| the role of spatial scale and intraspecific variation in secondary chemistry in host-plant location by ceutorhynchus assimilis (coleoptera: curculionidae). | to understand the ecological role of secondary plant compounds in host location by phytophagous insects it is important to consider attraction at different scales in natural populations. the cabbage seed weevil, ceutorhynchus assimilis, which lays eggs in pods of crucifers where the larvae feed on seed, is attracted to purified extracts of specific glucosinolate-derived volatiles. we considered the possibility that c. assimilis adults are attracted to and preferentially attack patches of plants ... | 2001 | 11487403 |
| the exbd2 gene as well as the iron-uptake genes tonb, exbb and exbd1 of xanthomonas campestris pv. campestris are essential for the induction of a hypersensitive response on pepper (capsicum annuum). | the tonb, exbb and exbd1 genes of xanthomonas campestris pv. campestris are essential for ferric iron uptake. in contrast, the exbd2 gene located in the same gene cluster is not essential. mutational analysis revealed that the ferric-iron-uptake genes tonb, exbb and exbd1 are necessary for the induction of a hypersensitive response (hr) on the nonhost plant pepper (capsicum annuum) and the induction of typical black rot symptoms on the host plant cauliflower (brassica oleracea). again, the exbd2 ... | 2000 | 10832632 |
| variation and selection at the cauliflower floral homeotic gene accompanying the evolution of domesticated brassica oleracea. | the evolution of plant morphologies during domestication events provides clues to the origin of crop species and the evolutionary genetics of structural diversification. the cauliflower gene, a floral regulatory locus, has been implicated in the cauliflower phenotype in both arabidopsis thaliana and brassica oleracea. molecular population genetic analysis indicates that alleles carrying a nonsense mutation in exon 5 of the b. oleracea cauliflower (bocal) gene are segregating in both wild and dom ... | 2000 | 10835404 |
| a new method to monitor airborne inoculum of the fungal plant pathogens mycosphaerella brassicicola and botrytis cinerea. | we describe a new microtiter immunospore trapping device (mtist device) that uses a suction system to directly trap air particulates by impaction in microtiter wells. this device can be used for rapid detection and immunoquantification of ascospores of mycosphaerella brassicicola and conidia of botrytis cinerea by an enzyme-linked immunosorbent assay (elisa) under controlled environmental conditions. for ascospores of m. brassicicola correlation coefficients (r(2)) of 0.943 and 0.9514 were obser ... | 2000 | 10877797 |
| comparative mapping of quantitative trait loci sculpting the curd of brassica oleracea. | the enlarged inflorescence (curd) of cauliflower and broccoli provide not only a popular vegetable for human consumption, but also a unique opportunity for scientists who seek to understand the genetic basis of plant growth and development. by the comparison of quantitative trait loci (qtl) maps constructed from three different f(2) populations, we identified a total of 86 qtl that control eight curd-related traits in brassica oleracea. the 86 qtl may reflect allelic variation in as few as 67 di ... | 2000 | 10924486 |
| identification of genetic factors controlling the efficiency of agrobacterium rhizogenes-mediated transformation in brassica oleracea by qtl analysis. | we have identified quantitative trait loci (qtl) for transgenic and adventitious root production using an agrobacterium rhizogenes-mediated co-transformation system in conjunction with a brassica oleracea double haploid (dh) mapping population. three qtl for green fluorescent protein (gfp)-fluorescent root production and four qtl for adventitious root production were identified as accounting for 26% and 32% of the genetic variation in the population, respectively. two of the qtl regions identifi ... | 2002 | 12582506 |
| host-correlated morphological variation of myzus persicae (hemiptera: aphididae) populations in greece. | morphological variation in nine characters of 157 clones of myzus persicae (sulzer) was examined by multivariate analysis. the clones were collected from peach, prunus persica, the primary host and the secondary hosts tobacco, nicotiana tabacum, cabbage, brassica oleracea, sugarbeet, beta vulgaris and pepper capsicum annuum. the 156 clones originated from various regions of greece, both in the north, where a large part of the population has an annual bisexual generation on peach, and in more sou ... | 2000 | 10996864 |
| a comparison of peronospora parasitica (downy mildew) isolates from arabidopsis thaliana and brassica oleracea using amplified fragment length polymorphism and internal transcribed spacer 1 sequence analyses. | amplified fragment length polymorphism (aflp) fingerprints and internal transcribed spacer 1 (its1) sequences from 27 peronospora parasitica isolates (collected from arabidopsis thaliana or brassica oleracea), 5 albugo candida isolates (from the same hosts and from capsella bursa-pastoris), and 1 bremia lactucae isolate (from lactuca sativa) were compared. the aflp analysis divided the isolates into five groups that correlated with taxonomic species and, in most cases, with host origin. the only ... | 2000 | 11017765 |
| nitrate leaching in an andisol treated with different types of fertilizers. | nitrate (no3) leaching was studied in an andisol treated with four n fertilizers (sc: swine compost, cu: coated urea, an: ammonium n, or nf: no fertilizer) for 7 years. sweet corn (zea mays l.) was grown in summer, followed by chinese cabbage (brassica rapa l. var. amplexicaulis) or cabbage (brassica oleracea l. var. capitata) in autumn each year. in chemical fertilizer plots treated with an or cu, no(3)-n concentrations in soil water at 1-m depth increased markedly in the summer of the second y ... | 2003 | 12685774 |
| comparison of the expression patterns of two small gene families of s gene family receptor kinase genes during the defence response in brassica oleracea and arabidopsis thaliana. | sfr2, a member of the s gene family of receptor kinases, has been shown to be rapidly induced by wounding and bacterial infection suggesting that this gene may play a role in the defence response in brassica. in this study we have compared the response of sfr2 to that of two other members of the sfr gene family in brassica (sfr1 and sfr3) and to the closely-related ark genes of arabidopsis. different patterns of mrna accumulation were observed for different members of these families. sfr1 transc ... | 2002 | 11814694 |
| antitrypanosomal effect of the aqueous extract of brassica oleracea. | the in vitro antitrypanosomal activity of the aqueous extract of brassica oleracea, was investigated in trypanosoma brucei brucei 'lafia' strain. the extract was found to be effective by immobilizing the trypanosomes within the 3-h incubation period and thereafter rendering them not infective to mice. | 2002 | 11864758 |
| a mixed green vegetable and fruit beverage decreased the serum level of low-density lipoprotein cholesterol in hypercholesterolemic patients. | the effects of a canned mixed green vegetable and fruit beverage, containing broccoli (brassica oleracea l. var. botrytis l.) and cabbage (brassica oleracea l. var. capitata l.) as main materials, on serum lipid levels in hypercholesterolemic patients were investigated. thirty-one adult subjects were administered two cans of the beverage (160 g/can) per day for 3 weeks. their serum total cholesterol (tc) levels significantly decreased from 6.7 +/- 0.8 to 6.1 +/- 0.6 mmol/l, and, more strikingly, ... | 2002 | 12010009 |
