interaction of phospholipid vesicles with rat hepatocytes in primary monolayer culture.we studied the interaction of positively and negatively charged unilamellar and multilamellar phospholipid vesicles (liposomes) with rat-liver parenchymal cells in primary monolayer culture. radioactive liposomal phosphatidylcholine was taken up more rapidly and to a larger extent from unilamellar than from multilamellar vesicles. no significant difference in uptake characteristics was observed between vesicles of different charge. the presence of serum greatly reduced uptake of liposomal phosph ...1978687666
vascular permeability alterations to horseradish peroxidase in experimental brain injury.protein uptake and transport within the brain stem vasculature of mechanically brain injured cats was studied by means of both light and electron microscopy utilizing intravenously injected horseradish peroxidase as the protein tracer. in animals sustaining low grade head injuries not of sufficient intensity to elicit either microscopic, intraparenchymal hemorrhages or subtle, neuropathological responses, peroxidase extravasation was noted both in the vascular walls and in the surrounding parenc ...1978687980
dopamine and noradrenaline neurons projecting to the septal area in the rat.the organisation of the catecholamine innervation of the rat spetal area was investigated by means of the glyoxylic acid fluorescence method in combination with dopamine uptake studies, lesions and retrograde tracing of horseradish peroxidase. the following catecholamine systems to the septum could be established: 1. the locus coreuleus noradrenergic system. these axons are widespread in the septum forming a moderately dense innervation in the anterior hippocampus, the medial septal nucleus, the ...1978688339
amygdaloid projections to the mesencephalon, pons and medulla oblongata in the cat.amygdalotegmental projections were studied in 26 cats after injections of horseradish peroxidase (hrp) in the diencephalon, midbrain and lower brain stem and in 6 cats after injection of 3h-leucine in the amygdala. following hrp injections in the posterior hypothalamus, periaqueductal gray (pag) and tegmentum many retrogradely labeled neurons were present in the central nucleus (ce) of the amygdala, primarily ipsilaterally. injections of hrp in the posterior hypothalamus and mesencephalon also r ...1978689127
the afferent connections of the main and the accessory olfactory bulb formations in the rat: an experimental hrp-study.the afferent connections of the main and accessory olfactory bulbs in the rat were examined by injecting horseradish peroxidase (hrp) into one or the other of these structures either by microelectrophoresis or by hydraulic pressure. alternate sections were stained with newly developed hrp-procedures using either benzidine dihydrochloride (de olmos and heimer, '77) or tetramethyl-benzidine. eighteen to twenty-four hours after unilateral hrp injections confined to the main olfactory bulb, a large ...1978690266
nuclei of the solitary tract: efferent projections to the lower brain stem and spinal cord of the cat.the efferent projections from the solitary complex to the lower brain stem and spinal cord were studied in the cat with the autoradiographic anterograde axonal transport and retrograde horseradish peroxidase (hrp) techniques. a revised cytoarchitectonic description of the caudal two-thirds of the complex is presented in which the complex was subdivided into six nuclei: lateral, ventrolateral, intermediate, medial, parvocellular, and commissural solitary tract nuclei. following injections of 3h a ...1978690272
distribution of motoneurones to the neck muscles, biventer cervicis, splenius and complexus in the cat.the distribution of motoneurones to the cat dorsal neck muscles biventer cervicis, splenius and complexus has been examined using the technique of retrograde horseradish peroxidase transport. a comparison was initially made of hrp uptake into motoneurones produced either by injecting hrp solution directly into a single neck muscle or by exposing the cut ends of motor nerves to an hrp solution. the injection of hrp into single neck muscles was found to produce widespread labelling of motoneurones ...1978690273
spinal and medullary input to the lateral cervical nucleus.the distributions of spinal and medullary cells projecting to the lateral cervical nucleus (lcn) have been investigated in young cats and dogs using the retrograde horseradish peroxidase (hrp) technique. labeled spinal cells, whose axons contribute to the spinocervical tract (sct), were found at all levels of the spinal cord ipsilateral to the injection sites. no significant differences were found between cat and dog, nor between cases with single injections at different levels of the lcn. sct c ...1978690282
the localization of the motor neurons innervating the extraocular muscles in the oculomotor nuclei of the cat and rabbit, using horseradish peroxidase.the localization of the motor neurons innervating the extraocular muscles in the oculomotor nuclei of adult cats and rabbits was investigated by means of retrograde labelling with horseradish peroxidase (hrp). the groups consisting of the motor neurons innervating an individual muscle lay in the nucleus as elongated columns extending in a longitudinal direction. the position of each group in the transverse section varied according to the rostro-caudal level of the nucleus. in the cat and rabbit, ...1978690283
organization and projections of the neurons in the dorsal tegmental area of the rat.the organization and projection of the neurons in the dorsal tegmental area at the locus coeruleus level of the rat have been investigated by means of the horseradish peroxidase (hrp) method, combined with monoamine oxidase staining to identify the noradrenaline (na) neurons. judging from the anatomical aspects elucidated in this study, this area is composed of various different cell groups. although the functional role of these neuronal groups remains to be determined, the present study strongl ...1978690413
cells of origin of subcortical afferents to the caudate nucleus: a horseradish peroxidase study in the cat.the horseradish peroxidase tracing method was used to investigate the sources of subcortical input to the caudate nucleus in the cat. of considerable interest was the finding, not previously described, that labeled neurons were present outside of the intralaminar complex, in the ventral anterior and mediodorsal thalamic nuclei. another new finding was that retrogradely labeled cells were present within the basolateral amygdaloid nucleus, following the caudate injections. in addition, neurons con ...1978698789
trigeminal ascending projections in the rabbit, studied with horseradish peroxidase. 1978698827
