Publications

dual role of the pigmentation gene b in affecting carotenoid and vitamin e content in squash (cucurbita pepo) mesocarp.	the yellow and orange colorations of the mesocarp of pumpkins and squash of cucurbita pepo are due to the presence of carotenoid pigments that also greatly contribute to their nutritional value. carotenoids, as well as tocopherols (vitamin e), are isoprenoid compounds formed by branches of a common biosynthetic pathway. photodiode array hplc analysis was used to simultaneously determine the content and composition of fruit-flesh carotenoids and tocopherols in five pairs of near-isogenic lines di ...	2005	16332127
accumulation of weathered polycyclic aromatic hydrocarbons (pahs) by plant and earthworm species.	experiments were conducted to assess the bioavailability of polyclycic aromatic hydrocarbons (pahs) in soil from a manufactured gas plant site. three plant species were cultivated for four consecutive growing cycles (28 days each) in soil contaminated with 36.3 microg/g total pah. during the first growth period, cucurbita pepo ssp. pepo (zucchini) tissues contained significantly greater quantities of pahs than did cucumis sativus (cucumber) and cucurbita pepo ssp. ovifera (squash). during the fi ...	2006	16337258
ascorbic acid oxidase is dynamically regulated by light and oxygen. a tool for oxygen management in plants?	ascorbic acid oxidase (aao) is a plant blue-copper protein catalyzing dioxygen reduction to water using ascorbic acid as the electron donor. in spite of extensive molecular characterization the physiological role of aao is still uncertain. abundant mrna, protein and activity of aao were observed in illuminated leaves of cucurbita pepo. aao activity was found to be proportional to light intensity. the light effect was rapidly reversed in dark and activity remained low throughout the dark period. ...	2007	16343690
cucurbitosides f-m, acylated phenolic glycosides from the seeds of cucurbita pepo.	eight new phenolic glycosides, cucurbitosides f-m (1-8), were isolated from the seeds of cucurbita pepo. their structures were elucidated as 4-(2-hydroxyethyl)phenyl 5-o-(2-s-2-methylbutyryl)-beta-d-apiofuranosyl(l-->2)-beta-d-glucopyranoside (1), 4-(2-hydroxyethyl)phenyl 5-o-(3-methylbutyryl)-beta-d-apiofuranosyl(l-->2)-beta-d-glucopyranoside (2), 4-(2-hydroxyethyl)phenyl 5-o-nicotinyl-beta-d-apiofuranosyl(l-->2)-beta-d-glucopyranoside (3), 4-(2-hydroxyethyl)phenyl 5-o-(4-aminobenzoyl)-beta-d-a ...	2005	16378368
selenium species determination in selenium-enriched pumpkin (cucurbita pepo l.) seeds by hplc-uv-hg-afs.	pumpkins were treated by spraying the leaves in the flowering period with a water solution containing 1.5 mg se per liter in the form of na2seo4. the average total selenium content of seeds was found to be 0.19 microg g(-1) in nontreated pumpkins and 1.1 microg g(-1) in exposed ones. for speciation analysis, enzymatic hydrolysis with different amounts of protease xiv was carried out. under optimal conditions of enzymatic hydrolysis, 90% of the total selenium was found in soluble forms. separatio ...	2005	16379394
intracellular adaptations of glutathione content in cucurbita pepo l. induced by treatment with reduced glutathione and buthionine sulfoximine.	the intracellular effects of gsh (reduced glutathione) and bso (buthionine sulfoximine) treatment on glutathione content were investigated with immunogold labeling in individual cellular compartments of cucurbita pepo l. seedlings. generally, gsh treatment led to increased levels of glutathione in roots and leaves (up to 3.5-fold in nuclei), whereas bso treatment significantly decreased glutathione content in all organs. transmission electron microscopy revealed that glutathione levels in mitoch ...	2006	16520878
molecular cloning and characterization of patellin1, a novel sec14-related protein, from zucchini (cucurbita pepo).	a full-length patellin1 (patl1) cdna was cloned and characterized from zucchini (cucurbita pepo). patl1, originally discovered in the higher plant arabidopsis thaliana, is a plant sec14-related protein that localizes to the cell plate during the late stages of cytokinesis. patl1 is related in sequence to other eukaryotic proteins involved in membrane trafficking and is thought to participate in vesicle trafficking events associated with cell plate maturation. the zucchini patl1 (cppatl1) cdna pr ...	2006	16542754
uptake by cucurbitaceae of soil-bome contaminants depends upon plant genotype and pollutant properties.	three cucurbitaceae, cucurbita pepo l. subsp. pepo (cv. black beauty, true zucchini), cucurbita pepo l. intersubspecific cross (cv. zephyr, summer squash), and cucumis sativis (cv. marketmore, cucumber), were grown in rhizotrons containing soil contaminated with three classes of highly weathered, hydrophobic organic contaminants: (1) technical chlordane, (2) dichlorodiphenylethanes (ddt and ddd) and -ethene (dde), (3) polyaromatic hydrocarbons (pahs), and heavy metal residues. movement of the co ...	2006	16570602
a study of the effects of cucurbita pepo seeds on kidney excretion.		1922	16576619
influence of citric acid amendments on the availability of weathered pcbs to plant and earthworm species.	a series of small and large pot trials were conducted to assess the phytoextraction potential of several plant species for weathered polychlorinated biphenyls (pcbs) in soil (105 microg/g arochlor 1268). in addition, the effect of citric acid on pcb bioavailability to both plants and earthworms was assessed. under small pot conditions (one plant, 400 g soil), three cucurbits (cucurbita pepo ssp pepo [zucchini] and ssp ovifera [nonzucchini summer squash], cucumis sativus, cucumber) accumulated up ...	2006	16615308
comparative study on biological parameters of bemisia tabaci (genn.) collected on four host plants from varamin-iran.	during 2003 biological parameters of sweetpotato whitefly, b. tabaci (genn.) (horn. aleyrodidae) as a major pest of field crops, vegetables and ornamentals were studied. in this study, the infested leaves of cucumber (cucumis sativus l.) zucchini (cucurbita pepo l.) eggplant (solanum melongena l.) and cotton (gossypium hirsutum l.) with whitefly nymphs and pupae were collected from varamin-iran, and were transferred to the laboratory. the newly emerged males and females of each population were r ...	2005	16628901
monitoring presence and advance of the alien invasive western corn rootworm beetle in eastern slovenia with highly sensitive metcalf traps.	the american chrysomelid beetle diabrotica virgifera virgifera leconte (d.v.v.), also called the western corn rootworm, spread from the location of its original introduction into europe, belgrade airport (baca 1993), in all directions. within a decade it occupied almost all countries of south-eastern and central europe. however, it reached slovenia as late as 2003: only 19 specimen were found in maize fields of the eastern and also western provinces. already in the summer of 2004, their number h ...	2005	16628904
blue light activates a specific protein kinase in higher plants.	blue light mediates the phosphorylation of a membrane protein in seedlings from several plant species. when crude microsomal membrane proteins from dark-grown pea (pisum sativum l.), sunflower (helianthus annuus l.), zucchini (cucurbita pepo l.), arabidopsis (arabidopsis thaliana l.), or tomato (lycopersicon esculentum l.) stem segments, or from maize (zea mays l.), barley (hordeum vulgare l.), oat (avena sativa l.), wheat (triticum aestivum l.), or sorghum (sorghum bicolor l.) coleoptiles are i ...	1992	16653043
