ultrastructural localization of concanavalin a in the perfused rat heart.staining of the surface coat of heart capillaries and aorta was achieved by perfusing concanavalin a and phytohemagglutinin coupled to horseradish peroxidase through the rat heart. the binding of these lectins provides evidence for the presence of glycoproteins containing mannose, glucose, and n-acetylgalactosamine residues in the endothelial surface coat of rat aorta and heart capillaries. the binding of concanavalin a to capillary endothelium was further followed with radioautography of 125i-l ...1976957601
an electron microscopic and cytochemical detection of concanavalin a receptors on the cell membrane of leishmania braziliensis guyanensis.promastigotes of leishmania braziliensis quyanensis cultivated in the nnn medium agglutinate with concanavalin a (con a). the protozoon was agglutinated at different concentrations of con a. maximal agglutination was obtained with 150 mug/ml. three types of agglutination were observed: flagellar-flagellar, flagellar-body and body-body. cell surface polysaccharides, glycoproteins or glycolipids were demonstrated using the periodic acid-thiosemicarbazide-silver proteinate technic. a con a-horserad ...1976961000
reversible blood-brain barrier dysfunction to peroxidase after a small stab wound in the rat cerebral cortex.small stab wounds were made in the rat frontal lobe. the animals were injected with horseradish peroxidase intravenously at different times after the injury in order to study the extravasation of this tracer. there was a leakage of peroxidase into the brain during the first 3 days after the injury. the route of passage from the vessel lumen into the brain was through disrupted blood vessels in the injured region. endothelial pinocytosis and formation of thin, trans-endothelial channel-like struc ...1976961383
cytological observations of the ovarian epithelium in mammals during the reproductive cycle.we have studied the ovarian epithelijm at various stages of the reproductive cycle in a number of mammalian species utilizing light microscopy, scanning microscopy, the freeze-fracture technique, transmission microscopy and by employing specialized tracers that use lanthanum and horseradish peroxidase. we found that the epithelial cells are joined by incomplete tight junctions, gap junctions, and desmosomes. the cytoplasmic matrix contains a large irregularly shaped nucleus, few microtubules, mi ...1976966286
crush injury to peripheral nerve. an electron microscope study employing horseradish peroxidase.electron microscope observations were made of rat peroneal nerve after crushing using intravenously injected horseradish peroxidase (hrp) as a tracer protein to indicate changes in vascular permeability. at 1/2 h and 2 d after the crush there was gross leakage of hrp from damaged capillaries at the site of injury but none from vessels above or below this. ultrastructurally vessels at the site of crush showed broken and separated endothelial cells. proximally and distally there was little abnorma ...1976970115
cells of origin of entorhinal cortical afferents to the hippocampus and fascia dentata of the rat.the pathway from the entorhinal cortical region to the hippocampal formation has previously been shown to be comprised of two sub-systems, one of which projects predominantly to the ipsilateral fascia dentata and regio inferior of the hippocampus proper, and a second which projects bilaterally to regio superior. the goal of the present investigation was to determine if these two pathways might originate from different cell populations within the entorhinal area. the cells of origin of these ento ...1976972204
uptake and transport of exogenous proteins by respiratory epithelium.the tracer proteins, horseradish peroxidase and ferritin, placed in the trachea of guinea pigs were taken up by epithelial cells and transported to the extracellular space. the interval between the introduction of the tracer proteins into the lumen of the trachea and the morphologic demonstration of the porteins in the extracellular space or within the basal portion of the cells was between 30 and 60 minutes. the proteins were transported in vesicles and no penetration of the epithelial intercel ...1976979161
reinnervation of dentate gyrus by homologous afferents following entorhinal cortical lesions in adult rats.granule cells of the rat dentate gyrus which are denervated by unilateral destruction of the entorhinal cortex are reinnervated in part by proliferation of surviving pathways from the contralateral entorhinal cortex. the cells of origin of these lesion-induced projections were identified by retrograde labeling with horseradish peroxidase and were the same cell type which normally project to the ipsilateral dentate gyrus1976982024
ige-induced respiratory and circulatory changes during systemic anaphylaxis in the rabbit.we studied systemic anaphylaxis induced by the administration of 200 mug of horseradish peroxidase into 11 anesthetized rabbits known to be producing anti-horseradish peroxidase antibodies only of the ige class. ventilatory changes included a transient, abrupt decrease in breathing frequency followed by increased minute ventilation; lung mechanical changes included decreased dynamic lung compliance and increased total pulmonary resistance; cardiovascular changes included pulmonary hypertension, ...1976984586
uptake of microperoxidase by segmenting rat ova in vitro.uptake and incidence of microperoxidase (sigma) in the rat ova during cleavage at the 1-, 2-, 8-cell and blastocyst stages were studied after 30 min incubation with the enzyme (molecular weight of 1,900 and size of molecule of 2 nm). evidence was furnished of the presence of a small amount of reaction product of microperoxidase in the zona pellucida and its local concentration in some parts of the perivitelline space. the larger part of surface of the ovum, however, was free of microperoxidase. ...1976986379
quantitative ultrastructural autoradiographic studies of iodinated plasma membranes of lymphocytes during segregation and internalization of surface immunoglobulins.rat spleen lymphocytes were iodinated (125 i) with lactoperoxidase. quantitative autoradiographic studies on cells fixed immediately after iodination showed 19-24% of intracytoplasmic grains at 3hd and over from the plasma membrane. normalization of grain density distribution and comparison of resulting curves with the universal curve of grain scatter of 125 i showed that a significant percentage of intracytoplasmic grains (36%) originates from intracytoplasmic labeled sources rather than from s ...1976986396
