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selection and validation of reference genes for transcript normalization in gene expression studies in catharanthus roseus.quantitative real-time pcr (qpcr), a sensitive and commonly used technique for gene expression analysis, requires stably expressed reference genes for normalization of gene expression. up to now, only one reference gene for qpcr analysis, corresponding to 40s ribosomal protein s9 (rps9), was available for the medicinal plant catharanthus roseus, the only source of the commercial anticancer drugs vinblastine and vincristine. here, we screened for additional reference genes for this plant species ...201425058454
analyses of catharanthus roseus and arabidopsis thaliana wrky transcription factors reveal involvement in jasmonate signaling.to combat infection to biotic stress plants elicit the biosynthesis of numerous natural products, many of which are valuable pharmaceutical compounds. jasmonate is a central regulator of defense response to pathogens and accumulation of specialized metabolites. catharanthus roseus produces a large number of terpenoid indole alkaloids (tias) and is an excellent model for understanding the regulation of this class of valuable compounds. recent work illustrates a possible role for the catharanthus ...201424950738
geraniol hydroxylase and hydroxygeraniol oxidase activities of the cyp76 family of cytochrome p450 enzymes and potential for engineering the early steps of the (seco)iridoid pathway.the geraniol-derived (seco)iridoid skeleton is a precursor for a large group of bioactive compounds with diverse therapeutic applications, including the widely used anticancer molecule vinblastine. despite of this economic prospect, the pathway leading to iridoid biosynthesis from geraniol is still unclear. the first geraniol hydroxylation step has been reported to be catalyzed by cytochrome p450 enzymes such as cyp76b6 from catharanthus roseus and cyp76c1 from arabidopsis thaliana. in the prese ...201323933465
comprehensive protein-based artificial microrna screens for effective gene silencing in plants.artificial microrna (amirna) approaches offer a powerful strategy for targeted gene manipulation in any plant species. however, the current unpredictability of amirna efficacy has limited broad application of this promising technology. to address this, we developed epitope-tagged protein-based amirna (etpamir) screens, in which target mrnas encoding epitope-tagged proteins were constitutively or inducibly coexpressed in protoplasts with amirna candidates targeting single or multiple genes. this ...201323645631
divergent dna-binding specificities of a group of ethylene response factor transcription factors involved in plant defense.transcription factors (tfs) recognize target dna sequences with distinct dna-binding domains (dbds). the dbd of arabidopsis (arabidopsis thaliana) ethylene response factor1 (aterf1) uses three consecutive β-strands to recognize a gcc-containing sequence, but tobacco (nicotiana tabacum) erf189 and periwinkle (catharanthus roseus) octadecanoid-derivative responsive catharanthus ap2-domain protein3 (orca3) of the same tf subgroup appear to target similar but divergent dna sequences. here, we examin ...201323629834
biochemical evaluation of the decarboxylation and decarboxylation-deamination activities of plant aromatic amino acid decarboxylases.plant aromatic amino acid decarboxylase (aaad) enzymes are capable of catalyzing either decarboxylation or decarboxylation-deamination on various combinations of aromatic amino acid substrates. these two different activities result in the production of arylalkylamines and the formation of aromatic acetaldehydes, respectively. variations in product formation enable individual enzymes to play different physiological functions. despite these catalytic variations, arylalkylamine and aldehyde synthes ...201323204519
a single gene encodes isopentenyl diphosphate isomerase isoforms targeted to plastids, mitochondria and peroxisomes in catharanthus roseus.isopentenyl diphosphate isomerases (idi) catalyze the interconversion of the two isoprenoid universal c5 units, isopentenyl diphosphate and dimethylally diphosphate, to allow the biosynthesis of the large variety of isoprenoids including both primary and specialized metabolites. this isomerisation is usually performed by two distinct idi isoforms located either in plastids/peroxisomes or mitochondria/peroxisomes as recently established in arabidopsis thaliana mainly accumulating primary isopreno ...201222638903
the jasmonate-responsive element from the orca3 promoter from catharanthus roseus is active in arabidopsis and is controlled by the transcription factor atmyc2.jasmonates are plant signaling molecules that play key roles in protection against certain pathogens and against insects by switching on the expression of genes encoding defense proteins including enzymes involved in the biosynthesis of toxic secondary metabolites. in catharanthus roseus, the ethylene response factor (erf) transcription factor orca3 controls the jasmonate-responsive activation of terpenoid indole alkaloid biosynthetic genes. orca3 gene expression is itself induced by jasmonate. ...201121306988
molecular cloning and characterization of crmdr1, a novel mdr-type abc transporter gene from catharanthus roseus.a novel gene encoding a mdr-like abc transporter protein was cloned from catharanthus roseus, a medicinal plant with more than 120 kinds of secondary metabolites, through rapid amplification of cdna ends (race). this gene (named as crmdr1; genbank accession no.: dq660356) had a total length of 4395 bp with an open reading frame of 3801 bp, and encoded a predicted polypeptide of 1266 amino acids with a molecular weight of 137.1 kda. the crmdr1 protein shared 59.8, 62.5, 60.0 and 58.2% identity wi ...200717541839
an efficient chemoenzymatic production of small molecule glucosides with in situ udp-glucose recycling.a one-pot system for efficient enzymatic synthesis of curcumin glucosides is described. the method couples the activities of two recombinant enzymes, udp-glucose: curcumin glucosyltransferase from catharanthus roseus (caugt2) and sucrose synthase from arabidopsis thaliana (atsus1). udp, a product inhibitor of udp-glucosyltransferase, was removed from the system and used for regeneration of udp-glucose by the second enzyme, atsus1. the productivity was increased several-fold and udp-glucose initi ...200717499718
transcription factor agamous-like 12 from arabidopsis promotes tissue-like organization and alkaloid biosynthesis in catharanthus roseus suspension cells.in catharanthus roseus, monomeric terpenoid indole alkaloids (tias) are biosynthesized in specific tissues, particularly in roots, but failed to be produced by in vitro undifferentiated suspension cells. in this paper, we describe the impact of the root-specific mads-box transcription factor agamous-like 12 (agl12) from arabidopsis thaliana on the differentiation of suspension cells from c. roseus. the expression of agl12 is sufficient to promote an organization of suspension cells into globular ...200717157545
molecular cloning and expression of a cdna encoding a hybrid histidine kinase receptor in tropical periwinkle catharanthus roseus.signalling pathways involving histidine kinase receptors (hkrs) are widely used by prokaryotes and fungi to regulate a large palette of biological processes. in plants, hkrs are known to be implicated in cytokinin, ethylene, and osmosensing transduction pathways. in this work, a full length cdna named crcik was isolated from the tropical species catharanthus roseus (l.) g. don. it encodes a 1205 amino acid protein that belongs to the hybrid hkr family. the deduced amino acid sequence shows the h ...200616773556
