sequence-tagged microsatellite site markers for chickpea (cicer arietinum l.). | two small-insert genomic libraries of chickpea (cicer arietinum l.) were screened with a set of microsatellite-specific oligonucleotide probes. a total of 121 positive clones were identified among 13,000 plated colonies. thirty-nine clones were recognized by (taa)5, 26 by (ga)8, 18 by (gt)8, 27 by a pool of at-rich trinucleotide repeats [(caa)5, (cat)5, and (gaa)5], and 11 by a pool of gc-rich trinucleotides [(tcc)5, (cac)5, (cag)5, and (cga)5]. of 53 clones selected for sequencing, 43 carried a ... | 1999 | 10231957 |
immobilization and characterization of beta-galactosidase from the plant gram chicken bean (cicer arietinum). evolution of its enzymatic actions in the hydrolysis of lactose. | beta-galactosidase (beta-d-galactosidase galactohydrolase, ec 3.2.1. 23) isolated and purified from gram chicken bean was immobilized on cross-linked polyacrylamide gel. the activity yield was high and attained up to 72%. compared with the free enzyme, the immobilized enzyme had a wider operational ph range and better thermal stability. lyophilized pieces exhibited good stability when stored at room temperature for 60 days and a favorable operational stability when used eight times repeatedly wi ... | 1999 | 10552372 |
effect of processing methods on the calcium, phosphorus, and phytic acid contents and nutritive utilization of chickpea (cicer arietinum l.). | the effect of chickpea (cicer arietinum l.) processing methods on the nutritive utilization of calcium and phosphorus and on phytic acid, a seed component that affects mineral utilization, was studied. chemical and biological methods were used for nutritional determinations in growing rats. the digestive utilization of calcium from raw chickpea was adequate for growing rats; however, processing resulted in a slight decrease. the metabolic utilization of chickpea calcium was low because of the lo ... | 1999 | 10552569 |
enhancement of beta-glucosidase and beta-galactosidase of trigonella foenum-graecum by exposure to the allelochemical mimosine. | glycohydrolases assume significance in the metabolism of biological systems and have important industrial applications in the areas of pharmaceuticals, food, and medicine. glycosidases were screened in germinating seeds, and attempts were made to enhance their levels. screening of glycosidases in the seedlings during a 72 h germination period revealed higher levels of beta-glucosidase and beta-galactosidase in trigonella foenum-graecum compared to cicer arietinum and vigna radiata. activity of b ... | 1999 | 10563917 |
biochemical controls of citrate synthase in chickpea bacteroids. | bacteroids formed by mesorhizobium ciceri cc 1192 in symbiosis with chickpea plants (cicer arietinum l.) contained a single form of citrate synthase [citrate oxaloacetate-lyase (coa-acetylating) enzyme; ec 4.1.3.7], which had the same electrophoretic mobility as the enzyme from the free-living cells. the citrate synthase from cc 1192 bacteroids had a native molecular mass of 228 +/- 32 kda and was activated by kcl, which also enhanced stability. double reciprocal plots of initial velocity agains ... | 2000 | 10648103 |
plant beneficial effect of two strains of proteus vulgaris isolated from tea plantations. | two strains of proteus isolated from tea plantation soil were tested for their ability to colonise the roots of gram (cicer arietinum), bean (phaseolus radiatus) and mung (phaseolus mungo) using a gnotobiotic system. seeds bacterized with the two strains grew faster and showed significant increase in root and shoot enlargement of the plants tested. the bioactive fractions obtained from the culture filtrates and separated through hplc showed that the plant growth promoting fractions were not alwa ... | 1999 | 10687288 |
characterization of cdnas encoding two glycine-rich proteins in chickpea (cicer arietinum l.): accumulation in response to fungal infection and other stress factors. | in chickpea plants infected with the pathogenic fungus ascochyta rabiei [pass.] labr. several mrnas for two glycine-rich proteins (grps) were identified by differential cdna screening. the main part of the deduced amino acid sequences of the 14.6 kd grp1 and the larger grp2 consists of glycine-rich repetitive elements essentially as found for grps in other plants. tyrosine residues in conserved positions inside these repetitive motifs suggest an involvement of the grps in a polymerization proces ... | 2000 | 10725561 |
cloning and characterization of eight cytochrome p450 cdnas from chickpea (cicer arietinum l.) cell suspension cultures. | eight different p450 sequences were isolated from a cdna library derived from cultured chickpea cells (cultivar ilc3279) elicited with a phytophthora sojae (formerly megasperma) elicitor (pmg-elicitor) by screening with heterologous and homologous probes. screening with cyp73a1 from helianthus tuberosus yielded several clones with one identical sequence. a full-length clone could be isolated and this sequence was assigned cyp73a19. heterologous expression in yeast confirmed that cyp73a19 is the ... | 2000 | 10773344 |
assessing phytoestrogen exposure in epidemiologic studies: development of a database (united states). | phytoestrogens (weak estrogens found in plants or derived from plant precursors by human metabolism) have been hypothesized to reduce the risk of a number of cancers. however, epidemiologic studies addressing this issue are hampered by the lack of a comprehensive phytoestrogen database for quantifying exposure. the purpose of this research was to develop such a database for use with food-frequency questionnaires in large epidemiologic studies. | 2000 | 10843440 |
characterization of an atrazine-degrading pseudaminobacter sp. isolated from canadian and french agricultural soils. | atrazine, a herbicide widely used in corn production, is a frequently detected groundwater contaminant. fourteen bacterial strains able to use this herbicide as a sole source of nitrogen were isolated from soils obtained from two farms in canada and two farms in france. these strains were indistinguishable from each other based on repetitive extragenic palindromic pcr genomic fingerprinting performed with primers eric1r, eric2, and boxa1r. based on 16s rrna sequence analysis of one representativ ... | 2000 | 10877767 |
effect of aluminum on the production of siderophore by rhizobium sp. (cicer arietinum). | rhizobium sp. strain bicc 651 in the presence of 100 microm al3+ produced a threefold higher level of siderophore than in the control culture under iron limitation during the stationary phase. al3+ in increasing concentrations resulted in decreased growth, and the effect was alleviated by the addition of iron. siderophore production decreased gradually in al3+-treated culture as well as in the control with the addition of increasing concentrations of fe3+, and at 50 microm fe3+ the level of side ... | 2000 | 10919391 |
optimization of the solid state fermentation process to obtain tempeh from hardened chickpeas (cicer arietinum l.). | solid state fermentation (ssf) represents a technological alternative for a great variety of cereals and legumes, or combination of them, to improve their nutritional quality and to obtain edible products with palatable sensorial characteristics. the objective of this work was to find the best conditions of fermentation temperature and time to obtain tempeh from hardened chickpeas (cicer arietinum l.) applying ssf. response surface methodology (rsm) was applied over three response variables (phy ... | 2000 | 11030476 |
