| [metabolism of nicotinic acid in plant cell suspension cultures, iv: occurrence and metabolism of nicotinic acid n-alpha-arabinoside (author's transl)]. | application of nicotinic acid to cell suspension cultures of petroselinum hortense hoffm., daucus carota, nicotiana tabacum and nicotiana glauca leads to the formation of the recently isolated[2] nicotinic acid n-alpha-l-arabinoside. in these cell cultures the arabinoside is a metabolically active compound; the nicotinic acid moiety is used for nad synthesis and nicotinic acid degradation involving decarboxylation and ring fission. n-methylnicotinic acid (trigonelline) and nicotinic acid n-alpha ... | 1976 | 185135 |
| repression of asexual embryogenesis in vitro by some plant growth regulators. | a factor that represses asexual embryogenesis has been observed in the rutaceae, with particularly high concentrations in the naturally monoembryonic cultivars. this investigation was an initial step towards identifying the factor. citrus reticulata blanco ponkan mandarin nucellus explants and daucus carota l. 'queen anne's lace' callus were employed to examine effects of known plant growth regulators and to determine possible identity of one or more of them with the repressive factor. the chala ... | 1977 | 145403 |
| isolation and genetic analysis of an agrobacterium tumefaciens avirulent mutant with a chromosomal mutation produced by transposon mutagenesis. | a transposon 5 (tn5) insertion was introduced into the genome of a. tumefaciens (a-208 strain harboring a nopaline type ti-plasmid) using a conjugative pjb4ji plasmid containing tn5. five thousand transconjugants were assayed for virulence on carrot (daucus carota l.) disks; 54 isolates were avirulent or very attenuated. the cellular localization (plasmid or chromosome) of the tn5 insertion in those isolates were identified by southern hybridization analysis. an avirulent mutant (b-90 strain) wi ... | 1992 | 1369092 |
| [production of xanthan gum in immobilized cultures of xanthomonas campestris]. | the efficiency of xanthan production through surface processes was evaluated. the best porous material was selected first. thereafter, a comparative study was performed using submerged agitated process vs other without agitation but containing the selected porous material. the culture medium used was white potatoes infusion, buffered with k2hpo4 and supplemented with glucose in diverse concentrations. besides, to evaluate a different type of surface process, three vegetables were valued: ipomaea ... | 1992 | 1298018 |
| pharmacological studies on daucus carota part i. | | 1975 | 1178783 |
| spatial and temporal expression patterns directed by the agrobacterium tumefaciens t-dna gene 5 promoter during somatic embryogenesis in carrot. | we have analysed the patterns of expression of a gene encoding beta-glucuronidase (gus) fused to the promoter of the agrobacterium tumefaciens t-dna gene 5 during embryogenesis in carrot, daucus carota l. gene expression was monitored by a histochemical assay of beta-glucuronidase activity. the gene 5 promoter, although of bacterial origin, conferred expression upon the marker gene in all stages of embryo development. the patterns of expression however, differed between embryos in different stag ... | 1992 | 1558941 |
| antispasmodic activity of the tertiary base of daucus carota, linn. seeds. | a nitrogen containing base responding to mayer's test and dragendorff's reagent for tertiary bases has been isolated from the seeds of daucus carota linn. the effects of the base as its bromide have been studied on smooth muscles of ileum, uterus, blood vessels and trachea of different species of animals. the tertiary base has been found to have papaverine like nonspecific smooth muscle relaxant and spasmolytic activity, but its activity was found to be about one-tenth of that of papaverine. | 1979 | 521127 |
| isolation and partial characterization of a substance from carrots, daucus carota, with ability to agglutinate cells of streptococcus mutans. | | 1982 | 6184165 |
| studies on daucus carota linn. ii. cholinergic activity of the quaternary base isolated from water-soluble fraction of alcoholic extract of seeds. | | 1966 | 5979224 |
| monoamine oxidase inhibiting activity of daucus carota. | | 1973 | 4783391 |
| studies on the introduction and mobility of the maize activator element in arabidopsis thaliana and daucus carota. | we have co-transformed carrot (daucus carota) and arabidopsis thaliana with an agrobacterium tumefaciens non-tumorigenic t-dna carrying the maize transposable element activator (ac) and an agrobacterium rhizogenes ri t-dna. we present evidence that the ac element transposes in transformed root or root-derived callus cultures of both species. we show that fertile plants can be regenerated from transformed, root-derived callus cultures of arabidopsis, demonstrating the utility of the ri plasmid fo ... | 1987 | 2832144 |
| effect of chromatographic fractions of daucus carota linn. (seeds) on fertility in female albino rats. | | 1972 | 5078109 |
| studies on daucus carota, linn. i. pharmacological studies with the water-soluble fraction of the alcoholic extract of the seeds: a preliminary report. | | 1966 | 5934261 |
| measurement of the five enzymes which convert chorismate to tryptophan in cultured daucus carota cell extracts. | | 1973 | 4750744 |
| tryptophan biosynthesis in nicotiana tabacum and daucus carota cell cultures: site of action of inhibitory tryptophan analogs. | | 1972 | 4536831 |
| anthiovulatory activity of five indigenous plants in rabbits. | the petroleum ether, alcoholic, and aqueous extracts of 5 indigenous plants, known to have antifertility activity in female rats and mice (areca catechu linn, carica papaya linn, daucus carota linn, mentha arvensis linn and polygonum hydropiper linn), were evaluated for their possible antiovulatory activity in rabbits with copper-induced ovulation. the alcoholic extract of mentha arvensis (leaves) and the petroleum ether extract of the roots of polygonum hydropiper were the most successful age ... | 1974 | 4442983 |
| structure and function of the promoter of the carrot v-type h(+)-atpase catalytic subunit gene. | we investigated the 5'-upstream region of the gene encoding the catalytic subunit of the v-type h(+)-atpase in daucus carota. a genomic sublibrary was screened with a cdna probe, and a 4-kilobase genomic clone was obtained covering the first two exons and about 3 kilobases of the 5'-upstream sequence. the intron/exon boundaries matched established consensus sequences. within 240 base pairs (bp) upstream of the initiation codon three putative tata boxes were found. ribonuclease protection and pri ... | 1990 | 2139875 |
| chalcone synthase from cell suspension cultures of daucus carota l. | chalcone synthase (chs) has been partially purified about 35-fold. withdrawal of 2-mercaptoethanol after precipitation with ammonium sulfate led to higher stability during further purification steps. in order to determine chs activity, two procedures [according to schröder et al. (1979) plant sci. lett. 14, 281-286] were applied. the radioactivity extracted with ethyl acetate from the assay mixture (total products) was compared to 14c-labeled flavanone purified by tlc. the activity of chs increa ... | 1985 | 4015104 |
