PMID(sorted ascending)
alkaloids of vinca rosea l. (catharanthus roseus g. don). xxxvii. structure of vincathicine. 19763633
synthesis of ajmalicine and related indole alkaloids by cell free extracts of catharanthus roseus cell suspension cultures. 197611128
[fungal activity of various alkaloids isolated from catharanthus roseus g. don].evaluation is made of the fungal activity of ajmalicine, aparacine, catarantine, reserpine, tetrahydroalstonine, vincubine, vindoline and vindolinina--alkaloids isolated from c. roseus growing in cuba--on new fungi strains and yeasts which include some of human clinical interest. the method employed was the diffusion in an agar mean with sections or cylinders containing solutions of the alkaloids at 2% and 3% concentrations. the best results are obtained with an aparicine base, while tetrahydroa ...197798170
detergent-solubilized nadph-cytochrome c(p-450) reductase from the higher plant, catharanthus roseus. purification and characterization. 1998107158
5-methyltryptophan resistant cells of catharanthus roseus.several cell lines resistant to 5-methyltryptophan were selected from wild type cells of different catharanthus roseus suspension cultures. the resistant cells had up to 30 times the normal levels of free tryptophan. despite the increased pool size of tryptophan anthranilate synthetase activity of resistant cells was as sensitive to inhibition by l-tryptophan as wild type cells. the overproduction of tryptophan did not lead to intensified accumulation of tryptamine nor of indole alkaloids. this ...1979158896
[evaluation of antimicrobial activity of indol alkaloids].in pursuing the study of the antimicrobial properties of alkaloids prepared from cuban plants the activity of 10 indol alkaloids and 4 semisynthetic variables obtained from three plants--catharanthus roseus g. don., vallesia antillana wood and ervatamia coronaria staph, of the family apocynaceae--growing in cuba was assessed in vitro. the alkaloids and the variables used were catharantine, vindoline, vindolinine, perivine, reserpine, tabernaemontanine, tetrahydroalstonine, aparicine, vindolinic ...1979399043
a bioassay of antimitotic alkaloids of catharanthus roseus. 1979461560
purification and properties of strictosidine synthetase (an enzyme condensing tryptamine and secologanin) from catharanthus roseus cultured cells.strictosidine synthetase, which catalyzes the condensation of tryptamine with secologanin to form strictosidine (isovincoside), was purified 740-fold to homogeneity from cultured cells of catharanthus roseus in 10% yield. the specific activity is 5.85 nkat/mg. the molecular weight as estimated by gel filtration is 38,000. the isoelectric point is 4.6. apparent km values for tryptamine and secologanin are 0.83 and 0.46 mm, respectively. the enzyme shows a broad ph optimum between 5.0 and 7.5. the ...1976476085
purification and properties of strictosidine synthase, the key enzyme in indole alkaloid formation.a new enzyme, strictosidine synthase, which catalyzes the synthesis of 3-alpha(s)-strictosidine from tryptamine and secologanin was isolated from the soluble protein extract of catharanthus roseus cell suspension cultures and was purified approximately 50-fold by ammonium sulfate fractionation, column chromatography on deae-cellulose. ultrogel aca34 and isoelectric focusing. the apparent molecular weight of the enzyme was 34000. the ph optimum was 6.8, apparent km values for tryptamine and secol ...1979510306
[ajmalicine assay in catharanthus roseus g. don. a comparison between densitometric, spectrometric and hight performance liquid chromatographic methods (author's transl)]. 1979543596
alkaloids of vinca rosea l. (catharanthus roseus g. don). 38. 4'-dehydrated derivatives.a series of 4'-dehydrated derivatives of various dimeric vinca alkaloids has been synthesized to further define the structure-activity relationships of vinca alkaloids with onolytic potency. the concentrated sulfuric acid dehydration in most cases gave mixtures of the 3',4'-and two isomeric 4',20'-alkenes, which were isolated and characterized primarily by proton and 13c nmr. compound tested for antitumor activity include the three dehydro isomers of 4'-deacetylvinblastine, 4-deacetylvincristine ...1977576619
distribution and accumulation of alkaloids in catharanthus roseus g. don during development.pattern of the total alkaloids distribution and accumulation in catharanthus roseus g. don was investigated during six different stages of flowering and fruiting. the highest concentration of alkaloids (mg perivine/g dry weight) was found in the roots at the start of flowering and the minimum in the stems during full fruiting stage; the rate of alkaloidal accumulation in the leaves, stems and roots tended to decrease during fruit maturation. the most active stage of alkaloidal biosynthesis was a ...1978674322
[microbiological analysis of vindolinina (an alkaloid isolated from catharanthus roseus) and some of its structural changes].bacteriostatic properties of vindolinina (an alkaloid isolated from catharanthus roseus that has an ester group within its molecule) as well as of its alkaline-hydrolysis product (vindolininic acid) crystallized as chlorhydrate, and of the product of its reduction with lithium and aluminum hydrides (vindolininol) are compared. several strains of bacteria pathogenic to man (proteus, escherichia, shigella, staphylococcus, and pseudomonas) cultured in nutritive-agar dishes containing disks and incu ...1976802793
