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sequence variations in the fad2 gene in seeded pumpkins.seeded pumpkins are important economic crops; the seeds contain various unsaturated fatty acids, such as oleic acid and linoleic acid, which are crucial for human and animal nutrition. the fatty acid desaturase-2 (fad2) gene encodes delta-12 desaturase, which converts oleic acid to linoleic acid. however, little is known about sequence variations in fad2 in seeded pumpkins. twenty-seven fad2 clones from 27 accessions of cucurbita moschata, cucurbita maxima, cucurbita pepo, and cucurbita ficifoli ...201526782391
comparison of enzymatic and ultrasonic extraction of albumin from defatted pumpkin (cucurbita pepo)
seed powder.in this study, ultrasound- and enzyme-assisted extractions of albumin (water-soluble protein group) from defatted pumpkin (cucurbita pepo) seed powder were compared. both advanced extraction techniques strongly increased the albumin yield in comparison with conventional extraction. the extraction rate was two times faster in the ultrasonic extraction than in the enzymatic extraction. however, the maximum albumin yield was 16% higher when using enzymatic extraction. functional properties of the p ...201527904383
assessment of heavy metal pollution in vegetables and relationships with soil heavy metal distribution in zhejiang province, china.there are increasing concerns on heavy metal contaminant in soils and vegetables. in this study, we investigated heavy metal pollution in vegetables and the corresponding soils in the main vegetable production regions of zhejiang province, china. a total of 97 vegetable samples and 202 agricultural soil samples were analyzed for the concentrations of cd, pb, as, hg, and cr. the average levels of cd, pb, and cr in vegetable samples [chinese cabbage (brassica campestris spp. pekinensis), pakchoi ( ...201526013654
draft genome sequence of enterobacter aerogenes, a dde-degrading and plant growth-promoting strain isolated from cucurbita pepo.we report here the draft genome of enterobacter aerogenes, a gram-negative bacterium of the enterobacteriaceae isolated from cucurbita pepo root tissue. this bacterium shows 2,2-bis(p-chlorophenyl)-1,1-dichloroethylene (dde)-degrading potential and plant growth-promoting capacity. an analysis of its 4.5-mb draft genome will enhance the understanding of dde degradation pathways and phytoremediation applications for dde-contaminated soils.201525883299
draft genome sequence of methylobacterium radiotolerans, a dde-degrading and plant growth-promoting strain isolated from cucurbita pepo.we announce the draft genome of methylobacterium radiotolerans, a gram-negative bacterium isolated from cucurbita pepo roots. this strain shows 2,2-bis(p-chlorophenyl)-1,1-dichloroethylene (dde)-degrading potential and plant growth-promoting capacities. analyses of its 6.8-mb genome will improve our understanding of dde-degradation pathways and aid in the deployment of phytoremediation technologies to remediate dde-contaminated soils.201525977414
use of video surveillance to measure the influences of habitat management and landscape composition on pollinator visitation and pollen deposition in pumpkin (cucurbita pepo) agroecosystems.pumpkin (cucurbita pepo) production relies on insect-mediated pollination, which is provided by managed and wild pollinators. the goals of this study were to measure the visitation frequency, longevity and temporal activity patterns of pumpkin pollinators and to determine if local habitat management and landscape composition affected this pollination service. we used video surveillance to monitor bee acitivty within male and female pumpkin flowers in 2011 and 2012 across a pollination window of ...201526587337
structure, evolution and functional inference on the mildew locus o (mlo) gene family in three cultivated cucurbitaceae spp.the powdery mildew disease affects thousands of plant species and arguably represents the major fungal threat for many cucurbitaceae crops, including melon (cucumis melo l.), watermelon (citrullus lanatus l.) and zucchini (cucurbita pepo l.). several studies revealed that specific members of the mildew locus o (mlo) gene family act as powdery mildew susceptibility factors. indeed, their inactivation, as the result of gene knock-out or knock-down, is associated with a peculiar form of resistance, ...201526715041
differential uptake and translocation of β-hch and dieldrin by several plant species from hydroponic medium.to compare the uptake and translocation of hydrophobic organic chemicals by plant species, the authors performed uptake experiments with β-1,2,3,4,5,6-hexachlorocyclohexane (β-hch) and 1,2,3,4,10,10-hexachloro-6,7-epoxy-1,4,4a,5,6,7,8,8a-octahydro-endo-1,4-exo-5,8-dimethanonaphthalene (dieldrin) using 5 species: hordeum vulgare, glycine max, solanum lycopersicum, brassica oleracea, and cucurbita pepo. the present study evaluated uptake ability using root concentration factor (rcf) and translocat ...201525470472
developmental biology of zeugodacus cucurbitae (diptera: tephritidae) in three cucurbitaceous hosts at different temperature regimes.fruit flies are key pests of cucurbits in many parts of the world, including tanzania. developmental biology of zeugodacus cucurbitae (coquillett) has been determined across temperature regimes in some cucurbitaceous hosts, in limited geographies. this study was conducted to determine duration and survival rates of immature stages of z. cucurbitae in three cucurbitaceous hosts, at different temperature regimes. it was hypothesized that temperature and cucurbitaceous hosts influence duration and ...201526589874