| detection and prevalence of listeria monocytogenes in the agricultural ecosystem. | the sensitivity of four different enrichment procedures to detect listeria monocytogenes in the presence of high levels of streptococcus faecalis was investigated. defined mixed cultures of strep. faecalis and l. monocytogenes gave better results with one-stage enrichment techniques. for manure samples, however, two-stage enrichment techniques gave the best performance. the so-called cold enrichment techniques were found to be unsatisfactory for samples from natural environments. the following m ... | 1991 | 1955415 |
| growth effects, uptake and metabolism of trifluralin in tissue cultures. | growth effects, uptake and metabolism of trifluralin (alpha, alpha, alpha-trifluoro-2,6-dinitro-n,n-dipropyl-p-toluidine) in carrot (daucus carota l.) and tobacco (nicotiana tabaccum l.) callus tissue were determined. carrot callus was initiated from tap root tissue on murashige and skoog medium with kinetin and 2,4-dichlorophenoxy acetic acid (2,4-d). tobacco callus was initiated from pith tissue on murashige and skoog medium with indole-3-acetic acid (iaa) and kinetin. | 1989 | 2809078 |
| characterization of three agrobacterium tumefaciens avirulent mutants with chromosomal mutations that affect induction of vir genes. | three agrobacterium tumefaciens mutants with chromosomal mutations that affect bacterial virulence were isolated by transposon mutagenesis. two of the mutants were avirulent on all hosts tested. the third mutant, ivr-211, was a host range mutant which was avirulent on bryophyllum diagremontiana, nicotiana tabacum, n. debneyi, n. glauca, and daucus carota but was virulent on zinnia elegans and lycopersicon esculentum (tomato). that the mutant phenotype was due to the transposon insertion was dete ... | 1991 | 1846853 |
| molecular cloning, induction and taxonomic distribution of caffeoyl-coa 3-o-methyltransferase, an enzyme involved in disease resistance. | trans-caffeoyl-coa 3-o-methyltransferase is involved in the reinforcement of the plant cell wall under conditions that trigger the disease resistance response (pakusch, a.-e., kneusel, r.e., and matern, u. (1989) arch. biochem. biophys. 271, 488-494). partial amino acid sequences of the enzyme from cultured parsley cells that had been treated with a crude elicitor were identified (pakusch, a.-e., matern, u., and schiltz, e. (1991) plant physiol. 95, 137-143), and corresponding degenerated oligon ... | 1991 | 1894629 |
| purification and characterization of cytosolic aldolase from carrot storage root. | a single fructose-1,6-bisphosphate (fbp) aldolase has been detected in extracts from carrot storage roots (daucus carota l.). the enzyme was purified 850-fold to electrophoretic homogeneity and a final specific activity of 26.3 mumols of fbp utilized/min per mg of protein. sds/page of the final preparation revealed a single protein-staining band of 40 kda. the native molecular mass was determined by analytical gel filtration to be 159 kda, indicating that the enzyme is a homotetramer. denaturing ... | 1990 | 2198022 |
| proteolytic and partial sequencing studies of the bifunctional dihydrofolate reductase-thymidylate synthase from daucus carota. | the bifunctional dihydrofolate reductase-thymidylate synthase (dhfr-ts) of daucus carota has been further characterized as regards molecular weight, amino acid composition, protease digestion and microsequencing of proteolytic peptides. data reported in this paper demonstrate that the carrot protein has a calculated mr of 124,000 thus indicating that, contrarily to what has previously been suggested, it occurs as a dimer of identical subunits. results of partial amino acid microsequencing show t ... | 1991 | 1863769 |
| structure of t-dna in roots transformed by agrobacterium rhizogenes. | dna isolated from hairy roots incited on daucus carota by agrobacterium strains harboring the ri plasmid was probed with ri plasmid fragments to identify plasmid sequences transferred and integrated into the plant genome. one hairy root line was incited by an a. rhizogenes natural isolate strain harboring two large plasmids in addition to the ri plasmid. dna from hairy roots incited by this strain contained a total of five nonidentical copies of t-dna which together comprised a t-dna complement ... | 1983 | 6875427 |
| cloning, nucleotide sequence and expression of the bifunctional dihydrofolate reductase-thymidylate synthase from glycine max. | cdnas encoding the bifunctional dihydrofolate reductase-thymidylate synthase from glycine max were isolated and sequenced. the 1794 base full length cdna contains a single open reading frame of 1593 bases. the predicted size of the encoded protein is 530 amino acids with a molecular weight of 59,707. the protein has two domains: a 226 residue dhfr domain in the n-terminus, which is over 30% identical to human dhfr or the dhfr domain of protozoal dhfr-ts, and a 304 residue thymidylate synthase (t ... | 1995 | 7742362 |
| cloning of a carrot cdna for a member of the family of adp-ribosylation factors (arfs) and characterization of the binding of nucleotides by its product after expression in e. coli. | a cdna clone, designated dcarf1, was isolated from a cdna library prepared from embryogenic cell clusters of daucus carota and characterized. the cdna (883 bp) contained an open reading frame that encoded a protein of 181 amino acid residues with significant homology to the adp-ribosylation factors (arfs) of animals and yeast. the dcarf1-related transcripts were detected at a nearly constant level during somatic embryogenesis. the dcarf1-encoded protein was expressed as a fusion protein in e. co ... | 1995 | 7551586 |
| a trna gene mapping within the chloroplast rdna cluster is differentially expressed during the development of daucus carota. | in vivo analysis of expression of the chloroplast rdna cluster during somatic embryogenesis of daucus carota (d.carota) was performed by northern-blot analysis with different dna probes, spanning both the 16s rrna gene, the 16s-23s rrna spacer, which contains the two mosaic trna genes trna(ile) and trna(ala), and the region upstream of the 16s rrna gene, where a trna(val) maps. we show that expression both of the spacer trnas trna(ile) and trna(ala) is not significantly regulated during developm ... | 1994 | 8202376 |
| active polypeptide fragments common to prokaryotic, eukaryotic, and mitochondrial dna polymerases. | with a procedure that allows the renaturation of the dna polymerase catalytic activity in situ after sds-polyacrylamide gel electrophoresis, we have compared the active polypeptides present in extracts from organisms covering a wide evolutionary range from prokaryotes to eukaryotes, namely: escherichia coli, oryza sativa, daucus carota , neurospora crassa, dictyostelium discoideum, saccharomyces cerevisiae, ceratitis capitata, leucophaea maderae , xenopus laevis, rat tissues and human lymphoblas ... | 1982 | 6765191 |
| research on plants for fertility regulation in india. | this present review of indian plants investigated for fertility regulation includes published literature of the country and unpublished data of the central drug research institute (cdri), located in lucknow, india. publications without supportive experimental data have not been included. it is evident from the data presented in the tables that most of the investigators have failed to include the valuable information on the time and place of collection and proper botanical authentication, if co ... | 1982 | 6752588 |