subcellular localization of a cytochrome p-450-dependent monogenase in vesicles of the higher plant catharanthus roseus.the intracellular location of a cytochrome p-450-dependent monoterpene hydroxylase from the higher plant, catharanthus roseus, has been investigated. by differential and sucrose density gradient centrifugation, utilizing marker enzymes and electron microscopy, the monooxygenase was demonstrated to be associated with vesicles having a membrane thickness of 40-60 nm. the vesicles could be distinguished from endoplasmic reticulum, golgi apparatus, mitochondria, and plasma membrane and were found in ...1977833200
indole aldaloid biosynthesis: partial purification of "ajmalicine synthetase" from catharanthus roseus. 1977861021
catharanthus roseus tissue culture: the effects of medium modifications on growth and alkaloid production.a number of nutritional factors as well as the growth factors 2,4-d and iaa were studied to determine their influence on growth and alkaloid formation in catharanthus roseus suspension cultures. the optimal 2,4-d concentration for growth and alkaloid production was 1 mg/liter. with iaa, both 0.5 and 2.0 mg/liter in media produced tissue growth comparable to tissue receiving 1 mg/liter 2,4-d; however, qualitative and quantitative differences in alkaloid production were observed. media formulation ...2013895391
[production of indole alkaloids in tissue cultures of catharanthus roseus (author's transl)]. 1976959385
biotransformations with plant tissue cultures.suspension cultures of catharanthus roseus, apocynum cannabinum and conium maculatum were examined for their capacity to transform aniline, anisole, acetanilide, benzoic acid and coumarin. none of the cultures transformed acetanilide but each produced acetanilide when fed aniline. all three cultures converted benzoic acid to its para-hydroxy derivative. coumarin was selectively hydroxylated at the 7-position by catharanthus and conium and anisole was o-demethylated only by older catharanthus tis ...20061084950
vinca alkaloids xxxv.1 desacetoxyvinblastine a new minor alkaloid from vinca rosea l. (catharanthus roseus g. don). 19751116520
letter: biosynthesis of the indole alkaloids. a cell-free system from catharanthus roseus. 19751184890
alkaloids of vinca rosea l. (catharanthus roseus g. don) xxxvi: isolation and characterization of new dimeric a continuing effort to study thoroughly the alkaloids of catharanthus roseus, new dimeric alkaloids were isolated and characterized. structures are proposed for leurocolombine and vinamidine based on uv, ir, pmr, high-resolution mass spectrometry, and cmr. pseudovincaleukoblastine diol was identified by pmr and mass spectrometry. leurocolombine exhibited antimitotic activity and marginal antitumor activity against the ridgeway osteogenic sarcoma.19751206487
purification of an indole alkaloid biosynthetic enzyme, strictosidine synthase, from a recombinant strain of escherichia coli.the gene for the indole alkaloid biosynthetic enzyme, strictosidine synthase, of catharanthus roseus has been cloned into an inducible escherichia coli expression vector using an expression cassette polymerase chain reaction technique. induction of the gene resulted in overexpression of the enzyme which accumulated mainly as insoluble inclusion bodies. denaturation and refolding of the insoluble protein resulted in the ability to purify up to 6 mg of active enzyme from a single liter of cell cul ...19921330135
monoclonal antibodies to bis-indole alkaloids of catharanthus roseus and their use in enzyme-linked immuno-sorbent-assays.high affinity monoclonal antibodies directed against bis-indole alkaloids from catharanthus roseus were produced. once characterized, they were used to develop sensitive enzyme-linked-immuno-sorbent-assays, enabling us to determine minute quantities of alkaloids related to vinblastine in plant material.19901366691
secondary metabolite biosynthesis in cultured cells of catharanthus roseus (l.) g. don immobilized by adhesion to glass fibres.suspension-cultured cells of catharanthus roseus (l.) g. don were immobilized on glass fibre mats and cultivated in shake flasks. the highly-aggregated immobilized cells exhibited a slower growth rate and accumulated reduced levels of tryptamine and indole alkaloids, represented by catharanthine and ajmalicine, in comparison to cells in suspension. the increased total protein synthesis in immobilized cells suggests a diversion of the primary metabolic flux toward protein biosynthetic pathways an ...19911367318
effect of culture process on alkaloid production by catharanthus roseus cells. i. suspension cultures.the processes for production of indole alkaloids in shake flask suspension cultures of catharanthus roseus cells using zenk's alkaloid production medium (apm) were evaluated. the 1-stage process consisted of inoculating apm and incubating for 15 days. the 2-stage process involved 6 d of cultivation in growth medium followed by 15 d of incubation in apm. growth, main nutrient consumption and alkaloid production were monitored. both culture processes produced approximately 20 g dw per 1 biomass. h ...19911367684
effect of culture process on alkaloid production by catharanthus roseus cells. ii. immobilized cultures.two processes for the production of indole alkaloids 2 l surface-immobilized bioreactor cultures of catharanthus roseus cells using zenk's alkaloid production medium (apm) were evaluated. the 1-stage process consisted of inoculating apm containing bioreactors and incubating for 15 d. the 2-stage process involved inoculating growth medium-containing bioreactors, growing the immobilized cultures for a certain period of time and subsequently replacing this medium with apm. the production stage whic ...19911367689
