| analysis of s-locus and expression of s-alleles of self-compatible rapid-cycling brassica oleracea 'to1000dh3'. | brassica oleracea is a strictly self-incompatible (si) plant, but rapid-cycling b. oleracea 'to1000dh3' is self-compatible (sc). self-incompatibility in brassicaceae is controlled by multiple alleles of the s-locus. three s-locus genes, s-locus glycoprotein (slg), s-locus receptor kinase (srk) and s-locus protein 11 or s-locus cysteine-rich (sp11/scr), have been reported to date, all of which are classified into class i and ii. in this study, we investigated the molecular mechanism behind altera ... | 2014 | 24969488 |
| antioxidant responses of annelids, brassicaceae and fabaceae to pollutants: a review. | pollutants, such as metal trace elements (mtes) and organic compounds (polycyclic aromatic hydrocarbons, pesticides), can impact dna structure of living organisms and thus generate damage. for instance, cadmium is a well-known genotoxic and mechanisms explaining its clastogenicity are mainly indirect: inhibition of dna repair mechanisms and/or induction of reactive oxygen species (ros). animal or vegetal cells use antioxidant defense systems to protect themselves against ros produced during oxid ... | 2015 | 24951273 |
| the complete mitochondrial genome sequence of brassica oleracea and analysis of coexisting mitotypes. | the complete mitochondrial genome sequences of brassica species have provided insight into inter- and intraspecific variation of plant mitochondrial genomes. however, the size of mitochondrial genome sequenced for brassica oleracea hitherto does not match to its physical mapping data. this fact led us to investigate b. oleracea mitochondrial genome in detail. here we report novel b. oleracea mitochondrial genome, derived from var. capitata, a cabbage cultivar ''fujiwase''. the genome was assembl ... | 2014 | 24916859 |
| effect of water content and temperature on inactivation kinetics of myrosinase in broccoli (brassica oleracea var. italica). | broccoli belongs to the brassicaceae plant family consisting of widely eaten vegetables containing high concentrations of glucosinolates. enzymatic hydrolysis of glucosinolates by endogenous myrosinase (myr) can form isothiocyanates with health-promoting activities. the effect of water content (wc) and temperature on myr inactivation in broccoli was investigated. broccoli was freeze dried obtaining batches with wc between 10% and 90% (aw from 0.10 to 0.96). these samples were incubated for vario ... | 2014 | 24912716 |
| comparative analysis of 11 brassicales mitochondrial genomes and the mitochondrial transcriptome of brassica oleracea. | to elucidate the evolution of mitochondrial genomic diversity within a single order of angiosperms, we sequenced seven brassicales genomes and the transcriptome of brassica oleracea. in the common ancestor of brassicaceae, several genes of known function were lost and the ccmfn gene was split into two independent genes, which also coincides with a trend of genome reduction towards the smallest sequenced angiosperm genomes of brassica. for most orfs of unknown function, the lack of conservation t ... | 2014 | 24907441 |
| genome-wide analysis of the distribution of ap2/erf transcription factors reveals duplication and cbfs genes elucidate their potential function in brassica oleracea. | cabbage (brassica oleracea) is one of the most important leaf vegetables grown worldwide. the entire cabbage genome sequence and more than fifty thousand proteins have been obtained to date. however a high degree of sequence similarity and conserved genome structure remain between cabbage and arabidopsis; therefore, arabidopsis is a viable reference species for comparative genomics studies. transcription factors (tfs) are important regulators involved in plant development and physiological proce ... | 2014 | 24888752 |
| purification of peroxidase from red cabbage (brassica oleracea var. capitata f. rubra) by affinity chromatography. | peroxidase was purified in a single step using 4-amino benzohydrazide affinity chromatography from red cabbage (brassica oleracea var. capitata f. rubra), and some important biochemical characteristics of the purified enzyme were determined. the enzyme, with a specific activity of 3,550 eu/mg protein, was purified 120.6-fold with a yield of 2.9% from the synthesized affinity matrix. the molecular weight of the enzyme was found to be 69.3 kda by sodium dodecyl sulfate-polyacrylamide gel electroph ... | 2014 | 24879596 |
| evaluation of different cooking conditions on broccoli (brassica oleracea var. italica) to improve the nutritional value and consumer acceptance. | the objective of this study was to gain insights into the effect of the cooking method on the liking as well as the retention of glucosinolates in broccoli. with this knowledge it can be concluded whether the health aspects of broccoli be improved by the cooking method without deteriorating sensory perception. for this, broccoli was cooked by methods commonly applied by consumers: boiling with a cold (water) start; boiling with a hot (water) start; and steaming. firmness, greenness and amount of ... | 2014 | 24853375 |
| the brassica oleracea genome reveals the asymmetrical evolution of polyploid genomes. | polyploidization has provided much genetic variation for plant adaptive evolution, but the mechanisms by which the molecular evolution of polyploid genomes establishes genetic architecture underlying species differentiation are unclear. brassica is an ideal model to increase knowledge of polyploid evolution. here we describe a draft genome sequence of brassica oleracea, comparing it with that of its sister species b. rapa to reveal numerous chromosome rearrangements and asymmetrical gene loss in ... | 2014 | 24852848 |
| ultrasonic-assisted enzymatic extraction of phenolics from broccoli (brassica oleracea l. var. italica) inflorescences and evaluation of antioxidant activity in vitro. | an efficient ultrasonic-assisted enzymatic extraction technique was applied to extracting phenolics from broccoli inflorescences without organic solvents. the synergistic model of enzymolysis and ultrasonication simultaneously was selected, and the enzyme combination was optimized by orthogonal test: cellulase 7.5 mg/g fw (fresh weight), pectinase 10 mg/g fw, and papain 1.0 mg/g fw. the operating parameters in ultrasonic-assisted enzymatic extraction were optimized with response surface methodol ... | 2015 | 24837595 |
| will chemical defenses become more effective against specialist herbivores under elevated co2? | elevated atmospheric co2 is known to affect plant-insect herbivore interactions. elevated co2 causes leaf nitrogen to decrease, the ostensible cause of herbivore compensatory feeding. co2 may also affect herbivore consumption by altering chemical defenses via changes in plant hormones. we considered the effects of elevated co2, in conjunction with soil fertility and damage (simulated herbivory), on glucosinolate concentrations of mustard (brassica nigra) and collard (b. oleracea var. acephala) a ... | 2014 | 24832554 |
| the impact of using alternative forages on the nutrient value within slurry and its implications for forage productivity in agricultural systems. | alternative forages can be used to provide valuable home-grown feed for ruminant livestock. utilising these different forages could affect the manure value and the implications of incorporating these forages into farming systems, needs to be better understood. an experiment tested the hypothesis that applying slurries from ruminants, fed ensiled red clover (trifolium pratense), lucerne (medicago sativa) or kale (brassica oleracea) would improve the yield of hybrid ryegrass (lolium hybridicum), c ... | 2014 | 24830777 |