slow degradation of the d1 protein is related to the susceptibility of low-light-grown pumpkin plants to photoinhibition.	photoinhibition of photosystem ii (psii) electron transport and subsequent degradation of the d1 protein were studied in pumpkin (cucurbita pepo l.) leaves developed under high (1000 mumol m(-2) s(-1)) and low (80 mumol m(-2) s(-1)) photon flux densities. the low-light leaves were more susceptible to high light. this difference was greatly diminished when illumination was performed in the presence of chloramphenicol, indicating that a poor capacity to repair photodamaged psii centers is decisive ...	1992	16653122
significance of phloem exudate of cucurbita pepo with reference to translocation of organic materials.		1941	16653692
cytokinins in seeds of pumpkin.	extracts of seeds of pumpkin (cucurbita pepo linn.) contain three chromatographically distinguishable cytokinins which are held on dowex 50-w and are extractable by ethanol and n-butanol. two of the active factors are precipitable by silver nitrate at acidic ph. the chromatographic behavior and the spectral characteristics of one of these cytokinins are similar to those of zeatin. however, the r(f) values of the other two active compounds do not match with those of any of the known natural cytok ...	1970	16657272
incorporation of c-kaurene into the gibberellin of a higher plant (pharbitis nil chois).	enzymic formation of (14)c-kaurene from 2-(14)c-mevalonate was carried out with a cell-free system of cucurbita pepo l. it was shown that either heating of the enzyme system or the addition of the growth retardants (2-chloroethyl)-trimethylammonium chloride and 2'-isopropyl-4' (trimethylammonium chloride)-5'-methylphenyl piperidine-1-carboxylate prevented the synthesis of (14)c-kaurene. experiments in which (14)c-kaurene was applied to seedlings of pharbitis nil revealed that the kaurene is conv ...	1971	16657821
de novo synthesis of phytochrome in pumpkin hooks.	phytochrome becomes density labeled in the hook of pumpkin (cucurbita pepo l.) seedlings grown in the dark on d(2)o, indicating that the protein moiety of the pigment is synthesized de novo during development. red light causes a rapid decline of the total phytochrome level in the hook of etiolated seedlings but upon return to the dark, phytochrome again accumulates. these newly appearing molecules are also synthesized de novo. newly synthesized phytochrome in both dark-grown and red-irradiated s ...	1973	16658511
turnover of phytochrome in pumpkin cotyledons.	by using density labeling, it was found that the protein moiety of phytochrome is synthesized de novo in the red-absorbing form in cotyledons of dark-grown pumpkin (cucurbita pepo l.) seedlings, as well as those irradiated with red light and returned to the dark. the rate of synthesis appears to be unaffected by the light treatment. turnover of the red-absorbing form was also detected in dark grown seedlings using density labeling, while turnover of the far red-absorbing form is already implied ...	1973	16658512
translocation of sucrose by squash plants.	sucrose-(14)c was fed to the leaf blades of squash plants (cucurbita pepo l. var. melopepo torticallis bailey) for 30 or 60 minutes. petioles of treated plants were cut into sections and extracted. the majority of the (14)c within the petiole was in sucrose rather than stachyose, the sugar that is transported by the squash plants when (14)co(2) is supplied. this indicates that the phloem loading system of squash plants is not the system that specifies which sugar is transported.	1973	16658634
the isolation and partial characterization of a membrane fraction containing phytochrome.	if 4-day-old dark-grown zucchini squash seedlings (cucurbita pepo l. cv. black beauty) are exposed briefly to red light, subsequent cell fractionation yields about 40% of the total extractable phytochrome in the far red-absorbing form bound to a particulate fraction. the amount of far red-absorbing phytochrome in the pellet is strongly dependent on the mg concentration in the extraction medium. the apparent density of the pfr-containing particles following sedimentation on sucrose gradients corr ...	1974	16658871
in vivo properties of membrane-bound phytochrome.	after a 3-minute irradiation with red light, which saturates the phototransformation from the red light-absorbing form of phytochrome to the far red light absorbing form of phytochrome, about 40% of the phytochrome extractable from hooks of etiolated squash seedlings (cucurbita pepo l. cv. black beauty) can be pelleted as pfr at 17,000g after 30 minutes. dark controls yield only 2 to 4% pelletable phytochrome in the form pr. if a dark period intervenes between red irradiation and extraction, the ...	1974	16658872
autoradiographic examination of meristems of intact boron-deficient squash roots treated with tritiated thymidine.	intact roots of boron-sufficient squash (cucurbita pepo l.) plants, plants entering boron deficiency, and plants recovering from boron deficiency were exposed to tritiated thymidine at the end of the treatment period to label the replicating dna of root tip cells. using histological sections, autoradiographs of intact root meristems were prepared. the labeling pattern in +b root tips revealed the presence of a well defined quiescent center. the ability of root tip cells to incorporate label is c ...	1974	16658968
l-ornithine:2-oxoacid aminotransferase from squash (cucurbita pepo, l.) cotyledons: purification and properties.	ornithine: 2-oxoacid aminotransferase (ec 2.6.1.13) has been purified over 400-fold with a total recovery of 14% from acetone powders of cotyledons of germinating squash (cucurbita pepo, l.) seedlings. the ph optimum of the transamination between l-ornithine and alpha-ketoglutarate is 8 and the michaelis constants are 4.7 mm and 6.3 mm, respectively. the enzyme has a molecular weight of 48,000 as determined by gel filtration. the reaction is essentially specific for alpha-ketoglutarate as the am ...	1975	16659110
red light-enhanced phytochrome pelletability: re-examination and further characterization.	red light-enhanced pelletability of phytochrome was observed in extracts of all 11 plants tested: avena sativa l., secale cereale l., zea mays l., cucurbita pepo l., sinapis alba l., pisum sativum l., helianthus anuus l., raphanus sativus l., glycine max (l.) merr., phaseolus vulgaris l., and lupinus albus l. this enhanced pelletability was observed in all 11 plants following in situ irradiation (in vivo binding) but only in sinapis and cucurbita after irradiation of crude extracts (in vitro bin ...	1976	16659745
differential effect of irradiance and nutrient nitrate on the relationship of in vivo and in vitro nitrate reductase assay in chlorophyllous tissues.	growth at increasing continuous irradiance (at high nutrient nitrate) and nutrient nitrate concentrations (at high continuous irradiance) furnished increases in the in vivo and in vitro nitrate reductase activities of corn (zea mays l.), field peas (pisum arvense l.), wheat (triticum aestivum l.), barley (hordeum vulgare l.), and globe amaranth (gomphrena globosa l.) leaves and of marrow (cucurbita pepo l.) cotyledons. ratios of in vivo to in vitro activity declined exponentially in all species ...	1977	16659888