partially resistant cucurbita pepo showed late onset of the zucchini yellow mosaic virus infection due to rapid activation of defense mechanisms as compared to susceptible cultivar.zucchini yellow mosaic virus (zymv) is an emerging viral pathogen in cucurbit-growing areas wordwide. infection causes significant yield losses in several species of the family cucurbitaceae. to identify proteins potentially involved with resistance toward infection by the severe zymv-h isolate, two cucurbita pepo cultivars (zelena susceptible and jaguar partially resistant) were analyzed using a two-dimensional gel electrophoresis-based proteomic approach. initial symptoms on leaves (clearing v ...201525972878
infection of host plants by cucumber mosaic virus increases the susceptibility of myzus persicae aphids to the parasitoid aphidius colemani.plant viruses can profoundly alter the phenotypes of their host plants, with potentially far-reaching implications for ecology. yet few studies have explored the indirect, host-mediated, effects of plant viruses on non-vector insects. we examined how infection of cucurbita pepo plants by cucumber mosaic virus (cmv) impacted the susceptibility of aphids (myzus persicae) to attack by the parasitoid wasp aphidius colemani. in semi-natural foraging assays, we observed higher rates of aphid parasitis ...201526043237
draft genome sequence of erwinia tracheiphila, an economically important bacterial pathogen of cucurbits.erwinia tracheiphila is one of the most economically important pathogens of cucumbers, melons, squashes, pumpkins, and gourds in the northeastern and midwestern united states, yet its molecular pathology remains uninvestigated. here, we report the first draft genome sequence of an e. tracheiphila strain isolated from an infected wild gourd (cucurbita pepo subsp. texana) plant. the genome assembly consists of 7 contigs and includes a putative plasmid and at least 20 phage and prophage elements.201526044415
first report on fusarium wilt of zucchini caused by fusarium oxysporum, in korea.fusarium wilt of zucchini in jeonju, korea, was first noticed in may 2013. symptoms included wilting of the foliage, drying and withering of older leaves, and stunting of plants. infected plants eventually died during growth. based on morphological characteristics and phylogenetic analyses of the molecular markers (internal transcribed spacer rdna and translation elongation factor 1α), the fungus was identified as fusarium oxysporum. pathogenicity of a representative isolate was demonstrated via ...201526190927
suitability of zucchini and cucumber genotypes to populations of meloidogyne arenaria, m. incognita, and m. javanica.the host suitability of five zucchini and three cucumber genotypes to meloidogyne incognita (mipm26) and m. javanica (mj05) was determined in pot experiments in a greenhouse. the number of egg masses (em) did not differ among the genotypes of zucchini or cucumber, but the eggs/plant and reproduction factor (rf) did slightly. m. incognita mipm26 showed lower em, eggs/plant, and rf than m. javanica mj05. examination of the zucchini galls for nematode postinfection development revealed unsuitable c ...201525861120
[influence of a variation potential on photosynthesis in pumpkin seedlings (cucurbita pepo l.)].the influence of a variation potential on photosynthesis in pumpkin seedlings (cucurbita pepo l.) was investigated in our work. it was shown that the variation potential induced by cotyledon burning propagates into a leaf. it decreases co2 assimilation and transpiration as well as increases nonphotochemical quenching. investigation of isolated chloroplasts showed that lowering of the ph in incubation medium from 6.9-7.2 to 6.5 increases nonphotochemical quenching. it was proposed that lowering o ...201524159815
sphingomonas taxi, isolated from cucurbita pepo, proves to be a dde-degrading and plant growth-promoting strain.the draft genome of sphingomonas taxi, a strain of the sphingomonadaceae isolated from cucurbita pepo root tissue, is presented. this gram-negative bacterium shows 2,2-bis(p-chlorophenyl)-1,1-dichloroethylene (dde)-degrading potential and plant growth-promoting capacities. an analysis of its 3.9-mb draft genome will enhance the understanding of dde-degradation pathways and phytoremediation applications for dde-contaminated soils.201525977415
evaluation of the potential of squash pumpkin by-products (seeds and shell) as sources of antioxidant and bioactive compounds.the transformation of byproducts and wastes generated by agro-food companies is of high importance since only a small portion of plant material is utilized directly for human consumption. squash pumpkin is greatly used in portugal and as by-products of its processing are generated tons of shell and seeds. in this study we aim to evaluate the potential of these wastes as sources of beneficial and bioactive compounds (antioxidants and antimicrobials), studying the effect of different extraction so ...201525694712