| heterologous expression of atmyb12 in kale (brassica oleracea var. acephala) leads to high flavonol accumulation. | overexpression of arabidopsis atmyb12 transcription factor greatly increases the total phenolic and flavonol content in transgenic kale leaves. flavonoids are a diverse group of plant secondary metabolites exhibiting a number of health-promoting effects. there has been a growing interest to develop biotechnological methods for the enhanced production of flavonoids in crop plants. atmyb12 is an arabidopsis transcription factor which specifically activates flavonol synthesis and its overexpression ... | 2014 | 24792422 |
| ultrasound assisted immersion freezing of broccoli (brassica oleracea l. var. botrytis l.). | the aim of this study was to research the ultrasound-assisted freezing (uaf) of broccoli. | 2014 | 24746509 |
| rice-straw mulch reduces the green peach aphid, myzus persicae (hemiptera: aphididae) populations on kale, brassica oleracea var. acephala (brassicaceae) plants. | organic mulches, like peel and rice-straw, besides other materials affect the uv and temperature, which cause a reduction in the aphid arrival. the aim was to evaluate the effect of covering the soil with straw on the populations of the green peach aphid, myzus persicae on the kale, brassica oleracea var. acephala plants. the first experiment evaluated the direct effect of the rice-straw mulch and the second its indirect effect on aphid immigration, testing the plant characteristics that could l ... | 2014 | 24714367 |
| insecticide residues in head lettuce, cabbage, chinese cabbage, and broccoli grown in fields. | the residues of four insecticides belonging to different families were studied on head lettuce (lactuca sativa l. var. capitata l.), cabbage (brassica oleracea linn. var. capitata dc.), chinese cabbage (brassica pekinensis skeels), and broccoli (brassica oleracea var. italica) after pesticide application. to reduce application variability, a tank mix of acetamiprid 20% sp, chlorpyrifos 22.5% ec, deltamethrin 2.4% sc, and methomyl 40% sp was applied at recommended and double doses. initial deposi ... | 2014 | 24684565 |
| extracts from brassica oleracea l. convar. acephala var. sabellica inhibit tnf-α stimulated neutrophil adhesion in vitro under flow conditions. | the beneficial effects of vegetables such as leafy cabbage (brassica oleracea) on health are attributed to their anti-oxidative and anti-inflammatory potential. therefore, we investigated whether curly kale extracts affect cytokine induced expression of endothelial cell adhesion molecules as well as the adhesion of leukocytes to endothelial cells depending on their polyphenol content and composition. curly kale leaves were extracted applying two solvents with different polarities (methanolic ext ... | 2014 | 24683602 |
| high-throughput sequencing and de novo assembly of brassica oleracea var. capitata l. for transcriptome analysis. | the cabbage, brassica oleracea var. capitata l., has a distinguishable phenotype within the genus brassica. despite the economic and genetic importance of cabbage, there is little genomic data for cabbage, and most studies of brassica are focused on other species or other b. oleracea subspecies. the lack of genomic data for cabbage, a non-model organism, hinders research on its molecular biology. hence, the construction of reliable transcriptomic data based on high-throughput sequencing technolo ... | 2014 | 24682075 |
| combined alkaline hydrolysis and ultrasound-assisted extraction for the release of nonextractable phenolics from cauliflower (brassica oleracea var. botrytis) waste. | cauliflower waste contains high amounts phenolic compounds, but conventional solvent extraction misses high amounts of nonextractable phenolics (nep), which may contribute more to the valorization of these waste streams. in this study, the nep content and composition of cauliflower waste were investigated. the ability of alkaline hydrolysis, sonication, and their combination to release nep was assessed. alkaline hydrolysis with sonication was found to extract the highest nep content (7.3 ± 0.17 ... | 2014 | 24665894 |
| interaction of moderate uv-b exposure and temperature on the formation of structurally different flavonol glycosides and hydroxycinnamic acid derivatives in kale (brassica oleracea var. sabellica). | kale has a high number of structurally different flavonol glycosides and hydroxycinnamic acid derivatives. in this study we investigated the interaction of moderate uv-b radiation and temperature on these compounds. kale plants were grown at daily mean temperatures of 5 or 15 °c and were exposed to five subsequent daily doses (each 0.25 kj m(-2) d(-1)) of moderate uv-b radiation at 1 d intervals. of 20 phenolic compounds, 11 were influenced by an interaction of uv-b radiation and temperature, e. ... | 2014 | 24655223 |
| evaluation of genetic diversity in chinese kale (brassica oleracea l. var. alboglabra bailey) by using rapid amplified polymorphic dna and sequence-related amplified polymorphism markers. | chinese kale is an original chinese vegetable of the cruciferae family. to select suitable parents for hybrid breeding, we thoroughly analyzed the genetic diversity of chinese kale. random amplified polymorphic dna (rapd) and sequence-related amplified polymorphism (srap) molecular markers were used to evaluate the genetic diversity across 21 chinese kale accessions from avrdc and guangzhou in china. a total of 104 bands were detected by 11 rapd primers, of which 66 (63.5%) were polymorphic, and ... | 2014 | 24615113 |
| identification of metabolic qtls and candidate genes for glucosinolate synthesis in brassica oleracea leaves, seeds and flower buds. | glucosinolates are major secondary metabolites found in the brassicaceae family. these compounds play an essential role in plant defense against biotic and abiotic stresses, but more interestingly they have beneficial effects on human health. we performed a genetic analysis in order to identify the genome regions regulating glucosinolates biosynthesis in a dh mapping population of brassica oleracea. in order to obtain a general overview of regulation in the whole plant, analyses were performed i ... | 2014 | 24614913 |
| plastid transformation in cabbage (brassica oleracea l. var. capitata l.) by the biolistic process. | cabbage (brassica oleracea l. var. capitata l.) is one of the most important vegetable crops grown worldwide. scientists are using biotechnology in addition to traditional breeding methods to develop new cabbage varieties with desirable traits. recent biotechnological advances in chloroplast transformation technology have opened new avenues for crop improvement. in 2007, we developed a stable plastid transformation system for cabbage and reported the successful transformation of the cry1ab gene ... | 2014 | 24599866 |
| a metabolomics approach to identify factors influencing glucosinolate thermal degradation rates in brassica vegetables. | thermal processing of brassica vegetables can lead to substantial loss of potential health-promoting glucosinolates (gls). the extent of thermal degradation of a specific gl varies in different vegetables, possibly due to differences in the composition of other metabolites within the plant matrices. an untargeted metabolomics approach followed by random forest regression was applied to identify metabolites associated to thermal gl degradation in a segregating brassica oleracea population. out of ... | 2014 | 24594187 |
