Publications

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arf6 targets recycling vesicles to the plasma membrane: insights from an ultrastructural investigation.we have shown previously that the adp-ribosylation factor (arf)-6 gtpase localizes to the plasma membrane and intracellular endosomal compartments. expression of arf6 mutants perturbs endosomal trafficking and the morphology of the peripheral membrane system. however, another study on the distribution of arf6 in subcellular fractions of chinese hamster ovary (cho) cells suggested that arf6 did not localize to endosomes labeled after 10 min of horseradish peroxidase (hrp) uptake, but instead was ...09456320
translation attenuation via 3' terminal codon usage in bovine csn1s2 is responsible for the difference in αs2- and β-casein profile in milk.the rate of secretion of αs2-casein into bovine milk is approximately 25% of that of β-casein, yet mammary expression of their respective mrna transcripts (csn1s2 and csn2) is not different. our objective was to identify molecular mechanisms that explain the difference in translation efficiency between csn1s2 and csn2. cell-free translational efficiency of csn2 was 5 times that of csn1s2. transcripts of csn1s2 distributed into heavier polysomes than csn2 transcripts, indicating an attenuation of ...025826667
purification and characterization of chorion peroxidase from aedes aegypti eggs.previous study has shown that a peroxidase is present in the mature eggs of aedes aegypti mosquitoes, and the enzyme is involved in the formation of a rigid and insoluble chorion by catalyzing chorion protein crosslinking through dityrosine formation. in this study, chorion peroxidase was solubilized from egg chorion by 1% sds and 2 m urea and purified by various chromatographic techniques. the enzyme has a relative molecular mass of 63,000 as estimated by sds-page. spectral analysis of the enzy ...010871050
oxidations catalysed by horseradish- and milk-peroxidases. 193216744935
factors affecting regeneration of the horseradish root. 194016653625
the action of horseradish-peroxidase on angiotonin, pepsitensin and epinephrine.(1) the pressor action of angiotonin, pepsitensin and epinephrine is rapidly destroyed by the horseradish-peroxidase-hydrogen peroxide system. (2) this reaction is considerably enhanced by the addition of small amounts of potassium iodide.194517730622
[not available]. 194720254343
the enzyme-substrate compounds of horseradish peroxidase and peroxides; kinetics of formation and decomposition of the primary and secondary complexes. 194918131422
the properties of the enzyme-substrate compounds of horseradish peroxidase and peroxides; the reaction of complex ii with ascorbic acid. 194915405728
the properties of the enzyme-substrate compounds of horseradish peroxidase and peroxides; the effect of ph upon the rate of reaction of complex ii with several acceptors and its relation to their oxidation-reduction potential. 194915405729
chemical nature of the secondary hydrogen peroxide compound formed by cytochrome-c peroxidase and horseradish peroxidase. 195214929252
horseradish peroxidase. 195214919613
splitting of horseradish peroxidase into prosthetic group and protein as a means of studying the linkages between hemin and protein. 195214904464
the chemical nature of the second hydrogen peroxide compound formed by cytochrome c peroxidase and horseradish peroxidase. 2. formation and decomposition. 195313093668
the chemical nature of the second hydrogen peroxide compound formed by cytochrome c peroxidase and horseradish peroxidase. i. titration with reducing agents. 195313058869
the third intermediate compound of horseradish peroxidase and hydrogen peroxide. 195313044812
intermediate compounds formed when horseradish peroxidase reacts with potassium molybdicyanide and potassium chloriridate. 195313038479
a simple method for the preparation of horseradish peroxidase. 195413172193
[studies of antibacterial substances from horseradish (cochlearia armoracia), nasturtium (tropaeolum maius) and garden peppergrass (lepidium sativum)]. 195713435755
oxidation-reduction potentials of horseradish peroxidase. 195713416301
[effect of ethereal horseradish oils on mycobacteria tuberculosis]. 195713496904