effect of boron on cell elongation and division in squash roots.	this work establishes that cessation of root elongation of intact squash (cucurbita pepo l.) plants is an early result of boron deficiency. root elongation is slowed by 6 hours and is virtually stopped as early as 24 hours after boron is first withheld from the nutrient solution. as root elongation ceased, cell elongation progressed distally into the region normally occupied by the apical meristem and eventually the meristem became indistinguishable. differentiation was determined by use of an e ...	1977	16659961
early effects of boron deficiency on indoleacetic acid oxidase levels of squash root tips.	the indoleacetic acid (iaa) oxidase activity of root tips of boron-sufficient, -deficient, recovering, and iaa-treated boron-sufficient squash plants (cucurbita pepo l.) was determined. apical and subapical root sections displayed an increase in iaa oxidase activity between 6 and 9 hours after boron was withheld, and after 24 hours the activity of the apical sections showed a 20-fold increase over +b controls. root elongation of -b plants was inhibited before an increase in oxidase activity coul ...	1977	16659990
phloem loading in squash.	squash (cucurbita pepo l. var. melopepo torticalis, bailey) leaves were supplied with (14)c-sucrose, then specific radioactivities of the glucose and galactose moieties of translocated stachyose were determined. in every case, the specific radioactivity of the galactose moiety was greater than that of the glucose moiety. it is concluded that the stachyose was not synthesized at either the phloem-loading site or subsequent to phloem loading, but rather in cells that were not a part of the translo ...	1977	16660138
light-dependent emission of hydrogen sulfide from plants.	with the aid of a sulfur-specific flame photometric detector, an emission of volatile sulfur was detected from leaves of cucumber (cucumis sativus l.), squash and pumpkin (cucurbita pepo l.), cantaloupe (cucumis melo l.), corn (zea mays l.), soybean (glycine max [l.] merr.) and cotton (gossypium hirsutum l.). the emission was studied in detail in squash and pumpkin. it occurred following treatment of the roots of plants with sulfate and was markedly higher from either detached leaves treated via ...	1978	16660257
purification and kinetics of higher plant nadh:nitrate reductase.	squash cotyledon (cucurbita pepo l.) nadh:nitrate reductase (nr) was purified 150-fold with 50% recovery by a single step procedure based on the affinity of the nr for blue-sepharose. blue-sepharose, which is prepared by direct coupling of cibacron blue to sepharose, appears to bind squash nr at the nadh site. the nr can be purified in 2 to 3 hours to a specific activity of 2 mumol of nadh oxidized/minute * milligram of protein. corn (zea mays l.) leaf nr was also purified to a specific activity ...	1978	16660347
mechanisms of resistance to sulfur dioxide in the cucurbitaceae.	the relative resistance of four cultivars of the cucurbitaceae (cucumis sativus l. cv. national pickling, and inbred line sc 25; cucurbita pepo l. cv. prolific straightneck squash, and cv. small sugar pumpkin) to so(2) was determined. according to plots of the degree of exposure to so(2) (which depends on the so(2) concentration and the duration of the exposure), there is an 8-fold difference in resistance to this toxic gas among these cultivars. however, if the degree of injury is plotted as a ...	1978	16660380
distribution of alpha-galactosidase in cucurbita pepo.	the distribution of alpha-galactosidase (alpha-d-galactoside galactohydrolase [ec 3.2.1.22]) in cucurbita pepo has been determined in an attempt to assess its involvement in hydrolysis of transport sugars of the raffinose oligosaccharide series ([alpha-1-6-0-galactopyranosyl](n) sucrose). extracts prepared from leaves and petioles at different stages of development, roots, flowers, dry and germinating seeds, all contained appreciable levels of alpha-galactosidase activity. chromatography of thes ...	1978	16660591
regulation of pyrimidine biosynthesis in intact cells of cucurbita pepo.	the occurrence of the complete orotic acid pathway for the biosynthesis de novo of pyrimidine nucleotides was demonstrated in the intact cells of roots excised from summer squash (cucurbita pepo l. cv. early prolific straightneck). evidence that the biosynthesis of pyrimidine nucleotides proceeds via the orotate pathway in c. pepo included: (a) demonstration of the incorporation of [(14)c]nahco(3), [(14)c]carbamylaspartate, and [(14)c]orotic acid into uridine nucleotides; (b) the isolation of [( ...	1979	16661010
photosynthesis, growth, and ultraviolet irradiance absorbance of cucurbita pepo l. leaves exposed to ultraviolet-b radiation (280-315 nm).	net photosynthesis, growth, and ultraviolet (uv) radiation absorbance were determined for the first leaf of cucurbita pepo l. exposed to two levels of uv-b irradiation and a uv-b radiation-free control treatment. absorbance by extracted flavonoid pigments and other uv-b radiation-absorbing compounds from the first leaves increased with time and level of uv-b radiation impinging on leaf surfaces. although absorbance of uv-b radiation by extracted pigments increased substantially, uv-b radiation a ...	1981	16661610
synthesis, salvage, and catabolism of uridine nucleotides in boron-deficient squash roots.	previous work has provided evidence that plants may require boron to maintain adequate levels of pyrimidine nucleotides, suggesting that the state of boron deficiency may actually be one of pyrimidine starvation. since the availability of pyrimidine nucleotides is influenced by their rates of synthesis, salvage, and catabolism, we compared these activities in the terminal 3 centimeters of roots excised from boron-deficient and -sufficient squash plants (cucurbita pepo l.). transferring 5-day-old ...	1981	16662114
immunopurification and initial characterization of dicotyledonous phytochrome.	antiserum was prepared against proteolytically undegraded phytochrome obtained from etiolated zucchini squash (cucurbita pepo l., cv. black beauty). the antiserum was prepared by injecting into a rabbit immunoprecipitates between zucchini phytochrome and specific antiserum against undegraded oat (avena sativa l., cv. garry) phytochrome. specific antiphytochrome immunoglobulins were purified from this crude serum by an affinity column consisting of conventionally purified undegraded pea phytochro ...	1982	16662209
stimulation of h(2)s emission from pumpkin leaves by inhibition of glutathione synthesis.	the effect of inhibitors of glutathione (gsh) synthesis, namely gamma-methyl glutamic acid, d-glutamic acid, cystamine, methionine-s-sulfoximine (msx), buthionine-s-sulfoximine, and gsh itself, on the emission of h(2)s was investigated. all these compounds stimulated h(2)s emission from pumpkin (cucurbita pepo l. cv small sugar pumpkin) leaf discs in response to sulfate. msx and gsh were the most effective compounds, stimulating h(2)s emission from leaf discs of mature pumpkin leaves by about 80 ...	1982	16662293
emission of hydrogen sulfide by leaf tissue in response to l-cysteine.	leaf discs and detached leaves exposed to l-cysteine emitted a volatile sulfur compound which was proven by gas chromatography to be h(2)s. this phenomenon was demonstrated in all nine species tested (cucumis sativus, cucurbita pepo, nicotiana tabacum, coleus blumei, beta vulgaris, phaseolus vulgaris, medicago sativa, hordeum vulgare, and gossypium hirsutum). the emission of volatile sulfur by cucumber leaves occurred in the dark at a similar rate to that in the light. the emission of leaf discs ...	1982	16662510
