Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| a defect in the yeast plasma membrane urea transporter dur3p is complemented by cpnip1, a nod26-like protein from zucchini (cucurbita pepo l.), and by arabidopsis thaliana delta-tip or gamma-tip. | dur3 encodes the yeast plasma membrane urea transporter and deltadur3 mutants are unable to grow on media containing low concentrations of urea as sole nitrogen source. complementation of the deltadur3 mutant line with expression libraries generated from whole arabidopsis thaliana seedlings or from zucchini (cucurbita pepo l.) vascular tissue yielded numerous lines that had regained the capacity to grow on low urea as sole nitrogen source. analysis of several of these yeast lines revealed that t ... | 2003 | 12860388 |
| crystallization and preliminary x-ray crystallographic studies of mavicyanin from cucurbita pepo medullosa. | mavicyanin isolated from cucurbita pepo medullosa is a glycosylated protein containing a single polypeptide chain of 109 amino-acid residues and is a member of the phytocyanin subclass of cupredoxins. non-glycosylated recombinant mavicyanin, which was expressed in escherichia coli, was crystallized by the hanging-drop vapour-diffusion method with ammonium sulfate as the precipitant at ph 5.5. the crystals belonged to the hexagonal space group p6(1) (or p6(5)), with unit-cell parameters a = 64.0, ... | 2003 | 12876356 |
| plant uptake and translocation of air-borne chlordane and comparison with the soil-to-plant route. | in order to assess fully the impact of persistent organic pollutants (pops) on human health, pollutant exchange at the interface between terrestrial plants, in particular food crops, and other environmental compartments must be thoroughly understood. in this regard, transfers of multicomponent and chiral pollutants are particularly informative. in the present study, zucchini (cucurbita pepo l.) was planted in containerized, uncontaminated soil under both greenhouse and field conditions and expos ... | 2003 | 12892673 |
| involvement of rna2 for systemic infection of cucumber mosaic virus isolated from lily on zucchini squash. | a lily strain of cucumber mosaic virus (lk-cmv) was not able to systemically infect zucchini squash (cucurbita pepo), while fny strain of cmv (fny-cmv) caused systemic mosaic and stunting symptom at 4 days post-inoculation on the same host species. the pathogenicity of lk-cmv in zucchini squash was investigated by reassortments of genomic rnas of lk-cmv and fny-cmv for infection, as well as by pseudorecombinants generated from biologically active transcripts of cdna clones of lk-cmv and fny-cmv, ... | 2003 | 14550582 |
| synthesis of l-ascorbic acid in the phloem. | although plants are the main source of vitamin c in the human diet, we still have a limited understanding of how plants synthesise l-ascorbic acid (asa) and what regulates its concentration in different plant tissues. in particular, the enormous variability in the vitamin c content of storage organs from different plants remains unexplained. possible sources of asa in plant storage organs include in situ synthesis and long-distance transport of asa synthesised in other tissues via the phloem. in ... | 2003 | 14633288 |
| effect of crotalaria juncea amendment on nematode communities in soil with different agricultural histories. | effect of sunn hemp (crotalaria juncea) hay amendment on nematode community structure in the soil surrounding roots of yellow squash (cucurbita pepo) infected with root-knot nematodes was examined in two greenhouse experiments. soils were from field plots treated long-term (lt) with yard-waste compost or no yard-waste compost in lt experiment, and from a short-term (st) agricultural site in st experiment. soils collected were either amended or not amended with c. juncea hay. nematode communities ... | 2003 | 19262764 |
| melon resistance to cucurbit yellow stunting disorder virus is characterized by reduced virus accumulation. | abstract the pattern of accumulation of cucurbit yellow stunting disorder virus (cysdv; genus crinivirus, family closteroviridae) rna has been analyzed in several cucurbit accessions. in susceptible accessions of melon (cucumis melo), cucumber (cucumis sativus), marrow (cucurbita maxima), and squash (cucurbita pepo), cysdv rna accumulation peaked during the first to second week postinoculation in the first to third leaf above the inoculated one; younger leaves showed very low or undetectable lev ... | 2003 | 18943165 |
| synergic effect studies of the bi-enzymatic system laccase-peroxidase in a voltammetric biosensor for catecholamines. | several bi-enzymatic carbon paste biosensors modified with enzymes laccase from pleurotus ostreatus fungi and peroxidase from zucchini (cucurbita pepo) were constructed for evaluating the synergic effect of the two enzymes on the voltammetric biosensor response for various catecholamines. initially was investigated the effect of ph from 5.0 to 7.5, temperature from 25 to 50 degrees c, initial stirring time from 30 to 150 s, scan rate from 10 to 60 mvs(-1) and potential pulse amplitude from 10 to ... | 2003 | 18968977 |
| subspecies-level variation in the phytoextraction of weathered p,p'-dde by cucurbita pepo. | previous studies indicate that cucurbita pepo can phytoextract highly weathered persistent organic pollutants (pops) from soil and translocate large quantities to aerial tissues. to investigate intraspecific variability in uptake potential, a field study was conducted to quantify the phytoextraction of weathered p,p'-dde by 21 cultivar varieties of summer squash from two distinct subspecies, c. pepo ssp texana and c. pepo ssp pepo. significant differences exist between the two subspecies, with a ... | 2003 | 14572087 |
| dithiol oxidant and disulfide reductant dynamically regulate the phosphorylation of light-harvesting complex ii proteins in thylakoid membranes. | light-induced phosphorylation of light-harvesting chlorophyll a/b complex ii (lhcii) proteins in plant thylakoid membranes requires an activation of the lhcii kinase via binding of plastoquinol to cytochrome b(6)f complex. however, a gradual down-regulation of lhcii protein phosphorylation occurs in higher plant leaves in vivo with increasing light intensity. this inhibition is likely to be mediated by increasing concentration of thiol reductants in the chloroplast. here, we have determined the ... | 2003 | 12970473 |
| irreversibility of chilling injury in zucchini squash (cucurbita pepo l) could be a programmed event long before the visible symptoms are evident. | zucchini fruits were subjected to 2.5 or 10 degrees c for 16d, followed by transfer to 20 degrees c for 24h in order to evaluate the relationship between ripening pattern, measured as co(2) evolution and ethylene (c(2)h(4)) production, and metabolic heat production (q). chilling injury (ci) visible symptoms were evident after 8d at 2.5 degrees c, but none were recorded on fruits kept at 10 degrees c. in fruits held at 10 degrees c, q, c(2)h(4) production, and co(2) evolution diminished in the co ... | 2003 | 12893258 |
| genetic diversity of a germplasm collection of cucurbita pepo using srap and aflp markers. | cucurbita pepo is a highly polymorphic species. the cultivars can be grouped into eight morphotypes in two subspecies, ssp. pepo and ssp. ovifera. a collection of 69 accessions representative of the morphotypes and some unclassified types was used for analysing the morphological and molecular diversity of this species. this collection includes commercial cultivars and spanish landraces, which represent the great diversification of types that have arisen in europe after this species arrived from ... | 2003 | 12845442 |