| isolation and characterization of a cdna clone for plant nuclear antigen 21d7 associated with cell division. | a cdna clone was isolated from a carrot (daucus carota l.) cdna expression library using monoclonal antibody 21d7, which recognizes a nuclear antigen associated with cell division in plant cells. to show that the isolated cdna encodes the 21d7 antigen, a polyclonal antiserum was raised against a recombinant fusion protein specified by the cdna. both the polyclonal antiserum and the monoclonal antibody 21d7 recognized the same plant protein on immunoblots, in immunoprecipitation experiments, and ... | 1993 | 8310059 |
| hepatoprotective activity of carrot (daucus carota l.) against carbon tetrachloride intoxication in mouse liver. | the effect of carrot extract on carbon tetrachloride (ccl4)-induced acute liver damage was evaluated. the increased serum enzyme levels (viz., glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, lactate dehydrogenase, alkaline phosphatase, sorbitol and glutamate dehydrogenase) by ccl4-induction were significantly lowered due to pretreatment with the extract. the extract also decreased the elevated serum bilirubin and urea content due to ccl4 administration. increased activities ... | 1995 | 7500638 |
| isolation and characterization of a new chromosomal virulence gene of agrobacterium tumefaciens. | a mutant (strain b119) of agrobacterium tumefaciens with a chromosomal mutation was isolated by transposon (tn5) mutagenesis. the mutant exhibited growth rates on l agar and minimal medium (ab) plates similar to those of the parent strain (strain a208 harboring a nopaline-type ti plasmid). the mutant was avirulent on all host plants tested: daucus carota, cucumis sativus, and kalanchoe diagremontiana. the mutant was not impaired in attachment ability to carrot cells. the mutant had one insertion ... | 1993 | 8491736 |
| molecular genetics of the maize (zea mays l.) aspartate kinase-homoserine dehydrogenase gene family. | aspartate kinase (ak) and homoserine dehydrogenase (hsdh) are enzymes in the aspartate-derived amino acid biosynthetic pathway. recent biochemical evidence indicates that an ak-hsdh bifunctional enzyme exists in maize (zea mays l.). in this report, we characterize three genes that encode subunits of ak-hsdh. two cdnas, pakhsdh1 and pakhsdh2, containing the full-coding sequence, and one partial cdna, pakhsdh3, encode amino acid sequences similar to the reported monofunctional ak and hsdh enzymes ... | 1994 | 7846152 |
| [granulated carrots (daucus carota) in dog nutrition]. | in feeding and balance trials with dogs (4) dried, ground carrots were tested in addition (30%) to a basic dry food (rice, corn gluten, fish meal and supplements). the palatability of both mixtures was good: fecal water losses were increased after feeding the carrots. apparent digestibilities of organic and inorganic matter, especially sodium and potassium (not phosphorus), decreased. digestibility of carrots was calculated to reach 70% with 1.2 mj of digestible energy per 100 g dry matter. redu ... | 1993 | 8430292 |
| [the in vitro action of plants on vibrio cholerae]. | natural products of several plants, according to the geographic location, are used by peruvian people in the popular treatment of diarrhea, with good success. when cholerae cases appeared in peru, we were interested to know the "in vitro" effect against vibrio cholerae 01, of these useful plants to treat diarrhea. the following plants were tested: cichorium intybus, althaea officinalis, psorela glandulosa, geranium maculatum, punica granatum, malus sativa, cydonia oblonga, chenopodium ambrosoide ... | 1994 | 8018898 |
| phosphoglycerylethanolamine posttranslational modification of plant eukaryotic elongation factor 1alpha. | eukaryotic elongation factor 1alpha (eef-1a) is a multifunctional protein. there are three known posttranslational modifications of eef-1a that could potentially affect its function. except for phosphorylation, the other posttranslational modifications have not been demonstrated in plants. using matrix-assisted laser desorption/ionization-mass spectrometry and peptide mass mapping, we show that carrot (daucus carota l.) eef-1a contains a phosphoglycerylethanolamine (pge) posttranslational modifi ... | 1998 | 9662537 |
| multiple mutations in the transferred regions of the agrobacterium rhizogenes root-inducing plasmids. | agrobacterium rhizogenes induces root formation at the site of inoculation in plants and inserts fragments of its ri plasmid into the plant nuclear dna. the transferred region (t-dna) of the ri plasmid of the a. rhizogenes strain a4 is made of two fragments, namely tl and tr; the latter harbors a sequence homology with the tms loci (responsible for auxin synthesis) of a. tumefaciens. on daucus carota slices, single insertion mutations on the tl region of a. rhizogenes do not confer a mutant phen ... | 1986 | 3012616 |
| expression of dc8 is associated with, but not dependent on embryogenesis. | dc8 is a late embryogenesis-abundant (lea) protein gene isolated from carrot (daucus carota). deletion analysis of the dc8 promoter was performed to determine the sequences required for aba and seed-specific regulation of dc8 transcription. to investigate the mechanism of dc8 expression during seed development, chimeric gene constructs containing dc8 promoter fragments fused to a promoterless beta-glucuronidase gene (dc8:gus) were introduced into carrot, tobacco (nicotiana tobacum) and arabidops ... | 1996 | 8704145 |
| peptide splicing in the vacuolar atpase subunit a from candida tropicalis. | subunit a of the vacuolar proton pump appears to be responsible for the atp hydrolysis which is coupled to the pumping of protons into a variety of intracellular acid compartments, including the fungal vacuole. we report here the cloning and sequence determination of the gene encoding subunit a from candida tropicalis. southern blot hybridization analysis indicates that there is a single gene which encodes this protein. the gene contains a single intron at the extreme 5'-end of the coding region ... | 1993 | 8463270 |
| induced plant responses to pathogen attack. analysis and heterologous expression of the key enzyme in the biosynthesis of phytoalexins in soybean (glycine max l. merr. cv. harosoy 63). | in soybean (glycine max l.), pathogen attack induces the formation of glyceollin-type phytoalexins. the biosynthetic key enzyme is a reductase which synthesizes 4,2', 4'-trihydroxychalcone in co-action with chalcone synthase. screening of a soybean cdna library from elicitor-induced rna in lambda gt11 yielded two classes of reductase-specific clones. the deduced proteins match to 100% and 95%, respectively, with 229 amino acids sequenced in the purified plant protein. four clones of class a were ... | 1991 | 1840523 |
| identification and expression of a cdna from daucus carota encoding a bifunctional aspartokinase-homoserine dehydrogenase. | aspartokinase (ec 2.7.2.4) and homoserine dehydrogenase (ec 1.1.1.3) catalyze steps in the pathway for the synthesis of lysine, threonine, and methionine from aspartate. homoserine dehydrogenase was purified from carrot (daucus carota l.) cell cultures and portions of it were subjected to amino acid sequencing. oligonucleotides deduced from the amino acid sequences were used as primers in a polymerase chain reaction to amplify a dna fragment using dna derived from carrot cell culture mrna as tem ... | 1993 | 8507831 |