| effect of cabbage cultivars with varying levels of resistance to aphids on the performance of the parasitoid, aphidius colemani (hymenoptera: braconidae). | the effect of aphid resistance in plant cultivars on parasitism of myzus persicae (sulzer) and brevicoryne brassicae (linnaeus) by aphidius colemani viereck was investigated under laboratory conditions using three cultivars of common cabbage, brassica oleracea var. capitata. significantly greater aphid populations were found on cv. derby day (green-leaved, susceptible to both aphid species), regardless of the presence or absence of parasitoids, compared with cv. minicole (green-leaved, partially ... | 2002 | 12020362 |
| antifungal activity of brassica oleracea var. botrytis fresh aqueous juice. | the antifungal activity of fresh, aqueous brassica oleracea var. botrytis juice against candida albicans and other pathogenic fungi was investigated. the juice was found to be effective both in inhibiting the growth of blastoconidia and reducing the appearance of c. albicans germ tubes. furthermore, the juice inhibited the growth of some pathogenic, filamentous fungi. | 2003 | 12837360 |
| loss of function of the fusarium oxysporum snf1 gene reduces virulence on cabbage and arabidopsis. | fusarium oxysporum pathogenicity is believed to require the activity of cell wall-degrading enzymes. production of these enzymes in fungi is subject to carbon catabolite repression, a process that in yeast is mostly controlled by the snf1 (sucrose non-fermenting 1) gene. to elucidate the role of cell wall-degrading enzymes in f. oxysporum pathogenicity, we cloned and disrupted its snf1 homologue ( fosnf1). the fosnf1 mutants had a reduced expression of several genes encoding cell wall-degrading ... | 2003 | 12845476 |
| genetic transformation of cauliflower (brassica oleracea var. botrytis) by direct dna uptake into mesophyll protoplasts. | mesophyll protoplasts of brassica oleracea var. botrytis were successfully transformed using polyethylene glycol (peg). the success of plant transformation depended on both gene transfer and plant regeneration. parameters, such as peg and vector concentrations and heat shock conditions were tested in experiments on transient expression of the beta-glucuronidase (ec 3.2.1.31) gene and the most suitable conditions for dna uptake were determined. two antibiotic resistance marker genes for neomycin ... | 2002 | 12060266 |
| kale carotenoids remain stable while flavor compounds respond to changes in sulfur fertility. | dietary intake of certain carotenoids has been associated with a reduced risk of disease. kale (brassica oleracea l. acephala group) has the highest levels of carotenoids lutein and beta-carotene, and is an excellent source of minerals among the green leafy vegetable crops. however, brassica vegetables contain glucosinolate (gs) and s-methylcysteine sulfoxide (mcso). while these sulfur compounds have medicinal value, they are also responsible for the bitter, acrid flavors that are often regarded ... | 2003 | 12926877 |
| [contribution to the study of the biological value of calcium; broccoli leaves-brassica oleracea, l. var.: botrytis asparagoides. i..]. | | 1953 | 13134734 |
| [biological value of calcium broccoli leaves: brassica oleracea, l. var.: botrytis asparagoides. ii..]. | | 1953 | 13134749 |
| [biological value of calcium: broccoli leaves, brassica oleracea, l. var.: botrytis asparagoides. iii]. | | 1953 | 13155997 |
| [preliminary findings on the endocrine changes in rabbits fed cabbage (brassica oleracea) & rape (brassica campestris oleifera)]. | | 1957 | 13490561 |
| effect of an extract of cauliflower (leaf) on the labeling of blood elements with technetium-99m and on the survival of escherichia coli ab1157 submitted to the treatment with stannous chloride. | the labeling of red blood cells (rbc) with technetium-99m (99mtc) depends on a reducing agent and stannous chloride (sncl(2)) and is widely utilized. this labeling may also be altered by drugs, and sncl(2) reduces the survival of escherichia coli cultures. cauliflower (brassica oleracea l. var. botrytis) is used in folk medicine and we evaluated its influence on (i) the labeling of blood elements with 99mtc, and (ii) on the survival of an e. coli strain. blood was withdrawn from rats that drank ... | 2002 | 12065213 |
| eurobionet: a pan-european biomonitoring network for urban air quality assessment. | eurobionet, the 'european network for the assessment of air quality by the use of bioindicator plants', is an eu-funded cooperative project currently consisting of public authorities and scientific institutes from 12 cities in 8 countries. in 2000, the bioindicator plants tobacco (nicotiana tabacum bel w3), poplar (populus nigra 'brandaris'), spiderwort (tradescantia sp. clone 4430), italian rye grass (lolium multiflorum italicum) and curly kale (brassica oleracea acephala) were exposed to ambie ... | 2002 | 12094534 |
| a thapsigargin-sensitive ca(2+) pump is present in the pea golgi apparatus membrane. | the golgi apparatus behaves as a bona fide ca(2+) store in animal cells and yeast (saccharomyces cerevisiae); however, it is not known whether this organelle plays a similar role in plant cells. in this work, we investigated the presence of an active ca(2+) accumulation mechanism in the plant cell golgi apparatus. toward this end, we measured ca(2+) uptake in subcellular fractions isolated from the elongating zone of etiolated pea (pisum sativum) epicotyls. separation of organelles using sucrose ... | 2002 | 12177495 |
| purification and transcriptional analysis of rna polymerase i holoenzymes from broccoli (brassica oleracea) and frog (xenopus laevis). | | 2003 | 14712639 |
| internal transcribed spacer 2 amplicon as a molecular marker for identification of peronospora parasitica (crucifer downy mildew). | the purpose of the study was to characterize the internal transcribed spacer (its) regions of peronospora parasitica (crucifer downy mildew) in order to evaluate their potential as molecular markers for pathogen identification. | 2004 | 14962138 |
| inhibition of phospholipase d alpha by n-acylethanolamines. | n-acylethanolamines (naes) are endogenous lipids in plants produced from the phospholipid precursor, n-acylphosphatidylethanolamine, by phospholipase d (pld). here, we show that seven types of plant naes differing in acyl chain length and degree of unsaturation were potent inhibitors of the well-characterized, plant-specific isoform of pld-pld alpha. it is notable that pld alpha, unlike other pld isoforms, has been shown not to catalyze the formation of naes from n-acylphosphatidylethanolamine. ... | 2002 | 12177503 |
| chicken model for studying dietary antioxidants reveals that apple (cox's orange)/broccoli (brassica oleracea l. var. italica) stabilizes erythrocytes and reduces oxidation of insoluble muscle proteins and lipids in cooked liver. | a chicken model for studying the effects of antioxidants in the diet on oxidative status was set up. chickens fed a semi-synthetic diet low in antioxidants showed a remarkable decrease in erythrocyte stability toward h(2)o(2) or 2,2'-azobis (2-amidinopropane) dihydrochloride (aaph), but increases in catalase activity in liver, carbonyls in insoluble muscle proteins, and enhanced lipid oxidation in heat-treated liver samples compared to that of conventionally fed chickens. thus, this chicken mode ... | 2002 | 12188608 |
