permeability of muscle spindle capillaries and capsule.the permeability of capillaries in rabbit muscle spindles, intramuscular nerves, and extrafusal muscle to intraaortically infused horseradish peroxidase (hrp) was studied. the permeability of the capsule cells of the muscle spindles was also examined. immediately following the infusion of hrp, most extrafusal capillaries contained a dense accumulation of hrp reaction product, which began to escape into the surrounding extracellular space within 2-3 min. in contrast, the concentration of hrp in t ...1979161798
the mechanism of ferrocytochrome c oxidation by a horseradish isoperoxidase.the oxidation of ferrocytochrome c catalysed by highly purified horse-radish isoperoxidase p2 was studied kinetically. to take into account the low turnover number of the enzyme and the tendency to autocatalytic oxidation of ferrocytochrome c, experimental conditions were used which prevented us from using the steady-state treatment. according to kinetic results reported by several authors, a kinetic scheme involving a ternary complex between the enzyme and the substrates was postulated and simu ...1975167872
localization and quantitation of ebv-associated nuclear antigen (ebna) in raji cells.epstein-barr virus (ebv)-associated nuclear antigen (ebna) was localized to chromatin fibers of raji cells. when ebna was labeled with the horseradish-peroxidase technique of sternberger a strong reaction covered most fiber surfaces as observed by electron microscopy. the dry mass of raji chromatin increased after exposure to both ebv-positive and -negative serum, but the increase was significantly larger in the material treated with positive serum.1975168861
the permeability of muscle capillaries to horseradish peroxidase.the main objective of this study was to determine the pathways by which horseradish peroxidase (hrp) can cross the endothelium of muscle capillaries. specimens of mouse diaphragm were fixed for cytochemical analysis at various intervals after intervenous injection of 0.5 mg hrp, at 4 min after intervenous injection of varied amounts of hrp, and at 4 min after intervenous injections in various volumes of isotonic nacl. our findings indicate that endothelial junctions serve as a barrier which ma ...1975169269
cytochemical correlates of structural sexual dimorphism in glandular tissues of the mouse. i. studies of the renal glomerular capsule.the parietal epithelium of bowman's capsule has been analyzed by enzyme cytochemistry in kidneys of mice (c57bl/6j) from birth to 50 days of age. there is a greater tendency for cells in the central portions of the capsular crescent to be cuboidal in post-pubertal males than in pre-pubertal mice of either sex or in post-pubertal females where they are generally squamous; moreover, these heightened capsular cells have a distinct microvillous border. cytochemical procedures were selected which mig ...1975171242
electrical stimulation of the brain. iv. ultrastructural studies.the ultrastructural response of tissues of the cat's central nervous system to chronic electrical stimulation with various parameters has been evaluated at four and a half days post stimulation. cellular alterations included widespread cytoplasmic vacuolations, architectural derangement and disruption of plasmalemma and other membranes. lipid inclusions and dense crystalline bodies were prominent in astrocyte-like phagocytic cells in the more severe lesions. despite endothelial damage in brain p ...1975171783
chicken neutrophils: oxidative metabolism in phagocytic cells devoid of myeloperoxidase.polymorphonuclear leukocytes isolated from chicken peritoneal exudates have been found to catalyze cyanide-insensitive stimulation of respiration and the hexose monophosphate shunt upon exposure to heat-inactivated staphylococcus aureus. however, there was no demonstrable formate oxidation concomitant with phagocytosis in either the presence or absence of exogenous catalase. moreover, chicken polymorphonuclear leukocytes failed to oxidize scopoletin concomitant with phagocytosis in the presence ...1975174090
cellular localization of cyclic amp in periodontal tissues during experimental tooth movement in cats.using an immune-histochemical method, cyclic amp was localized in cells of periodontal tissues in orthodontically-treated cats. sixteen cats were treated for periods ranging from 1 hour to 4 weeks. fresh, frozen, undecalcified 6 mu sections of the tissues were incubated with rabbit anti-cyclic amp antibodies, followed by sequential incubations with sheep anti-rabbit igg, rabbit anti-peroxidase igg and horseradish peroxidase. in the final step, the peroxidase was demonstrated by the diaminobenzid ...1976175896
[further purification and immunohistological localization of human placental lactogen in syncytiotrophoblasts (author's transl)].in 1936, ehrhadt reported a prolactin-like substance which has been identified as a second protein hormone exists in extracted substance from the placenta. to date various reports have been published, but ther are still unsolved problems concerning the specifity of the substance, and its clinical significance is unclear. the purpose of this project was to purify and investigate the biochemical, immunological properties of hpl, and try to localize hpl secretion immunohistologically. the biologica ...1976176070
studies on the chlorinating activity of myeloperoxidase.two methods were utilized to demonstrate the peroxidation of chloride ion to a free species (hocl or cl2) by myeloperoxidase. the peroxidase caused the volatilization of radioactivity from soultions containing hydrogen peroxide and [36cl]nacl, and catalyzed the formation of hocl when solutions contianing these components were passed through a millipore filter to which the peroxidase was adsorbed. in this flow system, 90 mug of canine myeloperoxidase generated 80 mum hocl in the presence of 200 m ...1976176150
use of horseradish peroxidase to study the antagonism of clostridium welchii (cl. perfringens) type d epsilon toxin in mice by the formalinized epsilon prototoxin. 1976176185
subcellular localization of b apoprotein of plasma lipoproteins in rat liver.multispecific antigen-binding fragments (fab) from rabbit antisera against rat very low density lipoproteins (vldl) and fab against rat low density lipoproteins that were monospecific for the b apoprotein were conjugated to horseradish peroxidase. conjugates were incubated with 6-mum frozen sections from fresh and perfusion-fixed livers and with tissue chopper sections (40 mum thick) from perfusion-fixed livers. in the light microscope, specific reaction product was present in all hepatocytes of ...1976177430
peroxidase-conjugate chromatography isolation of conjugates prepared with glutaraldehyde or periodate using polyacrylamide-agarose gel.fractionation abilities of polyacrylamide-agarose gel (ultrogel) and dextran gel (sephadex) column chromatography were compared in isolating horseradish peroxidase conjugates, prepared using two different methods. utrogel aca-44 provides an efficient separation of monomer conjugated and nonconjugated immunoglobulins resulting from the two-step glutaraldehyde procedure, sephadex g-200 does not. both types of columns eluted the polymer conjugates resulting from the periodate procedure in the void ...1976177698