differential expression of two type-a response regulators in plants and cell cultures of catharanthus roseus (l.) g. don.two full-length cdnas named crrr2 and crrr3 have been isolated by pcr from a c. roseus cdna library. the first one encodes a 154 amino acid putative protein with a high percentage of identity with the arabidopsis thaliana response regulators arr16 and arr17, and is not expressed in c. roseus organs and cell cultures. the second one encodes a 188 amino acid orf sharing the highest homologies with the a. thaliana arr8 and arr9 response regulators. its expression is root-specific and the transcript ...200312810857
expression analysis in plant and cell suspensions of crckr1, a cdna encoding a histidine kinase receptor homologue in catharanthus roseus (l.) g. don.a full length cdna (crckr1) encoding a hybrid histidine kinase was isolated from a catharanthus roseus cdna library. the kinase belongs to the subfamily of cytokinin receptors represented by cre1/ahk4/wol in arabidopsis thaliana. in cell suspensions, the expression of crckr1 is not affected by various stress and hormonal treatments but is stimulated in cells continuously exposed to cytokinin. in plants, crckr1 is strongly expressed only in the petals of mature flowers. these data suggest that cr ...200212177139
cloning of a cdna encoding an e2 ubiquitin-conjugating enzyme from catharanthus roseus: expression analysis in plant organs and in response to hormones in cell suspensions.a novel cdna (crubie2) encoding ubiquitin-conjugating enzyme e2 was isolated from a catharanthus roseus cdna library. sequence comparison with arabidopsis thaliana e2 sequences revealed that crubie2 is a member of a new plant e2 sub-family. expression of crubie2 is repressed in developing organs and down-regulated by cytokinin suggesting that a decrease in the ubiquitin-dependent proteolytic pathway may take part in the regulation of alkaloid biosynthesis in c. roseus cell suspensions.200211741052
28-norcastasterone is biosynthesized from castasterone.metabolic experiments with deuterium-labeled castasterone in seedlings of arabidopsis thaliana, oryza saliva and lycopersicon esculentum, and cultured cells of catharanthus roseus were performed, and the metabolites were analyzed by gc-ms. in all the plant species examined, [2h3]28-norcastasterone was identified as a metabolite of [26,28-2h6]castasterone, indicating that castasterone is the biosynthetic origin of 28-norcastasterone. moreover, the natural occurrence of 28-norcastasterone and 28-n ...200011065283
brassinosteroids in arabidopsis thaliana.from the seeds and siliques of arabidopsis thaliana, six brassinosteroids, brassinolide, castasterone, typhasterol, 6-deoxocastasterone, 6-deoxotyphasterol and 6-deoxoteasterone, were identified by gc-mass spectrometry or gc-selected ion monitoring. as the occurrence of castasterone, typhasterol, 6-deoxocastasterone and 6-deoxotyphasterol in the shoots of a. thaliana has already been reported, this study provides evidence for the occurrence of the above four brassinosteroids in different organs, ...19989664702
mechanisms of the proliferation and differentiation of plant cells in cell culture systems.plant cell functions have been investigated in various cell culture systems. in this review, we summarize results obtained from investigations of gene expression during the cell cycle in synchronized cultures of catharanthus roseus during somatic embryogenesis in suspension cultures of daucus carota, during organogenesis in tissue cultures of arabidopsis thaliana and during the transdifferentiation of isolated mesophyll cells to tracheary elements in single-cell cultures of zinnia elegans.19947981037
uptake and accumulation of ajmalicine into isolated vacuoles of cultured cells of catharanthus roseus (l.) g. don. and its conversion into serpentine.isolated vacuoles from ajmalicine-producing cell suspensions of catharanthus roseus accumulated the alkaloid ajmalicine. dissipation of the transtonoplast ph gradient with nigericin abolished ajmalicine accumulation, whereas dissipation of the transtonoplast potential with valinomycin had no effect. addition of mg-atp resulted in a higher ajmalicine accumulation. serpentine produced by the cells was largely recovered in isolated vacuoles; in contrast, ajmalicine was lost. ajmalicine was converte ...199124193617
over-expression of hmg-coa reductase and amorpha-4,11-diene synthase genes in artemisia annua l. and its influence on artemisinin content.artemisinin, an endoperoxide sesquiterpene lactone, is a novel antimalarial natural product isolated from artemisia annua l. plants. the low concentrations (0.01-1.1%) of this compound in a. annua l. plants is, however, a major constraint for commercialization of artemisinin-based combination therapies (acts) recommended by who for treating malaria caused by multidrug-resistant p. falciparum sp. in this context, in vivo yield improvement programs were undertaken by us. in the present study, hmg- ...201121655998
uv-b-induced signaling events leading to enhanced-production of catharanthine in catharanthus roseus cell suspension cultures.elicitations are considered to be an important strategy towards improved in vitro production of secondary metabolites. in cell cultures, biotic and abiotic elicitors have effectively stimulated the production of plant secondary metabolites. however, molecular basis of elicitor-signaling cascades leading to increased production of secondary metabolites of plant cell is largely unknown. exposure of catharanthus roseus cell suspension culture to low dose of uv-b irradiation was found to increase th ...200717988378
current status of plant products reported to inhibit sperm.this report reviews research on plant-derived agents that prevent sperm production if taken orally by the male or that incapacitate or kill sperm on contact if used vaginally by the female. it would be of great value to develop fertility inhibitors that are totally selective for reproductive systems and enzymes, and there is a possibility that a plant-derived drug may have this effect. plants that have been studied for their fertility inhibiting effects in the male include: aristolochia indica l ...198212179631
plant-cell bioreactors with simultaneous electropermeabilization and electrophoresis.experimental investigations on using low-level electric currents and voltages to extract, transport, and collect intracellular secondary metabolites from plant cells while maintaining their viabilities were conducted focusing on the production of: (1) ionic betalains, mainly negatively-charged betanin, from beta vulgaris cells, and (2) ionic alkaloids, particularly positively-charged ajmalicine and yohimbine, from catharanthus roseus cells. three versions of tubular membrane reactors in which el ...200312413782
characterization of a cdna encoding a proline-rich 14 kda protein in developing cortical cells of the roots of bean (phaseolus vulgaris) seedlings.a cdna clone, corresponding to mrnas preferentially expressed in the roots of bean (phaseolus vulgaris l.) seedlings, was isolated. this clone contains a 381 bp open reading frame encoding a polypeptide of 13.5 kda, designated pvr5 (phaseolus vulgaris root 5). the amino acid sequence of this clone is rich in proline (13.5%) and leucine (12.7%) and shares significant amino acid sequence homology with root-specific and proline-rich proteins from monocots (maize and rice), and proline-rich proteins ...19968639755