| zinc requirement for stomatal opening in cauliflower. | zn deficiency induced increases in epicuticular wax deposits, lamina thickness, degree of succulence, water saturation deficit, diffusive resistance, and proline accumulation and decreases in carbonic anhydrase activity, water potential, stomatal aperture, and transpiration in the leaves of cauliflower (brassica oleracea l. var botrytis cv pusa) plants. restoration of zn supply to the deficient plants increased stomatal aperture, transpiration, and carbonic anhydrase activity significantly withi ... | 1995 | 12228399 |
| microsomal lyso-phosphatidic acid acyltransferase from a brassica oleracea cultivar incorporates erucic acid into the sn-2 position of seed triacylglycerols. | developing seeds from brassica oleracea (l.) var botrytis cv sesam were examined for the ability to biosynthesize and incorporate erucic acid into triacylglycerols (tags). seed embryos at mid-development contained a high concentration of erucic acid in diacylglycerols and tags, and substantial levels were also detected in free fatty acids, acyl-coenzyme a (coa), phosphatidic acid, and phosphatidylcholine. homogenates and microsomal fractions from seeds at mid-development produced [14c]eicosenoyl ... | 1995 | 12228602 |
| modification of phospholipid catabolism in microsomal membranes of [gamma]-irradiated cauliflower (brassica oleracea l.). | acceleration of membrane deterioration has been observed recently during storage of [gamma]-irradiated cauliflower (brassica oleracea l., botrytis group). in the present study, the activity of microsome-associated lipolytic enzymes was investigated in cauliflower florets exposed to 0 or 4 kilograys of [gamma] radiation and stored for 8 d at 13[deg]c. radiolabeled breakdown products obtained from the metabolism of (16:0/18:2*)-phosphatidylcholine and (16:0/16:0)-phosphatidyl-[n-methyl-3h]choline ... | 1993 | 12231812 |
| elemental sulphur as an induced antifungal substance in plant defence. | man's oldest fungicide has probably long functioned in this role in plants, as a natural component of induced antifungal defence. elemental sulphur (s(0)) is the only inorganic phytoalexin and the only phytoalexin produced by so many different taxa. s(0) (detected by gc-ms as (32)s(8)) is produced in representative species of sterculiaceae (cocoa), solanaceae (tomato, tobacco), malvaceae (cotton), and leguminosae (french bean) in response to xylem-invading fungal and bacterial pathogens. product ... | 2004 | 15181110 |
| immunopurification and immunocharacterization of the glucosinolate biosynthetic enzyme thiohydroximate s-glucosyltransferase. | preparing homogeneous udp-glucose:thiohydroximate s-glucosyltransferase (s-gt), the penultimate biosynthetic enzyme of glucosinolates, by standard chromatographic methods has yielded too little protein for adequate purity evaluation, identity verification, and structural analysis. the low yields were apparently due to low abundance in source tissues, aggravated by enzyme instability. here we describe an immunological method for purification of workable quantities from florets of brassica olerace ... | 1994 | 12232212 |
| an important determinant of the ability of turnip mosaic virus to infect brassica spp. and/or raphanus sativus is in its p3 protein. | turnip mosaic virus (tumv, genus potyvirus, family potyviridae) infects mainly cruciferous plants. isolates tu-3 and tu-2r1 of tumv exhibit different infection phenotypes in cabbage (brassica oleracea l.) and japanese radish (raphanus sativus l.). infectious full-length cdna clones, ptuc and ptur1, were constructed from isolates tu-3 and tu-2r1, respectively. progeny virus derived from infections with ptuc induced systemic chlorotic and ringspot symptoms in infected cabbage, but no systemic infe ... | 2004 | 15218194 |
| rhizoctonia wilt suppression of brinjal (solanum melongena l) and plant growth activity by bacillus bs2. | an antibiotic-producing and hydrogen-cyanide-producing rhizobacteria strain bacillus bs2 showed a wide range of antifungal activity against many fusarium sp. and brinjal wilt disease pathogen rhizoctonia solani. seed bacterization with the strain bs2 promoted seed germination and plant growth in leguminous plants phaseolus vulgaris and non-leguminous plants solanum melongena l, brassica oleracea var. capitata, b. oleraceae var. gongylodes and lycopersicon esculentum mill in terms of relative gro ... | 2003 | 15266911 |
| ban103, a pollen-preferential gene, from chinese cabbage and its promoter activity. | we isolated and characterized a pollen-preferential gene, ban103, from chinese cabbage and analyzed the activity of its promoter. the ban103 cdna and genomic clone that contained the full-length gene were sequenced. the ban103 gene is a single copy in the chinese cabbage genome, and divided into three exons by two introns. the deduced sequence of 68 amino acids showed a homology with the brassica oleracea pollen coat protein, as well as several cold-induced proteins. ban103 transcription was res ... | 2002 | 12243345 |
| mapping of a locus for adult plant resistance to downy mildew in broccoli (brassica oleracea convar. italica). | the identification of the gene pp523, conferring downy mildew resistance to adult plants of broccoli (brassica oleracea convar. italica), led to the construction of a genetic map that included this resistance locus, 301 amplified fragment length polymorphisms, 55 random amplified polymorphic dnas, 46 inter-simple sequence repeats, three simple sequence repeats, four other pcr markers and a flower colour locus, all gathered into nine major linkage groups. nineteen additional molecular markers wer ... | 2004 | 15278283 |
| a brassica self-incompatibility gene is expressed in the stylar transmitting tissue of transgenic tobacco. | tobacco was transformed with a gene coding for an s-locus-specific glycoprotein of brassica oleracea. the resulting transgenic plants showed tissue-specific and developmentally regulated expression of the introduced gene. immunolocalization experiments showed that the brassica gene was expressed in the stylar transmitting tissue of the transgenic plants. the pattern of expression of the introduced gene was more similar to that of the s-associated genes of nicotiana alata than to expression in br ... | 1990 | 12354943 |
| brassica s-proteins accumulate in the intercellular matrix along the path of pollen tubes in transgenic tobacco pistils. | a tobacco plant transformed with a brassica oleracea slg-22 gene was analyzed by immunocytochemical methods to determine the localization of the transgene-encoded protein product. immunolabeling was observed in the pistil along the path followed by pollen tubes after pollination. s-antigen accumulated in the intercellular matrix of the transmitting tissue of the style and its continuation in the basal portion of the stigma and outside a few special cells of the placental epidermis of the ovary. ... | 1990 | 12354944 |
| identifying sources and mechanisms of resistance in crucifers for control of cabbage maggot (diptera: anthomyiidae). | the cabbage maggot, delia radicum (l.) is an important insect pest of eruciferous crops in upstate new york. this species causes considerable damage to seedlings and young plants by feeding on roots and stems, resulting in plant stand loss and yield loss. five crucifer accessions (brassica oleracea variety italica l.,'green comet'; b. oleracea l.,'rapid cycling' [crucifer genetics cooperative 3-1 ]; b. oleracea variety botrytis l., a standard cauliflower cultivar'amazing'; b. carinata l.; and si ... | 2001 | 11561856 |