hard-to-cook phenomenon in chickpeas (cicer arietinum l): effect of accelerated storage on quality. | storage, at high temperature (> or = 25 degrees c) and high relative humidity (> or = 65%), causes development of hard to cook (htc) phenomenon in grain legumes. the objective of this work was to study the effect of storage simulating tropical conditions on chickpeas quality. the hardening of the surutato 77, mocorito 88, and blanco sinaloa 92 chickpea varieties was produced using adverse storage (32 +/- 1 degrees c, rh = 75%, 160 days) conditions. for all samples, the hunter 'l' values decrease ... | 2000 | 11030477 |
hemagglutinins in vegetative tissues of bengal gram. | hemagglutinating activity was identified in the roots and vegetative tissues of bengal gram using rabbit erythrocytes. the activity in the roots appears to be similar to that of the seeds in respect to their sugar inhibition property. seed and root extracts were found to have fetuin inhibitory agglutinating activity, whereas cotyledons showed different sugar specificity. however, 7-day cotyledons and roots from 3 and 7-day plants were also found to have galactose and n-acetyl-d-galactosamine inh ... | 2000 | 11030478 |
[obtaining a fermented chickpea extract (cicer arietinum l.) and its use as a milk extensor]. | chickpea (cicer arietinum l) is cultivated in the north part of méxico and it is considered a good source of vegetal protein of low cost (20% average), nevertheless, the 80% used for the exportation and only the 20% less was used for animal feeding. the main objective in this study is to obtain a fermented chickpea extract for using in dairy extensor. chickpea water absorbtion kinetics were carried out in e temperature conditions:while the conditions were established, chickpea was grounded and f ... | 2000 | 11048588 |
influence of legume blends on fried papad quality. | legumes and their blends are widely used for the production of papads. papads with low fat content would be a boon to populations looking for low-calorie foods with retention of organoleptic profile. judicious blending of legumes such as black gram, green gram, bengal gram, red gram and cowpea revealed that low-fat fried papads could be prepared from a blend of 40:36:24 blend of bengal gram:black gram:green gram flours. the blend had 15.6% lower fat content as compared to the control prepared fr ... | 2000 | 11103303 |
gas exchange by pods and subtending leaves and internal recycling of co(2) by pods of chickpea (cicer arietinum l.) subjected to water deficits. | terminal drought markedly reduces leaf photosynthesis of chickpea (cicer arietinum l.) during seed filling. a study was initiated to determine whether photosynthesis and internal recycling of co(2) by the pods can compensate for the low rate of photosynthesis in leaves under water deficits. the influence of water deficits on the rates of photosynthesis and transpiration of pods and subtending leaves in chickpea (cv. sona) was investigated in two naturally-lit, temperature-controlled glasshouses. ... | 2001 | 11181721 |
isolation and characterisation of legumin promoter sequence from chickpea (cicer arietinum l.). | seed specific promoters are useful for expression of foreign genes in the seeds. we have isolated a cicer arietinum legumin promoter from lambda embl genomic library and subcloned in pbluescript ii ks (-) in eco rv and pst i site. the 2.762 kb hind ii pst i fragment was sequenced completely by dideoxy chain termination method by creating a set of unidirectional deletions of the inserts in pakkiii. the insert contains mainly upstream sequence (2240 bps) and only a part of structural gene (522 bps ... | 2000 | 11218814 |
symbiotic effectiveness of spontaneous antibiotic-resistant mutants of rhizobium sp. cicer nodulating chickpea (cicer arietinum). | spontaneous streptomycin-resistant mutants were isolated from two fast growing gum-producing strains ca85 and ca401 and from two moderately growing strains ca181 and ca534 of rhizobium sp. cicer. the nodulation ability and symbiotic effectiveness of the mutants relative to parent strains were evaluated on chickpea (cicer arietinum) grown in sterilized chillum jars. some mutants of strains ca85 and ca401 showed nod phenotype whereas some mutants of strains ca181 and ca534 showed nod(+) fix(-) phe ... | 2001 | 11297364 |
effects of salinity on protein and lipopolysaccharide pattern in a salt-tolerant strain of mesorhizobium ciceri. | to characterize the physiological and metabolic responses of mesorhizobium ciceri strain ch-191 to salt stress, investigating the changes induced by salinity in protein and lipopolysaccharide profiles, as well as determining the accumulation of amino acids, glutamate and proline. | 2001 | 11298245 |
effects of rhizobacteria and root symbionts on the reproduction of meloidogyne javanica and growth of chickpea. | the effects of rhizobacteria, i.e. pseudomonas fluorescens, azotobacter chyroococcum and azospirillum brasilense, alone and in combination with root symbionts, rhizobium sp. and glomus mosseae, on the growth of chickpea, cicer arietinum, and reproduction of meloidogyne jaranica were studied. when added alone g. mosseae was better at improving plant growth and reducing galling and nematode reproduction than any other tested organism. application of p. fluorescens caused an almost similar increase ... | 2001 | 11396906 |
nutritional value of raw and autoclaved kabuli and desi chickpeas (cicer arietinum l.) for growing chickens. | 1. two experiments were carried out to determine the effect of inclusion of raw (kabuli and desi) and autoclaved (desi) chickpea seeds in wheat-based starter diets in chickens grown to 28 d of age on the performance, digestive organ sizes, nitrogen-corrected apparent metabolisable energy (amen), ileal apparent protein and starch digestibilities (apd and asd) and intestinal alpha-amylase and trypsin activities. 2. in the first experiment, diets were formulated to contain 0, 150, 300 and 450 g/kg ... | 2001 | 11421334 |
rhizobial survival and nodulation of chickpea as influenced by fungicide seed treatment. | the survival of rhizobium ciceri on chickpea (cicer arietinum cv. myles) seed, treated separately with 1 of 4 commercial fungicides, i.e., apron, arrest 75w, crown, or captan, was examined under laboratory conditions using standard serial dilution and plate count techniques. the resulting effects of fungicide-rhizobium interactions on nodulation, n2 fixation, and plant growth were assessed in a controlled environment. fungicide treatment decreased the number of viable rhizobia on the seed. in ge ... | 2001 | 11467735 |
effects of isoflavonoids from cicer on larvae of heliocoverpa armigera. | four recently identified isoflavonoids, isolated from wild relatives of chickpea, cicer arietinum, were shown to deter larval feeding by heliocoverpa armigera at 100 ppm, judaicin and maackiain retained their antifeedant activity at 50 ppm and 10 ppm, respectively. the isoflavonoids were tested in combinations and with chlorogenic acid; the combinations containing judaicin and maackiain were most active, and chlorogenic acid enhanced the antifeedant activity of all four isoflavonoids. h. armiger ... | 2001 | 11471948 |
some furfural derivatives as nitrification inhibitors. | three series of furfural derivatives, namely n-o-furfural oxime ethers, furfural schiff bases (furfurylidene anilines), and furfural chalcones, have been synthesized and evaluated for nitrification inhibition activity in laboratory incubation studies in typic ustocrept soil. furfural oxime ethers and furfural schiff bases showed potential activity, but furfural chalcones were only mildly active. n-o-ethyl furfural oxime among the oxime ethers, and furfurylidine-4-chloroaniline among the furfural ... | 2001 | 11600014 |