the t-tubule system in the myocardia of the sand rat and mouse as demonstrated by horseradish peroxidase.the transverse tubule (t-tubule) system in papillary muscles of the sand rat and the mouse were studied with the aid of a diffusion tracer (horseradish peroxidase). the t-tubule system in the sand rat showed a typical mammalian pattern with sarcolemmal tubules invaginating at the z-band level of the sarcomere. these tubules follow a transverse direction in the cell with frequent longitudinal side-branches which connect tubules at different z-band levels. in the mouse myocardium, the t-tubules al ...1978699013
studies on the ultrastructure and permeability of the hemotrichorial placenta. i. intercellular junctions of layer i and tracer administration into the maternal compartment.the distribution of lanthanum chloride and horseradish peroxidase within the full-term chorioallantoic placenta of the rat was investigated 1 to 20 min after administration of these tracers into the maternal blood circulation. both tracers rapidly penetrate trophoblastic layer i and diffuse into the interspace between layers i and ii. they are localized in extensive infoldings and caveolae of the outer surface of layer ii. the syncytial character of layer ii is confirmed at this development stag ...1978699024
studies on the ultrastructure and permeability of the hemotrichorial placenta. ii. fetal capillaries and tracer administration into the fetal blood circulation.the distribution of horseradish peroxidase and lanthanum chloride within the full term chorioallantoic placenta of the rat was examined after administration of these tracers into the umbilical artery. both tracers rapidly traverse the capillary endothelium. transendothelial channels, fenestrations and micropinocytotic vesicles provide the main pathways. intercellular clefts which are either patent or interrupted by leaky intercellular junctions, also contribute to a rapid passage of low and high ...1978699025
the t-tubule system in the specialized and general myocardium of the rat.the t-tubule system in cardiac muscle cells has been investigated with the electron microscope in 10 adult rats after infiltration with horseradish peroxidase. all cardiac muscle cells possess a t-system, but its complexity varies according to the region of the heart. it is most extensive in the general ventricular myocardium where there are primary, secondary and tertiary transverse tubules as well as longitudinal elements, and there are numerous couplings between the t-system and the sarcoplas ...1978701190
retrieval and recycling of synaptic vesicle membrane in pinched-off nerve terminals (synaptosomes).the morphological features of pinched-off presynaptic nerve terminals (synaptosomes) from rat brain were examined with electron microscope techniques; in many experiments, an extracellular marked (horseradish peroxidase or colloidal thorium dioxide) was included in the incubation media. when incubated in physiological saline, most terminals appeared approximately spherical, and were filled with small (approximately 400-a diameter) "synaptic vesicles"; mitochondria were also present in many of th ...1978701355
distribution of horseradish peroxidase (hrp)-anti-hrp immune complexes in mouse spleen with special reference to follicular dendritic cells.the distribution of immune complexes has been studied in mouse spleen stimulated to contain many germinal centers (gc's). horseradish peroxidase (hrp)-anti-hrp complexes were used as an appropriately precise and sensitive model. we were primarily interested in the relative abilities of three cell types to interact with complexes: lymphocytes, macrophages, and follicular dendritic cells (fdc's). the latter are distinctive, nonendocytic, stellate cells located primarily at the transition of mantle ...1978701370
open junctions in the endothelium of the postcapillary venules of the diaphragm.we have previously established that approximately 30% of the endothelial junctions in the pericytic venules of the mouse diaphragm are open to a gap of approximately 30--60 a, and are fully permeated by hemeundecapeptide (h11p) (mol diam approximately 20 a). to estimate the size limit for molecules that can permeate these junctions, we have administered graded tracers intravenously and studied their behavior at the level of pericytic venules in bipolar microvascular fields (bmfs) in the mouse di ...1978701375
cell bodies of origin of reticular projections from the superior colliculus in the cat: an experimental study with the use of horseradish peroxidase as a use of the retrograde axonal transport of horseradish peroxidase (hrp), the projection from the superior colliculus (sc) to the brain stem reticular formation (rf) was investigated in the cat. a 0.2-0.5 microliter of a 50% suspension of sigma vi hrp was injected stereotactically in various portions of the pontomedullary rf, and, as a control to the injection to the rf, in the inferior olive or in the spinal cord. labeled cells were found within and deep to the intermediate gray layer of the s ...1978701486
laminar origins of spinothalamic projections in the cat as determined by the retrograde transport of horseradish peroxidase.the method of retrograde axonal transport of horseradish peroxidase (hrp) was used to identify the locations of cells of origin of the spinothalamic tract in the cat. injections of from 0.2-3.0 microliter of 30% hrp were made unilaterally in various regions of the somatosensory thalamus. massive injections of the caudal thalamus in several cats showed the spinothalamic cells of origin to be located mainly in laminae i, vii and viii in the lumbar enlargement, and in laminae i, v and vii-viii in t ...1978701487
anatomical and physiological properties of ipsilaterally projecting spinothalamic neurons in the second cervical segment of the cat's spinal cord.anatomical and electrophysiological methods were used to investigate the projections and response properties of neurons in the second cervical (c2) spinal segment of the cat giving origin to a previously undescribed projection to the ipsilateral thalamus. the method of retrograde axonal transport of horseradish peroxidase (hrp) was used to identify neurons in c2 giving rise to thalamic projections. following large (3.0 microliter) thalamic hrp injections, a large number of labeled neurons was ob ...1978701488
differential projections of cat medullary raphe neurons demonstrated by retrograde labelling following spinal cord lesions.neurons of the medullary raphe nuclei in cats were retrogradely labelled following injection of horseradish peroxidase (hrp) into the l6 spinal cord segment. brainstems were cut in sagittal section to facilitate examination of the rostral-caudal extent of raphe neurons projecting to the spinal cord. large numbers of hrp-labelled neurons were found in nucleus raphe magnus, nucleus raphe pallidus, and nucleus raphe obscurus (as well as a few neurons in nucleus raphe pontis). dorsal or ventral hemi ...1978701490