an acorn squash (cucurbita pepo ssp. ovifera) fruit and seed transcriptome as a resource for the study of fruit traits in cucurbita.acorn squash (cucurbita pepo) is an iconic fall vegetable in the united states, known for its unique fruit shape and also prized for its culinary properties. little is known about the metabolism that underlies the development of fruit quality attributes such as color, sweetness, texture and nutritional qualities in acorn squash, or any other winter squash grown worldwide. to provide insight into winter squash fruit and seed development and add to the genomic resources in the cucurbita genus, rna ...201526504561
expression profile of carotenoid cleavage dioxygenase genes in summer squash (cucurbita pepo l.).carotenoids are important dietary components that can be found in vegetable crops. the accumulation of these compounds in fruit and vegetables is altered by the activity of carotenoid cleavage dioxygenases (ccds) enzymes that produce their degradation. the aim of this work was to study the possible implication of ccd genes in preventing carotenoid storage in the horticultural crop summer squash (cucurbita pepo l.). the relationship between the presence of these compounds and gene expression for ...201525861766
solution properties of a heteropolysaccharide extracted from pumpkin (cucurbita pepo, lady godiva).a water-soluble galactoglucofucomannan was extracted from pumpkin (cucurbita pepo, lady godiva variety). gc-ms analysis indicated that the polysaccharide was composed of 1,6-linked-glucosyl, 1,2,6-linked-mannosyl, 1,3,6-linked-mannosyl, 1,2,6-linked-galactosyl, 1,2,6-linked-galactosyl, terminal fucosyl and terminal glucose. the solution properties of the polysaccharide were studied systematically by using size-exclusion chromatography combined with multi-angle laser light scattering, viscometry ...201526256344
effect of phosphorylation on antioxidant activities of pumpkin (cucurbita pepo, lady godiva) polysaccharide.phosphorylated derivatives of pumpkin polysaccharide with different degree of substitution were synthesized using pocl3 and pyridine. antioxidant activities and cytoprotective effects of unmodified polysaccharide and phosphorylated derivatives were investigated employing various in vitro systems. results showed that high ratio of pocl3/pyridine could increase the degree of substitution and no remarkable degradation occurred in the phosphorylation process. characteristic absorption of phosphoryla ...201526231331
carbohydrate metabolism before and after dehiscence in the recalcitrant pollen of pumpkin (cucurbita pepo l.).pumpkin (cucurbita pepo l.) pollen is starchy, sucrose-poor and recalcitrant, features opposite to those of several model species; therefore, some differences in carbohydrate metabolism could be expected in this species. by studying pumpkin recalcitrant pollen, the objective was to provide new biochemical evidence to improve understanding of how carbohydrate metabolism might be involved in pollen functioning in advanced stages. four stages were analysed: immature pollen from 1 day before anthesi ...201525353653
uptake of pharmaceuticals influences plant development and affects nutrient and hormone homeostases.the detection of a range of active pharmaceutical ingredients (apis) in the soil environment has led to a number of publications demonstrating uptake by crops, however very few studies have explored the potential for impacts on plant development as a result of api uptake. this study investigated the effect of carbamazepine and verapamil (0.005-10 mg/kg) on a range of plant responses in zucchini (cucurbita pepo). uptake increased in a dose-dependent manner, with maximum leaf concentrations of 821 ...201526418514
investigating differences in the root to shoot transfer and xylem sap solubility of organic compounds between zucchini, squash and soybean using a pressure chamber method.a pressure chamber method was used to examine differences in the root to shoot transfer and xylem sap solubility of caffeine (log kow=-0.07), triclocarban (log kow=3.5-4.2) and endosulfan (log kow=3.8-4.8) for zucchini (cucurbita pepo ssp pepo), squash (cucurbita pepo ssp ovifera), and soybean (glycine max l.). transpiration stream concentration factors (tscf) for caffeine (tscf=0.8) were statistically equivalent for all plant species. however, for the more hydrophobic endosulfan and triclocarba ...201525537866
anticancer and anti-inflammatory activities of some dietary cucurbits.in this study, we investigated few dietary cucurbits for anticancer activity by monitoring cytotoxic (mtt and ldh assays), apoptotic (caspase-3 and annexin-v assays), and also their anti-inflammatory effects by il-8 cytokine assay. aqua-alcoholic (50:50) whole extracts of cucurbits [lagenaria siceraria (ls), luffa cylindrica (lc) and cucurbita pepo (cp)] were evaluated in colon cancer cells (ht-29 and hct-15) and were compared with isolated biomolecule, cucurbitacin-b (cbit-b). mtt and ldh assay ...201526011982
two new ent-kaurane-type diterpene glycosides from zucchini (cucurbita pepo l.) seeds.two new ent-kaurane diterpene glycosides; 12α-(β-d-glucopyranosyloxy)-7β-hydroxykaurenolide (1) and 7β-(β-d-glucopyranosyloxy)-12α-hydroxykaurenolide (2), a new steroid; (24s)-stigmasta-7,22e,25-trien-3-one (12), and known compounds (3-11, 13-14) were isolated from zucchini (cucurbita pepo l.) seeds. the absolute structures of 1 and 2 were determined by acid hydrolysis and application of a modified moscher's method. furthermore, isolated compounds (1-14), and a derivative, 1a, were evaluated for ...201526420344