| nucleotide sequence of the atpase a- and b-subunits of the halophilic archaebacterium haloferax volcanii and characterization of the enzyme. | using pcr with degenerate oligonucleotides, we amplified two conserved regions of the plasma membrane atpase a (alpha)- and b (beta)-subunit-encoding genes from haloferax volcanii, a halophilic archaeon. the amplified fragments were cloned and sequenced, and then used as homologous probes to clone genomic restriction fragments, one of which contained the nearly complete atpa- and atpb-gene. to complete the latter one, we sequenced a region of an overlapping fragment using synthetic oligonucleoti ... | 1995 | 7599166 |
| orf250 encodes a second subunit of an abc-type heme transporter in oenothera mitochondria. | a highly transcribed region in oenothera mitochondria codes for an open reading frame comprising 250 condons (orf250). this open reading frame shows high sequence similarity to the helc gene of rhodobacter capsulatus which encodes a subunit of a proposed abc-type heme transporter. transcripts of orf250 are edited by cytidine to uridine transitions at 29 sites, altering 10% of all encoded amino acids. genes homologous to helc have also been found in the bacteria bradyrhizobium japonicum and esche ... | 1995 | 7862087 |
| two isoforms of plant rad23 complement a uv-sensitive rad23 mutant in yeast. | dna repair by nucleotide excision (ner) has been demonstrated in plant cells at the biochemical level but until now none of the molecular components of the plant ner complex has been identified. in this paper, the cloning and characterization of two isoforms of rad23 from carrot (daucus carota l.) are reported. it has been suggested that rad23 in yeast is an assembly factor of the ner complex required for transcription-coupled repair as well as efficient overall genome repair. a functional assay ... | 1998 | 9681019 |
| cdna cloning of carrot (daucus carota) soluble acid beta-fructofuranosidases and comparison with the cell wall isoenzyme. | carrot (daucus carota), like most other plants, contains various isoenzymes of acid beta-fructofuranosidase (beta f) (invertase), which either accumulate as soluble polypeptides in the vacuole (isoenzymes i and ii) or are ionically bound to the cell wall (extracellular beta f). using antibodies against isoenzyme i of carrot soluble beta f, we isolated several cdna clones encoding polypeptides with sequences characteristic of beta fs, from bacteria, yeast, and plants. the cdna-derived polypeptide ... | 1994 | 8016265 |
| the highly edited orf206 in oenothera mitochondria may encode a component of a heme transporter involved in cytochrome c biogenesis. | a highly transcribed region in oenothera mitochondria codes for a reading frame (orf206) which shows high homology to the marchantia encoded mitochondrial open reading frame orf277 and is also conserved in the mitochondrial genomes of arabidopsis thaliana and daucus carota. transcripts of orf206 are modified by cytidine to uridine changes in 46 positions by rna editing, affecting 30% of all cytidines and 15% of the total encoded amino acids. this orf is cotranscribed with an upstream reading fra ... | 1994 | 8003696 |
| regulation of enzymes involved in anthocyanin biosynthesis in carrot cell cultures in response to treatment with ultraviolet light and fungal elicitors. | the accumulation of anthocyanins in cell cultures of daucus carota l. and the enzymes involved in their biosynthesis were investigated under growth in the dark, continuous irradiation with uv light, incubation with elicitors from pythium aphanidermatum, and elicitor treatment of uv-irradiated cells. upon uv irradiation, anthocyanin accumulation was strongly enhanced, and the enzymes of the phenylpropanoid and flavonoid pathways, including the "late" enzymes cyanidin galactosyltransferase, cyanid ... | 1998 | 9684371 |
| natural genetic transformation by agrobacterium rhizogenes . annual flowering in two biennials, belgian endive and carrot | genetic transformation of belgian endive (cichorium intybus) and carrot (daucus carota) by agrobacterium rhizogenes resulted in a transformed phenotype, including annual flowering. back-crossing of transformed (r1) endive plants produced a line that retained annual flowering in the absence of the other traits associated with a. rhizogenes transformation. annualism was correlated with the segregation of a truncated transferred dna (t-dna) insertion. during vegetative growth, carbohydrate reserves ... | 1998 | 9765539 |
| elongation factor-1alpha stabilizes microtubules in a calcium/calmodulin-dependent manner. | elongation factor-1alpha (ef-1alpha), a highly conserved protein named for its role in protein translation, is also a microtubule-associated protein (map). we used high-resolution differential interference contrast microscopy to quantify the effect of substoichiometric amounts of ef-1alpha (isolated from daucus carota) on the dynamic instability of microtubules assembled in vitro from either animal or plant tubulin. ef-1alpha modulates the dynamic behavior of microtubules assembled from either t ... | 1998 | 9786091 |
| [chemical, chemosensory and human-sensory experiments on taste and flavor of carrots]. | the relationship between sensory quality of carrots and their contents and composition of essential oils and total sugars as influenced by nitrogen fertilization was investigated. carrots (daucus carota l.) of the variety 'nanthya' f1 (s&g sandoz seeds) were grown in weihenstephan 1996 with three levels of inorganic nitrogen fertilization (3 levels in 4 replications). medium- and higher-boiling flavour-components were extracted as essential oils and separated gas-chromatographically (gc-fid). lo ... | 1998 | 9881369 |
| molecular cloning and analysis of a cdna coding for the bifunctional dihydrofolate reductase-thymidylate synthase of daucus carota. | molecular cloning of dihydrofolate reductase-thymidylate synthase (dhfr-ts) of daucus carota was achieved by immunoscreening of a cdna library obtaining a 2 kbp clone which contains an open reading frame of 1528 bp. comparison of the deduced amino acid sequence with those from other sources revealed the presence of motifs typical of dhfr and ts thus confirming the bifunctional nature of the carrot protein. as in other organisms, a higher degree of conservation was observed in the ts domain. anal ... | 1993 | 8329682 |
| molecular characterization of dau c 1, the bet v 1 homologous protein from carrot and its cross-reactivity with bet v 1 and api g 1. | up to 70% of patients with birch pollen allergy exhibit the so-called oral allergy syndrome, an ige-mediated food allergy. the most frequent and therefore best characterized pollen-fruit syndrome is apple allergy in patients suffering from tree pollen-induced pollinosis. the occurrence of adverse reactions to proteins present in vegetables such as celery and carrots in patients suffering from pollen allergy has also been reported. cdnas for bet v 1 homologous proteins have been cloned from celer ... | 1999 | 10336602 |
