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host range and complete genome sequence of cucurbit chlorotic yellows virus, a new member of the genus crinivirus.cucurbit chlorotic yellows virus (ccyv) causes chlorotic yellows on cucumber (cucumis sativus) and melon (cucumis melo) and is transmitted by bemisia tabaci biotype b and q whiteflies. to characterize the host range of ccyv, 21 cucurbitaceous and 12 other plant species were inoculated using whitefly vectors. all tested cucumis spp. except cucumis anguria and cucumis zeyheri were systemically infected with ccyv, although infection rates varied among species. citrullus lanatus, cucurbita pepo, and ...201020465411
zucchini yellow mosaic virus: insect transmission and pathogenicity -the tails of two proteins.summary taxonomy: zucchini yellow mosaic virus (zymv) is a member of genus potyvirus, family potyviridae. zymv is a positive-strand rna virus. physical properties: virions are flexuous filaments of 680-730 nm in length and 11-13 nm in diameter, composed of about 2000 subunits of a single 31-kda protein (calculated). the genome rna size is 9.6 kb covalently linked to a viral-encoded protein (the vpg) at the 5' end, and with a 3' poly a tail. the 5' end of the sequence is au-rich (69%). viral prot ...200720507486
detection of plant dna in the bronchoalveolar lavage of patients with ventilator-associated pneumonia.hospital-acquired infections such as nosocomial pneumonia are a serious cause of mortality for hospitalized patients, especially for those admitted to intensive care units (icus). despite the number of the studies reported to date, the causative agents of pneumonia are not completely known. herein, we found by molecular technique that vegetable and tobacco dna may be detected in the bronchoalveolar lavage from patients with ventilator-associated pneumonia (vap).201020585574
rapid detection of squash leaf curl virus by loop-mediated isothermal amplification.a loop-mediated isothermal amplification (lamp) assay was employed to develop a simple and efficient system for the detection of squash leaf curl virus (slcv) in diseased plants of squash (cucurbita pepo) and melon (cucumis melo). completion of lamp assay required 30-60 min under isothermal conditions at 65 degrees c by employing a set of four primers targeting slcv. although the sensitivity of the lamp assay and the polymerase chain reaction (pcr) assay was comparable at high virus concentratio ...201020603151
protective role of three vegetable peels in alloxan induced diabetes mellitus in male mice.the hitherto unknown glucose regulating role of three vegetable peels from cucurbitaceae family was evaluated. in a preliminary study, effects of ethanolic extracts of cucurbita pepo, cucumis sativus and praecitrullus fistulosus peels were studied at 250 and 500 mg kg(-1) d(-1) for 15 days in the alterations in serum glucose and in hepatic lipid peroxidation (lpo) in male mice. in the pilot experiment, the effective and safe concentration of each peel was administered (p.o.) for 10 consecutive d ...201020614191
cytotoxicity of hydro-alcoholic extracts of cucurbitapepo and solanum nigrum on hepg2 and ct26 cancer cell lines.plants are used worldwide for the treatment of diseases, and novel drugs continue to be developed through research from plants. there are more than 20,000 species of plants used in traditional medicines, and these are all potential reservoirs for new drugs. cucurbitapepo has been used in traditional folk medicine to treat cold and alleviate ache. previous pharmacological tests have shown that it possesses antiviral, anti-inflammatory, and analgesic effects. also, solanum nigrum has been used as ...201020931075
suppressive effect of pectic polysaccharides from cucurbita pepo l. var. styriaca on citric acid-induced cough reflex in guinea pigs.several water-soluble pectic polysaccharides were isolated from the pumpkin fruit biomass and characterized by composition, structural features and molecular properties. the pectic polysaccharides were tested for antitussive activity by studying the effects of citric acid-induced cough reflex in guinea pigs and reactivity of the airway smooth muscle in vivo conditions in comparison to the narcotic drug codeine. oral administration of all pectic polysaccharides from pumpkin inhibited the number o ...201021062638
influence of preceding crop on wireworm (coleoptera: elateridae) abundance in the coastal plain of north carolina.three studies were conducted to determine the effect of preceding crop on wireworm (coleoptera: elateridae) abundance in the coastal plain of north carolina. in all three studies, samples of wireworm populations were taken from the soil by using oat, avena sativa l., baits. treatments were defined by the previous year's crop and were chosen to reflect common crop rotations in the region. across all three studies, eight wireworm species were recovered from the baits: conoderus amplicollis (gyllen ...201021309229
effects of cucumber mosaic virus infection on vector and non-vector herbivores of squash.plant chemicals mediating interactions with insect herbivores seem a likely target for manipulation by insectvectored plant pathogens. yet, little is currently known about the chemical ecology of insect-vectored diseases or their effects on the ecology of vector and nonvector insects. we recently reported that a widespread plant pathogen, cucumber mosaic virus (cmv), greatly reduces the quality of host-plants (squash) for aphid vectors, but that aphids are nevertheless attracted to the odors of ...201021331245
watermelon chlorotic stunt virus (wmcsv): a serious disease threatening watermelon production in jordan.the incidence of watermelon chlorotic stunt disease and the molecular characterization of the jordanian isolate of watermelon chlorotic stunt virus (wmcsv-[jo]) are described in this study. symptomatic leaf samples obtained from watermelon (citrullus lanatus thunb.), melon (cucumis melo l.), squash (cucurbita pepo), cucumber (cucumis sativus l.), and bottle gourd (lagenaria siceraria) plants were tested for wmcsv-[jo] infection by pcr. the virus could be detected in 8 melon and 87 watermelon sam ...201121399920
occurrence of clostridium perfringens from different cultivated soils.the occurrence of clostridium perfringens was estimated in 750 samples originated from a variety of soils bearing various bulb crops: brawnica oderacea (vegetable), olea europaea, daucus carota (carote), solanum tuberosum (potato), phaseolus vulgaris (green haricot), beta vulgaris var. rapaceum (beetroot), cucurbita pepo (squash), allium cepa (onion), cucumis sativus (cucumber) and capsicum annum (pepper). all isolated strains were tested for their antimicrobial activities to amoxicillin, penici ...201121621626
was the famous astronomer copernicus also a nephrologist.nicolaus copernicus (1473-1543), world-famous astronomer, born in toruń, was also a warmian canon (senior priest) and a physician to 4 consecutive prince-bishops of warmia and of other warmian canons. what medical conditions preoccupied nicolaus copernicus and whether they included kidney diseases can only be inferred from the extant prescriptions of copernicus, as no record remains of any treatises by copernicus regarding medicine. while no prescription penned by him is dated, several are trace ...201121614777
antimicrobial nectar inhibits a florally transmitted pathogen of a wild cucurbita pepo (cucurbitaceae).• premise of the study: floral nectars of many species contain antimicrobial chemicals, but their function in nectar is subject to debate. previously, we have shown that erwinia tracheiphila, the causative agent of bacterial wilt disease in cucurbits, can be transmitted via the floral nectaries. • methods: we used a disk diffusion assay (dda) to determine the antimicrobial effects of nectar from a wild gourd on lawns of escherichia coli and erwinia tracheiphila. we also used e. tracheiphila to i ...201021622472
