Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| fractionation of cocksfoot (dactylis glomerata) pollen by preparative isoelectric focussing. | the technique of isoelectric focussing in polyacrylamide gel has been developed to a preparative mode. using the improved technique cocksfoot (dactylis glomerata) pollen has been split into many components, and the allergenic reactions of the preparations have been studied by passive transfer in animal skin and by rast. | 1978 | 23399 |
| cellular histamine release, specific and total serum ige levels in hay fever patients and controls. | the amount of histamine released from blood leucocytes by allergen, the amount of allergen required to release 50% histamine and the total ige and ige specific for dactylis glomerata are compared in nine hay fever patients and five control individuals. the variation with time of total ige, of specific igee and of the percentage of histamine released at a fixed allergen concentration, corresponding to the cellular sensitivity, is shown for one control individual with positive in vitro and skin te ... | 1978 | 77195 |
| allergenic activity of fractions of cocksfoot (dacylis glomerata) pollen. | a cocksfoot (dactylis glomerata) pollen extract has been fractionated by isoelectric focusing in polyacrylamide gel, the separated components extracted from the gel and assayed by three methods: prick tests in human skin, rast and pca tests in monkey skin. prick testing in human skin showed that subjects responded differently to the separated components, indicating the presence of more than one antigenic determinant, and a profile of antibody activity against each fraction could be constructed. ... | 1978 | 414856 |
| an anomalous effect of allergen concentration on weal size elicited by skin testing for immediate hypersensitivity. | we report the finding of anomalous responses in the skin of atopic individuals when prick tested with serial dilutions of cocksfoot grass pollen extracts. each pollen extract was standardized using rast inhibition activity related to the who standard for ige. 75% of the patients gave a larger weal area with an allergen concentration of 1:10(5) than with the next higher concentration, i.e. 1:10(4). possible mechanisms whereby a larger skin weal is elicited with a lower concentration of allergen a ... | 1979 | 421334 |
| species specific grass pollen sensitivity: diagnosis and treatment with single grass species allpyral vaccines. | skin test titrations and nasal provocation tests in sixty patients with hay fever showed specific reactions to extracts of individual grass species. there was, however, no correlation between skin and nasal sensitivity. repeat testing after treatment with allpyral vaccines consisting of only the grass species to which the nasal reaction was most severe, or only one of several pollens to which reactions were equally severe, showed marked diminution of skin and nasal sensitivity not only to the si ... | 1979 | 498489 |
| variations in the digestibility of trace elements (cu, zn and mn) during the first cycle of cocksfoot growth. | the variations of trace element availability was examined with 6 adult male sheep (texel) maintained in metabolic crates. they received cocksfoot cut each morning during the first vegetation cycle and offered ad libitum (10 % refused food), for 6 day periods. it appears from the resulting data that: -- the stage of cocksfoot development has a strong influence upon the digestibility and the retention of copper, zinc and manganese. --there is an almost perfect parallelism between the variations of ... | 1977 | 606139 |
| enzyme determination and rast inhibition assays for orchard grass (dactylis glomerata): a comparison of commericial pollen extracts. | pollens release enzymes when they are dropped into water. orchard grass extracts were tested by two methods. the api-zym system determined 19 enzymes and colorimetric amounts of leucine aminopeptidases, acid phosphatase and trypsin. these were compared with rast inhibition assays. a good correlation was shown between the techniques. thus the enzyme dosage may be useful for the standardization of pollen extracts. | 1978 | 686510 |
| [decomposition of grass-fructosanes by lactobacilli from silage (author's transl)]. | lactobacilli isolated from grass silage were shown to belong to lb. plantarum, lb. casei and lb. brevis. in studies on the degradation of fructosanes significant differences among the three species were found. while all strains of l. casei were able to degrade fructosanes from dactylis glomerata or phleum pratense regardless of the degree of polymerization, no nember of lb. plantarum or lb. brevis could attack these polysaccharides. | 1976 | 827143 |
| search for a correlation between familial allergy to cocksfoot pollen and hla antigens. | twelve families comprising 62 individuals, 31 allergic to grasses, were examined. no significant correlation between pollinosis and the hla system was observed. however, there was a close correlation between cocksfoot skin tests and the rast (p less than 10(-6)). | 1977 | 876127 |
| demonstration of reaginic antibodies on human basophils by immune adherence to allergen-coated sepharose beads. | human basophils purified by gradient density centrifugation and differential glass bead adherence were interacted with grass pollen allergen in particulate form prepared by covalent coupling of dactylis glomerata protein concentrate to sepharose beads. basophils from allergic subjects were found to interact specifically with the allergen-coated bead surface in a manner so highly characteristic that false negatives with basophils from non-allergic subjects or subjects with unrelated allergies wer ... | 1976 | 963864 |
| [obtaining mutations with cytoplasmic male sterility (cms) in dactylis glomerata l. under the influence of chemical mutagens]. | when treating seeds of orchard grass with definite concentrations of nitrosoethylurea (neu), dimethyl sulphate (dms) and ethylene imine (ei) in m1 and m2 the plants with cms as well as sterility fixators to them are obtained. proportionality of mutagenic action and stimulation effect of these substances are found. the greatest amount of sterility fixators is found in m1 among plants the seeds of which were treated with neu and ei; in m2 the fixators are found among plants obtained after treating ... | 2013 | 1030857 |
| rast, skin and nasal tests and the history in grass pollen allergy. | a comparison of the rast (phadebas), skin prick test, nasal test and a history of hay fever was made in 141 subjects, eighty-seven being prick test positive, with three concentrations of extracts of sweet vernal (anthoxanthum odoratum), cocksfoot (dactylis glomerata), meadow fescue (festuca elatior), rye (lolium perenne), timothy (phleum pratense), meadow (poa pratensis). in adequate concentration any of the species extracts would have sufficed for skin tests, with cocksfoot pollen the most pote ... | 1975 | 1053442 |
| examination of cocksfoot pollen (dactylis glomerata) extracts by skin prick test in man, rast-based allergen assay and protein content. | three commercial preparations of cocksfoot pollen (dactylis glomerata) from different manufacturers were compared by skin prick tests in man, allergen activity as measured by the rast test and protein nitrogen unit (pnu) and total nitrogen (n) content. the skin prick tests were made on the forearm with the undiluted preparations and also with solutions of 1/5 and 1/25 dilutions. three groups each of 9 subjects were tested and the sites for the 9 tests were determined by 3 different latin squares ... | 1975 | 1149937 |