| quantitative determination of 13c-labeled and endogenous beta-carotene, lutein, and vitamin a in human plasma. | quantitative procedures employing liquid-chromatography/particle beam-mass spectrometry (lc/pb-ms) and gas chromatography-mass spectrometry (gc-ms) were applied to the determination of the endogenous and 13c-labeled beta-carotene, lutein, and retinol in plasma of a subject who consumed kale (brassica oleracea) that had been grown in a 13co2-enriched atmosphere. all compounds were analyzed in the negative chemical ionization (nci) mode using methane as the moderating reagent gas. beta-carotene an ... | 2001 | 11795861 |
| highly informative nature of inter simple sequence repeat (issr) sequences amplified using tri- and tetra-nucleotide primers from dna of cauliflower (brassica oleracea var. botrytis l.). | inter simple sequence repeat (issr) sequences as molecular markers can lead to the detection of polymorphism and also be a new approach to the study of ssr distribution and frequency. in this study, issr amplification with nonanchored primer was performed in closely related cauliflower lines. fourty-four different amplified fragments were sequenced. sequences of pcr products are delimited by the expected motifs and number of repeats, which validates the issr nonanchored primer amplification tech ... | 2002 | 12416621 |
| restoring enzyme activity in nonfunctional low erucic acid brassica napus fatty acid elongase 1 by a single amino acid substitution. | genomic fatty acid elongation 1 (fae1) clones from high erucic acid (hea) brassica napus, brassica rapa and brassica oleracea, and low erucic acid (lea) b. napus cv. westar, were amplified by pcr and expressed in yeast cells under the control of the strong galactose-inducible promoter. as expected, yeast cells expressing the fae1 genes from hea brassica spp. synthesized very long chain monounsaturated fatty acids that are not normally found in yeast, while fatty acid profiles of yeast cells expr ... | 2002 | 12423362 |
| a new member of plant cs-lyases. a cystine lyase from arabidopsis thaliana. | cystine lyases catalyze the breakdown of l-cystine to thiocysteine, pyruvate, and ammonia. until now there are no reports of the identification of a plant cystine lyase at a molecular level, and it is not clear what biological role this class of enzymes have in plants. a cystine lyase was isolated from brassica oleracea (l.), and partial amino acid sequencing allowed the corresponding full-length cdna (bocl3) to be cloned. the deduced amino acid sequence of bocl3 showed highest homology to the d ... | 2003 | 12525491 |
| purification of a plant nucleotide pyrophosphatase as a protein that interferes with nitrate reductase and glutamine synthetase assays. | an activity that inhibited both glutamine synthetase (gs) and nitrate reductase (nr) was highly purified from cauliflower (brassica oleracea var. botrytis) extracts. the final preparation contained an acyl-coa oxidase and a second protein of the plant nucleotide pyrophosphatase family. this preparation hydrolysed nadh, atp and fad to generate amp and was inhibited by fluoride, cu2+, zn2+ and ni2+. the purified fraction had no effect on the activity of nr when reduced methylviologen was used as e ... | 2003 | 12631294 |
| valorization of cauliflower (brassica oleracea l. var. botrytis) by-products as a source of antioxidant phenolics. | the present study reports the development of two extraction protocols, with potential industrial applicability, to valorize cauliflower (brassica oleracea l. var. botrytis) byproducts as a source of antioxidant phenolics. in addition, the nonionic polystyrene resin amberlite xad-2 was used to obtain purified extracts. the extract yield, phenolic content, phenolic yield, and correlation between the antioxidant activity and the phenolic content were studied. the water and ethanol protocols yield a ... | 2003 | 12670153 |
| hplc-dad-ms/ms esi characterization of unusual highly glycosylated acylated flavonoids from cauliflower (brassica oleracea l. var. botrytis) agroindustrial byproducts. | in a recent program to investigate the possible use of vegetable handling and commercialization wastes and residues as a source of potentially bioactive extracts, we have studied cauliflower byproducts. a total of twenty-eight compounds were characterized by hplc-dad-ms, of these, twenty-two are produced naturally by the plant. the main compounds found were kaempferol 3-diglucoside-7-glucoside and its combinations with different hydroxycinnamic acids. moreover, some flavonoids with an unusual hi ... | 2003 | 12797762 |
| hymenopteran parasitoids of diamondback moth (lepidoptera: ypeunomutidae) in northern thailand. | larvae of the diamondback moth, plutella xylostella (l.) (lepidoptera: ypeunomutidae), cause severe economic damage to cabbage, brassica oleracea l. variety capitata (brassicaceae) and related vegetables in thailand. overuse of broad-spectrum insecticides for diamondback moth control is a serious problem and has obscured the contributions of indigenous parasitoids. our objectives were to identify indigenous diamondback moth parasitoids in northern thailand and to assess their potential for natur ... | 2005 | 15889737 |
| recombinant water-soluble chlorophyll protein from brassica oleracea var. botrys binds various chlorophyll derivatives. | a gene coding for water-soluble chlorophyll-binding protein (wscp) from brassica oleracea var. botrys has been used to express the protein, extended by a hexahistidyl tag, in escherichia coli. the protein has been refolded in vitro to study its pigment binding behavior. recombinant wscp was found to bind two chlorophylls (chls) per tetrameric protein complex but no carotenoids in accordance with previous observations with the native protein [satoh, h., nakayama, k., okada, m. (1998) j. biol. che ... | 2003 | 12809498 |
| n-linked glycosylation of native and recombinant cauliflower xyloglucan endotransglycosylase 16a. | the gene encoding a xet (xyloglucan endotransglycosylase) from cauliflower ( brassica oleracea var. botrytis ) florets has been cloned and sequenced. sequence analysis indicated a high degree of similarity to other xet enzymes belonging to glycosyl hydrolase family 16 (gh16). in addition to the conserved gh16 catalytic sequence motif eidfe, there exists one potential n-linked glycosylation site, which is also highly conserved in xet enzymes from this family. purification of the corresponding pro ... | 2003 | 12826015 |
| molecular mapping of or, a gene inducing beta-carotene accumulation in cauliflower (brassica oleracea l. var. botrytis). | the cauliflower (brassica oleracea l. var. botrytis) or gene is a semi-dominant, single-locus mutation that induces the accumulation of high levels of beta-carotene in various tissues of the plant, turning them orange. as part of a map-based cloning strategy, molecular mapping of the or gene in the cauliflower genome was undertaken in a mapping population consisting of 195 f2 individuals. by using amplified fragment length polymorphism (aflp) in conjunction with bulked segregant analysis, we ide ... | 2003 | 12897867 |
| genetic analysis of agrobacterium tumefaciens susceptibility in brassica oleracea. | the genetic control and heritability of agrobacterium tumefaciens susceptibility was investigated using a doubled haploid (dh) mapping population of brassica oleracea and the associated rflp map. preliminary studies were carried out by analysis of an 8 x 8 diallel, for which the parental lines were selected to include a range of susceptibilities to a. tumefaciens. the variation observed within the diallel was attributed to both additive and dominant gene effects, with additive gene effects being ... | 2004 | 14534750 |