| exposure factors for wastewater-irrigated asian vegetables and a probabilistic rotavirus disease burden model for their consumption. | many farmers in water-scarce regions of developing countries use wastewater to irrigate vegetables and other agricultural crops, a practice that may expand with climate change. there are a number of health risks associated with wastewater irrigation for human food crops, particularly with surface irrigation techniques common in the developing world. the world health organization (who) recommends using quantitative microbial risk assessment (qmra) to determine if the irrigation scheme meets healt ... | 2014 | 24576153 |
| noncrop flowering plants restore top-down herbivore control in agricultural fields. | herbivore populations are regulated by bottom-up control through food availability and quality and by top-down control through natural enemies. intensive agricultural monocultures provide abundant food to specialized herbivores and at the same time negatively impact natural enemies because monocultures are depauperate in carbohydrate food sources required by many natural enemies. as a consequence, herbivores are released from both types of control. diversifying intensive cropping systems with fl ... | 2013 | 24567828 |
| identification of suitable qpcr reference genes in leaves of brassica oleracea under abiotic stresses. | real-time quantitative pcr is nowadays a standard method to study gene expression variations in various samples and experimental conditions. however, to interpret results accurately, data normalization with appropriate reference genes appears to be crucial. the present study describes the identification and the validation of suitable reference genes in brassica oleracea leaves. expression stability of eight candidates was tested following drought and cold abiotic stresses by using three differen ... | 2014 | 24566730 |
| transcriptome sequencing of two parental lines of cabbage (brassica oleracea l. var. capitata l.) and construction of an est-based genetic map. | expressed sequence tag (est)-based markers are preferred because they reflect transcribed portions of the genome. we report the development of simple sequence repeat (ssr) and single nucleotide polymorphism (snp) markers derived from transcriptome sequences in cabbage, and their utility for map construction. | 2014 | 24559437 |
| chicken manure enhanced yield and quality of field-grown kale and collard greens. | organic matter and nutrients in municipal sewage sludge (ss) and chicken manure (cm) could be recycled and used for land farming to enhance fertility and physical properties of soils. three soil management practices were used at kentucky state university research farm, franklin county, to study the impact of soil amendments on kale (brassica oleracea cv. winterbar) and collard (brassica oleracea cv. top bunch) yields and quality. the three soil management practices were: (i) ss mixed with native ... | 2014 | 24502217 |
| comparative analysis of alternative splicing, alternative polyadenylation and the expression of the two kin genes from cytoplasmic male sterility cabbage (brassica oleracea l. var. capitata l.). | the kin genes are crucial members of the cold-regulated gene family. they play exclusive roles during the developmental processes of many organs and respond to various abiotic stresses in plants. however, little is known about the regulation of kin gene expression in cytoplasmic male sterility (cms) cabbages (brassica oleracea l. var. capitata l.). we carried out a genome-wide analysis to identify the kin genes in the cms cabbage. two non-redundant kin genes, named bokin1 (bol021262) and bokin2 ... | 2014 | 24488150 |
| self-(in)compatibility inheritance and allele-specific marker development in yellow mustard (sinapis alba). | yellow mustard (sinapis alba) has a sporophytic self-incompatibility reproduction system. genetically stable self-incompatible (si) and self-compatible (sc) inbred lines have recently been developed in this crop. understanding the s haplotype of different inbred lines and the inheritance of the self-(in)compatibility (si/sc) trait is very important for breeding purposes. in this study, we used the s-locus gene-specific primers in brassica rapa and brassica oleracea to clone yellow mustard s-locu ... | 2014 | 24482603 |
| red cabbage (brassica oleracea l.) mediates redox-sensitive amelioration of dyslipidemia and hepatic injury induced by exogenous cholesterol administration. | the widely used culinary vegetable, red cabbage (brassica oleracea l. var. capitata f. rubra), of the brassicaceae family contains biologically potent anthocyanins and a myriad of antioxidants. previous studies have shown that the pharmacological effects of red cabbage in vivo are redox-sensitive. the present study explored whether red cabbage modulates various histopathological and biochemical parameters in rats administered with a cholesterol-rich diet (crd). to this end, prolonged administrat ... | 2014 | 24467544 |
| simultaneous determination of sulphoraphane and sulphoraphane nitrile in brassica vegetables using ultra-performance liquid chromatography with tandem mass spectrometry. | several analytical methods exist for the determination of sulphoraphane or sulphoraphane nitrile from biological matrices and plant extracts. however, no uplc-ms/ms method exists for the simultaneous detection of both. | 2016 | 24449540 |
| upregulation of the at-hook dna binding gene bomf2 in ogucms anthers of brassica oleracea suggests that it encodes a transcriptional regulatory factor for anther development. | ogura cytoplasmic male sterility (ogucms) is the most important cms system used for f1 hybrid cabbage production worldwide. the anther abortion and defective pollen development exhibited in ogucms are coordinately regulated by the mitochondrial male sterile gene orf138 and many nuclear transcriptional regulatory factors. at-hook dna binding proteins regulate cell-specific gene expression. in this study, we cloned the gene encoding the at-hook dna binding protein bomf2 using the cdna-aflp tdf seq ... | 2014 | 24443226 |
| effect of post harvest radiation processing and storage on the volatile oil composition and glucosinolate profile of cabbage. | effect of radiation processing (0.5-2 kgy) and storage on the volatile oil constituents and glucosinolate profile of cabbage was investigated. among the volatile oil constituents, an enhancement in trans-hex-2-enal was noted on irradiation that was attributed to the increased liberation of precursor linolenic acid mainly from monogalactosyl diacyl glycerol (mgdg). irradiation also enhanced sinigrin, the major glucosinolate of cabbage that accounted for the enhanced allyl isothiocyanate (aitc) in ... | 2014 | 24423497 |
| the c-terminal extension peptide of non-photoconvertible water-soluble chlorophyll-binding proteins (class ii wscps) affects their solubility and stability: comparative analyses of the biochemical and chlorophyll-binding properties of recombinant brassica, raphanus and lepidium wscps with or without their c-terminal extension peptides. | numerous members of the brassicaceae possess non-photoconvertible water-soluble chlorophyll (chl)-binding proteins (class ii wscps), which function as chl scavengers during cell disruption caused by wounding, pest/pathogen attacks, and/or environmental stress. class ii wscps have two extension peptides, one at the n-terminus and one at the c-terminus. the n-terminal peptide acts as a signal peptide, targeting the protein to the endoplasmic reticulum body, a unique defensive organelle found only ... | 2014 | 24389799 |
| diversification times among brassica (brassicaceae) crops suggest hybrid formation after 20 million years of divergence. | cruciferous vegetables, many of which are in the genus brassica (brassicaceae), are prized for their nutritive value and have been cultivated for thousands of years. there are numerous wild northwestern mediterranean species in the tribe brassiceae, and it is therefore assumed this center of diversity is also the region of origin. within the tribe, the nigra and oleracea clades contain the three diploid brassica crops, b. oleracea, b. rapa, and b. nigra. these three species hybridized in the pas ... | 2014 | 24388963 |