secretion-related uptake of horseradish peroxidase in neurohypophysial axons.during secretion of the neurohypophysial hormones, oxytocin and vasopressin, secretory granule membrane is added to the plasma membrane of the axon terminals. it is generally assumed that subsequent internalization of this additional membrane occurs by endocytosis. in order to study this process, we have traced the uptake of intravenously injected horseradish peroxidase by neurohypophysial axons in rats and golden hamsters. peroxidase reaction product within the secretory axons was found mainly ...1976181385
direct immunoperoxidase staining for regan isoenzyme of alkaline phosphatase in human tumor tissues.immunoperoxidase staining for regan isoenzyme of alkaline phosphatase was performed on cryostat sections of five human tumor tisssues. with a direct immunoperoxidase staining for the localization of regan isoenzyme at the light and electron microscope levels, sections previously fixed with 0.05 m phosphate-buffered 4% paraformaldehyde were reacted with rabbit antisera to human placenta alkaline phosphatase conjugated to horseradish peroxidase. comparison of conventional histochemistry and immuno ...1976182352
early events in polyoma virus infection: attachment, penetration, and nuclear entry.the plaque-assay technique was used as a tool to determine the optimal conditions for adsorption of polyoma virions to host cells. using these optimal conditions of adsorption, an electron microscopy study of the early events of infection was performed. by electron microscopy and autoradiography, it was demonstrated that both the viral coat proteins and dna arrive simultaneously in the nucleus as early as 15 min postinfection. when horseradish peroxidase-labeled virions, pseudovirions, and capsi ...1976183018
peroxidase and fluorescein isothiocyanate as antibody markers. a quantitative comparison of two peroxidase conjugates prepared with glutaraldehyde or periodate anda fluorescein conjugate.batches of rabbit anti-human immunoglobulin g antibodies were labeled either with horseradish peroxidase, using the two-step glutaraldehyde method or the periodate method, or with fluorescein isothiocyanate (fitc). the peroxidase conjugates were isolated by chromatography using two different gel types. the five types of conjugates thus obtained were standardized to the same amount of rabbit immunoglobulin g. the antibody activity, as estimated by means of single radial immunodiffusion and passiv ...1976184204
beneficial effect of catalase treatment on growth of clostridium perfringens.several common plating media were tested for their ability to support growth of clostridium perfringens after storage of the plates for 1 to 10 days at 4 and 25 degrees c with and without subsequent addition of catalase. liver-veal (lv) agar and brain heart infusion (bhi) agar quickly become incapable of supporting growth after storage without added catalase, whereas shahidi ferguson perfringens (sfp) agar and brewer anaerobic (ba) agar were less affected. plate counts of c. perfringens on untre ...1976185958
hydrogen peroxide production and killing of staphylococcus aureus by human polymorphonuclear leukocytes.the effects of bacterial neuraminidase on production of hydrogen peroxide (h2o2) and killing of staphylococcus aureus by human polymorphonuclear leukocytes (pmn) were studied. the concentration of h2o2 was measured by the disappearance of scopoletin fluorescence in the presence of horseradish peroxidase. the results indicated that desialylation of human pmn inhibited the stimulation of h2o2 production during phagocytosis. it also markedly impaired the killing of s. aureus. impaired killing of s. ...1977189859
relay cell classes in the lateral geniculate nucleus of the cat and the effects of visual deprivation.this study presents evidence that the x- and y-cells described physiologically in the a laminae of the cat's dorsal lateral geniculate nucleus (lgn) are two morphologically distinct cell types recognizable in golgi preparations. it is shown firstly that the three cell types seen in golgi preparations of the a laminae (large and medium-sized principal cells and small interneurons-types 1,2 and 3 in the classification of guillery, '66) may be identified in 1-mum epon sections of osmicated material ...1977190274
tissue-specific antibodies against human lung and breast carcinoma dehistonized chromatins.tissue-specific antisera against human lung and breast carcinoma dehistonized chromatins were obtained. the specificity of these antisera was determined by complement fixation. in the presence of antiserum against human lung carcinoma, only chromatins from lung carcinoma fixed complement significantly, whereas chromatins isolated from human breast carcinoma, hela cells, normal lung tissue, breast tissue, or term placenta were negative (i.e., inactive). in a similar assay with the use of antiseru ...1977195066
solid-phase enzyme immunoassay for immunoglobulin m antibodies to cytomegalovirus.a sensitive, solid-phase enzyme immunoassay for the detection of immunoglobulin m antibodies to cytomegalovirus is described. the results of the enzyme immunoassay correlated well with those obtained by an indirect immunofluorescence method. horseradish peroxidase proved to be a more sensitive label than alkaline phosphatase. nonspecific reactions, occurring with commercially available cytomegalovirus antigens, could be avoided by using a nuclear antigen prepared from sonically disrupted nuclei ...1977195977
protein a-peroxidase: a valluable tool for the localization of antigens.protein a of staphylococcus aureus has been conjugated to horseradish peroxidase and used in an indirect immunolabeling technique to visualize membrane and viral antigens. the same protein a-peroxidase conjugate was used with antisera from five different species. using this indirect test, membrane markers for t and b lymphocytes were labeled with a greater specificity than when peroxidase conjugated anti-immunoglobulin was used in the second step. viral antigens on cells infected with measles, v ...1977199666
rat plasma clearance of horseradish peroxidase and yeast invertase is mediated by specific recognition. 1978202501
intracellular demonstration of growth hormone in human mammary carcinoma cells.intracellular growth hormone has been demonstrated in human mammary carcinoma cells by using an unlabeled antibody enzyme technic with horseradish peroxidase and antihorseradish peroxidase complex. intense reaction was seen in both male and female mammary carcinoma cells. positive staining was observed in nuclei and cytoplasm and on cytoplasmic membranes.1978204210
