| identification of a sweet potato feathery mottle virus isolate from china (spfmv-ch) by the polymerase chain reaction with degenerate primers. | four degenerate oligonucleotide primers derived from conserved regions of the genome of potyviruses have been designed. a combined assay of reverse transcription and the polymerase chain reaction utilizing these primers on total rna extracted from ipomoea purpurea infected with a sweet potato feathery mottle virus isolate from china (spfmv-ch), amplified a 1.35 kb and a 830 bp fragment. these amplified fragments were cloned into the bluescript plasmid and sequenced. the comparison of the sequenc ... | 1993 | 8113341 |
| a large deletion in the plastid dna of the holoparasitic flowering plant cuscuta reflexa concerning two ribosomal proteins (rpl2, rpl23), one transfer rna (trni) and an orf 2280 homologue. | we have determined the nucleotide sequence of a 5.3-kb region of the plastid dna (ptdna) from the heterotrophic holoparasitic plant cuscuta reflexa. the cloned area contains genes for the d1-protein (32-kda protein; psba), trna(his) (trnh), orf 740 (homologous to orf 2280 from nicotiana tabacum), orf 77 (homologous to orf 70), trna(leu) (trnl) and a hypothetical orf 55 which has no homology to any known gene among higher plants. this 5.3-kb area is colinear with a 12.4-kb region of tobacco ptdna ... | 1993 | 8358824 |
| patterns of evolutionary rate variation among genes of the anthocyanin biosynthetic pathway. | the anthocyanin biosynthetic pathway is responsible for the production of anthocyanin pigments in plant tissues and shares a number of enzymes with other biochemical pathways. the six core structural genes of this pathway have been cloned and characterized in two taxonomically diverse plant species (maize and snapdragon). we have recently cloned these genes for a third species, the common morning glory, ipomoea purpurea. this additional information provides an opportunity to examine patterns of ... | 1999 | 10028292 |
| dynamics of mobile element activity in chalcone synthase loci in the common morning glory (ipomoea purpurea). | mobile element dynamics in seven alleles of the chalcone synthase d locus (chs-d) of the common morning glory (ipomoea purpurea) are analyzed in the context of synonymous nucleotide sequence distances for chs-d exons. by using a nucleotide sequence of chs-d from the sister species ipomoea nil (japanese morning glory [johzuka-hisatomi, y., hoshino, a., mori, t., habu, y. & iida, s. (1999) genes genet. syst. 74, 141-147], it is also possible to determine the relative frequency of insertion and los ... | 2001 | 11309503 |
| the transposon tip100 from the common morning glory is an autonomous element that can transpose in tobacco plants. | the mutable flaked or a (flaked) (a(f)) line of the common morning glory (ipomoea purpurea) displays white flowers with colored flakes, and the a(f) mutation is caused by the insertion of a transposable element named tip100 into the chs-d gene for anthocyanin biosynthesis. the 3.9-kb tip100 element belongs to the ac/ds family and contains an orf encoding a polypeptide of 808 amino acids. the frequency and timing of flower variegation vary in different a(f) lines, and a genetic element termed mod ... | 2002 | 11810246 |
| japanese morning glory dusky mutants displaying reddish-brown or purplish-gray flowers are deficient in a novel glycosylation enzyme for anthocyanin biosynthesis, udp-glucose:anthocyanidin 3-o-glucoside-2''-o-glucosyltransferase, due to 4-bp insertions in the gene. | bright blue or red flowers in the japanese morning glory (ipomoea nil) contain anthocyanidin 3-o-sophoroside derivatives, whereas the reddish-brown or purplish-gray petals in its dusky mutants accumulate anthocyanidin 3-o-glucoside derivatives. the dusky gene was found to encode a novel glucosyltransferase, udp-glucose:anthocyanidin 3-o-glucoside-2''-o-glucosyltransferase (3ggt), which mediates the glucosylation of anthocyanidin 3-o-glucosides to yield anthocyanidin 3-o-sophorosides. ipomoea nil ... | 2005 | 15842621 |