| cutaneous reactions to pollen extracts in patients with hay fever. special references to grass pollen recommended for treatment in fixed combination. | skin reactivity to 8 common grass pollens was investigated in patients with hay fever using commercial, unfractionated extracts in serial dilution technique. the highest degree of sensitivity was observed for cocksfoot, meadow fescue, meadow foxtail, rye grass, timothy and yorkshire fog. five of those are included in the allpyral 5-grass mix. the cutaneous responses to rye grass pollen overlap with those to timothy in a high degree. | 1975 | 1173992 |
| forage systems for beef production from conception to slaughter: ii. stocker systems. | fall weaned angus calves grazed stockpiled 1) tall fescue (festuca arundinacea schreb.), 2) tall fescue-red clover (trifolium pratense l.), or 3) tall fescue-alfalfa (medicago sativa l.) or were barn-fed, 4) tall fescue hay, 5) orchardgrass (dactylis glomerata l.)-alfalfa hay, or 6) tall fescue silage from late october to early april during each of 5 yr. infection of the fescue with acremonium coenophialum ranged from 0 to 55%. there were two replications each of steers and heifers for each fora ... | 1992 | 1312526 |
| influence of hay making on in situ nitrogen degradability of forages in cows. | the influence of hay making on in situ n degradability of forages was assessed by comparing the degradability of nine hays with that of standing forages at the time of harvest. three ryegrass, two cocksfoot, and three alfalfa forages were used. the n degradability of hays was related closely to that of the corresponding standing forages (r = .91) and was, on average, lower (-2.5 points). this decrease in degradability after hay making was not constant; it increased when the degradability of the ... | 1992 | 1314856 |
| variability of crossreactivity of ige antibodies to group i and v allergens in eight grass pollen species. | crossreactivity to dactylis glomerata, festuca rubra, phleum pratense, anthoxanthum odoratum, secale cereale, zea mays, and phragmites communis of ige antibodies against lol p i or lol p v was investigated by means of rast-inhibition. within a group of sera the degree of crossreactivity was demonstrated to be highly variable. individual sera were not always equally crossreactive to all pollen species. a high degree of crossreactivity for group i allergens did not necessarily implicate the same f ... | 1992 | 1382820 |
| [conditions required for chemical binding of dactylis glomerata grass pollen to inert stable carriers]. | the results of the direct rast (radio-allergo-sorbent-test) procedure used to standardise own-prepared pollen extract of the dactylis glomerata grass are presented. based on the performed standardisation, a system was set up for binding allergens to the stable inert carriers. the results of examination of the quality of d. glomerata allergen, bound covalently to paper disc, are presented also. evaluation was made in inep laboratories by comparing the d. glomerata allergen-bound discs and the cor ... | 2015 | 1481465 |
| [sensitivity and specificity of the determination of ige using chemiluminescence (cla allergy test)]. | we studied 80 patients. all patients underwent a thorough clinical evaluation, including a comprehensive study and physical examination of the ear, nose, throat and chest. each patient underwent prick testing and a nasal provocation test. in addition, blood was drawn for the allergen-specific ige test (rast) and total serum ige levels. we selected 11 allergens for this study: grass (dactylis glomerata-orchard grass, phelum pratense-timothy grass, lolium-raygrass), weeds (chenipodium, parietaria) ... | 1992 | 1509983 |
| recombinant pollen allergens from dactylis glomerata: preliminary evidence that human ige cross-reactivity between dac g ii and lol p i/ii is increased following grass pollen immunotherapy. | we previously described the isolation of three identical complementary dna (cdna) clones, constructed from orchard/cocksfoot grass (dactylis glomerata) anther messenger rna (mrna), expressing a 140,000 mw beta-galactosidase fusion protein recognized by ige antibodies in atopic sera. partial nucleotide sequencing and inferred amino acid sequence showed greater than 90% homology with the group ii allergen from lolium perenne (lol ii) indicating they encode the group ii equivalent, dac g ii. wester ... | 1992 | 1526648 |
| forage systems for beef production from conception to slaughter: i. cow-calf production. | six year-round, all-forage, three-paddock systems for beef cow-calf production were used to produce five calf crops during a 6-yr period. forages grazed by cows during spring, summer, and early fall consisted of one paddock of 1) tall fescue (festuca arundinacea schreb.)-ladino clover (trifolium repens l.) or 2) kentucky blue-grass (poa pratensis l.)-white clover (trifolium repens l.). each of these forage mixtures was combined in a factorial arrangement with two paddocks of either 1) fescue-red ... | 1992 | 1548221 |
| group v allergens in grass pollens: iv. similarities in amino acid compositions and nh2-terminal sequences of the group v allergens from lolium perenne, poa pratensis and dactylis glomerata. | monoclonal antibodies (ppv4) raised against phleum pratense group v allergen were used for immuno-affinity chromatography of cross-reacting group v allergens from related grass species. fractions enriched in group v allergen were obtained from lolium perenne, poa pratense and dactylis glomerata extracts. the major components in these fractions were found in the mwr range 25-28 kd. ige binding to these components was shown using a pool of grass allergic sera, by sds-page immunoblotting. these fra ... | 1992 | 1611548 |
| n-terminal amino acid sequence homologies of group v grass pollen allergens. | in an earlier study, we presented data regarding the immunoaffinity purification and n-terminal sequencing of a major pollen allergen from orchard/cocks-foot grass (dactylis glomerata), now identified as the group v allergen dac g v. in this paper, we have extended our investigations to include group v allergens from other grass species. our data confirm the presence of group v-restricted characteristic n-terminal amino acid sequences containing a high alanine and hydroxyproline (p') rather than ... | 1992 | 1643443 |
| cocksfoot grass pollen allergens and genetic of immune response. | the genetic basis of allergic response to grass pollen allergens of 23 portuguese families have been studied. the two or three generations families formed a total of 128 individuals including at least one parent and one child sensitive to dactylis glomerata grass pollen. hla class ii genes were studied by pcr amplification and rflp analysis. our population study has revealed a higher frequency of allele 2 of dob in the allergic population than in non allergic individuals. 60% of cocksfoot sensit ... | 1991 | 1811648 |