colorimetric analysis with n, n-dimethyl-p-phenylenediamine of the uptake of intravenously injected horseradish peroxidase by various tissues of the rat.1. a method is described for the colorimetric determination of peroxidase with n,n-dimethyl-p-phenylenediamine. the amount of red pigment formed by peroxidase is proportional to the concentration of enzyme and to the time of incubation during the first 40 to 90 seconds. the influence of the concentration of enzyme, n,n-dimethyl-p-phenylenediamine, h(2)o(2), the time of incubation, ph, the temperature, and the possible interference by oxidizing and reducing agents of tissues has been tested. 2. t ...195813587547
[electron microscopic studies on morphological changes of tubercle bacteria after action of ethereal horseradish oils]. 195813625801
[on the factors influencing the reaction of the horseradish peroxidase-ascorbic acid system]. 195913806578
[treatment of diseases of the respiratory tract with an extract from cochlearia armoracia (horseradish)]. 195913643388
rapid cytochemical identification of phagosomes in various tissues of the rat and their differentiation from mitochondria by the peroxidase method.1. granules characterized by their ability to segregate foreign proteins (phagosomes) were identified in the cells of many rat organs after intravenous administration of horseradish peroxidase, by using the conventional test with benzidine for the histochemical detection of peroxidase. the largest numbers of phagosomes were identified in kidney and liver. considerable numbers were observed cytochemically in pancreas, prostate, epididymis, thymus, spleen, bone marrow, small intestine, heart, pitu ...195913654438
[the antibacterial action of ethereal oils from horseradish root (cochlearia armoracia l.)]. 196013755847
[comparative clinical studies on non-specific urinary tract infections with an enzyme-glycoside mixture obtained from horseradish roots (cochlearia armoracia l.)]. 196013747687
role of bacteria in the oxidation of myoglobin.the addition to steaks of cell suspensions of a number of aerobic bacteria and of saccharomyces cerevisiae greatly increased the rate of discoloration. low inocula resulted in the more rapid appearance of the brown color of metmyoglobin, whereas high cell populations quickly produced the purple color of myoglobin. sonically treated suspensions of pseudomonas geniculata produced similar changes in surface color but less rapidly. no such effect was observed with lactobacillus plantarum.the visible ...196114492670
biosynthesis of mustard oil glucosides. i. administration of c14-labelled compounds to horseradish, nasturtium, and watercress. 196213995362
colorimetric investigation of the uptake of an intravenously injected protein (horseradish peroxidase) by rat kidney and effects of competition by egg white.after intravenous injection of horseradish peroxidase into rats, the foreign protein appeared in the kidney first in the small phagosomes and its concentration there decreased quickly; it then was concentrated and "stored" for several days in the large phagosomes. after injection of 10 mg of peroxidase per 100 gm of body weight, the concentration of peroxidase in blood and urine decreased exponentially during the first 6 hours; small amounts of peroxidase were excreted in the urine for several d ...196213917690
cytochemical investigation of phagosomes and related structures in cryostat sections of the kidney and liver of rats after intravenous administration of horseradish peroxidase. 196213917691
[alteration of bacterial toxins by the constituents of chamomile and horseradish. 4]. 196314208688
the stimulation of horseradish peroxidase by nitrogenous ligands. 196314086725
the oxidatic activity of horseradish peroxidase. ii. participation of ferroperoxidase. 196314109214
the oxidatic activity of horseradish peroxidase. i. oxidation of hydro- and naphthohydroquinones. 196314109213
[alteration of bacterial toxins by the ingredients of camomile and horseradish. 1]. 196314117727
[alteration of bacterial toxins by the constituents of chamomile and horseradish. 3]. 196314210438
the hydroxylation of proline by horseradish peroxidase. 196414249129
biosynthesis of mustard oil glucosides. iv. the administration of methionine-c14 and related compounds to horseradish. 196414209394
[electrophoretic and chromatographic separation of peroxidase in horseradish]. 196414318458