intracellular horseradish peroxidase injection for correlation of light and electron microscopic anatomy with synaptic physiology of cultured mouse spinal cord neurons.synaptic interactions between spinal cord neurons grown in dissociated cell culture were studied electrophysiologically, and presynaptic cells were subsequently injected by intracellular iontophoresis with horseradish peroxidase (hrp). following histochemical processing, injected cells were filled with dense reaction product which facilitated the light and electron microscopic identification of the individual physiologically typed neurons. this technique applied to neurons in monolayer culture a ...1978209872
["functional and anatomical organization of associative" cortical areas 7, 21 and 22 in monkeys. a study of the cortical projections from the pulvinar by means of hrp tracing technique (author's transl)].the thalamic projections to the parietal and temporal associative areas (brodmann's areas 7, 21 and 22) have been revealed by means of the retrograde axonal transport of horseradish peroxidase (hrp). the medial pulvinar (pm) was found to send afferents to the three studied areas. labelled neurones of the pm appeared to be grouped in terms of their cortical target; indeed, neurones of origin of projection fibres to areas 7, 21 and 22 were respectively localized in the dorso-lateral, ventro-latera ...1978211571
comparison of an enzyme-linked immunosorbent assay for quantitation of rotavirus antibodies with complement fixation in an epidemiological survey.the development of a micro-scale enzyme-linked immunosorbent assay (elisa) with horseradish peroxidase as the marker enzyme for the detection and measurement of human rotavirus antibodies is described. a semipurified preparation of the serologically related simian agent, sa-11 virus, was used as the antigen. test sera were reacted with antigen-sensitized wells in disposable poly-vinyl microplates. any attached antibody was detected by the addition of peroxidase-labeled anti-species immunoglobuli ...1978212451
anatomical relationships between the ventral mesencephalic tegmentum--a 10 region and the locus coeruleus as demonstrated by anterograde and retrograde tracing techniques.we have searched for anatomical connections between the ventral mesencephalic tegmentum (vmt), including the dopaminergic cell group a10 and the locus coeruleus (lc) region. tritiated leucine (120--220 nl) and horseradish peroxidase delivered by electrophoresis were injected in the vmt-a10 region. we have demonstrated, on the one hand bilateral projections from the vmt-a10 region to lc, and on the other hand a possible contralateral projection from lc to vmt-a10 region. these relationships, repo ...1979220380
contribution of the locus coeruleus to the adrenergic innervation of the rat spinal cord: a biochemical study.the possible existence and magnitude of a noradrenergic innervation from the locus coeruleus (lc) to the spinal cord was investigated in the rat with various techniques. horseradish peroxidase, injected into the lumbar spinal cord produced heavy labelling of presumably noradrenaline (na)-containing neurons in the ventral region of the lc, while cells in the dorsal region of the lc were only lightly labelled. the effects of electrothermic destruction and electrical stimulation of the lc on levels ...1979220381
[horseradish peroxidase-labeled cells as sources of the spinoreticular and spinothalamic systems of the brain].the cells of spinoreticular and spinothalamic fibrous systems of the cat brain were studied by the method of axone transmission of horse-radish peroxidase (hp). a dense accumulation of hp-labeled neurons establishing direct relations with the reticular formation and thalamus was seen in the upper segments of the spinal cord. in the lower segments these zones were confined to the medial part of the ventral horn and the intermediate zone of the gray matter. the neurons established direct connectio ...1979222379
some aspects of the organization of the lateral geniculate nucleus in galago senegalensis revealed by using horseradish peroxidase to label relay neurons.following injection of horseradish peroxidase into area 17 of the prosimian galago senegalensis, columns of labeled neurons are seen in the dorsal lateral geniculate nucleus extending through all cell layers. individual counts of the number of labeled and unlabeled neurons reveal that from 91 to 98% of all neurons within these densely labeled columns are labeled. these results indicate that most of the neurons within the lgn of the bushbaby project to striate cortex. average diameter measurement ...1979226217
raphe projections to the locus coeruleus in the rat.afferent projections to the locus coeruleus from the various raphe nuclei, particularly of the midbrain (nuclei raphe dorsalis and medianus) and pons (nuclei raphe pontis and magnus), have been studied in the rat by retrograde transport methods using horseradish peroxidase (hrp). the locus coeruleus, in both its dorsomedial and ventrolateral divisions, and in its various anterior-posterior components, were injected with 0.05 microliters of horseradish peroxidase following which various structure ...1979226233
tegmentoreticular projections with special reference to the muscular atonia during paradoxical sleep in the cat: an hrp study.using the retrograde tracer technique with horseradish peroxidase (hrp), attempts were made to determine the cells of origin and the descending pathway of the tegmentoreticular projections in order to give an anatomical substrate for the physiological phenomenon of the postural atonia observed during paradoxical sleep (ps) in the cat. the hrp was injected into various parts of the pontomedullary reticular formation (rf) including the caudal raphe nuclei, nucleus (n.) reticularis gigantocellulari ...1979227527
periodate or glutaraldehyde for preparing peroxidase conjugates?conjugates of horseradish peroxidase with antibodies (anti-human igg (h + l)) or their fab' fragments were prepared according to the newest modification of the periodate (p-) method or the two-step glutaraldehyde (g-) method. the conjugates were analysed by gel chromatography and subsequently tested in three different applications. for tissue immunohistochemistry on sections of lupus erythematosus skin, g-conjugates were preferred to polymeric p-conjugates. in elisa for detection of human antibo ...1979227964
resonance raman study on yeast cytochrome c peroxidase. effect of coordination and axial ligands.resonance raman spectra are reported for native ferric cytochrome c peroxidase, its cyanide and fluoride compounds, those of the ferrous enzyme and its cyanide and carbonyl compounds, and the spectrum of the hydrogen peroxide compound, compound i. band frequencies of ferric horseradish peroxidase isoenzyme c2 and its derivatives are also given. comparison of the frequencies of the bands around 1400, 1500, 1560-1580, and 1610-1640 cm-1 with those of other hemoproteins and heme model compounds sho ...1979228743
enzyme-linked protein a: an enzyme-linked immunosorbent assay reagent for detection of human immunoglobulin g and virus-specific antibody.a general-purpose reagent capable of reacting with immunoglobulin g in a modified enzyme-linked immunosorbent assay technique was prepared by using protein a coupled with horseradish peroxidase. the reagent detected low levels (0.003 to 1.0 microgram/ml) of human immunoglobulin g and was also applied in an enzyme-linked immunosorbent assay for titration of antibody to human cytomegalovirus. the antibody titers to human cytomegalovirus determined by enzyme-linked immunosorbent assay and by comple ...1979231048