peroxidase-antiperoxidase complex. binding by mallory bodies in unfixed antigen-antibody complex of horseradish peroxidase, peroxidase-antiperoxidase (pap), was found to selectively bind to mallory bodies (mbs). specific pap binding to mbs was consistently demonstrated in liver sections from 14 patients with alcoholic cirrhosis and from one patient with hepatoma. mallory bodies in isolated fractions also bound pap. no structures stained with pap in six control sections. the pap-binding method is useful in the identification of mbs in situ and in isolated fraction ...1976989279
the use of tritiated horseradish peroxidase for defining neuronal pathways: a new application. 1976990928
the precise localization of nigral afferents in the rat as determined by a retrograde tracing technique.afferent innervation of the rat substantia nigra (sn) was studied by the retrograde horseradish peroxidase (hrp) method. high concentrations of hrp were deposited in discrete subregions of the sn by means of a microiontophoretic delivery system. using this technique it was possible to demonstrate that the caudatonigral projection system is arranged topographically; all portions of the caudate-putamen except for a central medial core were found to contain hrp positive cells, indicative of retrogr ...1976990939
axonal projections of spinal interneurones excited by group i afferents in the cat, revealed by intracellular staining with horseradish peroxidase. 1976990947
intracellular staining of purkinje cells and their axons with horseradish peroxidase. 1976990950
lymphocyte emigration from high endothelial venules in rat lymph nodes.sequential events during lymphocyte emigration from high endothelial venuses (hev) were studied by scanning and transmission electron microscopy combined with regional perfusion techniques. the results indicate that blood lymphocytes selectively adhere to hev surfaces through microvilli which attach to shallow pits on the luminal surfaces of high endothelial cells. these intercellular contact points resist hydrodynamic and osmotic shearing forces, but can be disrupted by treatments which remove ...1976992709
pinocytic rates of macrophages from mice immunized against toxoplasma gondii and macrophages stimulated to inhibit toxoplasma in vitro.the rate of pinocytosis by macrophages when measured by uptake of horseradish peroxidase was significantly reduced during toxoplasma infection of the cells in vitro when the macrophages were from toxoplasma-immune mice and when control cells were stimulated in vitro to inhibit toxoplasma multiplication. there was, however, no direct correlation between reduced pinocytosis in this model and inhibition or enhancement of toxoplasma multiplication. we conclude that a reduced pinocytic rate is a feat ...1976992866
a histochemical and morphologic study of postobstructive diuresis in the rat.renal functional abnormalities constituting the syndrome of postobstructive diuresis imply both altered tubular and glomerular membrane properties. to determine the morphologic and ultrastructural correlates of this disorder a rat model was developed and 32 postobstructed kidneys were studied by light and electron microscopy at the midpoint of diuresis and compared to 22 controls. the abnormal morphology was: dilated distal tubules and collecting ducts, isolated proximal and distal tubule cells ...1976992966
time sequence and site of fluid accumulation in experimental neurogenic pulmonary edema.the initial phase of pulmonary edema development following intracranial pressure elevation was studied by means of transmission electron microscopy. using perfusion fixation and application of a blood tracer (hrp horseradish peroxidase) the time sequence and site of fluid leakage out of pulmonary vessels was demonstrated: - passage of edema fluid through intercellular clefts of alveolar capillary endothelium - edema accumulation in alveolar interstitial tissue - draining of edema fluid from the ...1976996374
electron microscopic cytochemical localization of intravenously injected liposome-encapsulated horseradish peroxidase in rat liver cells. 1976998408
kinetics of horseradish peroxidase migration through cerebral cortex.this study provides a semiquantitative description of the migration of the extracellular space marker, horseradish peroxidase (hrp), through the cerebral cortex. following a continuous subarachnoid infusion of hrp, this marker was fixed rapidly within the cortical extracellular space (ecs) by intravascular aldehyde perfusion fixation. microscopic measurements of the maximum depth of penetration of hrp into the cortex perpendicular to the pial surface were taken from coronal whole brain sections ...19761000288
cytochemical localization of glycoconjugate in mitochondria.complex carbohydrates and cations have been localized by cytochemical methods in mitochondria of mammalian leukocytes, hepatocytes and oyster gill epithelium. glycoconjugate of acidic nature was visualized with the dialyzed iron method in or on the outer membrane and inner boundary membrane and in the outer intermembrane space but not on the membranes of cristae or in the intracristate space, or matrix. sulfated glycoconjugate was demonstrated with the high iron diamine method in a similar distr ...19761002972
arterial hypotension induced by horseradish peroxidase in various rat strains.the influence of various commercial preparations of horseradish peroxidase upon the arterial pressure has been investigated using direct intra-arterial pressure recordings in nembutal anesthetized rats of different strains. a marked hypotension was noted in wistar and sprague-dawley rats when sufficiently high doses (5-10 mg/100 g body weight) were injected intravenously. this effect was observed with both highly purified (sigma type vi, boehringer reinheitsgrad i) as well as less pure preparati ...19761002975
