effects of amendments on the uptake and distribution of ddt in cucurbita pepo ssp pepo plants.the effects of soil amendments on the phytoextraction of summation operatorddt (ddt + ddd + dde) from soil ([ summation operatorddt] approximately 1500 ng/g) by a pumpkin variety of cucurbita pepo ssp pepo were tested and the patterns of summation operatorddt storage throughout the plant shoot were examined. the soil amendments did not increase the total amount of summation operatorddt extracted into plant shoots, but new information about summation operatorddt distribution in the plants was obt ...201019762136
the effects of planting methods and head pruning on seed yield and yield components of medicinal pumpkin (cucurbita pepo subsp. pepo convar. pepo var. styriaca) at low temperature areas.this experiment carried out to evaluate the effects of planting methods (seed sowing and transplanting) and head pruning (no pruning, pruning after 12th node and pruning after 16th node) on yield and yield components such as number of branches (sub-branches) per plant, fruits per plant, growth, fruit size, weight of fresh fruit, weight of seeds per fruit, number of seeds per fruit and seed yield of medicinal pumpkin. the experiment was carried out based of factorial experiment with randomized co ...200919580007
enhancing the release and plant uptake of pahs with a water-soluble purine alkaloid.the effect of a common plant alkaloid, caffeine, on the release and plant uptake of some polycyclic aromatic hydrocarbons (pahs) in soils was investigated. cucurbita pepo (ssp. pepo cv. gold rush) was grown in pah-spiked media in the presence and absence of caffeine. solubility tests initially confirmed the ability of caffeine to dissolve pahs mixtures of anthracene, phenanthrene, pyrene, benzo[a]pyrene and benzo[ghi]perylene. extraction experiments also highlighted its potential as a pah-releas ...200919477480
cadmium induced changes in subcellular glutathione contents within glandular trichomes of cucurbita pepo l.plants cope with cadmium (cd) stress by complexation with phytochelatins (pc), metallothioneins and glutathione and sequestration within vacuoles. especially glutathione was found to play a major role in cd detoxification as cd shows a high binding affinity towards thiols and as glutathione is a precursor for pc synthesis. in the present study, we have used an immunohistochemical approach combined with computer-supported transmission electron microscopy in order to measure changes in the subcell ...201019424775
leaf herbivory increases floral fragrance in male but not female cucurbita pepo subsp. texana (cucurbitaceae) flowers.mutualisms are key interactions that affect population dynamics and structure communities, but the extent to which mutualists can attract potential partners may depend on community context. many studies have shown that leaf herbivory reduces pollinator visitation and have focused on reduced floral visual display and rewards as potential mechanisms. however, olfactory display plays a critical role in mediating interactions between plants, herbivores, and pollinators. we simulated leaf damage in c ...200921628242
influence of cover crop and intercrop systems on bemisia argentifolli (hemiptera: aleyrodidae) infestation and associated squash silverleaf disorder in zucchini.field experiments were conducted to evaluate the effects of cover cropping and intercropping on population densities of silverleaf whitefly, bemisia argentifolli bellow and perring, and the incidence of squash silverleaf disorder (ssl) in zucchini, cucurbita pepo l., in oahu, hi. two cover crops, buckwheat (bw), fagopyrum esculentum moench, and white clover (wc), trifolium repens l., or sunn hemp (sh), crotolaria juncea l., and an intercropped vegetable, okra, abelmonchus esculentus l., were eva ...200919389294
nanoparticle penetration and transport in living pumpkin plants: in situ subcellular recent years, the application of nanotechnology in several fields of bioscience and biomedicine has been studied. the use of nanoparticles for the targeted delivery of substances has been given special attention and is of particular interest in the treatment of plant diseases. in this work both the penetration and the movement of iron-carbon nanoparticles in plant cells have been analyzed in living plants of cucurbita pepo.200919389253
initial formation of an indigenous crop complex in eastern north america at 3800 b.p.although geneticists and archaeologists continue to make progress world-wide in documenting the time and place of the initial domestication of a growing number of plants and animals, far less is known regarding the critically important context of coalescence of various species into distinctive sets or complexes of domesticates in each of the world's 10 or more independent centers of agricultural origin. in this article, the initial emergence of a crop complex is described for one of the best-doc ...200919366669
ultraweak and induced photon emission after wounding of plants.the ultraweak and induced photon emission were measured by a single photon counting equipment (photomultiplier hamamatsu r562) on cucurbita pepo variaca styriacae after wounding. wounding significantly changes the emission from a stationary to a nonstationary state and the shape of the decay curve obtained after light illumination. the rise in the ultraweak photon emission depends on the kind of wounding and its localization on the plant. the decay curves obtained after wounding could be better ...200919254235
monitoring diabrotica v. virgifera (col.: chrysomelidae) in southeastern slovenia: increasing population trend and host spectrum expansion.ever since the western corn rootworm (wcr) (diabrotica virgifera virgifera), an alien invasive species from north america, has been introduced into europe on at least 3 separate occasions, it spread within 15 years over the entire area of south-eastern and central europe (except denmark). until quite recently, zea mays l. was the only known host plant whereas in north america wcr also attacks members of the plant family cucurbitaceae. in august of 2006, we were able to validate these findings al ...200819226789
plant mitochondria electron partitioning is independent of short-term temperature changes.we tested the hypotheses that relative activity of the less efficient alternative oxidase (aox) path changes with diurnal temperature changes, and thus changes carbon use efficiency with temperature. the activities of the alternative and cytochrome oxidase (cox) paths in plant tissues of three species were determined by measuring 18o/16o discrimination and total respiration from 17 to 36 degrees c. a new, more accurate method for calculating oxygen uptake rate from the mass spectrometry data was ...200919210639
genes for "reverse" fruit striping in squash (cucurbita pepo).longitudinal striping is a common fruit color pattern in cucurbita pepo and is conferred by any of several alleles at the light coloration-1 locus. normally, dark stripes appear over the fruit surface areas between the 10 main subepidermal vein tracts with the lighter background color over and adjacent to these vein tracts. less commonly, the striping is "reversed," that is, lighter than the background color. the present work was conducted to elucidate the mode of inheritance of reverse striping ...200919153086
synergic effect studies of the bi-enzymatic system laccase-peroxidase in a voltammetric biosensor for catecholamines.several bi-enzymatic carbon paste biosensors modified with enzymes laccase from pleurotus ostreatus fungi and peroxidase from zucchini (cucurbita pepo) were constructed for evaluating the synergic effect of the two enzymes on the voltammetric biosensor response for various catecholamines. initially was investigated the effect of ph from 5.0 to 7.5, temperature from 25 to 50 degrees c, initial stirring time from 30 to 150 s, scan rate from 10 to 60 mvs(-1) and potential pulse amplitude from 10 to ...200318968977