the olivocerebellar projection in the cat studied with the method of retrograde axonal transport of horseradish peroxidase. vii. the projection to lobulus simplex, crus i and ii.the olivocerebellar projection to lobulus simplex, crus i and ii in the cat was investigated by means of retrograde axonal transport of horseradish peroxidase (hrp). the distribution of labeled cells in the inferior olive following hrp injections in lobulus simplex, crus i and ii confirmed the findings by brodal ('40b) that the rostral half of the principal olive projects to these areas of the cerebellar hemisphere. however, concerning details there are some differences in so far as the heaviest ...1978701495
a morphological study of the axons and recurrent axon collaterals of cat alpha-motoneurones supplying different hind-limb muscles.1. intracellular injections with horseradish peroxidasewere performed in cat alpha-motoneurones supplying various hind-limb muscles. 2. ten alpha-motoneurones from each of the quadriceps, posterior biceps, gastrocnemius-soleus and anterior tibial pools as well as from the pool supplying the short plantar muscles were collected for morphological analysis of the intramedullary axonal systems including the recurrent axon collaterals. 3. the diameter of the alpha-motor axons showed considerable vari ...1978702381
a morphological study of the axons and recurrent axon collaterals of cat alpha-motoneurones supplying different functional types of muscle unit.1. cat alpha-motoneurones supplying muscle units of the soleus s type and gastrocnemius s, fr and ff types were injected intracellularly with horseradish peroxidase. 2. ten alpha-motoneurones of each motor unit type were collected for morphological analysis of the intramedullary parts of their axonal systems including the recurrent axon collaterals. 3. the alpha-motor axon diameters in the spinal cord white matter were significantly smaller for the soleus motor units (mean = 5.2 micrometer) than ...1978702385
ultrastructural studies on blood-brain barrier dysfunction after cerebral air embolism in the rat.male albino rats were anaesthetized with diazepam, injected with horseradish peroxidase and evans blue-labeled albumin and given an embolus of 0.01 ml air in the right common carotid artery after ligation of the external carotid branch. the pial arteries of the right cerebral hemisphere were stained blue, particularly the middle cerebral artery and its main arterial branchlets. ultrastructurally, some endothelial cells in the right middle cerebral artery, small arteries and arterioles showed a d ...1978707046
sites of cerebrovascular injury induced by radiographic contrast media.the object of this study was to determine the type of cerebral vessel affected by injection of radiopaque contrast agents used in cerebral angiography. seventeen rabbits were prepared surgically for a left intracarotid injection of methylglucamine iothalamate (conray 60) or methylglucamine diatrizoate (reno-m-60). extravasations of the tracers, evans blue and horseradish peroxidase, occurred in the left half of the brain and occasionally in the right half. within those areas of blood-brain barri ...1978707323
developmental organization of raphe serotonin neuron groups in the rat.the pre- and early postnatal development of serotonin neurons in the rat brainstem was studied using the fluorescence histochemical method. the technique utilized does not require drug pretreatment to visualize an intense serotonin fluorophore localized in neuronal perikarya, dendrites, and axons. all the serotonin neuron groups develop as bilateral nuclei which extend from the midbrain through the medulla. six of the nine groups undergo a midline fusion from embryonic day 18 (e 18) through post ...1978707816
protein thiolation and reversible protein-protein conjugation. n-succinimidyl 3-(2-pyridyldithio)propionate, a new heterobifunctional reagent.a heterobifunctional reagent, n-succinimidyl 3-(2-pyridyldithio)propionate, was synthesized. its n-hydroxysuccinimide ester group reacts with amino groups and the 2-pyridyl disulphide structure reacts with aliphatic thiols. a new thiolation procedure for proteins is based on this reagent. the procedure involves two steps. first, 2-pyridyl disulphide structures are introduced into the protein by the reaction of some of its amino groups with the n-hydroxysuccinimide ester sie of the reagent. the p ...1978708370
a study of sensory projection from jaw muscles to the cerebral cortex in the rat.the sensory projection from jaw muscles to the cerebral cortex have been studied in rats by electrophysiological and histochemical methods. electrical stimulation of individual masticatory muscles elicited bilateral responses in the cortical areas 8, 10, 2, and 2a. the following pathway was postulated to mediate these cortical responses; impulses of muscle origin are conducted in turn to the trigeminal mesencephalic tract nucleus (tmt), the contralateral thalamic nucleus ventralis posteromediali ...1978713182
subcortical projections to the prefrontal cortex in the rat as revealed by the horseradish peroxidase technique. 1978714251
long ascending projections from substantia gelatinosa rolandi and the subjacent dorsal horn in the rat.small neurons of the substantia gelatinosa rolandi and the subjacent dorsal horn of the spinal cord have been thought to exert a direct modulatory effect only on neurons located within a distance of a few spinal segemnts. by using the technique of retorograde transport of horseradish peroxidase, however, it has been found that in the rat a significant number of these cells, particularly those of the subjacent dorsal horn, ascend many spinal segments to the lateral cervical nucleus and to the low ...1978715454
synapses of optic nerve afferents in the rat suprachiasmatic nucleus. i. identification, qualitative description, development and distribution.synapses of optic nerve afferents (optic synapses) in the rat suprachiasmatic nucleus (scn) have been identified ultrastructurally. they are easily distinguished from other types of synapses. the optic boutons are characterized by the presence of large mitochondria with a swollen electron lucent matrix and an interconnected tubular system formed by their inner membrane. other, more variable features include: 1) a scattered pattern of synaptic vesicles which are found throughout the entire presyn ...1978719729