| rast in diagnosis and therapy of allergic rhinitis. | skin tests, rast, nasal provocation tests, and other nasal allergy examinations were performed on 463 patients exhibiting symptoms of allergic rhinitis who were examined at the department of otolaryngology of osaka city university medical school, and comparisons were made between the rast scores and the results of the other examinations. a study was also made of the change in grade of the allergic rhinitis and the change of the rast score resulting from one year of immunotherapy. the rates of ag ... | 1991 | 1842868 |
| antigenic activity of nonpollen parts (leaves and stems) of allergenic plants (parietaria judaica and dactylis glomerata). | in patients with respiratory allergy to pollen it is common to correlate the onset, duration and intensity of clinical symptoms with the count of atmospheric allergenic pollen grains. pollen counts, however, may not reflect the total airborne allergen exposure since previous data suggest that pollen allergens may also be carried in microaerosol suspensions. these microdroplets may penetrate deeply into the airways, where pollen grains are too large to penetrate, eventually inducing asthma. the o ... | 2005 | 1952298 |
| characterization of the major allergen of cynodon dactylon (bermuda grass) pollen, cyn d i. | an allergen from cynodon dactylon (bermuda grass) pollen, cyn d i, has been purified by a combination of concanavalin a-sepharose affinity chromatography, and carboxymethyl-sepharose chromatography. the allergen constitutes the major allergenic component of the pollen extract as observed by immunoelectrophoretic techniques, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, an ige-inhibition experiment, and skin testing. in sodium dodecyl sulfate-polyacrylamide gel electrophoresis, cyn d ... | 1991 | 1955635 |
| monoclonal antibodies to proteins from cocksfoot grass (dactylis glomerata) pollen: isolation and n-terminal sequence of a major allergen. | monoclonal antibodies (mabs) raised to an aqueous extract of cocksfoot grass (dactylis glomerata) pollen have been characterised. mab 1b9 was demonstrated by sds-page and western blotting to recognise a major allergen of an approximate molecular weight of 28 kd in this extract, termed dg3, and a component with a molecular weight of between 35 and 40 kd in an extract of secale cereale (cultivated rye) pollen. the 28 kd component of cocksfoot grass pollen isolated by affinity chromatography using ... | 1990 | 2210876 |
| immunotherapy with alginate-conjugated two grass pollen extract in patients with allergic rhinoconjunctivitis. | the aim of the present study was to evaluate the clinical and immunological effects of specific immunotherapy with alginate-conjugated two grass pollen extract in patients with rhinoconjunctivitis. a total of 52 patients were admitted to the study. before and after 3 years of immunotherapy quantitative skin test (qst) to dactylis glomerata (dg) and to phleum pratense (php), variation of grass specific ige and igg4 and nasal challenge with the aqueous extract of the two grasses were compared. eac ... | 2015 | 2382596 |
| production of a monoclonal antibody against a major allergen of dactylis glomerata pollen (dg1). | a mouse monoclonal antibody was produced against the most frequent allergen ag dg1ief, purified from the dactylis glomerata grass pollen by isoelectric focusing. using the immunoprint technique, this monoclonal antibody detected ag dg1ief in d. glomerata pollen and some cross-reacting molecules in four other grass pollens. this hybridoma was grown in ascitic fluid. an immunosorbent was made from it and, by affinity chromatography, was used to purify monoclonal ag dg1m from the pollen extract. ag ... | 1985 | 2408551 |
| identification of orchard grass (dactylis glomerata) pollen allergens following electrophoretic transfer to nitrocellulose. | orchard grass (cocksfoot) pollen extracts, fractionated by polyacrylamide gradient electrophoresis or sds gel electrophoresis were electroblotted onto nitrocellulose membranes and probed with sera from orchard grass pollen-allergic patients and 125i-anti-human ige. the ige-binding components of the pollen were detected by autoradiography. elution studies showed that allergens could be extracted immediately and continuously over a 3-hour period. two fractions of mws 28,000 and 30,000 could be det ... | 1985 | 2411668 |
| purification and characterization of a major allergen from dactylis glomerata pollen: the ag dg1. | we have isolated an allergen (ag dg1) from dactylis glomerata pollen which is recognized by the serum of 95% of human patients sensitive to d. glomerata pollen, as has been shown by the nitrocellulose immunoprint technique. after two successive purifications by preparative isoelectric focusing (ief), dg1 was characterized as a single band in analytical agarose ief with a pi of 5.9 and was found to display 3 bands by sodium dodecyl sulfate polyacrylamide gel with the respective molecular weights: ... | 1985 | 2414230 |
| thermoinactivation of human ige: antigenic and functional modifications. | the thermoinactivation kinetics of ige were studied in experimental models revealing the antigenic properties and the basophil-sensitizing capacity of these immunoglobulins. a pool of human sera containing anti-dactylis glomerata (dg) ige was heated from 5 min up to 4 hr at 56 degrees. the ige antigenicity was tested by two polyclonal 125i-labelled anti-ige antibodies; one anti-ige was specific of the whole fc epsilon region, while the other had a specificity restricted to the d epsilon 2 domain ... | 1986 | 2420711 |
| study of the epitope structure of purified dac g i and lol p i, the major allergens of dactylis glomerata and lolium perenne pollens, using monoclonal antibodies. | the use of mab allowed us to further analyze the cross-reactivity between purified dac g i and lol p i, the major allergens of dactylis glomerata (cocksfoot) and lolium perenne (rye grass), respectively. it was first shown, using ief, followed by immunoprinting, that serum ige antibodies from most grass-sensitive patients recognize both dac g i and lol p i. second, three different anti-lol p i mab, 290a-167, 348a-6, and 539a-6, and one anti-dac g i mab, p3b2 were all shown to react with dac g i ... | 1988 | 2460536 |
| [skin reactivity to grass pollen and the mite dermatophagoides pteronyssinus in the prick-test using 2 types of allergen preparations]. | the reliability of prick testing with allergen preparations produced in yugoslavia was assessed by comparison to the internationally accepted ones (pharmacia diagnostica) of 100,000 bu activity. the following allergens were used: the mite dermatophagoides pteronyssinus in 107 subjects, and grass pollens: dactylis glomerata, poa pratensis, phleum pratense and secale cereale in 39 subjects. the clinical significance of the response was assessed in relation to the skin reaction to the negative cont ... | 1989 | 2638564 |
| relationship between skin-prick tests, the multiple allergosorbent test and symptoms of allergic disease. | in 47 atopic subjects, skin-prick testing to 10 common allergens was performed, and specific ige to the same allergens was assessed by the multi-allergosorbent chemiluminescent assay (mast-cla). overall agreement between the tests was 66.4% for conventionally positive skin tests (weal diameter greater than or equal to 3 mm), rising to 78.5% when a positive skin test was defined as having a weal diameter greater than or equal to 5 mm. agreement between the tests was statistically significant for ... | 1989 | 2649214 |