differential uptake for dioxin-like compounds by zucchini subspecies.the zucchini (cucurbita pepo) cultivars 'patty green', 'black beauty', and 'gold rush' were cultivated on weathered dioxin-contaminated soil in pots, and concentrations of the 29 dioxin-like compounds that were assigned who-tefs, three non-toxic polychlorinated dibenzo-p-dioxins/dibenzofurans (pcdd/fs), and two non-dioxin-like polychlorinated biphenyls (pcbs) were analyzed. toxic equivalent (teq) values accumulated in 'black beauty' and 'gold rush' were about 180 times higher than those in 'patt ...200818835616
senescence progression in a single darkened cotyledon depends on the light status of the other cotyledon in cucurbita pepo (zucchini) seedlings: potential involvement of cytokinins and cytokinin oxidase/dehydrogenase activity.darkness mediates different senescence-related responses depending on the targeting of dark treatment (whole plants or individual leaves) and on the organs that perceive the signal (leaves or cotyledons). as no data are available on the potential role of darkness to promote senescence when applied to individual cotyledons, we have investigated how darkness affects the progression of senescence in either a single or both individually darkened cotyledons of young 10-day-old cucurbita pepo (zucchin ...200818823328
the effects of repeated planting, planting density, and specific transfer pathways on pcb uptake by cucurbita pepo grown in field in situ field investigation into the potential of pcb phytoextraction by cucurbita pepo ssp. pepo (pumpkin) plants was continued for a second year at a field site known to be contaminated with a mixture of aroclors 1254 and 1260 (average soil [pcb]=21 microg/g). plant stem and leaf pcb concentrations in this second field season (11 and 8.9 microg/g, respectively) were observed to increase significantly from the stem and leaf pcb concentrations reported in the previous year (5.7 and 3.9 microg ...200818786697
differential effects of methyl jasmonate on growth and division of etiolated zucchini cotyledons.the jasmonates are well studied in the context of plant defence but increasingly are also recognised as playing roles in development. in many systems, jasmonates antagonise the effects of cytokinins. the aim of the present work was to elucidate interactions between methyl jasmonate and cytokinin (benzyladenine) in regulating growth of zucchini (cucurbita pepo l., cv. cocozelle, var. tripolis) cotyledons, taking advantage of the ability to simultaneously quantify cell enlargement and division fro ...200818557907
response of citrus peelminer marmara gulosa guillén and davis (lepidoptera: gracillariidae) stages to various insecticides.egg and larval stages of citrus peelminer marmara gulosa guillén and davis infesting zucchini squash cucurbita pepo l. var 'revenue' were exposed in the laboratory to a wide range of insecticide classes used in california citrus (organophosphate, carbamate, pyrethroid, neonicotinoid, insect growth regulator, fermentation products and sulfur) to determine peelminer response with and without a non-ionic adjuvant or oil.200818506674
ascorbic acid conjugates isolated from the phloem of cucurbitaceae.analysis of phloem exudates from the fruit of cucurbitaceae revealed the presence of several compounds with uv-visible absorption spectra identical to that of l-ascorbic acid. in cucurbita pepo l. (zucchini), the compounds could be isolated from phloem exudates collected from aerial parts of the plant but were not detected in whole tissue homogenates. the compounds isolated from the phloem exudates of c. pepo fruit were eluted from strong anion exchange resin in the same fraction as l-ascorbic a ...200818472116
diabrotica virgifera virgifera leconte: inconspicuous leaf beetle--formidable challenges to the universe of entomology with its close to one million described and an estimated ten million undescribed species, diabrotica virgifera virgifera leconte (coleoptera: chrysometidae), the western corn rootworm (wcr), is one of the economically most important pests. it causes annual treatment costs and damages of a billion dollars in the us (metcalf 1986). similar costs are predicted for europe which the beetle invaded 15 years ago. due to lack of natural enemies it is now expanding its terri ...200718399421
impact of daily consumption of iron fortified ready-to-eat cereal and pumpkin seed kernels (cucurbita pepo) on serum iron in adult women.iron deficiency, anemia, is the most prevalent nutritional problem in the world today. the objective of this study was to consider the effectiveness of consumption of iron fortified ready-to-eat cereal and pumpkin seed kernels as two sources of dietary iron on status of iron nutrition and response of hematological characteristics of women at reproductive ages. eight healthy female, single or non pregnant subjects, aged 20-37 y consumed 30 g of iron fortified ready-to-eat cereal (providing 7.1 mg ...200718198398
resistance and tolerance to herbivory changes with inbreeding and ontogeny in a wild gourd (cucurbitaceae).herbivory is a ubiquitous component of terrestrial communities that reduces plant growth and reproduction. consequently, a goal of evolutionary ecology is to identify the causes and consequences of variation in herbivory within plant populations. this three-year study examined the effects of inbreeding on the resistance of wild gourd plants (cucurbita pepo subsp. texana) to herbivory by cucumber beetles and the impact of the timing of herbivory on reproduction. we grew families of inbred and out ...200821632318
nanoparticles as smart treatment-delivery systems in plants: assessment of different techniques of microscopy for their visualization in plant tissues.the great potential of using nanodevices as delivery systems to specific targets in living organisms was first explored for medical uses. in plants, the same principles can be applied for a broad range of uses, in particular to tackle infections. nanoparticles tagged to agrochemicals or other substances could reduce the damage to other plant tissues and the amount of chemicals released into the environment. to explore the benefits of applying nanotechnology to agriculture, the first stage is to ...200817998213
the effect of stem pruning and nitrogen levels of on some physico-chemical characteristics of pumpkin seed (cucurbita pepo l.).to investigate the effects of stem pruning (no heading, head pruning of stem after formation of 10 and 14 nodes) and nitrogen levels (0, 50, 100 and 200 kg ha(-1)) on physical and chemical characteristic of pumpkin seed a factorial experiment based on randomized complete block design with three replication was carried out in gorgan at 2003 and repeated in 2004 years. results showed that the stem pruning has significant effect on traits such as seed oil, linoleic acid and oleic acid content. nitr ...200719093491
cucurbitane and hexanorcucurbitane glycosides from the fruits of cucurbita pepo cv dayangua.phytochemical investigation of the fruits of cucurbita pepo cv dayangua has led to the isolation of two cucurbitane glycosides: cucurbitacin l 2-o-beta-d-glucopyranoside (1), cucurbitacin k 2-o-beta-d-glucopyranoside (2) and two hexanorcucurbitane glycosides: 2,16-dihydroxy-22,23,24,25,26,27-hexanorcucurbit-5-en-11,20-dione 2-o-beta-d-glucopyranoside (3) and 16-hydroxy-22,23,24,25,26,27-hexanorcucurbit-5-en-11,20-dione 3-o-alpha-l-rhamnopyranosyl-(1 --> 2)-beta-d-glucopyranoside (4). compounds 1 ...200917885839