comparative phytochrome immunochemistry as assayed by antisera against both monocotyledonous and dicotyledonous phytochrome.	preparation and characterization of antisera against lettuce (lactuca sativa l., cv. grand rapids) and pea (pisum sativum l., cv. alaska) phytochrome is described. these antisera, together with previously obtained antisera against zucchini (cucurbita pepo l., cv. black beauty) and oat (avena sativa l., cv. garry) phytochrome, were used to compare by ouchterlony double immunodiffusion phytochrome isolated from etiolated lettuce, pea, bean (phaseolus vulgaris l., cv. taylor horticultural bush), zu ...	1982	16662599
developmental changes in the potential for h(2)s emission in cucurbit plants.	based on results obtained with leaf discs exposed to sulfate, leaves on cucurbit plants (cucurbita pepo l. cv small sugar pumpkin and cucumis sativus cv chipper) 1 to 2.5 weeks old have a low potential for h(2)s emission (less than 10 picomoles per min per cm(2) leaf area) in response to sulfate, whereas discs from most of the leaves on plants 3 to 4 weeks old emit h(2)s at a higher rate (50 to 150 picomoles per min per cm(2) leaf area). this difference is determined by the age of the plant, and ...	1983	16662816
partial purification and properties of an alkaline alpha-galactosidase from mature leaves of cucurbita pepo.	a fourth molecular from of alpha-galactosidase, designated l(iv), an alkaline alpha-galactosidase, was isolated from leaves of cucurbita pepo and purified 165-fold. it was active over a narrow ph range with optimal hydrolysis of p-nitrophenyl-alpha-d-galactoside and stachyose at ph 7.5. the rate of stachyose hydrolysis was 10 times that of raffinose. k(m) determinations in mcilvaine buffer (200 millimolar na(2)-phosphate, 100 millimolar citric acid, ph 7.5) for p-nitrophenyl-alpha-d-galactoside, ...	1983	16662884
heterogeneity of pumpkin ribosomal dna.	the ribosomal dna (rdna) of cucurbita pepo l. has been found to consist of tandemly arrayed repeat units, most of which are 10 kilobases in length. thirty-six repeat units, cloned into the hindiii site of pacyc 177, fall into seven classes which differ from each other in length and/or nucleotide sequence. most of the heterogeneity occurs in noncoding portions of the repeat unit although there is some nucleotide sequence variation in the coding portion as well. heterogeneity of base modification ...	1983	16662953
role of o-acetylserine in hydrogen sulfide emission from pumpkin leaves in response to sulfate.	in the presence of excess sulfate, cysteine synthesis in pumpkin (cucurbita pepo) leaves is not limited by sulfate reduction, but by the availability of o-acetylserine. feeding of o-acetylserine or its metabolic precursors s-acetyl-coenzyme-a and coenzyme a to leaf discs enhanced the incorportion of [(35)s]sulfate into reduced sulfur compounds, mainly into cysteine, at the cost of lowered h(2)s emission; the uptake and reduction of sulfate is not affected by these treatments. beta-fluoropyruvate ...	1983	16663258
de novo purine biosynthesis in intact cells of cucurbita pepo.	the capacity of intact cells of roots excised from summer squash plants (cucurbita pepo l. cv early prolific straightneck) to synthesize purine nucleotides de novo was investigated. evidence that purine nucleotides are synthesized de novo included: (a) demonstration of the incorporation of [1-(14)c]glycine, [2-(14)c]glycine, nah(14)co(3), and h(14)coona into total adenine nucleotides; (b) observation that the addition of azaserine or aminopterin, known inhibitors of de novo purine synthesis in o ...	1983	16663298
characterization by enzyme-linked immunosorbent assay of monoclonal antibodies to pisum and avena phytochrome.	nine monoclonal antibodies to pea (pisum sativum l.) and 16 to oat (avena sativa l.) phytochrome are characterized by enzyme-linked immunosorbent assay against phytochrome from six different sources: pea, zucchini (cucurbita pepo l.), lettuce (lactuca sativa l.), oat, rye (secale cereale l.), and barley (hordeum vulgare l.). all antibodies were raised against phytochrome with a monomer size near 120,000 daltons. nevertheless, none of them discriminated qualitatively between 118/114-kilodalton oa ...	1984	16663365
aspartate carbamyltransferase : site of end-product inhibition of the orotate pathway in intact cells of cucurbita pepo.	lovatt et al. (1979 plant physiol 64: 562-569) have previously demonstrated that end-product inhibition functions as a mechanism regulating the activity of the orotic acid pathway in intact cells of roots excised from 2-day-old squash plants (cucurbita pepo l. cv early prolific straightneck). uridine (0.5 millimolar final concentration) or one of its metabolites inhibited the incorporation of nah(14)co(3), but not [(14)c]carbamylaspartate or [(14)c]orotic acid, into uridine nucleotides (sigmaump ...	1984	16663656
cytokinin-modulated gene expression in excised pumpkin cotyledons.	comparison of two-dimensional polyacrylamide gel electrophoretic maps of proteins isolated from benzyladenine-treated and untreated pumpkin (cucurbita pepo l. cv halloween) cotyledons showed that the expression of certain proteins is enhanced, induced, or suppressed by the cytokinin treatment. the amount of poly(a)(+) mrna isolated from cotyledons incubated with 10(-4) molar benzyladenine for five days was about four-fold over the water-incubated control. the activity of hydroxypyruvate reductas ...	1985	16664037
spectral characterization and proteolytic mapping of native 120-kilodalton phytochrome from cucurbita pepo l.	a spectral, immunochemical, and proteolytic characterization of native 120-kilodalton (kd) phytochrome from cucurbita pepo l. is presented and compared with that previously reported for native 124-kd phytochrome from avena sativa. the molecule was partially purified ( approximately 200-fold) in the phytochrome-far red-absorbing form (pfr) in the presence of the protease inhibitor, phenylmethylsulfonyl fluoride, using a modification of the procedure initially developed to purify 124-kd avena phyt ...	1985	16664177
increased arginine biosynthesis during phosphorus deficiency : a response to the increased ammonia content of leaves.	the accumulation of arginine in leaves of four citrus rootstock cultivars during p deficiency has been demonstrated to be due to increased de novo synthesis rather than decreased catabolism or increased protein degradation (e rabe, cj lovatt, 1984, plant physiol 76: 747-752). in this report, we provide evidence (a) that the increased activity of the arginine biosynthetic pathway observed for citrus rootstocks grown under p-deficient conditions for 7 months is due to an increase in the concentrat ...	1986	16664901
the response of foliar gas exchange to exogenously applied ethylene.	the responsiveness to ethylene of net photosynthesis and stomatal conductance to water vapor in intact plants was investigated in 13 herbaceous species representing seven plant families. exposures were conducted in an open, whole-plant exposure system providing controlled levels of irradiance, air temperature, co(2), relative humidity, and ethylene concentration. net photosynthesis and stomatal conductance to water vapor in units of moles per square meter per second were measured on recently exp ...	1986	16665086