inbreeding effects on blossom volatiles in cucurbita pepo subsp. texana (cucurbitaceae).self-pollination by plants gives rise to inbreeding depression. there is increasing recognition that plant inbreeding can have significant implications for interactions between plants and other organisms, including insects and pathogens. many of these interactions are mediated by plant-derived volatiles, but the effects of inbreeding on volatile production have not previously been investigated. we examined variation in flower volatile production by the wild gourd cucurbita pepo subsp. texana as ...200621642122
zucchini yellow mosaic virus: biological properties, detection procedures and comparison of coat protein gene sequences.between 2006 and 2010, 5324 samples from at least 34 weed, two cultivated legume and 11 native species were collected from three cucurbit-growing areas in tropical or subtropical western australia. two new alternative hosts of zucchini yellow mosaic virus (zymv) were identified, the australian native cucurbit cucumis maderaspatanus, and the naturalised legume species rhyncosia minima. low-level (0.7%) seed transmission of zymv was found in seedlings grown from seed collected from zucchini (cucur ...201121935626
Performance of Apis mellifera, Bombus impatiens, and Peponapis pruinosa (Hymenoptera: Apidae) as pollinators of pumpkin.Pollination services of pumpkin, Cucurbita pepo L., provided by the European honey bee, Apis mellifera L., were compared with two native bee species, the common eastern bumble bee, Bombus impatiens (Cresson), and Peponapis pruinosa Say, in New York from 2008 to 2010. Performance of each species was determined by comparing single-visit pollen deposition, percentage of visits that contacted the stigma, flower-handling time, fruit and seed set, and fruit weight per number of visits. Fruit yield fro ...201121882677
microbial diversity inside pumpkins: microhabitat-specific communities display a high antagonistic potential against phytopathogens.recent and substantial yield losses of styrian oil pumpkin (cucurbita pepo l. subsp. pepo var. styriaca greb.) are primarily caused by the ascomycetous fungus didymella bryoniae but bacterial pathogens are frequently involved as well. the diversity of endophytic microbial communities from seeds (spermosphere), roots (endorhiza), flowers (anthosphere), and fruits (carposphere) of three different pumpkin cultivars was studied to develop a biocontrol strategy. a multiphasic approach combining molec ...201121947430
flow injection assessment of nitrate contents in fresh and cooked fruits and vegetables grown in fiji.nitrates form part of the essential chemistry of soils and plants. thus, plant roots are able to absorb nitrate directly from the soil. it has been discovered that human nitrate intake is mainly from vegetables. vegetables play an important role in human nutrition since they are an outstanding source for vitamins, minerals, and biologically active compounds. in risk assessment of nitrate contents, this study reports the nitrate levels of 8 commonly consumed fruits and vegetables grown in fiji, t ...201122417577
combining ability of summer-squash lines with different degrees of parthenocarpy and prsv-w resistance.the aim was to assess heterosis in a set of 16 summer-squash hybrids, and evaluate the combining capacity of the respective parental lines, which differed as to the degree of parthenocarpy and resistance to prsv-w (papaya ringspot virus-watermelon strain). the hybrids were obtained using a partial diallel cross design (4 × 4). the lines of parental group i were 1 = abx-037g-77-03-05-01-01-bulk, 2 = abx-037g-77-03-05-03-10-bulk, 3 = abx-037g-77-03-05-01-04-bulk and 4 = abx-037g-77-03-05-05-01-bul ...201122215966
structural diversity of pectins isolated from the styrian oil-pumpkin (cucurbita pepo var. styriaca) fruit.to evaluate the seeded fruit biomass of the styrian oil-pumpkin in view of its pectin component, a series of acidic polysaccharides were isolated by a six-step sequential extraction using hot water, edta, dilute hcl (twice) and dilute and stronger naoh solutions. chemical, physicochemical and spectroscopy analyses revealed that the first four fractions comprised partially methyl-esterified and acetylated pectins with varying proportions of rhamnogalacturonan regions ramified with galactose- and ...201223465915
identification of the regulatory steps in gluconeogenesis in cotyledons of cucurbita pepo.1. the aim of this work was to discover the steps at which the conversion of oxaloacetate to glucose 6-phosphate during gluconeogenesis is regulated in the cotyledons of 5-day-old seedlings of cucurbita pepo. 2. we estimated the maximum catalytic activities of all the enzymes in the above sequence and also the amounts of their substrates present in vivo. the results show that the reactions catalysed by fructose-1,6-bisphosphatase and phosphoenolpyruvate carboxykinase are the only ones in the seq ...1990208645
ascorbate oxidase from cucurbita pepo medullosa. new method of purification and reinvestigation of properties.1. ascorbate oxidase has been isolated from the green squash cucurbita pepo medullosa by a new purification method. furthermore a low-molecular-weight copper protein containing one type-1 copper/20000 mr could be separated during the purification of the oxidase. the six-step procedure developed improved the yield of ascorbate oxidase by a factor of 2.5. the method is well reproducible and a constant value of 8 cu (7.95 +/- 0.1/140000 mr) has been established. by ultracentrifugal and electrophore ...1996228938
protein inhibitors of trypsin from the seeds of cucurbitaceae plants.seven trypsin inhibitors were isolated from the seeds of cucurbitaceae plants: two from cucumber (cucumis sativus) and red bryony (bryonia diotica) and one from figleaf gourd (cucurbita ficifolia), spaghetti squash (cucurbita pepo var. (vegetable spaghetti) and water melon (citrullus vulgaris). the inhibitors were purified by fractionation with ammonium sulphate, followed by ion-exchange chromatography and affinity chromatography using immobilized trypsin or anhydro-trypsin. the homogeneous inhi ...20073450102
infection of host plants by cucumber mosaic virus increases the susceptibility of myzus persicae aphids to the parasitoid aphidius colemani.plant viruses can profoundly alter the phenotypes of their host plants, with potentially far-reaching implications for ecology. yet few studies have explored the indirect, host-mediated, effects of plant viruses on non-vector insects. we examined how infection of cucurbita pepo plants by cucumber mosaic virus (cmv) impacted the susceptibility of aphids (myzus persicae) to attack by the parasitoid wasp aphidius colemani. in semi-natural foraging assays, we observed higher rates of aphid parasitis ...201526043237
composite cucurbita pepo plants with transgenic roots as a tool to study root development.in most plant species, initiation of lateral root primordia occurs above the elongation zone. however, in cucurbits and some other species, lateral root primordia initiation and development takes place in the apical meristem of the parental root. composite transgenic plants obtained by agrobacterium rhizogenes-mediated transformation are known as a suitable model to study root development. the aim of the present study was to establish this transformation technique for squash.201222553131