| production of glomus intraradices propagules, an arbuscular mycorrhizal fungus, in an airlift bioreactor | this work addresses the symbiotic culture of the arbuscular mycorrhizal (am) fungus glomus intraradices with daucus carota hairy roots transformed by agrobacterium rhizogenes, in two submerged culture systems: petri dish and airlift bioreactor. am fungi play an active role in plant nutrition and protection against plant pathogens. these fungi are obligate biotrophs as they depend on a host plant for their needs in carbohydrates. the effect of the mycorrhizal roots inoculum-to-medium volume ratio ... | 1999 | 10099599 |
| an extracellular insoluble inhibitor of cysteine proteinases in cell cultures and seeds of carrot. | an 18 kda extracellular insoluble protein (eip18) was found previously in amorphous particles suspended in the culture medium and in the interspaces of cell clusters of carrot (daucus carota l.) callus, as well as in the extracellular spaces of carrot seeds, being located both in the embryo and at the inner edge of the endosperm. we purified eip18 by washing the amorphous particles with the mixture of triton x-100, nacl and ethylenediaminetetraacetic acid (edta). we determined several partial am ... | 1997 | 9177316 |
| comparative study of the fermentative characteristics of inulin and different types of fibre in rats inoculated with a human whole faecal flora. | it is known that the physico-chemical characteristics of fibre modify their fermentation characteristics in the colon. previously we showed the varying effects of inulin and different types of fibre on the hepatic and intestinal xenobiotic-metabolizing enzymes (xme) in initially germ-free rats inoculated with a human, methanogenic, whole-faecal flora (roland et al. 1994). the aim of the present work was to assess whether or not these effects could be related to differences in production of ferme ... | 1995 | 7547841 |
| plant 21d7 protein, a nuclear antigen associated with cell division, is a component of the 26s proteasome. | previously, we cloned a carrot (daucus carota l.) cdna encoding a 45-kd protein, 21d7, located in the nuclei of proliferating cells. the 21d7 protein is similar to the partial sequence of a regulatory subunit of the bovine 26s proteasome, p58 (g. demartino, c.r. moomaw, o.p. zagnitko, r.j. proske, m. chu-ping, s.j. afendis, j.c. swaffield, c.a. slaughter [1994] j biol chem 269: 20878-20884) and to the deduced sequence encoded by the saccharomyces cerevisiae gene sun2 (m. kawamura, k. kominami, j ... | 1997 | 9008397 |
| isoforms of chalcone synthase in daucus carota l. and their differential expression in organs from the european wild carrot and in ultraviolet-a-irradiated cell cultures. | two isoforms of chalcone synthase (chs) were isolated from cdna libraries derived from uv-a-irradiated anthocyanin-accumulating (dcb) and non-accumulating (dcs) cell cultures of carrot (daucus carota l.). the clones designated as dcchs1, which were present only in the dcb library, had a deduced primary sequence of 389 amino acids and an expected molecular mass of 42.7 kda, and seem to be alleles of those cloned by ozeki et al. (1993). the second isoform (dcchs2) was present in both libraries. it ... | 2000 | 10872233 |
| isolation and characterization of a diverse set of genes from carrot somatic embryos. | the early events in plant embryogenesis are critical for pattern formation, since it is during this process that the primary apical meristems and the embryo polarity axis are established. however, little is known about the molecular events that are unique to the early stages of embryogenesis. this study of gene expression during plant embryogenesis is focused on identifying molecular markers from carrot (daucus carota) somatic embryos and characterizing the expression and regulation of these gen ... | 1996 | 8938424 |
| complementary immunolocalization patterns of cell wall hydroxyproline-rich glycoproteins studied with the use of antibodies directed against different carbohydrate epitopes. | antisera raised against the major hydroxyproline-rich glycoprotein (hrgp) in carrot (daucus carota l.) taproot, extensin-1, and a minor hrgp, extensin-2, were characterized by western blot analysis, enzyme-linked immunosorbent assay, and periodate oxidation and found to be directed against carbohydrate epitopes shared by both glycoproteins. the anti-extensin-1 antibodies (ge1) target periodate-sensitive epitopes and may recognize the terminal alpha-1,3-arabinoside of extensin-1. the anti-extensi ... | 1993 | 7506427 |
| rolb expression pattern in the early stages of carrot somatic embryogenesis. | the pattern of expression of the rolb gene, derived from the t-dna of the plant pathogen agrobacterium rhizogenes, has been investigated during the early stages of somatic embryo formation in suspension cultures of carrot (daucus carota l.). the reporter gene gus (escherichia coli beta-glucuronidase), under transcriptional control of full-length rolb promoter region, has been utilized in order to evaluate both qualitative and quantitative variations in the expression pattern. fluorimetric measur ... | 1997 | 9570894 |
| carrot cells contain two top1 genes having the coding capacity for two distinct dna topoisomerases i. | five dna topoisomerase i cdna clones were isolated from a carrot (daucus carota) cdna library and two classes of nucleotide sequences were found. one component of the first class, ptop9, perfectly matches the open reading frame of ptop28, a truncated top1 cdna previously described, and extended it by 594 nucleotides (top1alpha). a member of the second class, ptop11, contains an open reading frame 2727 bp long (top1ss) with a coding capacity for a second putative dna topoisomerase i of 101 kda. b ... | 2000 | 11141172 |
| a novel protein elicitor (panie) from pythium aphanidermatum induces multiple defense responses in carrot, arabidopsis, and tobacco. | a novel protein elicitor (panie(234)) from pythium aphanidermatum (edson) fitzp. was purified, microsequenced, and the corresponding cdna was cloned. the deduced amino acid sequence contains a putative eukaryotic secretion signal with a proteinase cleavage site. the heterologously expressed elicitor protein without the secretion signal of 21 amino acids (panie(213)) triggered programmed cell death and de novo formation of 4-hydroxybenzoic acid in cultured cells of carrot (daucus carota). program ... | 2001 | 11706166 |
| isolation of additional bacteriophages with genomes of segmented double-stranded rna. | eight different bacteriophages were isolated from leaves of pisum sativum, phaseolus vulgaris, lycopersicon esculentum, daucus carota sativum, raphanus sativum, and ocimum basilicum. all contain three segments of double-stranded rna and have genomic-segment sizes that are similar but not identical to those of previously described bacteriophage phi6. all appear to have lipid-containing membranes. the base sequences of some of the viruses are very similar but not identical to those of phi6. three ... | 1999 | 10419946 |
| overexpression of mitochondrial citrate synthase in arabidopsis thaliana improved growth on a phosphorus-limited soil. | the gene for mitochondrial citrate synthase (cs) was isolated from daucus carota (dccs) and introduced into arabidopsis thaliana (strain ws) using agrobacterium tumefaciens-mediated transformation. characteristics of citrate excretion were compared between t3 transgenic plants, which were derived from the initial transgenic plants by self-fertilization and homozygous for dccs, and the control plants that had no dccs. the highest cs activity 0.78 micromol protein min(-1) exhibited by the transgen ... | 2000 | 11100775 |