inhibition of anthocyanin formation in seedlings and flowers by the enantiomers of α-aminooxy-β-phenylpropionic acid and their n-benzyloxycarbonyl derivatives.both enantiomers of α-aminooxy-β-phenylpropionic acid (aopp), potent inhibitors of l-phenylalanine ammonia-lyase, and their n-benzyloxycarbonyl (n-boc) derivatives inhibit anthocyanin formation in developing flowers of ipomoea tricolor cav. and catharanthus roseus don. as well as in seedlings of brassica oleracea var. caulo-rapa dc (kohlrabi) and b. oleracea var. capitata l. (red cabbage) with little interference with their normal development. kohlrabi seedlings tolerate up to 0.3 mm l-aopp and ...197924407328
four botanical extracts are toxic to the hispine beetle, brontispa longissima, in laboratory and semi-field trials.the potential of botanical extracts such as celosia argenea l. (caryophyllales: amaranthaceae), ricinus communis l. (malpighiales: euphorbiaceae), mikania micrantha humboldt, bonpland & kunth (astrales: asteraceae), and catharanthus roseus (l.) g. don (gentianales: apocynaceae) for the control of brontispa longissima gestro was evaluated in a bioassay and semi-field trial. dose-response bioassay showed no significant difference in oral-toxicity among the extracts of c. argenea, m. micrantha, and ...201222962939
apocynaceae species with antiproliferative and/or antiplasmodial properties: a review of ten genera.apocynaceae is a large family of tropical trees, shrubs and vines with most species producing white latex. major metabolites of species are triterpenoids, iridoids, alkaloids and cardenolides, which are known for a wide range of biological and pharmacological activities such as cardioprotective, hepatoprotective, neuroprotective, anti-inflammatory, anticancer and antimalarial properties. prompted by their anticancer and antimalarial properties, the current knowledge on ten genera (allamanda, als ...201627417173
a pilot study of antioxidant potential of endophytic fungi from some sudanese medicinal plants.to evaluate the total phenolic content and total antioxidant capacity of ethyl acetate extracts of 21 endophytic fungi isolated from five sudanese medicinal plants: calotropis procera, catharanthus roseus, euphorbia prostrate, vernonia amygdalina and trigonella foenum-graecum.201526433653
medicinal plants cultivated in bapedi traditional healers homegardens, limpopo province, south africa.plants used for medicinal purposes are very common feature in bapedi traditional healer's home-gardens, but information about their diversity and application is not available.201425395716
efficient regeneration and improved sonication-assisted agrobacterium transformation (saat) method for catharanthus roseus.catharanthus roseus is an important medicinal plant known for its pharmacological qualities such as antimicrobial, anticancerous, antifeedant, antisterility, antidiabetic activities. more than 130 bioactive compounds like vinblastine, vindoline and vincristine have been synthesized in this plant. extensive studies have been carried out for optimization regeneration and transformation protocols. most of the protocol described are laborious and time-consuming. due to sophisticated protocol of rege ...201728401464
top-down metabolomic approaches for nitrogen-containing metabolites.streamlining the processes that reveal heteroatom-containing metabolites and their biosynthetic genes is essential in integrated metabolomics studies. these metabolites are especially targeted for their potential pharmaceutical activities. by using a fourier-transform ion cyclotron resonance-mass spectrometry (fticr-ms) instrument, we provide top-down targeted metabolomic analyses using ultrahigh-resolution liquid chromatography-mass spectrometry (lc-ms), high-resolution matrix-assisted laser de ...201728225596
the mirnaome of catharanthus roseus: identification, expression analysis, and potential roles of micrornas in regulation of terpenoid indole alkaloid biosynthesis.micrornas (mirnas) regulate numerous crucial biological processes in plants. however, information is limited on their involvement in the biosynthesis of specialized metabolites in plants, including catharanthus roseus that produces a number of pharmaceutically valuable, bioactive terpenoid indole alkaloids (tias). using small rna-sequencing, we identified 181 conserved and 173 novel mirnas (cro-mirnas) in c. roseus seedlings. genome-wide expression analysis revealed that a set of cro-mirnas are ...201728223695
experimental evidence and in silico identification of tryptophan decarboxylase in citrus genus.plant tryptophan decarboxylase (tdc) converts tryptophan into tryptamine, precursor of indolealkylamine alkaloids. the recent finding of tryptamine metabolites in citrus plants leads to hypothesize the existence of tdc activity in this genus. here, we report for the first time that, in citrus x limon seedlings, deuterium labeled tryptophan is decarboxylated into tryptamine, from which successively deuterated n,n,n-trimethyltryptamine is formed. these results give an evidence of the occurrence of ...201728208655
folivory elicits a strong defense reaction in catharanthus roseus: metabolomic and transcriptomic analyses reveal distinct local and systemic responses.plants deploy distinct secondary metabolisms to cope with environment pressure and to face bio-aggressors notably through the production of biologically active alkaloids. this metabolism-type is particularly elaborated in catharanthus roseus that synthesizes more than a hundred different monoterpene indole alkaloids (mias). while the characterization of their biosynthetic pathway now reaches completion, still little is known about the role of mias during biotic attacks. as a consequence, we deve ...201728094274
an npf transporter exports a central monoterpene indole alkaloid intermediate from the vacuole.plants sequester intermediates of metabolic pathways into different cellular compartments, but the mechanisms by which these molecules are transported remain poorly understood. monoterpene indole alkaloids, a class of specialized metabolites that includes the anticancer agent vincristine, antimalarial quinine and neurotoxin strychnine, are synthesized in several different cellular locations. however, the transporters that control the movement of these biosynthetic intermediates within cellular c ...201728085153
transcriptional regulation and transport of terpenoid indole alkaloid in catharanthus roseus: exploration of new research directions.as one of the model medicinal plants for exploration of biochemical pathways and molecular biological questions on complex metabolic pathways, catharanthus roseus synthesizes more than 100 terpenoid indole alkaloids (tias) used for clinical treatment of various diseases and for new drug discovery. given that extensive studies have revealed the major metabolic pathways and the spatial-temporal biosynthesis of tia in c. roseus plant, little is known about subcellular and inter-cellular trafficking ...201628036025
transcription factors regulating uspa genes in catharanthus roseus.rna-seq of the catharanthus roseus sra database was done in order to detect putative universal stress proteins (usps) and their possible controlling factors. previous analysis indicated the existence and characterization of uspa-like genes. in silico analysis of rna-seq database in several plant tissues revealed the possible functions and regulations of some uspa-like transcripts whose transcription factors (tfs) that might drive their expression were detected. blast indicated the existence of t ...201727890577
industrial case study on alkaloids purification by ph-zone refining centrifugal partition chromatography.the industrial potential of ph-zone refining centrifugal partition chromatography has been evaluated by studying the purification of pharmaceutical ingredients at the pilot scale. for the first time, a scale up methodology based on both column capacity and mass transfer efficiency as invariants was developed. the purification of catharanthine and vindoline from an industrial crude extract of aerial parts of catharanthus roseus, was used as a case of study. toluene/ch3cn/water (4/1/5, v/v/v) was ...201627816224