degradation of aromatic compounds in plants grown under aseptic conditions.the aim of the work is to investigate the ability of higher plants to absorb and detoxify environmental pollutants - aromatic compounds via aromatic ring cleavage. transformation of 14c specifically labelled benzene derivatives, [1-6-14c]-nitrobenzene, [1-6-(14)c]-aniline, [1-(14)c]- and [7-(14)c]-benzoic acid, in axenic seedlings of maize (zea mays l.), kidney bean (phaseolus vulgaris l.), pea (pisum sativum l.) and pumpkin (cucurbita pepo l.) were studied. after penetration in plants, the abov ...201515787252
hormonal and epigenetic regulation during embryogenic tissue habituation in cucurbita pepo l.habituated embryogenic line of pumpkin contained more cks and iaa, but less aba than the non-habituated line. pronounced hypomethylation correlated with the absence of 2,4-d, addition of 5-azac, and the process of habituation. a comparative analysis between habituated and non-habituated embryogenic cultures of pumpkin (cucurbita pepo l.) in relation to endogenous phytohormones, global dna methylation, and developmental and regeneration capacities of the cultures was conducted. the analysis revea ...201626403461
plant extract-mediated biogenic synthesis of silver, manganese dioxide, silver-doped manganese dioxide nanoparticles and their antibacterial activity against food- and water-borne pathogens.silver nanoparticles (agnps), manganese dioxide nanoparticles (mno₂nps) and silver-doped manganese dioxide nanoparticles (ag-doped mno₂nps) were synthesized by simultaneous green chemistry reduction approach. aqueous extract from the leaves of medicinally important plant cucurbita pepo was used as reducing and capping agents. various characterization techniques were carried out to affirm the formation of nanoparticles. hr-tem analysis confirmed the size of nanoparticles in the range of 15-70 nm ...201626857369
characterization of light-dependent regulation of state transitions in gymnosperms.the goal of this study was to characterize the light-dependent regulation of state transitions in gymnosperms. two species of conifer were examined: eastern white pine (pinus strobus l.) and white spruce [picea glauca (moench) voss], as well as the angiosperm pumpkin (cucurbita pepo l. subsp. pepo). both diurnal time courses in the field and manipulated light experiments in growth chambers were conducted. results from chlorophyll fluorescence analysis indicated that pumpkin was able to use a lar ...201626802541
bioavailability assessments following biochar and activated carbon amendment in ddt-contaminated soil.the effects of 2.8% w/w granulated activated carbon (gac) and two types of biochar (burt's and blueleaf) on ddt bioavailability in soil (39 μg/g) were investigated using invertebrates (eisenia fetida), plants (cucurbita pepo spp. pepo) and a polyoxymethylene (pom) passive sampler method. biochar significantly reduced ddt accumulation in e. fetida (49%) and showed no detrimental effects to invertebrate health. in contrast, addition of gac caused significant toxic effects (invertebrate avoidance a ...201626495827
exposure of cucurbita pepo to dde-contamination alters the endophytic community: a cultivation dependent vs a cultivation independent approach.2,2-bis(p-chlorophenyl)-1,1-dichloro-ethylene (dde) is the most abundant and persistent degradation product of the pesticide 2,2-bis(p-chlorophenyl)-1,1,1-trichloroethane (ddt) and is encountered in contaminated soils worldwide. both dde and ddt are classified as persistent organic pollutants (pops) due to their high hydrophobicity and potential for bioaccumulation and biomagnification in the food chain. zucchini (cucurbita pepo ssp. pepo) has been shown to accumulate high concentrations of dde ...201626683261
comparison between cultivated and total bacterial communities associated with cucurbita pepo using cultivation-dependent techniques and 454 pyrosequencing.endophytic bacteria often have beneficial effects on their host plants that can be exploited for bioremediation applications but, according to the literature, only 0.001-1% of all endophytic microbes should be cultivable. this study compared the cultivated endophytic communities of the roots and shoots of cucurbita pepo with the total endophytic communities as determined by cultivation-dependent techniques and 454 pyrosequencing. the ten most abundant taxa of the total communities aligned well w ...201626656884
the evaluation of the healing proprieties of pumpkin and linseed oils on deep second-degree burns in rats.medicinal plants have been recognized as useful remedies for primary health care. accordingly, cucurbita pepo l. (cucurbitaceae) (pumpkin) and linum usitatissimum (l.) griesb. (linaceae) (linseed) which have extracted oil with prominent pharmacological properties are investigated as possible burn healing treatments.201626186459
effect of oil extraction assisted by ultrasound on the physicochemical properties and fatty acid profile of pumpkin seed oil (cucurbita pepo).the effects of amplitude and time of ultrasound-assisted extraction on the physicochemical properties and the fatty acid profile of pumpkin seed oil (cucurbita pepo) were evaluated. ultrasound time (5-30 min) and the response variables amplitude (25-100%), extraction yield, efficiency, oxidative stability in terms of the free fatty acids (ffa) of the plant design comprising two independent experiments variables, peroxide (pv), p-anisidine (av), totox value (tv) and the fatty acid profile were ev ...201626964969