| occurrence of colombian datura virus in brugmansia hybrids, physalis peruviana l. and solanum muricatum ait. in hungary. | colombian datura virus (cdv) has been found to infect angel trumpets (brugmansia spp.) frequently and cape gooseberry (physalis peruviana) and pepino (solanum muricatum) sporadically in hungary. a cdv brg/h isolate was characterized. it had flexuous thread-like virions of about 750 x 12 nm in size. host range and symptomathological studies revealed its great similarity to authentic cdv isolates. nicotiana tabacum cultivars and lines resistant to potato virus y (pvyn) either genically or transgen ... | 2005 | 16047739 |
| summer weeds as hosts for frankliniella occidentalis and frankliniella fusca (thysanoptera: thripidae) and as reservoirs for tomato spotted wilt tospovirus in north carolina. | in north carolina, tomato spotted wilt tospovirus (family bunyaviridae, genus tospovirus, tswv) is vectored primarily by the tobacco thrips, frankliniella fusca (hinds), and the western flower thrips, frankliniella occidentalis (pergande) (thysanoptera: thripidae). tswv overwinters in winter annual weeds from which it is spread to susceptible crops in spring. because most susceptible crops are destroyed after harvest before winter weeds emerge in the fall, infected summer weeds are thought to be ... | 2005 | 16539098 |
| major-gene resistance to the rust pathogen coleosporium ipomoeae is common in natural populations of ipomoea purpurea. | the genetic basis of resistance to pathogens is well studied in crops, yet our understanding of the evolution of this trait in natural populations will be improved by determining how resistance is inherited in a wide range of plant-pathogen interactions. here, we examined resistance to coleosporium ipomoeae, a common fungal rust pathogen of ipomoea purpurea. natural populations across north carolina, south carolina, and georgia (usa) were surveyed for the presence of c. ipomoeae and seeds were c ... | 2006 | 16771989 |
| constraints on the evolution of tolerance to herbicide in the common morning glory: resistance and tolerance are mutually exclusive. | evolutionary biologists explain the maintenance of intermediate levels of defense in plant populations as being due to trade-offs, or negative genetic covariances among ecologically important traits. attempts at detecting trade-offs as constraints on the evolution of defense have not always been successful, leading some to conclude that such trade-offs rarely explain current levels of defense in the population. using the agricultural pest ipomoea purpurea, we measured correlations between traits ... | 2008 | 18786188 |
| the molecular basis of incomplete dominance at the a locus of chs-d in the common morning glory, ipomoea purpurea. | the mutable a(flaked (a(f)) allele at the a locus of the common morning glory (ipomoea purpurea) confers incomplete dominance in flower pigmentation and is caused by insertion of the dna transposon tip100 into chs-d, which encodes chalcone synthase and is required for anthocyanin biosynthesis. levels of chs-d transcripts, chs-d protein, and anthocyanin pigment in heterozygous flowers were about half that in homozygous flowers, indicating that dosage-dependent expression of chs-d is the primary c ... | 2011 | 20680382 |
| pathogen-induced systemic resistance in ipomoea purpurea. | infection of ipomoea purpurea by anthracnose, the disease caused by the fungal pathogen colletotrichum dematium, increases resistance to subsequent infections on previously uninfected leaves. fungal isolates varied in their levels of virulence but not in the extent to which they induced resistance. induced resistance was equally effective against all isolates tested. plant lines varied in the baseline level of resistance expressed in newly emerging leaves. in some lines, new leaves were poorly d ... | 1995 | 28306893 |