a differentially regulated ap2/erf transcription factor gene cluster acts downstream of a map kinase cascade to modulate terpenoid indole alkaloid biosynthesis in catharanthus roseus.catharanthus roseus produces bioactive terpenoid indole alkaloids (tias), including the chemotherapeutics, vincristine and vinblastine. transcriptional regulation of tia biosynthesis is not fully understood. the jasmonic acid (ja)-responsive ap2/erf transcription factor (tf), orca3, and its regulator, crmyc2, play key roles in tia biosynthesis. orca3 forms a physical cluster with two uncharacterized ap2/erfs, orca4 and 5. here, we report that (1) the orca gene cluster is differentially regulated ...201727801944
the basic helix-loop-helix transcription factor bis2 is essential for monoterpenoid indole alkaloid production in the medicinal plant catharanthus roseus.monoterpenoid indole alkaloids (mias) are produced as plant defence compounds. in the medicinal plant catharanthus roseus, they comprise the anticancer compounds vinblastine and vincristine. the iridoid (monoterpenoid) pathway forms one of the two branches that feed mia biosynthesis and its activation is regulated by the transcription factor (tf) basic helix-loop-helix (bhlh) iridoid synthesis 1 (bis1). here, we describe the identification and characterisation of bis2, a jasmonate (ja)-responsiv ...201627342401
clade iva basic helix-loop-helix transcription factors form part of a conserved jasmonate signaling circuit for the regulation of bioactive plant terpenoid biosynthesis.plants produce many bioactive, specialized metabolites to defend themselves when facing various stress situations. their biosynthesis is directed by a tightly controlled regulatory circuit that is elicited by phytohormones such as jasmonate (ja). the basic helix-loop-helix (bhlh) transcription factors (tfs) bhlh iridoid synthesis 1 (bis1) and triterpene saponin activating regulator (tsar) 1 and 2, from catharanthus roseus and medicago truncatula, respectively, all belong to clade iva of the bhlh ...201627694525
class ii cytochrome p450 reductase governs the biosynthesis of alkaloids.expansion of the biosynthesis of plant specialized metabolites notably results from the massive recruitment of cytochrome p450s that catalyze multiple types of conversion of biosynthetic intermediates. for catalysis, p450s require a two-electron transfer catalyzed by shared cytochrome p450 oxidoreductases (cprs), making these auxiliary proteins an essential component of specialized metabolism. cpr isoforms usually group into two distinct classes with different proposed roles, namely involvement ...201627688619
a polymorphic (ga/ct)n- ssr influences promoter activity of tryptophan decarboxylase gene in catharanthus roseus l. don.simple sequence repeats (ssrs) of polypurine-polypyrimidine type motifs occur very frequently in the 5' flanks of genes in plants and have recently been implicated to have a role in regulation of gene expression. in this study, 2 accessions of catharanthus roseus having (ct)8 and (ct)21 varying motifs in the 5'utr of tryptophan decarboxylase (tdc) gene, were investigated for its role in regulation of gene expression. extensive tdc gene expression analysis in the 2 accessions was carried out both ...201627623355
conserved roles of crrlk1l receptor-like kinases in cell expansion and reproduction from algae to angiosperms.receptor-like kinases (rlks) are regulators of plant development through allowing cells to sense their extracellular environment. they facilitate detection of local endogenous signals, in addition to external biotic and abiotic stimuli. the catharanthus roseus rlk1-like (crrlk1l) protein kinase subfamily, which contains feronia, plays a central role in regulating fertilization and in cell expansion mechanisms such as cell elongation and tip growth, as well as having indirect links to plant-patho ...201627621737
generous hosts: what makes madagascar periwinkle (catharanthus roseus) the perfect experimental host plant for fastidious bacteria?although much attention has been paid to the metabolism and biosynthesis of monoterpene alkaloids in catharanthus roseus, its value as an experimental host for a variety of agriculturally and economically important phytopathogenic bacteria warrants further study. in the present study, we evaluated the chemical composition of the phloem and xylem saps of c. roseus to infer the nutritional requirements of phloem- and xylem-limited phytopathogens. periwinkle phloem sap consisted of a rich mixture o ...201627620272
differential network analysis reveals evolutionary complexity in secondary metabolism of rauvolfia serpentina over catharanthus roseus.comparative co-expression analysis of multiple species using high-throughput data is an integrative approach to determine the uniformity as well as diversification in biological processes. rauvolfia serpentina and catharanthus roseus, both members of apocyanacae family, are reported to have remedial properties against multiple diseases. despite of sharing upstream of terpenoid indole alkaloid pathway, there is significant diversity in tissue-specific synthesis and accumulation of specialized met ...201627588023
fungal vincristine from eutypella spp - crp14 isolated from catharanthus roseus induces apoptosis in human squamous carcinoma cell line -a431.catharanthus roseus, a medicinal plant, is known to produce secondary metabolites, vincristine and vinblastine, which are terpenoid indole alkaloids. previously we have reported that eutypella spp - crp14 isolated from stem cutting of this plant had shown significant antiproliferative activity when tested in vitro against hela cell line. the present study was conducted to identify the anticancer compound responsible for the anti-proliferative activity of the fungal extract and to evaluate its in ...201627550200
biosynthesis of silver nanoparticles from catharanthus roseus leaf extract and assessing their antioxidant, antimicrobial, and wound-healing activities.biosynthesis of silver nanoparticles (agnps) from catharanthus roseus leaf extract was carried out, and their characterization, as well as antioxidant, antimicrobial, and wound-healing activities were evaluated. color change, uv-vis spectrum, xrd, ftir, and afm assessments supported the biosynthesis and characterization of agnps. the synthesized agnps showed strong in vitro antioxidant and antimicrobial activities against various pathogens. the in vivo assessment of wound healing in agnps-treate ...201627534756
still stable after 11 years: a catharanthus roseus hairy root line maintains inducible expression of anthranilate synthase.hairy root cultures generated using agrobacterium rhizogenes are an extensively investigated system for the overproduction of various secondary metabolite based pharmaceuticals and chemicals. this study demonstrated a transgenic catharanthus roseus hairy root line carrying a feedback-insensitive anthranilate synthase (as) maintained chemical and genetic stability for 11 years. the as gene was originally inserted in the hairy root genome under the control of a glucocorticoid inducible promoter. a ...201727813337
assay of tryptophan decarboxylase from catharanthus roseus plant cell cultures by high-performance liquid chromatography.an assay is described for the enzyme tryptophan decarboxylase from plant cell suspension cultures. it is based on the fluorometric detection of tryptamine by hplc on a lichrosorb rp-8 select b column. tryptophan decarboxylase from catharanthus roseus was induced by transferring 14-day-old cells into an induction medium. optimum activity was found 2 days after transfer, the increase being 5- to 10-fold. when kept at -15 degrees c the crude enzyme lost half its activity in about 7 days. the rate o ...20133688427