stimulated indole alkaloid release from catharanthus roseus immobilized cultures. initial studies.vacuolar sequestration of valuable secondary metabolites remains the major limitation to the use of immobilization technology for large scale plant-cell-based bioprocesses, which otherwise may be a more efficient culture system than suspension for this biomass. in this initial study, the release of indole alkaloids produced by immobilized catharanthus roseus cells cultured in zenk's alkaloid production medium was evaluated. unstimulated alkaloid release in immobilized cultures reached levels of ...19911367690
large-scale (20 l) culture of surface-immobilized catharanthus roseus cells.surface-immobilized c. roseus cell cultures were grown in a 20-l modified airlift bioreactor operated at 0.51 vvm (kla approximately 8 h-1) under various gassing regimes [air, 2% (v/v) and 5% co2]. extracellular ammonium, phosphate, and nitrate ions as well as carbohydrate uptake and ph value of the medium were monitored together with on-line dissolved oxygen concentration, conductivity of the medium, and carbon dioxide production rate (cpr) of the cultures. cultures supplemented with 2% co2 sho ...19911367999
biotransformation of tabersonine in cell suspension cultures of catharanthus investigate the reactions involved in the biosynthesis of vindoline from tabersonine, the bioconversion products formed when the latter compound was fed to cell suspension cultures of catharanthus roseus were isolated and characterized. two biotransformation products of tabersonine were isolated and shown to be lochnericine, which is formed by epoxidation of tabersonine at positions 14, 15, and lochnerinine, the 11-methoxylation product of lochnericine. the bioconversion ratio of the main bio ...19921368411
isolation of vindoline from catharanthus roseus by supercritical fluid extraction.vindoline was extracted from the leaves of catharanthus roseus over the ranges of 35-70 degrees c and 100-300 bar using supercritical carbon dioxide with and without the addition of 3 wt % ethanol as a cosolvent. the vindoline contents in the extracts were determined by hplc and identified by lc/ms. the remarkable highest vindoline concentration, 58 wt %, was obtained at the lowest temperature, 35 degrees c, and the highest pressure, 300 bar, of this study. the use of a cosolvent only slightly i ...20151369042
indole alkaloid formation by catharanthus roseus cells in a biofilm reactor.catharanthus roseus cells producing indole alkaloids were grown in the form of a biofilm. production medium was circulated through the reactor parallel to the upper surface of the horizontal biofilm. sugar consumption and indole alkaloid formation were followed to compare the performance of cultures with different biofilm thicknesses. dissolved oxygen concentrations gradients within the biofilms were determined at the end of each run. rna and protein content of the cells in the upper and lower l ...20061370003
identification of an essential histidine residue at the active site of the tonoplast malate carrier in catharanthus roseus cells.the involvement of a histidyl residue in the binding or translocation step was investigated in the malate carrier at the tonoplast of catharanthus roseus cells. the transport rate was strongly stimulated when the ph of the incubation medium was decreased from ph 7.0 to 5.0. the histidine-specific reagent diethylpyrocarbonate (depc) efficiently inhibited the activity of the malate carrier. inhibition developed rapidly and was completed after 5 min at a concentration of 2 mm depc. the original sub ...19921433274
a gene family homologous to the s-phase specific gene in higher plants is essential for cell proliferation in saccharomyces cerevisiae.previously we reported the isolation and characterization of the gene, cyc07, which was specifically expressed in the s phase during the cell cycle in synchronous cell division cultures of the higher plant, catharanthus roseus. we found that the yeast saccharomyces cerevisiae contains two closely related genes which show a high degree of similarity (about 64% at the amino acid level) to cyc07 of c. roseus. site-directed disruption mutations demonstrated that the two yeast genes, homologous to cy ...19921451783
the pharmacology of is impossible to predict what compounds of pharmacological interest may be present in an unexamined species. the extinction of such species may result, therefore, in the loss of therapeutically significant compounds. the fact that science will never know what has been lost does not lessen the significance of the loss. a number of species are discussed to exemplify the potential loss. ginkgo biloba is an ancient plant, apparently saved from a natural extinction by human intervention. from this ...19921453701
sensitive detection and identification of mycoplasma-like organisms in plants by polymerase chain reactions.dna amplification by polymerase chain reactions (pcr) was employed to detect host plant infection by several mycoplasmalike organisms (mlos), including the aster yellows (ay), dwarf aster yellows (day), and periwinkle little leaf (0-1) mlos. for pcr, two pairs of oligonucleotide primers, designated ay18pm and ay19pm, respectively, were synthesized on the basis of partial sequences of cloned ay mlo dna fragments ay18 and ay19. reaction mixtures containing primer pair ay18pm yielded a dna product ...19921510676
auxin rapidly down-regulates transcription of the tryptophan decarboxylase gene from catharanthus roseus.the enzyme tryptophan decarboxylase (tdc) (ec converts tryptophan into tryptamine, and thereby channels primary metabolites into indole alkaloid biosynthesis. the production of these secondary metabolites in suspension cells of catharanthus roseus depends on medium composition. of the possible variables, we investigated the effect of hormones on the expression of the tdc gene in cell cultures. omission of naa from the growth medium resulted in accumulation of tdc mrna. the addition of ...19921600147