surface functions during mitosis i: phagocytosis, pinocytosis and mobility of surface-bound con a.phagocytosis, pinocytosis and the surface distribution of concanavalin a (cona) have been analyzed during mitosis in several mammalian cell lines. use of the bisbenzimidazole dye, hoechst 33258, for chromosome staining after gentle fixation made possible the rapid identification and correlation of mitotic phase with surface properties. phagocytosis of both opsonized and nonopsonized particles is markedly depressed in mitotic cells of the mouse macrophage cell line j774.1. the uptake of opsonized ...1978719746
removal of horseradish peroxidase and fluorescein-labelled dextran from csf spaces of rabbit optic nerve. a light and electron microscope study. 1978720432
topographical organization of the striatonigral pathway revealed by anterograde and retrograde neuroanatomical tracing techniques.l-[4,5-3h]leucine was injected stereotaxically into various regions of the rat neostriatum. light microscopic autoradiographic techniques were used to plot the entire efferent pathways of the neostriatum, and in particular, the projections to the substantia nigra (sn). the terminal distribution of the pathways projecting to the ipsilateral sn was predominantly restricted to the zona reticulata region. the dorsal part of the head of the striatum was found to innervate the anterior and medial port ...1978721701
early atherosclerotic lesions in rat aorta. effect of cholesterol feeding and immunization.germ-free and conventional rats were fed cholesterol or normal pellets and part of both groups were immunized repeatedly with horseradish peroxidase (hrp). four days after the last injections all the rats were killed, aortas removed and prepared for immunocytology and chemical analysis. free peroxidase was not detected in the arterial wall and in all immunized rats only faint staining of free anti-hrp antibodies occurred in the intima. in contrast there was a consistent deposition of hrp-anti-hr ...1978725328
[capillary ultrastructure and permeability in the posterior lobe of the rat pituitary].the ultrastructure of capillaries and their permeability to horseradish peroxidase and ferritin in rat posterior pituitary has been studied. the capillary wall is formed mainly by flat endothelium penetrated by regularly situated fenestras. the majority of organelles is concentrated in the perikaryon. caveoles and pinocytotic vesicles are not numerous except for some perikaryons where they are concentrated along the basal surface. intracellular clefts are interrupted in the regions of fusion of ...1978726059
[comparative study of afferent connections in the habenula in rats and cats after injection of horseradish peroxidase].the habenular complex of 11 cats and 13 rats was injected with horse radish peroxydase (hrp) (30% solution, 0,05--0,4 microliter). retrograde labelling of cells was visualized after a survival period of 24 to 48 hours by means of the method of lavail et al. (1973). the resultats obtained with this method suggest significant species differences regarding the habenular afferents in cats and rats. one of the most striking differences is that the entopeduncular nucleus of the cat contains very few h ...1978728629
the effects of horseradish peroxidase on the concentration of 5-hydroxytryptamine and platelet counts in blood of rabbits and guinea pigs.when hrp was injected i.v. in rabbits and guinea pigs, a rapid fall in the number of circulating platelets and the concentration of 5 ht occurred. platelet aggregates were trapped in lung capillaries. the aggregation was reversible and the return of platelets to the circulation concomitant with a rise in blood 5 ht concentration.1978730554
the superior colliculus control of pinna movements in the cat: possible anatomical connections.possible anatomical pathways mediating superior colliculus control of pinna movements were determined in the cat using the orthograde autoradiographic tracing method and the retrograde horseradish peroxidase technique. this was done in the following manner. first, the division of the facial nucleus that innervates the pinna muscles was determined by injecting the pinna muscles with hrp and surveying the facial nucleus for retrogradely filled cells. second, the brainstem regions that project the ...1978730846
localization of sensory neurons traversing the stellate ganglion of the cat.the distribution of sensory cells whose axons traverse the stellate ganglion and project via sympathetic cardiac nerves to the heart of the cat has been examined quantitatively. horseradish peroxidase (hrp) injected at multiple sites in the right stellate ganglion, or applied to the middle cardiac nerve, labelled small numbers of cells in the thoracic dorsal root ganglia (drg) from t1 to t8. these cells were most numerous between t2 and t5 and were consistently small (less than 40 micrometer) re ...1978730853
features of foreign proteins affecting their retrograde transport in axons of the visual system.the retrograde axoplasmic transport of foreign proteins in the rat visual system shows certain specificities. the molecular features of these proteins which may underlie their entry into the retrograde phase have been examined using biochemical and morphologic techniques. of the isoenzymes of horseradish peroxidase (hrp), the basic isoenzyme c is strongly transported, while the acidic isoenzymes abeta and aalpha are transported weakly and not at all, respectively. decreasing the isoelectric poin ...1978731278
functional and anatomical consequences of neonatal visual cortical damage in superior colliculus of the golden hamster.1. in normal hamsters the visual cortex sends a retinotopically organized projection to the ipsilateral superior colliculus. 2. acute or chronic unilateral ablations of visual cortex in adult animals decrease the incidence of directionally selective cells encountered in the superficial laminae of the ipsilateral colliculus, but not in the deeper layers (those ventral to the stratum opticum). 3. unilateral ablations of visual cortex in infant hamsters induce an aberrant crossed projection to the ...1978731286
the distribution of motoneurones supplying chick hind limb muscles.1. the motor nuclei supplying many of the hind limb muscles were localized in late chick embryos (stage 36-37; 10-11 days) by utilizing the technique of retrograde transport of horseradish peroxidase. 2. each nucleus was found to be localized in a characteristic position in both the rostro-caudal and transverse plane of the spinal cord with only slight individual variation. 3. each motor nucleus consisted of an elongate, coherent cluster of labelled cells, with few cells occurring outside the cl ...1978731549
[pathways of substance penetration into the neurosecretory axons in the posterior lobe of the rat hypophysis].the penetration of horseradish peroxidase and ferritin into the neurosecretory axones in the rat's posterior pituitary has been studied with the electron microscope. markers were revealed in neurosecretory granules, in empty vesicles of the same size, in neurotubules, in lamellar bodies and in the axoplasm. markers were taken up from the extracellular space by micropinocytotic "synaptic" vesicles and probably by larger empty vesicles. it is supposed that the contents of these vesicles penetrate ...1978734767