| natural association of two different betasatellites with sweet potato leaf curl virus in wild morning glory (ipomoea purpurea) in india. | wild morning glory (ipomoea purpurea) was observed to be affected by leaf curl and yellow vein diseases during summer-rainy season of 2009 in new delhi, india. the virus was experimentally transmitted through whitefly, bemisia tabaci to i. purpurea that reproduced the two distinct symptoms. sequence analysis of multiple full-length clones obtained through rolling circle amplification from the leaf curl and yellow vein samples showed 91.8-95.3% sequence identity with sweet potato leaf curl virus ... | 2013 | 23529301 |
| permanent genetic resources added to molecular ecology resources database 1 october 2012-30 november 2012. | this article documents the addition of 153 microsatellite marker loci to the molecular ecology resources database. loci were developed for the following species: brassica oleracea, brycon amazonicus, dimorphandra wilsonii, eupallasella percnurus, helleborus foetidus, ipomoea purpurea, phrynops geoffroanus, prochilodus argenteus, pyura sp., sylvia atricapilla, teratosphaeria suttonii, trialeurodes vaporariorum and trypanosoma brucei. these loci were cross-tested on the following species: dimorpha ... | 2013 | 23356940 |
| volatile metabolites controlling germination in buried weed seeds. | velvetleaf (abutilon theophrasti medic), morning glory (ipomoea purpurea [l.] roth), and wild mustard (brassica kaber [d.c.] l. c. wheeler) seeds exhibited decreased germination with increased planting depth in soil. flushing the soil for 2 minutes each day with air overcame the inhibition. a sealed in vitro system was used to sample the volatile components produced by weed seeds. inhibition of seed germination was accompanied by decreased o(2) levels and production of volatile metabolites ident ... | 1972 | 16658159 |
| characterization of the cis elements in the proximal promoter regions of the anthocyanin pathway genes reveals a common regulatory logic that governs pathway regulation. | cellular activities such as compound synthesis often require the transcriptional activation of an entire pathway; however, the molecular mechanisms underlying pathway activation have rarely been explained. here, the cis regulatory architecture of the anthocyanin pathway genes targeted by the transcription factor (tf) complex including myb, bhlh, and wdr was systematically analysed in one species and the findings extended to others. in ipomoea purpurea, the ipmyb1-ipbhlh2-ipwdr1 (ipmbw) complex w ... | 2015 | 25911741 |
| simultaneous identification of four "legal high" plant species in a multiplex pcr high-resolution melt assay(). | the international prevalence of "legal high" drugs necessitates the development of a method for their detection and identification. herein, we describe the development and validation of a tetraplex multiplex real-time polymerase chain reaction (pcr) assay used to simultaneously identify morning glory, jimson weed, hawaiian woodrose, and marijuana detected by high-resolution melt using lcgreen plus(®) . the pcr assay was evaluated based on the following: (i) specificity and selectivity-primers we ... | 2016 | 27957736 |
| comparative biochemical and immunological studies of the glycine betaine synthesis pathway in diverse families of dicotyledons. | members of the chenopodiaceae can accumulate high levels (>100 μmol·(g dw)(-1)) of glycine betaine (betaine) in leaves when salinized. chenopodiaceae synthesize betaine by a two-step oxidation of choline (choline→betaine aldehyde→ betaine), with the second step catalyzed by betaine aldehyde dehydrogenase (badh, ec 1.2.1.8). high betaine levels have also been reported in leaves of species from several distantly-related families of dicotyledons, raising the question of whether the same betaine-syn ... | 1989 | 24212901 |
| herbicidal activity of an isopropylmalate dehydrogenase inhibitor. | isopropylmalate dehydrogenase (ipmdh) is the third enzyme specific to leucine biosynthesis. it catalyzes the oxidative decarboxylation of 3-isopropylmalate (3-ipm) to 2-ketoisocaproic acid. the partially purified enzyme from pea (pisum sativum l.) shows a broad ph optimum of 7.8 to 9.1 and has km values for 3-ipm and nad of 18 and 40 [mu]m, respectively. o-isobutenyl oxalylhydroxamate (o-iboha) has been discovered to be an excellent inhibitor of the pea ipmdh, with an apparent inhibitor constant ... | 1994 | 12232331 |