| pollen performance before and during the autotrophic-heterotrophic transition of pollen tube growth. | for species with bicellular pollen, the attrition of pollen tubes is often greatest where the style narrows at the transition between stigmatic tissue and the transmitting tissue of the style. in this region, the tubes switch from predominantly autotrophic to predominantly heterotrophic growth, the generative cell divides, the first callose plugs are produced, and, in species with rnase-type self-incompatibility (si), incompatible tubes are arrested. we review the literature and present new find ... | 2003 | 12831466 |
| peroxisomal ascorbate peroxidase resides within a subdomain of rough endoplasmic reticulum in wild-type arabidopsis cells. | previously we reported (r.t. mullen, c.s. lisenbee, j.a. miernyk, r.n. trelease [1999] plant cell 11: 2167-2185) that overexpressed ascorbate peroxidase (apx), a peroxisomal membrane protein, sorted indirectly to bright yellow-2 cell peroxisomes via a subdomain of the endoplasmic reticulum (er; peroxisomal endoplasmic reticulum [per]). more recently, a per-like compartment also was identified in pumpkin (cucurbita pepo) and transformed arabidopsis cells (k. nito, k. yamaguchi, m. kondo, m. hayas ... | 2003 | 12805617 |
| shoot production in squash (cucurbita pepo) by in vitro organogenesis. | seedling-derived cotyledon explants of squash ( cucurbita pepo l.) of commercial cultivars true french, ma'yan and goldy were regenerated in vitro on murashige and skoog medium augmented with 1 mg/l benzyladenine. after 4 weeks in culture small shoots and buds regenerated only on the most proximal cotyledon edge. culture on an elongation medium with a reduced cytokinin concentration (0.1 mg/l) with or without 1 mg/l gibberellic acid (ga(3)) facilitated the recovery of shoots. fresh shoots could ... | 2003 | 12789517 |
| effect of various temperatures on phosphatidylglycerol biosynthesis in thylakoid membranes. | lipid unsaturation, the major factor to maintain thylakoid membrane fluidity, is affected by temperature. in this work, we analysed the molecular species composition of phosphatidylglycerol (pg) in thylakoid membranes during spinach (spinacia oleracea) and squash (cucurbita pepo) cotyledon growth to investigate how the growth temperature affects the pg biosynthesis. of the 10 molecular species detected, temperature affected mainly the relative content of molecular species containing linolenic ac ... | 2003 | 12702014 |
| role of organic acids in enhancing the desorption and uptake of weathered p,p'-dde by cucurbita pepo. | experiments were conducted to assess the effect of seven organic acids [succinic, tartaric, malic, malonic, oxalic, citric, ethylene-diaminetetraacetic (edta)] over a concentration range of two orders of magnitude (0.001-0.10 m) on the abiotic desorption of weathered p,p'-dde and the extraction of polyvalent inorganic ions from soil. at 0.05 m all organic acids significantly increased contaminant desorption by 19-80%. organic acids also increased the aqueous concentration of eight inorganic cons ... | 2003 | 12683984 |
| assessment of genetic relationships in cucurbita pepo (cucurbitaceae) using dna markers. | cucurbita pepo (pumpkin, squash, gourd), an economically important species of the cucurbitaceae, is extremely variable in fruit characteristics. the objective of the present study was to clarify genetic relationships across a broad spectrum of the c. pepo gene pool, with emphasis on domesticates, using aflp, issr and ssr markers. forty-five accessions were compared for presence or absence of 448 aflp, 147 issr, and 20 ssr bands, their genetic distances (gds) were estimated and upgma cluster anal ... | 2003 | 12671744 |
| identification and stereochemical characterization of lignans in flaxseed and pumpkin seeds. | phytoestrogens of the lignan type are widely distributed in plant-derived food items and are believed to protect against hormone-dependent cancer. the richest known dietary source of lignans is flaxseed. flaxseed has been reported to contain glycosides of secoisolariciresinol as the major lignan, together with small amounts of matairesinol, isolariciresinol, and pinoresinol. secoisolariciresinol, but none of the other lignans, has so far been identified in pumpkin seeds. in the present study, tw ... | 2003 | 12590454 |
| uptake of weathered ddt in vascular plants: potential for phytoremediation. | since the discovery of its insecticidal properties and its subsequent widespread use, ddt [2,2-bis(chlorophenyl)-1,1,1-trichloroethane] has accumulated in the environment, having a wide range of adverse effects on nontarget species. due to their hydrophobicity, ddt and other persistent organic pollutants are difficult to remove from contaminated soils, and increasingly so through time as weathering occurs. phytoremediation is an emerging plant-based technology that may be used to cost-effectivel ... | 2004 | 15573619 |
| light, conventional and environmental scanning electron microscopy of the trichomes of cucurbita pepo subsp. pepo var. styriaca and histochemistry of glandular secretory products. | in the present study, the differences between glandular and non-glandular trichomes, the secretory process and the method of secretion were studied. previous studies on leaves of styrian oil pumpkin (cucurbia pepo var. styriaca) plants have shown that four morphologically and ontogenetically independent glandular and non-glandular trichome types and one bristle hair type can be distinguished. the four types of trichomes can be categorized into three glandular trichome types: type i, a short-stal ... | 2004 | 15306562 |
| influence of bemisia argentifolii (homoptera: aleyrodidae) infestation and squash silverleaf disorder on zucchini seedling growth. | we investigated the effect of different levels of infestation by whiteflies, bemisia argentifolii bellows & perring, on the growth and pigment concentrations of seedlings of zucchini, cucurbita pepo l., that differed in their tolerance to squash silverleaf disorder. genetically similar sister lines that were either tolerant (zuc76-slr) or susceptible (zuc61) to silverleaf disorder exhibited reduced plant height, internode length, plant dry weight, and petiole length in response to whitefly feedi ... | 2004 | 15279296 |
| inbreeding influences herbivory in cucurbita pepo ssp. texana (cucurbitaceae). | in a series of field experiments diabrotica beetle herbivory was found to influence the magnitude of inbreeding depression in cucurbita pepo ssp. texana, an annual monoecious vine. beetles damage flowers and fruits and chew dime-sized holes in leaf tissue between major veins. inbred plants were found to be more likely to be damaged by beetles and to have more leaves damaged per plant than outcrossed plants. a positive linear association was found between the coefficient of inbreeding and the mag ... | 2004 | 15252728 |
| ultrastructural localization of glutathione in cucurbita pepo plants. | electronmicroscopic immunogold cytochemistry was used to investigate the cellular and subcellular distribution of glutathione in root and leaf cells of styrian pumpkin (cucurbita pepo l. subsp. pepo var. styriaca greb.) plants. gold particles bound to glutathione were found in various cell structures. statistical evaluation of the gold particle density was made for different cell compartments including nuclei, mitochondria, plastids, peroxisomes, and the cytosol. in each cell type the highest le ... | 2004 | 15221527 |
| changes in dna methylation during somatic embryogenesis in cucurbita pepo l. | three pumpkin embryogenic lines were initiated on wounded zygotic embryos cultured on medium with or without 2,4-dichlorophenoxyacetic acid (2,4-d). somatic embryo development was controlled by the availability of various compounds in the medium: presence/absence of 2,4-d, nitrogen sources. the highest rate of dna methylation was in the early embryo stages, predominantly on msc medium with 2,4-d and on auxin-free medium supplemented with 1.0 m m nh(4)cl. dna methylation was correlated with early ... | 2004 | 15221278 |