| cabbage (brassica oleracea l. var. capitata) phytochemicals with antioxidant and anti-inflammatory potential. | the objective of this study was to investigate antioxidant and anti-inflammatory activity of cabbage phytochemicals. | 2014 | 24377584 |
| bottom-up and top-down herbivore regulation mediated by glucosinolates in brassica oleracea var. acephala. | quantitative differences in plant defence metabolites, such as glucosinolates, may directly affect herbivore preference and performance, and indirectly affect natural enemy pressure. by assessing insect abundance and leaf damage rate, we studied the responses of insect herbivores to six genotypes of brassica oleracea var. acephala, selected from the same cultivar for having high or low foliar content of sinigrin, glucoiberin and glucobrassicin. we also investigated whether the natural parasitism ... | 2014 | 24352843 |
| microrna319a-targeted brassica rapa ssp. pekinensis tcp genes modulate head shape in chinese cabbage by differential cell division arrest in leaf regions. | leafy heads of cabbage (brassica oleracea), chinese cabbage (brassica rapa), and lettuce (lactuca sativa) are composed of extremely incurved leaves. the shape of these heads often dictates the quality, and thus the commercial value, of these crops. using quantitative trait locus mapping of head traits within a population of 150 recombinant inbred lines of chinese cabbage, we investigated the relationship between expression levels of microrna-targeted brassica rapa ssp. pekinensis teosinte branch ... | 2014 | 24351684 |
| the importance of aboveground-belowground interactions on the evolution and maintenance of variation in plant defense traits. | over the past two decades a growing body of empirical research has shown that many ecological processes are mediated by a complex array of indirect interactions occurring between rhizosphere-inhabiting organisms and those found on aboveground plant parts. aboveground-belowground studies have thus far focused on elucidating processes and underlying mechanisms that mediate the behavior and performance of invertebrates in opposite ecosystem compartments. less is known about genetic variation in pla ... | 2013 | 24348484 |
| optimization of methyl jasmonate application to broccoli florets to enhance health-promoting phytochemical content. | spray treatment of methyl jasmonate (meja) has been shown to increase glucosinolate (gs) concentrations and health-promoting activity in brassica vegetables. since there is no reported standardized protocol, several meja treatment studies have been conducted to maximize human health bioactivity using the f1 broccoli cultivar 'green magic'. | 2014 | 24338840 |
| seed germination, root elongation, root-tip mitosis, and micronucleus induction of five crop plants exposed to chromium in fluvo-aquic soil. | the present study aimed to determine the toxic effects of chromium (cr) on cabbage (brassica oleracea), cucumber (cucumis sativus), lettuce (lactuca sativa), wheat (triticum aestivum), and corn (zea mays), and identify the sensitive plant species and appropriate bioassays for potential use in phytotoxicity assessment of cr in soil. results showed that seed germination might not be a sensitive assay for assessing cr toxicity because at most of the cr levels there were no toxic effects. root elong ... | 2014 | 24318542 |
| proline partially overcomes excess molybdenum toxicity in cabbage seedlings grown in vitro. | in vitro grown cabbage (brassica oleracea var. capitata) seedlings exposed to excess molybdenum (mo) ions exhibited severely reduced plant growth at the cotyledonary stage. adding 80 mm proline (pro) to the mo-treated medium could help 50% seedlings to overcome the toxicity and grow true leaves. under excess mo stress, seedlings accumulated blue/purple anthocyanin in their cotyledons and hypocotyls. the anthocyanin content under mo with 40 mm pro was 4-fold higher than the control medium, ms wit ... | 2013 | 24301928 |
| retraction notice to "two novel bioactive glucosinolates from broccoli (brassica oleracea l. var. italica) florets" [bioorg. med. chem. lett. 22 (2012) 5555-5558]. | | 2013 | 24288810 |
| negative regulation of abscisic acid signaling by the brassica oleracea abi1 ortholog. | abi1 (aba insensitive 1) is an important component of the core regulatory network in early aba (abscisic acid) signaling. here, we investigated the functions of an abi1 ortholog in brassica oleracea (bolabi1). the expression of bolabi1 was dramatically induced by drought, and constitutive expression of bolabi1 confers aba insensitivity upon the wild-type. subcellular localization and phosphatase assays reveal that bolabi1 is predominantly localized in the nucleus and harbors phosphatase activity ... | 2013 | 24269821 |
| bolost1, an ortholog of open stomata 1 with alternative splicing products in brassica oleracea, positively modulates drought responses in plants. | open stomata 1 (ost1), an aba-activated sucrose non-fermenting 1 (snf1)-related protein kinase, is critical for plant drought responses. we investigated the functions of two splicing isoforms of the ost1 ortholog in brassica oleracea (bolost1). bolost1 expression was found to be dramatically induced by drought and high-salt stress, and the ectopic expression of bolost1 restored the drought-sensitive phenotype of ost1. subcellular localization revealed that bolost1 is localized in both the nucleu ... | 2013 | 24269232 |
| testing local host adaptation and phenotypic plasticity in a herbivore when alternative related host plants occur sympatrically. | host race formation in phytophagous insects can be an early stage of adaptive speciation. however, the evolution of phenotypic plasticity in host use is another possible outcome. using a reciprocal transplant experiment we tested the hypothesis of local adaptation in the aphid brevicoryne brassicae. aphid genotypes derived from two sympatric host plants, brassica oleracea and b. campestris, were assessed in order to measure the extent of phenotypic plasticity in morphological and life history tr ... | 2013 | 24265743 |
| free radical scavenging, antiproliferative activities and profiling of variations in the level of phytochemicals in different parts of broccoli (brassica oleracea italica). | in the present study, the seeds of broccoli (cultivar palam samridhi) at different stages of development were being analysed for their antioxidant and antiproliferative properties. among the antioxidant assays performed, a remarkable inhibition of superoxide radicals i.e. 94.25% observed with extracts of five days old sprout (ps5) at 2 mg/ml concentration. although, all the extracts showed high cytotoxicity but the floret extract (psf) found to be most effective with ic₅₀ value of 25.94 μg/ml wh ... | 2014 | 24262571 |
| field study on the accumulation of trace elements by vegetables produced in the vicinity of abandoned pyrite mines. | to evaluate the accumulation of trace elements (te) by vegetables produced in the vicinity of abandoned pyrite mines, eighteen different small farms were selected near three mines from the portuguese sector of the iberian pyrite belt (são domingos, aljustrel and lousal). total and bioavailable as, cu, pb, and zn concentrations were analyzed in the soils, and the same te were analyzed in three different vegetables, lettuce (lactuca sativa), coriander (coriandrum sativum), and cabbage (brassica ol ... | 2014 | 24252198 |