metabolic requirement of cucurbita pepo for boron.	lateral roots of intact summer squash seedlings (cucurbita pepo l.) were used to quantify the effects of boron deficiency on dna synthesis, protein synthesis, and respiration. the temporal relationship between changes in these metabolic activities and the cessation of root elongation caused by boron deprivation was determined. transferring 5-day-old squash seedlings to a hydroponic culture medium without boron for 6 hours resulted in a 62% reduction in net root elongation and a 30% decrease in t ...	1987	16665231
5'-azido-n-1-napthylphthalamic acid, a photolabile analog of n-1-naphthylphthalamic acid : synthesis and binding properties in curcurbita pepo l.	a photolabile analog of n-1-naphthylphthalamic acid (npa), 5'-azido-n-1-naphthylphthalamic acid (az-npa), has been synthesized and characterized. this potential photoaffinity label for the plasma membrane npa binding protein competes with [(3)h]npa for binding sites on curcurbita pepo l. (zucchini) hypocotyl cell membranes with k(0.5) = 2.8 x 10(-7) molar. the k(0.5) for npa under these conditions is 2 x 10(-8) molar, indicating that the affinity of az-npa for the membranes is only 14-fold lower ...	1987	16665662
solubilization and purification of nad(p)h dehydrogenase of cucurbita microsomes.	an nad(p)h dehydrogenase stimulated by quinone (p pupillo, v valenti, l de luca, r hertel 1986 plant physiol 80: 384-389) was solubilized from washed microsomes of zucchini squash hypocotyls (cucurbita pepo l.) by use of 1% triton x-100. the solubilized enzyme remained in solution in aqueous buffer and could be purified by a combination of sepharose 6b chromatography and blue ultrogel chromatography. of the three peaks of activity eluted from the latter column with a salt gradient, peak 3 had 50 ...	1987	16665785
an antibody to the castor bean glyoxysomal lipase (62 kd) also binds to a 62 kd protein in extracts from many young oilseed plants.	an antibody raised against purified glyoxysomal lipase (triacylglycerol hydrolase ec 3.1.1.3.) from castor bean (relative molecular weight of 62,000) also binds to a protein with a relative molecular weight of 62,000 in extracts of food reserve tissues from many young oilseed plants. these plants include brassica napus l., zea mays l., arachis hypogaea l., glycine max l., gossipium hirsutum l., cucurbita pepo l., helianthus annuus l., pisum sativum l., and cicer arietinum l. the antibody caused ...	1987	16665808
the regulation of gelation of phloem exudate from cucurbita fruit by dilution, glutathione, and glutathione reductase.	the average glutathione equivalent concentration in phloem exudate collected from squash fruit (cucurbita moschata [duchesne] poir. var butternut) and pumpkin fruit (cucurbita pepo [l.] var jack-o-lattern) was 1.02 and 0.60 millimolar, respectively. glutathione reductase (ec 1.6.4.2) activity in phloem exudate from squash and pumpkin fruit averaged 0.48 and 1.74 micromole nadph oxidized per minute per milliliter, respectively. protein concentrations in fruit phloem exudates averaged 67 milligram ...	1988	16666036
rearrangement of the chloroplast thylakoid at chilling temperature in the light.	the leaves of chilling-sensitive pumpkin (cucurbita pepo l.) showed symptoms reminiscent of photoinhibition when kept for 4 days at 5 degrees c in moderate light. a decrease was observed in the variable part of chlorophyll alpha fluorescence, apparent quantum yield, and maximum rate of o(2) evolution. chloroplast whole-chain electron transport activity measured from chloroplast thylakoids had decreased to 51% of the control value. photosystem ii (psii) activity decreased by only 9%, suggesting t ...	1988	16666221
amino acid transport into membrane vesicles isolated from zucchini : evidence of a proton-amino acid symport in the plasmalemma.	several lines of evidence with intact tissues suggest amino acid transport is mediated by a proton-amino acid symport (l rheinhold, a kaplan 1984 annu rev plant physiol 35: 45-83). however, biochemical studies of proton-coupled amino acid transport in isolated membrane vesicles have not been reported. in the experiments presented here, amino acid transport was studied in membrane vesicles isolated from zucchini (cucurbita pepo l. cv black beauty) hypocotyls. an imposed ph gradient (basic interio ...	1988	16666332
red light-induced accumulation of ubiquitin-phytochrome conjugates in both monocots and dicots.	phytochrome is rapidly degraded in vivo after photoconversion from the stable red-absorbing (pr) form to the far red-absorbing (pfr) form. previously, we have shown in etiolated oat seedlings that ubiquitin-phytochrome conjugates (ub-p) appear after pfr formation suggesting that oat phytochrome is rapidly degraded by a ubiquitin-dependent proteolytic pathway. here, we extend this observation to etiolated tissue from other monocotyledonous (corn [zea mays. (l.)] and rye [secale cereale (l.)] and ...	1989	16666778
b-type cytochromes in higher plant plasma membranes.	the composition and characteristics of b-type cytochromes from higher plant plasma membranes, purified using aqueous two-phase partitioning, were investigated. at least three different cytochromes were identified by their wavelength maxima and redox midpoint potentials (e(0)'). cytochrome b-560.7 (e(0)' from + 110 to + 160 millivolts) was present in zucchini (cucurbita pepo) hypocotyls and bean (phaseolus vulgaris l.) hooks, although in different concentrations. the main component in cauliflower ...	1989	16666854
the nature of light-induced inhibition of photosystem ii in pumpkin (cucurbita pepo l.) leaves depends on temperature.	attached leaves of pumpkin (cucurbita pepo l.) were treated in high or moderate light at room temperature or a 1 degrees c. the symptoms of photoinhibition appearing during light treatments at room temperature could be attributed to a decrease in the primary activity of psii. however, when the light treatment was given at 1 degrees c, the quantum yield of photosynthetic oxygen evolution decreased much more than would be expected from the decrease in the ratio of variable to maximum fluorescence ...	1989	16667113
distribution of chlorophyll-protein complexes during chilling in the light compared with heat-induced modifications.	the effects of chilling in the light (4 days at 5 degrees c and 100-200 micromoles of photons per square meter per second) on the distribution of chlorophyll (chl) protein complexes between appressed and nonappressed thylakoid regions of pumpkin (cucurbita pepo l.) chloroplasts were studied and compared with the changes occurring during in vitro heat treatment (5 minutes at 40 degrees c) of isolated thylakoids. both treatments induced an increase (18 and 65%, respectively) in the relative amount ...	1990	16667464
phytochrome-mediated detection of changes in reflected light.	measurements of phytochrome photoequilibria and photoconversion rates in vivo, in seedlings of cucurbita pepo l. exposed to light in growth chambers, indicate that significant changes in the state of phytochrome can be brought about by changes in the quality and quantity of the light reflected from the walls of the growth chambers. the changes in reflected light, although large, were small in terms of the total radiation (direct light from the lamps plus wall-reflected light) to which the seedli ...	1991	16667942
expression of ascorbic acid oxidase in zucchini squash (cucurbita pepo l.).	the expression of ascorbic acid oxidase was studied in zucchini squash (cucurbita pepo l.), one of the most abundant natural sources of the enzyme. in the developing fruit, specific activity of ascorbic acid oxidase was highest between 4 and 6 days after anthesis. protein and mrna levels followed the same trend as enzyme activity. highest growth rate of the fruit occurred before 6 days after anthesis. within a given fruit, ascorbic acid oxidase activity and mrna level were highest in the epiderm ...	1991	16668145