| the effects of intake of lucerne (medicago sativa l.) and orchard grass (dactylis glomerata l.) hay on the motility of the forestomach and digesta flow at the abomaso-duodenal junction of the sheep. | 1. the relations between food intake, reticulo-ruminal motility and abomasal digesta outflow were investigated in ewes receiving lucerne (medicago sativa) v. orchard grass (dactylis glomerata) hay. abomasal digesta outflow was recorded continuously by means of an electromagnetic flowmeter probe inserted into a duodenal t-shape cannula and the motility using strain-gauge force transducers. volumes and turnover rates of both rumen and abomasal liquid phases were measured by credta dilution. 2. the ... | 1989 | 2758019 |
| characterization of grass pollen-specific ige, iga, igm classes and igg subclasses in allergic patients. | water-soluble grass pollen extracts (dactylis glomerata) were separated by isoelectric focusing in a wide ph range (2-11) in agarose gel. after focusing, two successive gel prints were taken. the first one, on an ordinary nitrocellulose filter during 10 s, enabled the visualization of separated components of the pollen, after india ink staining. the second one, on a cyanogen bromide-activated nitrocellulose filter obtained after a 10-min transfer and saturation step, was incubated overnight with ... | 1989 | 2759718 |
| cloning of cdna coding for an allergen of cocksfoot grass (dactylis glomerata) pollen. | messenger rna isolated from cocksfoot grass (dactylis glomerata) anthers has been used to generate a cdna library in lambda t11. three cdna clones (7.8, 8.1, and 8.3) were demonstrated to be recognized by human ige antibodies in atopic serum and by rabbit polyclonal antiserum raised to a crude aqueous extract of cocksfoot pollen. the size of the cdna inserts was determined as approximately 700 bp, and restriction mapping demonstrated them to be identical sequences. lysogens obtained in escherich ... | 1989 | 2807580 |
| characterization with monoclonal and polyclonal antibodies of a new major allergen from grass pollen in the group i molecular weight range. | we have purified a 24/25 kd allergen from orchard grass pollen (dactylis glomerata) that has an allergenic potency similar to that of the major group i allergen. we provisionally named this allergen grass 4b1 after the monoclonal antibody used for its identification and purification. this monoclonal antibody was obtained by immunizing mice with whole lolium perenne-pollen extract and by screening the antibody producing hybrids for reactivity with dactylis glomerata-pollen extract. grass 4b1 is p ... | 1989 | 2913133 |
| selection among nonlinear models for rate of passage studies in ruminants. | eight toggenburg wether goats were fed either coastal bermudagrass (cynodon dactylon) or orchardgrass (dactylis glomerata)-alfalfa (medicago sativa) hay (57:43%) in a reversal design. four markers were used to study the movement of different digesta fractions: erbium for large particles greater than 1,700 microns; ytterbium for medium particles less than 1,700 microns and greater than 500 microns; chromium for small particles less than 500 microns and greater than 32 microns; and cobalt to follo ... | 1988 | 3225248 |
| [identification of orchard grass (dactylis glomerata) pollen allergens by immunoblotting]. | 1988 | 3228359 | |
| effect of ruminal infusion of glucose, volatile fatty acids and hydrochloric acid on mineral metabolism in sheep. | two experiments were conducted to study the effects of alterations in ruminal ph and volatile fatty acid (vfa) concentrations on utilization of mg and other minerals. in exp. 1, two metabolism trials were conducted with 12 ruminally cannulated crossbred wethers fed 800 g/d of orchard-grass (dactylis glomerata, l.) hay. after each feeding, wethers were ruminally infused with 500 ml (4.2 ml/min) or either 1) deionized water, 2) 40% (w/v) glucose solution, 3) .26 m propionic and .17 m butyric acid ... | 1988 | 3372390 |
| utilization of magnesium and other macrominerals in sheep supplemented with different readily-fermentable carbohydrates. | sheep were used to determine the effects of dietary supplementation with readily-fermentable carbohydrates on mg, ca, k and p utilization. in each of two metabolism trials, 15 mature, crossbred wethers (average weight, 49.2 kg) were allotted to five dietary treatments consisting of 800 g/d of orchardgrass (dactylis glomerata, l.) hay alone, or supplemented with 450 g/d of either glucose, sucrose, lactose or starch. each trial consisted of a 5-d adjustment period, a 10-d preliminary period and a ... | 1987 | 3443586 |
| [comparative study of various technics for measuring serum ige levels]. | in this work we report the results of a comparative study of different techniques to measure specific ige in sera. three methods were used, phadebas rast, phadezym rast (pharmacia) and allergen-specific fast+ test (3m diagnostics): 127 sera were tested with several pneumoallergens (housedust, dermatophagoides pteronyssinus, dactylis glomerata pollen, cat and dog hair). analysis of the results shows the following correlations: phadebas and phadezym rast 92%; phadebas rast and fast+ 88%; phadezym ... | 1987 | 3453730 |
| a comparison of two grass pollen extracts in the treatment of hay fever. | this trial of hyposensitisation compares the efficacy and safety of an alum absorbed 2-grass extract (phleum pratense and dactylis glomerata) with a traditional pyridine extracted alum precipitated 5-grass preparation whose efficacy has been demonstrated previously. 33 patients, (20 alpare, 13 allpyral), were entered into the trial which continued for 3 years; in the second year there were 17 alpare and 11 allpyral patients, and 13 alpare and 10 allpyral in the third year. patients on alpare req ... | 2006 | 3618442 |
| preliminary experimental and clinical results with inactivated allergens conjugated to the corynebacterium granulosum-derived immunomodulator p40. | allergoids have been used successfully for immunotherapy of allergic disorders. it has appeared to us that the effect of allergoids could be potentiated by their coupling to an immunomodulator. in the present study we show that a conjugate made up of the coupling of ovalbumin through glutaraldehyde action to the c. granulosum-derived immunomodulator p40 is completely devoid of antigenicity and of cross-reactivity with ovalbumin. this conjugate was found to significantly inhibit mast cell degranu ... | 1987 | 3620123 |
| rast inhibition: a collaborative study on its suitability and reproducibility for the assay of cocksfoot pollen extracts. | the suitability and reproducibility of the inhibition mode of the radioallergosorbent test (rast) as a method for the assay of allergen extracts was examined in a collaborative study involving seven laboratories. participants assayed five coded extracts of cocksfoot grass (dactylis glomerata) pollen, including three batches from one manufacturer, both by a common method and by their local methods of rast inhibition. for the versions of rast inhibition employed in this study the estimates of rela ... | 2013 | 3719934 |
