostic relevance by measurement of the specific ige in serum (radio-allergo-sorbens-test = rast; phadebas-rast -- pharmacia uppsala). there are often found divergent results and therefore t ...1977842065
hypothalamic areas involved in prostaglandin (pg)-induced gonadotropin release. i: effects of pge2 and pgf2alpha implants on luteinizing hormone release.ovariectomized rats had a cannula inserted unilaterally within various hypothalamic areas. several days later they were primed with a sc dose of 10 microng of estradiol benzoate (eb). two days after priming they were etherized and an initial blood sample was drawn from the external jugular vein. an inner cannula containing pge2 or pgf2alpha at its tip was inserted into the previously implanted outer cannula. blood samples were drawn at 20, 40, 60, and 120 min following the implantation. pge2 ind ...1977870307
effects of hypothalamic and preoptic lesions on reproductive behavior in male rats.adult male rats, which were selected on the basis of showing complete patterns of male copulatory behavior on two prior tests, were castrated six weeks prior to brain surgery. animals were divided into three groups and given small bilateral lesions in the dorsomedial preoptic area (poa), ventromedial hypothalamus (vmh), or sham operations. starting 10 days postsurgery, all animals were injected with estrogen alone and estrogen plus progesterone, and tested twice for lordosis behavior. ten days f ...1977880486
effect of prostaglandin, pyrogen and noradrenaline, injected into the hypothalamus, on thermoregulation in newborn lambs.thirteen newborn lambs were implanted stereotaxically with an array of 4 guide tubes positioned bilaterally so that their tips lay above various hypothalamic loci. at postnatal ages 50-70 h, rectal temperature was monitored, and bilateral 1 microliter injections were made into the hypothalamus. prostaglandin e1 (0.2 microgram) and prostaglandin e2 (0.2-2.0 microgram) did not cause fever on any occasion though injections were made into 44 sites throughout the hypothalamus. nevertheless, lambs tha ...1977884495
hypothalamic areas involved in prostaglandin (pg)-induced gonadotropin release. ii: effect of pge2 and pgf2alpha implants on follicle stimulating hormone release.the effect of unilateral intrahypothalamic implants of pge2 or pgf2alpha on fsh release in ovariectomized, estrogen-treated rats was studied. both pgs induced an equally small increase in plasma fsh following their implantation in the body of the arcuate nucleus-median eminence region (barh-me). levels were maximally elevated 120 min post-implantation. pge2 implanted in the caudal portion of the arh-me (tarh-post-me) also induced a similar increase in plasma fsh, whereas its placement in the reg ...1977870308
electrophysiological and pharmacological studies of amygdalo-hypothalamic connections in the rat.electrophysiological techniques have been used to investigate the neural connections between the amygdala and the rostral hypothalamus of female rats. recordings have been made from 120 neurones in the preoptic/anterior hypothalamic area (poa/aha) which could be driven by electrical stimulation of the amygdala. 89% of the neurones were activated orthodromically and, of these, 2 groups have been distinguished according to the latency of the responses. the remainder of identified cells were antidr ...1977909625
circadian periodicities of serum androgens, progesterone, gonadotropins and luteinizing hormone-releasing hormone in male rats: the effects of hypothalamic deafferentation, castration and adrenalectomy.serum testosterone (t), dihydrotestosterone (dht), progesterone (p), lh, fsh and luteinizing hormone-releasing hormone (lhrh) levels and the lhrh content in the medial basal hypothalamus (mbh) and preoptic area (poa) were estimated during a 24-h period. circadian rhythms, temporally unrelated to each other, were evident in serum fsh and lhrh and the mbh lhrh content; serum lh fluctuated randomly. in addition, serum t and dht demonstrated a parallel circadian pattern while serum t and p appeared ...1977338291
a daily neural signal for luteinizing hormone release in the untreated ovariectomized rat: changes in gonadotropin-releasing hormone content of the preoptic area and hypothalamus throughout the day.gonadotropin-releasing hormone (gnrh) content of preoptic areas (poa) and hypothalami, and serum gonadotropins of rats ovariectomized six weeks earlier were measured throughout the day in two experiments. in the first, rats were decapitated at 2 h intervals between 0800 and 1800 h. the entire preoptic-hypothalamic region was removed and extracted for radioimmunoassay (ria) of gnrh. serum gonadotropins measured by ria were highly variable but mean concentrations were not significantly different t ...1977322997