coordinated regulation of two indole alkaloid biosynthetic genes from catharanthus roseus by auxin and elicitors.catharanthus roseus (periwinkle) produces a wide range of terpenoid indole alkaloids, including several pharmaceutically important compounds, from the intermediate strictosidine. the complete mrna sequence for the enzyme strictosidine synthase (sss) was determined. comparison of the primary structure of the encoded protein with the amino-terminal sequence of purified sss indicated the presence of a signal peptide of 31 amino acids in the putative primary translation product. sss is encoded by a ...19921600148
identification of a novel s-phase-specific gene during the cell cycle in synchronous cultures of catharanthus roseus cells.the cell-cycle specific cdnas were isolated from a cdna library prepared from cells in the s phase in the synchronous cultures of catharanthus roseus. one of the isolated genes, which we refer to as cyc07, was analyzed in detail. the full-length cdna of cyc07 contains an open reading frame of 735 nucleotides, encoding a protein of 245 amino acids with a molecular weight of 28,356 da. the protein predicted from the nucleotide sequence is highly basic, as are mammalian histones. cyc07 mrna was det ...19911844881
molecular cloning of the gene for plant proliferating-cell nuclear antigen and expression of this gene during the cell cycle in synchronized cultures of catharanthus roseus cells.a cdna library was screened for plant proliferating-cell nuclear antigen (pcna) from catharanthus roseus (periwinkle). a lambda gt11 cdna library was constructed using poly(a)-rich rna isolated from the cells in the s phase. a cdna clone for pcna was isolated by using a rice genomic clone, pcj-1, which contains pcna-related gene sequences. the cdna contains an open reading frame of 804 nucleotides, encoding a protein of 268 amino acids with a molecular mass of 29,765 da. when conservative substi ...19911902790
first field isolation of wound tumor virus from a plant host: minimal sequence divergence from the type strain isolated from an insect vector.a new strain of wound tumor virus (wtv) has been isolated from a periwinkle plant (catharanthus roseus) that was among several used as bait plants in a blueberry field. the 12 segments of double-stranded rna of the viral genome were isolated directly from infected tissue and found to have mobilities through agarose gels that were identical to those of the type strain wtv. coupled complementary dna (cdna) and polymerase chain reactions (pcr) primed with oligonucleotides complementary to the termi ...19911962460
isolation of catharanthus alkaloids by solid-phase extraction and semipreparative hplc.a combination of moderate-pressure chromatography on c18 sorbent and preparative hplc is developed for rapid isolation of alkaloids from catharanthus roseus. the procedure is optimized for vindoline and catharanthine with respective yields of 3 and 2 mg per 1 g of dried leaves of the plant. the methodology is also applied for identification of the above and other alkaloids from cultured plant cells.19902079546
the discovery of the vinca alkaloids--chemotherapeutic agents against folklore medicine, extracts of the leaves of the subtropical plant catharanthus roseus (l.) g. don (sometimes known as madagascar periwinkle) were reputed to be useful in the treatment of diabetes. this review describes how attempts to verify the antidiabetic properties of the extracts led instead to the discovery and isolation of two complex indole alkaloids, vinblastine and vincristine, which are used in the clinical treatment of a variety of cancers. the two alkaloids, although structurall ...19902085431
characterization of tonoplast-enriched vesicles isolated by gradient density fractionation of suspension-cultured cells.a tonoplast-enriched microsomal fraction was isolated from catharanthus roseus cells. it was characterized by structural and functional criteria. this fraction presented a homogeneous size distribution as shown by quasi-elastic light scattering and a homogeneous density on self-generated gradients of percoll. the mean diameter of the vesicles was estimated to be 0.30 micron and the buoyant density around 1.04 g/ml. sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of its polypep ...19902139317
deduced amino acid sequence of bovine aromatic l-amino acid decarboxylase: homology to other decarboxylases.nucleotide sequence of cdna for bovine aromatic l-amino acid decarboxylase (aadc) was analyzed. the deduced amino acid sequence of bovine aadc shows 57% identity to drosophila aadc and 37% to plant catharanthus roseus aadc. the 7-amino acid sequence of the pyridoxal phosphate binding site is completely conserved among drosophila, pig and bovine aadc. aadc primary structure also shows high homology to that of feline glutamic acid decarboxylase.19902166204
identification of non-equilibrium glycolytic reactions in suspension-cultured plant cells.levels of all enzymes and metabolites involved in glycolysis were determined in suspension-cultured catharanthus roseus cells. from both the maximum catalytic activities of the enzymes and comparisons of mass-action ratios with the apparent equilibrium constants for the reactions, it is concluded that the reactions catalysed by hexokinase, fructokinase, phosphofructokinase and pyruvate kinase are far from equilibrium, whereas the other reactions including that catalysed by pyrophosphate-fructose ...19902223831