cytochemical observations on the relationship between lysosomes and phagosomes in kidney and liver by combined staining for acid phosphatase and intravenously injected horseradish peroxidase.after incubation of formalin-fixed, frozen sections of kidney and liver from peroxidase-treated rats in an azo dye medium for acid phosphatase, and after subsequent incubation of the same sections with benzidine, phagosomes were stained blue and lysosomes were stained red in the same cells. it was observed that newly formed phagosomes were separate from preexisting lysosomes in the tubule cells of the kidney and in the kupffer cells of the liver at early periods after treatment with peroxidase. ...196414128050
occurrence of phagosomes and phago-lysosomes in different segments of the nephron in relation to the reabsorption, transport, digestion, and extrusion of intravenously injected horseradish peroxidase.the size, number, and location of lysosomes, phagosomes, and phago-lysosomes in different segments of the proximal and distal tubules, in the collecting tubules, and in invading macrophages of the kidneys of rats were compared by staining lysosomes (acid phosphatase) red, and phagosomes (injected horseradish peroxidase) blue in separate sections, and by staining phago-lysosomes purple by successive application of the reactions for the two enzymes in the same sections. it was concluded from these ...196414189907
factors affecting the state of injected horseradish peroxidase in animal tissues and procedures for the study of phagosomes and phago-lysosomes. 196414200765
[study on several systems used for chemiluminescent determination of horseradish peroxidase].four systems were studied used for chemiluminescent determination of hrp and the determination conditions optimized. in luminol-h2o2 (naoh) system, hrp was determined conveniently in the range of 0.05-4 pmol, with a correlation coefficient of 0.998 for the linear log-log equation and a limit of detection of 16 fmol hrp. in pyrogallol-h2o2 (ph 6.5) system, 0.1-100 pmol of hrp were determined, based on three log-log regression equations with correlation coefficients of 0.997, 0.994 and 0.999, resp ...19642093069
the role of horseradish peroxidase in indole-3-acetic acid oxidation. 19655881594
the formation of thyroxine from diiodotyrosine in the presence of horseradish peroxidase and hydrogen peroxide. 19655325809
investigations on the multiple components of commercial horseradish peroxidase. 196514298831
the activity of the horseradish peroxidase compound 3. 196514265755
histochemical studies on the uptake of horseradish peroxidase by rat kidney slices.when rat kidney slices were incubated in the presence of horseradish peroxidase, there was an energy-dependent uptake of the protein by the cells of the kidney tubules. the uptake was greatest in the proximal convoluted tubules and in the thick ascending limbs of the loops of henle; it was abolished by cold, anoxia, 2,4-dinitrophenol, and fluoroacetate, and was more readily depressed by unfavorable metabolic conditions in the proximal convoluted tubules than in the thick ascending limbs. protein ...19655884629
a rapid assay for peroxidase activity.1. peroxidase has been assayed by a chronometric method involving the coupled reaction of ascorbic acid with the product of the enzymic action on benzidine. 2. measurements of the activities of horseradish and tea peroxidase by this and two other methods, involving respectively pyrogallol and o-dianisidine, are compared. 3. it is claimed that the chronometric method is relatively simple, rapid and accurate. 4. the method can be used in the presence of polyphenol oxidases.196616742428
studies on cytochrome c peroxidase. iv. a comparison of peroxide-induced complexes of horseradish and cytochrome c peroxidases. 19665911629
the behavior of horseradish peroxidase at high hydrogen peroxide concentrations. 19665963444
reversible conversion of horseradish paraperoxidase (low spin) into normal peroxidase (high spin) by sulfhydryl reagents. 19665961094
peroxidase isozymes from horseradish roots. i. isolation and physical properties. 19665946638
[contribution to the study of mechanisms of indolylacetic acid degradation by horseradish peroxidase]. 19665940633
glomerular permeability. ultrastructural cytochemical studies using peroxidases as protein tracers.1. glomerular permeability was studied by ultrastructural cytochemistry, using as protein tracers two intravenously injected peroxidases of differing molecular weight. 2. horseradish peroxidase (molecular weight 40,000) passed rapidly through the endothelial fenestrae, across the basement membrane, and through the epithelial slits into the urinary space. human myeloperoxidase (molecular weight 160,000 to 180,000) also passed rapidly through the endothelial fenestrae and across the basement membr ...19665925318