differential response of luteinizing hormone-releasing hormone in the basal hypothalamus and the preoptic area following anterior hypothalamic deafferentation and/or castration in male rats.serum lh, fsh and lhrh concentrations and the lhrh content in the medial basal hypothalamus (mbh) and the preoptic area (poa) were measured by radioimmunoassay in male rats 33 days after anterior hypothalamic deafferentation (ahd) and/or castration. in castrate rats following ahd, there was a significant decrease in serum lh, fsh, and lhrh concentrations, whereas, in intact rats, serum lh was elevated in ahd over the sham ahd rats. castration and ahd each caused a significant fall in the lhrh le ...1977318623
purification and partial characterization of the apoa-i of rabbit high density lipoprotein.purification of apoa-i from rabbit high density lipoproteins (hdl) gave one single band in sodium dodecylsulphate-polyacrylamide disc electrophoresis and reacted only with antiserum to apoa-i. the molecular weight was about 25000. the amino acid composition of rabbit ,poa-i gave a difference index of 7.4 compared to human apoa-i and of 6.5 compared to dog apoa-i. of the three carboxy terminal amino acid residues the rabbit protein has one in common with the dog. the amino acid sequence of twenty ...1978205614
luteinizing hormone secretion in hemidecorticate female rats under several experimental conditions.luteinizing hormone (lh) secretion by the anterior pituitary gland was investigated in hemidecorticate (hd) rats under several conditions. higher plasma and lower pituitary lh levels were observed in hd rats in the afternoon of proestrus, after unilateral ovariectomy, and at the 7th and 14th days after bilateral castration. the ovarian hypertrophy observed in hd rats did not differ from control. when hormonal substitutive therapy was started 3 days after bilateral ovariectomy, larger doses of es ...1978567287
responses and pharmacological properties of preoptic/anterior hypothalamic neurones following medial forebrain bundle stimulation.1. the responses of neurones in the anterior hypothalamic and preoptic areas (poa/aha) to stimulation of the medial forebrain bundle (mfb) have been studied in urethane anaesthetized female rats. extracellular unit recordings have been made from 150 neurones which were responsive to a single stimulus applied to the mfb at the level of the mammillary nucleus. 2. forty-five per cent of these cells were orthodromically activated with latencies ranging from 7.5 to 100 msec. however, the majority of ...1978307601
cardiovascular changes in anaesthetised rats after the intra-hypothalamic administration of adrenaline.bilateral injections of adrenaline (0.01-10.0 microgram) into the anterior hypothalamic (ah) region, in urethane-anaesthetised spontaneous hypertensive (sh) rats, elicited dose-dependent falls in blood pressure and heart rate. the bradycardia was immediately in onset while the hypotension was preceded by a short-lasting rise in blood pressure. bilateral injections of adrenaline into the anterior preoptic area (poa) and areas surrounding the ah had little or no effect on blood pressure and heart ...1978755652
autoradiographic localization of androgen-concentrating cells in the brain of the male domestic fowl.cells in the male fowl brain which accumulate radioactivity following 3h testosterone (t) administration were identified by autoradiography. labelled cells were found principally in hypothalamic, limbic and midbrain structures. marked uptake was observed in the preoptic area (poa) and in the anterior and posterior hypothalamus. there was also a significant amount of labelling in the archistriatum (arch), particularly in the nucleus taeniae (tn), and in the lateral septum. in the midbrain, substa ...1978683476
independent masculinization of neuroendocrine systems by intracerebral implants of testosterone or estradiol in the neonatal female rat.small pellets of testosterone (t) or estradiol (e2), but not cholesterol (ch), when implanted into the brain of neonatal female rats on day 2 or day 5 of life, produce masculinization of the adult regulation of gonadotropic hormone (gth) release, female sexual behavior or masculine sexual behavior, specific to the site and time of implantation and the hormone implanted. site specificity: there appear to be specific neuronal sites where implantation of t or e2 produces independent masculinization ...1978647393