cerebrovasculature permeability changes following experimental cerebral angiography. a light- and electron-microscopic study.morphological alterations of the cerebral vasculature as related to the permeability of plasma proteins and angiographic contrast media following unilateral cerebral angiography were studied. both evans blue albumin and horseradish peroxidase were employed as protein tracers for light and electron microscopy investigation respectively. grey matter regions of the cerebral cortex, cerebellum corpus striatum, hippocampus and midbrain showed the most extensive and consistent leakage of these protein ...19761003253
penetration of protein tracers into the epiretinal portion of the optic nerve in the rabbit nerve.horseradish peroxidase and fluorescein-labelled globulin were injected into the vitreous of adult and 11- to 13-day-old new zealand albino rabbits. both tracers diffused rapidly into the myelinated nerve fibre layer (the "medullary rays"), which extends over a long distance within the retina in these animals. the tracers penetrated also for some distance into the optic nerve beyond the lamina cribosa, where they were incorporated into a large number of cells in the nerve. this finding of a rapid ...19761003255
polysaccharides in lung alveoli.rat lung alveolar surfaces and contents were studied after using concanavalin a as a bifunctional agent to link exposed sugars to horseradish peroxidase, in accordance with a technique developed by bernhard and avrameas ('71). the graham and karnovsky ('69) diaminobenzidine procedure then was used to provide and electron-dense reaction product so as to define the distribution of complex carbohydrates in alveoli. a layer of very dense raction product was intimately associated with the outer leafl ...19761008038
horseradish peroxidase tracing of the lateral habenular-midbrain raphe nuclei connections in the rat.connections of the habenular complex to the nuclei of the midline in the midbrain (interpeduncularis, medianus raphe, and dorsalis raphe) have been studied classically by anterograde degeneration in the monkey, the cat, and marsupials. passing fibers from the medial septal nucleus and lateral preoptic area, however, have also been demonstrated which can complicate interpretation of these results. in this paper the habenular projections were studied in the rat by the retrograde axonal transport o ...19761009445
thalamic projections to visually responsive regions of parietal cortex.thalamic efferents to visually responsive regions of parietal cortex of the cat were investigated by experimental retrograde tracing techniques. two classes of photically evoked potentials recorded from the middle suprasylvian gyrus could be distinguished on the basis of waveform and latency. type 1 responses were recorded from the clare-bishop area at the lateral border of the suprasylvian gyrus and type 2 responses were recorded from association response areas on the crown of the suprasylvian ...19761009447
the development of the isthmo-optic nucleus.the nucleus of origin of centrifugal fibers to the retina (the so-called isthmo-optic nucleus - ion) has been used as a model for the study of the major features of neural development, from the period of cell proliferation until after the formation of its afferent and efferent connections. 3h-thymidine autoradiography has established that in the chick cells of the ion are generated (i.e., become post-mitotic) between the middle of the 5th and the end of the 7th days of incubation. the first-form ...19761016847
[ultrastructural studies on the permeability of the amnion-epithelium for peroxidase and ferritin. contribution on paraplacental metabolism].by means tracer substances (horseradish peroxidase, ferritin) the permeability of human amnion and umbilical cord (2. to 6. lunar month) has been investigated ultrastructurally. the structure of intercellular cleft permits a passiv transfer of the water and electrolytes in both ways. the findings indicated, that the passage of proteins with a molecular weight of peroxidase predominantly takes place through the intercellular clefts less by means of pinocytosis. ferritin particles can not penetrat ...19761020513
[origin of afferent axons in the striate cortex of the albino rat: a study with horseradish peroxidase]. 19761031505
coronary microcirculatory factors and cardiac muscle cell injury.coronary artery ligation with or without reperfusion was carried out in wistar rats to study the role of coronary microcirculatory factors and membrane permeability alteration of cardiac muscle cell in the evolution of cardiac muscle cell injury by using the fine structural extracellular protein tracer, horseradish peroxidase (hrp). the findings were compared with those obtained in noncoronarogenic myocardial injury models following administration of norepinephrine, a pressor, and isoproterenol, ...19761032011
the relationship between storage and secretion of specific antibody by immune lymphoid cells: ultrastructural localization of anti-peroxidase antibodies in plaque-forming cells of the rabbit popliteal lymph node.the ultrastructure of antibody-forming cells (afc) has been studied in the lymph node cell population from rabbits locally immunized with horseradish peroxydase (po) incorporated in complete freund's adjuvant, and the kinetics of afc development followed from day 7 to day 18 after one injection of po. identification of the afc was done by local hemolysis assay, using carboxymethyl cellulose solidifying medium and po-coated sheep erythrocytes. afc were thereafter transferred by micromanipulation ...19761033066
mesencephalic porjections of the rectus lateralis muscle afferents in the cat.responses to passive stretch applied to the rectus lateralis muscle (rl) were recorded with a microelectrode from the mesencephalic nucleus of the fifth nerve (mes v) in cat encéphale isolé preparation. the necessary conditions to attribute the unit response to an eye muscle stretch are the followings: i) respond neither to jaw movements nor to pressure applied to the eye ball: ii) be activated with a short latency, following stretch of the eye muscles; iii) be excited by a light touch applied t ...19751080407
trigeminal representations of the masticatory and extraocular proprioceptors as revealed by horseradish peroxidase retrograde transport.horseradish peroxidase has been injected in the masticatory and extraocular muscles in newborn and adult cats to identify the cells of origin for the muscle endings. labeled motoneurons in the nuclei of the iii, iv, v and vi nerves have been observed. they are the parent cells of the motor terminals taking up the enzyme in the muscle injected. labeled ganglionic cells have been found scattered all along the ipsilateral mesencephalic nucleus of the v nerve after injection of both the jaw closing ...19751081052