| the roles of rirol and ngrol genes in hairy root induction in nicotiana debneyi. | the function of rirol genes in tl-dna of the ri plasmid of agrobacterium rhizogenes has been previously studied in nicotiana tabacum and daucus carota, but it was reported that these plants have a tl-dna-similar sequence in their genome. we investigated the function of rirol genes in n. debneyi by infection with a. tumefaciens harboring these genes, because the genome of n. debneyi does not contain a tl-dna-similar sequence. the single gene rirolb induced adventitious roots in n. debneyi. introd ... | 2000 | 11074270 |
| molecular cloning and in situ hybridization of alpha-l-arabinofuranosidase from carrot cells. | the cdna of extracellular alpha-l-arabinofuranosidase (alpha-l-afase, ec 3.2.1.55) secreted from suspension-cultured carrot cells (daucus carota l. cv. kintoki) was isolated and characterized. the nucleotide sequence of the cdna (2.4 kb) revealed an open reading frame consisting of 655 amino acid residues. sequence homology research showed 28.4% identity to the alpha-l-afase a protein of aspergillus niger. the genomic dna was cloned by pcr, and the nucleotide ligature sequence showed 18 exons an ... | 2001 | 12060285 |
| characterization of source- and sink-specific sucrose/h+ symporters from carrot. | to understand how sucrose (suc) is transported from source leaves to developing tap roots of carrot (daucus carota l.), we cloned two cdnas (dcsut1 and dcsut2) for proteins with homologies to plant suc/h+ symporters. the deduced polypeptide sequences are 52% identical and have 12 predicted membrane-spanning domains each. transport activities were confirmed by expression of the clones in yeast cells. both transporters had optimal activity below ph 5.0 and michaelis constant values of 0.5 mm. suc ... | 1998 | 9847123 |
| tolerance and metabolism of phenol and chloroderivatives by hairy root cultures of daucus carota l. | hairy root cultures are shown to be suitable experimental systems to screen higher plants for tolerance to various inorganic and organic pollutants, and for determining the role of the root matrix in the uptake and further metabolism of contaminants. a number of clones were obtained by infection of carrot tissues with agrobacterium rhizogenes and two (the fastest and the slowest growing root clones) were chosen for further experimentation. both clones showed a similar degree of tolerance towards ... | 2002 | 11916048 |
| anti-steroidogenic activity of the petroleum ether extract and fraction 5 (fatty acids) of carrot (daucus carota l.) seeds in mouse ovary. | the petroleum ether extract and fraction 5 (fatty acids) of carrot seeds arrested the normal estrus cycle of adult mouse and reduced the weight of ovaries significantly. the cholesterol and ascorbic acid content in ovaries were significantly elevated due to the treatment with extract and fraction 5 (fatty acids) of carrot seeds. the significant inhibition of delta 5,3-beta-hydroxy steroid dehydrogenase and glucose-6-phosphate dehydrogenase, the two key enzymes involved in ovarian steroidogenesis ... | 1997 | 9292415 |
| assembly and disassembly of the peripheral architecture of the plant cell nucleus during mitosis | the architecture of the nuclei of higher plants includes a structure similar to the nuclear lamina of vertebrates. changes in this structure were monitored during mitosis in carrot (daucus carota l.) and celery (apium graveolens l.) cells by immunofluorescence microscopy using an antibody that recognized the nuclear-matrix protein nmcp1. this protein has been shown to be localized exclusively at the periphery of the nucleus (k. masuda et al. 1997, exp cell res 232: 173-187). immunofluorescence w ... | 1999 | 10592045 |
| metabolism of polyamines in transgenic cells of carrot expressing a mouse ornithine decarboxylase cdna. | the metabolisms of arginine (arg), ornithine (orn), and putrescine were compared in a nontransgenic and a transgenic cell line of carrot (daucus carota l.) expressing a mouse orn decarboxylase cdna. [14c]arg, [14c]orn, and [14c]putrescine were fed to cells and their rates of decarboxylation, uptake, metabolism into polyamines, and incorporation into acid-insoluble material were determined. transgenic cells showed higher decarboxylation rates for labeled orn than the nontransgenic cells. this was ... | 1998 | 9449846 |
| increased putrescine biosynthesis through transfer of mouse ornithine decarboxylase cdna in carrot promotes somatic embryogenesis. | carrot (daucus carota l.) cells were transformed with agrobacterium tumefaciens strains containing 3[prime]-truncated mouse ornithine decarboxylase (odc) cdna under the control of a cauliflower mosaic virus 35s promoter. a neomycin phosphotransferase gene linked with a nopaline synthase promoter was used to select transformed cell lines on kanamycin. although the nontransformed cells contained no odc, high amounts of mouse-specific odc activity were observed in the transformed cells. transgenic ... | 1995 | 12228581 |
| identification and characterization of an 18-kilodalton, vamp-like protein in suspension-cultured carrot cells. | polyclonal antibodies raised against rat vesicle associated membrane protein-2 (vamp-2) recognized, in carrot (daucus carota) microsomes, two major polypeptides of 18 and 30 kd, respectively. a biochemical separation of intracellular membranes by a sucrose density gradient co-localized the two polypeptides as resident in light, dense microsomes, corresponding to the endoplasmic reticulum-enriched fractions. purification of coated vesicles allowed us to distinguish the subcellular location of the ... | 2000 | 10631246 |
| purification and characterization of a soluble phosphatidylinositol 4-kinase from carrot suspension culture cells. | previously we reported the presence of a soluble phosphatidylinositol 4-kinase (pi 4-kinase) in carrot (daucus carota l.) suspension culture cells (c.m. okpodu, w. gross, w.f. boss [1990] plant physiol 93: s-63). we have purified the enzyme over 1000-fold using q-sepharose ion exchange, hydroxylapatite, and g-100 gel filtration column chromatography. the mr of the enzyme was estimated to be 83,000 by gel filtration. pi 4-kinase activity was recovered after renaturation of the 80-kd region of pol ... | 1995 | 12228376 |
| susceptibility of two different strains of aedes aegypti (diptera: culicidae) to plant oils. | the toxicity of 11 oils extracted from plants commonly grown in the himalayan region was studied using larvae of two aedes aegypti (l.) strains. a strain from liverpool, england, was highly susceptible to these oils. the lc50 values were much higher in a local laboratory strain. daucus carota l. oil was highly toxic in both strains. differences in the susceptibility of these strains to the action of the test oils and their potential use in integrated pest management are discussed. | 2004 | 15568366 |