| high-throughput sequencing identification of novel and conserved mirnas in the brassica oleracea leaves. | plant micrornas are short (~21 nt) non-coding molecules that regulate gene expression by targeting the mrna cleavage or protein translation inhibition. in this manner, they play many important roles in the cells of living organisms. one of the plant species in which the entire set of mirnas has not been yet completely identified is brassica oleracea var. capitata (cabbage). for this reason and for the economic and nutritional importance of this food crop, high-throughput small rnas sequencing ha ... | 2013 | 24245539 |
| host chemical footprints induce host sex discrimination ability in egg parasitoids. | trissolcus egg parasitoids, when perceiving the chemical footprints left on a substrate by pentatomid host bugs, adopt a motivated searching behaviour characterized by longer searching time on patches were signals are present. once in contact with host chemical footprints, trissolcus wasps search longer on traces left by associated hosts rather than non-associated species, and, in the former case, they search longer on traces left by females than males. based on these evidences, we hypothesized ... | 2013 | 24244417 |
| brpspl9 (brassica rapa ssp. pekinensis spl9) controls the earliness of heading time in chinese cabbage. | the leafy heads of cabbage (brassica oleracea), chinese cabbage (brassica rapa ssp. pekinensis), brussels sprouts (b. oleracea ssp. gemmifera) and lettuce (lactuca sativa) comprise extremely incurved leaves that are edible vegetable products. the heading time is important for high quality and yield of these crops. here, we report that brpspl9-2 (b. rapa ssp. pekinensis squamosa promoter binding-like 9-2), a target gene of microrna brp-mir156, controls the heading time of chinese cabbage. quantit ... | 2014 | 24237584 |
| qtl mapping of leafy heads by genome resequencing in the ril population of brassica rapa. | leaf heads of cabbage (brassica oleracea), chinese cabbage (b. rapa), and lettuce (lactuca sativa) are important vegetables that supply mineral nutrients, crude fiber and vitamins in the human diet. head size, head shape, head weight, and heading time contribute to yield and quality. in an attempt to investigate genetic basis of leafy head in chinese cabbage (b. rapa), we took advantage of recent technical advances of genome resequencing to perform quantitative trait locus (qtl) mapping using 15 ... | 2013 | 24204591 |
| the broccoli (brassica oleracea) phloem tissue proteome. | the transport of sugars, hormones, amino acids, proteins, sugar alcohols, and other organic compounds from the sites of synthesis to the sites of use or storage occurs through the conducting cells of the phloem. to better understand these processes a comprehensive understanding of the proteins involved is required. while a considerable amount of data has been obtained from proteomic analyses of phloem sap, this has mainly served to identify the soluble proteins that are translocated through the ... | 2013 | 24195484 |
| intra-specific variation in wild brassica oleracea for aphid-induced plant responses and consequences for caterpillar-parasitoid interactions. | herbivore-induced plant responses not only influence the initiating attackers, but also other herbivores feeding on the same host plant simultaneously or at a different time. insects belonging to different feeding guilds are known to induce different responses in the host plant. changes in a plant's phenotype not only affect its interactions with herbivores but also with organisms higher in the food chain. previous work has shown that feeding by a phloem-feeding aphid on a cabbage cultivar facil ... | 2014 | 24178834 |
| transposon variation by order during allopolyploidisation between brassica oleracea and brassica rapa. | although many studies have shown that transposable element (te) activation is induced by hybridisation and polyploidisation in plants, much less is known on how different types of te respond to hybridisation, and the impact of te-associated sequences on gene function. we investigated the frequency and regularity of putative transposon activation for different types of te, and determined the impact of te-associated sequence variation on the genome during allopolyploidisation. we designed differen ... | 2014 | 24176077 |
| [effects of different nitrogen forms and their ratios on broccoli yield, quality, and nutrient absorption]. | a field experiment was conducted to study the effects of different nitrogen forms ((nh2)2co-n, no(3-)-n, nh(4+)-n, and no(3-)-nh4+) and their ratios on the yield formation, quality, and nutrient (n, p, and k) absorption of broccoli (brassica oleracea). fertilization with no(3-)-n increased the accumulation of nitrate and soluble sugars in ball flower. when the no(3-)-n: nh(4+)-n ratio was ranged from 3:7 to 5:5 and from 5:5 to 7:3, respectively, the accumulation of nitrate in the ball flower was ... | 2013 | 24175523 |
| genome-wide identification and characterization of mirnas in the hypocotyl and cotyledon of cauliflower (brassica oleracea l. var. botrytis) seedlings. | micrornas (mirnas) are a class of small endogenous, non-coding rnas that have key regulatory functions in plant growth, development, and other biological processes. hypocotyl and cotyledon are the two major tissues of cauliflower (brassica oleracea l. var. botrytis) seedlings. tissue culture experiments have indicated that the regenerative abilities of these two tissues are significantly different. however, the characterization of mirnas and their roles in regulating organ development in caulifl ... | 2014 | 24170336 |
| influence of day length and temperature on the content of health-related compounds in broccoli (brassica oleracea l. var. italica). | vegetables grown at different latitudes are exposed to various temperatures and day lengths, which can affect the content of health- and sensory-related compounds in broccoli florets. a 2 × 2 factorial experiment was conducted under controlled growth conditions, with contrasting temperatures (15/9 and 21/15 °c) and day lengths (12 and 24 h), to investigate the effect on glucosinolates, vitamin c, flavonols, and soluble sugars. aliphatic glucosinolates, quercetin, and kaempferol were at their hig ... | 2013 | 24168154 |
| metabolic differentiation of diamondback moth ( plutella xylostella (l.)) resistance in cabbage ( brassica oleracea l. ssp. capitata). | the diamondback moth, plutella xylostella (l.), is a major pest responsible for destroying cabbage and other brassica vegetable crops. a diamondback moth-resistant cabbage line was studied by comparing its metabolite profiles with those of a susceptible cabbage. fourier transform infrared spectroscopy analysis revealed that carbohydrates, aromatic compounds, and amides were the major factors that distinguished the resistant and susceptible genotypes. gas chromatography-time-of-flight mass spectr ... | 2013 | 24144435 |
| variations in the phytochemical contents and antioxidant capacity of organically and conventionally grown italian cauliflower (brassica oleracea l. subsp. botrytis): results from a three-year field study. | a three-year field study (2009-2011) was performed to evaluate phytochemicals and antioxidant capacities of two genotypes (hf1 emeraude and the local variety, velox) of green cauliflower grown under organic and conventional management. the conventional system increased yield, but had little effect on the dry matter, whereas the organic system increased the soluble solids. phytochemicals and antioxidant capacity showed significant year-to-year variability. during the third year, the scarce rainfa ... | 2013 | 24134670 |