uptake of horseradish peroxidase (hrp) by the endothelium of the short-term stored rabbit vitro study of the uptake of horseradish peroxidase (hrp) by the corneal endothelium was carried out on rabbit corneae which were stored in a preserving medium for various periods. the stored corneae were incubated in hrp solution with or without adenosine triphosphate (atp). the number of pinocytotic vesicles decreased as the storage time was prolonged. addition of atp to the hrp solution led to an increase in the pinocytotic vesicles in all storage periods. the pinocytosis in the 12-day sto ...19761083694
interdigitating reticulum cells in the popliteal lymph node of the rat. an ultrastructural and cytochemical study.electronmicroscopic and cytochemical studies were performed to localize interdigitating reticulum cells (idc) in the popliteal lymph node of the rat. the morphological features of the idc of the rat correspond to those described for other species, but also show similarities to normal macrophages in the rat. this is considered to be an argument in favour of the common origin of idc's and macrophages. ultrahistochemical studies with horseradish peroxidase (hrp) reveal no phagocytotic capacity of i ...19761084780
identification of corticotectal cells of the visual cortex of cats by means of horseradish peroxidase. 19751089457
immunoelectron microscopic identification and localization of streptococcus sanguis with peroxidase-labeled antibody: localization of surface antigens in pure indirect method of localizing antigens with horseradish peroxidase-labeled antibody was used to identify and localize surface antigens of streptococcus sanguis at the ultrastructural level. an electron dense layer surrounding the cell wall could be distinguished without any additional electron microscope staining. this labeled layer represents an immune complex consisting of bacterial surface antigens, specific rabbit antisera, and peroxidase-labeled goat anti-rabbit globulins. although with ...19751090524
the pulmonary inflammatory response. cellular events in experimental pulmonary arterial hypersensitivity disease.horseradish peroxidase (hrp) or bovine serum albumin (bsa) were covalently linked to polyacrylamide or agarose beads and were injected into control syrian hamsters and hamsters previously immunized with either hrp or bsa. animals sensitized to soluble antigen and subsequently challenged intravenously with the same antigen immobilized on beads developed an acute focal inflammatory response within 2 to 6 hours after injection. the acute response involved local deposition of igg and complement (bet ...19751092187
ultrastructural visualization of surface carbohydrate structures on mycoplasma membranes by concanavalin a.surface carbohydrates of mycoplasma mycoides var. capri were made visible by the cytochemical staining procedure with concanavalin a, horseradish peroxidase, and diaminobenzidine.19751104592
editorial: the primary glomerular filtration barrier--basement membrane or epithelial slits?in 1961, based on results obtained with the particulate tracer ferritin, farquhar, wissig and palade [15] proposed a functional model for the glomerulus and defined a role for each of its components in the filtration process: a) the basement membrane as the main filter; b) the endothelium as a valve, which by the number and size of its fenestrae, controls access to the filter; c) the epithelium as a monitor which partially recovers proteins that leak through the filter; and d) the mesangium whic ...19751104965
demonstration of immunoglobulin containing deposits in glomerular basement membrane in experimental chronic serum sickness using horseradish peroxidase labelled antiserum.horseradish peroxidase (hrp) labelled antibodies were used in rabbit glomerulonephritis to obtain localization of immunoglobulins in the glomerulus at a light and electron microscope level. better localization of deposits was obtained with peroxidase labelled antiserum than with immunofluorescence at the light microscope level. ultrastructurally, immunoglobulin localization was seen to correspond to the electron dense deposits demonstrated in and on the glomerular basement membrane by convention ...19751106748
the topographical distribution of s-100 and gfa proteins in the adult rat brain: an immunohistochemical study using horseradish peroxidase-labelled antibodies.the cytological and topographical distribution of s-100 and glial fibrillary acidic (gfa) proteins in the adult rat brain has been compared using the horseradish peroxidase-labelled antibody technique. both proteins are present in astrocytes and structures composed of astrocytic processes, namely the glial limitans and the perivascular membranes, but the cytological localization varies between the two proteins. s-100 is found in the nucleus and cytoplasm whereas gfa protein is confined to the cy ...19761107363
morphology of the breakdown of the blood-aqueous barrier in the ciliary processes of the rabbit eye after prostaglandin e2.protaglandin e2, administered topically to the rabbit eye, causes disruption of the blood-aqueous barrier resulting in a large increase in the protein content of the aqueous humor. the route of plasma proteins into the aqueous humor was studied with the electron microscope, using horseradish peroxidase as a protein tracer. the tracer penetrated the tight junctions of the nonpigmented layer of the ciliary epithelium, filling the lateral intercellular clefts and staining the internal limiting mem ...19751110133
chorioretinal diffusion processes following pigment epithelial degeneration.we studied the effect of sodium iodate-induced pigment epithelial degeneration on chorioretinal diffusion processes. rabbits received two intravenous injections of sodium iodate at the retinotoxic dose of 22.5 mg. per kilogram over a six- to eight-hour period. combined horseradish peroxidase tracing technique and electron microscopy were used to compare diffusion processes over a five-week period. ultrastructural examination of retina twenty-four hours after iodate administration revealed pigmen ...19751110139