arbuscular mycorrhizal fungal hyphae alter soil bacterial community and enhance polychlorinated biphenyls dissipation.we investigated the role of arbuscular mycorrhizal fungal (amf) hyphae in alternation of soil microbial community and aroclor 1242 dissipation. a two-compartment rhizobox system with double nylon meshes in the central was employed to exclude the influence of cucurbita pepo l. root exudates on hyphal compartment soil. to assess the quantitative effect of amf hyphae on soil microbial community, we separated the hyphal compartment soil into four horizontal layers from the central mesh to outer wall ...201627379068
multicolor fluorescence imaging as a candidate for disease detection in plant phenotyping.the negative impact of conventional farming on environment and human health make improvements on farming management mandatory. imaging techniques are implemented in remote sensing for monitoring crop fields and plant phenotyping programs. the increasingly large size and complexity of the data obtained by these techniques, makes the implementation of powerful mathematical tools necessary in order to identify informative parameters and to apply them in precision agriculture. multicolor fluorescenc ...201627994607
effects of silicates from scaps of photovoltaic industries on powdery mildew of zucchini.silicon is the second most abundant element on earth's surface and its use can stimulate natural defense mechanisms in plants. the effect of silicate from scraps of photovoltaic industries against powdery mildew on zucchini (cucurbita pepo) was evaluated under greenhouse conditions. potted plants were inoculated with a spore suspension containing 1 x 10(5) cfu/ml. the following treatments have been carried out, 3 and 10 days after pathogen inoculation: chemical fungicide (propiconazole, tilt 25 ...201223878962
effects of solarization and ammonium amendments on plant-parasitic nematodes.the effects of soil solarization and ammonium bicarbonate or ammonium sulfate against plant-parasitic nematodes on yellow squash (cucurbita pepo) and on vinca (catharanthus roseus) were evaluated at two sites. solarization for 3 weeks in the spring suppressed population levels of belonolaimus longicaudatus, criconemella spp., and dolichodorus heterocephalus throughout the growing season on both crops at both sites. levels of meloidogyne incognita were suppressed initially, but population densiti ...200019271007
first report of cucumber mosaic virus isolated from wild vigna angularis var. nipponensis in korea.a viral disease causing severe mosaic, necrotic, and yellow symptoms on vigna angularis var. nipponensis was prevalent around suwon area in korea. the causal virus was characterized as cucumber mosaic virus (cmv) on the basis of biological and nucleotide sequence properties of rnas 1, 2 and 3 and named as cmv-wva. cmv-wva isolate caused mosaic symptoms on indicator plants, nicotiana tabacum cv. xanthi-nc, petunia hybrida, and cucumis sativus. strikingly, cmv-wva induced severe mosaic and malform ...201425289004
evidence of local adaptation in plant virus effects on host-vector interactions.recent research suggests that plant viruses, and other pathogens, frequently alter host-plant phenotypes in ways that facilitate transmission by arthropod vectors. however, many viruses infect multiple hosts, raising questions about whether these pathogens are capable of inducing transmission-facilitating phenotypes in phylogenetically divergent host plants and the extent to which evolutionary history with a given host or plant community influences such effects. to explore these issues, we worke ...201424748599
effects of virus on plant fecundity and population dynamics.microorganisms are ubiquitous and thought to regulate host populations. although microorganisms can be pathogenic and affect components of fitness, few studies have examined their effects on wild plant populations. as individual traits might not contribute equally to changes in population growth rate, it is essential to examine the entire life cycle to determine how microorganisms affect host population dynamics. in this study, we used data from common garden experiments with plants from three c ...201424571200
biochemical and physiological mechanisms underlying effects of cucumber mosaic virus on host-plant traits that mediate transmission by aphid vectors.the transmission of insect-vectored diseases entails complex interactions among pathogens, hosts and vectors. chemistry plays a key role in these interactions; yet, little work has addressed the chemical ecology of insect-vectored diseases, especially in plant pathosystems. recently, we documented effects of cucumber mosaic virus (cmv) on the phenotype of its host (cucurbita pepo) that influence plant-aphid interactions and appear conducive to the non-persistent transmission of this virus. cmv r ...201424329574
pepo aphid-borne yellows virus: a new species in the genus polerovirus.pepo aphid-borne yellows virus (pabyv) has been proposed as a putative representative of a new species in the genus polerovirus in the family luteoviridae. the genomes of two south african (sa) isolates of cucurbit-infecting pabyv were described in this record. total rna, extracted from a pattypan (cucurbita pepo l.) and a baby marrow (c. pepo l.) leaf samples, was subjected to next-generation sequencing (ngs) on the hiseq illumina platform. sanger sequencing was subsequently used to authenticat ...201727623838
draft genome sequence of erwinia tracheiphila, an economically important bacterial pathogen of cucurbits.erwinia tracheiphila is one of the most economically important pathogens of cucumbers, melons, squashes, pumpkins, and gourds in the northeastern and midwestern united states, yet its molecular pathology remains uninvestigated. here, we report the first draft genome sequence of an e. tracheiphila strain isolated from an infected wild gourd (cucurbita pepo subsp. texana) plant. the genome assembly consists of 7 contigs and includes a putative plasmid and at least 20 phage and prophage elements.201526044415
disease interactions in a shared host plant: effects of pre-existing viral infection on cucurbit plant defense responses and resistance to bacterial wilt disease.both biotic and abiotic stressors can elicit broad-spectrum plant resistance against subsequent pathogen challenges. however, we currently have little understanding of how such effects influence broader aspects of disease ecology and epidemiology in natural environments where plants interact with multiple antagonists simultaneously. in previous work, we have shown that healthy wild gourd plants (cucurbita pepo ssp. texana) contract a fatal bacterial wilt infection (caused by erwinia tracheiphila ...201324155951
pathogen effects on vegetative and floral odours mediate vector attraction and host exposure in a complex pathosystem.pathogens can alter host phenotypes in ways that influence interactions between hosts and other organisms, including insect disease vectors. such effects have implications for pathogen transmission, as well as host exposure to secondary pathogens, but are not well studied in natural systems, particularly for plant pathogens. here, we report that the beetle-transmitted bacterial pathogen erwinia tracheiphila - which causes a fatal wilt disease - alters the foliar and floral volatile emissions of ...201222988893
first report on fusarium wilt of zucchini caused by fusarium oxysporum, in korea.fusarium wilt of zucchini in jeonju, korea, was first noticed in may 2013. symptoms included wilting of the foliage, drying and withering of older leaves, and stunting of plants. infected plants eventually died during growth. based on morphological characteristics and phylogenetic analyses of the molecular markers (internal transcribed spacer rdna and translation elongation factor 1α), the fungus was identified as fusarium oxysporum. pathogenicity of a representative isolate was demonstrated via ...201526190927