the perineurial and blood-nerve barriers in experimental diabetes.the permeabilities of the blood-nerve barrier and the perineurial barrier were investigated in alloxan and streptozotocin diabetic rats and in the mutant diabetic mouse [c57bl/ks(db/db)]. in the mouse model both fluorescence and electron microscopic techniques were employed using evans blue albumin (eba) and horseradish peroxidase (hrp), respectively, as exogeneous tracers. in the rat models only hrp was used. tracers were applied locally around the sciatic nerve in order to investigate the peri ...1978735757
fate of antigen after intravenous and intraarticular injection into determine the manner in which small protein antigens enter the synovium and are localized, horseradish peroxidase (molecular weight 40,000) was injected by intravenous and intraarticular routes. passage of antigen from capillary lumen to joint space and vice versa occurred primarily across fenestrated vessels. antigen localized to collagen fibers and lining cells. at low concentrations of antigen, antigen was taken up predominantly by marcophages, whereas at higher concentrations both macroph ...1978737015
vascular permeability to proteins and peptides in the mouse pineal gland.the permeability of fenestrated capillaries in the mouse pineal gland to proteins and peptides was demonstrated by means of ultrastructural tracers. horseradish peroxidase (hrp) and microperoxidase (mp) were injected intravenously and allowed to circulate for approximately 30 s, 1 min, 5 min, 1 or 2 h. the tissue was then fixed by vascular perfusion or by immersion with aldehydes. in all experiments a pronounced extravasation of hrp and mp occurred. transendothelial vesicular transport seemed to ...1978737703
lectin analysis of trypanosoma congolense bloodstream trypomastigote and culture procyclic surface saccharides by agglutination and electron microscopic, intact bloodstream trypomastigotes and culture procyclic forms of trypanosoma congolense were tested for aggulination with the lectins concanavalin a (con a), phytohemagglutinin p (pp), wheat germ agglutinin (wga), soybean agglutinin (sba), and fucose binding protein (fbp). similar experiments were conducted with living bloodstream and culture forms treated with trypsin or dextranase. parasites were incubated for 30 min at 25 c in various concentrations of each lectin, then examined for ...1978739411
[auditory field projection into the ventrobasal complex of the thalamus studied by means of horseradish peroxidase and anterograde degeneration]. 1978743907
crossed disynaptic inhibition of sacral motoneurones.1. intracellular recording was made from motoneurones in lower sacral (s2 and s3) segments of the spinal cord in cats, to analyse the neuronal organization of the inhibition evoked in these motoneurones from contralateral afferents. 2. it was confirmed that stimulation of the lowest threshold afferents of contralateral dorsal roots evokes i.p.s.p.s with latencies similar to those of disynaptic i.p.s.p.s. evoked from group ia muscle spindle afferents in limb motoneurones. 3. the crossed disynapti ...1978745104
axonal transport of horseradish peroxidase: a new method for tracing nervous control of the bladder.a new method for tracing the nervous control of the bladder is described. a protein substance, horseradish peroxidase, has been shown to be absorbed and transported by nerve fibers. the substance was injected into the bladder neck region of a series of dogs. after uptake by motor neurons the horseradish was carried to the spinal cord segment controlling micturition. implications regarding the neurophysiology of the urinary tract are discussed.1978746608
permeability of muscle capillaries to interstitially microinjected horseradish peroxidase. 1978748717
[projection of different ganglion cell classes in the albino rat retina. a study using horseradish peroxidase].in order to demonstrate the central projections of the different ganglion cell classes in the albino rat retina horseradish-peroxidase was injected into the superior colliculus (cs) and into the lateral geniculate nucleus (cgl). the study presents the following results: a) injection into the cs: labelled cells are only in the contralateral retina. topistically, the retino-collicular projection is in good agreement with the findings of physiological methods. the nasal half of the retina projects ...1978749383
changes of the stria vascularis induced by ethacrynic acid.histological changes in the stria vascularis of guinea pigs after the injection of ethacrynic acid (50 mg/kg) were investigated using a transmission electron microscope and a scanning electron microscope. the changes in permeability in the stria vascularis was also observed using horseradish peroxidase as a tracer. the pathological changes originating in endothelial cells of stria blood vessels and intermediate cells spread into the marginal cells and basal cells. the most remarkable changes wer ...1978753207
the immune response of the genital tract of the female rabbit following systemic and local immunization.female rabbits were immunized with a model antigen, horseradish peroxidase (hrp), either locally in the reproductive tract or systemically via the intramuscular route. a sensitive immunoassay was developed to detect antibodies to the hrp in both serum and reproductive tract flushings. systemic immunization resulted in circulating and reproductive tract antibodies of the igg class, but none of the iga class. local immunization produced iga and igg antibodies in the reproductive tract, and also ig ...1978756472
organization of trigeminothalamic tracts and other thalamic afferent systems of the brainstem in the rat: presence of gelatinosa neurons with thalamic connections.thalamic projections from trigeminal and certain other nuclei of the brainstem of the rat have been investigated using the technique of retrograde transport of horseradish peroxidase (hrp). the pattern of trigeminothalamic projections is very specifically related to the individual subnuclei of the complex. the main sensory nucleus (msn) provides profuse cross connections to the ventrobasal thalamus (vb); these arise exclusively from medium and small-sized neurons, but never from the large cells. ...1979758332
