establishment of a soybean (glycine max merr. l) transposon-based mutagenesis repository.soybean is a major crop species providing valuable feedstock for food, feed and biofuel. in recent years, considerable progress has been made in developing genomic resources for soybean, including on-going efforts to sequence the genome. these efforts have identified a large number of soybean genes, most with unknown function. therefore, a major research priority is determining the function of these genes, especially those involved in agronomic performance and seed traits. one means to study gen ...200918855007
leveraging model legume information to find candidate genes for soybean sudden death syndrome using the legume information system.comparative genomics is an emerging and powerful approach to achieve crop improvement. using comparative genomics, information from model plant species can accelerate the discovery of genes responsible for disease and pest resistance, tolerance to plant stresses such as drought, and enhanced nutritional value including production of anti-oxidants and anti-cancer compounds. we demonstrate here how to use the legume information system for a comparative genomics study, leveraging genomic informatio ...200718287696
genomic organization and evolutionary insights on grp and ncr genes, two large nodule-specific gene families in medicago truncatula.deciphering the mechanisms leading to symbiotic nitrogen-fixing root nodule organogenesis in legumes resulted in the identification of numerous nodule-specific genes and gene families. among them, ncr and grp genes encode short secreted peptides with potential antimicrobial activity. these genes appear to form large multigenic families in medicago truncatula and other closely related legume species, whereas no similar genes were found in databases of lotus japonicus and glycine max. we analyzed ...200717849716
a database of simple sequence repeats from cereal and legume expressed sequence tags mined in silico: survey and evaluation.simple sequence repeats (ssrs) or microsatellites are an important class of molecular markers for genome analysis and plant breeding applications. in this paper, the ssr distributions within ests from the legumes soybean (glycine max, representing 135.86 mb), medicago (medicago truncatula, 121.1 mb) and lotus (lotus japonicus, 45.4 mb) have been studied relative to the distributions in cereals such as sorghum (sorghum bicolor, 98.9 mb), rice (oryza sativa, 143.9 mb) and maize (zea mays, 183.7 mb ...200617518768
genome mapping of white clover (trifolium repens l.) and comparative analysis within the trifolieae using cross-species ssr markers.allotetraploid white clover (trifolium repens l.), a cool-season perennial legume used extensively as forage for livestock, is an important target for marker-assisted breeding. a genetic linkage map of white clover was constructed using simple sequence repeat (ssr) markers based on sequences from several trifolieae species, including white clover, red clover (t. pratense l.), medicago truncatula (gaertn.) and soybean (glycine max l.). an f(1) population consisting of 179 individuals, from a cros ...200717356868
a novel family of lectins evolutionarily related to class v chitinases: an example of neofunctionalization in legumes.a lectin has been identified in black locust (robinia pseudoacacia) bark that shares approximately 50% sequence identity with plant class v chitinases but is essentially devoid of chitinase activity. specificity studies indicated that the black locust chitinase-related agglutinin (robpscra) preferentially binds to high-mannose n-glycans comprising the proximal pentasaccharide core structure. closely related orthologs of robpscra could be identified in the legumes glycine max, medicago truncatula ...200717098856
the medicago truncatula sunn gene encodes a clv1-like leucine-rich repeat receptor kinase that regulates nodule number and root length.four medicago truncatula sunn mutants displayed shortened roots and hypernodulation under all conditions examined. the mutants, recovered in three independent genetic screens, all contained lesions in a leucine-rich repeat (lrr) receptor kinase. although the molecular defects among alleles varied, root length and the extent of nodulation were not significantly different between the mutants. sunn is expressed in shoots, flowers and roots. although previously reported grafting experiments showed t ...200516240175
mining est databases to resolve evolutionary events in major crop species.using plant est collections, we obtained 1392 potential gene duplicates across 8 plant species: zea mays, oryza sativa, sorghum bicolor, hordeum vulgare, solanum tuberosum, lycopersicon esculentum, medicago truncatula, and glycine max. we estimated the synonymous and nonsynonymous distances between each gene pair and identified two to three mixtures of normal distributions corresponding to one to three rounds of genome duplication in each species. within the poaceae, we found a conserved duplica ...200415499401
estimating genome conservation between crop and model legume species.legumes are simultaneously one of the largest families of crop plants and a cornerstone in the biological nitrogen cycle. we combined molecular and phylogenetic analyses to evaluate genome conservation both within and between the two major clades of crop legumes. genetic mapping of orthologous genes identifies broad conservation of genome macrostructure, especially within the galegoid legumes, while also highlighting inferred chromosomal rearrangements that may underlie the variation in chromoso ...200415489274
the down-regulation of mt4-like genes by phosphate fertilization occurs systemically and involves phosphate translocation to the shoots.mt4 is a cdna representing a phosphate-starvation-inducible gene from medicago truncatula that is down-regulated in roots in response to inorganic phosphate (pi) fertilization and colonization by arbuscular mycorrhizal fungi. split-root experiments revealed that the expression of the mt4 gene in m. truncatula roots is down-regulated systemically by both pi fertilization and colonization by arbuscular mycorrhizal fungi. a comparison of pi levels in these tissues suggested that this systemic down- ...19999880366
[cloning and characterization of 3-hydroxy-3-methylglutary coa reductase cdna of glycyrrhiza uralensis].to clone and analysis the sequence of 3-hydroxy-3-methylglutary coa reductase (hmgr) cdna from glycyrrhiza uralensis.201121837964
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