| somatic embryogenesis in pumpkin (cucurbita pepo l.): control of somatic embryo development by nitrogen compounds. | embryogenic cultures of pumpkin (cucurbita pepo l.) were initiated from mechanically wounded mature zygotic embryos on 2,4-d-containing ms medium, and on hormone-free, semisolid modified ms medium containing nh4cl as the sole source of nitrogen. the habituated line was derived from the embryogenic tissue induced with 2,4-d and maintained on medium without growth regulators. sustained subculturing of the three embryogenic lines on a medium with nh4cl as the sole source of nitrogen enabled the est ... | 2004 | 15022838 |
| impact of bemisia argentifolii (homoptera: auchenorrhyncha: aleyrodidae) infestation and squash silverleaf disorder on zucchini yield and quality. | fruit yield and quality of zucchini, cucurbita pepo l., plants infested with bemisia argentifolii bellows & perring were evaluated in a screenhouse under spring and fall growing conditions by using closely related sister lines that were either susceptible (zuc61) or tolerant (zuc76-slr) to squash silverleaf disorder. our objective was to test separately the effects of level of whitefly infestation and expression of silverleaf symptoms on zucchini yield and quality. in a second experiment, yield ... | 2004 | 15666769 |
| effect of crotalaria juncea amendment on squash infected with meloidogyne incognita. | two greenhouse experiments were conducted to examine the effect of crotalaria juncea amendment on meloidogyne incognita population levels and growth of yellow squash (cucurbita pepo). in the first experiment, four soils with a long history of receiving yard waste compost (ywc+), no-yard-waste compost (ywc-), conventional tillage, or no-tillage treatments were used; in the second experiment, only one recently cultivated soil was used. half of the amount of each soil received air-dried residues of ... | 2004 | 19262819 |
| movement of coat protein genes from a commercial virus-resistant transgenic squash into a wild relative. | we monitored pollen-mediated transgene dissemination from commercial transgenic squash czw-3 into its wild relative cucurbita pepo ssp. ovifera var. texana (c. texana). transgenic squash czw-3 expresses the neomycin phosphotransferase ii (nptii) gene and the coat protein (cp) genes of cucumber mosaic virus (cmv), zucchini yellow mosaic virus (zymv), and watermelon mosaic virus (wmv); thereby, it is resistant to these three aphid-borne viruses. the rate of npt ii and cp transgene introgression in ... | 2004 | 15612351 |
| comparative fitness of a wild squash species and three generations of hybrids between wild x virus-resistant transgenic squash. | we compared some fitness components of the wild squash species cucurbita pepo spp. ovifera var. texana (c. texana) and three generations of hybrids (f1, bc1, and bc2) between c. texana and commercial transgenic squash czw-3 over three consecutive years under field conditions of low (ldp) and high disease pressure (hdp) by cucumber mosaic virus (cmv), zucchini yellow mosaic virus (zymv) and watermelon mosaic virus (wmv). transgenic squash czw-3 expresses the coat protein (cp) genes of cmv, zymv, ... | 2004 | 15612352 |
| occurrence, distribution and relative incidence of mosaic viruses infecting field--grown squash in tehran province, iran. | squash (cucurbita pepo) belongs to cucurbitaceae family. every year cucurbitaceae are planted world wide. they are one of the most important economic crops. cucurbitaceae are threatened by viruses. many viruses damage the plants of this family. since nine viruses have been reported on squash from iran. in this survey, during 2002--2003, to determine the distribution of cucumber mosaic virus (cmv), zucchini yellow mosaic virus (zymv) and watermelon mosaic virus (wmv), 466 samples were collected f ... | 2004 | 15756831 |
| phytoextraction of weathered p,p'-dde by zucchini (cucurbita pepo) and cucumber (cucumis sativus) under different cultivation conditions. | previous studies have shown that zucchini (cucurbita pepo) and cucumber (cucumis sativus) under field conditions are good and poor accumulators, respectively, of persistent organic pollutants from soil. here, each species was grown under three cultivation regimes: dense (five plants in 5 kg soil): nondense (one plant in 80 kg soil): and field conditions (two to three plants in approximately 789 kg soil). p,p'-dde and inorganic element content in roots, stems, leaves, and fruit were determined. i ... | 2004 | 15696707 |
| plant uptake and translocation of highly weathered, soil-bound technical chlordane residues: data from field and rhizotron studies. | it has been observed that plants are susceptible to uptake from soil and in planta transport of technical chlordane, in spite of its hydrophobicity and sequestration within the soil matrix due to weathering. field and rhizotron studies were conducted with cucurbitaceae planted in highly weathered, chlordane-contaminated soil to investigate details of soil-to-plant contaminant uptake. in the field-work, cucurbita pepo l. (zucchini) was grown in soil at four levels of chlordane contamination: clea ... | 2004 | 15559292 |
| releases of psyttalia fletcheri (hymenoptera: braconidae) and sterile flies to suppress melon fly (diptera: tephritidae) in hawaii. | ivy gourd, coccinia grandis (l.) voigt, patches throughout kailua-kona, hawaii island, hi, were identified as persistent sources of melon fly, bactrocera cucurbitae (coquillett). these patches had a low incidence of psyttalia fletcheri (silvestri), its major braconid parasitoid natural enemy in hawaii, and were used to evaluate augmentative releases of p. fletcheri against melon fly. in field cage studies of releases, numbers of melon flies emerging from ivy gourd fruit placed inside treatment c ... | 2004 | 15568340 |
| brood pheromone regulates foraging activity of honey bees (hymenoptera: apidae). | brood pheromone modulated the foraging behavior of commercial honey bee, apis mellifera l., colonies pollinating a 10-ha market garden of cucumber, cucurbita pepo l., and zucchini, cucumis saticus l., in texas in late autumn. six colonies were randomly selected to receive 2000 larval equivalents of brood pheromone and six received a blank control. the ratio of pollen to nonpollen foragers entering colonies was significantly greater in pheromone-treated colonies 1 h after treatment. pheromone-tre ... | 2004 | 15279247 |
| plant growth, phosphorus nutrition, and root morphological responses to arbuscular mycorrhizas, phosphorus fertilization, and intraspecific density. | we examined the effects of arbuscular mycorrhizas (am), phosphorus fertilization, intraspecific density, and their interaction, on the growth, phosphorus uptake, and root morphology of three facultative mycotrophic crops (capsicum annuum, zea mays, and cucurbita pepo). plants were grown in pots with or without am at three densities and four phosphorus availabilities for 10 weeks. am colonization, plant weight, and shoot phosphorus concentration were measured at harvest. root morphology was asses ... | 2005 | 15316886 |
| influence of nutrient amendments on the phytoextraction of weathered 2,2-bis(p-chlorophenyl)-1,1-dichloroethylene by cucurbits. | field experiments were conducted to determine the impact of nutrient amendments on the phytoextraction of weathered 2,2-bis(p-chlorophenyl)-1,1-dichloroethylene (p,p '-dde) by eight cultivars of cucurbits over a single growing season. four cultivars of cucurbita pepo ssp pepo are accumulators and extract percent level quantities of persistent organic pollutants (pops), whereas c. pepo ssp ovifera and cucumis sativus are nonaccumulators. the nonamended accumulators phytoextracted 1.0% of the p,p' ... | 2005 | 15839575 |