analysis of the distribution of copper amine oxidase in cell walls of legume seedlings. | copper-containing amine oxidase (cuao) has been proposed to play a role in h2o2 production in plant cell walls during cell development and in response to pathogen attack. we have compared the localisation of cuao in pea (pisum sativum l.), lentil (lens culinaris m.) and chick pea (cicer arietinum l.) grown under different light conditions, using both immuno- and histochemical techniques. the enzyme was detected by indirect immunofluorescence in the cell walls of parenchyma tissues of etiolated p ... | 2001 | 11762169 |
chitinolytic and cellulolytic pseudomonas sp. antagonistic to fungal pathogens enhances nodulation by mesorhizobium sp. cicer in chickpea. | pseudomonas strains isolated from the rhizosphere of chickpea (cicer arietinum l.) and green gram (vigna radiata l.) were screened for the production of chitinases and cellulases. five pseudomonas strains were found to produce appreciable amounts of both enzymes in culture-free supernatants and showed growth inhibition of the two fungi pythium aphanidermatum (oomycete) and rhizoctonia solani (basidiomycete) in plates on potato dextrose agar medium. the fungal growth inhibition was not correlated ... | 2001 | 11770853 |
biochemical and molecular biological studies on infection (ascochyta rabiei)-induced thaumatin-like proteins from chickpea plants (cicer arietinum l.). | a pathogenesis-related protein induced by infection with ascochyta rabiei was purified from intercellular washing fluid of chickpea (cicer arietinum l.) leaves. amino-terminal sequencing identified the protein, named pr-5a, as a thaumatin-like protein. the isoelectric point was determined with 6.5 and the molecular mass is 16 kda. therefore, chickpea pr-5a is the first dicot member of a tlp subgroup containing small tlps with a molecular weight between 15 and 18 kda. pr-5a shows no antifungal ac ... | 2001 | 11837663 |
isolation of a new cyclophilin-like protein from chickpeas with mitogenic, antifungal and anti-hiv-1 reverse transcriptase activities. | a protein designated chickpea cyclophilin-like antifungal protein (clap) was isolated from seeds of the chickpea (cicer arietinum). chickpea clap was characterized by a molecular weight of 18 kda and an n-terminal sequence homologous to cyclophilins. the protein was isolated with a procedure involving affinity chromatography on affi-gel blue gel and ion exchange chromatography on cm-sepharose. in addition to an inhibitory effect on the growth of fungi including rhizoctonia solani, mycosphaerella ... | 2002 | 11848297 |
copper amine oxidase expression in defense responses to wounding and ascochyta rabiei invasion. | wounding chickpea (cicer arietinum) internodes or cotyledons resulted in an increase in the steady-state level of copper amine oxidase (cuao) expression both locally and systemically. dissection of the molecular mechanisms controlling cuao expression indicated that jasmonic acid worked as a potent inducer of the basal and wound-inducible cuao expression, whereas salicylic acid and abscisic acid caused a strong reduction of the wound-induced cuao expression, without having any effect on the basal ... | 2002 | 11891243 |
enzymes of malate metabolism in mesorhizobium ciceri cc 1192. | electrophoretic studies were performed on enzymes concerned with the oxidation of malate in free-living and bacteroid cells of mesorhizobium ciceri cc 1192, which forms nitrogen-fixing symbioses with chickpea (cicer arietinum l.) plants. two malate dehydrogenases were detected in extracts from both types of cells in native polyacrylamide electrophoresis gels that were stained for enzyme activity. one band of malate dehydrogenase activity was stained only in the presence of nadp+, whereas the oth ... | 2002 | 12030699 |
cicerin and arietin, novel chickpea peptides with different antifungal potencies. | two antifungal peptides with novel n-terminal sequences, designated cicerin and arietin were isolated from seeds of the chickpea (cicer arietinum), respectively. both peptides were adsorbed on affi-gel blue gel and cm-sepharose and exhibited a molecular weight of approximately 8.2 and 5.6 kda, respectively. arietin was more strongly adsorbed on cm-sepharose than cicerin and manifested a higher translation-inhibiting activity in a rabbit reticulocyte lysate system and a higher antifungal potency ... | 2002 | 12084511 |
studies on exudate-depleted sclerotial development in sclerotium rolfsii and the effect of oxalic acid, sclerotial exudate, and culture filtrate on phenolic acid induction in chickpea (cicer arietinum). | exudate depletion from developing sclerotia of sclerotium rolfsii sacc. in culture caused reduced size and weight of sclerotia. germination of exudate-depleted sclerotia was delayed on cyperus rotundus rhizome meal agar medium when compared with that of control sclerotia. the exudate-depleted sclerotia caused infection in chickpea (cicer arietinum) plants in a glasshouse. different temperatures and incubation periods had no effect on the germination ability of the exudate-depleted sclerotia. oxa ... | 2002 | 12109884 |
survival of the rhizosphere-competent biocontrol strain pseudomonas fluorescens nbri2650 in the soil and phytosphere. | pseudomonas fluorescens nbri2650 was isolated after screening 360 bacterial strains from the rhizosphere of chickpea (cicer arietinum l.) grown in fungal-disease-suppressive field soil. the strain was selected because of its high rhizosphere competence and ability to inhibit the growth of fusarium oxysporum f.sp. ciceri, rhizoctonia bataticola, and pythium sp. under in vitro conditions. survival and colonization of nbri2650 in the phytosphere of chickpea, cotton (gossypium hirsutum l.), cucumber ... | 2002 | 12224558 |
phenotypic and molecular characterization of chickpea rhizobia isolated from different areas of morocco. | to determine the biodiversity of rhizobial strains nodulating cicer arietinum l. in representative soils from various areas of morocco. | 2002 | 12234335 |
preincubation of mesorhizobium ciceri with flavonoids improves its nodule occupancy. | strains of m. ciceri, symbionts of chickpea (cicer arietinum) were incubated with the flavonoids naringenin, daidzein and quercetin which have earlier been reported as inducers and inhibitors of nodabc-lacz fusion of m. ciceri. preincubation of m. ciceri with naringenin and daidzein (100 nmol/l) for 1 d improved the competitive ability of the inoculated strain while preincubation with quercetin decreased the nodule occupancy of inoculated strain under sterile conditions. under non-sterile condit ... | 2002 | 12503401 |
effect of plant growth-promoting rhizobacteria and culture filtrate of sclerotium rolfsii on phenolic and salicylic acid contents in chickpea (cicer arietinum). | two plant growth-promoting rhizobacteria (pgpr), viz., pseudomonas fluorescens strain pf4 and p. aeruginosa strain pag, protected chickpea ( cicer arietinum) plants from sclerotium rolfsii infection when applied singly or in combination as seed treatment. pag gave the best protection to the seedlings, applied either singly (mortality 16%) or in combination with pf4 (mortality 17%) compared with 44% and 24% mortality in control and pf4 treatment, respectively. the two pgpr strains induced the syn ... | 2003 | 12520369 |