| identification of highly conserved hydrophobic amino acid motif in deduced amino acid sequence of elymus sibiricus l. mitochondrial s13 ribosomal protein. | the nucleotide sequence of mitochondrial ribosomal protein rps13 gene from wild perennial grass elymus sibiricus is presented. it was determined by the method of pcr amplification with specific oligonucleotide primers and the direct sequencing of the amplification product. the sequence of e. sibiricus mitochondrial gene for s13 predicts a hydrophobic ribosomal protein of 116 amino acids that shows strong similarity to those of wheat (99.7% identity) and maize (98%). the deduced amino acid sequen ... | 2000 | 10778389 |
| effect of daucus carota var. boissieri extracts on immune response of schistosoma mansoni infected mice. | isolation and structure elucidation was carried out of flavonoid constituents found in fractionated extracts of the seeds and green leaves of daucus carota l. var. boissieri (apiaceae). the flavonoids are mainly apigenin, luteolin, their glycosidic precursors and 2,4,5-trimethoxybenzaldehyde. fatty acids, hydrocarbons and sterols were identified by glc. the effect of various carrot extracts on the immune responses of schistosoma mansoni infected mice was studied. the rate of reduction in worm in ... | 1999 | 10983240 |
| conservation of the basic pattern of cellular amino acid composition during biological evolution in plants. | the cellular amino acid composition of plant cells was analyzed. the callus of carrot (daucus carota), leaves of torenia fournieri and protocomb-like body of cymbidium, s.p. were examined as examples of plant cells. the cellular amino acid compositions differed in the plant cells, but their basic patterns were quite similar. it is concluded that the basic pattern of the cellular amino acid composition is conserved in all terrestrial organisms, including plants. | 2000 | 10817410 |
| hypotensive action of coumarin glycosides from daucus carota. | daucus carota (carrot) has been used in traditional medicine to treat hypertension. activity-directed fractionation of aerial parts of d. carota resulted in the isolation of two cumarin glycosides coded as dc-2 and dc-3. intravenous administration of these compounds caused a dose-dependent (1-10 mg/kg) fall in arterial blood pressure in normotensive anaesthetised rats. in the in vitro studies, both compounds caused a dose-dependent (10-200 microg/ml) inhibitory effect on spontaneously beating gu ... | 2000 | 11081994 |
| the metabolites of the herbicide l-phosphinothricin (glufosinate) (identification, stability, and mobility in transgenic, herbicide-resistant, and untransformed plants). | the metabolism of the herbicide l-phosphinothricin (l-pt) was analyzed in tobacco (nicotiana tabacum), alfalfa (medicago sativa), and carrot (daucus carota). in transgenic, pt-resistant plants expressing the pt-n-acetyltransferase gene (pat), l-pt was acetylated, resulting in two forms of n-acetyl-pt (ac-pt). in transgenic plants expressing only low pat-encoded acetylating activity as well as in genetically unmodified plants, three metabolic compounds 4-methylphosphinico-2-oxo-butanoic acid, 3-m ... | 1994 | 12232195 |
| soil-to-root transfer and translocation of polycyclic aromatic hydrocarbons by vegetables grown on industrial contaminated soils. | polycyclic aromatic hydrocarbons (pahs) are possible contaminants in some former industrial sites, representing a potential risk to human health if these sites are converted to residential areas. this work was conducted to determine whether pahs present in contaminated soils are transferred to edible parts of selected vegetables. soils were sampled from a former gasworks and a private garden, exhibiting a range of pah concentrations (4 to 53 to 172 to 1263 and 2526 mg pahs kg-1 of dry soil), and ... | 2002 | 12371182 |
| attachment to roots and virulence of a chvb mutant of agrobacterium tumefaciens are temperature sensitive. | agrobacterium tumefaciens chvb mutants are unable to produce beta-1,2 glucan. they are nonattaching and avirulent and show reduced motility at room temperature. at lower temperatures (16 degrees c), chvb mutants became virulent on bryophyllum daigremontiana and lycopersicon esculentum and were able to attach to l. esculentum, arabidopsis thaliana, daucus carota, and tagetes erecta roots. the mutant bacteria also recovered wild-type motility at lower temperatures. two other nonattaching mutants o ... | 2002 | 11876426 |
| over expression of mitochondrial citrate synthase gene improves the growth of carrot cells in al-phosphate medium. | a mitochondrial citrate synthase (cs) of arabidopsis thaliana was introduced into carrot (daucus carota l. cv. ms yonsun) cells by agrobacterium tumefaciens-mediated transformation. transgenic cell lines had high cs activity, the highest value observed was 0.24 mumol (mg protein)-1 min-1 which was 1.9-fold of that in wild-type cells. transcript levels of dccs were similar between transgenic lines, but those of atcs were increased as the cs activity of cells was increased. isoelectric focussing r ... | 1999 | 10427772 |
| calcium and phospholipid activation of a recombinant calcium-dependent protein kinase (dccpk1) from carrot (daucus carota l.). | a calmodulin-like domain protein kinase (dccpk1, previously designated cdpk431) cloned from carrot (daucus carota l.) was expressed at high levels in escherichia coli and partially purified. ca(2+)-induced gel mobility shift and (45)ca(2+) ligand binding assays confirmed that recombinant dccpk1 binds ca(2+) through its calmodulin-like domain and undergoes a significant conformational change. ca(2+) activated the kinase activity of recombinant dccpk1 (k(0.5)=1.7 microm) up to 20-fold. ca(2+) comb ... | 1999 | 10556555 |
| monitoring urban air particulate matter (fractions pm 2.5 and pm 10) genotoxicity by plant systems and human cells in vitro: a comparative analysis. | increased incidence of mortality and sickness due to cardiopulmonary complications has been associated with elevated levels of urban air particles (uap), with an aerodynamic diameter of 10 microm (pm 10) and 2.5 microm (pm 2.5). in the present report alternative plant systems and human cells in vitro are associated with human hazard and genotoxic risk assessment of uap. the genotoxic activities associated with the coarse (pm 10) and the fine fraction (pm 2.5) of airborne particulates have been a ... | 2002 | 12111711 |
| quantitative studies and sensory analyses on the influence of cultivar, spatial tissue distribution, and industrial processing on the bitter off-taste of carrots (daucus carota l.) and carrot products. | although various reports pointed to 6-methoxymellein (1) as a key player imparting the bitter taste in carrots, activity-guided fractionation experiments recently gave evidence that not this isocoumarin but bisacetylenic oxylipins contribute mainly to the off-taste. among these, (z)-heptadeca-1,9-dien-4,6-diyn-3-ol (2), (z)-3-acetoxy-heptadeca-1,9-dien-4,6-diyn-8-ol (3), and (z)-heptadeca-1,9-dien-4,6-diyn-3,8-diol (falcarindiol, 4) have been successfully identified. in the present study, an ana ... | 2004 | 15237959 |
| potyviruses, novel and known, in cultivated and wild species of the family apiaceae in australia. | three potyviruses were identified by gene sequencing and found to be widespread in species of apiaceae in australia. only celery mosaic virus was found in celery crops and in one of 180 specimens of feral carrot ( daucus carota). another related but distinct novel potyvirus, carrot virus y, was the only virus found in carrot crops and all except one feral carrot. a more distantly related novel potyvirus, apium virus y, was found in plants of sea celery ( apium prostratum), cultivated parsley ( p ... | 2002 | 12376749 |