reversible change of extracellular ph at the generation of mechano-induced electrical reaction in a stem of cucurbita was shown recently the generation of cooling-induced action potential accompanies the reversible change of extracellular ph.1 the ph changes are linked to the ca(2+)-induced transient inhibition of the plasma membrane h(+)-atpase. generation of mechano-induced local bioelectrical reaction (lber) also accompanies the reversible change of extracellular ph. shifts of ph medium during generation of mechano-induced electrical impulse are less than during generation of cooling-induced bioelectrical ...200719704676
surfactants differentially impact p,p'-dde accumulation by plant and earthworm species.the effect of four surfactants (triton x-100, tween-80, rhamnolipids, cyclodextrin) at 100-1000 mg/l on p,p'-dde phytoextraction by cucurbita pepo (zucchini) under field conditions and p,p'-dde bioaccumulation by earthworm species (eisenia fetida, lumbricus terrestris) under laboratory conditions was investigated. abiotically, surfactants (except cyclodextrin) increased contaminant desorption from soil by 4-fold, with higher concentrations generally promoting greater release. cyclodextrin had no ...200717533859
characterization of esterases from cucurbita pepo cv. "eskandrani".two of the six esterases identified in cucurbita pepo cv. "eskandrani" were purified to homogeneity using two chromatography steps: anion exchange and gel filtration. the molecular weights of c. pepo esterases eic and eii were 50,000 +/- 1500 and 68,000 +/- 1900 da from gel filtration and 47,000 and 66,000 da from sds/page, respectively, suggesting a monomeric structure for both enzymes. esterases eic and eii had k(m) values of 1.22 and 1.56 mm and ph optima at 9.0 and 8.0, respectively. the sub ...200817321740
in vitro antioxidant properties of cucurbita pepo l. male and female flowers antioxidant capacities, 2,2-diphenyl-1-picrylhydrazyl (dpph.), hydroxyl (ho.), scavenging activities, and total phenolic values were determined in extracts of cucurbita pepo l. female and male flowers. powdered c. pepo l. samples were extracted in aqueous ethyl acetate (ea: w1, 17:3), ethanol (e), and water (w) by agitating in magnetic stirrer for 80 degrees c, 15 min and also by in aqueous ethyl acetate (ea: w2, 17:3) at 25 degrees c, 15 min. dpph., ho. scavenging capacities and total phe ...200717308962
in situ phytoextraction of polychlorinated biphenyl - (pcb)contaminated soil.a pilot-scale field trial of phytoextraction of pcbs provides insight into the practical application of this technology, using the plant species cucurbita pepo ssp pepo cv. howden (pumpkin), carex normalis (sedge), and festuca arundinacea (tall fescue). this in situ trial took place at a historically contaminated field site, in soil contaminated with a mean concentration of 46 microg/g (range of 0.6 - 200 microg/g) total pcbs (aroclor 1254/1260). shoot bioaccumulation factors (where baf(shoot)=[ ...200717258285
comparing contributions of soil versus root colonization to variations in stomatal behavior and soil drying in mycorrhizal sorghum bicolor and cucurbita prior studies we learned that colonization of soil can be as important as colonization of roots in determining mycorrhizal influence on the water relations of host plants. here we use a path analysis modeling approach to test (a) whether quantity of hyphae in soil contributes to variations in stomatal behavior and soil drying, and (b) whether soil colonization or root colonization has a stronger influence on these stomatal and soil drying responses. experiments were performed on sorghum bicol ...200717189660
reactions, characterization and uptake of ammoxidized kraft lignin labeled with 15n.ammoxidation of kraft lignin was carried out in a parr reactor using (15)nh(3) as the main nitrogen source. reaction parameters were set up until a total nitrogen content of approximately 13 wt.% in lignin was achieved, in accordance with conditions of previous studies. analytical tools such as ftir, py-gc/ms, and solid state nmr were used in this research. the nature of nitrogen bondings is discussed. the incorporation of the (15)n from ammoxidized lignin was followed in pumpkins (zucchini cucu ...200717161600
isotopic enrichment in a phloem-feeding insect: influences of nutrient and water availability.the isotopic enrichment between an animal and its diet can vary among and within living systems, but the sources of variation are not yet fully understood. some studies have found that diet quality or an animal's nutritional status can influence the degree of trophic enrichment, while others have dismissed nutrition as a contributing factor. we evaluated the effects of nutrient and water availability on carbon and nitrogen isotopic enrichment in a specialized plant-herbivore system. aphids are l ...200717124569
growth conditions impact 2,2-bis(p-chlorophenyl)-1,1-dichloroethylene (p,p'-dde) accumulation by cucurbita pepo.laboratory experiments were conducted to study the effects of soil moisture content, planting density, plant age, and the growth of multiple generations on the bioconcentration of weathered p,p'-dde by the plant cucurbita pepo. as soil moisture content increased from 7.4% to 29.9% (by weight), rates of contaminant accumulation by plant roots were increased by more than a factor of 2. higher planting density also led to higher uptake, as the root bioconcentration factor (bcf, dry-weight ratio of ...200617120529
ultrasonic treatment stimulates multiple shoot regeneration and explant enlargement in recalcitrant squash cotyledon explants in vitro.ultrasonic treatment (0.5-2 min) stimulated multiple shoot regeneration to high levels in vitro from recalcitrant cotyledon explants of commercial squash (cucurbita pepo l.) cultivars ma'yan and bareqet, on murashige and skoog [physiol plant 15:473-497, 1962] (regeneration) medium augmented with 4.4 microm benzyladenine. at this stage, unsonicated control explants regenerated only a few very small shoots or bud-like structures. ultrasound also stimulated massive explant growth. ultrasound treatm ...200717021848
methyl jasmonate is a more effective senescence-promoting factor in cucurbita pepo (zucchini) cotyledons when compared with darkness at the early stage of senescence.the effects of short-term darkening and methyl jasmonate (meja) on cotyledon senescence were studied 24h after transfer of intact 7-day-old cucurbita pepo (zucchini) seedlings to darkness or spraying with 100 microm meja. the jasmonate inhibitory effect on chlorophyll content and chloroplast transcriptional activity was stronger compared with darkness. further, meja reduced the photosynthetic rate whereas darkness did not affect photosynthesis. neither stress factor affected the photochemical qu ...200716987568
nectar resorption and translocation in cucurbita pepo l. and platanthera chlorantha custer (rchb.).nectar resorption and sugar translocation were studied in cucurbita pepo (cucurbitaceae) and platanthera chlorantha (orchidaceae) by micro-autoradiography. in both species, nectar was resorbed in pollinated and unpollinated flowers and ovules developing into seeds were found to be the main sugar sink. in c. pepo, the mobility of resorbed sugars in pollinated female flowers was higher than in unpollinated ones; male flowers showed lower mobility of resorbed sugar. in p. chlorantha, radioactivity ...200716883483
immunocytochemical localization of glutathione precursors in plant plant cells, the antioxidant glutathione is synthesized out of its constituents cysteine, glutamate and glycine. as information about their subcellular and cellular distribution was not available in the past, the aim of the present study was to develop a technique that would allow visualization of their distribution in plant cells. with selective antibodies against cysteine, glutamate and glycine, it was possible, for the first time, to study the relative distribution of these components in m ...200616861702