a preganglionic autonomic nucleus in the dorsal gray commissure of the lumbar spinal cord of the rat.the distribution in the spinal cord of the rat of preganglionic neurons sending fibers into the hypogastric nerve was determined with the retrograde transport of horseradish peroxidase (hrp). labeled cells were present in the intermediate gray matter of spinal segments l1-l2. the majority (81%) of hrp-filled cells formed a continuous column along the midline in the dorsal gray commissure. this cell column was termed the dorsal commissural nucleus (dcn). the remainder of hrp-labeled cells were pr ...1979758335
penicillin effects on cortex synaptic vesicle uptake of horseradish peroxidase. 1979758983
distribution of thalamo-caudate neurons in the cat as demonstrated by horseradish peroxidase.distribution of thalamocaudate neurons in the cat was examined by means of the horseradish peroxidase (hrp) method. after injection of hrp into the head of the caudate nucleus (cd), thalamic neurons labeled with hrp were observed mainly in the rhomboid nucleus (rh), central medial nucleus (ce), centre médian-parafascicular complex (cm-pf) as well as in the midline and intralaminar regions surrounding the mediodorsal nucleus (md). distribution of hrp-labeled neurons in the centrolateral nucleus ( ...1979759222
compartments in the striatum of the cat observed by retrograde cell labeling.the distribution of neurons giving rise to striatal efferent projections has been studied in the cat by the aid of mesulam's histochemical methods following large injections of horseradish peroxidase into the pallidum and substantia nigra. the findings suggest that neurons of medium size form a major fraction of the efferent neurons of the striatum and that these neurons are grouped into geometrically complex, three-dimensional networks.1979759226
ascending projections to the inferior colliculus.cells that send ascending projections to the inferior colliculus were identified following injections of horseradish peroxidase into the colliculus. labelled cells were found in all subcollicular auditory nuclei. virtually all cells of the ipsilateral ventral nucleus of the lateral lemniscus and medial superior olive appear to project to the colliculus. very few cells in these nuclei were labelled on the contralateral side. heavy labelling on the contralateral side was found in the dorsal nucleu ...1979759446
enzymic assay for lecithin in amniotic fluid.we describe an enzymic method for determining lecithin in amniotic fluid. phospholipase d from savoy cabbage is used to liberate choline from lecithin, and the liberated choline is oxidized to betaine and hydrogen peroxide by choline oxidase from arthrobacter globiformis. the hydrogen peroxide forms a colored complex with phenol and 4-aminoantipyrene in the presence of horseradish peroxidase, and this color is measured spectrophotometrically. the method is relatively easy, precise, and accurate, ...1979761344
[morphology of the initial neurons in the cortico-cortical connections between the auditory fields and the sensomotor region revealed by horseradish peroxidase]. 1979761499
the organization of afferents to the cerebellar cortex in the cat: projections from the deep cerebellar nuclei.the topography of the cerebellar nucleo-cortical projection was investigated in the cat by experiments employing the horseradish peroxidase (hrp) technique or by combined hrp-autoradiographic methods. the results of the hrp studies extend previous findings showing that neurons in the deep nuclei project to the cerebellar cortex in an orderly way. thus, it appears that the cortex of the vermis-proper receives projections from neurons located predominately in the fastigial nucleus. intermediate an ...1979762281
sources of subcortical projections to the superior colliculus in the cat.a comprehensive search for subcortical projections to the cat superior colliculus was conducted using the retrograde horseradish peroxidase (hrp) method. over 40 different subcortical structures project to the superior colliculus. the more notable among these are grouped under the following categories. visual structures: ventral lateral geniculate nucleus, parabigeminal nucleus, pretectal area (nucleus of the optic tract, posterior pretectal nucleus, nuclei of the posterior commissure). auditory ...1979762286
a new method for the cytochemical demonstration of peroxidase for light, fluorescence and electron microscopy.a new technique for the cytochemical demonstration of peroxidase is presented. monomeric homovanillic acid is converted by h2o2 and peroxidase into its dimeric form, which is then precipitated as a complex salt of lead and rhodamine 6g or rhodamine b. the reaction product can be visualized by conversion to lead sulphide or viewed directly under the fluorescence microscope, since it emits a red fluorescence when excited with green light. the reaction is rapid and results in good localization at t ...1976768373
[immunofluorescence and immunoelectron microscopic study of changes occurring in secondary reaction of popliteal lymph nodes in young and old rats].a comparative study of the secondary reaction of popliteal lymph nodes in young and adult rats was carried out. in one group of experiments secondary reaction was induced by bovine serum albumin (bsa), in the other one by peroxidase from horseradish. antigen was administered with freund adjuvant. it was established that the index of popliteal lymph nodes in a secondary reaction induced by bsa does not change with the increasing age. the incidence of antibody-producing cells in young and adult ra ...1976781524
distribution of dengue-2 antigens by electron immunocytochemistry.the distribution of dengue-2 antigens was studied in infected monkey kidney cells (llc mk2) using an indirect, horseradish peroxidase-conjugated immunoglobulin technique. this procedure allowed both light and electron microscopic examination of serial-step sections of individual cells cut in a plane perpendicular to the monolayer. both virion and nonvirion antigens were identified on the plasma membrane with this technique. a diffuse cytoplasmic reaction product was also present. the intensity a ...1976786880
detection of endogenous immunoreactive prolactin in rat mammary epithelial cells during lactation.the question of whether endogenous prolactin might be detectable in normally lactating rat mammary glands (lmg) was examined by light microscopic immunohistochemistry. in an initial study, sections of lmg were incubated sequentially with i) rabbit anti-rat prolactin (aprl); ii) anti-rabbit gamma globulin: peroxidase conjugate (argammag-per), prepared from goat anti-rabbit gamma globulin and horseradish peroxidase, and iii) 3,3'-diaminobenzidine mixed with h2o2(dab). use of this three-step proced ...1976789055