| reassessing coxcatlan cave and the early history of domesticated plants in mesoamerica. | reanalysis and direct accelerator mass spectrometry radiocarbon dating of the cucurbit assemblage from coxcatlan cave provide information on the timing and sequence of the initial appearance of three domesticated plants in the tehuacán valley (puebla, mexico) and allow reassessment of the overall temporal context of plant domestication in mexico. cucurbita pepo is the earliest documented domesticate in the cave, dating to 7,920 calibrated calendrical (cal) years b.p. the bottle gourd (lagenaria ... | 2005 | 15908505 |
| phenolic profiles of raw apricots, pumpkins, and their purees in the evaluation of apricot nectar and jam authenticity. | the possibility of proving the undeclared addition of pumpkin puree in apricot nectars and jams has been investigated by using the phenol compound fingerprint and sensory evaluation. the cheaper pumpkin admixtures in apricot nectars and jams could not be detected by the sensory evaluation, particularly if present in quantities of <15%. the lower admixtures of pumpkin puree in apricot nectars and jams could be detected by the presence of syringic acid, a phenolic compound characteristic of the in ... | 2005 | 15941324 |
| multi-species interactions impact the accumulation of weathered 2,2-bis (p-chlorophenyl)-1,1-dichloroethylene (p,p'-dde) from soil. | the impact of interactions between the earthworms eisenia foetida and lumbricus terrestris and the plants cucurbita pepo and cucurbita maxima on the uptake of weathered p,p'-dde from soil was determined. although some combinations of earthworm and plant species caused significant changes in the p,p'-dde burden in both organisms, the effects were species specific. contaminant bioconcentration in c. pepo was increased slightly by e. foetida and by 3-fold when the plant was grown with l. terrestris ... | 2005 | 15913856 |
| salicylic acid-induced resistance to cucumber mosaic virus in squash and arabidopsis thaliana: contrasting mechanisms of induction and antiviral action. | salicylic acid (sa)-induced resistance to cucumber mosaic virus (cmv) in tobacco (nicotiana tabacum) results from inhibition of systemic virus movement and is induced via a signal transduction pathway that also can be triggered by antimycin a, an inducer of the mitochondrial enzyme alternative oxidase (aox). in arabidopsis thaliana, inhibition of cmv systemic movement also is induced by sa and antimycin a. these results indicate that the mechanisms underlying induced resistance to cmv in tobacco ... | 2005 | 15915641 |
| arabidopsis peroxisomal citrate synthase is required for fatty acid respiration and seed germination. | we tested the hypothesis that peroxisomal citrate synthase (csy) is required for carbon transfer from peroxisomes to mitochondria during respiration of triacylglycerol in arabidopsis thaliana seedlings. two genes encoding peroxisomal csy are expressed in arabidopsis seedlings, and seeds from plants with both csy genes disrupted were dormant and did not metabolize triacylglycerol. germination was achieved by removing the seed coat and supplying sucrose, but the seedlings still did not use triacyl ... | 2005 | 15923350 |
| effect of pumpkin seed (cucurbita pepo) protein isolate on the activity levels of certain plasma enzymes in ccl4-induced liver injury in low-protein fed rats. | the effects of pumpkin seed (cucurbita pepo) protein isolate on the activity levels of lactate dehydrogenase (ld), alanine transaminase (alt), aspartate transaminase (ast) and alkaline phosphatase (alp) against carbon tetrachloride (ccl4)-induced acute liver injury in low-protein fed rats were investigated. a group of male sprague-dawley rats maintained on a low-protein diet for 5 days were divided into three subgroups. two subgroups were injected with carbon tetrachloride and the other group wi ... | 2005 | 16041732 |
| effects of different fixation and freeze substitution methods on the ultrastructural preservation of zymv-infected cucurbita pepo (l.) leaves. | different fixation protocols [chemical fixation, plunge and high pressure freezing (hpf)] were used to study the effects of zucchini yellow mosaic virus (zymv) disease on the ultrastructure of adult leaves of styrian oil pumpkin plants (cucurbita pepo l. subsp. pepo var. styriaca greb.) with the transmission electron microscope. additionally, different media were tested for freeze substitution (fs) to evaluate differences in the ultrastructural preservation of cryofixed plant leaf cells. fs was ... | 2005 | 16123060 |
| comparative study on biological parameters of bemisia tabaci (genn.) collected on four host plants from varamin-iran. | during 2003 biological parameters of sweetpotato whitefly, b. tabaci (genn.) (horn. aleyrodidae) as a major pest of field crops, vegetables and ornamentals were studied. in this study, the infested leaves of cucumber (cucumis sativus l.) zucchini (cucurbita pepo l.) eggplant (solanum melongena l.) and cotton (gossypium hirsutum l.) with whitefly nymphs and pupae were collected from varamin-iran, and were transferred to the laboratory. the newly emerged males and females of each population were r ... | 2005 | 16628901 |
| changes in the subcellular distribution of glutathione during virus infection in cucurbita pepo (l.). | changes in the subcellular distribution and quantification of glutathione were studied with electron microscopic immunogold cytochemistry in zucchini yellow mosaic virus (zymv)-infected styrian pumpkin plants (cucurbita pepo l. ssp. pepo var. styriaca greb.) two weeks after inoculation. the amount of gold particles bound to glutathione was statistically evaluated for different cell structures, including mitochondria, plastids, nuclei, peroxisomes, and cytosol. in general, zymv-infected plants sh ... | 2005 | 15666214 |
| a comparison of male and female responses to inbreeding in cucurbita pepo subsp. texana (cucurbitaceae). | accurate estimates of inbreeding depression are necessary in order to predict the evolutionary dynamics of a population, but many studies estimate inbreeding depression based solely on components of female function such as fruit set, seed set, and seed quality. because total fitness is achieved through both male and female functions in hermaphroditic plants, estimates of both male and female fitness are needed to estimate accurately the magnitude of inbreeding depression. seedlings of a wild gou ... | 2005 | 21652390 |
| a zucchini-peroxidase biosensor applied to dopamine determination. | a biosensor modified with peroxidase from crude extract of zucchini (cucurbita pepo) was developed for the determination of dopamine in pharmaceutical samples. this enzyme catalyses the oxidation of dopamine to dopaminequinone, in presence of hydrogen peroxide, which the electrochemical reduction can be followed at a peak potential of -0.02 v. the recovery of dopamine from the samples ranged from 94.8% to 106% and a rectilinear analytical curve for dopamine concentration from 5.0 x 10(-4) to 3.0 ... | 2005 | 15752477 |
| environmental variation influences the magnitude of inbreeding depression in cucurbita pepo ssp. texana (cucurbitaceae). | we grew inbred and outcrossed cucurbita pepo ssp. texana plants and measured inbreeding depression for several male and female fitness traits 4 years in a row in adjacent fields at the same field station under the same cultivation conditions. we found that the magnitude of inbreeding depression varied from 0.16 to 0.53 from year to year and that those traits which were most affected tended to vary with year. we also grew inbred and outcrossed c. pepo ssp. texana plants in two adjacent fields dif ... | 2005 | 15669971 |
| monitoring presence and advance of the alien invasive western corn rootworm beetle in eastern slovenia with highly sensitive metcalf traps. | the american chrysomelid beetle diabrotica virgifera virgifera leconte (d.v.v.), also called the western corn rootworm, spread from the location of its original introduction into europe, belgrade airport (baca 1993), in all directions. within a decade it occupied almost all countries of south-eastern and central europe. however, it reached slovenia as late as 2003: only 19 specimen were found in maize fields of the eastern and also western provinces. already in the summer of 2004, their number h ... | 2005 | 16628904 |