subcortical afferents to the frontal lobe in the rhesus monkey studied by means of retrograde horseradish peroxidase transport. 19751111842
a combined horseradish peroxidase-autoradiographic investigation of reciprocal connections between superior temporal gyrus and pulvinar in squirrel monkey. 19751111845
permeability properties of the postcapillary high-endothelial venules in lymph nodes of the mouse.the permeability of the postcapillary high-endothelial venules in mice was investigated by light and electron microscopy. experiments with intravenously injected horseradish peroxidase demonstrated a pronounced intercellular passage of this tracer. vesicular transport was not observed. additional experiments with lanthanum and tannic acid showed that no continous tight junctions sealed the intercellular spaces. however, some focal membrane appositions were observed.19751113511
basal forebrain and hypothalamic connection to frontal and parietal cortex in the rhesus monkey.horseradish peroxidase was injected in different parts of the frontal and parietal cortex in 17 rhesus monkeys. in all cases the enzyme was transported retrogradely to neurons in the substantia innominata and hypothalamus as well as in the thalamus. these new findings demonstrate that these cortical areas receive direct afferent fibers from limbic basal forebrain areas concerned with emotion and motivation.19751114317
acquisition of host antigens by young schistosoma mansoni in mice: correlation with failure to bind antibody in vitro.young schistosomes collected after penetration through isolated mouse skin (3 h schistosomula) were cultured in medium containing immune rhesus monkey serum with a high titre of antibody known to kill schistosomula in the presence of complement. morphological signs of damage in electron micrographs were confined to the surface tegument of the schistosomula. antibodies in immune rhesus serum were shown to bind to the surface membrane of 3 h schistosomula using an antibody-enzyme bridge technique ...19751118189
permeability of ovarian follicles and capillaries in mice.the permeability of ovarian capillaries and follicles in prepubertal and sexually mature (proestrus and metestrus) randomly bred swiss albino female mice (sch:ars ha icr strain) was studied by intravenous injection of either ferritin or horseradish peroxidase (hrp), followed by examination with light and electron microscopes. the study revealed that capillaries in the interstitial and perifollicular regions were provided with a continuous endothelium that had constant permeability characteristic ...19751119413
increased penetration of horseradish peroxidase across the blood-brain barrier induced by metrazol seizures. 19751122393
lymphocyte differentiation and antibody synthesis in the secondary immune response of peroxidase stimulated lymph nodes of rat.lymphocyte differentiation and specific antibody synthesis were studied in the course of the secondary immune response of horseradish-peroxidase immunized rat popliteal lymph nodes by electron microscopic immunocytochemistry. from primary immunization, mature plasma cells containing specific antibody remained as long as 10 months. antihorseradish peroxidase antibodies were localized in lymphocytic blast cells on day 3 after restimulation with antigen. in the subsequent days blast cells were stil ...19751122527
an ultrastructural quantitative method for the evaluation of the permeability to horseradish peroxidase of cerebral cortex endothelial cells of the rat.a quantitative method for the evaluation of the importance of pinocytotic phenomena in cerebral cortex capillaries is proposed. the method involved the counting of labeled "caveolae intracellulares" in endothelia cells with the use of horseradish peroxidase as a tracer molecule. a mean pinocytotic value per capillary is then computed. it is demonstrated that the value obtained after irradiation of the brain is higher than in control animals. this information may provide an ultrastructural quanti ...19751122535
h2o2 release from human granulocytes during phagocytosis. i. documentation, quantitation, and some regulating factors.the extinction of fluorescence of scopoletin during its oxidation by horseradish peroxidase (hpo) provides a highly sensitive and specific assay for small quantities of peroxide in solution. with this assay, the release of free h2o2 into the extracellular medium by phagocytizing human granulocytes has been documented and quantitated, and some of the regulating factors have been determined. under basal conditions granulocytes released less than 0.01 nmol/ml of h2o2 (2.5 x 10-6 polymorphonuclear l ...19751123431
application of immunoperoxidase techniques to localize horseradish peroxidase-tracer in the central nervous system.immunoperoxidase techniques are presented which can be used to localize horseradish peroxidase-tracer in paraffin-embedded tissues of the central nervous system. compared to histochemical methods using frozen sections, these immunologic techniques allow the use of stored, serial paraffin sections, and appear more sensitive for the demonstration of intraneuronal horseradish peroxidase after retrograde transport. the immunoperoxidase bridge techniques from reaction products of high quality which c ...19751127219
sympathetic preganglionic neurons of the cat spinal cord: horseradish peroxidase study. 19751131693
diffusion of horseradish peroxidase perfused through the lateral ventricle of the chick telencephalon.horseradish peroxidase, perfused into the lateral ventricle of chick brain, freely and slowly diffuses through the cerebral extracellular spaces. the layer of astrocytic end-feet surrounding blood capillaries does not consitute a barrier to the tracer which permeates the basal lamina, diffuses between the pericytic cells and finally accumulates in the intercellular space beneath the tight junctions between contiguous endothelial cells. no evidence was found for transport by micropinocytotic vesi ...19751131857
effects of contrast agents on the blood-brain barrier. an electron microscopic determine if contrast agents after the blood-brain barrier by opening tight junctions between endothelial cells and by increasing pinocytosis, the distribution of horseradish peroxidase (hrp) was evaluated after intracarotid injections of contrast media. hrp did not cross the endothelium of cerebral blood vessels. tight junctions remained closed. there was increased endothelial cell pinocytosis and swelling of astrocyte end-feet.19751138267