draft genome sequence of enterobacter aerogenes, a dde-degrading and plant growth-promoting strain isolated from cucurbita pepo.we report here the draft genome of enterobacter aerogenes, a gram-negative bacterium of the enterobacteriaceae isolated from cucurbita pepo root tissue. this bacterium shows 2,2-bis(p-chlorophenyl)-1,1-dichloroethylene (dde)-degrading potential and plant growth-promoting capacity. an analysis of its 4.5-mb draft genome will enhance the understanding of dde degradation pathways and phytoremediation applications for dde-contaminated soils.201525883299
suitability of zucchini and cucumber genotypes to populations of meloidogyne arenaria, m. incognita, and m. javanica.the host suitability of five zucchini and three cucumber genotypes to meloidogyne incognita (mipm26) and m. javanica (mj05) was determined in pot experiments in a greenhouse. the number of egg masses (em) did not differ among the genotypes of zucchini or cucumber, but the eggs/plant and reproduction factor (rf) did slightly. m. incognita mipm26 showed lower em, eggs/plant, and rf than m. javanica mj05. examination of the zucchini galls for nematode postinfection development revealed unsuitable c ...201525861120
rate response of 1,3-dichloropropene for nematode control in spring squash in deep sand soils.the soil fumigant 1,3-dichloropropene (1,3-d) formulated with chloropicrin is viewed as a likely alternative for replacing methyl bromide in florida when the latter is phased out in 2005. therefore, it behooves us to learn more about using 1,3-d in deep, sand soils. two trials were conducted on spring squash to determine the most effective rate of 1,3-d for the control of meloidogyne spp. rates tested included 0, 56, 84, 112, and 168 liters/ha of 1,3-d applied broadcast with conventional chisels ...200019271005
responses of meloidogyne arenaria and m. incognita to green manures and supplemental urea in glasshouse culture.the recent loss of many effective nematicides has led to renewed interest in alternative methods of nematode management. greenhouse experiments were conducted to determine the effects of rapeseed and velvetbean green manures, and supplemental urea, on the root-knot nematodes meloidogyne arenaria and m. incognita. green manures were incorporated with m. arenaria-infested soil using rates totaling 200,300, and 400 mg n/kg soil. squash plants grown in this soil were evaluated using a gall index and ...199619277190
tropical rotation crops influence nematode densities and vegetable yields.the effects of eight summer rotation crops on nematode densities and yields of subsequent spring vegetable crops were determined in field studies conducted in north florida from 1991 to 1993. the crop sequence was as follows: (i) rotation crops during summer 1991; (ii) cover crop of rye (secale cereale) during winter 1991-92; (iii) 'lemondrop l' squash (cucurbita pepo) during spring 1992; (iv) rotation crops during summer 1992; (v) rye during winter 1992-93; (vi) 'classic' eggplant (solanum melo ...199419279897
effects of tropical rotation crops on meloidogyne arenaria population densities and vegetable yields in microplots.the effects of 12 summer crop rotation treatments on population densities of meloidogyne arenaria race 1 and on yields of subsequent spring vegetable crops were determined in microplots. the crop sequence was: (i) rotation crops during summer 1991 ; (ii) cover crop of rye (secale cereale) during winter 1991-92; (iii) squash (cucurbita pepo) during spring 1992; (iv) rotation crops during summer 1992; (v) rye during winter 1992-93; (vi) eggplant (solanum melongena) during spring 1993. the 12 rotat ...199419279880
efficacy of various application methods of fluensulfone for managing root-knot nematodes in vegetables.fluensulfone is a new nematicide in the flouroalkenyl chemical group. a field experiment was conducted in 2012 and 2013 to evaluate the efficacy of various application methods of fluensulfone for control of meloidogyne spp. in cucumber (cucumis sativus). treatments of fluensulfone (3.0 kg a.i./ha) were applied either as preplant incorporation (ppi) or via different drip irrigation methods: drip without pulse irrigation (drip np), pulse irrigation 1 hr after treatment (drip +1p), and treatment at ...201627418698
effect of crotalaria juncea amendment on squash infected with meloidogyne incognita.two greenhouse experiments were conducted to examine the effect of crotalaria juncea amendment on meloidogyne incognita population levels and growth of yellow squash (cucurbita pepo). in the first experiment, four soils with a long history of receiving yard waste compost (ywc+), no-yard-waste compost (ywc-), conventional tillage, or no-tillage treatments were used; in the second experiment, only one recently cultivated soil was used. half of the amount of each soil received air-dried residues of ...200419262819
effect of crotalaria juncea amendment on nematode communities in soil with different agricultural histories.effect of sunn hemp (crotalaria juncea) hay amendment on nematode community structure in the soil surrounding roots of yellow squash (cucurbita pepo) infected with root-knot nematodes was examined in two greenhouse experiments. soils were from field plots treated long-term (lt) with yard-waste compost or no yard-waste compost in lt experiment, and from a short-term (st) agricultural site in st experiment. soils collected were either amended or not amended with c. juncea hay. nematode communities ...200319262764
effects of a resistant corn hybrid and fenamiphos on meloidogyne incognita in a corn-squash rotation.the efficacy of a double-cross corn (zea mays) hybrid (old raccoon selection x t216) x (tebeau selection x mp 307) resistant to meloidogyne incognita as a rotational crop, and fenamiphos treatment for management of root-knot nematode (m. incognita race 1) in squash (cucurbita pepo var. melopepo) was evaluated in field tests during 1996 and 1997. numbers of m. incognita in the soil and root-gall indices were lower on the resistant hybrid than on a commercial cultivar dekalb dk-683. treatment mean ...199919270888
effects and carry-over benefits of nematicides in soil planted to a sweet corn-squash-vetch cropping system.the effects of irrigation on the efficacy of nematicides on meloidogyne incognita race 1 population densities, yield of sweet corn, and the carry-over of nematicidal effect in the squash crop were determined in a sweet corn-squash-vetch cropping system for 3 years. fenamiphos 15g and aldicarb 15g were applied at 6.7 kg a.i./ha and incorporated 15 cm deep with a tractor-mounted rototiller. ethylene dibromide (edb) was injected at 18 kg a.i./ha on each side of the sweet corn rows (total 36 kg a.i. ...199519277323
effect of simulated rainfall on leaching and efficacy of fenamiphos.there is increasing concern in the united states about the pesticide movement in soil, groundwater contamination, and pesticide residue in food. the objective of this study was to determine the efficacy, degradation, and movement of fenamiphos (nemacur 15g) in the soil and residues in squash fruit as influenced by four simulated rainfall treatments (2.5 or 5.0 cm each applied 1 or 3 days after nematicide application) under field conditions. in 1990, concentrations of fenamiphos were greater in t ...199519277322