| interaction of calmodulin, a sorting nexin and kinase-associated protein phosphatase with the brassica oleracea s locus receptor kinase. | recognition of self-pollen during the self-incompatibility response in brassica oleracea is mediated by the binding of a secreted peptide (the s locus cysteine-rich protein) to the s locus receptor kinase (srk), a member of the plant receptor kinase (prk) superfamily. here, we describe the characterization of three proteins that interact with the cytosolic kinase domain of srk. a b. oleracea homolog of arabidopsis kinase-associated protein phosphatase was shown to interact with and dephosphoryla ... | 2003 | 14555783 |
| genes and environment interact to determine the fitness costs of resistance to bacillus thuringiensis. | genes which provide resistance to novel challenges such as pesticides, toxins or pathogens often impose fitness costs on individuals with a resistant phenotype. studies of resistance to bacillus thuringiensis and its insecticidal cry toxins indicate that fitness costs may be variable and cryptic. using two field populations (karak and serd4) of the diamondback moth, plutella xylostella, we tested the hypothesis that the costs associated with resistance to the b. thuringiensis toxin cry1ac would ... | 2005 | 16011928 |
| polioencephalomalacia in cattle in new zealand fed chou moellier (brassica oleracea). | polioencephalomalacia was diagnosed clinically in 26 heifers from a herd of 99 grazing chou moellier (brassica oleracea). clinical signs included ataxia, recumbency, blindness and aimless walking. characteristic polioencephalomalacic autofluorescence was seen under long ultraviolet radiation of four fresh brains examined in the veterinary clinic and two formalin-fixed brains sectioned and examined in the laboratory. haemorrhage and malacia were noted grossly and microscopically in the cranial co ... | 1997 | 16031945 |
| [regulation to curd morphogenesis and genetics of exogenous bocal gene in cauliflower]. | cauliflower (brassica oleracea var. botrytis l.) and cabbage (b. oleracea var capitata l.) are two varieties of species b. oleracea. cauliflower bobcal gene losses the original function due to its terminated mutation while cabbage bocal gene has the complete function. we obtained cauliflower plants transgenic for bocal gene using arobacterium-mediated transformation. genetic analysis of t2 generation indicated that all cauliflower plants transgenic for bocal failed to form curd and produced the ... | 2003 | 14574987 |
| effects of soil amendments on lead uptake by two vegetable crops from a lead-contaminated soil from anhui, china. | previous studies have documented that phosphate compounds of lead (pb) [e.g., pyromorphite pb5(po4)3-(x), where x=oh, f or cl] are comparatively insoluble, and their formation in pb-contaminated soil may be a means of reducing the bioavailability and chemical lability of pb in soil. in this study, the effect of phosphate compound amendments on the bioavailability of pb in a polluted alkaline soil was examined. a pb-contaminated soil was treated with hydroxyapatite (ha), phosphate rock (pr), wate ... | 2004 | 14987865 |
| a dynamic binomial sequential sampling plan for plutella xylostella (lepidoptera: plutellidae) on broccoli and cauliflower in australia. | binomial sequential sampling plans have been used widely for monitoring invertebrate pest populations. such plans are typically based upon a single action threshold (at), which represents the level of infestation that the grower is prepared to accept before using a control measure. for many cropping systems this acceptable infestation level is likely to vary, being dependent on factors such as the growth stage of the crop and the value or demands of the destination market (e.g., local or high-qu ... | 2004 | 14998136 |
| isolate-specific and broad-spectrum qtls are involved in the control of clubroot in brassica oleracea. | clubroot, caused by plasmodiophora brassicae, is one of the most damaging diseases of vegetable brassica crops in the world. in this study, genetic control and mapping of loci implied in quantitative resistance against five isolates of p. brassicae were studied in the f(1) and f(2/3 )progenies of the cross c10 (resistant kale)xhdem (susceptible broccoli). a genetic map was constructed using rflp, random and specific pcr-based markers. the 199 loci were assembled into nine linkage groups covering ... | 2004 | 15007504 |
| genotypic effects on glucosinolates and sensory properties of broccoli and cauliflower. | the objective of this study was to investigate the influence that the species, form or cultivar exerts on the glucosinolate spectrum for glucosinolates and free sugars in selected vegetable species of the brassica genus (brassica oleracea var. italica plenck, brassica rapa var. alboglabra and brassica oleracea var. botrytis l.). the results showed significant differences amongst the cultivar groups for the glucosinolate proportions as well as the contents of health-promoting and flavour-influenc ... | 2004 | 15053347 |
| phytotoxicity of cobalt, chromium and copper in cauliflower. | cauliflower (brassica oleracea l. var. botrytis cv. maghi) was grown in refined sand with complete nutrition (control) and at 0.5 mm each of co, cr and cu. in cauliflower, compared to that of excess cu or cr, the visible effects of excess co appeared first and were most pronounced. excess of each heavy metal restricted the biomass of cauliflower, concentrations of fe, chlorophylls a and b, protein and activity of catalase in leaves in the order co>cu>cr. the translocation of cr from roots to top ... | 2000 | 15092914 |
| the use of phenotypic markers to identify brassica oleracea genotypes for routine high-throughput agrobacterium-mediated transformation. | doubled haploid (dh) genotypes from a genetic mapping population of brassica oleracea were screened for ease of transformation. candidate genotypes were selected based on prior knowledge of three phenotypic markers: susceptibility to agrobacterium tumefaciens, shoot regeneration potential and mode of shoot regeneration. mode of regeneration was found to be the most significant of the three factors. transgenic plants were successfully obtained from genotypes that regenerated multiple shoots via a ... | 2004 | 15197481 |
| behaviour of a recombinant cabbage (brassica oleracea) phospholipase d immobilized on cnbr-activated and antibody supports. | recombinant cabbage (brassica oleracea) pld2 (phospholipase d2) immobilized covalently on cnbr-activated sepharose expressed low activity (approximately 10%), while that immobilized by binding on to anti-pld2 igg-sepharose was more active (approximately 38%). coupling of pld2 to cnbr-activated sepharose resulted in significant improvement in storage stability without affecting its thermostability, as compared with the soluble enzyme. binding of pld2 to the antibody support, however, rendered the ... | 2004 | 15270712 |
| emission of plutella xylostella-induced compounds from cabbages grown at elevated co2 and orientation behavior of the natural enemies. | several plant species defend themselves indirectly from herbivores by producing herbivore-induced volatile compounds that attract the natural enemies of herbivores. here we tested the effects of elevated atmospheric co(2) (720 micromol mol(-1)) concentration on this indirect defense, physiological properties, and constitutive and induced emissions of white cabbage (brassica oleracea ssp. capitata, cvs lennox and rinda). we monitored the orientation behavior of the generalist predator podisus mac ... | 2004 | 15299116 |