a reversible carrier mediates the transport of malate at the tonoplast of catharanthus roseus cells.the conditions of malate transport were defined in tonoplast vesicles purified from a microsomal homogenate of catharanthus roseus cells by preparative free-flow electrophoresis. isolated vesicles exhibited malate transport when the membranes were prepared by grinding the cells in a homogenisation medium only buffered in the acidic ph range. by using vesicles energized artificially by an imposed ph gradient (acid interior), it was shown that malate is actively accumulated in response to the gene ...19902262004
purification and characterization of acetylcoenzyme a: deacetylvindoline 4-o-acetyltransferase from catharanthus roseus.the enzyme acetylcoenzyme a:deacetylvindoline 4-o-acetyltransferase (ec 2.3.1.-) (dat), which catalyzes the final step in vindoline biosynthesis in catharanthus roseus, was purified 3300-fold using ammonium sulfate precipitation followed by gel filtration, anion exchange, hydroxyapatite, and affinity chromatographies. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) of the purified dat showed the presence of two major proteins having mr values of 33,000 and 21,000, whereas na ...19902350183
effect of thermal power plant emissions on catharanthus roseus l. 19902354262
gas chromatographic-mass spectrometric analysis of major indole alkaloids of catharanthus roseus. 19902355069
photochemical one-pot synthesis of vinblastine and vincristine.the naturally occurring cytostatic dimer alkaloids vinblastine 1 and vincristine 2 were photochemically synthesized in slightly acidic aqueous solution from the monomer alkaloids catharanthine 3 and vindoline 4. multi-centre reactions should obviously be involved and some of the principal photochemistry-associated phenomena here discussed are quite likely to be characteristic even to the biosynthetic reactions yielding vinblastine 1 and vincristine 2 in the cells of catharanthus roseus.19902367548
nucleotide sequence of a cdna encoding the vacuolar protein strictosidine synthase from catharanthus roseus. 19902395663
identification of indole alkaloids of catharanthus roseus with liquid chromatography/mass spectrometry using collision-induced dissociation with the thermospray ion repeller.fragmentation of protonated molecular ions produced from catharanthine, tabersonine, ajmalicine and serpentine in a high-performance liquid chromatography (hplc) thermospray ion source was studied. collision-induced dissociation (cid) of the compounds was achieved by merely increasing the repeller potential; i.e. no discharge current was applied and filament was not on. proportion of fragments to the protonated molecular ions increased with the potential at the 180-350 v range studied, but overa ...19902400851
electrochemical detection of indole alkaloids of catharanthus roseus in high-performance liquid chromatography.hydrodynamic voltammograms for indole and five indole alkaloids with different amino functions were obtained in order to evaluate the applicability of high-performance liquid chromatography (hplc) with coulometric detection to these compounds. with the exception of serpentine, which has a quaternary nitrogen in its structure, all the compounds were oxidised and gave net signals of greater than 25 na pmol(-1) at potentials of between +0.2 and +0.85 v versus a solid pd electrode in an acetate-buff ...19892619069
molecular cloning and analysis of cdna encoding a plant tryptophan decarboxylase: comparison with animal dopa decarboxylases.the sequence of a cdna clone that includes the complete coding region of tryptophan decarboxylase (ec, formerly ec from periwinkle (catharanthus roseus) is reported. the cdna clone (1747 base pairs) was isolated by antibody screening of a cdna expression library produced from poly(a)+ rna found in developing seedlings of c. roseus. the clone hybridized to a 1.8-kilobase mrna from developing seedlings and from young leaves of mature plants. the identity of the clone was confirm ...19892704736
assay of strictosidine synthase from plant cell cultures by high-performance liquid hplc assay is described for the enzyme strictosidine synthase in which the formation of strictosidine and the decrease of tryptamine can be followed at the same time. in cell cultures of catharanthus roseus significant amounts of strictosidine glucosidase activity were detected. in crude preparations, the strictosidine synthase reaction is therefore best measured by the secologanin-dependent decrease of tryptamine. in this way, the specific synthase activity in a cell free extract was found t ...19892742131
glycaemic effects of traditional european plant treatments for diabetes. studies in normal and streptozotocin diabetic mice.twelve plants used for the traditional treatment of diabetes mellitus in northern europe were studied using normal and streptozotocin diabetic mice to evaluate effects on glucose homeostasis. the plants were administered in the diet (6.25% by weight) and/or as decoctions or infusions in place of drinking water, to coincide with the traditional method of preparation. treatment for 28 days with preparations of burdock (arctium lappa), cashew (anacardium occidentale), dandelion (taraxacum officinal ...19892743711
adhesion of suspension-cultured catharanthus roseus cells to surfaces: effect of ph, ionic strength, and cation valency.the correlation between the effects of ph, ionic strength and cation valency on the electrophoretic mobility and the extent of adhesion of suspension-cultured catharanthus roseus cells to various polymer substrates is presented. the electrophoretic mobility of cells was unaltered in the ph range of 6-8, but decreased from approximately -2.2 x 10(-8) m v-1 s-1 and approached zero as the ph of the suspending liquid was decreased from 6 to 2. similarly, the value of electrophoretic mobility decreas ...20072765628