the effect of portocaval anastomosis on the permeability to horseradish peroxidase of cerebral vessels of the rat.the blood-brain barrier to horseradish peroxidase (hrp) was tested in rats with hepatic encephalopathy induced by portocaval anastomosis. an increased number of vesicles containing hrp was seen in endothelial cells. basement membranes of some vessels and the adjacent neuropil were filled with hrp. astrocytes showed swollen end-feet. the cytoplasm of astrocytes adjacent to hrp containing basement membranes was evenly filled with hrp. this phenomenon could be caused either by cellular hyperactivit ...19751146553
some aspects of the organization of the thalamic reticular complex.anatomical methods which depend upon the anterograde axonal transport of isotopically labeled neuronal proteins or the retrograde axonal transport of the enzyme, horseradish peroxidase, have been used to elucidate the relationships between the reticular complex and the dorsal thalamus and cerebral cortex. injections of tritiated amino acids in the dorsal thalamus or cerebral cortex in rats, cats and monkeys, show that as the bundles of thalamo-cortical and cortico-thalamic fibers joining a parti ...19751150923
pathogenesis of acute arterial fat deposition in spontaneously hypertensive rats.the selectively-bred substrains of spontaneously hypertensive rats with a greater vulnerability to vascular lesions rapidly developed arterial fat deposition within 1 or 2 weeks as well as a greater hypercholesterolemic response when fed on high fat cholesterol diet including 20% of suet, 5% of cholesterol and 2% of cholic acid. the ring-like arterial fat deposition at the branches of superior mesenteric arteries and cerebrobasal arteries, which was found to be good indices for the deposition of ...19751152192
evidence for bidirectional dendritic transport of horseradish peroxidase. 19751155260
observations on cortical neurons retrogradely labeled with horseradish peroxidase. 19751155261
the appearance of dendrites of callosal and corticothalamic neurons in somatosensory cortex of immature rats demonstrated by horseradish peroxidase. 19751155262
absorption of intact protein by colonic epithelial cells of the rat.colonic absorption of intact protein was examined in adult rats using histological and ultrastructural procedures. horseradish peroxidase was introduced into ligated colonic loops and retained therein for 5, 10, 20, or 30 minutes prior to excision of the loops, and their processing for microscopy. morphological evaluation revealed evidence of peroxidase absorption via pinocytosis. tracer particles were observed adherent to the mucosal border, in apical pinocytotic vesicles, in vesicles adjacent ...19751155414
demonstration of a somatotopically organized projection onto the paramedian lobule and the anterior lobe from the lateral reticular nucleus: an experimental study with the horseradish peroxidase method.using the retrograde axonal transport of horseradish peroxidase, the projection from the lateral reticular nucleus (nrl) to the cerebellar anterior lobe and paramedian lobule has been studied in 13 cats. both the anterior lobe and the paramedian lobule receive a somatotopically organized projection from the nrl. the projection to the paramedian lobule is nearly exclusively ipsilateral and originates mainly in the dorsal part of nrl, while the projection to the anterior lobe is bilateral (with ip ...19751156873
the pontocerebellar projection onto the paramedian lobule in the cat: an experimental study with the use of horseradish peroxidase as a tracer.horseradish peroxidase (hrp) was injected into cerebellar cortex of the paramedian lobule in 12 cats, and the ensuing distribution of labeled cells in the pontine nuclei was mapped in some detail. the cells in the pontine gray which give origin to fibers to the paramedian lobule lie together, in part in groups, and in part in columns. the columns are situated both medial and ventrolateral to the peduncle, as well as in the dorsolateral pontine nucleus. the projection is bilateral with a clearcut ...19751156877
the projections of different morphological types of ganglion cells in the cat retina.the central projections of the retinal ganglion cells of the cat were examined using the method of retrograde transport of horseradish peroxidase. peroxidase was injected into the lateral geniculate nucleus and into the superior colliculus by means of a recording micropipette. after injections at retinotopically homologous points in these two structures in separate animals, tha patterns of retinal ganglion cell labeling were compared. we found that there were three populations of ganglion cells: ...19751159111
the projections of cells in different layers of the cat's visual cortex.the projection of cells in different layers of several cortical visual areas in the cat were studied using the method of retrograde transport of horseradish peroxidase. injections of the enzyme were made through a recording micropipette, making it possible to localize the injection site by physiological criteria. we found that layer vi cells projected to the alteral geniculate nucleus, while a distinct population of cells in layer v projected to the superior colliculus. cells in layers ii and ii ...19751159112
studies on the structure and permeability of the microvasculature in normal rat lymph nodes.the structure and permeability of the microvasculature in normal rat lymph nodes was studied by regional perfusion techniques. the results indicated that characteristic vascular units supplied each cortical lobule of lymphatic tissue. numerous arteriovenous communications and venous sphincters innervated by unmyelinated nerve fibers were found in this vascular bed. these specialized vascular structures permitted regional control of blood flow through high endothelial venules. lymphocytes migrate ...19751163637
impermeability of sertoli cell junctions to prolonged exposure to peroxidase.the competence of the occluding junctional complexes between sertoli cell has been studied after continuous exposure of the testis to a high concentration of peroxidase for periods of 30 minutes to 4 hours. horseradish peroxidase in buffered saline solution (10 mg/ml) infused through an indwelling polyethelene tube implanted interstitially in the testis becomes widely distributed throughout the organ via the extensive system of lymphatic sinusoids. all the components of the testicular interstiti ...19751163825