| comparison between rush immunotherapy with a standardized allergen and an alum adjuved pyridine extracted material in grass pollen allergy. | rush immunotherapy with a standardized and lyophilized cocksfoot pollen extract was performed in twenty-three allergic subjects and compared with classical immunotherapy done with an alum adjuved, pyridine extracted cocksfoot pollen extract (fifteen subjects) and a control group of ten patients. the three groups were perfectly matched. clinical benefits were analysed by means of symptom scores. it was shown that rush immunotherapy gave significantly better results when asthma or rhinitis was con ... | 1985 | 3888440 |
| comparison of the stability of a mite and a pollen extract stored in normal conditions of use. | pollen extracts are stable in solutions containing 50% glycerol. the stability of dermatophagoides pteronyssinus (dp) extracts have not been determined. two lyophilised extracts (dp and cocksfoot pollen) were reconstituted in 50% glycerol and stored for up to 6 months at +4 degrees c. every day the extracts were placed at room temperature for 1 hr to mimic the natural use of allergen extracts by allergists. every 2 months the extracts initially prepared were compared to freshly reconstituted ext ... | 1985 | 3978775 |
| induction of a passive cutaneous anaphylaxis inhibitory factor in mice. | the administration of a purified protein from dactylis glomerata pollen, named dactylis inhibitory protein (dip), to balb/c mice results in the production of a heat-resistant seric factor that blocks the passive cutaneous anaphylaxis (pca) reaction in rat skin. this pca inhibitory factor (pca-if) was purified following different purification procedures and analyzed in sodium dodecyl sulfate polyacrylamide gel. amongst the array of bands detected it was possible to ascribe the inhibitory activity ... | 1985 | 4055080 |
| critical evaluation of an indirect enzyme-linked immunosorbent assay for igg antibodies to dactylis glomerata. | experiments are reported for the evaluation of an indirect elisa using peroxidase conjugate for the detection of igg antibodies to dactylis glomerata (dg), a grass pollen antigen frequently responsible for allergic rhinitis and asthma. when optimized, the test proved to be useful in clinical practice for a semiquantitative, cheap and simply-performed determination of igg antibodies to dg. the advantages and disadvantages of this method, compared with those of elaborate antigen-binding assays, ar ... | 2007 | 4081522 |
| [enzootic calcinosis in cattle. 5. experimental studies on rabbits selectively fed with ball-grass (dactylis glomerata), gold-oats (trisetum flavescens) and a grass mixture]. | 1972 | 4551838 | |
| cocksfoot mild mosaic virus--phleum mottle virus: a comparison. | 1974 | 4851066 | |
| [the occurrence of the cocksfoot streak virus]. | 2016 | 4912699 | |
| metabolic inhibitor(s) in orchardgrass (dactylis glomerata l.). | 1968 | 5690265 | |
| the energy value of ryegrass and cocksfoot assessed by a slaughter technique with lambs. | 1965 | 5835395 | |
| metabolic inhibitor(s) in fractions of orchardgrass (dactylis glomerata l.) detected by in vitro rumen fermentation technique. | 1967 | 6024807 | |
| mineral status of beef cows and sheep on spring pasture fertilized with kieserite. | the utilization of minerals in orchardgrass (dactylis glomerata l.) fertilized with kieserite (2,240 kg/ha) was examined in spring with lactating beef cows and wether lambs. kieserite increased the concentration of mg in vegetative herbage from .22 to .29% ( p less than .01) and of s from .20 to .29% (p less than .01), with little effect on other minerals. fertilization also increased (p less than .01) apparent absorptions of mg and s by lambs fed cut orchardgrass, from 24.5 to 36.5% and from 58 ... | 1981 | 6263852 |
| presence of il 1 activity in the supernatant of human monocytes challenged with purified allergenic constituents. | purified allergenic constituents from dermatophagoides farinae mite (df 11 and df 6) and from orchard grass (dactylis glomerata) pollen (f 34) are able to trigger, in the absence of ige and igg antibodies, human adherent cells, leading to the secretion of interleukin 1, (il 1). this secretion was observed with adherent peripheral blood mononuclear cells from normal donors as well as from atopic patients. the il 1 activity was evaluated by the mice thymocyte mitogenesis in the presence of subopti ... | 1984 | 6332080 |
| isopycnic centrifugation of plant viruses in nycodenz density gradients. | isopycnic centrifugation of plant viruses in density gradients prepared from a new non-ionic medium (nycodenz) was investigated. particle density of luteo-, tymo-, nepo-, cocksfoot mild mosaic, tobamo-, hordei-like, potex- and potyviruses in buffered solutions of nycodenz ranged between 1.23 and 1.28 g/ml and did not strictly reflect their nucleic acid and protein composition. isopycnic centrifugation of several partially purified viruses yielded preparations which were pure by electron microsco ... | 1984 | 6520198 |
| investigations of the allergens of cocksfoot grass (dactylis glomerata) pollen. | the pollen of cocksfoot grass (dactylis glomerata) is an important cause of allergic reactions in man. preliminary studies, which established that constituents of an extract of this pollen could be separated, and then recovered efficiently, by size-exclusion chromatography on tsk g3000 sw, have been extended. a comparative examination has been made by this procedure of cocksfoot pollen extracts from different sources and of several batches of extract from one source. the recovery and distributio ... | 1983 | 6630355 |
| alkaloids in ergot found on different gramineae in the netherlands. | the alkaloid content and the composition of the alkaloid complex of thirteen samples of ergot sclerotia from different gramineous host species collected in the netherlands were investigated. two samples collected in france were also examined. ergot of glyceria fluitans (l.) r.br. did not contain alkaloids. the total alkaloid content of ergot found on the other wild grasses was more than 0.2%. the ratio between the contents of water soluble and water insoluble alkaloids of the investigated ergot ... | 1983 | 6646990 |
| a report on the consumption, composition and nutritional adequacy of a mixture of lush green perennial ryegrass (lolium perenne) and cocksfoot (dactylis glomerata) fed ad libitum to thoroughbred mares. | non-pregnant thoroughbred mares were stabled and subjected to 2 trials, each 24 h in duration, to establish their total consumption of a mixture of freshly cut, lush green perennial ryegrass (lolium perenne) and cocksfoot (dactylis glomerata) in approximately equal proportions; and to compare the total intake of crude protein, calcium, phosphorus and mass of the grass mixture on a dry matter basis with their daily nutritional requirements. the body mass of each mare was calculated at the commenc ... | 1983 | 6655653 |
| a collaborative study on rast inhibition using a common method. | a collaborative study was carried out to determine the reproducibility of the inhibition form of the radioallergosorbent test (rast). seven laboratories were provided with three coded extracts of cocksfoot (dactylis glomerata) pollen. all laboratories used a common method of rast inhibition and were supplied with all reagents required to carry out the assays. in addition, four laboratories used local methods of rast inhibition. potency estimates were in reasonable agreement between laboratories ... | 1984 | 6697468 |