modification of catharanthus roseus alkaloids: a lactone derived from 17-deacetylvinblastine.a spirolactone with anti-tumor activity is formed during the reaction of paraformaldehyde with 17-deacetylvinblastine. the compound was identified by nmr techniques. the chemotherapeutic activity was assessed.19892813571
does a proton-pumping atpase exist in the tonoplast?in order to account for the accumulation of metabolites in plant vacuoles, the existence of a proton-pumping atpase has been widely suggested in the literature. the demonstration of such a tonoplast-bound atpase was merely based on the characterization of a nitrate-sensitive microsomal fraction. in some examples, this atpase activity has been evidenced on vacuole preparations obtained under conditions which were criticized by boller. the application of the reverse phase high-performance liquid c ...19862878687
the proton-pumps at the plasmalemma of catharanthus roseus cells.cultured catharanthus roseus cells exhibit transmembrane ferricyanide (fic) reduction which is associated with a proton translocation and a decrease in the atp content of the cells. the h+ efflux and the atp consumption may be counteracted by vanadate, a specific inhibitor of the atpase activity, and by na2wo4 which prevents fic reduction. from these data it is concluded that the redox chain could be coupled with atp hydrolysis for electrogenic proton extrusion which may involve a redox control ...19862878688
auxin induces rapid changes in phosphatidylinositol metabolites.the plant growth-hormone auxin (indole-3-acetic acid, iaa) is involved in regulating such diverse processes as cell elongation, cell division and differentiation. the sequence of events leading to the various phenomena is still poorly understood. both changes in extra- and intracellular ph (refs 1-4) and selective transcription are known to be induced by auxin. evidence for auxin receptors at the plasmalemma membrane has been reported, but the signal transduction pathway is not known, for this n ...19883257546
formation of vinblastine in multiple shoot culture of catharanthus roseus. 19883375331
immunoanalytical methods for screening vindoline from catharanthus roseus cell cultures. 19873444865
elicitor-mediated induction of tryptophan decarboxylase and strictosidine synthase activities in cell suspension cultures of catharanthus roseus.treatment of one cell line (no. 615) of catharanthus roseus c.v. little delicata with an elicitor preparation of autoclaved and homogenized pythium aphanidermatum culture resulted in rapid accumulation of indole alkaloids. alkaloid formation was preceded by rapid transient increases in the extractable activities of the enzymes tryptophan decarboxylase and strictosidine synthase. the induction of these two enzyme activities occurred when cells were transferred to alkaloid production medium or tre ...19873579315
increased synthesis of ajmalicine and catharanthine by cell suspension cultures of catharanthus roseus in response to fungal culture-filtrates.the ammonium sulfate-precipitated fraction from mycelia and culture-filtrates and the crude, cell-free culture filtrates from the growth medium of the fungi chrysosporium palmorum, eurotium rubrum, micromucor isabellina, and pythium aphanidermatum when aseptically added to cell suspensions of cantharanthus roseus caused a rapid and dramatic increase in indole alkaloid biosynthesis. up to 400 micrograms/l ajmalicine and 600 micrograms/l catharanthine were detected in c. roseus cell suspension gro ...19873619437
assay of tryptophan decarboxylase from catharanthus roseus plant cell cultures by high-performance liquid assay is described for the enzyme tryptophan decarboxylase from plant cell suspension cultures. it is based on the fluorometric detection of tryptamine by hplc on a lichrosorb rp-8 select b column. tryptophan decarboxylase from catharanthus roseus was induced by transferring 14-day-old cells into an induction medium. optimum activity was found 2 days after transfer, the increase being 5- to 10-fold. when kept at -15 degrees c the crude enzyme lost half its activity in about 7 days. the rate o ...20133688427
[antitumor and toxic effects of amotin].an indole alkaloid isolated from catharanthus roseus in the soviet union and named amotin differs in terpenoid moiety structure of the indole part of the molecule from the foreign preparation vinblastine and its soviet analog rosevine. in experimental study amotin has exhibited a high antileukemic activity which was more expressed than that of vinblastine. tolerant doses of amotin cause moderate reversible morphological alterations in hematopoietic organs, gastrointestinal mucosa, liver, kidney, ...19873691399
purification and partial characterization of microsomal cytochrome b555 from the higher plant catharanthus roseus.microsomal b-type hemoprotein designated, cytochrome b555 of c.roseus seedlings was solubilized using detergents and purified by a combination of ion exchange chromatography and gel filtration to a specific content of 18.5 nmol per mg of protein. the purified cytochrome b555 was homogeneous and estimated to have an apparent molecular weight of 16500 on sds-page. the absorption spectrum of the reduced form has major peaks at 424, 525 and 555 nm. the alpha-band of the reduced form is asymmetric wi ...20133754748