receptor-like kinase complexes in plant innate immunity.receptor-like kinases (rlks) are surface localized, transmembrane receptors comprising a large family of well-studied kinases. rlks signal through their transmembrane and juxtamembrane domains with the aid of various interacting partners and downstream components. the n-terminal extracellular domain defines ligand specificity, and rlk families are sub-classed according to this domain. the most studied of these subfamilies include those with (1) leucine-rich repeat (lrr) domains, (2) lysm domains ...201222936944
a reliable protocol for transformation of catharanthus roseus through agrobacterium tumefaciens.proliferation of axillary shoot buds and multiple shoot formation in catharanthus roseus was obtained in 96 % explants on ms medium (3 % sucrose) containing naa + ba. 2,4-d induced callusing in both, the nodal as well as in leaf segments. leaf-derived callus was used for transformation with agrobacterium tumefaciens lba4404/pbi-s1. bacterial cell concentration, duration of co-cultivation and acetosyringone concentration influenced transformation efficiency. under optimal co-cultivation condition ...200923572917
kinetic analysis of phospholipase c from catharanthus roseus transformed roots using different assaysthe properties of phospholipase c (plc) partially purified from catharanthus roseus transformed roots were analyzed using substrate lipids dispersed in phospholipid vesicles, phospholipid-detergent mixed micelles, and phospholipid monolayers spread at an air-water interface. using [(33)p]phosphatidylinositol 4,5-bisphosphate (pip(2)) of high specific radioactivity, plc activity was monitored directly by measuring the loss of radioactivity from monolayers as a result of the release of inositol ph ...199910444091
development of a kinetic metabolic model: application to catharanthus roseus hairy root.a kinetic metabolic model describing catharanthus roseus hairy root growth and nutrition was developed. the metabolic network includes glycolysis, pentose-phosphate pathway, tca cycle and the catabolic reactions leading to cell building blocks such as amino acids, organic acids, organic phosphates, lipids and structural hexoses. the central primary metabolic network was taken at pseudo-steady state and metabolic flux analysis technique allowed reducing from 31 metabolic fluxes to 20 independent ...200616453114
silencing of tryptamine biosynthesis for production of nonnatural alkaloids in plant culture.natural products have long served as both a source and inspiration for pharmaceuticals. modifying the structure of a natural product often improves the biological activity of the compound. metabolic engineering strategies to ferment "unnatural" products have been enormously successful in microbial organisms. however, despite the importance of plant derived natural products, metabolic engineering strategies to yield unnatural products from complex, lengthy plant pathways have not been widely expl ...200919666570
metabolic reprogramming of periwinkle plant culture.we transformed an alkaloid biosynthetic gene with reengineered substrate specificity into catharanthus roseus. the resulting transgenic plant cell culture produced a variety of unnatural alkaloid compounds when cocultured with simple, achiral, commercially available precursors that the reengineered enzyme was designed to accept. this work demonstrates the power of genetic engineering to retailor the structures of complex alkaloid natural products in plant culture.200919151732
development of efficient catharanthus roseus regeneration and transformation system using agrobacterium tumefaciens and hypocotyls as explants.as a valuable medicinal plant, madagascar periwinkle (catharanthus roseus) produces many terpenoid indole alkaloids (tias), such as vindoline, ajamlicine, serpentine, catharanthine, vinblastine and vincristine et al. some of them are important components of drugs treating cancer and hypertension. however, the yields of these tias are low in wild-type plants, and the total chemical synthesis is impractical in large scale due to high-cost and their complicated structures. the recent development of ...201222748182
co-overexpression of geraniol-10-hydroxylase and strictosidine synthase improves anti-cancer drug camptothecin accumulation in ophiorrhiza pumila.camptothecin (cpt) belongs to a group of monoterpenoidindole alkaloids (tias) and its derivatives such as irinothecan and topothecan have been widely used worldwide for the treatment of cancer, giving rise to rapidly increasing market demands. genes from catharanthus roseus encoding strictosidine synthase (str) and geraniol 10-hydroxylase (g10h), were separately and simultaneously introduced into ophiorrhiza pumila hairy roots. overexpression of individual g10h (g lines) significantly improved c ...201525648209
function of ap2/erf transcription factors involved in the regulation of specialized metabolism in ophiorrhiza pumila revealed by transcriptomics and metabolomics.the hairy roots (hr) of ophiorrhiza pumila produce camptothecin (cpt), a monoterpenoid indole alkaloid used as a precursor in the synthesis of chemotherapeutic drugs. o. pumila hr culture is considered as a promising alternative source of cpt, however, the knowledge about the biosynthetic pathway and regulatory mechanism is still limited. in this study, five genes that encode ap2/erf transcription factors, namely operf1-operf5, were isolated from hr of o. pumila. phylogenetic analysis of ap2/erf ...201628018397
silencing the transcriptional repressor, zct1, illustrates the tight regulation of terpenoid indole alkaloid biosynthesis in catharanthus roseus hairy roots.the catharanthus roseus plant is the source of many valuable terpenoid indole alkaloids (tias), including the anticancer compounds vinblastine and vincristine. transcription factors (tfs) are promising metabolic engineering targets due to their ability to regulate multiple biosynthetic pathway genes. to increase tia biosynthesis, we elicited the tia transcriptional activators (orcas and other unidentified tfs) with the plant hormone, methyl jasmonate (mj), while simultaneously silencing the expr ...201627467510
screening and kinetic studies of catharanthine and ajmalicine accumulation and their correlation with growth biomass in catharanthus roseus hairy roots.context catharanthus roseus (l.) g. don (apocynaceae) is still one of the most important sources of terpene indole alkaloids including anticancer and hypertensive drugs as vincristine and vinblastine. these final compounds have complex pathway and many enzymes are involved in their biosynthesis. indeed, ajmalicine and catharanthine are important precursors their increase can lead to enhance levels of molecules of interest. objective this study aims at selecting the highest yield of hairy root li ...201626983347
an protocol for genetic transformation of catharanthus roseus by agrobacterium rhizogenes a4.catharanthus roseus (l.) g. don is a plant species known for its production of a variety of terpenoid indole alkaloids, many of which have pharmacological activities. catharanthine can be chemically coupled to the abundant leaf alkaloid vindoline to form the valuable anticancer drug vinblastine. to study and extract catharanthine and other metabolites from c. roseus, a technique was developed for producing hairy root cultures. in this study, the agrobacterium rhizogenes a4 was induced in the hai ...201222328251