| quantification of glucosinolates, anthocyanins, free amino acids, and vitamin c in inbred lines of cabbage (brassica oleracea l.). | we profiled and quantified glucosinolates (gsls), anthocyanins, free amino acids, and vitamin c metabolites in forty-five lines of green and red cabbages. analysis of these distinct cabbages revealed the presence of 11 gsls, 13 anthocyanins, 22 free amino acids, and vitamin c. gsl contents were varied amongst the different lines of cabbage. the total gsl content was mean 10.6 μmol/g dw, and sinigrin was the predominant gsl accounted mean 4.0 μmol/g dw (37.7% of the total) followed by glucoraphan ... | 2014 | 24128451 |
| spent mushroom waste as a media replacement for peat moss in kai-lan (brassica oleracea var. alboglabra) production. | peat moss (pm) is the most widely used growing substrate for the pot culture. due to diminishing availability and increasing price of pm, researchers are looking for viable alternatives for peat as a growth media component for potted plants. a pot study was conducted with a view to investigate the possibility of using spent mushroom waste (smw) for kai-lan (brassica oleracea var. alboglabra) production replacing peat moss (pm) in growth media. the treatments evaluated were 100% pm (control), 100 ... | 2013 | 24106452 |
| age-related macular degeneration: effects of a short-term intervention with an oleaginous kale extract--a pilot study. | age-related macular degeneration (amd) is a multifactorial degenerative disease of the retina, which accounts for slowly progressive visual impairment in the elderly. an increased dietary intake of xanthophylls is suggested to be inversely related to the risk of macular disease. | 2014 | 24103519 |
| nutritional quality changes throughout shelf-life of fresh-cut kailan-hybrid and 'parthenon' broccoli as affected by temperature and atmosphere composition. | the nutritional quality changes of the fresh-cut kailan-hybrid broccoli were compared with those of the 'parthenon' cv. throughout 15 days at 2 ℃, 5 ℃ and 8 ℃ under air and modified atmosphere packaging. florets showed higher dietary fiber content than stems. the total protein content of kailan-hybrid florets was 2.2-fold higher than that of 'parthenon' cv. and higher amounts of s, ca, mg, fe, sr, mn, zn and cu were found. however, 'parthenon' florets registered higher initial total phenolics co ... | 2015 | 24045885 |
| effect of brassica oleracea in rats skin wound healing. | to investigate the effect of brassica oleracea herbal balsam on the healing of skin wounds in rats. | 2013 | 24000060 |
| supercritical co2 extraction, chemical characterisation and antioxidant potential of brassica oleracea var capitata against ho·, o2(·-) and roo·. | in this work were extracted bioactive compounds from brassica oleracea var capitata using supercritical co2 and evaluated the antioxidant potential of the extracts. five extractions were accomplished to investigate the influence of pressure (10-25 mpa) and temperature (20-60 °c) in the extraction yield, chemical composition and antioxidant potential towards peroxyl, superoxide and hydroxyl radicals. the highest extraction yield was obtained at 60 °c and 25 mpa, which was 0.47 wt% (run 2). in the ... | 2013 | 23993571 |
| comparative study of the effects of laser photobiomodulation and extract of brassica oleracea on skin wounds in wistar rats: a histomorphometric study. | the objective of this study was to investigate the effect of a photobiomodulation laser and brassica oleracea on tissue morphology in skin wounds. the parameters analyzed were type i and iii collagen fibers, and thickness and surface density of the epithelial tissue, as well as how quickly the wound closed. five skin wounds 12mm in diameter were made on the backs of the animals, which were randomized into four groups (8 animals each). saline group: 0.9% saline solution; ointment group (extract o ... | 2013 | 23968696 |
| cabbage waxes affect trissolcus brochymenae response to short-range synomones. | we show that induced synomones, emitted as a consequence of murgantia histrionica activity on brassica oleracea, are adsorbed by the epicuticular waxes of leaves and perceived by the egg parasitoid trissolcus brochymenae. leaves were exposed to m. histrionica females placed on the abaxial leaf surface. after 24 h, the leaves were treated mechanically using gum arabic, or chemically using chloroform, on the adaxial surface, and finally the adaxial surface was assayed with t. brochymenae by two-ch ... | 2013 | 23956054 |
| uptake and accumulation of four ppcp/edcs in two leafy vegetables. | many pharmaceutical and personal care products (ppcps) and endocrine-disrupting chemicals (edcs) are present in reclaimed water, leading to concerns of human health risks from the consumption of food crops irrigated with reclaimed water. this study evaluated the potential for plant uptake and accumulation of four commonly occurring ppcp/edcs, i.e., bisphenol a (bpa), diclofenac sodium (dcl), naproxen (npx), and 4-nonylphenol (np), by lettuce (lactuca sativa) and collards (brassica oleracea) in h ... | 2013 | 23911624 |
| extraction, chemical characterization and biological activity determination of broccoli health promoting compounds. | broccoli (brassica oleracea l. var. italica) contains substantial amount of health-promoting compounds such as vitamins, glucosinolates, phenolic compounds, and dietary essential minerals; thus, it benefits health beyond providing just basic nutrition, and consumption of broccoli has been increasing over the years. this review gives an overview on the extraction and separation techniques, as well as the biological activity of some of the above mentioned compounds which have been published in the ... | 2013 | 23899380 |
| or mutation leads to photo-oxidative stress responses in cauliflower (brassica oleracea) seedlings during de-etiolation. | the orange (or) gene is a gene mutation that can increase carotenoid content in plant tissues normally devoid of pigments. it affects plastid division and is involved in the differentiation of proplastids or non-colored plastids into chromoplasts. in this study, the de-etiolation process of the wild type (wt) cauliflower (brassica oleracea l. var. botrytis) and or mutant seedlings was investigated. we analyzed pigment content, plastid development, transcript abundance and protein levels of genes ... | 2013 | 23887833 |
| site-specific gene targeting using transcription activator-like effector (tale)-based nuclease in brassica oleracea. | site-specific recognition modules with dna nuclease have tremendous potential as molecular tools for genome targeting. the type iii transcription activator-like effectors (tales) contain a dna binding domain consisting of tandem repeats that can be engineered to bind user-defined specific dna sequences. we demonstrated that customized tale-based nucleases (talens), constructed using a method called "unit assembly", specifically target the endogenous frigida gene in brassica oleracea l. var. capi ... | 2013 | 23870552 |
| shifts in the evolutionary rate and intensity of purifying selection between two brassica genomes revealed by analyses of orthologous transposons and relics of a whole genome triplication. | recent sequencing of the brassica rapa and brassica oleracea genomes revealed extremely contrasting genomic features such as the abundance and distribution of transposable elements between the two genomes. however, whether and how these structural differentiations may have influenced the evolutionary rates of the two genomes since their split from a common ancestor are unknown. here, we investigated and compared the rates of nucleotide substitution between two long terminal repeats (ltrs) of ind ... | 2013 | 23869625 |
| intraspecific variation of host plant and locality influence the lepidopteran-parasitoid system of brassica oleracea crops. | the aim of the study was to investigate the attractiveness to herbivores and parasitoids of two cultivars of brassica oleracea l., namely, b. oleracea variety acephala (kale) and b. oleracea variety capitata (cabbage), that exhibit differences of morphological and biochemical traits. to this end, field samplings were replicated at seven localities in galicia (northwestern spain). three specialist and three generalist lepidopteran species were sampled. in total, 7,050 parasitoids were obtained, b ... | 2013 | 23865177 |