purification and characterization of galactinol synthase from mature zucchini squash leaves.	galactinol synthase catalyzes the first committed step in the biosynthesis of raffinose sugars. previous attempts to purify the enzyme have proven difficult and have resulted in low quantities of unpurified enzyme. galactinol synthase was purified 752-fold from mature zucchini (cucurbita pepo l. cv burpee hybrid) leaves using sequential liquid chromatography on de 52, octyl-sepharose cl-4b, and sephacryl s-200. this isolation scheme resulted in an 18.6% recovery of the initial activity. the puri ...	1991	16668244
small light-harvesting antenna does not protect from photoinhibition.	high-light-induced decrease in photosystem ii (psii) electron transfer activity was studied in high- and low-light-grown pumpkin (cucurbita pepo l.) plants in vivo and in vitro. the psii light-harvesting antenna of the low-light leaves was estimated to be twice as big as that of the high-light leaves. the low-light leaves were more susceptible to photoinhibition in vivo. however, thylakoids isolated from these two plant materials were equally sensitive to photoinhibition when illuminated in the ...	1991	16668423
a rapid method for isolating glandular trichomes.	a physical method is described for the rapid isolation of plant trichomes, with emphasis on stalked glandular types. the technique involved breaking frozen trichomes with powdered dry ice and collection of glandular heads by sieving from larger tissue fragments. this method was applied to several plants that bear similar stalked trichomes: geranium (pelargonium), potato (solanum tuberosum), tomato (lycopersicon esculentum), squash (cucurbita pepo), and velvetleaf (abutilon theophrasti). the tiss ...	1992	16668834
proton transport and phosphorylation of tonoplast polypeptides from zucchini are stimulated by the phospholipid platelet-activating factor.	the ether phospholipid platelet-activating factor and certain similar phospholipids, including lysophosphatidylcholine, are known to stimulate both h(+) transport and protein phosphorylation in plant microsomal membranes. in the present work, several polypeptides in highly purified tonoplast membranes from zucchini (cucurbita pepo l.) showed platelet-activating factor-dependent phosphorylation. comparison of protein phosphorylation in different membrane fractions separated by sucrose step densit ...	1992	16669084
first known image of cucurbita in europe, 1503-1508.	the genus cucurbita (pumpkin, squash, gourd) is native to the americas and diffused to other continents subsequent to the european contact in 1492. for many years, the earliest images of this genus in europe that were known to cucurbit specialists were the two illustrations of c. pepo pumpkins that were published in fuchs' de historia stirpium, 1542. images of fruits of two cucurbita species, drawn between 1515 and 1518, were recently discovered in the villa farnesina in rome.	2006	16687431
soil amendments, plant age, and intercropping impact p,p'-dde bioavailability to cucurbita pepo.	field experiments were conducted to optimize the phytoextraction of weathered p,p'-dde (p,p'-dichlorodiphenyldichloroethylene) by cucurbita subspecies. the effects of two soil amendments, mycorrhizae or a biosurfactant, on p,p'-dde accumulation was determined. also, p,p'-dde uptake was assessed during plant growth (12, 26, 38, and 62 d), and cultivars that accumulate weathered p,p'-dde were intercropped with cultivars known not to have that ability. cucurbita pepo l. ssp. pepo accumulated large ...	2013	16738383
effect of mycorrhizal fungi on the phytoextraction of weathered p,p-dde by cucurbita pepo.	field experiments were conducted to assess the impact of inoculation with mycorrhizal fungi on the accumulation of weathered p,p'-dde from soil by three cultivars of zucchini (cucurbita pepo spp. pepo cv costata romanesco, goldrush, raven). three commercially available mycorrhizal products (biovam, myco-vam, invam) were inoculated into the root system of the zucchini seedlings at planting. in agreement with our previous findings, plants not inoculated with fungi accumulated large but variable am ...	2006	16777321
extracts from pygeum africanum and other ethnobotanical species with antiandrogenic activity.	extracts from pygeum africanum, serenoa repens and cucurbita pepo are used in the treatment of benign prostatic hyperplasia (bph) and prostate cancer (pca). the activity of the androgen receptor (ar) is known to control growth of the prostate. here, we examined extracts of these plants for their antiandrogenic activity using an ar responsive reporter gene assay for drug discovery. a selective dichloromethane extract from the stem barks of pygeum africanum revealed the highest antiandrogenic effe ...	2006	16783690
in vitro antioxidative activity of pumpkin seed (cucurbita pepo) protein isolate and its in vivo effect on alanine transaminase and aspartate transaminase in acetaminophen-induced liver injury in low protein fed rats.	the antioxidative effects of pumpkin seed protein isolate (cucurbita pepo) were investigated in vitro. the isolate exhibited about 80% radical scavenging activity, chelating activity of approximately 64% on fe2+ ions and an inhibition of approximately 10% of xanthine oxidase. subsequently the effects of the isolate on the plasma activity levels of alanine transaminase and aspartate transaminase against acetaminophen induced acute liver injury in low-protein fed male sprague-dawley rats were asce ...	2006	16807884
inhibition of testosterone-induced hyperplasia of the prostate of sprague-dawley rats by pumpkin seed oil.	the oil from the pumpkin (cucurbita pepo) seed is claimed to be useful in the management of benign prostatic hyperplasia. this investigation seeks to examine the effect of pumpkin seed oil on testosterone-induced hyperplasia of the prostate of rats. hyperplasia was induced by subcutaneous administration of testosterone (0.3 mg/100 g of body weight) for 20 days. simultaneous oral administration of either pumpkin seed oil (2.0 and 4.0 mg/100 g of body weight) or corn oil (vehicle) was also given f ...	2006	16822218
compensatory capsid protein mutations in cucumber mosaic virus confer systemic infectivity in squash (cucurbita pepo).	cucumber mosaic virus (cmv) systemically infects both tobacco and zucchini squash. cmv capsid protein loop mutants with single-amino-acid substitutions are unable to systemically infect squash, but they revert to a wild-type phenotype in the presence of an additional, specific single-site substitution. the d118a, t120a, d192a, and d197a loop mutants reverted to a wild-type phenotype but did so in combination with p56s, p77l, a162v, and i53f or t124i mutations, respectively. the possible effect o ...	2006	16840352
immunocytochemical localization of glutathione precursors in plant cells.	in plant cells, the antioxidant glutathione is synthesized out of its constituents cysteine, glutamate and glycine. as information about their subcellular and cellular distribution was not available in the past, the aim of the present study was to develop a technique that would allow visualization of their distribution in plant cells. with selective antibodies against cysteine, glutamate and glycine, it was possible, for the first time, to study the relative distribution of these components in m ...	2006	16861702