effect of yard waste compost on plant-parasitic nematode densities in vegetable crops.the effects of yard-waste compost on densities of plant-parasitic nematodes were determined on four crops at two sites in north florida. separate experiments were conducted with sweet corn (zea mays), cowpea (vigna unguiculata), yellow squash (cucurbita pepo), and okra (hibiscus esculentus). in each test, the design was a randomized complete block replicated four times and involving three treatments: 269 mt/ha yard-waste compost applied to the soil surface as a mulch, 269 mt/ha compost incorpora ...199519277320
efficacy of fenamiphos formulations applied through irrigation for control of meloidogyne incognita on squash.management ofmeloidogyne incognita by chemigation with fenamiphos was studied in an infested field planted to m. incognita-suscepfible yellow summer squash cv. dixie hybrid. fenamiphos (vl 73.1% a.i. manufacturing concentrate in propylene glycol) was mixed with unitol dsr-90 or used as fenamiphos 3 sc (spray concentrate). both formulations, applied with 63.5 kl irrigation water per hectare, decreased numbers of m. incognita second-stage juveniles in the soil and root-gall indices, and increased ...199419279950
effects of rapeseed and vetch as green manure crops and fallow on nematodes and soil-borne pathogens.in a rapeseed-squash cropping system, meloidogyne incognita race 1 and m. javanica did not enter, feed, or reproduce in roots of seven rapeseed cultivars. both nematode species reproduced at low levels on roots of the third crop of rapeseed. reproduction of m. incognita and m. javanica was high on squash following rapeseed, hairy vetch, and fallow. the application of fenamiphos suppressed (p = 0.05) root-gall indices on squash following rapeseed, hairy vetch, and fallow; and on dwarf essex and c ...199219283212
management of root-knot nematodes by phenamiphos applied through an irrigation simulator with various amounts of water.phenamiphos (6.7 kg a.i./ha) was applied via an irrigation simulator to squash at planting (ap) and 2 weeks after planting (pp), and to corn ap and 1 week pp to manage root-knot nematodes (meloidogyne incognita). the nematicide was applied with 0.25, 0.64, 1.27, and 1.91 cm surface water/ ha to a lakeland sand in which the soil moisture was at or near field capacity. based on efficacy and crop response, no additional benefits resulted when phenamiphos was applied in volumes of water greater than ...198619294192
optimum concentrations of trichoderma longibrachiatum and cadusafos for controlling meloidogyne javanica on zucchini plants.a factorial experiment was established in a completely randomized design to verify the effect of different inoculum levels of an iranian isolate of trichoderma longibrachiatum separately and in combination with various concentrations of cadusafos against meloidogyne javanica in the greenhouse. zucchini seeds were soaked for 12 hr in five densities (0, 10(5), 10(6), 10(7), and 10(8) spores/ml suspension) of the fungus prior to planting in pots containing four concentrations of cadusafos (0, 0.5, ...201627168653
draft genome sequence of methylobacterium radiotolerans, a dde-degrading and plant growth-promoting strain isolated from cucurbita pepo.we announce the draft genome of methylobacterium radiotolerans, a gram-negative bacterium isolated from cucurbita pepo roots. this strain shows 2,2-bis(p-chlorophenyl)-1,1-dichloroethylene (dde)-degrading potential and plant growth-promoting capacities. analyses of its 6.8-mb genome will improve our understanding of dde-degradation pathways and aid in the deployment of phytoremediation technologies to remediate dde-contaminated soils.201525977414
genome sequence analysis of two south african isolates of moroccan watermelon mosaic virus infecting cucurbits.moroccan watermelon mosaic virus (mwmv) has been prevalent in cucurbits in the republic of south africa (rsa) since it was first reported in 1987. however, full genome studies of the south african isolates have never been conducted previously. the full genome of two mwmv isolates infecting cucurbits (cucurbita pepo l.) in the province of kwazulu-natal, rsa, was compared with the genome of the tunisian isolate in this communication. the genome sequences of the rsa mwmv isolates were elucidated us ...201627448681
zucchini shoestring virus: a distinct potyvirus in the papaya ringspot virus cluster.zucchini shoestring virus (zssv) has been proposed to be a putative potyvirus in the papaya ringspot virus (prsv) cluster, based on the sequence similarity of its coat protein to those of related potyviruses. zssv has been associated with the outbreak of a damaging disease of baby marrow (cucurbita pepo l.) that had been observed throughout the province of kwazulu-natal, in the republic of south africa (rsa). we report the genome sequence of zssv, determined by next-generation sequencing of tota ...201627216927
occurrence of squash yellow mild mottle virus and pepper golden mosaic virus in potential new hosts in costa rica.leaf samples of solanum lycopersicum, capsicum annuum, cucurbita moschata, cucurbita pepo, sechium edule and erythrina spp. were collected. all samples were positive for begomoviruses using polymerase chain reaction and degenerate primers. a sequence of ∼1,100 bp was obtained from the genomic component dna-a of 14 samples. in addition, one sequence of ∼580 bp corresponding to the coat protein (av1) was obtained from a chayote (s. edule) leaf sample. the presence of squash yellow mild mottle viru ...201325288955
pumpkin seed extract: cell growth inhibition of hyperplastic and cancer cells, independent of steroid hormone receptors.pumpkin seeds have been known in folk medicine as remedy for kidney, bladder and prostate disorders since centuries. nevertheless, pumpkin research provides insufficient data to back up traditional beliefs of ethnomedical practice. the bioactivity of a hydro-ethanolic extract of pumpkin seeds from the styrian pumpkin, cucurbita pepo l. subsp. pepo var. styriaca, was investigated. as pumpkin seed extracts are standardized to cucurbitin, this compound was also tested. transactivational activity wa ...201626976217
squash leaf curl virus (slcv): a serious disease threatening cucurbits production in palestine.the incidence of squash leaf curl disease and molecular characterization of the palestinian isolate of squash leaf curl virus [slcv-(pal)] are described in this study. symptomatic leaf samples obtained from squash (cucurbita pepo), watermelon [citrullus lanatus (thunb.)], and cucumber (cucumis sativus l.) plants were tested for slcv-[pal] infection by pcr and rca. slcv was also found to occur naturally in chenopodium murale, convolvulus sp, and prosporis farcta which showed yellowing. the diseas ...201424258392
effects of virus infection on pollen production and pollen performance: implications for the spread of resistance alleles.studies over the past 25 years have shown that environmental stresses adversely affect male function, including pollen production and pollen performance (germination and pollen tube growth rate). consequently, genetic variation among plants in resistance to a stress has the potential to impact pollen donation to conspecifics and, if deposited onto a stigma, the ability of the pollen to achieve fertilization. we examined the effects of a nonlethal virus epidemic on pollen production and pollen pe ...201626905087
establishment of a simple and rapid gene delivery system for cucurbits by using engineered of zucchini yellow mosaic virus.the infectious full-length cdna clone of zucchini yellow mosaic virus (zymv) isolate pa (pzymv-pa), which was isolated from pumpkin, was constructed by utilizing viral transcription and processing signals to produce infectious in vivo transcripts. simple rub-inoculation of plasmid dnas of pzymv-pa was successful to cause infection of zucchini plants (cucurbita pepo l.). we further engineered this infectious cdna clone of zymv as a viral vector for systemic expression of heterologous proteins in ...201626889118