the early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. 19665962951
partial purification and kinetics of indoleacetic acid oxidase from tobacco roots.extracts from roots of nicotiana tabacum l var. bottom special contain oxidative enzymes capable of rapid degradation of indoleacetic acid (iaa) in the presence of mn(2+) and 2, 4-dichlorophenol. purification of iaa oxidase was attempted by means of ammonium sulfate fractionation and elution through a column of se-sephadex. two distinct fractions, both causing rapid oxidation of iaa in the absence of h(2)o(2), were obtained. one fraction exhibited high peroxidase activity when guaiacol was used ...196616656384
reactions of horseradish peroxidase with azide. evidence for a methionine residue at the active site. 19676073036
biosynthesis of ethylene. ethylene formation from methional by horseradish peroxidase. 19676066254
the columnar cells occurring in the parietal layer of bowman's capsule. cellular fine structure and protein transport.the renal corpuscles of adult, c3h swiss, male mice contain testosterone-sensitive, columnar cells in the parietal layer of bowman's capsule. a study of the normal fine structure of these cells reveals several distinctive characteristics: a microvillous brush border; apical tubular invaginations and apical tubules; an elaborate infolding of the basal surface membrane forming cellular compartments, which contain numerous mitochondria; and a complex group of membrane-limited cytoplasmic inclusions ...19674861776
the ultrastructural basis of capillary permeability studied with peroxidase as a tracer.the transendothelial passage of horseradish peroxidase, injected intravenously into mice, was studied at the ultrastructural level in capillaries of cardiac and skeletal muscle. peroxidase appeared to permeate endothelial intercellular clefts and cell junctions. abnormal peroxidase-induced vascular leakage was excluded. neutral lanthanum tracer gave similar results. the endothelial cell junctions were considered to be maculae occludentes, with gaps of about 40 a in width between the maculae, rat ...19676061717
indoleacetic acid oxidase activity of apoperoxidase.the conventional activity of electrophoretically purified horseradish peroxidase toward guaiacol, pyrogallol, 2,6-dimethoxyphenol, and benzidine is abolished by removal of the heme prosthetic group with a mixture of cold acetone and hydrogen chloride. the apoenzyme, though devoid of peroxidase activity, retains its activity as an indoleacetic acid oxidase when it is supplied with 10(-5) mole of manganous ion and 2,4-dachlorophenol per liter. this oxidase activity is cyanide-sensitive; azide also ...19676038167
modification of enzymic activity. 3. effect of salts and organic additives on horseradish peroxidase. 19676078084
the pathway between hyaloid blood and retinal neurons in the toad. structural observations and permeability to tracer substances.the hyaloid vessels form a capillary network on the inner surface of the retina. these capillaries are embedded in the vitreous humor, and they lack a glial investment. the intercellular spaces of the retina communicate with the ocular cavity, as can be evidenced by following the penetration of tracer substances. hence, there is an extracellular diffusion pathway between hyaloid capillaries and retinal neurons, without interposition of glial cells. trypan blue and ferrocyanide were not detected ...19676035649
studies on the equilibria and kinetics of the reactions of peroxidases with ligands. 3. the dissociation of carbon monoxide from carbon monoxide ferro-horseradish peroxidase. 19676049439
fine structural localization of a blood-brain barrier to exogenous peroxidase.horseradish peroxidase was administered to mice by intravenous injection, and its distribution in cerebral cortex studied with a recently available technique for localizing peroxidase with the electron microscope. brains were fixed by either immersion or vascular perfusion 10-60 min after administration of various doses of peroxidase. exogenous peroxidase was localized in the lumina of blood vessels and in some micropinocytotic vesicles within endothelial cells; none was found beyond the vascula ...19676033532
peroxidase isozymes from horseradish roots. ii. catalytic properties. 19676026238