evidence of phosphorylated phosphatidylinositols in the growth cycle of suspension cultured plant cells.suspension cultured cells of catharanthus roseus rapidly consume the inorganic phosphate of the medium and incorporate about 25% of it into their phospholipids. by three different methods of analysis it was shown that from these plant cells phosphorylated phosphatidylinositol species can be extracted; the mono- and diphosphorylated species were detected in amounts of about 7 and 1%, respectively, of the phosphatidylinositol fraction. autoradiography of 32p-labeled phospholipids showed that espec ...20143947362
a phosphorus-31 nuclear magnetic resonance study of phosphate uptake and storage in cultured catharanthus roseus and daucus carota plant cells.high resolution 31p nmr spectra (103.2 mhz) of oxygenated catharanthus roseus and daucus carota cells grown in suspension cultures were obtained using a solenoidal perfusion probe. the spectra showed resonances for various phosphorylated metabolites such as atp, adp, nad(p)(h), nucleoside diphosphoglucose, and sugar phosphates. the relative levels of the phosphorylated metabolites remained constant throughout the growth curve. no resonances for storage compounds such as polyphosphates, pyrophosp ...19853972837
antifertility efficacy of catharanthus roseus linn: a biochemical and histological study.oral administration of catharanthus roseus linn, leaf extract caused widespread testicular necrosis, hyalinization of tubules and sertoli cell-only-syndrome. biochemical studies revealing notable reduction in glycogen and fructose levels in reproductive tissues support the histological observations and confirm the antifertility properties of c. roseus extract.19854036518
[place of vinca rosea alkaloids (catharanthus roseus) in the treatment of hodgkin's disease]. 19734198540
[alkaloids of catharanthus roseus g. don.--new group of biologically active compounds]. 19684872670
the effect of antibiotics on the growth of catharanthus roseus tissue cultures. 19705495518
medicinal plants--old and new.the historic role of plants in healing declined early in the twentieth century with the ascendency of synthetic drugs, even though a number of basic medical tools, such as opium, strychnine, and cocaine, are of botanical origin. in recent years, interest in natural products has been restored dramatically by the discovery of penicillin, plant-derived tranquilizers, and plant precursors of cortisone. contrary to previous beliefs, botanical drugs are proving more economical than synthetics and hold ...19685644801
effect of gibberellic acid on the growth, alkaloid production, and vlb content of catharanthus roseus. 19685652148
the catharanthus roseus (vinca rosea) alkaloids: a new class of stathmokinetic agents. 19685659730
[alkaloids with tumor inhibiting action from catharanthus roseus]. 19695769032
growth and alkaloid formation in catharanthus roseus tissue cultures. 19695812244
catharosine, a new alkaloid from catharanthus roseus g.don. 19655842019
[the antitumour action and toxicity of the alkaloidal fraction ac-875 from catharanthus roseus]. 19655898964
quantitation of the delivery of liposome contents into plant protoplasts.a procedure for the quantitation of the delivery of liposome contents into catharanthus roseus protoplasts has been developed. the method is based on the uptake of liposome encapsulated methylumbelliferyl beta-d-glucoside and its enzymatic hydrolysis to yield fluorescent methylumbelliferone. since the free glucoside is not taken up by the protoplasts to a significant extent, the delivery of material in the nanomole range can be measured with ease.19846476383
isolation and structural studies on the alkaloids in flowers of catharanthus roseus. 20086481367
catharanthus alkaloids xxxvii. 16-epi-z-isositsirikine, a monomeric indole alkaloid with antineoplastic activity from catharanthus roseus and rhazya stricta.16-epi-z-isositsirikine (1) has been isolated from the leaves of catharanthus roseus and rhazya stricta and identified through a combination of spectral interpretation and chemical correlation. the compound displayed antineoplastic activity in the kb test system in vitro and the p-388 test system in vivo.20086619887
catharanthus alkaloids, xxxviii. confirming structural evidence and antineoplastic activity of the bisindole alkaloids leurosine-n'b-oxide (pleurosine), roseadine and vindolicine from catharanthus roseus.additional and confirming chemical and spectroscopic evidence for vindolicine (4), roseadine (5), and leurosine-n'b-oxide (6) is presented. leurosine-n'b-oxide (6) was found to be exceptionally active in the b-16 melanoma test system in vivo. roseadine (5), a new isolate of catharanthus roseus, and 6 displayed significant activity in the p-388 lymphocytic leukemia test system in vivo. preliminary spectral studies on the new alkaloid roseamine are also described.20086631435
polycationic polypeptides: a possible model for the penetration-enhancing factor in the invasion of host cells by toxoplasma gondii.the effect of polycationic polypeptides (polylysine, polyarginine and polyhistidine) on the invasion of mammalian cells and plant protoplasts by toxoplasma gondii was studied. in jm cells, a human lymphoblastoid cell line with t cell characteristics, all polycationic polypeptides used increased the invasion rate in a concentration-dependent manner. this effect and the morphological changes revealed by electron microscopy resembled the action of the penetration-enhancing factor previously describ ...19846736923