macromolecular transport by the middle ear and its lymphatic system.the macromolecular transport in the middle ear mucosa of the guinea pig was investigated by means of light and electron microscopy using tracer substances such as evans blue, india ink, and horseradish peroxidase (hrp). hrp particles were readily taken up by the middle ear mucosa and eustachian tube. they were absorbed by all cell types, including ciliated, secretory and nonsecretory cells. the particles were first taken up by the pinocytotic vesicles and then transported into the intercellular ...19751166775
immunoelectron microscopic identification and localization of streptococcus sanguis with peroxidase-labeled antibody: localization of streptococcus sanguis in intact dental plaque.streptococcus sanguis has been localized ultrastructurally within intact dental plaque by means of an indirect technique which utilizes horseradish peroxidase-labeled antibody. the technique allows for complete diffusion of the reagents to all portions of the plaque specimens. control procedures can be carried out on serial sections of plaque with a bacterial composition similar to that of the experimental specimen. the 30-mum-thick sections can be examined in the light microscope to localize ar ...19751167846
endogenous peroxidase: specific marker enzyme for tissues displaying growth dependency on derived from a correlated morphological and biochemical study suggest the following: (a) estradiol-17beta, diethylstilbestrol, the estrogen antagonists nafoxidine (upjohn 11,000), and parke davis c1628 induce synthesis of an endogenous peroxidase in the epithelium of target tissues like the vagina, the cervix, the uterus, and in the acinar cells of the estrogen-dependent rat mammary tumor; (b) peroxidase is a "specific" secretory protein of the estrogen-sensitized uterine endometrium; (c) p ...19751171106
permeability of rabbit corneal epithelium to horseradish peroxidase after the influence of benzalkonium chloride.a tight barrier against permeation of horseradish peroxidase into the corneal epithelium exists at the corneal surface adjacent to the tear film. the present light and transmission electron microscopic study reveals that the cationic surfactant, benzalkonium chloride, which is commonly added to eye drops as a preservative, breaks down this barrier. lysis of the cell membranes was demonstrated, resulting in a leakage of the tracer into and underneath the superficial cells. the grade of cellular d ...19751174396
magnocellular nuclei of the basal forebrain project to neocortex, brain stem, and olfactory bulb. review of some functional correlates.horseradish peroxidase was injected into the neocortex of squirrel monkeys, rats, tree shrews and one opossum, in the brain stem of one squirrel monkey and rats, and in the olfactory bulb, the corpus vitreum or the vascular system of rats. following the cortical, brain stem and bulbar injections labeled cells were found (predominatly ipsilaterally) in the magnocellular nuclei of the basal forebrain: nucleus of the diagonal band, the magnocellular preoptic nucleus and nucleus basalis. these nucle ...19751174977
delineation of dorsal lateral geniculate afferents from the cat brain stem as visualized by the horseradish peroxidase technique. 19751174981
different targets of different neurons in nucleus gracilis of the cat.efferent projections of neurons in the cat nucleus gracilis to the ventrobasal complex (vb) of the thalamus and the dorsal accessory portion of the inferior olive (iod) were studied using tracing techniques that exploit neuronal orthograde and retrograde intra-axonal transport processes. these projections were studied in order to determine if the heterogeneity of the morphology, physiology and afferent input of the nucleus gracilis is paralleled by a similar heterogeneity in its efferent project ...19751176641
localization of masticatory motoneurons in the cat and rat by means of retrograde axonal transport of horseradish peroxidase.topographical localization of monotoneurons supplying the masticatory muscles was investigated in the cat and rat, utilizing retrograde axonal transport of horseradish peroxidase. following injection of horseradish peroxidase in each masticatory muscle, motoneurons labelled with peroxidase were seen to be aggregated into a cluster within the motor nucleus of the trigeminal nerve. such clusters of peroxidase-motoneurons innervating each masticatory muscle were demarcated more sharply in kittens t ...19751176649
ultrastructural studies on cerebrovascular permeability in acute hypertension.acute hypertension, experimentally induced by intravenous injection of metaraminol in adult rabbits, rapidly induced a damage of the blood-brain barrier in the cerebral cortex, as visualized by evans-blue-conjugated albumin and horseradish peroxidase. extravasation of these two exogenous tracers was demonstrated to occur in arterioles, in capillaries and, rarely, in venules. peroxidase passed the endothelial cell into the nervous tissue in either or three different ways, i.e. through channels, o ...19751180001
mössbauer investigations of high-spin ferrous heme proteins. i. cytochrome p-450.anaerobically reduced samples of cytochrome p-450 from pseudomonas putida were studied by mössbauer spectroscopy. in the presence of an applied magnetic field the high-spin ferrous heme iron showed an intricate pattern of electric and magnetic hyperfine interactions which could be parametrized successfully in terms of a spin hamiltonian formalism. the results imply a very low (triclinic) symmetry of the heme iron. the effects of the ligand environment and of spin-orbit coupling result in a large ...19751182094
mössbauer investigations of high-spin ferrous heme proteins. ii. chloroperoxidase, horseradish peroxidase, and hemoglobin.reduced samples of chloroperoxidase, horseradish peroxidase, and deoxyhemoglobin were studied by mössbauer spectroscopy in strong magnetic fields. the intricate paramagnetic spectra of chloroperoxidase were evaluated in detail in the framework of a spin hamiltonian pertinent to high-spin ferrous iron. the studies strongly suggest that, in their reduced states, chloroperoxidase from caldariomyces fumago and cytochrome p-450 from pseudomonas putida have similar, if not identical ligand structures ...19751182095