[purification of horseradish peroxidase conjugated igg by affinity chromatography. comparison of glutaraldehyde and periodate conjugation].by affinity chromatography of the coupling mixture on concanavalin-a-sepharose unlabeled igg is completely removed. hrp conjugated igg is then separated from free hrp by gelfiltration. glutaraldehyde conjugation yielded 20 to 68% unlabeled igg, depending on the duration of glutaraldehyde addition, and periodate conjugation yielded 10 to 28% unlabeled igg. by this method fractions of conjugates with 3fold spec. act. were obtained by the glutaraldehydemethod and with 1.6fold spec. act. by periodat ...1979232345
the interaction of 5-hydroxytryptamine and related hydroxyindoles with horseradish and mammalian peroxidase systems. 1975234231
california encephalitis virus development in mosquitoes as revealed by transmission studies, immunoperoxidase staining, and electron microscopy.isolates of the snowshoe hare subtype of california encephalitis (ce) virus from yukon mosquitoes during 1972 and 1973 were transmitted by bites of aedes aegypti mosquitoes after 4 to 5 weeks of extrinsic incubation at 55 degrees f after intrathoracic injection, and the 1973 strain was transmitted after mosquitoes were fed virus and held for 3 to 4 weeks at 75 degrees f. antigen of a 1971 isolate of ce virus (marsh lake 23) was detected in salivary glands of infected mosquitoes by the immunopero ...1975235355
endocytosis and multivesicular body formation in rabbit luteal cells during pseudopregnancy.horseradish peroxidase (hrp) was injected directly into the corpora lutea of rabbits on different days of pseudopregnancy. young luteal cells have also been incubated "in vitro" in a medium containing horseradish peroxidase. in the "in vivo" experiments the 5 to 9 days old luteal cells take up far more horseradish peroxidase than the older ones (14-20 days). different types of endocytic vacuoles, mvb's and also some db's are already peroxidase positive shortly (15-20 min) after injection of the ...1975240507
trigeminal nerve and temporomandibular joint of the cat: a horseradish peroxidase study. 1979262237
identification of synaptic acetylcholine receptor sites in retina with peroxidase-labeled alpha-bungarotoxin-horseradish peroxidase conjugate, which binds specificity to nicotinic acetylcholine receptors, was synthesized. this conjugate was bound by 5-7% of the synapses in the inner plexiform layer of the chicken retina. bipolar ribbon synapses as well as amacrine synapses bound the conjugate.1977269390
conjugation of poly-l-lysine to albumin and horseradish peroxidase: a novel method of enhancing the cellular uptake of proteins.the carbodiimide-catalyzed conjugation of a 6700 molecular weight fragment of poly-l-lysine to radiolabeled human serum albumin or to horseradish peroxidase enhances the membrane transport of each protein into cultured mouse fibroblasts approximately 11- and 200-fold, respectively. at least 50% of the peroxidase activity remained after conjugation. trypsinization and carbamylation of the two conjugates demonstrates that the enhancement of their cellular uptake is related to their poly-l-lysine c ...1978273916
ultrastructural observations of horseradish peroxidase in synovial lining cells of the rats' temporomandibular joint. 1977274462
evidence for receptor-mediated binding of glycoproteins, glycoconjugates, and lysosomal glycosidases by alveolar macrophages.alveolar macrophages have been shown to bind glycoproteins and synthetic glycoconjugates (neoglycorpoteins) that have mannose, n-acetylglucosamine, or glucose in the exposed, nonreducing position. galactose-terminal glycoproteins are not bound. binding of radiolabeled ligands to cells is nearly completely impaired by the presence of an excess of yeast mannan. binding is temperature sensitive and proceeds optimally at ph 7.0. prior treatment of the cells with trypsin severely decreases their capa ...1978274729
endocytosis of cholera toxin into neuronal gerl.cholera toxin linked covalently by glutaraldehyde to horseradish peroxidase was incubated with cultured chicken sympathetic neurons at 4 degrees. cells were washed and brought to 37 degrees to permit endocytosis of bound toxin on plasma membranes. massive internalization of the ligand into vesicles and cisterns of the golgi--endoplasmic reticulum--lysosome (gerl) system was demonstrated by the cytochemical reaction for the enzyme. surface binding and subsequent endocytosis of the cholera toxin-- ...1978275852
effect of photocoagulation on the barrier function of the pigment epithelium. ii. a study by electron microscopy.the healing of retinal photocoagulation burns and their permeability to colloidal carbon and horseradish peroxidase was studied by electron microscopy. recent burns showed disruption of intercellular junctions in the pigment epithelium. by 2 days there was infiltration of the burn by proliferating pigment epithelial cells, and by 7 days the burn was composed of a mass of interconnected cells of pigment epithelial and müller cell origin. fresh photocoagulation burns result in complete disruption ...1977281795
modification of the blood-brain barrier: increased concentration and fate of enzymes entering the brain.the blood-brain barrier of rats was opened reversibly by infusing a hyperosmotic solution of arabinose into the external carotid artery. permeability was increased maximally in the first 15 min and remained slightly elevated at 1 hr. osmotic barrier opening significantly increased brain uptake of intravenously injected alpha-mannosidase (alpha-d-mannoside mannohydrolase, ec (derived from human placenta) and horseradish peroxidase (donor:hydrogen-peroxide oxidoreductase, ec ...1979284363
the effect of serotonin on the blood-brain barrier to proteins.since increased concentration of serotonin (5-ht) has been demonstrated in areas of the brain exposed to ischemia and lesions, and since the elevation might be responsible for the enhanced permeability to proteins across cerebral vessels, studies were carried out to elucidate the effect of the amine, perfused through the cerebral ventricular system, on the transport of horseradish peroxidase (hrp) from blood to brain. the amounts of 5-ht were large (50--800 microgram per mouse). the permeability ...1978290745
effect of prostaglandin e2 on the permeability of the iris vessels to horseradish peroxidase in the rabbit.prostaglandin e2 (0.5 microgram in 10 microliter 10% ethanol) was introduced into the anterior chambers of rabbit eyes. using the horseradish peroxidase method, it was shown under the electron microscope that the endothelial barrier of the iris vessels broke down. the peroxidase penetrated as far as the basis of the posterior epithelial cells, however, without entering their lateral intercellular spaces. the question of whether the effect of prostaglandins on the barrier was a direct effect or a ...1978309739