| length of time in tissue culture can affect the selected glyphosate resistance mechanism. | usually, stepwise selection of plant suspension cultures with gradually increasing concentrations of the herbicide glyphosate results in the amplification of the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase (epsps; ec 2.5.1.19) gene that leads to resistance by increasing epsps mrna and enzyme activity. we show that glyphosate selection with newly initiated suspension cultures can produce resistant lines with resistance mechanisms other than gene amplification and that usually as the ... | 2004 | 14566562 |
| elicitor-induced changes in ca2+ influx, k+ efflux, and 4-hydroxybenzoic acid synthesis in protoplasts of daucus carota l. | suspension-cultured carrot cells (daucus carota) and their protoplasts respond to a fungal elicitor prepared from the culture medium of pythium aphanidermatum by accumulating 4-hydroxybenzoic acid (4-hba). protoplasts release the compound into the culture medium. using 45cacl2 as a tracer, we were able to demonstrate that the secretion of 4-hba is preceded by a rapid increase in the ca2+ influx and a concomitant k+ efflux. if the increased ca2+ influx was prevented by ethyleneglycol-bis([beta]-a ... | 1993 | 12231948 |
| [effect of blanching and hygroscopic coating on quality of fresh cut carrots (daucus carota var. chantenay) during storage]. | the effect of blanching at low temperatures (solution 1% acid citric, 50 degrees c for 30 seconds) and the application of glycerol as humectant (3% p/p, 20 seconds) to preserve the quality of fresh-cut carrots (daucus carota) were studied as individual or combined treatments. four treatments were evaluated: a control by dipping samples for 30 seconds in distilled water (t1); blanching (t2); glycerol application (t3); and blanching plus glycerol application (t4). total carotenoids content, color, ... | 2002 | 12184154 |
| calcium levels affect the ability to immunolocalize calmodulin to cortical microtubules. | calcium affects the stability of cortical microtubules (mts) in lysed protoplasts. this calmodulin (cam)-mediated interaction may provide a mechanism that serves to integrate cellular behavior with mt function. to test the hypothesis that cam associates with these mts, monoclonal antibodies were produced against cam, and one (designated mab1d10) was selected for its suitability as an immunocytochemical reagent. it is shown that cam associates with the cortical mts of cultured carrot (daucus caro ... | 1993 | 12231960 |
| biotransformation of isoprenoids and shikimic acid derivatives by a vegetable enzymatic system. | in biotransformations carried out under similar conditions enzymatic systems from carrot (daucus carota l.), celeriac (apium graveolens l. var. rapaceum) and horse-radish (armoracia lapathifolia gilib.) hydrolyzed the ester bonds of acetates of phenols or alicyclic alcohols. nevertheless, methyl esters of aromatic acids did not undergo hydrolysis. alcohols were oxidized to ketones in a reversible reaction. | 2004 | 15241926 |
| abscisic acid-inducible nuclear proteins bind to bipartite promoter elements required for aba response and embryo-regulated expression of the carrot dc3 gene. | the carrot (daucus carota l.) lea-class gene dc3 is expressed in developing seeds and in vegetative tissues subject to drought and treatment with exogenous abscisic acid (aba). cis regulatory elements involved in seed-specific expression and in response to aba were identified in transgenic tobacco (nicotiana tabacum l.) using beta-glucuronidase (gus) reporter gene constructs containing a series of deletion and orientation mutants of the dc3 promoter. these experiments demonstrated that the dc3 p ... | 2005 | 15378369 |
| gibberellin is essentially required for carrot (daucus carota l.) somatic embryogenesis: dynamic regulation of gibberellin 3-oxidase gene expressions. | a ga biosynthesis inhibitor, uniconazole, caused many shrunken embryos when it was supplied to cultured carrot (daucus carota l.) cells at the induction of somatic embryos. the abnormality was prevented by exogenous ga(1) or ga(4). to analyze the status of ga biosynthesis during somatic embryogenesis, expression patterns of newly isolated genes encoding ga biosynthetic enzymes, two ga 20-oxidases, three ga 3-oxidases, and two ga 2-oxidases were observed by using a semi-quantitative reverse-trans ... | 2003 | 14646205 |
| occurrence of gluconacetobacter diazotrophicus in tropical and subtropical plants of western ghats, india. | endophytic bacteria were isolated from the tissues of surface sterilized roots, stems, and leaves of fifty different crop plants. phenotypic, biochemical tests and species-specific pcr assay permitted identification of four isolates of gluconacetobacter diazotrophicus from root tissues of carrot (daucus carota l.), raddish (raphanus sativus l.), beetroot (beta vulgaris l.) and coffee (coffea arabica l.). further the plant growth promoting traits such as nitrogenase activity, production of phytoh ... | 2004 | 15462523 |
| evaluation of fungicides for the control of carrot cavity spot. | cavity spot is one of the most common and serious diseases of carrot (daucus carota l). the disease, caused by different species of pythium, including p. violae chesters & hickman, p. sulcatum pratt & mitchell and p. sylvaticum campbell & hendrix, leads to frequent high rejection rates during grading worldwide. in the area of the city of québec, the disease is caused mainly by p. sulcatum, p. sylvaticum and p. macrosporum vaartaja & van der plaats-niterink. cavity spot can be controlled with met ... | 2005 | 15880371 |
| [studies on the bifidus factor. iii. studies on the various properties of the growth-promoting factor for bifidus bacilli contained in the carrot root (daucus carota)]. | | 1964 | 14124663 |
| effectiveness of lemon juice, vinegar and their mixture in the elimination of salmonella typhimurium on carrots (daucus carota l.). | lemon juice, vinegar and the mixture of lemon juice and vinegar (1:1) were tested for their effectiveness in reducing the counts of inoculated salmonella typhimurium (approximately 6 and 3 log cfu/g) on carrots. treatment of carrot samples with lemon juice vinegar alone for different exposure times (0, 15, 30 and 60 min) caused significant reductions ranging between 0.79-3.95 and 1.57-3.58 log cfu/g, respectively, while the number of pathogens was reduced to an undetectable level after 30-min tr ... | 2004 | 15454320 |
| detection and molecular characterization of an aster yellows phytoplasma in poker statice and queen anne's lace in alberta, canada. | queen anne's lace and poker statice plants were found with a yellows-type disease with typical phytoplasma symptoms in an experimental farm near brooks, alberta in 1996. phytoplasma bodies were detected by transmission electron microscopy in phloem cells of symptomatic plants, but not in healthy plants. the presence of a phytoplasma was confirmed by analysis with the polymerase chain reaction. using a pair of universal primer sequences derived from phytoplasma 16s rrna, an amplified product of t ... | 2004 | 15160606 |