| selenium species determination in selenium-enriched pumpkin (cucurbita pepo l.) seeds by hplc-uv-hg-afs. | pumpkins were treated by spraying the leaves in the flowering period with a water solution containing 1.5 mg se per liter in the form of na2seo4. the average total selenium content of seeds was found to be 0.19 microg g(-1) in nontreated pumpkins and 1.1 microg g(-1) in exposed ones. for speciation analysis, enzymatic hydrolysis with different amounts of protease xiv was carried out. under optimal conditions of enzymatic hydrolysis, 90% of the total selenium was found in soluble forms. separatio ... | 2005 | 16379394 |
| cucurbitosides f-m, acylated phenolic glycosides from the seeds of cucurbita pepo. | eight new phenolic glycosides, cucurbitosides f-m (1-8), were isolated from the seeds of cucurbita pepo. their structures were elucidated as 4-(2-hydroxyethyl)phenyl 5-o-(2-s-2-methylbutyryl)-beta-d-apiofuranosyl(l-->2)-beta-d-glucopyranoside (1), 4-(2-hydroxyethyl)phenyl 5-o-(3-methylbutyryl)-beta-d-apiofuranosyl(l-->2)-beta-d-glucopyranoside (2), 4-(2-hydroxyethyl)phenyl 5-o-nicotinyl-beta-d-apiofuranosyl(l-->2)-beta-d-glucopyranoside (3), 4-(2-hydroxyethyl)phenyl 5-o-(4-aminobenzoyl)-beta-d-a ... | 2005 | 16378368 |
| dual role of the pigmentation gene b in affecting carotenoid and vitamin e content in squash (cucurbita pepo) mesocarp. | the yellow and orange colorations of the mesocarp of pumpkins and squash of cucurbita pepo are due to the presence of carotenoid pigments that also greatly contribute to their nutritional value. carotenoids, as well as tocopherols (vitamin e), are isoprenoid compounds formed by branches of a common biosynthetic pathway. photodiode array hplc analysis was used to simultaneously determine the content and composition of fruit-flesh carotenoids and tocopherols in five pairs of near-isogenic lines di ... | 2005 | 16332127 |
| influence of uv-b exclusion and selenium treatment on photochemical efficiency of photosystem ii, yield and respiratory potential in pumpkins (cucurbita pepo l.). | the influence of ambient and filtered solar ultraviolet-b (uv)-b radiation and of selenium treatment was determined on photochemical efficiency, respiratory potential measured by electron transport system (ets) activity, and yield in pumpkins, cucurbita pepo l. yield and ets activity were higher when solar uv-b was filtered out. the results suggested that the decreased yield was related to the uv-b impaired flow of electrons in the respiratory chain. selenium increased yield under ambient radiat ... | 2005 | 15949721 |
| changes in the ascorbate system in the response of pumpkin (cucurbita pepo l.) roots to aluminium stress. | the involvement of the ascorbate (asa) system in the response of pumpkin (cucurbita pepo l.) roots to aluminium stress was studied. the treatment of 5-day-old pumpkin seedlings with 50 microm aluminium sulphate resulted in approximately 60% inhibition of root growth within 48-60 h of treatment, while aluminium accumulated in the roots reaching a maximum within 48h. during the same period, the hydrogen peroxide content of the roots was strongly enhanced. the increased level of hydrogen peroxide w ... | 2005 | 15940870 |
| structural reorganization of the copper binding site involving thr15 of mavicyanin from cucurbita pepo medullosa (zucchini) upon reduction. | mavicyanin, a glycosylated protein isolated from cucurbita pepo medullosa (zucchini), is a member of the phytocyanin subfamily containing one polypeptide chain of 109 amino residues and an unusual type-i cu site in which the copper ligands are his45, cys86, his91, and gln96. the crystal structures of oxidized and reduced mavicyanin were determined at 1.6 and 1.9 a resolution, respectively. mavicyanin has a core structure of seven polypeptide beta-strands arranged as a beta-sandwich organized int ... | 2005 | 15858169 |
| multigenerational effects of inbreeding in cucurbita pepo ssp. texana (cucurbitaceae). | the shape of the fitness function relating the decline in fitness with coefficient of inbreeding (f) can provide evidence concerning the genetic basis of inbreeding depression, but few studies have examined inbreeding depression across a range of f using noncultivated species. futhermore, studies have rarely examined the effects of inbreeding depression in the maternal parent on offspring fitness. to estimate the shape of the fitness function, we examined the relationship between f and fitness a ... | 2005 | 15807414 |
| inheritance of parthenocarpy in summer squash (cucurbita pepo l.). | the inheritance of the tendency to set parthenocarpic fruit in the summer squash (cucurbita pepo l.) line whitaker was studied. two parental lines, whitaker (parthenocarpic) and caserta (non-parthenocarpic), and the f1 and f2 generations and backcrosses to both parents were tested. the parthenocarpic tendency of individual plants was scored on a scale from 1 (non-parthenocarpic fruit) to 5 (parthenocarpic fruit). the whitaker line produced parthenocarpic fruit and had a mean score of 4.2, wherea ... | 2005 | 15841434 |
| uptake by cucurbitaceae of soil-bome contaminants depends upon plant genotype and pollutant properties. | three cucurbitaceae, cucurbita pepo l. subsp. pepo (cv. black beauty, true zucchini), cucurbita pepo l. intersubspecific cross (cv. zephyr, summer squash), and cucumis sativis (cv. marketmore, cucumber), were grown in rhizotrons containing soil contaminated with three classes of highly weathered, hydrophobic organic contaminants: (1) technical chlordane, (2) dichlorodiphenylethanes (ddt and ddd) and -ethene (dde), (3) polyaromatic hydrocarbons (pahs), and heavy metal residues. movement of the co ... | 2006 | 16570602 |
| intracellular adaptations of glutathione content in cucurbita pepo l. induced by treatment with reduced glutathione and buthionine sulfoximine. | the intracellular effects of gsh (reduced glutathione) and bso (buthionine sulfoximine) treatment on glutathione content were investigated with immunogold labeling in individual cellular compartments of cucurbita pepo l. seedlings. generally, gsh treatment led to increased levels of glutathione in roots and leaves (up to 3.5-fold in nuclei), whereas bso treatment significantly decreased glutathione content in all organs. transmission electron microscopy revealed that glutathione levels in mitoch ... | 2006 | 16520878 |
| growth conditions impact 2,2-bis(p-chlorophenyl)-1,1-dichloroethylene (p,p'-dde) accumulation by cucurbita pepo. | laboratory experiments were conducted to study the effects of soil moisture content, planting density, plant age, and the growth of multiple generations on the bioconcentration of weathered p,p'-dde by the plant cucurbita pepo. as soil moisture content increased from 7.4% to 29.9% (by weight), rates of contaminant accumulation by plant roots were increased by more than a factor of 2. higher planting density also led to higher uptake, as the root bioconcentration factor (bcf, dry-weight ratio of ... | 2006 | 17120529 |
| immunocytochemical localization of glutathione precursors in plant cells. | in plant cells, the antioxidant glutathione is synthesized out of its constituents cysteine, glutamate and glycine. as information about their subcellular and cellular distribution was not available in the past, the aim of the present study was to develop a technique that would allow visualization of their distribution in plant cells. with selective antibodies against cysteine, glutamate and glycine, it was possible, for the first time, to study the relative distribution of these components in m ... | 2006 | 16861702 |