distribution of anti-hrp antibodies in the central nervous system of immunized rats after disruption of the blood brain barrier.lewis rats were immunized with horseradish peroxidase (hrp) injected intradermally in the hind foot pads; anti-hrp antibodies in the cerebrum were detected with a cytochemical reaction for hrp in sections incubated with the antigen (hrp), or after intravenous injection of hrp a few minutes before sacrifice. in two groups of animals the blood brain barrier was disrupted by a heat lesion or by a traumatic lesion (insertion of a canula into the lateral ventricle). in animals with a heat or with a t ...1979313978
rapid stimulation of pinocytosis in human carcinoma cells a-431 by epidermal growth factor.horseradish peroxidase (hrp) uptake was used to measure fluid-phase pinocytosis in monolayers of human epithelioid carcinoma cells (a-431). histochemistry confirmed that cell-associated hrp was restricted to intracellular vesicles. biochemical methods showed that hrp uptake in control cultures was directly proportional to the duration of exposure. the addition of low concentrations of epidermal growth factor (egf) to the incubation media produced a 10-fold increase in the initial rate of pinocyt ...1979315944
retrograde transport of horseradish peroxidase in sensory and adrenergic neurons following injection into the anterior eye chamber.horseradish peroxidase (hrp) was injected into the anterior eye chamber of rats and mice and frozen sections from both superior cervical and trigeminal ganglia were incubated to demonstrate neurons accumulating the tracer by retrograde axonal transport. labelled cells were observed only in ganglia ipsilateral to the hrp injection. within the trigeminal ganglion, peroxidase-containing neurons were restricted to the medial ophthalmic area, whereas labelled cells in the superior cervical ganglion w ...1979317295
caudate afferents from the retrorubral nucleus and other midbrain areas in the order to clarify which midbrain areas project to the caudate nucleus (cd), horseradish peroxidase (hrp) was deposited in the cd of 13 cats. midbrain sections were reacted with diaminobenzidine and sometimes with cobaltous chloride to intensify the reaction, and then examined for retrograde labelling. afferents to the cd were found arising from the following ipsilateral midbrain areas: pars compacta, pars reticulata, and pars lateralis of the substantia nigra (sn), ventral tegmental area of ts ...1978318187
the functional capacity of guinea pig megakaryocytes. ii. the uptake of particles and macromolecules and the effect of rabbit antiguinea pig platelet antiserum.the capacity of megakaryocytes to take up particles and macromolecules was tested by exposing them to homologous erythrocytes, latex particles, micrococcus lysodeikticus, and horseradish peroxidase. uptake of particles 1.1 to 7 mum. in size was extensive under the experimental conditions employed. after uptake of each of the three particles the addition of tannic acid and calcium to the fixative produced electron-dense deposits within the vacuole surrounding the particle; therefore, the vacuole ...1977320386
an ultrastructure study on antibody production of the lymph nodes of rats with special reference to the role of germinal centers.antibody-containing cells in lymph nodes, expecially in germinal centers, and in thoracic duct of rats sensitized by injection of horseradish peroxidase were studied with the immunoperoxidase method. in the primary response the large germinal center cells began to produce antibody on the 9th day and predominated thereafter in germinal centers ,added by intercellular antibody deposition in its late stage. the "ordinary" medium-sized germinal center cells were a minor component. in the secondary r ...1977322442
intimal healing. the pattern of reendothelialization and intimal thickening.studies were undertaken to investigate further the basis of intimal proliferation and the identity of the surface lining cell in rabbit aortas subjected to extensive deendothelialization. endothelial cells were selectively removed by passage of an inflated balloon catheter through the arterial lumen. the healing response was evaluated at intervals up to 36 weeks by several techniques: 1) permeability to evans blue, 2) reappearance of endothelial cells as indicated by the specific marker, adsorbe ...1977322503
solid-phase enzyme immunoassay for hepatitis b surface antigen.a solid-phase enzyme immunoassay is described for measuring hepatitis b surface antigen in human serum or plasma. immunologically purified antibody labeled with horseradish peroxidase was used as the indicator. in the assay system, antibody-coated controlled-pore glass is used as a solid support and there are three sequential incubations, totaling 2 h, at room temperature. results for serially diluted positive and reference sera compare favorably to radioimmunoassay in sensitivity and specifici ...1977322892
ultrastructural immunocytochemical localisation of lysozyme in human bronchial immunocytochemical unlabelled antibody method using rabbit antihuman lysozyme, antirabbit immunoglobulin, and soluble rabbit antihorseradish peroxidase/horseradish peroxidase complexes was used to study the fine structural distribution of lysozyme in human bronchial glands. none was identified in mucous cells but there was heavy staining of the serous cell granules. the serous cell granules were not stained uniformly, suggesting the presence of other secretory products but lysozyme secretion ...1977325682
the localization of proteoglycans and glycoproteins in the hyaline cartilage.antibodies to proteoglycan (pg) and glycoprotein of bovine nasal cartilage were conjugated with fluorescein isothiocyanate and with horseradish peroxidase. hyaluronidase digestion of cartilage tissue-specimens increased the intensity of immune reactions; pronase digestion or extraction with 4 m guanidinium chloride abolished the staining. in the intercellular matrix fine filaments beaded with small granules were seen forming an irregular network. the interstices of the network are filled with co ...1977332674
immune complex receptors on cell surfaces. iii. topography of macrophage receptors demonstrated by new scanning electron microscopic peroxidase marker.receptors for immune complexes have been localized on rabbit alveolar macrophages with scanning electron microscopy by exposing the cells first to a soluble immune complex composed of horseradish peroxidase and antibody to horseradish peroxidase, and then incubating with a benzidine-containing substrate that yields crystalline reaction product. receptors were visualized by this means as sites of attachment of laminated slender crystals that were easily distinguished from macrophage surface struc ...1977333019