alkaloid production by transformed root cultures of catharanthus roseus.transformed roots of catharanthus roseus were obtained following infection of detached leaves with agrobacterium rhizogenes. roots would not grow in full strength gamborg's b5 medium but would grow satisfactorily if the medium was diluted to one half strength. little alkaloid appeared in the growth medium but root tissue contained a high level and wide variety of alkaloids. ajmalicine, serpentine, vindolinine and catharanthine were prominent components. vinblastine could also be detected by a co ...198824241871
morphogenetic and chemical stability of long-term maintained agrobacterium-mediated transgenic catharanthus roseus plants.transgenic catharanthus roseus plants (transgenic dhawal [dt] and transgenic nirmal [nt]) obtained from the agrobacterium tumefaciens and agrobacterium rhizognenes-mediated transformations, respectively, have been maintained in vitro for 5 years. plants were studied at regular intervals for various parameters such as plant height, leaf size, multiplication rate, alkaloid profile and presence of marker genes. dt plant gradually lost the gus gene expression and it was not detected in the fifth yea ...201525102992
phytohormones in the formation of crown gall tumors.crown gall tumors were initiated in a variety of plant species by infection with agrobacterium tumefaciens strain b6 and the concomitant changes in the tissue levels of phytohormones, mainly indole-3-acetic acid (iaa) and cytokinins, were analyzed. a comparison was made of these hormones with those produced by virulent and avirulent strains of the bacterium in liquid culture and with those of bacteria-free crown gall callus cultures. specific radioimmunoassays were employed for hormone determina ...198124276937
a picrinine n-methyltransferase belongs to a new family of γ-tocopherol-like methyltransferases found in medicinal plants that make biologically active monoterpenoid indole alkaloids.members of the apocynaceae plant family produce a large number of monoterpenoid indole alkaloids (mias) with different substitution patterns that are responsible for their various biological activities. a novel n-methyltransferase involved in the vindoline pathway in catharanthus roseus showing distinct similarity to γ-tocopherol c-methyltransferases was used in a bioinformatic screen of transcriptomes from vinca minor, rauvolfia serpentina, and c. roseus to identify 10 γ-tocopherol-like n-methy ...201626848097
analytical and fluorimetric methods for the characterization of the transmembrane transport of specialized metabolites in plants.the characterization of membrane transport of specialized metabolites is essential to understand their metabolic fluxes and to implement metabolic engineering strategies towards the production of increased levels of these valuable metabolites. here, we describe a set of procedures to isolate tonoplast membranes, to check their purity and functionality, and to characterize their transport properties. transport is assayed directly by hplc analysis and quantification of the metabolites actively acc ...201626843171
application of natural deep eutectic solvents to the extraction of anthocyanins from catharanthus roseus with high extractability and stability replacing conventional organic solvents.natural deep eutectic solvents (nades) have attracted a great deal of attention in recent times as promising green media. they are generally composed of neutral, acidic or basic compounds that form liquids of high viscosity when mixed in certain molar ratio. despite their potential, viscosity and acid or basic nature of some ingredients may affect the extraction capacity and stabilizing ability of the target compounds. to investigate these effects, extraction with a series of nades was employed ...201626822320
understanding crrlk1l function: cell walls and growth control.to develop successfully in an ever-changing environment, it is essential for plants to monitor and control their growth. therefore, cell expansion is carefully regulated to establish correct cell shape and size. in this review, we explore the role of the catharanthus roseus receptor-like kinase (crrlk1l) subfamily as regulators of cell expansion. recently, the downstream signalling events of individual crrlk1l pathways were discovered, implicating known modulators of cell expansion, such as reac ...201626778775
boosting sensitivity in liquid chromatography-fourier transform ion cyclotron resonance-tandem mass spectrometry for product ion analysis of monoterpene indole alkaloids.in metabolomics, the analysis of product ions in tandem mass spectrometry (ms/ms) is noteworthy to chemically assign structural information. however, the development of relevant analytical methods are less advanced. here, we developed a method to boost sensitivity in liquid chromatography-fourier transform ion cyclotron resonance-tandem mass spectrometry analysis (ms/ms boost analysis). to verify the ms/ms boost analysis, both quercetin and uniformly labeled (13)c quercetin were analyzed, reveal ...201526734034
intraspecific differentiation of hancornia speciosa revealed by simple sequence repeat and random amplified polymorphic dna markers.hancornia speciosa, popularly known as mangabeira, is a fruit tree native to the brazilian cerrado that shows great economic potential, due to its multiple uses. intraspecific classification of this species is difficult because it shows high morphological diversity. an early study of the species reported that there are six botanic varieties that differ morphologically mainly in the shapes of their leaves and flowers. except to note the wide morphological variation and economic potential of this ...201526662392
structural determinants of reductive terpene cyclization in iridoid biosynthesis.the carbon skeleton of ecologically and pharmacologically important iridoid monoterpenes is formed in a reductive cyclization reaction unrelated to canonical terpene cyclization. here we report the crystal structure of the recently discovered iridoid cyclase (from catharanthus roseus) bound to a mechanism-inspired inhibitor that illuminates substrate binding and catalytic function of the enzyme. key features that distinguish iridoid synthase from its close homolog progesterone 5β-reductase are h ...201626551396
anti-diabetic potential of catharanthus roseus linn. and its effect on the glucose transport gene (glut-2 and glut-4) in streptozotocin induced diabetic wistar rats.catharanthus roseus is an important ayurvedic medication in traditional medicine. it is potentially used in countries like india, south africa, china and malaysia for the healing of diabetes mellitus. although, the molecular mechanisms behind this effect are yet to be exclusively explored. due to the great antidiabetic and hyperlipidemic potential of c. roseus, we hypothesized that the insulin mimetic effect of ethanolic extract of c. roseus might add to glucose uptake through improvement in the ...201526490765
crbpf1 overexpression alters transcript levels of terpenoid indole alkaloid biosynthetic and regulatory genes.terpenoid indole alkaloid (tia) biosynthesis in catharanthus roseus is a complex and highly regulated process. understanding the biochemistry and regulation of the tia pathway is of particular interest as it may allow the engineering of plants to accumulate higher levels of pharmaceutically important alkaloids. toward this end, we generated a transgenic c. roseus hairy root line that overexpresses the crbpf1 transcriptional activator under the control of a β-estradiol inducible promoter. crbpf1 ...201526483828