cell surface saccharides of trypanosoma lewis i. ii. lectin-mediated agglutination and fine-structure cytochemical detection of lectin-binding sites.bloodstream (bsf) and culture forms (cf) of trypanosoma lewisi were specifically agglutinated with the plant lectins concanavalin a (con a), soybean agglutinin (sba), wheat germ agglutinin (wga), and fucose-binding protein (fbp). lectin-mediated cell agglutination was inhibited, and reversed in the presence of specific lectin-binding saccharides. cells were agglutinated randomly with all lectins suggesting a uniform distribution in the trypanosome cell surface of the lectin-binding saccharide li ...1976789385
characterization of macrophage migration inhibitory factor activity produced in vivo by a cell-mediated immune reaction in the guinea pig.peritoneal fluid from the abdominal cavities of guinea pigs having delayed hypersensitivity to horseradish peroxidase (hrpo) was obtained by a lavage technique before and after i.p. challenge with antigen. macrophage migration inhibitory factor (mif) and macrophage chemotactic factor activities were measured in peritoneal fluids from each animal. chemotactic activity for macrophages was maximal 24 hr after i.p. challenge and was absent thereafter. mif activity was maximal in peritoneal fluid 24 ...1976794416
immuno-electron microscopic localization of immunoglobulins in amyloid fibrils of senile plaques.amyloid fibrils in senile plaques of the brain of patients with senile dementia or alzheimer's disease combined specifically with horseradish peroxidase (hrpo)-labeled rabbit anti-human igg. light and electronmicroscopic immunoperoxidase technique was used to prove this. the fact may mean that immunological factors were involved in the pathogenesis of amyloid fibrils in the senile plaques, and probably also in that of senile dementia or alzheimer's disease.1976795258
trans-epidermal elimination of immune deposits in the skin. an immunohistochemical study on immune deposits using horseradish peroxidase as antigen. 1976798738
possible determinants of the degree of retrograde neuronal labeling with horseradish peroxidase. 1975803395
the efferent connections of the suprachiasmatic nucleus of the hypothalamus.the efferent connections of the suprachiasmatic nucleus of the hypothalamus have been studied in the rat by the injection of 3h-proline into the nucleus and the surrounding regions of the rostral hypothalamus, and by the injection of the enzyme marker, horseradish peroxidase, into the region of the ventromedial hypothalamic nucleus. after an injection of 3h-proline confined to the ventral portion of the suprachiasmatic nucleus, transported label can be followed, in the autoradiographs, dorsally ...1975803516
ocular vascular and epithelial barriers to microperoxidase.microperoxidase (mp) is an ultrastructural tracer of small molecular weight (1,900) derived from horse heart cytochrome c. within the central nervous system, it is capable of entering the periaxonal space which is not open to horseradish peroxidase (hpr). because of its small size and unique behavior, mp was used to probe ocular vascular and epithelial barriers. mp did not enter any ocular space from which hrp was excluded. this tracer established a new lower level of permeability in these ocula ...1975806549
immunochemical studies of the endocrine cells of the gastrointestinal tract. i. the use and value of peroxidase-conjugated antibody techniques for the localization of gastrin-containing cells in the human pyloric antrum.this paper describes and immunochemical localization of gastrin-containing endocrine cells in the human pyloric antrum using antibodies conjugated to horseradish peroxidase. with pre-fixed cryostat sections, a distinct clear-cut staining of gastrin-containing cells can be obtained either by direct or indirect single stain procedures, but may cells containing endogenous peroxidase activity also stain. in order to abolish staining due to endogenous peroxidase, sections were pretreated with a numbe ...1975807542
certain connections of the visual cortex of the monkey shown by the use of horseradish peroxidase. 1975809098
an electron microscopic study of retrograde and orthograde transport of horseradish peroxidase to the lateral geniculate nucleus of the monkey. 1975809099
confirmation of the location of spinothalamic neurons in the cat and monkey by the retrograde transport of horseradish peroxidase. 1975809119
amygdaloid projections to prefrontal granular cortex in rhesus monkey demonstrated with horseradish peroxidase. 1975810217
histochemical characterization of a neocortical projection of the nucleus locus coeruleus in the squirrel monkey.histochemical evidence is presented for a catecholamine-containing projection from the nucleus locus coeruleus to the neocortex in the squirrel monkey. the innervation of superior temporal gyrus has been examined in particular. glyoxylic acid-induced fluorescence shows an extensive arborization of fine, catecholamine-containing fibers with prominent varicosities in all layers of the neocortex. the nucleus locus coeruleus is identified as a source of these fibers by both ortho- and retrograde axo ...1975810499
monkey retinal ganglion cells: morphometric analysis and tracing of axonal projections, with a consideration of the peroxidase technique.this paper presents evidence on the retinal distribution and central projections of retinal ganglion cells of various cell body sizes in the adult macaque monkey. the ganglion cell sizes have been determined by computer assisted measurement of camera lucida drawings at various eccentricities of both flat mounted and sectioned retinae. the pattern of projections of individual ganglion cells to the dorsal lateral geniculate nucleus and superior colliculus has been studied using retrograde axonal t ...1975810500
the origin of efferent pathways from the primary visual cortex, area 17, of the macaque monkey as shown by retrograde transport of horseradish peroxidase.the retrograde transport of horseradish peroxidase has been used to identify efferent cells in area 17 of the macaque. cells projecting to the lateral geniculate nucleus are small to medium sized pyramidal neurons with somata in lamina 6 and the adjacent white matter. the projection to the parvocellular division arises preferentially from the upper half of lamina 6, while that to the magnocellular division arises preferentially from the lower part of the lamina. the projection to both superior c ...1975810501
use of horseradish peroxidase-labelled antiglobulin for the colorimetric quantitation of erythrocyte antibodies.horseradish peroxidase (hrp)-labelled antiglobulin has been used extensively as a histochemical marker. in the method described the stable reaction product formed by using 3,3'-diaminobenzidine (dab) as the hydrogen donor in the peroxidase reaction was dissolved in a fluoro alcohol, 1,1,1,3,3,3-hexafluoroisopropanol (hpf). reproducible standardisation curves resulted with hrp. based on these observations a colorimetric assay procedure was developed to quantitate the amount of antibody coating re ...1975811709