evaluation of some of the parameters of the enzyme-linked immunospecific an attempt to standardize the procedure for the enzyme-linked immunospecific assay, several parameters were examined. it was determined that horseradish peroxidase was the enzyme of choice and that several brands of polystyrene tubes and plates could be used. the test was considerably shortened by omitting long incubation steps previously used for eliminating background fixation of conjugate. the reproducibility of the procedure proved to be excellent, but reactivity curves indicated that use ...1977333043
a specific enzymeimmunoassay for progesterone in human enzymeimmunoassay for plasma progesterone was established using progesterone covalently linked to the enzyme, horseradish peroxidase, as the 'label'. separation of free and bound steroid was effected by sepharose-coupled antiprogesterone-11alpha-hemisuccinyl bovine serum albumin antiserum (sepharose-antisera). the enzymeimmunoassay satisfied the normal criteria of specificity, precision and accuracy. comparison of assay results obtained by radioimmunoassay (with and without thin-layer chromat ...1977333666
the blood-brain barrier to horseradish peroxidase under normal and experimental conditions.this review paper deals with the transport of the protein tracer horseradish peroxidase across cerebral vessels under normal and various experimental conditions. electronmicroscopical investigations have revealed that, under normal conditions, a minor vesicular transfer of intravenously injected peroxidase occurs across the endothelium in segments of arterioles, capillaries and venules, especially in arterioles with a diameter about 15-30 mu. this normally occurring vesicular transport is suscep ...1977333857
development of an enzyme-linked receptor assay (era) for enzyme-linked receptor assay (era) for hcg was developed using horseradish peroxidase. the 1,500 g pellets, interstitial cell fraction, and solubilized homogenate from the rat testis linked to tanned sheep red blood cells (srbc) were used as the binding fractions for hcg. in these era systems, the limit of detection for hcg was 30 iu/l (3-12 miu/tube), which was almost equal to that of radio receptor assay (rra). the era using srbc linked solubilized receptor fraction showed the most satisfac ...1977334520
uptake of horseradish peroxidase by geniculo-cortical axons in the golden hamster: analysis by computer reconstruction.micro-injections of horseradish peroxidase (hrp) were made into the visual cortex of the golden hamster. the "projection lines" of labelled neurons in the dorsal lateral geniculate nucleus (lgnd) were three-dimensionally reconstructed, using a computer graphics technique. the lines run rostrally and medially from their origins at the lateral surface of the nucleus. using an anatomically determined retinotopic map of the lgnd, the positions of all labelled cells near the lateral surface were conv ...1977334561
studies on translocation of immunoglobulins across intestinal epithelium. iv. evidence for binding of iga and igm to secretory component in intestinal epithelium.we conducted studies concerning the issue of whether secretory component (sc) is a specific receptor on intestinal epithelial cells for iga and igm. initially, frozen sections of human intestinal mucosa were incubated with dimeric monoclonal human iga, conjugated to horseradish peroxidase; adjacent sections were reacted with peroxidase-conjugated antibodies to sc. the conjugated iga and anti-sc bound to similar sites in the epithelium, that is to basolateral margins and supranuclear cytoplasm of ...1977334623
absorption of horseradish peroxidase by neonatal pig intestinal epithelium: effect of escherichia coli (055b5) on absorption.the pathway of macromolecular transport through the neonatal pig small intestinal epithelium was examined, utilizing the cytochemical marker horseradish peroxidase (hrp). the marker was found adsorbed to the apical microvillous of the enterocytes, within apical tubules, and cytoplasmic vacuoles. extracellular absorbed marker assumed a spherical appearance within the subepithelial spaces but was dispersed within the capillary lumens. uptake of hrp into the enterocyte occurred in 48-hour old neona ...1977335926
enzyme immunoassay for hepatitis b and its comparison to other methods.a solid-phase enzyme immunoassay for the qualitative detection of hepatitis b surface antigenemia is presented and the results compared to the counter-immunoelectrophoresis and radioimmunoassay methods for detection of the same antigen. the enzyme-antibody conjugate was prepared from horseradish peroxidase coupled with antibodies to three sub-types of hepatitis b virus. polystyrene plastic tubes were used as the solid-phase support. the results of the enzyme immunoassay compare favorably with ra ...1977336244
binding of antibodies to acetylcholine receptors in electrophorus and torpedo electroplax membranes.antisera against purified acetylcholine receptors from the electric tissues of torpedo californica and of electrophorus electricus were raised in rabbits. the antisera contain antibodies which bind to both autologous and heterologous receptors in solution as shown by an immunoprecipitation assay. antibodies in both types of antisera bind specifically to the postjunctional membrane on the innervated surface of the intact electroplax from electrophorus electric tissue as demonstrated by an indirec ...1978344325
the projection of the superior colliculus upon the inferior olivary complex of the cat: an autoradiographic and horseradish peroxidase study. 1978346175
organization of corticospinal neurons in the cat.the cells of origin of the corticospinal tract of the cat were identified using the retrograde horseradish peroxidase (hrp) labeling technique. cortical neurons labeled from the spinal cord were confined to layer v and included large betz cells, as well as many smaller neurons of this layer. collections of 5-10 labeled neurons concentrated in areas of 300-500 micrometer diameter were observed, suggesting a columnar-type of organization of corticospinal neurons. injections of hrp into different s ...1978348267
the central location of the motor neurons to the stapedius muscle in the two cats and 10 newborn kittens, retrograde transport of horseradish peroxidase was used to locate the motor neurons of the stapedius muscle. following injection of tracer into the muscle, labeled neurons were observed in the interface between the facial nerve nucleus and the caudal end of the lateral superior olivary nucleus. some marked neurons were also located dorsal and ventral to the facial nucleus. the number of labelled cells varied from a low of 34 to a high of 199.1978348268
immunocytological localization of estrogen in human mammary carcinoma cells by horseradish--anti-horseradish peroxidase complex.tissues from 25 female and 2 male patients of mammary carcinoma were examined by the horseradish--anti-horseradish peroxidase complex to localize the presence of estrogen. carcinoma cells of 10 females and 2 male patients showed positive staining by this technique. the nucleus and cytoplasmic membrane showed the reaction. this method is useful in identifying the exact location of the hormones or hormone-like substances in the malignant cells and, perhaps, will be of great help in selecting the p ...1978349272