analysis of genetic relationships among perennial and annual cicer species growing in turkey using rapd markers. | random amplified polymorphic dna (rapd) fragments were used to assess genetic relationships among cicer spp. growing in turkey. seven 10-mer primers selected from a 50 random oligonucleotide primer set, depending on their ability to amplify genomic dna in all species, were used to detect rapd variation in 43 wild and cultivated accessions representing ten species. these primers yielded 95 reproducible amplification products, 92 of which were polymorphic. pairwise genetic distances of accessions ... | 2002 | 12582902 |
integration of sequence tagged microsatellite sites to the chickpea genetic map. | fifty sequence-tagged microsatellite site (stms) markers and a resistant gene-analog (rga) locus were integrated into a chickpea ( cicer arietinum l., 2n = 2 x = 16 chromosomes) genetic map that was previously constructed using 142 f(6)-derived recombinant inbred lines (rils) from a cross of c. arietinum x cicer reticulatum lad. the map covers 1,174.5 cm with an average distance of 7.0 cm between markers in nine linkage groups (lgs). nine markers including the rga showed distorted segregation ( ... | 2002 | 12582909 |
genetic variability of fusarium wilt pathogen isolates of chickpea (cicer arietinum l.) assessed by molecular markers. | genetic variability among 43 isolates of fusarium oxysporum f.sp. ciceri, the chickpea wilt pathogen, collected from nine states of india including the four well-characterized races of the pathogen were assessed using the molecular markers, rapds and aflp. principal coordinate analysis of the similarity index data generated from the molecular marker studies mostly gave three different clusters: of these two clusters represented race-1 and race-2, and the third cluster consisted of race-3 and rac ... | 2002 | 12617504 |
molecular markers closely linked to fusarium resistance genes in chickpea show significant alignments to pathogenesis-related genes located on arabidopsis chromosomes 1 and 5. | a population of 131 recombinant inbred lines from a wide cross between chickpea ( cicer arietinum l., resistant parent) and cicer reticulatum (susceptible parent) segregating for the closely linked resistances against fusarium oxysporum f.sp. ciceri races 4 and 5 was used to develop dna amplification fingerprinting markers linked to both resistance loci. bulked segregant analysis revealed 19 new markers on linkage group 2 of the genetic map on which the resistance genes are located. closest link ... | 2003 | 12709786 |
genetic dissection of pathotype-specific resistance to ascochyta blight disease in chickpea (cicer arietinum l.) using microsatellite markers. | ascochyta blight is an economically important disease of chickpea caused by the fungus ascochyta rabiei. the fungus shows considerable variation for pathogenicity in nature. however, studies on the genetics of pathotype-specific resistance are not available for this plant-pathosystem. the chickpea landrace ilc 3279 has resistance to pathotype i and ii of the pathogen. in order to understand the inheritance of pathotype-specific resistance in this crop, both mendelian and quantitative trait loci ... | 2003 | 12748770 |
an intraspecific linkage map of the chickpea ( cicer arietinum l.) genome based on sequence tagged microsatellite site and resistance gene analog markers. | an intraspecific linkage map of the chickpea genome based on stms as anchor markers, was established using an f(2) population of chickpea cultivars with contrasting disease reactions to ascochyta rabiei (pass.) lab. at a lod-score of 2.0 and a maximum recombination distance of 20 cm, 51 out of 54 chickpea-stms markers (94.4%), three issr markers (100%) and 12 rga markers (57.1%) were mapped into eight linkage groups. the chickpea-derived stms markers were distributed throughout the genome, while ... | 2003 | 12750788 |
[legume cross-reactivity]. | legumes are dicotyledonous plants belonging to the fabales order. the main distinctive characteristic of which is their fruit (legumen, seeds contained in pods). this botanical order is formed by three families: mimosaceae, caesalpiniaceae and papilionaceae or fabacea. the papilionaceae family includes the most important allergenic species: lens culinaris (lentil), cicer arietinum (chick-pea), pisum sátivum (pea), arachis hipogea (peanut), phaseolus vulgaris (bean) y glycine max (soy). legumes a ... | 2003 | 12783766 |
economy of fertilizer nitrogen through organic sources in rain-fed rice-legume cropping systems in west bengal, india. | field experiments were conducted at a farmers" plot adjacent to the regional research station, red and laterite zone, sub-center sekhampur (birbhum district) of west bengal, india, situated 23 degrees 24' n latitude, 87 degrees 24' e longitude, to study the effect of different bio- and organic sources of nutrients instead of total fertilizer n in terms of crop productivity in the sequence and building up of soil fertility. during the wet seasons of 1997 and 1998, 12 combinations of bio- and orga ... | 2001 | 12805756 |
upregulation of isoflavonoids and soluble proteins in edible legumes by light and fungal elicitor treatments. | in this study, our working hypothesis was that continuous light and fungal elicitation treatment of legume seedlings would lead to enhanced levels of isoflavonoids and soluble proteins. | 2003 | 12816625 |
cicerarin, a novel antifungal peptide from the green chickpea. | a peptide designated cicerarin, with an n-terminal amino acid sequence vkstgradddlavktkylpp dissimilar from known proteins and peptides and a molecular mass of 8kda, was isolated from seeds of the green chickpea cicer arietinum cv green chickpea. cicerarin was isolated with a procedure that involved ion exchange chromatography on deae-cellulose, affinity chromatography on affi-gel blue gel, and gel filtration by fast protein liquid chromatography on superdex 75. cicerarin was unadsorbed on deae- ... | 2003 | 12895650 |
carbohydrates digestibility and faecal nitrogen excretion in rats fed raw or germinated faba bean (vicia faba)- and chickpea (cicer arietinum)-based diets. | raw or germinated faba bean (vicia faba minor var. alameda) and chickpea (cicer arietinum kabuli var. athenas) seed meals were incorporated in essential amino acid-supplemented and energy-equalized diets for growing (65 (sd 1) g) male wistar rats as the only sources of dietary protein. a lactalbumin-based diet was used as the control. faecal dry weight and n excretion of animals fed legume-containing diets were greater (p<0.01) and dm digestibility lower (p<0.01) than controls. apparent faecal d ... | 2003 | 12908890 |
influence of organic wastes and seasonal environmental factors on growth and reproduction of eisenia fetida. | epigeic earthworms (e. fetida) were cultured on variety of organic wastes amended with cattle manure to determine the influence of diets and the seasonal environmental factors on growth and reproduction. the results showed that growth and reproductive strategies of e. fetida varied with different diets and seasons. growth and reproduction of worms in all wastes were significantly more in winter and monsoon than in summer season. hence winter and monsoon seasons could be considered congenial for ... | 2003 | 12974416 |
effect of bengal gram on experimentally induced high levels of cholesterol in tissues and serum in albino rats. | | 1964 | 14227496 |
novel fermentation media for production of bacillus thuringiensis subsp. israelensis. | the production of bacillus thuringiensis subsp. israelensis (debarjac) (bti) as a biopesticide is not cost-effective using existing fermentation technology. in this study, we explored the use of several less expensive alternative culture media (potato, common sugar, and bengal gram) for the growth and production of bti. growth was obtained in all tested media and was comparable to that obtained in conventional medium (luria-bertani). toxicity assays showed that the toxin produced from the novel ... | 2003 | 14503573 |