plant growth regulators induced urease activity in cucurbita pepo l. cotyledons.this study is aimed to investigate the activity of urease (ec 3.5.1.5, urea amidohydrolase) that catalyzes the hydrolysis of urea in 5-day-old cucurbita pepo cotyledons subjected to various concentrations of different growth regulators. the treatment of c. pepo cotyledons with different concentrations (100-600 μmol) of different auxins [indole-3-acetic acid (iaa), indole butyric acid (iba), indole propionic acid (ipa) and naphthalene acetic acid (naa)]; or with different concentrations (100-300 ...201626960356
health hazards and heavy metals accumulation by summer squash (cucurbita pepo l.) cultivated in contaminated soils.the present study was carried out to investigate the heavy metal concentration accumulated by summer squash cultivated in contaminated soil and their health hazards for public consumers at south cairo province, egypt. soil and plants were sampled from contaminated and reference farms, using 1 m(2) quadrats, for biomass estimation and nutrient analysis. the daily intake of metals (dim) and health risk index (hri) were estimated. significant differences in soil variables (except as) between contam ...201627344559
italian horticultural and culinary records of summer squash (cucurbita pepo, cucurbitaceae) and emergence of the zucchini in 19th-century milan.summer squash, the young fruits of cucurbita pepo, are a common, high-value fruit vegetable. of the summer squash, the zucchini, c. pepo subsp. pepo zucchini group, is by far the most cosmopolitan. the zucchini is easily distinguished from other summer squash by its uniformly cylindrical shape and intense colour. the zucchini is a relatively new cultivar-group of c. pepo, the earliest known evidence for its existence having been a description in a book on horticulture published in milan in 1901. ...201627343231
oil from pumpkin (cucurbita pepo l.) seeds: evaluation of its functional properties on wound healing in rats.increasing natural drug demand for pharmaceutical uses has encouraged scientifics all over the world to explore medicinal plants recognized as efficient remedies. in this context, extracted oil from pumpkin seeds (cucurbita pepo l.) is an interesting target, as it is composed with prominent pharmacological properties to possible wound healing treatments.201627068642
arbuscular mycorrhizal fungal hyphae alter soil bacterial community and enhance polychlorinated biphenyls dissipation.we investigated the role of arbuscular mycorrhizal fungal (amf) hyphae in alternation of soil microbial community and aroclor 1242 dissipation. a two-compartment rhizobox system with double nylon meshes in the central was employed to exclude the influence of cucurbita pepo l. root exudates on hyphal compartment soil. to assess the quantitative effect of amf hyphae on soil microbial community, we separated the hyphal compartment soil into four horizontal layers from the central mesh to outer wall ...201627379068
genome sequence analysis of two south african isolates of moroccan watermelon mosaic virus infecting cucurbits.moroccan watermelon mosaic virus (mwmv) has been prevalent in cucurbits in the republic of south africa (rsa) since it was first reported in 1987. however, full genome studies of the south african isolates have never been conducted previously. the full genome of two mwmv isolates infecting cucurbits (cucurbita pepo l.) in the province of kwazulu-natal, rsa, was compared with the genome of the tunisian isolate in this communication. the genome sequences of the rsa mwmv isolates were elucidated us ...201627448681
multicolor fluorescence imaging as a candidate for disease detection in plant phenotyping.the negative impact of conventional farming on environment and human health make improvements on farming management mandatory. imaging techniques are implemented in remote sensing for monitoring crop fields and plant phenotyping programs. the increasingly large size and complexity of the data obtained by these techniques, makes the implementation of powerful mathematical tools necessary in order to identify informative parameters and to apply them in precision agriculture. multicolor fluorescenc ...201627994607
zucchini shoestring virus: a distinct potyvirus in the papaya ringspot virus cluster.zucchini shoestring virus (zssv) has been proposed to be a putative potyvirus in the papaya ringspot virus (prsv) cluster, based on the sequence similarity of its coat protein to those of related potyviruses. zssv has been associated with the outbreak of a damaging disease of baby marrow (cucurbita pepo l.) that had been observed throughout the province of kwazulu-natal, in the republic of south africa (rsa). we report the genome sequence of zssv, determined by next-generation sequencing of tota ...201627216927