partially resistant cucurbita pepo showed late onset of the zucchini yellow mosaic virus infection due to rapid activation of defense mechanisms as compared to susceptible cultivar.zucchini yellow mosaic virus (zymv) is an emerging viral pathogen in cucurbit-growing areas wordwide. infection causes significant yield losses in several species of the family cucurbitaceae. to identify proteins potentially involved with resistance toward infection by the severe zymv-h isolate, two cucurbita pepo cultivars (zelena susceptible and jaguar partially resistant) were analyzed using a two-dimensional gel electrophoresis-based proteomic approach. initial symptoms on leaves (clearing v ...201525972878
analysis of viral (zucchini yellow mosaic virus) genetic diversity during systemic movement through a cucurbita pepo vine.determining the extent and structure of intra-host genetic diversity and the magnitude and impact of population bottlenecks is central to understanding the mechanisms of viral evolution. to determine the nature of viral evolution following systemic movement through a plant, we performed deep sequencing of 23 leaves that grew sequentially along a single cucurbita pepo vine that was infected with zucchini yellow mosaic virus (zymv), and on a leaf that grew in on a side branch. strikingly, of 112 g ...201425107623
two and three dimensional characterization of zucchini yellow mosaic virus induced structural alterations in cucurbita pepo l. plants.infection of plants by zucchini yellow mosaic virus (zymv) induces severe ultrastructural changes. the aim of this study was to investigate ultrastructural changes during zymv-infection in cucurbita pepo l. plants on the two and three dimensional (2d and 3d) level and to correlate these changes with the spread of zymv throughout the plant by transmission electron microscopy (tem) and image analysis. this study revealed that after inoculation of the cotyledons zymv moved into roots [3 days post i ...201424631670
zucchini yellow mosaic virus (zymv, potyvirus): vertical transmission, seed infection and cryptic infections.the role played by seed transmission in the evolution and epidemiology of viral crop pathogens remains unclear. we determined the seed infection and vertical transmission rates of zucchini yellow mosaic virus (zymv), in addition to undertaking illumina sequencing of nine vertically transmitted zymv populations. we previously determined the seed-to-seedling transmission rate of zymv in cucurbita pepo ssp. texana (a wild gourd) to be 1.6%, and herein observed a similar rate (1.8%) in the subsequen ...201323845301
deep sequencing reveals persistence of intra- and inter-host genetic diversity in natural and greenhouse populations of zucchini yellow mosaic virus.the genetic diversity present in populations of rna viruses is likely to be strongly modulated by aspects of their life history, including mode of transmission. however, how transmission mode shapes patterns of intra- and inter-host genetic diversity, particularly when acting in combination with de novo mutation, population bottlenecks and the selection of advantageous mutations, is poorly understood. to address these issues, this study performed ultradeep sequencing of zucchini yellow mosaic vi ...201222592263
plant virus-induced changes in aphid population development and temporal fluctuations in plant nutrients.cucurbita pepo plants were infected with zucchini yellow mosaic virus or maintained noninfected.aphis gossypii, which transmits the virus, lived longer and produced more offspring on infected than on noninfected plants. on infected plants, the intrinsic rate of natural increase fora. gossypii increased with time after inoculation. in a similar experiment, concentrations of phloem sap nutrients, including free amino acids, total protein, and sugars from infected and noninfected plants were compar ...199424242121
alatae production and population increase of aphid vectors on virus-infected host plants.zucchini yellow mosaic virus (zymv) and aphis gossypii glover are two components of a recently identified plant-parasite system that provides an excellent opportunity to study interrelations between a virus and a vector that share the same host, but have no direct physiological interaction. in a field experiment we documented numbers of alate and apterous a. gossypii on healthy cucurbita pepo and on plants inoculated with virus 0, 7, 14, and 21 days before aphid infestation. when plants were ino ...199228311813
assessment of heavy metal pollution in vegetables and relationships with soil heavy metal distribution in zhejiang province, china.there are increasing concerns on heavy metal contaminant in soils and vegetables. in this study, we investigated heavy metal pollution in vegetables and the corresponding soils in the main vegetable production regions of zhejiang province, china. a total of 97 vegetable samples and 202 agricultural soil samples were analyzed for the concentrations of cd, pb, as, hg, and cr. the average levels of cd, pb, and cr in vegetable samples [chinese cabbage (brassica campestris spp. pekinensis), pakchoi ( ...201526013654
a rapid method for isolating glandular trichomes.a physical method is described for the rapid isolation of plant trichomes, with emphasis on stalked glandular types. the technique involved breaking frozen trichomes with powdered dry ice and collection of glandular heads by sieving from larger tissue fragments. this method was applied to several plants that bear similar stalked trichomes: geranium (pelargonium), potato (solanum tuberosum), tomato (lycopersicon esculentum), squash (cucurbita pepo), and velvetleaf (abutilon theophrasti). the tiss ...199216668834
curcurbita pepo subspecies delineates striped cucumber beetle (acalymma vittatum) preference.the striped cucumber beetle (acalymma vittatum (f.)) is a destructive pest of cucurbit crops, and management could be improved by host plant resistance, especially in organic farming systems. however, despite the variation in striped cucumber beetle preference observed within the economically important species, cucurbita pepo l., plant breeders and entomologists lacked a simple framework to classify and exploit these differences. this study used recent phylogenetic evidence and bioassays to orga ...201627347423
yield response of pumpkin and winter squash to simulated cucumber beetle (coleoptera: chrysomelidae) feeding injury.field studies were conducted to characterize the yield responses of pumpkin, cucurbita pepo l., and wintersquash, cucurbita maxima duch., to simulated striped cucumber beetle, acalymma vittatum (f.), feeding damage. preliminary studies in pumpkin ('spookie') were conducted in 1993, and more complete studies in 1994 and 1995 in pumpkin (spookie), and in 1995 and 1996 in winter squash ('waltham'). plants were artificially injured 1 time at the cotyledon, 1st, 2nd, or 3rd true leaf stages by clippi ...200014658523
companion planting with white yarrow or with feverfew for squash bug, anasa tristis (hemiptera: coreidae), management on summer squash.the squash bug, anasa tristis (de geer), is a major insect pest of cucurbits. control of squash bugs with insecticidal chemicals is difficult to achieve. we investigated the potential of companion planting with white yarrow (achillea millefolium l.) or feverfew [tanacetum parthenium (l.) sch. bip.] for squash bug management in field plantings of summer squash (cucurbita pepo l.).201627604891