nectar resorption and translocation in cucurbita pepo l. and platanthera chlorantha custer (rchb.).	nectar resorption and sugar translocation were studied in cucurbita pepo (cucurbitaceae) and platanthera chlorantha (orchidaceae) by micro-autoradiography. in both species, nectar was resorbed in pollinated and unpollinated flowers and ovules developing into seeds were found to be the main sugar sink. in c. pepo, the mobility of resorbed sugars in pollinated female flowers was higher than in unpollinated ones; male flowers showed lower mobility of resorbed sugar. in p. chlorantha, radioactivity ...	2007	16883483
eastern north america as an independent center of plant domestication.	the status of eastern north america as an independent center of plant domestication has recently been called into question by a number of genetic and archaeological studies, which suggest that the region may not have witnessed the independent domestication of local crop plants, but rather may have been on the receiving end of domesticated crop plants introduced from mexico. here, i provide a synthesis of the currently available archaeological and genetic evidence from both eastern north america ...	2006	16894156
phytoextraction of excess soil phosphorus.	in the search for a suitable plant to be used in p phytoremediation, several species belonging to legume, vegetable and herb crops were grown in p-enriched soils, and screened for p accumulation potentials. a large variation in p concentrations of different plant species was observed. some vegetable species such as cucumber (cucumis sativus) and yellow squash (cucurbita pepo var. melopepo) were identified as potential p accumulators with >1% (dry weight) p in their shoots. these plants also disp ...	2007	16904249
antioxidative effects of pumpkin seed (cucurbita pepo) protein isolate in ccl4-induced liver injury in low-protein fed rats.	the effects of pumpkin seed (cucurbita pepo) protein isolate on the plasma activity levels of catalase (ca), superoxide dismutase (sod), glutathione peroxidase (gshpx) and total antioxidant capacity (tac) as well as glucose-6-phosphatase (g6pase) in liver homogenates and lipid peroxidation (lpo-malondialdehyde-mda) levels in liver homogenates and liver microsomal fractions against carbon tetrachloride (ccl(4))-induced acute liver injury in low-protein fed sprague-dawley rats (rattus norvegicus) ...	2006	16909447
methyl jasmonate is a more effective senescence-promoting factor in cucurbita pepo (zucchini) cotyledons when compared with darkness at the early stage of senescence.	the effects of short-term darkening and methyl jasmonate (meja) on cotyledon senescence were studied 24h after transfer of intact 7-day-old cucurbita pepo (zucchini) seedlings to darkness or spraying with 100 microm meja. the jasmonate inhibitory effect on chlorophyll content and chloroplast transcriptional activity was stronger compared with darkness. further, meja reduced the photosynthetic rate whereas darkness did not affect photosynthesis. neither stress factor affected the photochemical qu ...	2007	16987568
fatty acids by high-performance liquid chromatography and evaporative light-scattering detector.	a high-performance liquid chromatographic (hplc) separation method with an evaporative light-scattering detector (elsd) has been developed for the separation and quantitative analysis of fatty acid methyl esters (fame) in three different oils. reverse-phased c18 hplc separation of 13 fame is achieved using a methanol/water eluent mixture. the retention times (rt) reflect the elution behavior of these compounds on c18 reversed-phase hplc. the proposed method is tested on: soybean oil (glycine max ...	2006	17007865
ultrasonic treatment stimulates multiple shoot regeneration and explant enlargement in recalcitrant squash cotyledon explants in vitro.	ultrasonic treatment (0.5-2 min) stimulated multiple shoot regeneration to high levels in vitro from recalcitrant cotyledon explants of commercial squash (cucurbita pepo l.) cultivars ma'yan and bareqet, on murashige and skoog [physiol plant 15:473-497, 1962] (regeneration) medium augmented with 4.4 microm benzyladenine. at this stage, unsonicated control explants regenerated only a few very small shoots or bud-like structures. ultrasound also stimulated massive explant growth. ultrasound treatm ...	2007	17021848
potential for phytoremediation of polychlorinated biphenyl-(pcb-)contaminated soil.	weathered soils contaminated with commercial-grade aroclor 1260 from three sites in canada were used to investigate the polychlorinated biphenyl (pcb) phytoextraction potential of nine plant species (festuca arundinacea, glycine max, medicago sativa, phalaris arundinacea, lolium multiflorum, carex normalis, and three varieties of cucurbita pepo ssp. pepo) under controlled greenhouse conditions. the soils used varied in pcb concentration (90-4200 microg/g) and total organic content (0.06-2.02%). ...	2006	17120525
growth conditions impact 2,2-bis(p-chlorophenyl)-1,1-dichloroethylene (p,p'-dde) accumulation by cucurbita pepo.	laboratory experiments were conducted to study the effects of soil moisture content, planting density, plant age, and the growth of multiple generations on the bioconcentration of weathered p,p'-dde by the plant cucurbita pepo. as soil moisture content increased from 7.4% to 29.9% (by weight), rates of contaminant accumulation by plant roots were increased by more than a factor of 2. higher planting density also led to higher uptake, as the root bioconcentration factor (bcf, dry-weight ratio of ...	2006	17120529
isotopic enrichment in a phloem-feeding insect: influences of nutrient and water availability.	the isotopic enrichment between an animal and its diet can vary among and within living systems, but the sources of variation are not yet fully understood. some studies have found that diet quality or an animal's nutritional status can influence the degree of trophic enrichment, while others have dismissed nutrition as a contributing factor. we evaluated the effects of nutrient and water availability on carbon and nitrogen isotopic enrichment in a specialized plant-herbivore system. aphids are l ...	2007	17124569
artificial elevation of glutathione affects symptom development in zymv-infected cucurbita pepo l. plants.	styrian oil pumpkin seedlings (cucurbita pepo l. subsp. pepo var. styriaca greb: .) were treated for 48 h with 1 mm otc (l-2-oxothiazolidine-4-carboxylic acid) in order to artificially increase cellular glutathione content. they were inoculated with zucchini yellow mosaic virus (zymv) 10 days later. the effects of otc treatment and zymv infection on glutathione levels were examined at the subcellular level by immunogold labeling of glutathione using a transmission electron microscope (tem). thes ...	2007	17143780
virus-induced changes in the subcellular distribution of glutathione precursors in cucurbita pepo (l.).	changes in glutathione contents occur in plants during environmental stress situations, such as pathogen attack, as the formation of reactive oxygen species leads to the activation of the antioxidative defence system. as glutathione is synthesized out of its constituents cysteine, glycine, and glutamate the availability of these components will limit glutathione synthesis in plants especially during stress situations and therefore the ability of the plant to fight oxidative stress. to gain a dee ...	2007	17143806