quinone reductases of higher plants.nad(p)h: quinone oxidoreductase (dt-diaphorase) was detected in 100000 x g supernatant fractions of extracts of a wide variety of higher plants. smaller amounts were also found in microsomes and chloroplast fractions. the enzyme was partially purified from soluble extracts of several plants and the quinone reductase from catharanthus roseus was enriched 25-fold. plant quinone reductases have molecular weights in the range of 38000-53000 as determined by gel filtration. the plant enzyme is far le ...19827140760
catharanthus alkaloids. xxxiv. catharanthamine, a new antitumor bisindole alkaloid from catharanthus roseus. 19817264679
catharanthus alkaloids. xxxvi. isolation of vincaleukoblastine (vlb) and periformyline from catharanthus trichophyllus and pericyclivine from catharanthus roseus. 20137264682
catharanthus alkaloids. xxxv. isolation of leurosidine n'b-oxide from catharanthus roseus. 20147320741
catharanthus alkaloids xxxiii. 21'oxo-leurosine from catharanthus roseus (apocynaceae). 20147400819
isolation and characterization of a rice cdna similar to the s-phase-specific cyc07 gene.we isolated a rice cdna clone (t151) which encodes an open reading frame of 262 amino acids. this clone is similar to the s-phase-specific cyc07 gene of catharanthus roseus. expression of this gene is much higher in callus than in seedlings and regulated by external stresses such as high osmotic pressure, salinity, low temperature and submergence.19947510136
plant defense metabolism is increased by the free radical-generating compound aaph.effects of the free radical-generating substance 2,2'-azobis(2-amidinopropane) dihydrochloride (aaph) on defense systems in plant tissue cultures were investigated. exposure of catharanthus roseus, c. tricophyllus, and pisum sativum cultures to aaph caused altered levels of reduced and oxidized glutathione. an increased total glutathione content in c. roseus was prevented by the glutathione biosynthesis inhibitor buthionine-sulfoximine. the specific phenylalanine ammonia-lyase activity in a c. r ...19957557546
cinnamate 4-hydroxylase from catharanthus roseus, and a strategy for the functional expression of plant cytochrome p450 proteins as translational fusions with p450 reductase in escherichia coli.a pcr-based approach was used to isolate cdnas for cinnamate 4-hydroxylase (c4h) from catharanthus roseus cell cultures. the protein shared 75.9% identity with c4h from other plants, and the transcription was induced under various stress conditions. the cloned protein was used to investigate the functional expression of plant p450/p450-reductase fusions in e. coli. fusions containing a modified n-terminal membrane anchor were located in the membrane and possessed c4h activity without solubilizat ...19957589568
vitamin-b12-independent methionine synthase from a higher plant (catharanthus roseus). molecular characterization, regulation, heterologous expression, and enzyme properties.methionine synthases catalyze the formation of methionine by the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine. this reaction is the last step in l-methionine biosynthesis, and it also serves to regenerate the methyl group of s-adenosylmethionine, a cofactor required for biological methylation reactions. we describe the cloning, expression and characterization of a methionine synthase from the higher plant catharanthus roseus. cdnas were identified that encoded a prote ...19957601135
a cdna encoding a plant homologue to animal hmg box proteins involved in structure-specific recognition of dna (ssrp family).we report a cdna encoding a 71-kda protein with a single high-mobility group (hmg) box and two nuclear localization signals from the higher plant catharanthus roseus (madagascar periwinkle). the protein had 40% amino acid identity with animal dna-binding proteins of the ssrp (structure-specific recognition protein) family that recognize bent, unwound dna structures. genomic southern analysis suggested the presence of two genes.19957665097
induction of ajmalicine formation and related enzyme activities in catharanthus roseus cells: effect of inoculum catharanthus roseus cell cultures the time courses of four enzyme activities, tryptophan decarboxylase (tdc), strictosidine synthase (sss), geraniol-10-hydroxylase (g10h) and anthranilate synthase (as), and alkaloid accumulation were compared under two different culture conditions (low-inoculum density and high-inoculum density on induction medium) and a control on growth medium. in growth medium a transient increase in tdc activity was first observed after which g10h reached its maximum acti ...19937763550
large-scale cultivation of catharanthus roseus cells: production of ajmalicine in a 20-l airlift bioreactor.bioreactor systems have been developed for the production of ajmalicine, an alkaloid used in the treatment of hypertension. cell cultures of catharanthus roseus produced higher levels of ajmalicine (323 micrograms g-1 dry weight) in a production medium enriched with tryptophan. the cell cultures were grown in medium prepared in tap water and market sugar with a view to minimise the costs of production. large-scale cultivation of cell suspension was performed in a 20-l airlift bioreactor under co ...19947765026
introduction of oxygenated functional groups into 3-carene and 2-pinene by cultured cells.the biotransformation of the monoterpene hydrocarbons 3-carene and 2-pinene by cell suspension cultures of nicotiana tabacum and catharanthus roseus was investigated. the cultures have the ability to regio- and enantioselectively introduce the oxygenated functional groups into the c = c double bond and the allylic positions of the substrates.19947765622
effects of mercury (ii) species on cell suspension cultures of catharanthus roseus. 19947833617
Displaying items 1 - 100 of 989