effect of mycorrhizal fungi on the phytoextraction of weathered p,p-dde by cucurbita pepo.field experiments were conducted to assess the impact of inoculation with mycorrhizal fungi on the accumulation of weathered p,p'-dde from soil by three cultivars of zucchini (cucurbita pepo spp. pepo cv costata romanesco, goldrush, raven). three commercially available mycorrhizal products (biovam, myco-vam, invam) were inoculated into the root system of the zucchini seedlings at planting. in agreement with our previous findings, plants not inoculated with fungi accumulated large but variable am ...200616777321
soil amendments, plant age, and intercropping impact p,p'-dde bioavailability to cucurbita pepo.field experiments were conducted to optimize the phytoextraction of weathered p,p'-dde (p,p'-dichlorodiphenyldichloroethylene) by cucurbita subspecies. the effects of two soil amendments, mycorrhizae or a biosurfactant, on p,p'-dde accumulation was determined. also, p,p'-dde uptake was assessed during plant growth (12, 26, 38, and 62 d), and cultivars that accumulate weathered p,p'-dde were intercropped with cultivars known not to have that ability. cucurbita pepo l. ssp. pepo accumulated large ...201316738383
first known image of cucurbita in europe, 1503-1508.the genus cucurbita (pumpkin, squash, gourd) is native to the americas and diffused to other continents subsequent to the european contact in 1492. for many years, the earliest images of this genus in europe that were known to cucurbit specialists were the two illustrations of c. pepo pumpkins that were published in fuchs' de historia stirpium, 1542. images of fruits of two cucurbita species, drawn between 1515 and 1518, were recently discovered in the villa farnesina in rome.200616687431
monitoring presence and advance of the alien invasive western corn rootworm beetle in eastern slovenia with highly sensitive metcalf traps.the american chrysomelid beetle diabrotica virgifera virgifera leconte (d.v.v.), also called the western corn rootworm, spread from the location of its original introduction into europe, belgrade airport (baca 1993), in all directions. within a decade it occupied almost all countries of south-eastern and central europe. however, it reached slovenia as late as 2003: only 19 specimen were found in maize fields of the eastern and also western provinces. already in the summer of 2004, their number h ...200516628904
uptake by cucurbitaceae of soil-bome contaminants depends upon plant genotype and pollutant properties.three cucurbitaceae, cucurbita pepo l. subsp. pepo (cv. black beauty, true zucchini), cucurbita pepo l. intersubspecific cross (cv. zephyr, summer squash), and cucumis sativis (cv. marketmore, cucumber), were grown in rhizotrons containing soil contaminated with three classes of highly weathered, hydrophobic organic contaminants: (1) technical chlordane, (2) dichlorodiphenylethanes (ddt and ddd) and -ethene (dde), (3) polyaromatic hydrocarbons (pahs), and heavy metal residues. movement of the co ...200616570602
intracellular adaptations of glutathione content in cucurbita pepo l. induced by treatment with reduced glutathione and buthionine sulfoximine.the intracellular effects of gsh (reduced glutathione) and bso (buthionine sulfoximine) treatment on glutathione content were investigated with immunogold labeling in individual cellular compartments of cucurbita pepo l. seedlings. generally, gsh treatment led to increased levels of glutathione in roots and leaves (up to 3.5-fold in nuclei), whereas bso treatment significantly decreased glutathione content in all organs. transmission electron microscopy revealed that glutathione levels in mitoch ...200616520878
selenium species determination in selenium-enriched pumpkin (cucurbita pepo l.) seeds by hplc-uv-hg-afs.pumpkins were treated by spraying the leaves in the flowering period with a water solution containing 1.5 mg se per liter in the form of na2seo4. the average total selenium content of seeds was found to be 0.19 microg g(-1) in nontreated pumpkins and 1.1 microg g(-1) in exposed ones. for speciation analysis, enzymatic hydrolysis with different amounts of protease xiv was carried out. under optimal conditions of enzymatic hydrolysis, 90% of the total selenium was found in soluble forms. separatio ...200516379394
cucurbitosides f-m, acylated phenolic glycosides from the seeds of cucurbita pepo.eight new phenolic glycosides, cucurbitosides f-m (1-8), were isolated from the seeds of cucurbita pepo. their structures were elucidated as 4-(2-hydroxyethyl)phenyl 5-o-(2-s-2-methylbutyryl)-beta-d-apiofuranosyl(l-->2)-beta-d-glucopyranoside (1), 4-(2-hydroxyethyl)phenyl 5-o-(3-methylbutyryl)-beta-d-apiofuranosyl(l-->2)-beta-d-glucopyranoside (2), 4-(2-hydroxyethyl)phenyl 5-o-nicotinyl-beta-d-apiofuranosyl(l-->2)-beta-d-glucopyranoside (3), 4-(2-hydroxyethyl)phenyl 5-o-(4-aminobenzoyl)-beta-d-a ...200516378368
ascorbic acid oxidase is dynamically regulated by light and oxygen. a tool for oxygen management in plants?ascorbic acid oxidase (aao) is a plant blue-copper protein catalyzing dioxygen reduction to water using ascorbic acid as the electron donor. in spite of extensive molecular characterization the physiological role of aao is still uncertain. abundant mrna, protein and activity of aao were observed in illuminated leaves of cucurbita pepo. aao activity was found to be proportional to light intensity. the light effect was rapidly reversed in dark and activity remained low throughout the dark period. ...200716343690
dual role of the pigmentation gene b in affecting carotenoid and vitamin e content in squash (cucurbita pepo) mesocarp.the yellow and orange colorations of the mesocarp of pumpkins and squash of cucurbita pepo are due to the presence of carotenoid pigments that also greatly contribute to their nutritional value. carotenoids, as well as tocopherols (vitamin e), are isoprenoid compounds formed by branches of a common biosynthetic pathway. photodiode array hplc analysis was used to simultaneously determine the content and composition of fruit-flesh carotenoids and tocopherols in five pairs of near-isogenic lines di ...200516332127
influence of uv-b exclusion and selenium treatment on photochemical efficiency of photosystem ii, yield and respiratory potential in pumpkins (cucurbita pepo l.).the influence of ambient and filtered solar ultraviolet-b (uv)-b radiation and of selenium treatment was determined on photochemical efficiency, respiratory potential measured by electron transport system (ets) activity, and yield in pumpkins, cucurbita pepo l. yield and ets activity were higher when solar uv-b was filtered out. the results suggested that the decreased yield was related to the uv-b impaired flow of electrons in the respiratory chain. selenium increased yield under ambient radiat ...200515949721
changes in the ascorbate system in the response of pumpkin (cucurbita pepo l.) roots to aluminium stress.the involvement of the ascorbate (asa) system in the response of pumpkin (cucurbita pepo l.) roots to aluminium stress was studied. the treatment of 5-day-old pumpkin seedlings with 50 microm aluminium sulphate resulted in approximately 60% inhibition of root growth within 48-60 h of treatment, while aluminium accumulated in the roots reaching a maximum within 48h. during the same period, the hydrogen peroxide content of the roots was strongly enhanced. the increased level of hydrogen peroxide w ...200515940870