| immunotherapy with alginate-conjugated and alum-precipitated grass pollen extracts in patients with allergic rhinoconjunctivitis. | in order to compare the safety and the efficacy of two grass pollen extracts made from the same starting material, i.e. equal proportions of cocksfoot and timothy, a 3-year study was made on patients with hay fever. one product was a dialysed aqueous extract of pollens which was chemically conjugated to activated sodium alginate ( conjuvac ). the other was a pyridine-extracted alum-precipitated crude extract (allpyral). forty patients with classical hay fever were admitted to the study. all gave ... | 1984 | 6731753 |
| heterogeneity of grass pollen allergens (dactylis glomerata) recognized by ige antibodies in human patients sera by a new nitrocellulose immunoprint technique. | a new nitrocellulose immunoprint technique has been developed to detect specific antigens or/and allergens present among a heterogeneous solution such as a water-soluble crude extract of a grass pollen (dactylis glomerata). the antigens are separated by isoelectric focusing (ief) in an agarose gel and characterized by their isoelectric point (pi). these antigens are transferred and immobilized on a nitrocellulose sheet. they are recognized by the binding of specific antibodies contained in an un ... | 1982 | 6759375 |
| performance of dairy cows in midlactation fed high quality grass pasture and concentrate at three percents of energy requirements. | thirty multiparous cows rotationally grazed high quality grass pastures (predominantly dactylis glomerata and poa pratensis) for 140 days. stage of lactation was 54 to 192 days at beginning of trial. cows were offered concentrates to supply 33, 66, or 100% of requirements for net energy for lactation above maintenance; daily intakes by the three groups averaged 3.5, 7.7, and 11.5 kg. refusal of concentrate tended to increase with supplementation. average milk production was 19.2, 20.4, and 21.5 ... | 1983 | 6841749 |
| inhibition of the passive cutaneous anaphylaxis reaction to dactylis glomerata pollen allergens by a purified component of this pollen. | by isoelectric focusing a protein fraction of pi 4.6 was isolated from a crude water-soluble extract of dactylis glomerata pollen (se). this fraction was neither immunogenic nor allergenic in balb/c mice. in one week, this protein inhibited the mouse ige-specific antibodies to the soluble extract as measured by pca in rats and was therefore called dactylis inhibitory protein (dip). two experimental approaches which lowered ige anti-se titer were undertaken. pretreatment with dip as well as injec ... | 1983 | 6885115 |
| [reduced allergen immunotherapy of grass pollinosis (author's transl)]. | an excellent model for investigating the maximization of specific immunotherapy is grass pollen allergy. treatment is more effective when the number of component antigens used are reduced. we know that rye and cocksfoot pollen carry major antigens of grass pollen. a controlled clinical study was carried out with 128 patients divided into 5 groups as follows: group 1 - treatment with a mixture of 7 grass pollen extracts (allpyral). group 2 - treatment with a cocksfoot pollen extract (allpyral). g ... | 1980 | 6936982 |
| grass pollen hyposensitization versus placebo therapy. i. clinical effectiveness and methodological aspects of a pre-seasonal course of desensitization with a four-grass pollen extract. | the present work reports the results of a double-blind clinical trial, comparing the effects of hyposensitization treatment versus placebo in 33 patients with allergic rhinitis, sensitive to a crude extract of the pollen of four different grasses (dactylis glomerata, lolium perenne, secale cereale, and phleum pratense). the distribution of these patients in the two groups was done randomly and gave two comparable groups, as far as clinical and biological features are concerned. the treatment cou ... | 1981 | 7032341 |
| [evolution of microflora during the conservation of forage in microsilos]. | fresh and wilted samples of lolium italicum a. br., dactylis glomerata l., medicago sativa l., have been ensiled in laboratory microsilos for the study of microflora evolution. the principal microbial groups (coliforms, proteolytic bacteria, clostridia, lactic acid bacteria, yeasts) have been detected and enumerated, in anaerobic atmosphere, after 8, 15, 22, 29, 36, 180 days of ensiling. although the number of lactic acid bacteria resulted to the high in all the samples studied, they failed to p ... | 2006 | 7187351 |
| allergenic activity of fractions of cocksfoot (dactylis glomerata) pollen. definition of active components by skin testing, and inhibition rast. | with the combined use of skin tests, rast and isoelectric focusing, a potency profile of a cocksfoot ((dactylis glomerata) pollen extract was prepared. it was found that two fractions with pi 4.5 and 6.0 were the most allergenic, had the highest protein concentration and were also the most easily extracted. the importance of converting mean weal sizes into potency units when comparing the allergenicity of pollen fractions is emphasized. | 1981 | 7249343 |
| the relationship of certain tymoviruses assessed from the amino acid composition of their coat proteins. | the amino acid composition of the coat proteins of the following viruses is reported: andean potato latent, clitoria yellow vein, desmodium yellow mottle, dulcamara mottle, eggplant mosaic, okra mosaic, ononis yellow mosaic, scrophularia mottle, and, as controls, cocksfoot mild mosaic and cocksfoot mottle viruses. these data, together with some already published, were used to compute classifications of the tymoviruses. these classifications show a general similarity to koenig's serological class ... | 1980 | 7372445 |
| ige antibody formation in balb/c mice without adjuvant: induction of responses to grass pollen extract and to a hapten-carrier conjugate. | the ige antibody responses of inbred balb/c mice to subcutaneously injected antigens, given without adjuvant, were studied. cocksfoot grass pollen extract (gpe) (100-100,000 pnu) induced no detectable primary ige antibody responses but following second and subsequent injections, substantial secondary responses were produced. weekly injections for 9 weeks of doses of doses of 0.1-10,000 pnu gpe all produced similar ige antibody titres as measured by 24 h passive cutaneous anaphylaxis (pca) in rat ... | 1980 | 7380480 |
| [the monitoring of natural populations of dactylis glomerata l. in the area of the accident at the chernobyl atomic electric power station]. | results of five years monitoring (1986-1991) of seed quality (sq) and chlorophyll mutation frequency (mf) of dactylis glomerata l., growing within 30-km zone of chernobyl npp are presented. it was shown that during initial postaccident period (1986, 1987) the criteria depend of the gamma-phone on the sampling plots. it was not found the correlation between sq, mf and duration of plant growing under condition chronic irradiation. | 2016 | 7489103 |