precursor feeding studies and molecular characterization of geraniol synthase establish the limiting role of geraniol in monoterpene indole alkaloid biosynthesis in catharanthus roseus leaves.the monoterpene indole alkaloids (mias) are generally derived from strictosidine, which is formed by condensation of the terpene moiety secologanin and the indole moiety tryptamine. there are conflicting reports on the limitation of either terpene or indole moiety in the production of mias in catharanthus roseus cell cultures. formation of geraniol by geraniol synthase (ges) is the first step in secologanin biosynthesis. in this study, feeding of c. roseus leaves with geraniol, but not tryptopha ...201526398791
receptor-like cytoplasmic kinase maris functions downstream of crrlk1l-dependent signaling during tip growth.growing plant cells need to rigorously coordinate external signals with internal processes. for instance, the maintenance of cell wall (cw) integrity requires the coordination of cw sensing with cw remodeling and biosynthesis to avoid growth arrest or integrity loss. despite the involvement of receptor-like kinases (rlks) of the catharanthus roseus rlk1-like (crrlk1l) subfamily and the reactive oxygen species-producing nadph oxidases, it remains largely unknown how this coordination is achieved. ...201526378127
engineering overexpression of orca3 and strictosidine glucosidase in catharanthus roseus hairy roots increases alkaloid production.catharanthus roseus produces many pharmaceutically important terpenoid indole alkaloids (tias) such as vinblastine, vincristine, ajmalicine, and serpentine. past metabolic engineering efforts have pointed to the tight regulation of the tia pathway and to multiple rate-limiting reactions. transcriptional regulator orca3 (octadecanoid responsive catharanthus ap2-domain protein), activated by jasmonic acid, plays a central role in regulating the tia pathway. in this study, overexpressing orca3 unde ...201626351111
rapid and simultaneous determination of five vinca alkaloids in catharanthus roseus and human serum using trilinear component modeling of liquid chromatography-diode array detection data.a novel chemometrics-assisted high performance liquid chromatography method coupled with diode array detector (hplc-dad) was proposed for the simultaneous determination of vincristine (vcr), vinblastine (vlb), vindoline (vdl), catharanthine (cat) and yohimbine (yhb) in catharanthus roseus (c. roseus) and human serum samples. with the second-order advantage of the alternating trilinear decomposition (atld) method, the resolution and rapid determination of five components of interest in complex ma ...201626321366
structural identification of putative usps in catharanthus roseus.nucleotide sequences of the c. roseus sra database were assembled and translated in order to detect putative universal stress proteins (usps). based on the known conserved uspa domain, 24 pfam putative uspa proteins in c. roseus were detected and arranged in six architectures. the uspa-like domain was detected in all architectures, while the protein kinase-like (or pk-like), (tyr)pk-like and/or u-box domains are shown downstream it. three other domains were also shown to coexist with the uspa do ...201526318047
differential induction of meristematic stem cells of catharanthus roseus and their characterization.plant cell culture technology has been introduced for the mass production of the many useful components. a variety of plant-derived compounds is being used in various fields, such as pharmaceuticals, foods, and cosmetics. plant cell cultures are believed to be derived from the dedifferentiation process. in the present study, an undifferentiated cambial meristematic cell (cmcs) of catharanthus is isolated using histological and genetic methods, and compared with dedifferentiation-derived callus ( ...201526298518
characterization of a second secologanin synthase isoform producing both secologanin and secoxyloganin allows enhanced de novo assembly of a catharanthus roseus transcriptome.transcriptome sequencing offers a great resource for the study of non-model plants such as catharanthus roseus, which produces valuable monoterpenoid indole alkaloids (mias) via a complex biosynthetic pathway whose characterization is still undergoing. transcriptome databases dedicated to this plant were recently developed by several consortia to uncover new biosynthetic genes. however, the identification of missing steps in mia biosynthesis based on these large datasets may be limited by the er ...201526285573
binary stress induces an increase in indole alkaloid biosynthesis in catharanthus roseus.catharanthus roseus is an important medicinal plant, which produces a variety of indole alkaloids of significant pharmaceutical relevance. in the present study, we aimed to investigate the potential stress-induced increase of indole alkaloid biosynthesis in c. roseus using proteomic technique. the contents of the detectable alkaloids ajmalicine, vindoline, catharanthine, and strictosidine in c. roseus were significantly increased under binary stress. proteomic analysis revealed that the abundanc ...201526284098
a novel terpenoid indole alkaloid derived from catharanthine via biotransformation by suspension-cultured cells of catharanthus roseus.although catharanthine (1) is well known as a biosynthetic precursor of the anticancer alkaloid, vinblastine, its alternative metabolic pathways are unclear.201526272394
phytochemica: a platform to explore phytochemicals of medicinal plants.plant-derived molecules (pdms) are known to be a rich source of diverse scaffolds that could serve as the basis for rational drug design. structured compilation of phytochemicals from traditional medicinal plants can facilitate prospection for novel pdms and their analogs as therapeutic agents. atropa belladonna, catharanthus roseus, heliotropium indicum, picrorhiza kurroa and podophyllum hexandrum are important himalayan medicinal plants, reported to have immense therapeutic properties against ...201526255307
the bhlh transcription factor bis1 controls the iridoid branch of the monoterpenoid indole alkaloid pathway in catharanthus roseus.plants make specialized bioactive metabolites to defend themselves against attackers. the conserved control mechanisms are based on transcriptional activation of the respective plant species-specific biosynthetic pathways by the phytohormone jasmonate. knowledge of the transcription factors involved, particularly in terpenoid biosynthesis, remains fragmentary. by transcriptome analysis and functional screens in the medicinal plant catharanthus roseus (madagascar periwinkle), the unique source of ...201526080427
isolation of catharanthus roseus (l.) g. don nuclei and measurement of rate of tryptophan decarboxylase gene transcription using nuclear run-on transcription assay.an accurate assessment of transcription 'rate' is often desired to describe the promoter activity. in plants, isolation of transcriptionally active nuclei and their subsequent use in nuclear run-on assays has been challenging and therefore limit an accurate measurement of gene transcription 'rate'. catharanthus roseus has emerged as a model medicinal plant as it exhibits an unsurpassed spectrum of chemodiversity, producing over 130 alkaloids through the terpenoid indole alkaloid (tia) pathway an ...201526024519
biosynthesis and regulation of terpenoid indole alkaloids in catharanthus roseus.catharanthus roseus produces a wide range of terpenoid indole alkaloids (tia). many of them, such as vinblastine and vincristine, have significant bioactivity. they are valuable chemotherapy drugs used in combination with other drugs to treat lymphoma and leukemia. the tia biosynthetic pathway has been investigated for many years, for scientific interest and for their potential in manufacturing applications, to fulfill the market demand. in this review, the progress and perspective of c. roseus ...201726009689