[elaboration of "cotija" type cheese made of whole milk and chickpea (cicer arietinum l.) mixture]. | the objective [corrected] of this work was to elaborate "cotija" type cheese prepared with whole milk and chickpea (cicer arietinum l.), inoculated with s. thermophilus and to compare its physicochemical, microbiological and sensorial characteristics with a commercial cheese. two mixture were selected 70:30 and 80:20 (whole milk:chickpea paste). both mixture were ground, pasteurized and inoculated with. s. thermophilus, to develop taste and aroma. two rennet concentrations (1:10,000 and 2:10,000 ... | 2003 | 14528612 |
construction of a hindiii bacterial artificial chromosome library and its use in identification of clones associated with disease resistance in chickpea. | a chickpea ( cicer arietinum l.) bacterial artificial chromosome (bac) library from germplasm line, flip 84-92c, was constructed to facilitate positional cloning of disease resistance genes and physical mapping of the genome. the bac library has 23,780 colonies and was calculated to comprise approximately 3.8 haploid-genome equivalents. studies on 120 randomly chosen clones revealed an average insert size of 100 kb and no empty clones. colony hybridization using the rubp carboxylase large subuni ... | 2004 | 14564396 |
genetic relationships among perennial and annual cicer species growing in turkey assessed by aflp fingerprinting. | aflp markers were used to assess genetic relationships among cicer species with distribution in turkey. genetic distances were computed among 47 cicer accessions representing four perennial and six annual species including chickpea, using 306 positions on aflp gels. aflp-based grouping of species revealed two clusters, one of which includes three perennial species, cicer montbretii, cicer isauricum and cicer anatolicum, while the other cluster consists of two subclusters, one including one peren ... | 2004 | 14605811 |
the chickpea, summer cropping, and a new model for pulse domestication in the ancient near east. | the widely accepted models describing the emergence of domesticated grain crops from their wild type ancestors are mostly based upon selection (conscious or unconscious) of major features related either to seed dispersal (nonbrittle ear, indehiscent pod) or free germination (nondormant seeds, soft seed coat). based on the breeding systems (self-pollination) and dominance relations between the allelomorphs of seed dispersal mode and seed dormancy, it was postulated that establishment of the domes ... | 2003 | 14737827 |
use of a herbicide or lysine plus threonine for non-antibiotic selection of transgenic chickpea. | a desensitized aspartate kinase (ak) gene has been developed as a non-antibiotic selection marker for use in the production of transgenic chickpea ( cicer arietinum l.). transgenic shoots regenerated from embryo explants bombarded with the desensitized ak gene were selected on media containing two amino acids, lysine and threonine (lt). approximately 15% of the putative transgenic shoots of vars. p-362 and p-1042 survived after 4 weeks of growth on msb5 medium (ms mineral salts and b5 vitamins) ... | 2004 | 14749891 |
sensitivity of three leguminous crops to o3 as influenced by different stages of growth and development. | the sensitivity of cicer arietinum, vigna mungo and trigonella foenum-graecum to o(3) has been assessed at different stages of growth and development. plants of different ages (0, 1, 2, 4 and 6 weeks old) were fumigated with 0 and 120 nl litre(-1) o(3), from 09.30 h to 16.30 h each day for four weeks, in hemispherical chambers located out-of-doors. seed germination was not affected by o(3) in any of the species, but there were responses (differing between species) on the cotyledons. true leaves ... | 1991 | 15092157 |
pathotype-specific genetic factors in chickpea (cicer arietinum l.) for quantitative resistance to ascochyta blight. | ascochyta blight in chickpea ( cicer arietinum l.) is a devastating fungal disease caused by the necrotrophic pathogen, ascochyta rabiei (pass.) lab. to elucidate the genetic mechanism of pathotype-dependent blight resistance in chickpea, f(7)-derived recombinant inbred lines (rils) from the intraspecific cross of pi 359075(1) (blight susceptible) x flip84-92c(2) (blight resistant) were inoculated with pathotypes i and ii of a. rabiei. the pattern of blight resistance in the ril population varie ... | 2004 | 15146319 |
the use of phenotypic correlations and factor analysis in determining characters for grain yield selection in chickpea (cicer arietinum l.). | to our knowledge, this is the first report on the use of factor analysis in determining characters for yield selection in chickpea (cicer arietinum l.). the present investigation was undertaken to evaluate yield criteria in chickpea using phenotypic correlations and factor analysis. factor 1 composed of biological yield, reaction to ascochyta blight (ascochyta rabiei (pass.) labr.), plant height, grain yield and harvest index. factor 2 consisted of branches and pods per plant. factor 3 encompass ... | 2004 | 15198713 |
the fusarium oxysporum f. sp. ciceris/cicer arietinum pathosystem: a case study of the evolution of plant-pathogenic fungi into races and pathotypes. | the use of resistant cultivars is one of the most practical and cost-efficient strategies for managing plant diseases. however, the efficiency of resistant cultivars in disease management is limited by pathogenic variability in pathogen populations. knowledge of the evolutionary history and potential of the pathogen population may help to optimize the management of disease-resistance genes, irrespective of the breeding strategy used for their development. in this review, we examine the diversity ... | 2004 | 15248157 |
role of rhizobium (ca-1) inoculation in increasing growth and metal accumulation in cicer arietinum l. growing under fly-ash stress condition. | | 2004 | 15386062 |
an efficient transformation system for chickpea (cicer arietinum l.). | a reproducible and efficient transformation method was developed for desi and kabuli chickpeas (cicer arietinum l.) using germinated seedlings as sources of explants. slices derived from plumules were the most efficient at generating transformed shoots. the agl1 agrobacterium-treated explants were first incubated on thidiazuron-containing media, then selected using phosphinothricin. resistant shoots were successfully transferred to soil either by grafting or in vitro rooting. in experiments each ... | 2004 | 15455257 |
rhizobial strain involvement in plant growth, nodule protein composition and antioxidant enzyme activities of chickpea-rhizobia symbioses: modulation by salt stress. | mesorhizobium ciceri, mesorhizobium mediterraneum and sinorhizobium medicae strains showed different symbiotic performances when inoculated to chickpea (cicer arietinum l., cv. chetoui) at unstressed conditions and under salt stress. the analysis of nodular proteic composition and antioxidant enzyme activities revealed a polymorphism of patterns on sds and native page suggesting a potential dependence on the bacterial partner. salt effect was analysed on plant growth, nitrogen fixation and antio ... | 2004 | 15474377 |