preoptic-hypothalamic control of lh and prolactin release in male rats under pentobarbital anesthesia: differential responses to electrical stimulation.in acute experiments under pentobarbital anesthesia, the brains of adult male rats were stimulated bilaterally with trains of monophasic pulses at different frequencies and current intensities through bipolar, concentric, stainless steel electrodes. stimulation of the poa-sq region at 3, 10 (200 mua) or 200 hz (100 mua) increased plasma prolactin levels within 30 min of the onset of stimulation. stimulus parameters fully effective when applied to the poa-sq or arc-med. em. regions were ineffecti ...1978638765
stereotyped behavior and hyperthermia in dogs: correlation with the levels of amphetamine and p-hydroxyamphetamine in plasma and csf.a gas-chromatographic method for simultaneously measuring p-hydroxyamphetamine (poa) against amphetamine (a) in plasma and csf is presented. the time course of body temperature (tb), stereotyped behavior (st), and a and poa levels in plasma and csf were studied after administration of 0.6 and 1.5 mg/kg p.o. of a to dogs. stereotyped behavior reached maximal value 2.5 h after a, as did levels of a in csf. the a levels in csf decreased steadily in the following hours and simultaneously with the le ...197897724
studies on an oncofetal antigen, poa.this paper describes the purification and partial characteristics of a putative oncofetal antigen, poa, which appears to be associated with the pancreas. poa is a glycoprotein of molecular weight between 800,000 and 900,000 daltons. it is found in fetal pancreas and pancreas cancer tissue, but not in normal adult pancreas. it is clearly different from carcinoembryonic antigen, other known tumor associated antigens, acute phase reactants and normal serum proteins. a quantitative rocket immunoelec ...1978101295
effects of nematicides on nematode densities in turf in connecticut.the plant-parasitic nematodes criconemoides lobatum, hoplolaimus tylenchiformis, and tylenchorhynchus dubius were present in the top 7.5 cm of sod consisting of numerous stolons and fibrous roots. phenamiphos and 1,2-dibromo-3-chloropropane (dbcp) controlled all three species, whereas ethoprop and oxamyl controlled h. tylenchiformis and t. dubius but not c. lobatum. benomyl and carbofuran controlled h. tylenchiformis but had poor control of c. lobatum and t. dubius. the effectiveness of carbofur ...197819305826
further studies on the effects of lesions in the rostral hypothalamus on gonadotropin secretion in the female rhesus monkey (macaca mulatta).bilateral radiofrequency lesions were stereotaxically placed in the rostral hypothalamus of four adult female rhesus monkeys. these lesions resulted in extensive destruction of the ventromedial preoptic-anterior hypothalamic area (poa-aha) and included the suprachiasmatic nucleus as well as, with the exception of one animal, the organum vasculosum of the lamina terminalis. in three of these four animals, gonadotropin surges similar to those observed before surgery were elicited in response to ei ...1979110581
paradoxical effect of amphetamine in an endogenous model of the hyperkinetic syndrome in a hybrid dog: correlation with amphetamine and p-hydroxyamphetamine blood levels.a telomian-beagle hybrid has been studied as a possible model for the hyperkinetic syndrome in children. behavior tests showed that hybrids, like children, exhibit hyperactivity, impulsiveness, and impaired learning. two groups of hybrid could be differentiated; the behaviour of one improved after amphetamine (responders) while that of the other did not (nonresponders). moreover hybrids were less responsive than beagles to other effects of amphetamine such as stereotyped behaviour and hypertherm ...1979111287
activation of feminine sexual behavior in castrated male rats by intrahypothalamic implants of estradiol benzoate.the effects of intracerebral estradiol benzoate (eb) implants upon lordosis behavior in castrated male rats were investigated. 27 guage eb-filled or blank implants were placed unilaterally in either the preoptic area (poa) or the region of the ventromedial hypothalamic nucleus (vmh). when 0.5 microgram of eb was administered subcutaneously for eight days, males with eb implants had significantly higher lordosis quotients (lq) than males with blank implants. eb implants located in the region of t ...1979571538
nitrogen fixation associated with 'park' kentucky bluegrass (poa pratensis l.).associative nitrogen fixation in kentucky bluegrass (poa pratensis l.) turfs inoculated with five nitrogen-fixing bacterial isolates was evaluated using the acetylene reduction assay and nitrogen accumulation as indicators of fixation. 'park' and 'nugget' kentucky bluegrass turfs were grown in controlled environment chambers and inoculated with klebsiella pneumoniae (w-2, w-6, and w-14), erwinia herbicola (w-8), and enterobacter cloacae (w-11). 'park' inoculated with k. pneumoniae (w-6) had sign ...1979394819