structural reorganization of the copper binding site involving thr15 of mavicyanin from cucurbita pepo medullosa (zucchini) upon reduction.mavicyanin, a glycosylated protein isolated from cucurbita pepo medullosa (zucchini), is a member of the phytocyanin subfamily containing one polypeptide chain of 109 amino residues and an unusual type-i cu site in which the copper ligands are his45, cys86, his91, and gln96. the crystal structures of oxidized and reduced mavicyanin were determined at 1.6 and 1.9 a resolution, respectively. mavicyanin has a core structure of seven polypeptide beta-strands arranged as a beta-sandwich organized int ...200515858169
inheritance of parthenocarpy in summer squash (cucurbita pepo l.).the inheritance of the tendency to set parthenocarpic fruit in the summer squash (cucurbita pepo l.) line whitaker was studied. two parental lines, whitaker (parthenocarpic) and caserta (non-parthenocarpic), and the f1 and f2 generations and backcrosses to both parents were tested. the parthenocarpic tendency of individual plants was scored on a scale from 1 (non-parthenocarpic fruit) to 5 (parthenocarpic fruit). the whitaker line produced parthenocarpic fruit and had a mean score of 4.2, wherea ...200515841434
multigenerational effects of inbreeding in cucurbita pepo ssp. texana (cucurbitaceae).the shape of the fitness function relating the decline in fitness with coefficient of inbreeding (f) can provide evidence concerning the genetic basis of inbreeding depression, but few studies have examined inbreeding depression across a range of f using noncultivated species. futhermore, studies have rarely examined the effects of inbreeding depression in the maternal parent on offspring fitness. to estimate the shape of the fitness function, we examined the relationship between f and fitness a ...200515807414
degradation of aromatic compounds in plants grown under aseptic conditions.the aim of the work is to investigate the ability of higher plants to absorb and detoxify environmental pollutants - aromatic compounds via aromatic ring cleavage. transformation of 14c specifically labelled benzene derivatives, [1-6-14c]-nitrobenzene, [1-6-(14)c]-aniline, [1-(14)c]- and [7-(14)c]-benzoic acid, in axenic seedlings of maize (zea mays l.), kidney bean (phaseolus vulgaris l.), pea (pisum sativum l.) and pumpkin (cucurbita pepo l.) were studied. after penetration in plants, the abov ...201515787252
a zucchini-peroxidase biosensor applied to dopamine determination.a biosensor modified with peroxidase from crude extract of zucchini (cucurbita pepo) was developed for the determination of dopamine in pharmaceutical samples. this enzyme catalyses the oxidation of dopamine to dopaminequinone, in presence of hydrogen peroxide, which the electrochemical reduction can be followed at a peak potential of -0.02 v. the recovery of dopamine from the samples ranged from 94.8% to 106% and a rectilinear analytical curve for dopamine concentration from 5.0 x 10(-4) to 3.0 ...200515752477
environmental variation influences the magnitude of inbreeding depression in cucurbita pepo ssp. texana (cucurbitaceae).we grew inbred and outcrossed cucurbita pepo ssp. texana plants and measured inbreeding depression for several male and female fitness traits 4 years in a row in adjacent fields at the same field station under the same cultivation conditions. we found that the magnitude of inbreeding depression varied from 0.16 to 0.53 from year to year and that those traits which were most affected tended to vary with year. we also grew inbred and outcrossed c. pepo ssp. texana plants in two adjacent fields dif ...200515669971
impact of bemisia argentifolii (homoptera: auchenorrhyncha: aleyrodidae) infestation and squash silverleaf disorder on zucchini yield and quality.fruit yield and quality of zucchini, cucurbita pepo l., plants infested with bemisia argentifolii bellows & perring were evaluated in a screenhouse under spring and fall growing conditions by using closely related sister lines that were either susceptible (zuc61) or tolerant (zuc76-slr) to squash silverleaf disorder. our objective was to test separately the effects of level of whitefly infestation and expression of silverleaf symptoms on zucchini yield and quality. in a second experiment, yield ...200415666769
a comparison of male and female responses to inbreeding in cucurbita pepo subsp. texana (cucurbitaceae).accurate estimates of inbreeding depression are necessary in order to predict the evolutionary dynamics of a population, but many studies estimate inbreeding depression based solely on components of female function such as fruit set, seed set, and seed quality. because total fitness is achieved through both male and female functions in hermaphroditic plants, estimates of both male and female fitness are needed to estimate accurately the magnitude of inbreeding depression. seedlings of a wild gou ...200521652390
uptake of weathered ddt in vascular plants: potential for phytoremediation.since the discovery of its insecticidal properties and its subsequent widespread use, ddt [2,2-bis(chlorophenyl)-1,1,1-trichloroethane] has accumulated in the environment, having a wide range of adverse effects on nontarget species. due to their hydrophobicity, ddt and other persistent organic pollutants are difficult to remove from contaminated soils, and increasingly so through time as weathering occurs. phytoremediation is an emerging plant-based technology that may be used to cost-effectivel ...200415573619
light, conventional and environmental scanning electron microscopy of the trichomes of cucurbita pepo subsp. pepo var. styriaca and histochemistry of glandular secretory the present study, the differences between glandular and non-glandular trichomes, the secretory process and the method of secretion were studied. previous studies on leaves of styrian oil pumpkin (cucurbia pepo var. styriaca) plants have shown that four morphologically and ontogenetically independent glandular and non-glandular trichome types and one bristle hair type can be distinguished. the four types of trichomes can be categorized into three glandular trichome types: type i, a short-stal ...200415306562
influence of bemisia argentifolii (homoptera: aleyrodidae) infestation and squash silverleaf disorder on zucchini seedling growth.we investigated the effect of different levels of infestation by whiteflies, bemisia argentifolii bellows & perring, on the growth and pigment concentrations of seedlings of zucchini, cucurbita pepo l., that differed in their tolerance to squash silverleaf disorder. genetically similar sister lines that were either tolerant (zuc76-slr) or susceptible (zuc61) to silverleaf disorder exhibited reduced plant height, internode length, plant dry weight, and petiole length in response to whitefly feedi ...200415279296
inbreeding influences herbivory in cucurbita pepo ssp. texana (cucurbitaceae).in a series of field experiments diabrotica beetle herbivory was found to influence the magnitude of inbreeding depression in cucurbita pepo ssp. texana, an annual monoecious vine. beetles damage flowers and fruits and chew dime-sized holes in leaf tissue between major veins. inbred plants were found to be more likely to be damaged by beetles and to have more leaves damaged per plant than outcrossed plants. a positive linear association was found between the coefficient of inbreeding and the mag ...200415252728
ultrastructural localization of glutathione in cucurbita pepo plants.electronmicroscopic immunogold cytochemistry was used to investigate the cellular and subcellular distribution of glutathione in root and leaf cells of styrian pumpkin (cucurbita pepo l. subsp. pepo var. styriaca greb.) plants. gold particles bound to glutathione were found in various cell structures. statistical evaluation of the gold particle density was made for different cell compartments including nuclei, mitochondria, plastids, peroxisomes, and the cytosol. in each cell type the highest le ...200415221527