| effect of mycorrhizal fungi on the phytoextraction of weathered p,p-dde by cucurbita pepo. | field experiments were conducted to assess the impact of inoculation with mycorrhizal fungi on the accumulation of weathered p,p'-dde from soil by three cultivars of zucchini (cucurbita pepo spp. pepo cv costata romanesco, goldrush, raven). three commercially available mycorrhizal products (biovam, myco-vam, invam) were inoculated into the root system of the zucchini seedlings at planting. in agreement with our previous findings, plants not inoculated with fungi accumulated large but variable am ... | 2006 | 16777321 |
| first known image of cucurbita in europe, 1503-1508. | the genus cucurbita (pumpkin, squash, gourd) is native to the americas and diffused to other continents subsequent to the european contact in 1492. for many years, the earliest images of this genus in europe that were known to cucurbit specialists were the two illustrations of c. pepo pumpkins that were published in fuchs' de historia stirpium, 1542. images of fruits of two cucurbita species, drawn between 1515 and 1518, were recently discovered in the villa farnesina in rome. | 2006 | 16687431 |
| in vivo monitoring of chlorophyll fluorescence response to low-dose gamma-irradiation in pumpkin (cucurbita pepo) leaves. | the effect of gamma-irradiation on the in vivo-measured chlorophyll fluorescence in a pumpkin leaves (cucurbita pepo) has been investigated. plants were grown in the same environment, then divided into several groups and irradiated at ambient conditions at small dose levels (up to 13.4 gy) with (60)co gamma-rays. the post-irradiation effect on chlorophyll status in the leaves was examined by measuring chlorophyll fluorescence 2 days (48 h) after exposure. it is undoubtedly found that the value o ... | 2006 | 14587079 |
| extracts from pygeum africanum and other ethnobotanical species with antiandrogenic activity. | extracts from pygeum africanum, serenoa repens and cucurbita pepo are used in the treatment of benign prostatic hyperplasia (bph) and prostate cancer (pca). the activity of the androgen receptor (ar) is known to control growth of the prostate. here, we examined extracts of these plants for their antiandrogenic activity using an ar responsive reporter gene assay for drug discovery. a selective dichloromethane extract from the stem barks of pygeum africanum revealed the highest antiandrogenic effe ... | 2006 | 16783690 |
| in vitro antioxidative activity of pumpkin seed (cucurbita pepo) protein isolate and its in vivo effect on alanine transaminase and aspartate transaminase in acetaminophen-induced liver injury in low protein fed rats. | the antioxidative effects of pumpkin seed protein isolate (cucurbita pepo) were investigated in vitro. the isolate exhibited about 80% radical scavenging activity, chelating activity of approximately 64% on fe2+ ions and an inhibition of approximately 10% of xanthine oxidase. subsequently the effects of the isolate on the plasma activity levels of alanine transaminase and aspartate transaminase against acetaminophen induced acute liver injury in low-protein fed male sprague-dawley rats were asce ... | 2006 | 16807884 |
| inhibition of testosterone-induced hyperplasia of the prostate of sprague-dawley rats by pumpkin seed oil. | the oil from the pumpkin (cucurbita pepo) seed is claimed to be useful in the management of benign prostatic hyperplasia. this investigation seeks to examine the effect of pumpkin seed oil on testosterone-induced hyperplasia of the prostate of rats. hyperplasia was induced by subcutaneous administration of testosterone (0.3 mg/100 g of body weight) for 20 days. simultaneous oral administration of either pumpkin seed oil (2.0 and 4.0 mg/100 g of body weight) or corn oil (vehicle) was also given f ... | 2006 | 16822218 |
| compensatory capsid protein mutations in cucumber mosaic virus confer systemic infectivity in squash (cucurbita pepo). | cucumber mosaic virus (cmv) systemically infects both tobacco and zucchini squash. cmv capsid protein loop mutants with single-amino-acid substitutions are unable to systemically infect squash, but they revert to a wild-type phenotype in the presence of an additional, specific single-site substitution. the d118a, t120a, d192a, and d197a loop mutants reverted to a wild-type phenotype but did so in combination with p56s, p77l, a162v, and i53f or t124i mutations, respectively. the possible effect o ... | 2006 | 16840352 |
| eastern north america as an independent center of plant domestication. | the status of eastern north america as an independent center of plant domestication has recently been called into question by a number of genetic and archaeological studies, which suggest that the region may not have witnessed the independent domestication of local crop plants, but rather may have been on the receiving end of domesticated crop plants introduced from mexico. here, i provide a synthesis of the currently available archaeological and genetic evidence from both eastern north america ... | 2006 | 16894156 |
| antioxidative effects of pumpkin seed (cucurbita pepo) protein isolate in ccl4-induced liver injury in low-protein fed rats. | the effects of pumpkin seed (cucurbita pepo) protein isolate on the plasma activity levels of catalase (ca), superoxide dismutase (sod), glutathione peroxidase (gshpx) and total antioxidant capacity (tac) as well as glucose-6-phosphatase (g6pase) in liver homogenates and lipid peroxidation (lpo-malondialdehyde-mda) levels in liver homogenates and liver microsomal fractions against carbon tetrachloride (ccl(4))-induced acute liver injury in low-protein fed sprague-dawley rats (rattus norvegicus) ... | 2006 | 16909447 |
| fatty acids by high-performance liquid chromatography and evaporative light-scattering detector. | a high-performance liquid chromatographic (hplc) separation method with an evaporative light-scattering detector (elsd) has been developed for the separation and quantitative analysis of fatty acid methyl esters (fame) in three different oils. reverse-phased c18 hplc separation of 13 fame is achieved using a methanol/water eluent mixture. the retention times (rt) reflect the elution behavior of these compounds on c18 reversed-phase hplc. the proposed method is tested on: soybean oil (glycine max ... | 2006 | 17007865 |
| potential for phytoremediation of polychlorinated biphenyl-(pcb-)contaminated soil. | weathered soils contaminated with commercial-grade aroclor 1260 from three sites in canada were used to investigate the polychlorinated biphenyl (pcb) phytoextraction potential of nine plant species (festuca arundinacea, glycine max, medicago sativa, phalaris arundinacea, lolium multiflorum, carex normalis, and three varieties of cucurbita pepo ssp. pepo) under controlled greenhouse conditions. the soils used varied in pcb concentration (90-4200 microg/g) and total organic content (0.06-2.02%). ... | 2006 | 17120525 |
| detection by rapd-pcr of polymorphism in populations of bemisia tabaci (genn.) collected on four host plants from iran. | the sweetpotato whitefly, bernisia tabaci (genn.) (hom: aleyrodidae) is a major pest of field crops, vegetables and ornamentals in iran. in this study, the infested leaves of cucumber (cucurnis sativus l.) zucchini (cucurbita pepo l.) eggplant (solanum melongena l.) and cotton (gossypium hirsutum l.) with whitefly nymphs and pupae were collected from iran, and were transferred to the laboratory. the newly emerged males and females of each population were released separately into a large cage set ... | 2006 | 17385530 |
| inbreeding effects on blossom volatiles in cucurbita pepo subsp. texana (cucurbitaceae). | self-pollination by plants gives rise to inbreeding depression. there is increasing recognition that plant inbreeding can have significant implications for interactions between plants and other organisms, including insects and pathogens. many of these interactions are mediated by plant-derived volatiles, but the effects of inbreeding on volatile production have not previously been investigated. we examined variation in flower volatile production by the wild gourd cucurbita pepo subsp. texana as ... | 2006 | 21642122 |