dynamic changes in hypothalamic lh-rh levels associated with the ovarian steroid-induced gonadotrophin surge.pro-oestrous-type lh surges were induced by treating ovariectomized rats (day 0) with either oestradiol benzoate (oeb, 40 micrograms/rat) or oestrogen (10 micrograms/rat followed by progesterone (4 mg/rat, day 2, oebp). to characterize the temporal changes associated with the lh surge on day 2, lh-rh levels in the medial basal hypothalamus (mbh) and the pre-optic area (poa) were analyzed by ria in rats sacrified at hourly intervals between 10.00--19.00 h. following these treatments the lh-rh con ...1979386683
fever produced by intrahypothalamic pyrogen: effect of protein synthesis inhibition by anisomycin.in the unrestrained cat, the inhibition of protein synthesis by anisomycin, given either subcutaneously (5.0--25.0 mg/kg) or directly into the anterior hypothalamic, preoptic area (1.0--25.0 micrograms) impaired the development of a bacterial fever. s. typhosa infused intravenously (1:10 dilution in 1 ml) or into ah/poa (1.0 microliter) evoked an intense fever which was either significantly delayed or prevented by anisomycin. conversely, anisomycin failed to affect the typical hyperthermia evoke ...1979526856
hypothalamic injection of cycloheximide in the 4-day cyclic rat. acute suppression of lordotic behavior and ovulation.the results of serveral studies imply that estrogen can act upon the central nervous system via a protein synthetic step. our objective was to determine if the intrahypothalamic (preoptic area, poa) injection of cycloheximide (chx), an inhibitor of protein synthesis, at 17.00 h on diestrus ii of the 4-day cycle altered lordotic behavior and (or) ovulation in the intact rat (sexual receptivity to males normally begins on the evening of proestrus as herein defined; ovulation occurs on estrus of th ...1979514453
influence of midbrain stimulation on the excitability of neurons in the medial hypothalamus of the rat.extracellular action potentials were recorded from 1098 neurons in the medial hypothalamus of pentobarbital anesthetized male rats. their excitability was analyzed after single 1 hz stimulation of the midbrain periaqueductal gray (pag) or adjacent reticular formation. cells were also examined for their response to median eminence (me), amygdala, lateral septum (ls) or anterior hypothalamic/preoptic area (aha/poa) stimulation. antidromic invasion from midbrain stimulation was recorded from 110 ne ...1979487122
direct septo-hypothalamic projections in the rat.axonal projections from neurons located in the medial and lateral septal nuclei (msn and lsn) were traced autoradiographically. the msn projected bilaterally via a midline route to the diagonal band of broca (dbb), the preoptic area (poa), the suprachiasmatic (scn), paraventricular (pvn), ventromedial (vhm) and arcuate (arc) nuclei including the median eminence (fiberous zone). lateral coursing fibers traveled with the medial forebrain bundle (mfb) to the supraoptic (son) and pre- and supra-mamm ...1979313542
blockade of progesterone-induced increase in hypothalamic luteinizing hormone-releasing hormone levels and serum gonadotropins by intrahypothalamic implantation of 6-hydroxydopamine.the present study was undertaken to identify the hypothalamic locus where norepinephrine (ne) nerve terminals communicate with luteinizing hormone-releasing hormone (lh-rh)-containing neurons involved in the stimulatory feedback action of gonadal steroids on lh and fsh release. ovariectomized rats received estradiol benzoate (10 microgram/rat s.c.) on day 0. intracranial implants containing either 6-hydroxydopamine (6-ohda), to destroy ne terminals, or cocoa butter (controls) were placed bilater ...1979223726
involvement of catecholaminergic nerve fibers in angiotensin ii-induced drinking in the japanese quail, coturnix coturnix japonica.monamine distribution in a septohypothalamic area was investigated in the japanese quail using a histochemical fluorescence method. this area includes the subfornical organ (sfo) and the preoptic area (poa) which are inferred dipsogenic receptor sites for angiotensin ii (aii) in the japanese quail. nerve fibers showing yellow-green fluorescence were found between the poa and the sfo. thwy traversed from the poa to the sfo, and some fibers seemed to terminate on the neurons in the sfo. after a lo ...1979487128
effect of hypothalamic deafferentation on the distribution of luteinizing hormone-releasing hormone (lhrh) in the rat brain.anterior (ahd) and complete hypothalamic deafferentation (chd) were performed in female rats to ascertain the origin of lhrh detected in the external layer of me. deafferented brains were serially sectioned in a cryostat in the frontal plane. lhrh activity in each section was determined by ria. while ahd, using a knife with a small radius caused no change in the distribution of lhrh in the brain, ahd, using a knife with a large radius resulted in a significant decrease in lhrh content in the arc ...19806777148