the heterogeneity of phloem exudate proteins from different plants: a comparative survey of ten plants using polyacrylamide gel electrophoresis.proteins in sieve tube exudate from ricinus communis l., acer pseudoplatanus l., aesculus hippocastanum l., cucumis melo l., and two cultivars each of cucumis sativus l., cucurbita pepo l. and cucurbita maxima duchesne were fractionated and compared using polyacrylamide gel electrophoresis. striking differences in major exudate proteins were displayed among the genera and species examined. even cultivars within a single species, although showing general similarities, differed in some prominent p ...197624424985
de novo transcriptome assembly of two contrasting pumpkin cultivars.cucurbita pepo (squash, pumpkin, gourd), a worldwide-cultivated vegetable of american origin, is extremely variable in fruit characteristics. however, the information associated with genes and genetic markers for pumpkin is very limited. in order to identify new genes and to develop genetic markers, we performed a transcriptome analysis (rna-seq) of two contrasting pumpkin cultivars. leaves and female flowers of cultivars, 'big moose' with large round fruits and 'munchkin' with small round fruit ...201626981408
carbonaceous soil amendments to biofortify crop plants with zinc.carbonaceous soil amendments, comprising mixtures of biosolids and biochar, have been demonstrated to improve fertility while reducing nitrate leaching. we aimed to determine the efficacy of a biosolids/biochar soil amendment in biofortification of vegetables with zn, an element that is deficient in one third of humanity. we grew beetroot (beta vulgaris), spinach (spinacia oleracea), radish (raphanus sativus), broccoli (brassica oleracea), carrot (daucus carota), leek (allium ampeloprsum), onion ...201323146312
aphrodisiac activity of polyherbal formulation in experimental models on male rats.to investigate the aphrodisiac potential of polyherbal formulations prepared from different parts of tribulus terrestris, curculigo orchioides, allium tuberosum, cucurbita pepo, elephant creeper, mucuna pruriens, and terminalia catappa in albino rats in specified ratio as suspension.201424761115
electrophysiology of pumpkin seeds: memristors in vivo.leon chua, the discoverer of a memristor, theoretically predicted that voltage gated ion channels can be memristors. we recently found memristors in different plants such as the venus flytrap, mimosa pudica, aloe vera, apple fruits, and in potato tubers. there are no publications in literature about the existence of memristors in seeds. the goal of this work was to discover if pumpkin seeds might have memristors. we selected cucurbita pepo l., cv. cinderella, cucurbita maxima l. cv warty goblin, ...201626926652
[effects of host plants on the life table parameters of experimental populations of aphis gossypii].a comparative study was conducted on the life table parameters of aphis gossypii reared on five host plant species at (25 +/- 1) degrees c in laboratory. there existed significant differences in the durations of various developmental stages, adult longevity, mean offspring number per day, net reproductive rate, intrinsic rate of increase, finite rate of increase, mean generation time, and population doubling time for the a. gossypii populations reared on the host plants. for the aphids on lagena ...201324015567
effect of reproductive mode on host plant utilization of melon aphid (hemiptera: aphididae).variation in the reproductive mode of melon aphid aphis gossypii glover occurred on the large geographic scale, but the performance of different reproductive modes to use host plant is poorly understood. life tables of melon aphid population that undergo the anholocyclic, androcyclic, and intermediate reproductive mode were conducted on different host plants. the results showed that the anholocyclic and androcyclic strains could become adults and produce offspring on cotton gossypium hirsutum l. ...201022182553
predominant effects of host plant species on aphis gossypii aphid and e. pisyrphus balteatus hoverfly in a tritrophic approach.the aphid aphis gossypii glover (homoptera: aphididae) is known as worldwide important pest. it causes serious injuries to crops, but is also able to feed on various cultivated and wild plants. some programs are developped to control this aphid. in many studies, hoverfy episyrphus balteatus de geer (diptera: syrphidae) is used as an efficient and aphid specific predator in biological control systems. in this work, we study cotton aphid choice plant discrimination, the effect of host plants on it ...201122696952
the potential influence of bumble bee visitation on foraging behaviors and assemblages of honey bees on squash flowers in highland agricultural ecosystems.bee species interactions can benefit plant pollination through synergistic effects and complementary effects, or can be of detriment to plant pollination through competition effects by reducing visitation by effective pollinators. since specific bee interactions influence the foraging performance of bees on flowers, they also act as drivers to regulate the assemblage of flower visitors. we selected squash (cucurbita pepo l.) and its pollinators as a model system to study the foraging response of ...201626765140
use of video surveillance to measure the influences of habitat management and landscape composition on pollinator visitation and pollen deposition in pumpkin (cucurbita pepo) agroecosystems.pumpkin (cucurbita pepo) production relies on insect-mediated pollination, which is provided by managed and wild pollinators. the goals of this study were to measure the visitation frequency, longevity and temporal activity patterns of pumpkin pollinators and to determine if local habitat management and landscape composition affected this pollination service. we used video surveillance to monitor bee acitivty within male and female pumpkin flowers in 2011 and 2012 across a pollination window of ...201526587337
evaluating pollination deficits in pumpkin production in new york.potential decreases in crop yield from reductions in bee-mediated pollination services threaten food production demands of a growing population. many fruit and vegetable growers supplement their fields with bee colonies during crop bloom. the extent to which crop production requires supplementary pollination services beyond those provided by wild bees is not well documented. pumpkin, cucurbita pepo l., requires bee-mediated pollination for fruit development. previous research identified the comm ...201425198126
pollination services provided by bees in pumpkin fields supplemented with either apis mellifera or bombus impatiens or not supplemented.pollinators provide an important service in many crops. managed honey bees (apis mellifera l.) are used to supplement pollination services provided by wild bees with the assumption that they will enhance pollination, fruit set and crop yield beyond the levels provided by the wild bees. recent declines in managed honey bee populations have stimulated interest in finding alternative managed pollinators to service crops. in the eastern u.s., managed hives of the native common eastern bumble bee (bo ...201323894544
pollination value of male bees: the specialist bee peponapis pruinosa (apidae) at summer squash (cucurbita pepo).male bees can be abundant at flowers, particularly floral hosts of those bee species whose females are taxonomic pollen specialists (oligolecty). contributions of male bees to host pollination are rarely studied directly despite their prevalence in a number of pollination guilds, including those of some crop plants. in this study, males of the oligolectic bee, peponapis pruinosa say, were shown to be effective pollinators of summer squash, cucurbita pepo l. seven sequential visits from male p. p ...201122251639