| [transformation of bt-cpti fusion protein gene to cabbage (brassica oleracea var. capitata) mediated by agrobacterium tumefaciens and particle bombardment]. | bt-cpti fusion protein gene was transferred to the explants of hypocotyl, cotyledon with petiole and shoot apex of cabbage (brassica oleracea l. var. capitata) variety "zhonggan no 8" via agrobacterium tumefaciens and particle bombardment, and 13 kanamycin-resistant plants were obtained. pcr and southern blotting hybridization verified that all these plants of the kanr plants of type i regenerated from hypocotyl and cotyledon with petiole mediated by a. tumefaciens were transgenic plants, 2 karr ... | 2002 | 15344329 |
| laboratory evaluations of a wild crucifer barbarea vulgaris as a management tool for the diamondback moth plutella xylostella (lepidoptera: plutellidae). | the term 'dead-end trap cropping' has recently been proposed to identify a plant that is highly attractive for oviposition by an insect pest, but on which offspring of the pest cannot survive. the potential of the wild crucifer barbarea vulgaris r. br. to allure and serve as a dead-end trap crop for the diamondback moth plutella xylostella (l.), an important pest of cruciferous crops worldwide, was examined in laboratory experiments. when p. xylostella adults were provided with a dual-choice of ... | 2004 | 15541190 |
| the chromoplasts of or mutants of cauliflower (brassica oleracea l. var. botrytis). | the or mutation in cauliflower (brassica oleracea l. var. botrytis) leads to abnormal accumulations of beta-carotene in orange chromoplasts, in tissues in which leucoplasts are characteristic of wild-type plants. or chromoplasts were investigated by light microscopy of fresh materials and electron microscopy of glutaraldehyde- and potassium permanganate-fixed materials. carotenoid inclusions in or chromoplasts resemble those found in carrot root chromoplasts in their optical activity and angular ... | 2004 | 15614485 |
| traditional phytotherapy in central italy (marche, abruzzo, and latium). | in this study, the more significant results of extensive ethnopharmacobotanical research carried out by the author in the years 1977-2000 in 175 localities of three regions of central italy (marche, abruzzo, and latium) have been reported and compared. the usages of 80 species belonging to 36 families are described, of which 71 were used in human therapy and 29 in veterinary medicine. uses are suited with the number of localities in which they have been mentioned. among the wild plant mainly sti ... | 2005 | 15664457 |
| the respiratory chain components of higher plant mitochondria. | tightly coupled mitochondria have been prepared from a variety of plant sources: white potato (solanum tuberosum), jerusalem artichoke (heliantus tuberosus), cauliflower buds (brassica oleracea), and mung bean hypocotyls (phaseolus aureus). mitochondria with no appreciable coupling were also prepared from skunk cabbage spadices (symplocarpus foetidus).room temperature difference spectra show that these mitochondria are very similar in the qualitative and quantitative composition of their electro ... | 1968 | 16656837 |
| screening and evaluation of resistance to downy mildew (peronospora parasitica) and clubroot (plasmodiophora brassicae) in genetic resources of brassica oleracea. | 52 entries including landraces, old cultivars and wild accessions of b. oleracea and closely related brassica species were screened for resistance against downy mildew and clubroot. several accessions resistant to downy mildew and a few to clubroot were found. genetic inheritance of the resistance in downy mildew was investigated by screening f1 and bc1f1 offspring from three resistant landrace accessions crossed with both a resistant and a susceptible father. the seedling resistance against dow ... | 2004 | 15703046 |
| the application of bioassays as indicators of petroleum-contaminated soil remediation. | bioremediation has proven successful in numerous applications to petroleum contaminated soils. however, questions remain as to the efficiency of bioremediation in lowering long-term soil toxicity. in the present study, the bioassays spirotox, microtox, ostracodtoxkit f, umu-test with s-9 activation, and plant assays were applied, and compared to evaluate bioremediation processes in heavily petroleum contaminated soils. six higher plant species (secale cereale l., lactuca sativa l., zea mays l., ... | 2005 | 15722101 |
| regulation of succinate dehyrogenase in higher plants: i. some general characteristics of the membrane-bound enzyme. | the spectrophotometric phenazine methosulfate assay of succinate dehydrogenase was adapted to use with cauliflower (brassica oleracea) and mung bean (phaseolus aureus) mitochondria with suitable modifications to overcome the permeability barrier to the dye. procedures in the literature for the isolation and sonic disruption of mitochondria from these sources were modified to assure maximal yield and stability of the enzyme. in tightly coupled mung bean mitochondria, as isolated, about half of th ... | 1973 | 16658617 |
| regulation of succinate dehydrogenase in higher plants: ii. activation by substrates, reduced coenzyme q, nucleotides, and anions. | the effect of various agents on the activation of succinate dehydrogenase in cauliflower (brassica oleracea) and mung bean (phaseolus aureus) mitochondria and in sonicated particles has been investigated. reduced coenzyme q(10), inosine diphosphate, inosine triphosphate, acid ph, and anions activate the enzyme in mitochondria from higher plants in the same manner as in mammalian preparations. significant differences have been detected in the behavior of plant and animal preparations in the effec ... | 1973 | 16658618 |
| differences between adenosine triphosphatases from monocotylous and dicotylous plants. | the mitochondrial membrane-bound atpases of several plants were investigated. two distinct types were encountered. the mitochondrial atpases of castor bean (ricinus communis var. zanzibarensis, l.), cauliflower (brassica oleracea, l.), and scarlet runner (phaseolus coccineus, l.) were found to be inhibited by oligomycin, to have elevated molecular weights when separated from the organelles by ultrasonication and ammonium sulfate treatment and, subsequent to purification, to be cold-labile. on th ... | 1977 | 16659806 |
| vitreoscilla hemoglobin overexpression increases submergence tolerance in cabbage. | agrobacterium tumefaciens was used to deliver the vhb gene into cabbage (brassica oleracea var. cabitata) cv. xiaguang's parent line, 103. using hypocotyls and cotyledon petioles as explants for infection, we obtained a transformation efficiency of 3-5% based on the number of transgenic shoots produced from the number of explants used for infection. molecular analysis indicated that the vhb gene was stably integrated into the cabbage genome and that the vhb gene was expressed at the rna level. c ... | 2004 | 15747159 |
| transgene expression in broccoli (brassica oleracea var. italica) clones propagated in vitro via leaf explants. | we have developed an efficient protocol for the in vitro propagation of transgenic broccoli plants using leaf explants as starting material. a high frequency of shoot formation from leaf explants was obtained on murashige and skoog medium containing benzyladenine (ba, 5 mg/l) and naphthaleneacetic acid (0.5 mg/l). frequent subcultures of existing shoots and shoot clusters to medium containing only ba (2 mg/l) promoted rapid shoot multiplication. the use of a 1:1 mixture of agargel and gelrite in ... | 2003 | 12789524 |