| influence of citric acid amendments on the availability of weathered pcbs to plant and earthworm species. | a series of small and large pot trials were conducted to assess the phytoextraction potential of several plant species for weathered polychlorinated biphenyls (pcbs) in soil (105 microg/g arochlor 1268). in addition, the effect of citric acid on pcb bioavailability to both plants and earthworms was assessed. under small pot conditions (one plant, 400 g soil), three cucurbits (cucurbita pepo ssp pepo [zucchini] and ssp ovifera [nonzucchini summer squash], cucumis sativus, cucumber) accumulated up ... | 2006 | 16615308 |
| accumulation of weathered polycyclic aromatic hydrocarbons (pahs) by plant and earthworm species. | experiments were conducted to assess the bioavailability of polyclycic aromatic hydrocarbons (pahs) in soil from a manufactured gas plant site. three plant species were cultivated for four consecutive growing cycles (28 days each) in soil contaminated with 36.3 microg/g total pah. during the first growth period, cucurbita pepo ssp. pepo (zucchini) tissues contained significantly greater quantities of pahs than did cucumis sativus (cucumber) and cucurbita pepo ssp. ovifera (squash). during the fi ... | 2006 | 16337258 |
| molecular cloning and characterization of patellin1, a novel sec14-related protein, from zucchini (cucurbita pepo). | a full-length patellin1 (patl1) cdna was cloned and characterized from zucchini (cucurbita pepo). patl1, originally discovered in the higher plant arabidopsis thaliana, is a plant sec14-related protein that localizes to the cell plate during the late stages of cytokinesis. patl1 is related in sequence to other eukaryotic proteins involved in membrane trafficking and is thought to participate in vesicle trafficking events associated with cell plate maturation. the zucchini patl1 (cppatl1) cdna pr ... | 2006 | 16542754 |
| [relationships between host preference of liriomyza huidobrensis (blanchard) and nutrient and chlorophyll contents in host foliage]. | the laboratory study with no free choice means showed that the host preference of liriomyza huidobrensis (blanchard) adult differed significantly with test plants. phaseolus vulgaris, chrysanthemum coronarium and cucurbita pepo were the most preferred, while lycopersicum esculenturn and brassica oleracea were the least. correlative analysis indicated that the host preference of l. huidobrensis adult had a significant positive correlation with the content of soluble sugar, but less correlation wi ... | 2007 | 17552217 |
| assessment of attractiveness of plants as roosting sites for the melon fly, bactrocera cucurbitae, and oriental fruit fly, bactrocera dorsalis. | the use of toxic protein bait sprays to suppress melon fly, bactrocera cucurbitae (coquillett) (diptera: tephritidae), populations typically involves application to vegetation bordering agricultural host areas where the adults seek shelter ("roost"). although bait spray applications for suppression of oriental fruit fly, bactrocera dorsalis (hendel), populations have traditionally been applied to the host crop, rather than to crop borders, roosting by oriental fruit flies in borders of some crop ... | 2007 | 20334596 |
| phytoextraction of excess soil phosphorus. | in the search for a suitable plant to be used in p phytoremediation, several species belonging to legume, vegetable and herb crops were grown in p-enriched soils, and screened for p accumulation potentials. a large variation in p concentrations of different plant species was observed. some vegetable species such as cucumber (cucumis sativus) and yellow squash (cucurbita pepo var. melopepo) were identified as potential p accumulators with >1% (dry weight) p in their shoots. these plants also disp ... | 2007 | 16904249 |
| uptake and translocation of p,p'-dichlorodiphenyldichloroethylene supplied in hydroponics solution to cucurbita. | field studies show shoots of zucchini (cucurbita pepo l.) accumulate various hydrophobic contaminants from soil, although many other plants do not, including cucumber (cucumis sativus l.). to investigate the mechanism for this uptake, we presented p,p'-dichlorodiphenyldichloroethylene (dde) to these two species in hydroponics solution. a mixture of dde bound to tenax beads stirred with a solution of water passing through a reservoir provided a flowing solution containing dde at approximately 2 m ... | 2007 | 18020671 |
| differences in p,p'-dde bioaccumulation from compost and soil by the plants cucurbita pepo and cucurbita maxima and the earthworms eisenia fetida and lumbricus terrestris. | two plant species, cucurbita pepo and cucurbita maxima, and two earthworm species, eisenia fetida and lumbricus terrestris, were exposed to soil and compost with equivalent p,p'-dde contamination. pollutant bioconcentration was equal in plant roots in both media, but translocation was higher in c. pepo. bioaccumulation by e. fetida was approximately 6- and 3-fold higher than that by l. terrestris in the soil and compost, respectively. for all species, p,p'-dde uptake was significantly greater fr ... | 2007 | 17241722 |
| qualitative and quantitative differences in carotenoid composition among cucurbita moschata, cucurbita maxima, and cucurbita pepo. | squashes and pumpkins are important dietary sources of carotenoids worldwide. the carotenoid composition has been determined, but reported data have been highly variable, both qualitatively and quantitatively. in the present work, the carotenoid composition of squashes and pumpkins currently marketed in campinas, brazil, were determined by hplc-dad, complemented by hplc-ms for identification. cucurbita moschata 'menina brasileira' and c. moschata 'goianinha' had similar profiles, with beta-carot ... | 2007 | 17444652 |
| protein inhibitors of trypsin from the seeds of cucurbitaceae plants. | seven trypsin inhibitors were isolated from the seeds of cucurbitaceae plants: two from cucumber (cucumis sativus) and red bryony (bryonia diotica) and one from figleaf gourd (cucurbita ficifolia), spaghetti squash (cucurbita pepo var. (vegetable spaghetti) and water melon (citrullus vulgaris). the inhibitors were purified by fractionation with ammonium sulphate, followed by ion-exchange chromatography and affinity chromatography using immobilized trypsin or anhydro-trypsin. the homogeneous inhi ... | 2007 | 3450102 |
| identification and characterization of a novel whitefly-transmitted member of the family potyviridae isolated from cucurbits in florida. | abstract a novel whitefly-transmitted member of the family potyviridae was isolated from a squash plant (cucurbita pepo) with vein yellowing symptoms in florida. the virus, for which the name squash vein yellowing virus (sqvyv) is proposed, has flexuous rod-shaped particles of approximately 840 nm in length. the experimental host range was limited to species in the family cucurbitaceae, with the most dramatic symptoms observed in squash and watermelon, but excluded all tested species in the fami ... | 2007 | 18944369 |
| zucchini yellow mosaic virus: insect transmission and pathogenicity -the tails of two proteins. | summary taxonomy: zucchini yellow mosaic virus (zymv) is a member of genus potyvirus, family potyviridae. zymv is a positive-strand rna virus. physical properties: virions are flexuous filaments of 680-730 nm in length and 11-13 nm in diameter, composed of about 2000 subunits of a single 31-kda protein (calculated). the genome rna size is 9.6 kb covalently linked to a viral-encoded protein (the vpg) at the 5' end, and with a 3' poly a tail. the 5' end of the sequence is au-rich (69%). viral prot ... | 2007 | 20507486 |