changes in dna methylation during somatic embryogenesis in cucurbita pepo l.three pumpkin embryogenic lines were initiated on wounded zygotic embryos cultured on medium with or without 2,4-dichlorophenoxyacetic acid (2,4-d). somatic embryo development was controlled by the availability of various compounds in the medium: presence/absence of 2,4-d, nitrogen sources. the highest rate of dna methylation was in the early embryo stages, predominantly on msc medium with 2,4-d and on auxin-free medium supplemented with 1.0 m m nh(4)cl. dna methylation was correlated with early ...200415221278
somatic embryogenesis in pumpkin (cucurbita pepo l.): control of somatic embryo development by nitrogen compounds.embryogenic cultures of pumpkin (cucurbita pepo l.) were initiated from mechanically wounded mature zygotic embryos on 2,4-d-containing ms medium, and on hormone-free, semisolid modified ms medium containing nh4cl as the sole source of nitrogen. the habituated line was derived from the embryogenic tissue induced with 2,4-d and maintained on medium without growth regulators. sustained subculturing of the three embryogenic lines on a medium with nh4cl as the sole source of nitrogen enabled the est ...200415022838
in vivo monitoring of chlorophyll fluorescence response to low-dose gamma-irradiation in pumpkin (cucurbita pepo) leaves.the effect of gamma-irradiation on the in vivo-measured chlorophyll fluorescence in a pumpkin leaves (cucurbita pepo) has been investigated. plants were grown in the same environment, then divided into several groups and irradiated at ambient conditions at small dose levels (up to 13.4 gy) with (60)co gamma-rays. the post-irradiation effect on chlorophyll status in the leaves was examined by measuring chlorophyll fluorescence 2 days (48 h) after exposure. it is undoubtedly found that the value o ...200614587079
subspecies-level variation in the phytoextraction of weathered p,p'-dde by cucurbita pepo.previous studies indicate that cucurbita pepo can phytoextract highly weathered persistent organic pollutants (pops) from soil and translocate large quantities to aerial tissues. to investigate intraspecific variability in uptake potential, a field study was conducted to quantify the phytoextraction of weathered p,p'-dde by 21 cultivar varieties of summer squash from two distinct subspecies, c. pepo ssp texana and c. pepo ssp pepo. significant differences exist between the two subspecies, with a ...200314572087
dithiol oxidant and disulfide reductant dynamically regulate the phosphorylation of light-harvesting complex ii proteins in thylakoid membranes.light-induced phosphorylation of light-harvesting chlorophyll a/b complex ii (lhcii) proteins in plant thylakoid membranes requires an activation of the lhcii kinase via binding of plastoquinol to cytochrome b(6)f complex. however, a gradual down-regulation of lhcii protein phosphorylation occurs in higher plant leaves in vivo with increasing light intensity. this inhibition is likely to be mediated by increasing concentration of thiol reductants in the chloroplast. here, we have determined the ...200312970473
irreversibility of chilling injury in zucchini squash (cucurbita pepo l) could be a programmed event long before the visible symptoms are evident.zucchini fruits were subjected to 2.5 or 10 degrees c for 16d, followed by transfer to 20 degrees c for 24h in order to evaluate the relationship between ripening pattern, measured as co(2) evolution and ethylene (c(2)h(4)) production, and metabolic heat production (q). chilling injury (ci) visible symptoms were evident after 8d at 2.5 degrees c, but none were recorded on fruits kept at 10 degrees c. in fruits held at 10 degrees c, q, c(2)h(4) production, and co(2) evolution diminished in the co ...200312893258
genetic diversity of a germplasm collection of cucurbita pepo using srap and aflp markers.cucurbita pepo is a highly polymorphic species. the cultivars can be grouped into eight morphotypes in two subspecies, ssp. pepo and ssp. ovifera. a collection of 69 accessions representative of the morphotypes and some unclassified types was used for analysing the morphological and molecular diversity of this species. this collection includes commercial cultivars and spanish landraces, which represent the great diversification of types that have arisen in europe after this species arrived from ...200312845442
pollen performance before and during the autotrophic-heterotrophic transition of pollen tube growth.for species with bicellular pollen, the attrition of pollen tubes is often greatest where the style narrows at the transition between stigmatic tissue and the transmitting tissue of the style. in this region, the tubes switch from predominantly autotrophic to predominantly heterotrophic growth, the generative cell divides, the first callose plugs are produced, and, in species with rnase-type self-incompatibility (si), incompatible tubes are arrested. we review the literature and present new find ...200312831466
peroxisomal ascorbate peroxidase resides within a subdomain of rough endoplasmic reticulum in wild-type arabidopsis cells.previously we reported (r.t. mullen, c.s. lisenbee, j.a. miernyk, r.n. trelease [1999] plant cell 11: 2167-2185) that overexpressed ascorbate peroxidase (apx), a peroxisomal membrane protein, sorted indirectly to bright yellow-2 cell peroxisomes via a subdomain of the endoplasmic reticulum (er; peroxisomal endoplasmic reticulum [per]). more recently, a per-like compartment also was identified in pumpkin (cucurbita pepo) and transformed arabidopsis cells (k. nito, k. yamaguchi, m. kondo, m. hayas ...200312805617
shoot production in squash (cucurbita pepo) by in vitro organogenesis.seedling-derived cotyledon explants of squash ( cucurbita pepo l.) of commercial cultivars true french, ma'yan and goldy were regenerated in vitro on murashige and skoog medium augmented with 1 mg/l benzyladenine. after 4 weeks in culture small shoots and buds regenerated only on the most proximal cotyledon edge. culture on an elongation medium with a reduced cytokinin concentration (0.1 mg/l) with or without 1 mg/l gibberellic acid (ga(3)) facilitated the recovery of shoots. fresh shoots could ...200312789517
effect of various temperatures on phosphatidylglycerol biosynthesis in thylakoid membranes.lipid unsaturation, the major factor to maintain thylakoid membrane fluidity, is affected by temperature. in this work, we analysed the molecular species composition of phosphatidylglycerol (pg) in thylakoid membranes during spinach (spinacia oleracea) and squash (cucurbita pepo) cotyledon growth to investigate how the growth temperature affects the pg biosynthesis. of the 10 molecular species detected, temperature affected mainly the relative content of molecular species containing linolenic ac ...200312702014
role of organic acids in enhancing the desorption and uptake of weathered p,p'-dde by cucurbita pepo.experiments were conducted to assess the effect of seven organic acids [succinic, tartaric, malic, malonic, oxalic, citric, ethylene-diaminetetraacetic (edta)] over a concentration range of two orders of magnitude (0.001-0.10 m) on the abiotic desorption of weathered p,p'-dde and the extraction of polyvalent inorganic ions from soil. at 0.05 m all organic acids significantly increased contaminant desorption by 19-80%. organic acids also increased the aqueous concentration of eight inorganic cons ...200312683984