agrobacterium tumefaciens-mediated transformation of chickpea (cicer arietinum l.): gene integration, expression and inheritance. | a reproducible method of agrobacterium-mediated transformation was developed for cicer arietinum (chickpea). initial explants consisted of longitudinal slices from embryonic axes of imbibed, mature seed. the plasmid contained a bi-functional fusion gene conferring both beta-glucuronidase and neomycin phosphotransferase activities, under the control of a 35s35samv promoter. using a series of tissue culture media for co-cultivation, shoot initiation and rooting, we recovered transgenic plants from ... | 2004 | 15503035 |
evaluating legume species as alternative trap crops to chickpea for management of helicoverpa spp. (lepidoptera: noctuidae) in central queensland cotton cropping systems. | mounting levels of insecticide resistance within australian helicoverpa spp. populations have resulted in the adoption of non-chemical ipm control practices such as trap cropping with chickpea, cicer arietinum (l.). however, a new leaf blight disease affecting chickpea in australia has the potential to limit its use as a trap crop. therefore this paper evaluates the potential of a variety of winter-active legume crops for use as an alternative spring trap crop to chickpea as part of an effort to ... | 2004 | 15541187 |
resistant starch derived from processed legumes: in vitro and in vivo fermentation characteristics. | the effect of processing of legumes on resistant starch formation, its rate of fermentation and the production of short chain fatty acids under in vitro and in vivo systems was assessed. the content of resistant starch in pressure-cooked bengal gram, black gram and red gram was 3.59%, 1.58% and 3.34%, respectively. fermentation in vitro of resistant starch derived from processed red gram showed higher amount of short chain fatty acids (2.38 mmol), especially butyric acid (2.22 mmol). under in vi ... | 2004 | 15545048 |
toxicity to cotton boll weevil anthonomus grandis of a trypsin inhibitor from chickpea seeds. | cotton (gossypium hirsutum l.) is an important agricultural commodity, which is attacked by several pests such as the cotton boll weevil anthonomus grandis. adult a. grandis feed on fruits and leaf petioles, reducing drastically the crop production. the predominance of boll weevil digestive serine proteinases has motivated inhibitor screenings in order to discover new ones with the capability to reduce the digestion process. the present study describes a novel proteinase inhibitor from chickpea ... | 2005 | 15649779 |
production of gliotoxin on natural substrates by trichoderma virens. | gliotoxin, an epithiodiketopiperazine toxin produced by the "q" strain of trichoderma virens, is essential for curtailing growth and multiplication of phytopathogens (howell et al. 1993, fravel 1988). three isolates (gv, gv-a and gv-v) of trichoderma virens were grown on natural substrates such as bengal gram hull, gingelly cake, green gram hull, rice bran, soya meal, sugarcane bagasse, soyameal + tapioca, tapioca powder, tapioca peel and wheat bran). it was evident from this study that maximum ... | 2005 | 15678558 |
nutritional quality of microwave-cooked and pressure-cooked legumes. | eight whole legumes, namely bengal gram (cicer arietinum), broad beans (vicia faba), cowpea (vigna catjang), field beans (dolichos lablab), green gram (phaseolus aureus roxb), horse gram (dolichos biflorus), lentils (lens esculenta) and french beans (phaseolus vulgaris), were cooked under pressure or in a microwave oven and were analysed for nutrient composition. raw legumes served as control. the range of nutrients analysed in 100 g cooked samples were as follows: moisture, 62.8-69.7 g; protein ... | 2004 | 15762308 |
a kunitz trypsin inhibitor from chickpea (cicer arietinum l.) that exerts anti-metabolic effect on podborer (helicoverpa armigera) larvae. | chickpea (cicer arietinum l.) seeds contain bowman-birk proteinase inhibitors, which are ineffective against the digestive proteinases of larvae of the insect pest helicoverpa armigera. we have identified and purified a low expressing proteinase inhibitor (pi), distinct from the bowman-birk inhibitors and active against h. armigera gut proteinases (hgp), from chickpea seeds. n-terminal sequencing of this hgp inhibitor revealed a sequence similar to reported pea (pisum sativum) and chickpea alpha ... | 2005 | 15830127 |
bifidogenic effect of dietary fiber and resistant starch from leguminous on the intestinal microbiota of rats. | the bifidogenic effect of leguminous containing diets on the intestinal microbiota of male wistar rats was studied. | 2005 | 15850967 |
molecular cloning and characterisation of a rab-binding gdp-dissociation inhibitor from medicago truncatula. | we have isolated and sequenced the full-length cdna of a gdp-dissociation inhibitor (gdi) from the model legume medicago truncatula l. the cdna (mtgdi) contains an open reading frame of 1335 bp, coding for a protein of 444 amino acids with a calculated molecular mass of 49,785 kda. the deduced amino acid sequence shows significant homology to other plant gdis, the highest homology being found to gdi from the legume cicer arietinum (96% identity). the mtgdi was expressed as a n-terminal flag-fusi ... | 2005 | 15854828 |
paenibacillus rhizosphaerae sp. nov., isolated from the rhizosphere of cicer arietinum. | two sporulating bacterial strains designated cecap06(t) and cecap16 were isolated from the rhizosphere of the legume cicer arietinum in argentina. almost-complete 16s rrna gene sequences identified the isolates as a paenibacillus species. it was most closely related to paenibacillus cineris lmg 18439(t) (99.6 % sequence similarity), paenibacillus favisporus lmg 20987(t) (99.4 % sequence similarity) and paenibacillus azoreducens dsm 13822(t) (97.7 % sequence similarity). the cells of this novel s ... | 2005 | 15879272 |
lead induced changes in antioxidant metabolism of horsegram (macrotyloma uniflorum (lam.) verdc.) and bengalgram (cicer arietinum l.). | one-month old horsegram (macrotyloma uniflorum (lam.) verdc. cv vzm1) and bengalgram (cicer arietinum l. cv annogiri) were exposed to different regimes of lead stress as pb(no3)2 at 0, 200, 500 and 800 ppm concentrations. the extent of oxidative damage as the rate of lipid peroxidation, antioxidative response and the accumulation of lead in roots and shoots of both plants were evaluated after 12 days of lead stress. lead (pb) treated plants showed increased levels of lipid peroxidation as eviden ... | 2005 | 15910908 |
tricalcium phosphate solubilizing abilities of trichoderma spp. in relation to p uptake and growth and yield parameters of chickpea (cicer arietinum l.). | nine isolates of trichoderma spp. were investigated for their ability to solubilize insoluble phosphate in pikovskaya's broth and were compared with an efficient phosphate-solubilizing bacterium bacillus megaterium subsp. phospaticum pb that was used as the reference strain. all 9 trichoderma isolates were found to solubilize insoluble tricalcium phosphate to various extents. trichoderma viride (tv 97) (9.03 microg x ml(-1)), trichoderma virens (pdbctvs 12) (9.0 microg x ml(-1)), and trichoderma ... | 2005 | 15920619 |
in vitro carbohydrate digestibility of whole-chickpea and chickpea bread products. | pulses such as the chickpea are generally considered to be valuable dietary sources of slowly digestible starch, a form of starch that is considered beneficial to health since it results in relatively low post-meal blood glucose levels compared with more rapidly digested starch. the development of novel chickpea-based foods is necessary to help expand the worldwide consumption of the chickpea. however, the effect of different processing methods on the starch digestibility of chickpea-based foods ... | 2005 | 16009629 |