vasogenic edema in the injured spinal cord: a method of evaluating the extent of blood-brain barrier alteration to horseradish peroxidase. 19751183533
proceedings: a quantitative study of horseradish peroxidase uptake by boutons presynaptic to axotomized hypoglossal neurones. 19751185647
location of cat trigeminal ganglion cells innervating dental pulp of upper and lower canines studied by retrograde transport of horseradish peroxidase. 19751188971
the central cervical nucleus as cell origin of a spinocerebellar tract arising from the cervical cord: a study in the cat using horseradish peroxidase. 19751192184
cell populations of the lateral geniculate nucleus of the cat as determined with horseradish peroxidase. 19751192198
localization of the efferent neurons of the tensor tympani muscle of the newborn kitten using horseradish peroxidase. 19751193198
altered reabsorption of protein by the renal cortex in rats treated with hypertonic saline or mannitol.the reabsorption of horseradish peroxidase (hrp) by the proximal tubule cells of rat kidneys was investigated by measuring the concentration of hrp in total particulate fractions of the cortex 1/4 and 1 hr after intravenous injection, and by correlated cytochemical observations. when compared to the corresponding values of the control animals, the concentration of hrp 1 hr after injection was decreased approximately 10-fold in the renal cortex of rats which had received an intravenous injection ...19751194661
membrane permeability alterations as manifestation of early cardiac muscle cell injury.studies with extracellular fine structural diffusion protein tracers, horseradish peroxidase and ferritin, were carried out on catecholamine-induced cardiac muscle cell injury. epinephrine, norepinephrine, and isoproterenol were administered in a dose of 4- 6 mug/100g body weight by continuous intravenous infusion. for follow-up studies, isoproterenol was also given subcutaneously in a dose of 8.5 mg/100 g body weight as a single injection. in contrast to saline-infused controls and following ep ...19751197896
changes in vascular permeability in stroke-prone spontaneously hypertensive rats studied with peroxidase as a tracer.cerebrovascular permeability in stroke-prone spontaneously hypertensive rats (shr) at various ages was histologically studied using horseradish peroxidase as a tracer and such was related to the cerebrovascular lesions in the animals. an increase in permeability was demonstrated in the brain of shr, particularly in those animals with an extremely high blood pressure. increased cerebrovascular permeability occurred in some animals without any organic vascular change or severe parenchymal changes, ...19751199727
structural changes in the membrane of vero cells infected with a paramyxovirus.vero cells productively infected with the halle strain of measles virus have been studied by means of surface replication, freeze-fracturing, and surface labeling with horseradish peroxidase-measles antibody conjugate in order to examine changes in the structure of the cell membrane during viral maturation. early in infection, the surfaces of infected cells are embossed by scattered groups of twisted strands, and diffuse patches of label for viral antigens cover regions marked by these strands. ...19751202014
an ultrastructural study of normal and injured hypoglossal nuclei after injection of horseradish peroxidase.the left hypoglossal nuclei of normal rats, and rats which had received left hypoglossal nerve axotomies 7-21 days previously, were studied by electron microscopy after injection of horseradish peroxidase as a marker of extracellular space and pinocytosis. quantitative analysis showed that the number of pinocytotically-derived structures in presynaptic boutons was significantly increased in rats at 7, 14, and 21 days after axotomy when compared with normal rats. it was suggested that presynaptic ...19751204692
[evidence for direct projections from the hypothalamus to the lateral geniculate and occipital cortex of the cat as demonstrated by horseradish peroxidase technique (author's transl)]. 19751204798
olfactory relationships of the telencephalon and diencephalon in the rabbit. ii. an autoradiographic and horseradish peroxidase study of the efferent connections of the anterior olfactory nucleus.the efferent connections of the anterior olfactory nucleus in the female albino rabbit have been studied using the autoradiographic and horseradish peroxidase methods for tracing axonal pathways. following a unilateral injection of 3h-leucine into the olfactory peduncle, radioactively labeled efferent projections from the anterior olfactory nucleus were traced into all layers of the ipsilateral main olfactory bulb beneath the olfactory nerve layer and through the ipsilateral anterior limb of the ...19751206126
the olivocerebellar projection in the cat studied with the method of retrograde axonal transport of horseradish peroxidase.the distribution of labeled cells in the inferior olive of the cat has been mapped following injections of small amounts of horseradish perosidase in the paramedian lobule of the cerebellum. the distribution of labeled cells was plotted in drawings of approximately serial transverse sections. the findings in each case were transferred to a standard diagram of the olive to facilitate comparison of cases. previous studies of the distribution of retrograde cell loss in the inferior olive following ...19751206129
functional and structural changes in mammalian sympathetic neurones following interruption of their axons.the effects of interrupting the axons of principal neurones in the superior cervical ganglion of adult guinea-pigs were studied by means of intracellular recording, and light and electron microscopy. 1. within 72 hr of axon interruption, the amplitude of exitatory postsynaptic potentials potentials (e.p.s.p.s) recorded in principal neurons in response to maximal preganglionic stimulation declined. e.p.s.p.s were maximally reduced (by more than 70% on average) 4-7 days following interruption, and ...19751206535
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