effects of virus infection on pollen production and pollen performance: implications for the spread of resistance alleles.studies over the past 25 years have shown that environmental stresses adversely affect male function, including pollen production and pollen performance (germination and pollen tube growth rate). consequently, genetic variation among plants in resistance to a stress has the potential to impact pollen donation to conspecifics and, if deposited onto a stigma, the ability of the pollen to achieve fertilization. we examined the effects of a nonlethal virus epidemic on pollen production and pollen pe ...201626905087
electrophysiology of pumpkin seeds: memristors in vivo.leon chua, the discoverer of a memristor, theoretically predicted that voltage gated ion channels can be memristors. we recently found memristors in different plants such as the venus flytrap, mimosa pudica, aloe vera, apple fruits, and in potato tubers. there are no publications in literature about the existence of memristors in seeds. the goal of this work was to discover if pumpkin seeds might have memristors. we selected cucurbita pepo l., cv. cinderella, cucurbita maxima l. cv warty goblin, ...201626926652
curcurbita pepo subspecies delineates striped cucumber beetle (acalymma vittatum) preference.the striped cucumber beetle (acalymma vittatum (f.)) is a destructive pest of cucurbit crops, and management could be improved by host plant resistance, especially in organic farming systems. however, despite the variation in striped cucumber beetle preference observed within the economically important species, cucurbita pepo l., plant breeders and entomologists lacked a simple framework to classify and exploit these differences. this study used recent phylogenetic evidence and bioassays to orga ...201627347423
preference of bemisia tabaci biotype b on zucchini squash and buckwheat and the effect of delphastus catalinae on whitefly populations.zucchini squash, cucurbita pepo l., is an important vegetable crop in florida. physiological disorders and insect-transmitted diseases are major problems for squash growers in semi-tropical regions around the world. bemisia tabaci (gennadius) biotype b is a significant whitefly pest and is largely responsible for transmitting viruses and causing physiological disorders in squash. several studies have shown that whitefly populations are reduced when crops are interplanted with non-host cover crop ...201626388111
de novo transcriptome assembly of two contrasting pumpkin cultivars.cucurbita pepo (squash, pumpkin, gourd), a worldwide-cultivated vegetable of american origin, is extremely variable in fruit characteristics. however, the information associated with genes and genetic markers for pumpkin is very limited. in order to identify new genes and to develop genetic markers, we performed a transcriptome analysis (rna-seq) of two contrasting pumpkin cultivars. leaves and female flowers of cultivars, 'big moose' with large round fruits and 'munchkin' with small round fruit ...201626981408
molecular response of crop plants to engineered nanomaterials.functional toxicology has enabled the identification of genes involved in conferring tolerance and sensitivity to engineered nanomaterial (enm) exposure in the model plant arabidopsis thaliana (l.) heynh. several genes were found to be involved in metabolic functions, stress response, transport, protein synthesis, and dna repair. consequently, analysis of physiological parameters, metal content (through icp-ms quantification), and gene expression (by rt-qpcr) of a. thaliana orthologue genes were ...201627301997
the potential influence of bumble bee visitation on foraging behaviors and assemblages of honey bees on squash flowers in highland agricultural ecosystems.bee species interactions can benefit plant pollination through synergistic effects and complementary effects, or can be of detriment to plant pollination through competition effects by reducing visitation by effective pollinators. since specific bee interactions influence the foraging performance of bees on flowers, they also act as drivers to regulate the assemblage of flower visitors. we selected squash (cucurbita pepo l.) and its pollinators as a model system to study the foraging response of ...201626765140
efficacy of various application methods of fluensulfone for managing root-knot nematodes in vegetables.fluensulfone is a new nematicide in the flouroalkenyl chemical group. a field experiment was conducted in 2012 and 2013 to evaluate the efficacy of various application methods of fluensulfone for control of meloidogyne spp. in cucumber (cucumis sativus). treatments of fluensulfone (3.0 kg a.i./ha) were applied either as preplant incorporation (ppi) or via different drip irrigation methods: drip without pulse irrigation (drip np), pulse irrigation 1 hr after treatment (drip +1p), and treatment at ...201627418698
companion planting with white yarrow or with feverfew for squash bug, anasa tristis (hemiptera: coreidae), management on summer squash.the squash bug, anasa tristis (de geer), is a major insect pest of cucurbits. control of squash bugs with insecticidal chemicals is difficult to achieve. we investigated the potential of companion planting with white yarrow (achillea millefolium l.) or feverfew [tanacetum parthenium (l.) sch. bip.] for squash bug management in field plantings of summer squash (cucurbita pepo l.).201627604891