detection of two quantitative trait loci for resistance to ascochyta blight in an intra-specific cross of chickpea (cicer arietinum l.): development of scar markers associated with resistance. | two quantitative trait loci (qtls), (qtl(ar1) and qtl(ar2)) associated with resistance to ascochyta blight, caused by ascochyta rabiei, have been identified in a recombinant inbred line population derived from a cross of kabulixdesi chickpea. the population was evaluated in two cropping seasons under field conditions and the qtls were found to be located in two different linkage groups (lg4a and lg4b). lg4b was saturated with rapd markers and four of them associated with resistance were sequence ... | 2006 | 16328235 |
fly-ash induced synthesis of phytochelatins in chickpea (cicer arietinum l.) plants. | phytochelatins and related metabolites (cysteine and gsh) were found to be induced in the shoots of two varieties of cicer arietinum viz., csg-8962 and c-235 grown under different amendments of fly-ash with garden soil and press mud. cysteine, gsh, pcs and its speciation were found in higher concentrations in amended fly-ash than in the control 100% soil. two species of metal binding peptides i.e., pc2 and pc4 were found in both varieties and in amendments, however, their concentration varied de ... | 2005 | 16334294 |
release of rhizobium spp. from tropical soils and recovery for immunofluorescence enumeration. | limitations associated with immunofluorescence enumeration of bacteria in soil derive largely from the efficiency with which cells can be separated from soil particles and collected on membrane filters for staining. many tropical soils fix added bacteria tightly, resulting in low recoveries. eight soils, representative of three of the major soil orders found in the tropics (oxisols, vertisols, and inceptisols), were tested for recovery of added rhizobium strains. all except one hawaiian andept ( ... | 1981 | 16345824 |
degradation of the isoflavone biochanin a and its glucoside conjugates by ascochyta rabiei. | strains of ascochyta rabiei which are pathogenic to chickpea (cicer arietinum l.) readily catabolized the main chickpea isoflavone biochanin a (5,7-dihydroxy-4'-methoxyisoflavone). 3'-hydroxylation and o-demethylation reactions led to the isoflavones pratensein, genistein, and orobol, which were rapidly further degraded. dihydrogenistein and p-hydroxyphenylacetic acid were also identified as catabolites. biochanin a-7-o-glucoside was degraded, leading to aglycone and pratensein. biochanin a-7-o- ... | 1985 | 16346840 |
crystallization and preliminary x-ray characterization of a lectin from cicer arietinum (chickpea). | the lectin isolated from mature seeds of cicer arietinum (cal) agglutinates pronase-treated rabbit and human erythrocytes and its haemagglutination activity is inhibited by fetuin and desialated fetuin but not by simple monosaccharides or oligosaccharides. the purified lectin is a dimer of molecular weight 43,000 da composed of two identical subunits (mw 21,500), as confirmed by sds-page. the lectin has been crystallized using the hanging-drop vapour-diffusion method at 295 k over a well solutio ... | 2005 | 16508116 |
enzymes of poly-(beta)-hydroxybutyrate metabolism in soybean and chickpea bacteroids. | the enzymatic capacity for metabolism of poly-(beta)-hydroxybutyrate (phb) has been examined in nitrogen-fixing symbioses of soybean (glycine max l.) plants, which may accumulate substantial amounts of phb, and chickpea (cicer arietinum l.) plants, which contain little or no phb. in the free-living state, both bradyrhizobium japonicum cb 1809 and rhizobium sp. (cicer) cc 1192, which form nodules on soybean and chickpea plants, respectively, produced substantial amounts of phb. to obtain informat ... | 1996 | 16535445 |
acetyl coenzyme a acetyltransferase of rhizobium sp. (cicer) strain cc 1192. | to investigate why rhizobium sp. (cicer) strain cc 1192 cells accumulate poly-r-3-hydroxybutyrate in the free-living state but not as bacteroids in nodules on chickpea (cicer arietinum l.) plants, we have examined the kinetic properties of acetyl coenzyme a (acetyl-coa) acetyltransferase (also known as acetoacetyl-coa thiolase and 3-ketothiolase [ec 2.3.1.9]) from both types of cells. the enzyme had a native molecular mass of 180 (plusmn) 4 kda, and the subunit molecular mass was 44 (plusmn) 1 k ... | 1997 | 16535684 |
changes in amino acid profile and metal content in seeds of cicer arietinum l. (chickpea) grown under various fly-ash amendments. | seeds of cicer arietinum l. plants are edible and a valuable source of protein. accumulation of toxic metals in the edible part of the plant, grown in fields close to fly-ash (fa) landfills, may pose a threat to human health. in the present study, the effects of fa and its amendments with different ameliorants viz., garden soil (gs), press mud (pm) and saw dust (sd), on total soluble protein contents, amino acid composition and metal accumulation in seeds were investigated in var. csg-8962 and v ... | 2006 | 16682068 |
expression and localization of calcium-dependent protein kinase isoforms in chickpea. | calcium-dependent protein kinases (cpks) play important roles in multiple signal transduction pathways but the precise role of individual cpk is largely unknown. we isolated two cdnas encoding two cpk isoforms (cicer arietinum cpks-cacpk1 and cacpk2) of chickpea. their expression in various organs and in response to various phytohormones, and dehydration, high salt stress and fungal spore in excised leaves as well as localization in leaf and stem tissues were analyzed in this study. cacpk1 prote ... | 2006 | 16716453 |
expression of cap2, an apetala2-family transcription factor from chickpea, enhances growth and tolerance to dehydration and salt stress in transgenic tobacco. | the apetala2 (ap2) domain defines a large family of dna-binding proteins that play important roles in plant morphology, development, and stress response. we describe isolation and characterization of a gene (cap2) from chickpea (cicer arietinum) encoding a novel ap2-family transcription factor. recombinant cap2 protein bound specifically to c-repeat/dehydration-responsive element in gel-shift assay and transactivated reporter genes in yeast (saccharomyces cerevisiae) one-hybrid assay. cap2 appea ... | 2006 | 16844836 |
effect of salinity on root-nodule conductance to the oxygen diffusion in the cicer arietinum-mesorhizobium ciceri symbiosis. | nodule conductance to o2 diffusion has been involved as a major factor of the inhibition of n2 fixation by soil salinity that severely reduces the production of grain legumes. in order to determine the effect of this constraint on the nodule conductance, oxygen uptake by the nodulated roots of cicer arietinum was measured by recording the concentration of o2 as a function of po2 in a gas-tight incubator. after germination and inoculation with the strain mesorhizobium ciceri upmca7, the varieties ... | 2007 | 16904230 |
characterization of a symbiotically defective serine auxotroph of mesorhizobium ciceri. | a tn5-induced mutant strain (tl68) of mesorhizobium ciceri unable to grow with ammonium as the sole nitrogen source was isolated and characterized. unlike its wild-type parent (strain tal620), the mutant had an absolute dependence on serine to grow. cloning of the dna region containing tn5 and sequence analysis showed that tn5 was inserted into the gene coding for 3-phosphoglycerate dehydrogenase, which catalyses the first step in the serine biosynthetic pathway. the role of serine biosynthesis ... | 2006 | 16978364 |