| modeling the integration of parasitoid, insecticide, and transgenic insecticidal crop for the long-term control of an insect pest. | the tools of insect pest management include host plant resistance, biological control, and insecticides and how they are integrated will influence the durability of each. we created a detailed model of the population dynamics and population genetics of the diamondback moth, plutella xylostella l., and its parasitoid, diadegma insulare (cresson), to study long-term pest management in broccoli brassica oleracea l. given this pest's history of evolving resistance to various toxins, we also evaluate ... | 2013 | 23865173 |
| involvement of a glucosinolate (sinigrin) in the regulation of water transport in brassica oleracea grown under salt stress. | members of the brassicaceae are known for their contents of nutrients and health-promoting phytochemicals, including glucosinolates. the concentrations of these chemopreventive compounds (glucosinolate-degradation products, the bioactive isothiocyanates) may be modified under salinity. in this work, the effect of the aliphatic glucosinolate sinigrin (2-propenyl-glucosinolate) on plant water balance, involving aquaporins, was explored under salt stress. for this purpose, water uptake and its tran ... | 2014 | 23837634 |
| postharvest circadian entrainment enhances crop pest resistance and phytochemical cycling. | the modular design of plants enables individual plant organs to manifest autonomous functions and continue aspects of metabolism, such as respiration, even after separation from the parent plant. therefore, we hypothesized that harvested vegetables and fruits may retain capacity to perceive and respond to external stimuli. for example, the fitness advantage of plant circadian clock function is recognized; however, whether the clock continues to influence postharvest physiology is unclear. here w ... | 2013 | 23791724 |
| metabolite profiling approach reveals the interface of primary and secondary metabolism in colored cauliflowers (brassica oleracea l. ssp. botrytis). | in the present study, carotenoids, anthocyanins, and phenolic acids of cauliflowers ( brassica oleracea l. ssp. botrytis) with various colored florets (white, yellow, green, and purple) were characterized to determine their phytochemical diversity. additionally, 48 metabolites comprising amino acids, organic acids, sugars, and sugar alcohols were identified using gas chromatography-time-of-flight mass spectrometry (gc-tofms). carotenoid content was considerably higher in green cauliflower; antho ... | 2013 | 23782237 |
| plants know where it hurts: root and shoot jasmonic acid induction elicit differential responses in brassica oleracea. | plants respond to herbivore attack by rapidly inducing defenses that are mainly regulated by jasmonic acid (ja). due to the systemic nature of induced defenses, attack by root herbivores can also result in a shoot response and vice versa, causing interactions between above- and belowground herbivores. however, little is known about the molecular mechanisms underlying these interactions. we investigated whether plants respond differently when roots or shoots are induced. we mimicked herbivore att ... | 2013 | 23776489 |
| comparison of bioactive phytochemical content and release of isothiocyanates in selected brassica sprouts. | the consumption of brassica sprouts as raw vegetables provides a fair amount of glucosinolates (gls) and active plant myrosinase, which enables the breakdown of gls into health-promoting isothiocyanates (itcs). this study reports the determination of the main constituents related to human health found in edible sprouts of two brassica oleracea varieties, broccoli and tuscan black kale, and two raphanus sativus varieties, daikon and sango. radish sprouts exhibited the highest ability to produce i ... | 2013 | 23768361 |
| assessment of the anticancer compounds se-methylselenocysteine and glucosinolates in se-biofortified broccoli (brassica oleracea l. var. italica) sprouts and florets. | broccoli (brassica oleracea l. var. italica) is a rich source of chemopreventive compounds. here, we evaluated and compared the effect of selenium (se) treatment on the accumulation of anticancer compounds se-methylselenocysteine (semscys) and glucosinolates in broccoli sprouts and florets. total se and semscys content in sprouts increased concomitantly with increasing se doses. selenate was superior to selenite in inducing total se accumulation, but selenite is equally effective as selenate in ... | 2013 | 23763668 |
| potential of cultivar and crop management to affect phytochemical content in winter-grown sprouting broccoli (brassica oleracea l. var. italica). | variety and crop management strategies affect the content of bioactive compounds (phenolics, flavonoids and glucosinolates) in green broccoli (calabrese) types, which are cultivated during summer and autumn in temperate european climates. sprouting broccoli types are morphologically distinct and are grown over the winter season and harvested until early spring. thus they show considerable potential for development as an import substitution crop for growers and consumers during the 'hungry gap' o ... | 2014 | 23761132 |
| comparison of leafy kale populations from italy, portugal, and turkey for their bioactive compound content: phenolics, glucosinolates, carotenoids, and chlorophylls. | kales are primitive leafy brassica oleracea l. forms, widespread in local farming systems of several european countries and employed in the preparation of traditional recipes. kales are also potential sources of healthy bioactive phytochemical components. the present study compared the bioactive compound content of kale populations from italy, portugal, and turkey, either from local sources or grown in an experimental field. | 2013 | 23749678 |
| quantitative trait loci analysis of non-enzymatic glucosinolate degradation rates in brassica oleracea during food processing. | epidemiological and mechanistic studies show health-promoting effects of glucosinolates and their breakdown products. in literature, differences in non-enzymatic glucosinolate degradation rates during food processing between different vegetables are described, which provide the basis for studying the genetic effects of this trait and breeding vegetables with high glucosinolate retention during food processing. non-enzymatic glucosinolate degradation, induced by heat, was studied in a publicly av ... | 2013 | 23748744 |
| use of heat stress responsive gene expression levels for early selection of heat tolerant cabbage (brassica oleracea l.). | cabbage is a relatively robust vegetable at low temperatures. however, at high temperatures, cabbage has disadvantages, such as reduced disease tolerance and lower yields. thus, selection of heat-tolerant cabbage is an important goal in cabbage breeding. easier or faster selection of superior varieties of cabbage, which are tolerant to heat and disease and have improved taste and quality, can be achieved with molecular and biological methods. we compared heat-responsive gene expression between a ... | 2013 | 23736694 |
| role of mannitol metabolism in the pathogenicity of the necrotrophic fungus alternaria brassicicola. | in this study, the physiological functions of fungal mannitol metabolism in the pathogenicity and protection against environmental stresses were investigated in the necrotrophic fungus alternaria brassicicola. mannitol metabolism was examined during infection of brassica oleracea leaves by sequential hplc quantification of the major soluble carbohydrates and expression analysis of genes encoding two proteins of mannitol metabolism, i.e., a mannitol dehydrogenase (abmdh), and a mannitol-1-phospha ... | 2013 | 23717316 |