assessment of genetic relationships in cucurbita pepo (cucurbitaceae) using dna markers.cucurbita pepo (pumpkin, squash, gourd), an economically important species of the cucurbitaceae, is extremely variable in fruit characteristics. the objective of the present study was to clarify genetic relationships across a broad spectrum of the c. pepo gene pool, with emphasis on domesticates, using aflp, issr and ssr markers. forty-five accessions were compared for presence or absence of 448 aflp, 147 issr, and 20 ssr bands, their genetic distances (gds) were estimated and upgma cluster anal ...200312671744
identification and stereochemical characterization of lignans in flaxseed and pumpkin seeds.phytoestrogens of the lignan type are widely distributed in plant-derived food items and are believed to protect against hormone-dependent cancer. the richest known dietary source of lignans is flaxseed. flaxseed has been reported to contain glycosides of secoisolariciresinol as the major lignan, together with small amounts of matairesinol, isolariciresinol, and pinoresinol. secoisolariciresinol, but none of the other lignans, has so far been identified in pumpkin seeds. in the present study, tw ...200312590454
cell division and cell enlargement in isolated cucurbita cotyledons grown in darkness and in light.the spatial and temporal patterns of post-embryonal cell growth and cell division were characterised in excised cotyledons of vegetable marrow ( cucurbita pepo l. var. giromontia alef.) incubated in water. the concurrent roles of these two processes in cotyledon growth were determined using paradermal sections of the first palisade layer of developing cotyledons. tissue specificity was observed in the pattern of cell division. the daughter cells derived from an initial cell, which had already di ...200212579362
[study on proteins of allele by using the microisoelectric focusing].clear protein electrophoresis pattern of single pollen grain from cucurbita pepo l. was obtained with isoelectric focusing and supersensitive stain technique. the results showed that there were two special distributing protein bands near acidity pole. in species i, the two protein bands were a little near to acidity pole and in species ii, that was a little far from acidity pole. in species iii, the two protein bands distributed either like species i or like species ii and the distributed ratio ...199912552897
short-term boron deprivation inhibits endocytosis of cell wall pectins in meristematic cells of maize and wheat root using immunofluorescence microscopy, we observed rapidly altered distribution patterns of cell wall pectins in meristematic cells of maize (zea mays) and wheat (triticum aestivum) root apices. this response was shown for homogalacturonan pectins characterized by a low level (up to 40%) of methylesterification and for rhamnogalacturonan ii pectins cross-linked by a borate diol diester. under boron deprivation, abundance of these pectins rapidly increased in cell walls, whereas their internaliz ...200212226520
transgenic 14-3-3 isoforms in plants: the metabolite profiling of repressed 14-3-3 protein synthesis in transgenic potato plants.14-3-3 proteins are abundant eukaryotic proteins that interact with many other proteins, thereby modulating their function and thus cell metabolism. the data from mrna analysis confirm the developmental regulation of 14-3-3 isoform expression in potato plants. in order to test whether or not 14-3-3 protein expression affects plant phenotype and metabolism, transgenic potato plants either overexpressing cucurbita pepo 14-3-3 or underexpressing endogenous 14-3-3 isoforms were analysed. an increase ...200212196104
aluminium-induced growth inhibition is associated with impaired efflux and influx of h+ across the plasma membrane in root apices of squash (cucurbita pepo).it is generally understood that the inhibition of growth of root apices is the initial effect caused by aluminium (al) toxicity. the correlation between impaired h+-fluxes across the plasma membrane (pm) and al-induced growth inhibition, al accumulation and callose formation in root apices of squash (cucurbita pepo l. cv. tetsukabuto) is reported here. the root inhibition was dependent on al concentration, and the duration of exposure, with the damage occurring preferentially in regions with hig ...200212177136
quantitative and qualitative analysis of lipids in genetically modified potato tubers with varying rates of 14-3-3 protein six transgenic lines of potatoes with the varying rates of 14-3-3 protein synthesis as well as in control cultivar desiree the content and composition of the lipids extracted from the mature tubers from three years field trials (1998-2000) were analyzed. the transgenic lines j2 and j1 are both overexpressing gene encoding 14-3-3 protein. the j2 exhibited an overexpression of the protein 14-3-3 derived from pumpkin (cucurbita pepo) cdna and in j1 the 14-3-3 overexpression resulted from modifyi ...200212108217
effect of infiltrated polyamines on polygalacturonase activity and chilling injury responses in zucchini squash (cucurbita pepo l.).the effect of exogenous polyamines on electrolyte leakage, chilling index, polygalacturonase activity (pg), ethylene production, and firmness in zucchini squash fruits stored for 12 days at 2 degrees c or 10 degrees c, 85-90% rh was evaluated. fruits were infiltrated with putrescine (put) spermidine (spd) and spermine (spm) at 0.1, 0.25, 0.5, 2.0, and 4.0 mm. all polyamines exerted a protective effect on cell and organelle membranes. the most effective was spd, which reduced electrolyte leakage ...200212083773
screening for effects of phytochemical variability on cytoplasmic protein synthesis pattern of crop plants.crop plants have to cope with phytochemical variability along with other environmental stresses. allelochemicals affect several cellular processes. we tested the effect of toxic aqueous leachates from sicyos deppei, acacia sedillense, sebastiania adenophora, and lantana camara on the radicle growth and cytoplasmic protein synthesis patterns of zea mays (maize), phaseolus vulgaris (bean), cucurbita pepo (squash), and lycopersicon esculentum (tomato). 2d-page and gel scan densitometry analysis wer ...200211944837
cycling of weathered chlordane residues in the environment: compositional and chiral profiles in contiguous soil, vegetation, and air compartments.technical chlordane, a synthetic organic pesticide composed of 147 separate components, some of which exhibit optical activity, was used as an insecticide, herbicide, and termiticide prior to all uses being banned in the united states in 1988. it has been shown that food crops grown in soil treated decades earlier with technical chlordane translocate the weathered chlordane residues from the soil into root and aerial plant tissues. a rigorous analytical method is presented for the simultaneous, ...200211833796
effect of pectic oligomers on physiological responses of chilling injury in discs excised from zucchini (cucurbita pepo l.).the effect of pectic oligomers (og) on ethylene biosynthesis, electrolyte leakage (el), and co(2) production was studied in discs excised from zucchini fruit (cucurbita pepo l.) and stored at 20 or 2.5 degrees c. at 20 degrees c, og enhanced ethylene biosynthesis and had a transient effect on decreasing el, but showed little effect on respiratory rate; both the amount and size of the oligomer were important in changing both ethylene synthesis and el. at 2.5 degrees c, og increased both ethylene ...200211779211
fecundity of transgenic wild-crop hybrids of cucurbita pepo (cucurbitaceae): implications for crop-to-wild gene flow.hybridization between crops and their weedy or wild relatives is an area of concern because the widespread use of genetically engineered crops may allow novel, beneficial transgenes to enter nearby populations. we compared fitness components of wild cucurbita pepo from arkansas, usa, with wild-crop hybrids derived from yellow squash (a cultivar of c. pepo with transgenic resistance to two viruses). wild and hybrid progeny were grown in agricultural fields in arkansas (1996-98) and ohio (1996) in ...200111595050