gas-liquid chromatography for evaluating polysaccharide degradation by ruminococcus flavefaciens c94 and bacteroides succinogenes s85.two predominant rumen cellulolytic bacteria, ruminococcus flavefaciens c94 and bacteroides succinogenes s85, were incubated with ground filter paper (whatman no. 1), cattle manure fiber, wheat straw, kentucky bluegrass, alfalfa, and corn silage as substrates. analyses of the initial substrate and the recovered residue after 48 h of static incubation showed that r. flavefaciens c94 was quantitatively more effective than b. succinogenes s85 in degrading total dry matter (32.3% versus 16.1%). howev ...19807189996
drug prescribing practices of dentists in the bluegrass dental society area. 19806937590
blood plasma magnesium, potassium, glucose, and immunoreactive insulin changes in cows moved abruptly from barn feeding to early spring pasture.cations and immunoreactive insulin in plasma were measured in 35 lactating cows moved abruptly to early spring pasture. after change of cows from grass-clover hay to fescue-bluegrass pasture containing 22 to 31 g potassium/kg dry matter, immunoreactive insulin of 5 holstein cows increased 30% in 5 days and averaged 45% above prepasture concentrations for 40 days. magnesium averaged 44% below prepasture content of plasma during this period and was correlated negatively with potassium -.17 and imm ...19806999040
effects of pentobarbital on hypothalamic catecholamines and lrh activities.pentobarbital (pnt) injection at 12.30 h to ovariectomized rats suppressed the lrh contents of the medial basal hypothalamus (mbh) in association with a significant fall in serum lh 60-90 min later. the lrh contents in the preoptic area (poa) were unchanged in pnt treated rats. a similar effect on lrh levels in the mbh was also evident in ovariectomized rats pre-treated with oestradiol benzoate. pnt administration rapidly reduced the noradrenergic (ne) turnover in the mbh and poa, whereas dopami ...19807006289
role of the preoptic brain in the regulation of preovulatory gonadotropin surges in the hamster.these studies have examined the role of the preoptic-suprachiasmatic area (poa-sc) in brains of cyclic female hamsters in regulating proestrous preovulatory gonadotropin surges. the spontaneous release of lh and fsh which normally occurs on proestrous afternoon was blocked with phenobarbital. temporal changes in serum lh and fsh were measured in such blocked animals after delivery of direct current (100 mua/60 s) to the poa-sc or that portion of the medial basal hypothalamus (mbh) which includes ...19806775974
converging visual and somatic sensory cortical input to the intraparietal sulcus of the rhesus monkey.a cyto- and myeloarchitectonic study reveals the presence of a distinct cortical zone ("area poa") in the lower bank of the intraparietal sulcus of the rhesus monkey. using both autoradiographic and silver impregnation techniques, an analysis of cortical connections shows two overlapping projections to this sulcal zone. these come from (1) the middle portion of the preoccipital gyrus (area oa) and (2) the rostral inferior parietal lobule (area pf).19806769545
inhibitory effects of androgens on preoptic area dopaminergic neurons in castrate rats.the present study was undertaken to determine the effects of androgen implants in the preoptic area-suprachiasmatic nucleus (poa-scn) on catecholamine turnover in the poa-scn and the medial basal hypothalamus (mbh) in orchidectomized rats. bilateral implants of testosterone (t) or dihydrotestosterone (dht) in the poa-scn of orchidectomized rats did not affect serum concentrations of lh and fsh or the turnover of dopamine (da) and norepinephrine (ne) in the mbh. in the area surrounding the implan ...19806774276
[plant antigens with immunological properties. iii. separation of a biologically active fraction in a grass pollen extract].in present paper a partial biochemical study concerning two grass pollens (poa pratensis and phleum pratense) is carried out. on sephadex g-75 three fractions were detected by an extract from the grass pollens. only the third fraction shows a biological activity and biochemical findings are in agreement with the presence of four polipeptidic subfractions of different molecular weights ranged between 10,000 and 14,500. specific additionals trials (ultracentrifugation, disc-electrophoresis, thyn-l ...19807247505
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