potent antioxidant properties of rolb-transformed catharanthus roseus (l.) g. don.free radicals play an important role in pathological processes such as aging and developing cancer; hence, natural products inhibit free radical production, and can play an important role in preventing diseases. we aimed to evaluate the scavenging activity of free radicals, reducing power, inhibition of lipid peroxidation and malondialdehyde (mda) formation, and the antioxidant composition of rolb-transformed hairy roots and leaf callus of catharanthus roseus. hairy roots of the catharanthus ros ...201627642325
cholinesterase inhibitor (altenuene) from an endophytic fungus alternaria alternata: optimization, purification and characterization.the aim of this study was to screen endophytic fungi isolated from vinca rosea for their potential to produce acetylcholinesterase (ache) inhibitors.201627248836
[endophytic bacterial community analysis of catharanthus roseus and its association with huanglongbing pathogen].to analyze the microbial diversity in healthy and hlb-affected catharanthus roseus under manual-grafting conditions and to find the association between the endophytic bacteria and the hlb pathogen.201222799214
endophytic filamentous fungi from a catharanthus roseus: identification and its hydrolytic enzymes.this paper reported on the various filamentous fungi strains that were isolated from a wild grown catharanthus roseus. based on the morphological characteristics and molecular technique through a polymerase chain reaction and dna sequencing method using internal transcribed spacer (its), these fungi had been identified as a colletotrichum sp., macrophomina phaseolina, nigrospora sphaerica and fusarium solani. the ultrastructures of spores and hyphae were observed under a scanning electron micros ...201627275114
enhanced camptothecin production by ethanol addition in the suspension culture of the endophyte, fusarium solani.ethanolic extract of a non-camptothecin producing plant, catharanthus roseus when added in the suspension culture of the endophyte fusarium solani known to produce camptothecin, resulted in enhanced production of camptothecin by 10.6-fold in comparison to that in control (2.8 μg/l). interestingly, addition of pure ethanol (up to 5% v/v) in the suspension culture of f. solani resulted in maximum enhancement in camptothecin production (up to 15.5-fold) from that obtained in control. in the presenc ...201525603728
useful ethnophytomedicinal recipes of angiosperms used against diabetes in south east asian countries (india, pakistan & sri lanka).this paper is based on data recorded from various literatures pertaining to ethnophytomedicinal recipes used against diabetes in south east asia (india, pakistan and srilanka). traditional plant treatments have been used throughout the world for the therapy of diabetes mellitus. in total 419 useful phytorecipes of 270 plant species belonging to 74 angiospermic families were collected. from the review it was revealed that plants showing hypoglycemic potential mainly belong to the families, cucurb ...201425176368
evidence against the occurrence of adenosine-3':5'-cyclic monophosphate in higher plants.previous reports on the incorporation of [(14)c]adenine into adenosine-3':5'-cyclic monophosphate (cyclic amp) in oat (avena sativa l.) and maize (zea mays l.) coleoptile sections, chick-pea (cicer arietinum l.) embryos and barley (hordeum vulgare l.) aleurone layers were reexamined. separation of labelled nucleotides on deae-sephadex a 25 showed that a peak of (14)c activity, previously considered to be cyclic amp, is not identical with this compound. attempts to detect the cyclic nucleotide by ...197424442328
artemisinic acid serves as a novel orca3 inducer to enhance biosynthesis of terpenoid indole alkaloids in catharanthus roseus cambial meristematic cells.to investigate the effect of artemisinic acid (aa) on improving the production of terpenoid indole alkaloids (tias) of catharanthus roseus cambial meristematic cells (cmcs), feeding aa to c. roseus cmcs caused 2.35-fold and 2.51-fold increases in the production of vindoline and catharanthine, respectively, compared with those of the untreated cmcs. qrt-pcr experiments showed that aa resulted in a 1.36-8.52 fold increase in the transcript levels of several related genes, including octadecanoid-de ...201627534099
structural investigation of heteroyohimbine alkaloid synthesis reveals active site elements that control stereoselectivity.plants produce an enormous array of biologically active metabolites, often with stereochemical variations on the same molecular scaffold. these changes in stereochemistry dramatically impact biological activity. notably, the stereoisomers of the heteroyohimbine alkaloids show diverse pharmacological activities. we reported a medium chain dehydrogenase/reductase (mdr) from catharanthus roseus that catalyses formation of a heteroyohimbine isomer. here we report the discovery of additional heteroyo ...201627418042
the combined effects of ethylene and meja on metabolic profiling of phenolic compounds in catharanthus roseus revealed by metabolomics analysis.phenolic compounds belong to a class of secondary metabolites and are implicated in a wide range of responsive mechanisms in plants triggered by both biotic and abiotic elicitors. in this study, we approached the combinational effects of ethylene and meja (methyl jasmonate) on phenolic compounds profiles and gene expressions in the medicinal plant catharanthus roseus. in virtue of a widely non-targeted metabolomics method, we identified a total of 34 kinds of phenolic compounds in the leaves, co ...201627375495
isolation of cells specialized in anticancer alkaloid metabolism by fluorescence-activated cell sorting.plant specialized metabolism often presents a complex cell-specific compartmentation essential to accomplish the biosynthesis of valuable plant natural products. hence, the disclosure and potential manipulation of such pathways may depend on the capacity to isolate and characterize specific cell types. catharanthus roseus is the source of several medicinal terpenoid indole alkaloids, including the low-level anticancer vinblastine and vincristine, for which the late biosynthetic steps occur in sp ...201627356972
ethylene-induced vinblastine accumulation is related to activated expression of downstream tia pathway genes in catharanthus roseus.we selected different concentrations of ethephon, to stress c. roseus. we used qrt-pcr and hplc followed by pca to obtain comprehensive profiling of the vinblastine biosynthesis in response to ethephon. based on our findings, the results showed that the high concentration of ethephon had a positive effect at both transcriptional and metabolite level. meanwhile, there was a remarkable decrease of hydrogen peroxide content and a promoted peroxidase activity in leaves. the loading plot combination ...201627314017
'candidatus phytoplasma hispanicum', a novel taxon associated with mexican periwinkle virescence disease of catharanthus roseus.mexican periwinkle virescence (mpv) phytoplasma was originally discovered in diseased plants of madagascar periwinkle (catharanthus roseus) in yucatán, mexico. on the basis of results from rflp analysis of pcr-amplified 16s rrna gene sequences, strain mpv was previously classified as the first known member of phytoplasma group 16srxiii, and a new subgroup (16srxiii-a) was established to accommodate mpv phytoplasma. phylogenetic analysis of 16s rrna gene sequences indicated that strain mpv repres ...201627266266
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