| variation in plant volatiles and attraction of the parasitoid diadegma semiclausum (hellén). | differences in allelochemistry of plants may influence their ability to attract parasitoids. we studied responses of diadegma semiclausum (hellén), a parasitoid of the diamondback moth (plutella xylostella l.), to inter- and intraspecific variation in odor blends of crucifers and a non-crucifer species. uninfested brussels sprout (brassica oleracea l. gemmifera), white mustard (sinapis alba l.), a feral brassica oleracea, and malting barley (hordeum vulgare l.) were compared for their attractivi ... | 2005 | 15898495 |
| mechanisms of action and antiproliferative properties of brassica oleracea juice in human breast cancer cell lines. | cruciferous vegetables are an important source of compounds that may be useful for chemoprevention. in this study, we evaluated the antiproliferative activity of juice obtained from leaves of several varieties of brassica oleracea on both estrogen receptor (er)-positive (er+; mcf-7 and bt474) and er-negative (er-; mda-mb-231 and bt20) human breast cancer cell lines. the effect of juice on cell proliferation was evaluated on dna synthesis and on cell cycle-related proteins. juice markedly reduced ... | 2005 | 15930460 |
| qtls controlling the production of transgenic and adventitious roots in brassica oleracea following treatment with agrobacterium rhizogenes. | brassica oleracea can be genetically engineered using agrobacterium rhizogenes. the initial stage of this process is the production of transgenic ('hairy') roots; shoots are subsequently regenerated from these roots. previous work using gus and gfp reporter genes has shown that genotypes of b. oleracea vary in their performance for transgenic root production. quantitative trait loci (qtls) controlling this trait have been located in one mapping population. the current study provides evidence tha ... | 2005 | 15942754 |
| broccoli and watercress suppress matrix metalloproteinase-9 activity and invasiveness of human mda-mb-231 breast cancer cells. | a high dietary intake of cruciferous vegetables has been associated with a reduction in numerous human pathologies particularly cancer. in the current study, we examined the inhibitory effects of broccoli (brassica oleracea var. italica) and watercress (rorripa nasturtium aquaticum) extracts on 12-o-tetradecanoylphorbol-13-acetate (tpa)-induced cancer cell invasion and matrix metalloproteinase-9 activity using human mda-mb-231 breast cancer cells. aberrant overexpression of matrix metalloprotein ... | 2005 | 15953625 |
| suppression of the cysteine protease, aleurain, delays floret senescence in brassica oleracea. | an aleurain-like protein, bocp5, is up-regulated during harvest-induced senescence in broccoli floret and leaf tissue. bocp5 is most closely related to an arabidopsis protein (91%, aaf43041) and has 71% identity to barley aleurain (p05167). the mrna for this gene accumulates within 6 h after harvest in broccoli florets, and its expression is reduced in tissue that has been held in senescence-delaying treatments (e.g. water, sucrose feeding, controlled atmosphere). the gene is also expressed in l ... | 2005 | 15988561 |
| variation in the coat protein sequence of british isolates of turnip yellow mosaic virus and comparison with previously published isolates. brief report. | isolates of turnip yellow mosaic virus (tymv) were collected from wild cabbage (brassica oleracea) on a 400 m stretch of dorset coastline. the coat protein genes of four isolates showed high homology in nucleotide sequence (0.970-1.000, mean 0.987). lower levels of homology where found to previously published sequences of australian isolates [10] (0.725-0.775, mean 0.741). the amino acid composition of the dorset isolates showed high levels of homology (0.964-1.000, mean 0.986). numerous amino a ... | 2005 | 16052285 |
| [cloning of acc oxidase gene and inhibition of endogenous gene expression with rnai in cauliflower]. | a fragment of 1202 bp of the candidate aco gene was amplified from the cauliflower (brassica oleracea var. botrytis) genome using the degenerated primers which were designed according to the consensus sequence of aco amino acids among various plant species. the result of blast showed the sequence presented a very high match with the aco genes from other plants; the homologue was from 83% to 99%. three exons and two introns were identified in this sequence. the spliced length of mrna was 756 nt a ... | 2005 | 16078747 |
| [the study of transformation of tobacco with the stress responsible gene bors1 from brassica oleracea var. acephala]. | transgenic tobacco plants expressing a stress responsive gene bors1, isolated from brassica oleracea var. acephala, under the control of the 35s promoter of the cauliflower mosaic virus were produced. some plants were further used to test the effect of high level bors1 expression on drought stress resistance. the presence of transgene in putative transgenic plants was confirmed by pcr analysis. thirty-six among 130 transformants showed amplification of predicted fragment of bors1 while no amplif ... | 2005 | 16108381 |
| importance of direct spray and spray residue contact for infection of trichoplusia ni larvae by field applications of beauveria bassiana. | commercial formulations and unformulated conidia of beauveria bassiana strain gha were applied to field-grown plants and artificially infested with trichoplusia ni (hübner) larvae to compare the relative insecticidal activity resulting from direct spray contact with insecticidal activity due to contact with dry spray residue. in general, applications to cabbage, brassica oleracea l., resulted in nearly equal mortalities when comparing insects exposed to direct spray contact with those exposed by ... | 2006 | 16937663 |
| antibiotic uptake by plants from soil fertilized with animal manure. | antibiotics are commonly added to animal feed as supplements to promote growth of food animals. however, absorption of antibiotics in the animal gut is not complete and as a result substantial amounts of antibiotics are excreted in urine and feces that end up in manure. manure is used worldwide not only as a source of plant nutrients but also as a source of organic matter to improve soil quality especially in organic and sustainable agriculture. greenhouse studies were conducted to determine whe ... | 2005 | 16221828 |
| are glucocerebrosides the predominant sphingolipids in plant plasma membranes? | long-chain sphingobases have been analyzed in various fractions prepared from different organs (leaf, root, storage tissue) from five dicotyledoneous plants (arabidopsis thaliana, brassica oleracea, nicotiana tabacum, pisum sativum, spinacia oleracea). the resulting sphingobase profiles from cerebrosides and plasma membranes (pms) show large qualitative and quantitative differences. assuming that cerebrosides from all cellular membranes have similar sphingobase profiles, these data suggest that ... | 2005 | 16386430 |
| activity of brassica oleracea leaf juice on foodborne pathogenic bacteria. | many vegetables of the cruciferae family have been found to possess antimicrobial properties against several microorganisms of clinical importance. in this study, we reported the antibacterial effect of brassica oleracea juice on several food-borne pathogens. the juice was found to be effective in inhibiting the growth of salmonella enteritidis, verotoxigenic escherichia coli o157:h7, e. coli hb producing thermolabile toxin, nontoxigenic e. coli, and listeria monocytogenes, but not enterococcus ... | 2006 | 16995537 |