reactions, characterization and uptake of ammoxidized kraft lignin labeled with 15n.	ammoxidation of kraft lignin was carried out in a parr reactor using (15)nh(3) as the main nitrogen source. reaction parameters were set up until a total nitrogen content of approximately 13 wt.% in lignin was achieved, in accordance with conditions of previous studies. analytical tools such as ftir, py-gc/ms, and solid state nmr were used in this research. the nature of nitrogen bondings is discussed. the incorporation of the (15)n from ammoxidized lignin was followed in pumpkins (zucchini cucu ...	2007	17161600
comparing contributions of soil versus root colonization to variations in stomatal behavior and soil drying in mycorrhizal sorghum bicolor and cucurbita pepo.	in prior studies we learned that colonization of soil can be as important as colonization of roots in determining mycorrhizal influence on the water relations of host plants. here we use a path analysis modeling approach to test (a) whether quantity of hyphae in soil contributes to variations in stomatal behavior and soil drying, and (b) whether soil colonization or root colonization has a stronger influence on these stomatal and soil drying responses. experiments were performed on sorghum bicol ...	2007	17189660
differences in p,p'-dde bioaccumulation from compost and soil by the plants cucurbita pepo and cucurbita maxima and the earthworms eisenia fetida and lumbricus terrestris.	two plant species, cucurbita pepo and cucurbita maxima, and two earthworm species, eisenia fetida and lumbricus terrestris, were exposed to soil and compost with equivalent p,p'-dde contamination. pollutant bioconcentration was equal in plant roots in both media, but translocation was higher in c. pepo. bioaccumulation by e. fetida was approximately 6- and 3-fold higher than that by l. terrestris in the soil and compost, respectively. for all species, p,p'-dde uptake was significantly greater fr ...	2007	17241722
in situ phytoextraction of polychlorinated biphenyl - (pcb)contaminated soil.	a pilot-scale field trial of phytoextraction of pcbs provides insight into the practical application of this technology, using the plant species cucurbita pepo ssp pepo cv. howden (pumpkin), carex normalis (sedge), and festuca arundinacea (tall fescue). this in situ trial took place at a historically contaminated field site, in soil contaminated with a mean concentration of 46 microg/g (range of 0.6 - 200 microg/g) total pcbs (aroclor 1254/1260). shoot bioaccumulation factors (where baf(shoot)=[ ...	2007	17258285
membrane associated qualitative differences in cell ultrastructure of chemically and high pressure cryofixed plant cells.	membrane contrast can sometimes be poor in biological samples after high pressure freezing (hpf) and freeze substitution (fs). the addition of water to the fs-medium has been shown to improve membrane contrast in animal tissue and yeast. in the present study we tested the effects of 1% and 5% water added to the fs-medium (2% osmium with 0.2% uranyl acetate in anhydrous acetone) on the quality and visibility of membranes in high pressure frozen leaf samples of cucurbita pepo l. plants and compare ...	2007	17270463
in vitro antioxidant properties of cucurbita pepo l. male and female flowers extracts.	total antioxidant capacities, 2,2-diphenyl-1-picrylhydrazyl (dpph.), hydroxyl (ho.), scavenging activities, and total phenolic values were determined in extracts of cucurbita pepo l. female and male flowers. powdered c. pepo l. samples were extracted in aqueous ethyl acetate (ea: w1, 17:3), ethanol (e), and water (w) by agitating in magnetic stirrer for 80 degrees c, 15 min and also by in aqueous ethyl acetate (ea: w2, 17:3) at 25 degrees c, 15 min. dpph., ho. scavenging capacities and total phe ...	2007	17308962
characterization of esterases from cucurbita pepo cv. "eskandrani".	two of the six esterases identified in cucurbita pepo cv. "eskandrani" were purified to homogeneity using two chromatography steps: anion exchange and gel filtration. the molecular weights of c. pepo esterases eic and eii were 50,000 +/- 1500 and 68,000 +/- 1900 da from gel filtration and 47,000 and 66,000 da from sds/page, respectively, suggesting a monomeric structure for both enzymes. esterases eic and eii had k(m) values of 1.22 and 1.56 mm and ph optima at 9.0 and 8.0, respectively. the sub ...	2008	17321740
ethnomedicines used in trinidad and tobago for reproductive problems.	throughout history women have tried to control or enhance their fertility using herbal remedies, with various levels of societal support. caribbean folk medicine has been influenced by european folk medicine, either through the early spanish and french settlers or through the continuous immigration of spanish-speaking peoples from venezuela. some folk uses are ancient and were documented by galen and pliny the elder.	2007	17362507
chlorophyllase is a rate-limiting enzyme in chlorophyll catabolism and is posttranslationally regulated.	chlorophyll is a central player in harvesting light energy for photosynthesis, yet the rate-limiting steps of chlorophyll catabolism and the regulation of the catabolic enzymes remain unresolved. to study the role and regulation of chlorophyllase (chlase), the first enzyme of the chlorophyll catabolic pathway, we expressed precursor and mature versions of citrus (citrus sinensis) chlase in two heterologous plant systems: (1) squash (cucurbita pepo) plants using a viral vector expression system; ...	2007	17369368
detection by rapd-pcr of polymorphism in populations of bemisia tabaci (genn.) collected on four host plants from iran.	the sweetpotato whitefly, bernisia tabaci (genn.) (hom: aleyrodidae) is a major pest of field crops, vegetables and ornamentals in iran. in this study, the infested leaves of cucumber (cucurnis sativus l.) zucchini (cucurbita pepo l.) eggplant (solanum melongena l.) and cotton (gossypium hirsutum l.) with whitefly nymphs and pupae were collected from iran, and were transferred to the laboratory. the newly emerged males and females of each population were released separately into a large cage set ...	2006	17385530
qualitative and quantitative differences in carotenoid composition among cucurbita moschata, cucurbita maxima, and cucurbita pepo.	squashes and pumpkins are important dietary sources of carotenoids worldwide. the carotenoid composition has been determined, but reported data have been highly variable, both qualitatively and quantitatively. in the present work, the carotenoid composition of squashes and pumpkins currently marketed in campinas, brazil, were determined by hplc-dad, complemented by hplc-ms for identification. cucurbita moschata 'menina brasileira' and c. moschata 'goianinha' had similar profiles, with beta-carot ...	2007	17444652
physiological and metabolic changes of cucurbita pepo leaves in response to zucchini yellow mosaic virus (zymv) infection and salicylic acid treatments.	the changes of some physiological and biochemical parameters in pumpkin (cucurbita pepo cv eskandarani) leaves associated with zucchini yellow mosaic virus (zymv) infection and the effect of exogenous application of salicylic acid (sa) were studied in this paper. in comparison to the untreated leaves, zymv infected leaves showed many symptoms, including severe mosaic, size reduction, stunting and deformation. results from analysis of physiological parameters indicated that viral infection and sa ...	2007	17466528