pumpkin seed extract: cell growth inhibition of hyperplastic and cancer cells, independent of steroid hormone receptors.pumpkin seeds have been known in folk medicine as remedy for kidney, bladder and prostate disorders since centuries. nevertheless, pumpkin research provides insufficient data to back up traditional beliefs of ethnomedical practice. the bioactivity of a hydro-ethanolic extract of pumpkin seeds from the styrian pumpkin, cucurbita pepo l. subsp. pepo var. styriaca, was investigated. as pumpkin seed extracts are standardized to cucurbitin, this compound was also tested. transactivational activity wa ...201626976217
establishment of a simple and rapid gene delivery system for cucurbits by using engineered of zucchini yellow mosaic virus.the infectious full-length cdna clone of zucchini yellow mosaic virus (zymv) isolate pa (pzymv-pa), which was isolated from pumpkin, was constructed by utilizing viral transcription and processing signals to produce infectious in vivo transcripts. simple rub-inoculation of plasmid dnas of pzymv-pa was successful to cause infection of zucchini plants (cucurbita pepo l.). we further engineered this infectious cdna clone of zymv as a viral vector for systemic expression of heterologous proteins in ...201626889118
optimum concentrations of trichoderma longibrachiatum and cadusafos for controlling meloidogyne javanica on zucchini plants.a factorial experiment was established in a completely randomized design to verify the effect of different inoculum levels of an iranian isolate of trichoderma longibrachiatum separately and in combination with various concentrations of cadusafos against meloidogyne javanica in the greenhouse. zucchini seeds were soaked for 12 hr in five densities (0, 10(5), 10(6), 10(7), and 10(8) spores/ml suspension) of the fungus prior to planting in pots containing four concentrations of cadusafos (0, 0.5, ...201627168653
the role of cucurbita pepo in the management of patients affected by lower urinary tract symptoms due to benign prostatic hyperplasia: a narrative review.phytotherapeutic compounds are largely used in the treatment of lower urinary tract symptoms (luts) related to benign prostatic hyperplasia (bph) due to low side-effect profiles and costs, high level of acceptance by patients and a low rate of dropout. here, we aimed to analyze all available evidence on the role of cucurbita pepo in the treatment of luts-bph.201627377091
application of cavitation system to accelerate aqueous enzymatic extraction of seed oil from cucurbita pepo l. and evaluation of hypoglycemic effect.cavitation-accelerated aqueous enzymatic extraction (caee) of seed oil from cucurbita pepo was performed. an enzyme cocktail comprised of cellulose, pectinase and proteinase can work synergistically in releasing the oil. the caee extraction conditions were optimized by a plackett-burman design followed by a central composite methodology. a maximal extraction yield of 58.06% was achieved under optimal conditions of vacuum degree -0.07, enzyme amount 1.05% and extraction time 69min. as compared to ...201627374549
potential for phytoremediation of pcdd/pcdf-contaminated sludge and sediments using cucurbitaceae plants: a pilot study.the current study evaluates the impact of sewage sludge and urban reservoir sediment on changes in total and toxic equivalency (teq) pcdd/pcdf concentration in soil and phytotoxicity measured using three test species: lepidium sativum, sinapis alba, and sorghum saccharatum, during 5 weeks of cucurbita pepo l. cv 'atena polka' (zucchini) cultivation. 'atena polka' was found to reduce total pcdd/pcdf concentration by 37 % in soil amended with sludge and 32 % in soil treated with sediment from an u ...201627365136
radioprotective potential of lagenaria siceraria extract against radiation-induced gastrointestinal injury.the cucurbits (prebiotics) were investigated as novel agents for radio-modification against gastrointestinal injury. the cell-cycle fractions and dna damage were monitored in hct-15 cells. a cucurbit extract was added to culture medium 2 h before irradiation (6 gy) and was substituted by fresh medium at 4 h post-irradiation. the whole extract of the fruits of lagenaria siceraria, luffa cylindrica, or cucurbita pepo extract enhanced g2 fractions (42%, 34%, and 37%, respectively) as compared with ...201627863208
comparative analysis of cucurbita pepo metabolism throughout fruit development in acorn squash and oilseed pumpkin.both the fruit mesocarp and the seeds of winter squash can be used for consumption, although the focus of breeding efforts varies by cultivar. cultivars bred for fruit consumption are selected for fruit mesocarp quality traits such as carotenoid content, percent dry matter, and percent soluble solids, while these traits are essentially ignored in oilseed pumpkins. to compare fruit development in these two types of squash, we sequenced the fruit transcriptome of two cultivars bred for different p ...201627688889
evaluation of anthelmintic activity and composition of pumpkin (cucurbita pepo l.) seed extracts-in vitro and in vivo studies.a significant number of studies report growing resistance in nematodes thriving in both humans and livestock. this study was conducted to evaluate the in vitro and in vivo anthelmintic efficiency of curcubita pepo (c. pepo) l. hot water extract (hwe), cold water extract (cwe) or ethanol extract (ete) on two model nematodes: caenorhabditis elegans (c. elegans) and heligmosoides bakeri (h. bakeri).201627598135