wild bee abundance and pollination service in cultivated pumpkins: farm management, nesting behavior and landscape effects.recent declines in managed and feral honey bee populations have greatly increased interest in the current and potential role of wild pollinators in agricultural pollination. pumpkin (cucurbita pepo l.) has great potential to be served by wild pollinators because of a reliable and widespread group of bee species that are commonly associated with their flowers, including bumble bees (bombus spp.), and, in the americas, two genera of specialist ground nesting bees (peponapis and xenoglossa). we exa ...200919449636
brood pheromone regulates foraging activity of honey bees (hymenoptera: apidae).brood pheromone modulated the foraging behavior of commercial honey bee, apis mellifera l., colonies pollinating a 10-ha market garden of cucumber, cucurbita pepo l., and zucchini, cucumis saticus l., in texas in late autumn. six colonies were randomly selected to receive 2000 larval equivalents of brood pheromone and six received a blank control. the ratio of pollen to nonpollen foragers entering colonies was significantly greater in pheromone-treated colonies 1 h after treatment. pheromone-tre ...200415279247
molecular response of crop plants to engineered nanomaterials.functional toxicology has enabled the identification of genes involved in conferring tolerance and sensitivity to engineered nanomaterial (enm) exposure in the model plant arabidopsis thaliana (l.) heynh. several genes were found to be involved in metabolic functions, stress response, transport, protein synthesis, and dna repair. consequently, analysis of physiological parameters, metal content (through icp-ms quantification), and gene expression (by rt-qpcr) of a. thaliana orthologue genes were ...201627301997
association between photosynthesis and contrasting features of minor veins in leaves of summer annuals loading phloem via symplastic versus apoplastic routes.foliar vascular anatomy and photosynthesis were evaluated for a number of summer annual species that either load sugars into the phloem via a symplastic route (cucumis sativus l. cv. straight eight; cucurbita pepo l. cv. italian zucchini romanesco; citrullus lanatus l. cv. faerie hybrid; cucurbita pepo l. cv. autumn gold) or an apoplastic route (nicotiana tabacum l.; solanum lycopersicum l. cv. brandywine; gossypium hirsutum l.; helianthus annuus l. cv. soraya), as well as winter annual apoplast ...201424450755
leaf anatomical and photosynthetic acclimation to cool temperature and high light in two winter versus two summer annuals.acclimation of foliar features to cool temperature and high light was characterized in winter (spinacia oleracea l. cv. giant nobel; arabidopsis thaliana (l.) heynhold col-0 and ecotypes from sweden and italy) versus summer (helianthus annuus l. cv. soraya; cucurbita pepo l. cv. italian zucchini romanesco) annuals. significant relationships existed among leaf dry mass per area, photosynthesis, leaf thickness and palisade mesophyll thickness. while the acclimatory response of the summer annuals t ...201424450735
cloning and characterisation of a putative pollen-specific polygalacturonase gene (cppg1) differentially regulated during pollen development in zucchini (cucurbita pepo l.).studies in zucchini (cucurbita pepo l. spp. pepo) pollen have been limited to the viability and morphology of the mature pollen grain. the enzyme polygalacturonase (pg) is involved in pollen development and pollination in many species. in this work, we study anther and pollen development of c. pepo and present the cloning and characterisation of a putative pg cppg1 (accession no. hq232488) from pollen cdna in c. pepo. the predicted protein for cppg1 has 416 amino acids, with a high homology to o ...201423879260
xylan-degrading enzymes in male and female flower nectar of cucurbita pepo.nectar is a very complex mixture of substances. some components (sugars and amino acids) are considered primary alimentary rewards for animals and have been investigated and characterized in numerous species for many years. in contrast, nectar proteins have been the subject of few studies and little is known of their function. only very recently have detailed studies and characterization of nectar proteins been undertaken, and then for only a very few species. this current work represents a firs ...201121813563
molecular cloning and characterization of patellin1, a novel sec14-related protein, from zucchini (cucurbita pepo).a full-length patellin1 (patl1) cdna was cloned and characterized from zucchini (cucurbita pepo). patl1, originally discovered in the higher plant arabidopsis thaliana, is a plant sec14-related protein that localizes to the cell plate during the late stages of cytokinesis. patl1 is related in sequence to other eukaryotic proteins involved in membrane trafficking and is thought to participate in vesicle trafficking events associated with cell plate maturation. the zucchini patl1 (cppatl1) cdna pr ...200616542754
cloning, expression and n-terminal myristoylation of cpcpk1, a calcium-dependent protein kinase from zucchini (cucurbita pepo l.).we have isolated a full-length cdna clone (cpcdpk1) encoding a calcium-dependent protein kinase (cdpk) gene from zucchini (cucurbita pepo l.). the predicted amino acid sequence of the cdna shows a remarkably high degree of similarity to members of the cdpk gene family from arabidopsis thaliana, especially atcpk1 and atcpk2. northern analysis of steady-state mrna levels for cpcpk1 in etiolated and light-grown zucchini seedlings shows that the transcript is most abundant in etiolated hypocotyls an ...199910080688
blue light activates a specific protein kinase in higher plants.blue light mediates the phosphorylation of a membrane protein in seedlings from several plant species. when crude microsomal membrane proteins from dark-grown pea (pisum sativum l.), sunflower (helianthus annuus l.), zucchini (cucurbita pepo l.), arabidopsis (arabidopsis thaliana l.), or tomato (lycopersicon esculentum l.) stem segments, or from maize (zea mays l.), barley (hordeum vulgare l.), oat (avena sativa l.), wheat (triticum aestivum l.), or sorghum (sorghum bicolor l.) coleoptiles are i ...199216653043
computer analysis of phytochrome sequences from five species: implications for the mechanism of action.the amino acid sequences of phytochrome from avena sativa, oryza sativa, curcurbita pepo, pisum sativum and arabidopsis thaliana have been analyzed with a variety of computer programs, with a view to identifying areas of the protein which contribute to the properties of this photoreceptor. a region at the c-terminus has been shown to be amphiphilic, and by analogy with surface-seeking peptides, may be responsible for interaction of phytochrome with lipid bilayers. possible targeting sequences in ...20092291773
an antibody to the castor bean glyoxysomal lipase (62 kd) also binds to a 62 kd protein in extracts from many young oilseed plants.an antibody raised against purified glyoxysomal lipase (triacylglycerol hydrolase ec 3.1.1.3.) from castor bean (relative molecular weight of 62,000) also binds to a protein with a relative molecular weight of 62,000 in extracts of food reserve tissues from many young oilseed plants. these plants include brassica napus l., zea mays l., arachis hypogaea l., glycine max l., gossipium hirsutum l., cucurbita pepo l., helianthus annuus l., pisum sativum l., and cicer arietinum l. the antibody caused ...198716665808
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