enzyme-labeled antibodies for the light and electron microscopic localization of tissue antigens.enzymes, either acid phosphatase or horseradish peroxidase, were conjugated to antibodies with bifunctional reagents. the conjugates, enzymatically and immunologically active, were employed in the immunohistochemical localization of tissue antigens utilizing the reaction product of the enzymatic reaction as the marker. tissues reacted with acid phosphatase-labeled antibodies directed against basement membrane were stained for the enzyme with gomori's method, and those reacted with peroxidase-lab ...19676046571
analysis of the conditions causing the oscillatory oxidation of reduced nicotinamide-adenine dinucleotide by horseradish peroxidase. 19674291647
changes in intracellular location of small phagosomes (micropinocytic vesicles) in kidney and liver cells in relation to time after injection and dose of horseradish peroxidase. 19676051741
functions of coated vesicles during protein absorption in the rat vas deferens.the role of coated vesicles during the absorption of horseradish peroxidase was investigated in the epithelium of the rat vas deferens by electron microscopy and cytochemistry. peroxidase was introduced into the vas lumen in vivo. tissue was excised at selected intervals, fixed in formaldehyde-glutaraldehyde, sectioned without freezing, incubated in karnovsky's medium, postfixed in oso(4), and processed for electron microscopy. some controls and peroxidase-perfused specimens were incubated with ...19676055991
a histochemical and biochemical study of some aspects of placental function in the rat using maternal injection of horseradish peroxidase. 19676058928
methods for the study of small phagosomes and their relationship to lysosomes with horseradish peroxidase as a "marker protein". 19674167626
vascular leakage induced by horseradish peroxidase in the rat. 19674383959
the effect of ligand binding and acid splitting on the optical rotatory dispersion of ferric horseradish peroxidase. 19685723400
peroxidase isoenzymes from horseradish roots. 3. circular dichroism of isoenzymes and apoisoenzymes. 19685658539
circular dichroism of horseradish peroxidase isoenzymes and apoisoenzymes. ucla-12-686. 19685732289
transformation of horseradish peroxidase into photosensitive compounds in the presence of indole-3-acetic acid. 19685668185
transverse tubular system in glycerol-treated skeletal muscle.horseradish peroxidase used as an extracellular marker fills 98.5 percent of the central triadic elements (tubules) in normal muscle and 97.2 percent in muscle soaked in ringer solution to which 400-millimolar glycerol is added. a glycerol-soaked muscle rapidly returned to normal ringer solution has a disrupted transverse tubular system and has only 3.2 percent of triads filled with peroxidase.19685648264
the ultrastructural basis of alveolar-capillary membrane permeability to peroxidase used as a tracer.the permeability of the alveolar-capillary membrane to a small molecular weight protein, horseradish peroxidase (hrp), was investigated by means of ultrastructural cytochemistry. mice were injected intravenously with hrp and sacrificed at varying intervals. experiments with intranasally instilled hrp were also carried out. the tissue was fixed in formaldehyde-glutaraldehyde fixative. frozen sections were cut, incubated in graham and karnovsky's medium for demonstrating hrp activity, postfixed in ...196811905208
spectral studies of iron coordination in hemeprotein complexes: difference spectroscopy below 250 millimicrons.in order to evaluate the feasibility of observing the spectral behavior of protein groups in the coordination sphere of the iron in hemeproteins, criteria are developed to determine whether or not the application of difference absorption spectroscopy to the study of complex formation will be successful. absolute absorption spectra, 300-1100 mmu, from bacterial catalase complexes are displayed, and the infrared bands correlated with magnetic susceptibility values of similar complexes of other hem ...19685699802
conversion of griseophenone a to (+) or (-)-dehydrogriseofulvin in the presence of horseradish peroxidase and hydrogen peroxide. 19685657554
the electronic structure of protoheme proteins. i. an electron paramagnetic resonance and optical study of horseradish peroxidase and its derivatives. 19685646479