colonic perforation in a young tetraplegic male caused by zucchini.colonic perforation is a clinical condition which occurs due to variety of reasons, such as intrinsic disorders of the intestine, extrinsic causes, but also due to presence of foreign bodies. foreign objects enter gastrointestinal tract by oral or transanal introduction.201627994305
cultivar-based introgression mapping reveals wild species-derived pm-0, the major powdery mildew resistance locus in squash.powdery mildew is a major fungal disease on squash and pumpkin (cucurbita spp.) in the us and throughout the world. genetic resistance to the disease is not known to occur naturally within cucurbita pepo and only infrequently in cucurbita moschata, but has been achieved in both species through the introgression of a major resistance gene from the wild species cucurbita okeechobeensis subsp. martinezii. at present, this gene, pm-0, is used extensively in breeding, and is found in nearly all powde ...201627936008
involvement of α-, γ- and δ-tocopherol isomers from
pumpkin (cucurbita pepo l.) seed oil or oil mixtures in
the biphasic dpph˙ disappearance kinetics.the antioxidant activity of three types of pumpkin seed oil or oil mixtures (cold- -pressed, produced from roasted seed paste and salad) produced in the northern part of croatia and the kinetics of their behaviour as free radical scavengers were investigated using dpph˙. in addition, the involvement of oil tocopherol isomers (α-, γ- and δ-) in different steps of dpph˙ disappearance and their impact on the rate of reaction were analysed. the kinetics of dpph˙ disappearance is a two-step process. ...201627904410
oviposition preferences of pickleworm (lepidoptera: crambidae) in relation to a potential push-pull cropping management approach.pickleworm, diaphania nitidalis cramer (lepidoptera: crambidae), is a major pest of cucurbits. the current management approach for this pest is weekly insecticide applications. a push-pull cropping approach may be an alternative management practice and could reduce reliance on pesticides. one potential push-pull scenario is the use of squash (cucurbita pepo l.) as a trap crop and watermelon (citrullus lanatus [thunb.] matsum. & nakai) as a deterrent intercrop to manage pickleworm on cantaloupe ( ...201627118111
novel edible oil sources: microwave heating and chemical properties.the aim of this work was to investigate the effect of various microwave heating times (1, 3, 5, 10, and 15min) on the chemical properties of novel edible oil sources, including mashhadi melon (cucumis melo var. iranians cv. mashhadi), iranian watermelon (citrullus lanatus cv. fire fon), pumpkin (cucurbita pepo subsp. pepo var. styriaca), and yellow apple (malus domestica cv. golden delicious) seed oils. the evaluated parameters were peroxide value (pv), conjugated diene (cd) and triene (ct) valu ...201728290292
antioxidative activities and phenolic compounds of pumpkin (cucurbita pepo) seeds and amaranth (amaranthus caudatus) grain extracts.phenolic compounds were extracted from pumpkin (cucurbita pepo) seed and amaranth (amaranthus caudatus) grain into 80% (v/v) methanol. the extracts obtained were characterised by the contents of total phenolic compounds (tpc), trolox equivalent antioxidant capacity (teac), ferric-reducing antioxidant power (frap) and antiradical activity against 2,2-diphenyl-1-picrylhydrazyl (dpph(·)) radical. the content of individual phenolic compounds was determined by hplc-dad method. pumpkin seeds showed th ...201728114838
an snp-based saturated genetic map and qtl analysis of fruit-related traits in zucchini using genotyping-by-sequencing.cucurbita pepo is a cucurbit with growing economic importance worldwide. zucchini morphotype is the most important within this highly variable species. recently, transcriptome and simple sequence repeat (ssr)- and single nucleotide polymorphism (snp)-based medium density maps have been reported, however further genomic tools are needed for efficient molecular breeding in the species. our objective is to combine currently available complete transcriptomes and the zucchini genome sequence with hig ...201728100189
agroindustrial compost as a peat alternative in the horticultural industry of ecuador.this work was conducted in order to investigate the possibility of using different agroindustrial composts in the production of horticultural seedlings, thereby replacing part of the peat in the growing media. three vegetable species differing in salt sensitivity - tomato (solanum lycopersicum l. var. malpica) (the least sensitive), courgette (cucurbita pepo l. var. mastil f1) (moderately sensitive) and pepper (capsicum annuum l. var. largo de reus pairal) (the most sensitive) - were grown in ni ...201727815007
pepo aphid-borne yellows virus: a new species in the genus polerovirus.pepo aphid-borne yellows virus (pabyv) has been proposed as a putative representative of a new species in the genus polerovirus in the family luteoviridae. the genomes of two south african (sa) isolates of cucurbit-infecting pabyv were described in this record. total rna, extracted from a pattypan (cucurbita pepo l.) and a baby marrow (c. pepo l.) leaf samples, was subjected to next-generation sequencing (ngs) on the hiseq illumina platform. sanger sequencing was subsequently used to authenticat ...201727623838
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