brain stem neurons projecting to neocortex: a hrp study in the cat.the cortical projections of the brain stem were investigated in detail in the cat by means of the horseradish peroxidase (hrp) retrograde axonal transport. most of the cells providing ascending fibers to the neocortex were located in the pons (locus coerulus and related structures, central gray substance, dorsal tegmental nucleus, raphe nuclei, reticular nuclei); labeled neurons were also identified in the mesencephalon, mainly in the periaqueductal gray and in the nucleus linearis rostralis. th ...1978350597
cerebellar afferent projections from the vestibular nuclei in the cat: an experimental study with the method of retrograde axonal transport of horseradish peroxidase.details of cerebellar afferent projections from the vestibular nuclei were investigated by the method of retrograde axonal transport of horseradish peroxidase (hrp) in the cat. the distribution of labeled cells in the vestibular nuclei following hrp injections in various parts of the cerebellum indicates that vestibular neurons in the medial and descending nuclei and cell groups f and x project bilaterally to the entire cerebellar vermis, the flocculus, the fastigal nucleus and the anterior and ...1978350598
characterization of various nervous tissues of the chick embryos through responses to chronic application and immunocytochemistry of beta-bungarotoxin.beta-bungarotoxin (beta-bt) was applied to chick embryos at 3-day intervals beginning on the fourth day of incubation to investigate ultrastructurally the effects of chronically and massively applied beta-bt on various nervous tissues and muscles. on the twenty-first day of incubation, spinal cords of beta-bt treated embryos were conspicuously decreased in size. ventral root fibers dorsal root fibers, white matter, and motor neurons disappeared. although spinal ganglia and sympathetic trunk gang ...1978350914
histochemical differentiation of complex carbohydrates with variants of the concanavalin a-horseradish peroxidase method.various treatments carried out prior to the concanavalin a-horseradish perioxidase (hrp) method have been found to affect the staining and have permitted differentiation of three main classes of complex carbohydrates in the rat alimentary tract. class i mucosubstances lose and class ii and iii paradoxically gain concanavalin a-horseradish peroxidase reactivity after periodate oxidation. class ii mucosubstances lose whereas class iii retain or increase their reactivity with a reduction step inter ...1978351046
a morphologic and permeability study of luminal smooth muscle cells after arterial injury in the the carotid air drying model of arterial endothelial injury in the stressed rat, endothelium does not always regenerate over the zone of intimal thickening; instead, a layer of modified smooth muscle cells appears to form a temporary luminal surface. we examined the properties of these luminal smooth muscle cells in injured right carotid arteries from stressed rats at intervals up to 2 months by light, scanning electron, and transmission electron microscopy. before perfusion fixation, selecte ...1978355725
a simple device for pressure injections of horseradish peroxidase into small central neurones. 1978359104
the defined antigen substrate sphere system with direct immunohistoperoxidase for detection of soluble dengue antigen in sera of patients with dengue hemorrhagic fever.the defined antigen substrate sphere system is a simple method for detecting antigen or antibody in the circulation. the technic is based on the coupling of antigen or antibody with sepharose 4b beads that have been activated by cyanogen bromide. in this study the activated beads were exposed to dengue antigen in the serum from a patient with dengue hemorrhagic fever and then stained with antidengue antibody conjugated with horseradish peroxidase. the positive reaction showed brown beads by ligh ...1978360820
enzyme immunoassay for total oestrogens in pregnancy plasma or serum.we have developed an enzyme immunoassay for total oestrogens in pregnancy serum of plasma, using horseradish peroxidase (hrp) as the marker enzyme. a test combination consisting of an antiserum against oestriol-16/17-monosuccinyl-albumin and oestriol-16/17-monosuccinyl-hrp yielded a sensitive system, which reacted to approximately the same extent with oestrone, oestradiol, oestriol and their 16- and 17-conjugates. samples had to be diluted 1 to 10 to avoid interference of plasma factors with the ...1978361293
incorporation of rat histocompatibility (agb) antigens into liposomes, and their susceptibility to immune lysis.the purification of detergent-solubilized rat histocompatibility antigens is described. the antigen may readily be incorporated into synthetic vesicles (liposomes) which appear to be unilamellar and between 0.1 microbeter and 0.2 micrometer in diameter. the addition of specific antibody leads to the agglutination and precipitation of the liposomes. an enzyme, horseradish peroxidase, may be incorporated into the trapped aqueous phase of the liposome, and its release by antibody and complement can ...1978361419
an improved enzyme immunoassay for progesterone in human plasma.we describe an enzyme immunoassay for progesterone in which we use a progesterone-11alpha-hemisuccinyl-horseradish peroxidase conjugate as the "label" and an antiserum raised in rabbits to a progesterone-11alpha-hemisuccinyl-bovine serum albumin conjugate. in this assay, antibody-bound and free steroid are separated by using a second antibody precipitation procedure. the assay has a lower limit of sensitivity of 10 pg/assay tube and satisfies the usual criteria of specificity, precision, and acc ...1978363304
effect of histamine and methacholine on guinea pig tracheal permeability to hrp.the effects of histamine, methacholine, and ether on the permeability of the respiratory mucosa to macromolecules were investigated employing a radioimmunoassay and histochemical techniques to monitor movement of horseradish peroxidase (hrp) from airway lumen to blood. we found that 0.08% of the dose of hrp instilled into guinea pig tracheas was present in the blood volume at 10 min, and plasma hrp levels increased at a rate of 0.0036% instilled dose/min thereafter. after inhalation challenge, s ...1978365840
enzyme-immunoassay of human placental enzyme-immunoassay (eia) for estimation of human placental lactogen (hpl) in plasma or serum was developed using hpl labelled with horseradish peroxidase (hrp) and anti-hpl sera raised in rabbits. the separation between antiserum-bound and free labelled hormone was accomplished with a double antibody solid phase technique. interference of substances from the sample with the immunoassay was prevented by dilution of the sample with at least a factor of 20 and measurement of the labelled hormone ...1979367637