attempts at differentiation of the structure of neurons in the corpus geniculatum laterale of the pig by the immunocytochemical technique.the investigations were carried out on the corpus geniculatum laterale of the pig with the aim to differentiate the structure of its neurons on the basis of the antigenic properties of the nervous tissue. the antigenic material consisted of the homogenate of the corpus geniculatum laterale (h) and of its nuclear (p1) and mitochondrial (p2) fractions. the antigens were used to immunize 3 groups of rabbits, the 4th one serving as control. the antigens were prepared according to requirements of the ...1975812788
blood-aqueous barrier can be circumvented by lowering intraocular pressure.rhesus monkeys were injected intravenously with hypertonic urea (9 ml/kg body weight of 30% urea in 10% invert sugar) and the intraocular pressure was measured with an applamatic tonometer. when this pressure reached its minimum (20% of the normal value) horseradish peroxidase (molecular weight 40,000; radius of an equivalent hydrodynamic sphere about 2.5 nm; 0.5 g/kg body weight), was injected intravenously. twenty minutes following peroxidase administration, either aqueous humor was sampled fr ...1976813231
[demonstration of direct projections between the ponto-mesencephalic tegmentum and the visual cortex of the cat using a peroxidase technique].using a retrograde tracer technique with horseradish peroxidase, we have identified the afferent connections to the visual cortex from the dorsolateral pontine tegmentum (locus coeruleus, nucleus parabrachialis medialis and nucleus parabrachialis lateralis) as well as from the dorsal raphe nucleus.1975813889
structural alterations in the ciliary process and the blood-aqueous barrier of the monkey after systemic urea injections.the structural alterations induced in the monkey ciliary epithelium by intravascular injection of urea solutions were studied with the light and electron microscopes. arterial injection of urea resulted in destruction of the nonpigmented epithelium and the consequent breakdown of the blood-aqueous barrier. the intravenous injection did not significantly affect the structural integrity of the ciliary epithelium. the intercellular zonulae occludens were not altered and the intercellular pathway fr ...1976814818
deoxyribonucleic acid replication in single cells and chromosomes by immunologic techniques.antibodies to 5-bromodeoxyuridine (brdu) or iododeoxyuridine may be used to identify cells or regions of chromosomes in which de novo deoxyribonucleic acid synthesis has occurred. the antibodies to brdu were produced in rabbits by injection of the antigen, a conjugate between bovine serum albumin and bromouridine (bru), or iodouridine. specific antibodies were produced by affinity chromatography on ah-sepharose 4b to which had been coupled bru. anti-bru cross-reacts with iodeodeoxyuridine. indir ...1976815428
midbrain, diencephalic and cortical relationships of the basal nucleus of meynert and associated structures in primates.the structure and connectivity of the basal nucleus of meynert, the substantia innominata in which it lies, and certain related areas have been examined in new world and old world monkeys, using retrograde and anterograde axonal transport methods. experiments using the retrograde, horseradish peroxidase method confirm the observations of kievet and kuypers ('75) that the basal nucleus and substantia innominata project directly, heavily and with a somewhat crude topography upon the neocortex. exp ...1976818134
immunofluorescence and immunoperoxidase observations of anti-lactic dehydrogenase-1 antibody.for immunoelectron-microscopic demonstration of lactic dehydrogenase (ldh), anti-subunit-b antibody was produced in rabbits by immunization with purified pig ldh-1 (b4) isoenzyme. immunoglobin fragment fab' was obtained from anti-ldh serum immunoglobulin g and conjugated with horseradish peroxidase. subunit-b-containing ldh was present diffusely in the sarcoplasm of the pig heart muscle. there was concentration of ldh in the 1-band. ldh reaction was also present in the mitochondrial intermembran ...1976818310
projections from the dorsal lateral geniculate nucleus to prestriate cortex in the squirrel monkey as demonstrated by retrograde transport of horseradish peroxidase. 1976819105
areal and laminar distribution of some pulvinar cortical efferents in rhesus monkey.the areal and laminar distribution of the cortical efferents of the medial, lateral and inferior pulvinar nuclei (pm, pl and pi respectively) were determined in rhesus monkey using autoradiography and horseradish peroxidase (hrp). the autoradiographic data indicated that: areas 8a, 45 and 46 on the convexity and 11 and 12 on the orbital surface of the frontal lobe received projections from pm; areas 20, 21 and 22 in temporal lobe received projections from pm primarily with caudal-medial parts of ...1976823181
spinal projections from the nucleus locus coeruleus and nucleus subcoeruleus in the cat and monkey as demonstrated by the retrograde transport of horseradish peroxidase.the rostral pons of the cat and rhesus monkey were examined for the presence of labeled cells following injections of horseradish peroxidase (hrp) into the lumbar spinal cord. labeled cells were found in the ipsilateral dorsolateral pontine tegmentum and in the contralateral ventrolateral pontine reticular formation. in both the cat and monkey, labeled cells were located in the nucleus locus coeruleus, nucleus subcoeruleus, in or near the kölliker-fuse nucleus, and in the ventral part of the lat ...1976824030
the effect of alloxan on the permeability of isolated pancreatic islets to horseradish peroxidase.the effect of alloxan on the permeability of isolated pancreatic islets to horseradish peroxidase was studied by a perifusion system, which allowed simultaneous monitoring of glucose-induced insulin secretion. rat islets were perifused with a 5 mg/ml glucose solution containing horseradish peroxidase for ten or thirty minutes, with or without prior exposure to alloxan (20 mg%) for five minutes. control islets without alloxan treatment showed few necrotic beta cells diffusely infiltrated with exo ...1976827103
the mammalian rete testis--a morphological examination.the fine structure of the rete testis was examined in several primates, domestic animals and rodents. the rete testis consists of a series of interconnected wide channels lined with a simple cuboidal to columnar epithelium, resting on a thick basal lamina. beneath the basal lamina dense bundles of collagen fibrils and a few blood vessels, lymphatics or nerve tissue are found. the epithelial cells are characterized by large, deeply indented nuclei, spherical or short rod-shaped mitochondria, supr ...1976827216
Displaying items 701 - 800 of 20690