| transgenic tobacco plants expressing bors1 gene from brassica oleracea var. acephala show enhanced tolerance to water stress. | water stress is by far the leading environmental stress limiting crop yields worldwide. genetic engineering techniques hold great promise for developing crop cultivars with high tolerance to water stress. in this study, the brassica oleracea var. acephala bors1 gene was transferred into tobacco through agrobacterium-mediated leaf disc transformation. the transgenic status and transgene expression of the transgenic plants was confirmed by polymerase chain reaction (pcr) analysis, southern hybridi ... | 2005 | 16388139 |
| kale carotenoids are unaffected by, whereas biomass production, elemental concentrations, and selenium accumulation respond to, changes in selenium fertility. | selenium (se) is a micronutrient in mammalian nutrition and is accumulated in kale (brassica oleracea l. var. acephala), which has high levels of lutein and beta-carotene. selenium, lutein, and beta-carotene have important human health benefits and possess strong antioxidant properties. the objectives of this study were to determine the influence of different se [as sodium selenate (na(2)seo(4)) and sodium selenite (na(2)seo(3))] fertility levels on (1) biomass accumulation, (2) the accumulation ... | 2006 | 16506831 |
| brassica oleracea pollen, a new source of occupational allergens. | vegetable pollen is a rare source of occupational allergens. occupational allergy has only been described in the case of paprika pollen and tomato pollen. we describe a new source of occupational pollen allergy. | 2006 | 16512813 |
| epithiospecifier protein from broccoli (brassica oleracea l. ssp. italica) inhibits formation of the anticancer agent sulforaphane. | in some cruciferous plants, epithiospecifier protein (esp) directs myrosinase (ec 3.2.3.1)-catalyzed hydrolysis of alkenyl glucosinolates toward epithionitrile formation. here, for the first time, we show that esp activity is negatively correlated with the extent of formation of the health-promoting phytochemical sulforaphane in broccoli (brassica oleracea l. ssp. italica). a 43 kda protein with esp activity and sequence homology to the esp of arabidopsis thaliana was cloned from the broccoli cv ... | 2006 | 16536577 |
| evaluation of a chemically inducible promoter for developing a within-plant refuge for resistance management. | chemically inducible production of bacillus thuringiensis (bt) toxins in transgenic plants may provide considerable benefits in preventing or delaying the evolution of insect resistance to bt crops by creating within-plant temporal refuges. we examined the effect of inducible cry1ab expression on survival of different genotypes (rr, rs, and ss) of diamondback moth, plutella xylostella (l.), in transgenic broccoli, brassica oleracea l., plants transformed with a pr-1a/cry1ab expression cassette. ... | 2005 | 16539150 |
| identification of pathogenesis-related ests in the crucifer downy mildew oomycete hyaloperonospora parasitica by high-throughput differential display analysis of distinct phenotypic interactions with brassica oleracea. | crucifer downy mildew is caused by the obligatory biotrophic oomycete hyaloperonospora parasitica (formerly peronospora parasitica). so far, isolates infecting arabidopsis thaliana have proven to be non-pathogenic on other crucifers and, despite its unequivocal merit as a research model, the pathosystem a. thaliana-h. parasitica by itself will not provide all the answers onto crucifer downy mildew genetics and biology. in this report, we present the development of a differential display (dd)-bas ... | 2006 | 16545472 |
| cultivation of garden vegetables in peoria pool sediments from the illinois river: a case study in trace element accumulation and dietary exposures. | this case study was conducted to evaluate the use of reclaimed lake sediment as a growth media for vegetable production and to estimate whether accumulation of micronutrients and heavy metals in the vegetables would impact human nutrition or health, respectively. five plant species, bean (phaseolus vulgaris l.), broccoli (brassica oleracea l.), carrot (daucus carota l.), pepper (capsicum annum l.), and tomato (lycopersicon esculentum l.), were grown in pots containing either reclaimed sediment f ... | 2006 | 16650471 |
| serine transhydroxymethylase of cauliflower (brassica oleracea var. botrytis l.): partial purification and properties. | serine transhydroxymethylase (ec 2.1.2.1) has been purified 46-fold from cauliflower (brassica oleracea var. botrytis l.). the enzyme was completely dependent on the presence of tetrahydrofolic acid for the conversion of serine to glycine. the addition of pyridoxal phosphate gave a large increase in the reaction rate. a double ph optimum was observed with maxima at 7.5 and 9.5. the enzyme is specific for l-serine. the d-isomer is neither a substrate nor an inhibitor. the michaelis constants for ... | 1967 | 16656717 |
| the association of carbohydrate changes in the shoot tip of cauliflower with flowering. | changes in levels of sugars and starch in the shoot tip of cauliflower, brassica oleracea l. var. botrytis d. c. cv. main crop were studied during periods of growth which were inductive or non-inductive to flowering. flowering was induced by growing plants for 2 weeks under 16 hr of light at 5 degrees . during this period of floral induction there was a significant increase in sugar and starch content compared to that in vegetative plants grown at 20 to 26 degrees . sugar and starch content did ... | 1968 | 16656956 |
| an evaluation of 4-s-methyl-2-keto-butyric acid as an intermediate in the biosynthesis of ethylene. | stimulation of ethylene production by cauliflower (brassica oleracea var. botrytis l.) tissue in buffer solution containing 4-s-methyl-2-keto-butyric acid is not due to activation of the natural in vivo system. increased ethylene production derives from an extra-cellular ethylene-forming system, catalyzed by peroxidase and other factors, which leak from the cauliflower tissue and cause the degradation of 4-s-methyl-2-keto-butyric acid. this exogenous ethylene-forming system is similar to the eth ... | 1971 | 16657663 |
| regulation of plant pyruvate dehydrogenase complex by phosphorylation. | the atp-dependent inactivation of the pyruvate dehydrogenase complex (pdc) was examined using ruptured mitochondria and partially purified pyruvate dehydrogenase complex isolated from broccoli and cauliflower (brassica oleracea) bud mitochondria. the atp-dependent inactivation was temperature- and ph-dependent. [(32)p]atp experiments show a specific transphosphorylation of the gamma-po(4) of atp to the complex. the phosphate attached to the pdc was labile under mild alkaline but not under mild a ... | 1977 | 16660037 |
| [identification of pcr markers associated with cytoplasmic male sterility in brassica oleracea var botrytis]. | the homology-based candidate gene method was used to identified the specific pcr markers linked to cytoplasmic male sterility (cms) in cauliflower( brassica oleracea var botrytis.). searching the dna and protein data-base of ncbi , correlative genes or open reading frames were identified . analysis of biosoft, based on the conservative regions ,five primers were designed . among them, only primer p9/p10 produced a 313- bp specific fragment. identified by individual plant testing , analysis of rt ... | 2005 | 15843352 |