chickpea (cicer arietinum l.). | chickpea is one of the most important leguminous, cool-season, food crops, cultivated prevalently in the asian pacific region. in spite of its nutritional importance, its area of cultivation has been low, with virtually no increase. conventional breeding has resulted in several important improvements in this crop, and recent advances in biotechnology such as plant tissue culture and genetic transformation can significantly contribute to better sustainability of this important food crop. here, we ... | 2006 | 16988355 |
mapping quantitative trait loci in chickpea associated with time to flowering and resistance to didymella rabiei the causal agent of ascochyta blight. | drought is the major constraint to chickpea (cicer arietinum l.) productivity worldwide. utilizing early-flowering genotypes and advancing sowing from spring to autumn have been suggested as strategies for drought avoidance. however, ascochyta blight (causal agent: didymella rabiei (kov.) v. arx.) is a major limitation for chickpea winter cultivation. most efforts to introgress resistance to the pathogen into kabuli germplasm resulted in relatively late flowering germplasm. with the aim to explo ... | 2006 | 17016689 |
purification and characterization of an n-acetyl-d-galactosamine-specific lectin from seeds of chickpea (cicer arietinum l.). | a novel lectin (caa-ii) was isolated and purified from the seeds of cicer arietinum by ammonium sulphate fractionation and affinity chromatography on an n-acetyl-d-galactosamine-linked agarose column. the lectin is composed of four identical subunits of 30 kda and the molecular mass of the native lectin was estimated to be 120 kda by gel filtration chromatography and confirmed by mass spectrometry. the lectin showed agglutination activity against rabbit erythrocytes (trypsin-treated and untreate ... | 2006 | 17019937 |
alpha-pinene inhibits growth and induces oxidative stress in roots. | determining the mode of action of allelochemicals is one of the challenging aspects in allelopathic studies. recently, allelochemicals have been proposed to cause oxidative stress in target tissue and induce an antioxidant mechanism. alpha-pinene, one of the common monoterpenoids emitted from several aromatic plants including forest trees, is known for its growth-inhibitory activity. however, its mechanism of action remains unexplored. the aim of the present study was to determine the inhibitory ... | 2006 | 17028297 |
effect of zinc supplementation from different sources on growth, nutrient digestibility, blood metabolic profile, and immune response of male guinea pigs. | forty weaned male guinea pigs of 208.20 +/- 6.62 g mean body weight were divided into 4 groups of 10 animals in a randomized block design. all of the guinea pigs were fed a basal diet [25% ground maize hay, 30% ground maize grain, 22% ground chickpea (cicer arietinum l.), 9.5% deoiled rice bran, 6% soybean meal, 6% fish meal, 1.45% mineral supplement (without zn) and 0.05% ascorbic acid] and available green fodder. group i served as the control (no zn supplementation), whereas 20 ppm zn was adde ... | 2006 | 17057264 |
microbiological evaluation of sprouts marketed in mumbai, india, and its suburbs. | a study was undertaken to assess the microbiological quality of sprouts marketed in mumbai and its suburbs. a total of 124 sprout samples of four different legumes--mung (phaseolus aureus), matki (phaseolus aconitifolius), chana (cicer arietinum), and vatana (pisum sativum)--were analyzed over a period of 12 months for aerobic plate counts, coliforms, yeast and mold counts, staphylococci, salmonella, listeria monocytogenes, escherichia coli, e. coli o157:h7, and coagulase-positive staphylococcus ... | 2006 | 17066937 |
the immunolocation of a xyloglucan endotransglucosylase/hydrolase specific to elongating tissues in cicer arietinum suggests a role in the elongation of vascular cells. | in a previous work, a cicer arietinum cdna clone (caxth1) encoding a xyloglucan endotransglucosylase/hydrolase (xth1) protein was isolated and characterized. caxth1 showed an expression pattern specific to growing tissue: mostly epicotyls and the upper growing internodes of adult stems. caxth1 mrna was not detected in any other organs of either seedlings or adult plants, suggesting an involvement of the putative xth encoded by caxth1 in the chickpea cell expansion process. after the generation o ... | 2006 | 17075081 |
osmotic adjustment in chickpea (cicer arietinum l.) results in no yield benefit under terminal drought. | variation in osmotic adjustment (oa) among chickpea (cicer arietinum l.) cultivars has been observed when exposed to terminal drought, but some studies suggest that this benefits yield while others suggest it does not benefit yield in water-limited environments. in the present study, parents differing in oa were crossed and a set of advanced breeding lines (abls) developed for yield testing. the variation in oa during podding was measured under terminal drought in the f(2), f(3), f(7), and f(8) ... | 2007 | 17088363 |
the nuclear proteome of chickpea (cicer arietinum l.) reveals predicted and unexpected proteins. | nuclear proteins constitute a highly organized, complex network that plays diverse roles during cellular development and other physiological processes. the yeast nuclear proteome corresponds to about one-fourth of the total cellular proteins, suggesting the involvement of the nucleus in a number of diverse functions. in an attempt to understand the complexity of plant nuclear proteins, we have developed a proteome reference map of a legume, chickpea, using two-dimensional gel electrophoresis (2- ... | 2006 | 17137331 |
genetic transformation of chickpea (cicer arietinum l.) with insecticidal crystal protein gene using particle gun bombardment. | here, we report the establishment of an efficient particle gun bombardment mediated genetic transformation in chickpea (cicer arietinum l.) using cryiac gene of bacillus thuringiensis. explants were bombarded with recombinant plasmids engineered for the expression of cryiac transgene in plants and stable transformants regenerated in presence of benzyladenine, kinetin and kanamycin. transformation frequency showed dependence on explant type, cultivars, plasmids, helium pressure and microcarrier t ... | 2007 | 17205334 |
identification and characterization of nbs-lrr class resistance gene analogs in faba bean (vicia faba l.) and chickpea (cicer arietinum l.). | a pcr approach with degenerate primers designed from conserved nbs-lrr (nucleotide binding site-leucine-rich repeat) regions of known disease-resistance (r) genes was used to amplify and clone homologous sequences from 5 faba bean (vicia faba) lines and 2 chickpea (cicer arietinum) accessions. sixty-nine sequenced clones showed homologies to various r genes deposited in the genbank database. the presence of internal kinase-2 and kinase-3a motifs in all the sequences isolated confirm that these c ... | 2006 | 17213904 |
evaluation of cymbopogon martinii oil extract for control of postharvest insect deterioration in cereals and legumes. | the essential oil of cymbopogon martinii was tested for its potency as a botanical pesticide to protect stored wheat (triticum aestivum) and gram (garbanzo bean, cicer arietinum) from insect infestation. the c. martinii oil was potent as a fumigant in stored gram. the oil was an effective repellent against the beetles callosobruchus chinensis and tribolium castaneum. geraniol, the major component of the oil, was not as effective as the oil itself. c. martinii oil significantly affected ovipositi ... | 2007 | 17265877 |