studies on the mechanism of the horseradish peroxidase catalyzed luminescent peroxidation of luminol. 19685648750
the oxidation of horseradish peroxidase by periodate. 19684296876
a comparison of horseradish peroxidase and manganese ions as catalysts for the oxidation of dihydroxyfumaric acid.with horseradish peroxidase as catalyst the main product was dihydroxytartrate, but small amounts of glycolaldehyde, mesoxalic semialdehyde, mesoxalate and possibly glyoxal were also formed. mn(2+) catalysis gave rise only to mesoxalate and oxalate. when oxygen uptake was followed by a manometric method the rate of the peroxidase-catalysed reaction was proportional to oxygen concentration and marked inhibition by cyanide was obtained only at low buffer concentration. the catalytic effects of per ...19685660638
alkylation of horseradish peroxidase with iodoacetate. 19685661605
tetanus toxin: fine structure localization of binding sites in striated muscle.when striated muscle from mice poisoned with purified tetanus neurotoxin was incubated with tetanus antitoxin labeled with horseradish peroxidase and stained for peroxidase activity, an electron-opaque reaction product was found in the transverse and terminal sacs of the longitudinal elements of the sarcotubular system. demonstration of toxin in this location suggests that it acts by interfering with contraction coupling or mechanisms of contraction-relaxation.19685716138
circular dichroism of horseradish peroxidase and its enzyme-substrate compounds. 19685640166
participation of horseradish oxyperoxidase (compound 3) in interenzymic reaction steps. 19685640162
cytochemical detection of sites of antibody to horseradish peroxidase in spleen and lymph nodes. 19684172922
ultrastructural localization of antibody in differentiating plasma cells.antibody was localized by electron microscopy within differentiating and mature plasma cells of the spleens of hyperimmunized rabbits. horseradish peroxidase was used as antigen. intracellular antibody to peroxidase was revealed in glutaraldehyde-fixed tissue by coupling it with its antigen and then revealing the sites of peroxidase activity cytochemically. antibody first appears in the perinuclear space of hemocytoblasts where it persists through differentiation into immature plasma cells, but ...19685635036
ultrastructural studies on the permeability of the mesothelium to horseradish peroxidase.peritoneal mesothelium was exposed for 2-60 min to solutions of horseradish peroxidase by incubation in vitro, or after intraperitoneal injection in vivo. peroxidase was localized, with the electron microscope in the intercellular clefts of the mesothelium, often along their entire lengths, in vesicles adjoining or contiguous with the clefts, and along the peritoneal and basal surfaces of the cell, and also in intracytoplasmic vacuoles. the intercellular junctions of peroxidase-treated mesotheli ...19685645837
resistance of wistar-furth rats to the mast cell-damaging effect of horseradish peroxidase. 19685656148
absorption of horseradish peroxidase by the mucosal cells of the duodenum of mouse. i. the fasting animal. 19685656460
the anatomical organization of septo-hippocampal projections.since the time of elliot smith (1910) it has been recognized that the septal complex occupies a pivotal position within the mammalian telencephalon, being strategically placed between the hippocampal formation on the one hand and the basal forebrain and diencephalon on the other. however, it is only in the last few years that the detailed interrelationships between the different nuclear groups within the septum and the various subfields of the hippocampus have been studied. we have recently re-e ...1968252443
iaa oxidase inhibitors from normal and mutant maize plants.extracts of maize (zea mays l.) plants contain substances which, in vitro, inhibit an indoleacetic acid (iaa) oxidase enzyme from maize. the extracts can be freed of inhibitors by dialysis or by passage through columns of polyvinylpyrrolidone powder. inhibitor-free extracts contain an iaa oxidase enzyme which requires a phenolic co-factor and is stimulated by mn(2+).iaa oxidase inhibitor and total phenol levels were compared for normal maize and for the maize mutant knotted (kn). in plants up to ...196916657240
mössbauer spectroscopic evidence for the electronic configuration of iron in horseradish peroxidase and its peroxide derivatives. 19695346394
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