| comparative studies of allergenic extracts prepared from freshly collected, dried, or dried and defatted pollen. | fresh pollen samples of parietaria judaica, betula pubescens, dactylis glomerata and olea europaea were collected and aliquot portions of these pollens were stored after drying in hot air, while other portions were dried and then defatted with diethylether. the different pollen materials were extracted with aqueous media to obtain the non-dialyzable allergenic protein constituents. review of the production data, together with comparative analyses of the uv-absorption spectra and of ige binding p ... | 1995 | 7582159 |
| characterization of cocksfoot mottle sobemovirus genomic rna and sequence comparison with related viruses. | the genome of cocksfoot mottle virus (cfmv) is a positive-sense ssrna molecule of 4082 nucleotides as revealed by sequencing the entire genome. the 5'-untranslated region of the genome is 69 nucleotides and the 3'-untranslated region is 225 nucleotides in length. the coding region contains four open reading frames (orfs). the organization of cfmv orfs differs significantly from that of the previously sequenced sobemoviruses southern bean mosaic virus and rice yellow mottle virus. orf1 encodes a ... | 1995 | 7595389 |
| optimization and characterization of freeze-dried multilamellar liposomes incorporating different standardized allergen extracts. | desensitization therapy for type i allergy is now current practice. liposomes have been proposed as a support for allergens to improve safety and effectiveness. the aim of this work was to optimize liposomal formulations of three different standardized allergen extracts and to test their allergenicity in vitro and in a preclinical trial. allergen extracts (dactylis glomerata, dermatophagoides pteronyssinus, and cat hair and dander) were associated with multilamellar liposomes of varying composit ... | 1994 | 7653744 |
| grass pollen immunotherapy induces highly cross-reactive igg antibodies to group v allergen from different grass species. | sera from two groups of patients receiving grass pollen immunotherapy were tested on igg reactivity with group v allergen from six different grass species. one group of patients was treated with a mixture of 10 grass species, and the other with a mixture of five. only lolium perenne, dactylis glomerata, and phleum pratense were present in both mixtures. although anthoxanthum odoratum and secale cereale were absent from the mixture of five, igg responses to ant o v and sec c v were comparable in ... | 1995 | 7677246 |
| replication and encapsidation of the viroid-like satellite rna of lucerne transient streak virus are supported in divergent hosts by cocksfoot mottle virus and turnip rosette virus. | cocksfoot mottle sobemovirus supports replication and encapsidation of the viroid-like satellite rna (sat-rna) of lucerne transient streak virus (ltsv) in two monocotyledonous species, triticum aestivum and dactylis glomerata. additionally, ltsv sat-rna replicates effectively in the presence of turnip rosette sobemovirus in brassica rapa, raphanus raphanistrum and sinapsis arvensis, but not in thlaspi arvense or nicotiana bigelovii, indicating that host species markedly influence this interactio ... | 1993 | 7682254 |
| isolation and characteristics of the protozoal and bacterial fractions from bovine ruminal contents. | four cows were fed once a day either a cocksfoot hay diet (h) or a diet consisting of 65% hay and 35% pelleted ground barley (hb). 15(nh4)2so4 was continuously infused into the rumen as a microbial marker and ruminal digesta samples were collected during the 24-h postprandial period for the isolation of liquid-associated protozoa and bacteria (lap, lab) and particle-associated bacteria (pab). there were marked differences between ruminal ph diurnal variations with diets h and hb. irrespective of ... | 1994 | 7730192 |
| the putative replicase of the cocksfoot mottle sobemovirus is translated as a part of the polyprotein by -1 ribosomal frameshift. | the polyprotein of cocksfoot mottle sobemovirus (cfmv) is encoded by two overlapping open reading frames (orf). the orf 2a codes for the putative vpg and serine protease and the orf 2b codes for the putative replicase. the consensus signals for a -1 ribosomal frameshifting event are found at the very beginning of the overlapping region of these orfs. the shifty heptanucleotide in cfmv is uuuaaac, and the secondary structure after the shifty sequence is predicted to be a stem-loop. in vitro trans ... | 1995 | 7886961 |
| seasonal asthma caused by airborne platanus pollen. | this work describes three patients suffering from bronchial asthma after being naturally exposed to airborne plane-tree pollen. the three patients gave immediate response in skin tests and dual response in bronchial provocations using platanus hybrida extract. there was specific seric ige activity against this/these antigen(s) with the cap system. the three patients also showed significant correlation (p < 0.001) between their rhinitis and asthma symptom-scores registered on their diary cards an ... | 1994 | 7889425 |
| evaluation of brassicas in grazing systems for sheep: i. quality of forage and animal performance. | four years of grazing trials were conducted with brassica forages to evaluate their chemical composition and effect on adg of fattening lambs and breeding ewes in late fall. brassicas tested included kales (brassica oleracea l. var. acephala dc), turnips (b. rapa l.), and a chinese cabbage hybrid (b. rapa l. x b. pekinensis [lour.] rupr.). daily gains of lambs varied widely among years (19 to 330 g/d); adg on brassicas were, however, generally higher than on stockpiled kentucky 31 tall fescue (f ... | 1994 | 7928762 |
| analysis of human t cell clones reactive with group v grass pollen allergens. | twenty-seven t cell clones (tcc) reactive with group v allergens of phleum pratense (phlp v) were established from the peripheral blood of 3 patients allergic to grass pollen. twenty-four tcc showed the helper cell phenotype cd3+, cd4+, cd8-; the remaining clones were cd3+cd4-cd8+. t cell recognition of phlp v was exclusively hla-dr restricted. many of the phlp v reactive tcc (19 of 27; 70%) were stimulated additionally by other group v allergens isolated from lolium perenne, poa pratensis, and ... | 1994 | 7981611 |
| comparison of three horizontal two-dimensional electrophoretic techniques to separate grass pollen allergens. | the identification and characterization of allergenic components are important for improving both diagnosis and therapy of allergy. we have studied grass pollen crude extract for better characterization of the allergen repertoire recognized by allergic patient ige antibodies. two-dimensional electrophoresis are the methods of choice to define physico-chemical characteristics of the allergens as they give isoelectric points and molecular mass of the analyzed samples. we compared the advantages an ... | 1994 | 8003933 |
| nucleotide sequence of cdna encoding the group ii allergen of cocksfoot/orchard grass (dactylis glomerata), dac g ii. | cocksfoot/orchard grass (dactylis glomerata) anther cdna clones encoding the group ii allergen dac g ii were previously isolated on the basis of immunoreactivity of human, rabbit, and murine antibodies with a 24-kda protein expressed as a fusion protein with beta-galactosidase. nucleotide sequencing reveals an open reading frame predicting expression of a 98-amino-acid (11-kda) polypeptide exhibiting > 90% homology with the group ii allergen of lolium perenne, lol p ii. in vitro translation of d ... | 1993 | 8116860 |