| physiological and metabolic changes of cucurbita pepo leaves in response to zucchini yellow mosaic virus (zymv) infection and salicylic acid treatments. | the changes of some physiological and biochemical parameters in pumpkin (cucurbita pepo cv eskandarani) leaves associated with zucchini yellow mosaic virus (zymv) infection and the effect of exogenous application of salicylic acid (sa) were studied in this paper. in comparison to the untreated leaves, zymv infected leaves showed many symptoms, including severe mosaic, size reduction, stunting and deformation. results from analysis of physiological parameters indicated that viral infection and sa ... | 2007 | 17466528 |
| ethnoveterinary medicines used to treat endoparasites and stomach problems in pigs and pets in british columbia, canada. | this paper documents the medicinal plants used to treat endoparasites and stomach problems in dogs, cats and pigs in british columbia, canada. ethnoveterinary data was collected over a 6-month period in 2003. the majority of the information on pets came from 2 naturopaths, 10 herbalists, 5 dog trainers, breeders and pet shop owners, 9 holistic veterinarians and 6 of 27 organic farmers. two pig farmers joined the study in the final stages. the following plants were used as anthelmintics: artemisi ... | 2007 | 17628343 |
| content of nutrient and antinutrient in edible flowers of wild plants in mexico. | nutrient and antinutritional/toxic factors present in some edible flowers consumed in mexico were determined. the edible flowers were: agave salmiana, aloe vera, arbutus xalapensis, cucurbita pepo (cultivated), erythrina americana, erythrina caribaea, euphorbia radians benth and yucca filifera. the nutrient content in the flowers studied is similar to that of the edible leaves and flowers studied mainly in africa. the moisture content of the flowers varied from 860 to 932 g kg(-1). crude protein ... | 2007 | 17768684 |
| artificial elevation of glutathione affects symptom development in zymv-infected cucurbita pepo l. plants. | styrian oil pumpkin seedlings (cucurbita pepo l. subsp. pepo var. styriaca greb: .) were treated for 48 h with 1 mm otc (l-2-oxothiazolidine-4-carboxylic acid) in order to artificially increase cellular glutathione content. they were inoculated with zucchini yellow mosaic virus (zymv) 10 days later. the effects of otc treatment and zymv infection on glutathione levels were examined at the subcellular level by immunogold labeling of glutathione using a transmission electron microscope (tem). thes ... | 2007 | 17143780 |
| virus-induced changes in the subcellular distribution of glutathione precursors in cucurbita pepo (l.). | changes in glutathione contents occur in plants during environmental stress situations, such as pathogen attack, as the formation of reactive oxygen species leads to the activation of the antioxidative defence system. as glutathione is synthesized out of its constituents cysteine, glycine, and glutamate the availability of these components will limit glutathione synthesis in plants especially during stress situations and therefore the ability of the plant to fight oxidative stress. to gain a dee ... | 2007 | 17143806 |
| membrane associated qualitative differences in cell ultrastructure of chemically and high pressure cryofixed plant cells. | membrane contrast can sometimes be poor in biological samples after high pressure freezing (hpf) and freeze substitution (fs). the addition of water to the fs-medium has been shown to improve membrane contrast in animal tissue and yeast. in the present study we tested the effects of 1% and 5% water added to the fs-medium (2% osmium with 0.2% uranyl acetate in anhydrous acetone) on the quality and visibility of membranes in high pressure frozen leaf samples of cucurbita pepo l. plants and compare ... | 2007 | 17270463 |
| ethnomedicines used in trinidad and tobago for reproductive problems. | throughout history women have tried to control or enhance their fertility using herbal remedies, with various levels of societal support. caribbean folk medicine has been influenced by european folk medicine, either through the early spanish and french settlers or through the continuous immigration of spanish-speaking peoples from venezuela. some folk uses are ancient and were documented by galen and pliny the elder. | 2007 | 17362507 |
| chlorophyllase is a rate-limiting enzyme in chlorophyll catabolism and is posttranslationally regulated. | chlorophyll is a central player in harvesting light energy for photosynthesis, yet the rate-limiting steps of chlorophyll catabolism and the regulation of the catabolic enzymes remain unresolved. to study the role and regulation of chlorophyllase (chlase), the first enzyme of the chlorophyll catabolic pathway, we expressed precursor and mature versions of citrus (citrus sinensis) chlase in two heterologous plant systems: (1) squash (cucurbita pepo) plants using a viral vector expression system; ... | 2007 | 17369368 |
| surfactants differentially impact p,p'-dde accumulation by plant and earthworm species. | the effect of four surfactants (triton x-100, tween-80, rhamnolipids, cyclodextrin) at 100-1000 mg/l on p,p'-dde phytoextraction by cucurbita pepo (zucchini) under field conditions and p,p'-dde bioaccumulation by earthworm species (eisenia fetida, lumbricus terrestris) under laboratory conditions was investigated. abiotically, surfactants (except cyclodextrin) increased contaminant desorption from soil by 4-fold, with higher concentrations generally promoting greater release. cyclodextrin had no ... | 2007 | 17533859 |
| ultrasonic treatment stimulates multiple shoot regeneration and explant enlargement in recalcitrant squash cotyledon explants in vitro. | ultrasonic treatment (0.5-2 min) stimulated multiple shoot regeneration to high levels in vitro from recalcitrant cotyledon explants of commercial squash (cucurbita pepo l.) cultivars ma'yan and bareqet, on murashige and skoog [physiol plant 15:473-497, 1962] (regeneration) medium augmented with 4.4 microm benzyladenine. at this stage, unsonicated control explants regenerated only a few very small shoots or bud-like structures. ultrasound also stimulated massive explant growth. ultrasound treatm ... | 2007 | 17021848 |
| methyl jasmonate is a more effective senescence-promoting factor in cucurbita pepo (zucchini) cotyledons when compared with darkness at the early stage of senescence. | the effects of short-term darkening and methyl jasmonate (meja) on cotyledon senescence were studied 24h after transfer of intact 7-day-old cucurbita pepo (zucchini) seedlings to darkness or spraying with 100 microm meja. the jasmonate inhibitory effect on chlorophyll content and chloroplast transcriptional activity was stronger compared with darkness. further, meja reduced the photosynthetic rate whereas darkness did not affect photosynthesis. neither stress factor affected the photochemical qu ... | 2007 | 16987568 |
| nectar resorption and translocation in cucurbita pepo l. and platanthera chlorantha custer (rchb.). | nectar resorption and sugar translocation were studied in cucurbita pepo (cucurbitaceae) and platanthera chlorantha (orchidaceae) by micro-autoradiography. in both species, nectar was resorbed in pollinated and unpollinated flowers and ovules developing into seeds were found to be the main sugar sink. in c. pepo, the mobility of resorbed sugars in pollinated female flowers was higher than in unpollinated ones; male flowers showed lower mobility of resorbed sugar. in p. chlorantha, radioactivity ... | 2007 | 16883483 |
| ascorbic acid oxidase is dynamically regulated by light and oxygen. a tool for oxygen management in plants? | ascorbic acid oxidase (aao) is a plant blue-copper protein catalyzing dioxygen reduction to water using ascorbic acid as the electron donor. in spite of extensive molecular characterization the physiological role of aao is still uncertain. abundant mrna, protein and activity of aao were observed in illuminated leaves of cucurbita pepo. aao activity was found to be proportional to light intensity. the light effect was rapidly reversed in dark and activity remained low throughout the dark period. ... | 2007 | 16343690 |