comparison of light-microscopic methods for visualizing horseradish peroxidase granules in stained and unstained tissue.this study was directed toward a comparison of the effectiveness of three different light-microscopical methods for identifying and studying the features of cells that contain horseradish peroxidase (hrp) granules. the cells studied were located in the trigeminal ganglia and were labeled after the injection of hrp solutions into single, maxillary, canine teeth. the tissue was examined with light field, dark field, and differential interference optical systems. the results indicated that differen ...1978368347
electron-microscopic demonstration of streptococcus mutans in 5-day-old experimental dental plaque using immunohistochemistry.the present work described the distribution of horseradish peroxidase reaction product obtained after immunohistochemical staining of consecutive ultra-thin sections of 5-day-old experimental dental plaques using differently absorbed anti-s.mutans sobrinus, anti-s.mutans mutans, and normal rabbit lambda-globulins. it was found that an in situ identification of s.mutans subspecies sobrinus could be obtained using thoroughly absorbed anti-s.mutans sobrinus gamma-globulins and consecutive sections. ...1978369295
luminescence immunoassay (lia): a solid-phase immunoassay monitored by chemiluminescence.a luminescence immunoassay (lia) has been developed utilizing the chemiluminescent luminol reaction with heme as catalyst. rabbit antibody against human serum albumin was quantitated in antigen coated plastic tubes using commercially available goat anti-rabbit igg conjugated to horseradish peroxidase which was the source of heme. the measurable range of antibody is considerably wider by lia than by enzyme immunoassays. the time to develop and measure activity is short and constant which makes li ...1979370310
[thalamocortical projections of the anterior ventral nucleus: study using retrograde axonal transport of horseradish peroxidase]. 1978371568
detection of antibody-forming cells and humoral antibody to horseradish peroxidase.two new simple methods for detecting antibody-forming cells by hemolytic plaque assay and hemagglutinating antibody to horseradish peroxidase have been developed in mice. both techniques utilize as target, sheep erythrocytes coupled directly with horseradish peroxidase. these assays are sensitive, antigen-specific and are useful to quantitate both direct and indirect antibody-forming cells and humoral antibodies.1979372446
a sensitive solid phase enzymeimmunoassay for testosterone in plasma and saliva.a sensitive, solid phase enzymeimmunoassay suitable for determining testosterone concentrations in samll aliquots of plasma (20 microliter) and saliva (200 microliter) has been developed. a solid phase antiserum raised against a testosterone-11 alpha-hemisuccinate/bovine serum albumin conjugate was prepared by coupling to cyanogen bromide activated cellulose. the "enzyme label" was a covalently linked testosterone/horseradish peroxidase conjugate. the assay had a lower limit of sensitivity of 4p ...1979375481
solid-phase enzyme immunoassay for serum ferritin.we describe a solid-phase enzyme immunoassay for ferritin in human serum, with use of a horseradish peroxidase-labeled antibody and a highly sensitive chromogen, 2,2'-azino-di(3-ethyl-benzthiazoline-6-sulfonate). the assay requires only 10 microl of serum per assay, relatively less time and labor than other assys for ferritin, and as little as 10 pg of ferritin can be measured. we believe this assay offers a reliable alternative to radioimmunoassay for serum ferritin.1979378465
tamm-horsfall glycoprotein: ultrastructural immunoperoxidase localization in rat kidney.tamm-horsfall urinary glycoprotein (th) is the primary constituent of urinary casts. the intracellular distribution of th in normal rat kidney was determined by immunoelectron microscopy using horseradish peroxidase-labeled antibodies and compared with morphologic localization of th by immunofluorescence and light immunoperoxidase microscopy. electron microscopy revealed an intracellular localization of th restricted to the thick ascending limb of the loop of henle (alh). in this nephron segment ...1979379435
rat cns in experimental chronic serum sickness: integrity of the zonulae occludentes of the choroid plexus epithelium and brain endothelium in experimental chronic serum sickness.the present study is an extensive systematic immunofluorescence and labelled electron microscopic investigation of chronic rat serum sickness brains. it is specifically directed at discerning effects of chronic serum sickness upon the zonulae occludentes of both the choroid plexus epithelium and the intra-cerebral endothelium. although the choroid plexus is a known site of complex entrapment in systemic immune complex disorders, comprehensive immune studies of the cns parenchymal vasculature hav ...1979384287
descending pathways of the nucleus of the optic tract in the rat.the descending pathways of the nucleus of the optic tract (pars lateralis) (notl) in the rat were examined in nauta-gygax impregnated sections and further confirmed by the horseradish peroxidase labeling technique. following stereotactic lesions in the notl, visual cortex, and superior colliculus, projections which orginate in the notl were identified by elimination of the tectofugal and tectopetal fibers of the superior colliculus. the descending fibers from the notl appeared to be organized in ...1979385106
topographical origin and ganglion cell type of the retino-pulvinar projection in the order to examine the pattern of the retino-pulvinar projection in the cat, the existence of which has been recently demonstrated using autoradiographic fiber tracing technique, a small amount of horseradish peroxidase (hrp) was injected into the lateral part of the pulvinar nucleus at various rostocaudal levels. the retrogradely labeled ganglion cells were analyzed in terms of their topographical location and cell size, as seen inretinal whole mounts. the results were compared with those obta ...1979385107
cytochemical localization of lectin labeled vesicles in gerl region of hepatoma ascites cells.the fate of lectin labeled internalized plasma membrane in the ascites tumor form of the chang rat hepatoma growing under in vivo and in vitro conditions was investigated cytochemically. ascites cells were incubated in convanavalin a (con a) and horseradish peroxidase (po), either with or without prior glutaraldehyde fixation and subsequently treated with 3',3-diaminobenzidine. in cells fixed before con-a-po labeling the reaction product was localized as a continuous and even layer upon the exte ...1979385567
competitive enzyme-linked immunoassay for factor viii antigen.we describe a competitive enzyme-linked immunoassay for factor viii antigen. binding of anti-factor viii to solid-phase factor viii antigen is competitively inhibited by the free factor viii antigen that is to be measured. the amount of anti-factor viii bound to solid-phase viii is measured by applying in sequence a heterologous bridging antibody and a soluble antibody/enzyme immune complex. the soluble complex used was rabbit antiperoxidase/horseradish peroxidase. peroxidase activity is inverse ...1979387293
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