increase in lipid content in potato tubers modified by 14-3-3 gene overexpression.recently, transgenic potato plants were created with overexpression of the 14-3-3 protein derived from cucurbita pepo. detailed analysis of those plants suggested that the function of the isolated 14-3-3 isoform is in the control of carbohydrate and lipid metabolism in the plants. 14-3-3 protein overexpression gave rise to an increase in soluble sugar and catecholamine contents in both leaves and tubers. it is proposed that 14-3-3 protein affects carbohydrate metabolism in potato plants via regu ...200111513640
aluminum inhibits the h(+)-atpase activity by permanently altering the plasma membrane surface potentials in squash roots.although aluminum (al) toxicity has been widely studied in monocotyledonous crop plants, the mechanism of al impact on economically important dicotyledonous plants is poorly understood. here, we report the spatial pattern of al-induced root growth inhibition, which is closely associated with inhibition of h(+)-atpase activity coupled with decreased surface negativity of plasma membrane (pm) vesicles isolated from apical 5-mm root segments of squash (cucurbita pepo l. cv tetsukabuto) plants. high ...200111500538
uv-c irradiation reduces microbial populations and deterioration in cucurbita pepo fruit tissue.tissue slices of zucchini squash (cucurbita pepo l., cv. tigress) fruit were exposed to ultraviolet-c (uv-c) radiation from germicidal lamps for 1, 10 or 20 min; however, only 10 and 20 min uv-c exposure significantly reduced microbial activity and deterioration during subsequent storage at 5 or 10 degrees c. uv-c treated slices had higher respiration rates than controls; however, the ethylene production of the slices was not affected by uv-c treatments. slight uv-c irradiation damage (reddish b ...200111165626
quiescent intense (qi): a gene that affects young but not mature fruit color intensity in cucurbita pepo.jack o'lantern, a cultivar of the pumpkin group of cucurbita pepo that has intense-colored young fruits, was crossed with vegetable spaghetti, a cultivar of the vegetable marrow group that has light-colored young fruits and genotype d/d i-1/i-1 i-2/i-2. the f1 plants, regardless of the direction of the cross, had light-colored young fruits. young fruit color was observed to segregate in accordance with a 3 intense: 13 light ratio in the f2, indicating that two genes, one dominant and one recessi ...200010912682
african cucurbita pepo l.: properties of seed and variability in fatty acid composition of seed oil.pumpkin (cucurbita pepo) seeds are used locally in eritrea to treat tapeworm. seeds were found to be rich in oil (approximately 35%), protein (38%), alpha-tocoferols (3 mg/100 g) and carbohydrate content (approximately 37%). the physico-chemical properties and fatty acid composition of the seed oil were examined. the four dominant fatty acids found are: palmitic c16:0 (13.3%), stearic c18:0 (8.0%), oleic c18:1 (29.0%) and linoleic c18:2 (47.0%). the oil contains an appreciable amount of unsatura ...200010846750
purification and characterization of a novel pumpkin short-chain acyl-coenzyme a oxidase with structural similarity to acyl-coenzyme a dehydrogenases.a novel pumpkin (cucurbita pepo) short-chain acyl-coenzyme a (coa) oxidase (acox) was purified to homogeneity by hydrophobic-interaction, hydroxyapatite, affinity, and anion-exchange chromatography. the purified enzyme is a tetrameric protein, consisting of apparently identical 47-kd subunits. the protein structure of this oxidase differs from other plant and mammalian acoxs, but is similar to the protein structure of mammalian mitochondrial acyl-coa dehydrogenase (acdh) and the recently identif ...200010806249
molecular cloning and tissue-specific expression of an anionic peroxidase in zucchini.a calcium-pectate-binding anionic isoperoxidase (aprx) from zucchini (cucurbita pepo) was purified and subjected to n-terminal amino acid microsequencing. the cdna encoding this enzyme was obtained by reverse transcriptase polymerase chain reaction from a cdna library. it encoded a mature protein of 309 amino acids exhibiting all of the sequence characteristics of a plant peroxidase. despite the presence of a c-terminal propeptide, aprx was found in the apoplast. aprx protein and mrna were found ...199910398715
effect of tunicamycin on the activity and immunoreactivity of ascorbate oxidase (cucurbita pepo medullosa) expressed in cultured green zucchini cells.ascorbate oxidase activity and immunoreactivity were evaluated in crude tissue extracts obtained from callus cell cultures induced by green zucchini sarcocarp and grown in the presence of tunicamycin, a powerful n-glycosylation inhibitor. tunicamycin at 2 or 4 microg ml(-1) blocked cell growth within a couple of weeks, although a sustained cell viability was observed in the same period. a significant inhibition of total protein synthesis was observed at 10 and 15 days of culture time, with a dec ...19989870353
characterization of spermidine binding to solubilized plasma membrane proteins from zucchini hypocotylsin this work [14c]spermidine binding to total proteins solubilized from plasma membrane purified from zucchini (cucurbita pepo l.) hypocotyls was investigated. proteins were solubilized using octyl glucoside as a detergent. specific polyamine binding was thermolabile, reversible, ph dependent with an optimum at ph 8.0, and had a kd value of 5 &mgr;m, as determined by glass-fiber-filter assays. sephadex g-25 m gel-filtration assays confirmed the presence of a spermidine-protein(s) complex with a ...19989662539
structures of the n-linked carbohydrate of ascorbic acid oxidase from zucchini.the n-glycan moiety of ascorbic acid oxidase from zucchini (cucurbita pepo) has been described to be a core-pentasaccharide with a xylose [d'andrea et al. (1988) glycoconjugate j 5:151-7]. ascorbic acid oxidase is sometimes used to characterize antibodies directed against carbohydrate determinants on plant glycoproteins. to prevent misinterpretations of immunological data, the structure of the n-glycan of ascorbic acid oxidase has been reinvestigated. the oligosaccharides were released by almond ...19989530960
the uptake of radionuclides by beans, squash, and corn growing in contaminated alluvial soils at los alamos national laboratory.pinto beans (phaselous vulgaris), sweet corn (zea mays), and zucchini squash (cucurbita pepo) were grown in a field pot study using alluvial floodplain soils contaminated with various radionuclides within los alamos canyon (lac) at los alamos national laboratory, new mexico. soils as well as washed edible (fruit) and nonedible (stems and leaves) crop tissues were analyzed for tritium (3h), cesium (137cs), strontium (90sr), plutonium (238pu and 239,240pu), americium (241am), and total uranium (to ...19989491570
nucleotide triphosphates are required for the transport of glycolate oxidase into peroxisomes.all peroxisomal proteins are nuclear encoded, synthesized on free cytosolic ribosomes, and posttranslationally targeted to the organelle. we have used an in vitro assay to reconstitute protein import into pumpkin (cucurbita pepo) glyoxysomes, a class of peroxisome found in the cotyledons of oilseed plants, to study the mechanisms involved in protein transport across peroxisome membranes. results indicate that atp hydrolysis is required for protein import into peroxisomes; nonhydrolyzable analogs ...19989449847
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