| testing insecticidal activity of novel chemically synthesized sirna against plutella xylostella under laboratory and field conditions. | over the last 60 years, synthetic chemical pesticides have served as a main tactic in the field of crop protection, but their availability is now declining as a result of the development of insect resistance. therefore, alternative pest management agents are needed. however, the demonstration of rnai gene silencing in insects and its successful usage in disrupting the expression of vital genes opened a door to the development of a variety of novel, environmentally sound approaches for insect pes ... | 2013 | 23667556 |
| nonadverse effects on allergenicity of isopentenyltransferase-transformed broccoli. | genetically modified organisms (gmos) provide modern agriculture with improvements in efficiency and the benefits of enhanced food production; however, the potential impact of gmos on human health has not yet been clarified. | 2013 | 23654078 |
| deciphering the diploid ancestral genome of the mesohexaploid brassica rapa. | the genus brassica includes several important agricultural and horticultural crops. their current genome structures were shaped by whole-genome triplication followed by extensive diploidization. the availability of several crucifer genome sequences, especially that of chinese cabbage (brassica rapa), enables study of the evolution of the mesohexaploid brassica genomes from their diploid progenitors. we reconstructed three ancestral subgenomes of b. rapa (n = 10) by comparing its whole-genome seq ... | 2013 | 23653472 |
| changes in semsc, glucosinolates and sulforaphane levels, and in proteome profile in broccoli (brassica oleracea var. italica) fertilized with sodium selenate. | the aim of this work was to analyze the effect of sodium selenate fortification on the content of selenomethyl selenocysteine (semsc), total glucosinolates and sulforaphane, as well as the changes in protein profile of the inflorescences of broccoli (brassica oleracea var. italica). two experimental groups were considered: plants treated with 100 μmol/l sodium selenate (final concentration in the pot) and control plants treated with water. fortification began 2 weeks after transplantation and wa ... | 2013 | 23652991 |
| low and moderate photosynthetically active radiation affects the flavonol glycosides and hydroxycinnamic acid derivatives in kale (brassica oleracea var. sabellica) dependent on two low temperatures. | kale (brassica oleracea var. sabellica) contains a large number of naturally occurring structurally different non-acylated and acylated flavonol glycosides as well as hydroxycinnamic acid derivatives. the objective of this study was to determine the effect of low and moderate photosynthetic active radiation (par) and how these levels interact with low temperature in these phenolic compounds. juvenile kale plants were treated with par levels from 200 to 800 μmol m(-2) s(-1) at 5 and 10 °c under d ... | 2013 | 23647885 |
| protective role of (rs )-glucoraphanin bioactivated with myrosinase in an experimental model of multiple sclerosis. | the discovery of new natural compounds with pharmacological properties is a field of interest widely growing. recent literature shows that brassica vegetables (cruciferae) possess therapeutic effects particularly ascribed due to their content in glucosinolates, which upon myrosinase hydrolysis release the corresponding isothiocyanates. this study examines the potential neuroprotective and immunomodulatory effects of (rs )-glucoraphanin from tuscan black kale (brassica oleracea l. var. acephala s ... | 2013 | 23638842 |
| fungal-specific transcription factor abpf2 activates pathogenicity in alternaria brassicicola. | alternaria brassicicola is a successful saprophyte and necrotrophic plant pathogen. to identify molecular determinants of pathogenicity, we created non-pathogenic mutants of a transcription factor-encoding gene, abpf2. the frequency and timing of germination and appressorium formation on host plants were similar between the non-pathogenic ∆abpf2 mutants and wild-type a. brassicicola. the mutants were also similar in vitro to wild-type a. brassicicola in terms of vegetative growth, conidium produ ... | 2013 | 23617599 |
| identification of sequence-related amplified polymorphism markers linked to the red leaf trait in ornamental kale (brassica oleracea l. var. acephala). | artistic diversiform leaf color is an important agronomic trait that affects the market value of ornamental kale. in the present study, genetic analysis showed that a single-dominant gene, re (red leaf), determines the red leaf trait in ornamental kale. an f2 population consisting of 500 individuals from the cross of a red leaf double-haploid line 'd05' with a white leaf double-haploid line 'd10' was analyzed for the red leaf trait. by combining bulked segregant analysis and sequence-related amp ... | 2013 | 23613233 |
| acclimation to elevated co2 increases constitutive glucosinolate levels of brassica plants and affects the performance of specialized herbivores from contrasting feeding guilds. | plants growing under elevated co2 concentration may acclimate by modifying chemical traits. most studies have focused on the effects of environmental change on plant growth and productivity. potential effects on chemical traits involved in resistance, and the consequences of such effects on plant-insect interactions, have been largely neglected. here, we evaluated the performance of two brassica specialist herbivores from contrasting feeding guilds, the leaf-feeding pieris brassicae and the phlo ... | 2013 | 23609163 |
| enhanced resistance to spodoptera litura in endophyte infected cauliflower plants. | endophytic fungi, which live within host plant tissues without causing any visible symptom of disease, are important mediators of plant-herbivore interactions. these endophytes enhance resistance of host plant against insect herbivores mainly by productions of various alkaloid based defensive compounds in the plant tissue or through alterations of plant nutritional quality. two endophytic fungi, i.e., nigrospora sp. and cladosporium sp., were isolated from tinospora cordifolia (thunb.) miers, a ... | 2013 | 23575013 |
| genetic regulation of glucoraphanin accumulation in beneforté broccoli. | · diets rich in broccoli (brassica oleracea var italica) have been associated with maintenance of cardiovascular health and reduction in risk of cancer. these health benefits have been attributed to glucoraphanin that specifically accumulates in broccoli. the development of broccoli with enhanced concentrations of glucoraphanin may deliver greater health benefits. · three high-glucoraphanin f1 broccoli hybrids were developed in independent programmes through genome introgression from the wild sp ... | 2013 | 23560984 |
| homoeologous gsl-elong gene replacement for manipulation of aliphatic glucosinolates in brassica rapa l. by marker assisted selection. | aliphatic glucosinolates are the predominant sulfur-rich plant secondary metabolites in economically important brassica crops. glucosinolates and their hydrolysis products are involved in plant-microbe, plant-insect, plant-animal, and plant-human interactions. it is, therefore, important to manipulate glucosinolate profiles and contents in brassica species. in this study, aliphatic glucosinolates were genetically manipulated through homoeologous recombination in backcross lines followed by marke ... | 2013 | 23532458 |