| digestibility, nitrogen utilization, and voluntary intake of ensiled crab waste-wheat straw mixtures fed to sheep. | crab waste and wheat (triticum aestivum l.) straw mixtures, ensiled with different additives, were evaluated in metabolism and palatability trials. crab waste and straw were mixed in proportions of 1:1, wet basis, with 20% water and different additives, and ensiled in 210-l metal drums double-lined with polyethylene bags. thirty crossbred wethers (40 kg initial bw) were fed a 1) basal diet consisting of 75% orchardgrass (dactylis glomerata l.) hay and 25% concentrate, 2) ensiled crab waste-wheat ... | 1994 | 8181970 |
| influence of forage species and diet particle size on the passage of digesta and nylon particles from the reticulorumen of steers. | to assess factors that influence the passage of digesta from the reticulorumen, ruminally fistulated steers (387 kg) were fed to appetite in a 4 x 4 latin square design either orchardgrass (og) (dactylis glomerata l.) or switchgrass (sg) (panicum virgatum l.) hays. the two hays were fed in either the long form or ground to pass a 2-cm screen. steers fed og diets had greater dmi (p < .05) than steers fed sg diets (11.7 vs 10.4 kg/d respectively). particle size of ruminal digesta and feces determi ... | 1993 | 8226378 |
| an integrated, dynamic model of feed hydration and digestion, and subsequent bacterial mass accumulation in the rumen. | hydration of feeds and bacterial attachment to feed particles are thought to play major roles in rumen digestion of fibrous feedstuffs. the objective of the present study was to integrate these phenomena in a mechanistic model that could be used for data analysis. the proposed model was based on the conversion of biomass, where digestion end-products can be used for the synthesis of bacterial mass. digestion of the potentially digestible fraction and subsequent accumulation of bacterial mass was ... | 1993 | 8260474 |
| dmisa (dissociated membrane immunosorbent assay), a new elisa technique performed with blotted samples. | this study describes the use of electrophoretically purified antigens blotted onto nitrocellulose, as solid phase antigens for enzyme-linked immunosorbent assays. this procedure is called dmisa, for dissociated membrane immunosorbent assay. the method is illustrated using immunoblotted antigens of dactylis glomerata grass pollen extract. the band of interest was located on a print of nitrocellulose by light staining (india ink), then the corresponding strip of nitrocellulose was cut out. immedia ... | 1994 | 8308278 |
| two-dimensional gel electrophoretic analysis with immobilized ph gradients of dactylis glomerata pollen allergens. | we studied orchard grass pollen allergens (dactylis glomerata) by a new two-dimensional electrophoretic technique which is the combination of isoelectric focusing (ief) in immobilized ph gradient (ipg) and horizontal sds-page. the ipg-dalt (two-dimensional gel electrophoresis with immobilized ph gradients in the first dimension) electrophoresis was made with four different but complementary ph gradients: 3-10.5, 4-9, 4-7, 6.3-10.5. we also compared the behavior of allergens in native and denatur ... | 1993 | 8400888 |
| purification of human igg4 subclass with allergen-specific blocking activity. | blocking antibodies (bab) induced by allergen immunotherapy are restricted to the igg1 and igg4 subclasses, with igg1 predominating early and igg4 coming later. study of igg4 bab has been limited, in part, by the absence of a method to purify igg4. we describe a rapid immunoaffinity chromatographic method for the purification of that subclass from whole serum. starting serum (tr) contained 90 micrograms/ml dactylis glomerata (orchard grass) pollen (dgp)-specific igg4, measured by indirect elisa. ... | 1993 | 8409473 |
| in vivo assessment of mast cell functional alteration induced by l-leucine methyl ester, using the passive cutaneous anaphylaxis technique. | we have recently reported that in vitro, mast cells were sensitive to the action of l-leucine methyl ester (leu-ome), a lysosomotropic compound. we now report the in vivo effect of leu-ome on mast cells, qualitatively assessed by using the passive cutaneous anaphylaxis (pca) reaction. the l- but not the d-stereoisomer of leu-ome (25 mm) injected together with dactylis glomerata, pollen-specific, ige-containing serum inhibited the pca reaction in rat skin triggered by a subsequent challenge with ... | 1993 | 8428165 |
| isotypic analysis of grass pollen-specific immunoglobulins in human plasma. 1. specialization of certain classes and subclasses in the immune response. | the specificity and isotypic profile of humoral immune responses to dactylis glomerata (cocksfoot) pollen was studied by isoelectric focusing (ief)-immunoprint analysis using 26 human plasma samples with high levels of dactylis pollen-specific igg4 (igg4+ plasma) and 25 human plasma samples with low levels of specific igg4 (normal plasma). over 60 individual protein components in an aqueous pollen extract were separated by ief and immunoprinted onto nitrocellose (nc). following plasma incubation ... | 1993 | 8428167 |
| allergenic relationship between taxonomically diverse pollens. | skin tests and tests for ige antibodies show that subjects are usually sensitive to a number of different pollens, frequently from taxonomically diverse species which are assumed to be allergenically non-crossreactive. this suggests that the presence of ige antibody-reactivity to an individual pollen may not necessarily have resulted from contact with that pollen or even with a taxonomically closely related species. | 1995 | 8521178 |
| t-cell epitopes of phl p 1, major pollen allergen of timothy grass (phleum pratense): evidence for crossreacting and non-crossreacting t-cell epitopes within grass group i allergens. | the use of peptides representing t-cell epitopes of allergens is a modern concept for improvement of specific immunotherapy. a prerequisite for this approach is the identification of t-cell epitopes of atopic allergens. | 1995 | 8543758 |
| spatial and temporal genetic variation in british field populations of the grain aphid sitobion avenae (f.) (hemiptera: aphididae) studied using rapd-pcr. | the grain aphid sitobion avenae (f.) was collected from winter wheat and adjacent cocksfoot grass at two locations in southern england and at four times in the year (april-july). genetic variation between individual aphids was then investigated using random amplified polymorphic dna polymerase chain reaction. individuals caught in wheat and cocksfoot during april provided very different and highly diagnostic banding patterns that were independent of location. this host-based genetic differentiab ... | 1995 | 8587889 |
| forage systems for beef production from conception to slaughter: iii. finishing systems. | fall-weaned angus calves grazed or were fed different forages during winter followed by 1) n-fertilized tall fescue (festuca arundinacea schreb.) grazed alone, 2) bluegrass (poa pratensis l.)-white clover (trifolium repens l.) sequence grazed with tall fescue-red clover (trifolium pratense l.), or 3) bluegrass-white clover sequence grazed with alfalfa (medicago sativa l.)-orchardgrass (dactylis glomerata l.). heifers were supplemented with grain at 1% of bw from april until slaughter in july. on ... | 1996 | 8707721 |