PMID(sorted ascending)
evidence about the catecholoxidase activity of the enzyme ascorbate oxidase extracted from cucurbita pepo medullosa.pure ascorbate oxidase (l-ascorbate:oxygen oxidoreductase, ec isolated from cucurbita pepo medullosa, which is known to be specific for ascorbic acid, shows a secondary catecholoxidase activity at approx. ph 6.7. this activity was tested against natural and synthetic compounds possessing a catechol-like structure. among natural compounds (+)-catechin furnishes the same complex oxidation mixture obtained with other oxidases. among synthetic compounds, 3,5-di-t-butylcatechol and 4-t-buty ...197720960
location of gluconeogenesis from phosphoenolpyruvate in cotyledons of cucurbita pepo.1. the aim of this work was to discover the location of the enzymes that convert phosphoenolpyruvate to fructose 6-phosphate during gluconeogenesis in fatty seeds. cotyledons of 5-day-old dark-grown seedlings of marrow (cucurbita pepo) were used as experimental material. 2. cotyledons were separated into palisade and mesophyll tissue. extracts of the two tissues had comparable activities of gluconeogenic enzymes. 3. extracts of cotyledons were fractionated by density gradient centrifugation to y ...1975164923
electron-paramagnetic-resonance studies of the mechanism of leaf nitrite reductase. signals from the iron-sulphur centre and haem under turnover conditions.low-temperature e.p.r. spectra are presented of nitrite reductase purified from leaves of vegetable marrow (cucurbita pepo). the oxidized enzyme showed a spectrum at g=6.86, 4.98 and 1.95 corresponding to high-spin fe(3+) in sirohaem, which disappeared slowly on treatment with nitrite. the midpoint potential of the sirohaem was estimated to be -120mv. on reduction with na(2)s(2)o(4) or na(2)s(2)o(4)+methyl viologen a spectrum at g=2.038, 1.944 and 1.922 was observed, due to a reduced iron-sulphu ...1978208505
identification of the regulatory steps in gluconeogenesis in cotyledons of cucurbita pepo.1. the aim of this work was to discover the steps at which the conversion of oxaloacetate to glucose 6-phosphate during gluconeogenesis is regulated in the cotyledons of 5-day-old seedlings of cucurbita pepo. 2. we estimated the maximum catalytic activities of all the enzymes in the above sequence and also the amounts of their substrates present in vivo. the results show that the reactions catalysed by fructose-1,6-bisphosphatase and phosphoenolpyruvate carboxykinase are the only ones in the seq ...1990208645
ascorbate oxidase from cucurbita pepo medullosa. new method of purification and reinvestigation of properties.1. ascorbate oxidase has been isolated from the green squash cucurbita pepo medullosa by a new purification method. furthermore a low-molecular-weight copper protein containing one type-1 copper/20000 mr could be separated during the purification of the oxidase. the six-step procedure developed improved the yield of ascorbate oxidase by a factor of 2.5. the method is well reproducible and a constant value of 8 cu (7.95 +/- 0.1/140000 mr) has been established. by ultracentrifugal and electrophore ...1996228938
mavicyanin, a blue copper protein from cucurbita pepo medullosa. purification and characterization.1. the copper protein mavicyanin has been isolated and purified from the green squash cucurbita pepo medullosa. 2. mavicyanin contains one type-1 copper/18000 mr, which can be characterized by: intense absorption maximum at 600 nm (epsilon = 5000 m-1 cm-1/cu, a280/a600 = 8.0 +/- 0.5, a600/a403 = 7.0 +/- 0.25, maximum of fluorescence emission at 335 nm. 3. in the oxidized state the copper of mavicyanin is 100% detectable by electron paramagnetic resonance (epr). computer simulation of the rhombic ...1979228939
a lectin from the exudate of the fruit of the vegetable marrow (cucurbita pepo) that has a specificity for beta-1,4-linked n-acetylglucosamine oligosaccharides.lectins are present in the exudate (presumably from the phloem) of the fruits of three species of the cucurbitaceae, namely vegetable marrow (cucurbita pepo), melon (cucumis melo) and cucumber (cucumis sativus). they are all strongly inhibited in their activities by chitin oligosaccharides, but only weakly by n-acetylglucosamine. glycopeptides from soya-bean agglutinin and fetuin are also strong inhibitors of cucurbita pepo lectin, indicating that it interacts with internal n-acetylglucosamine r ...1979534476
[interaction of ascorbate oxidase with inorganic anions].from the peelings of cucumber cucumis sativus and marrow squash cucurbita pepo var. giramontia highly purified ascorbate oxidase preparations were obtained. molecular weights, optical and epr spectra, total copper contents and different type copper contents of the both proteins were similar. the effects of nan3, kcn, i- and f- on the optical and epr spectra of the proteins were studied. the incubation of ascorbate oxidase with these anions lead to the partial reduction of the copper. the data ob ...1977588636
phosphoenolpyruvate carboxykinase and gluconeogenesis in cotyledons of cucurbita pepo.1. the aim of this work was to investigate the role of phosphoenolpyruvate carboxykinase (atp:oxaloacetate carboxy-lyase (transphosphorylating) ec in the conversion of fat to sugar by the cotyledons of seedlings of cucurbita pepo. 2. the enzyme was partially purified from the cotyledons of 5-day-old seedlings. the michaelis constants for oxaloacetate and atp were 56 and 119 micron, respectively. the decarboxylation reaction was optimum at ph 7.4. a range of intermediary metabolites did ...1978656445
[purification of peroxidases and separation of isoperoxidases from various plant species (author's transl)].the partial purification of peroxidase (ec. and separation of isoperoxidases by disc electrophoresis from cucurbita pepo l., phaseolus vulgaris l., cicer arietinum l. and hordeum, secale and triticum sp., have been studied. peroxidase from fruit of pumpkin and from 6-day-old coleoptiles of french bean and chick pea has been partially purified, 128-, 174-, and 140-fold, respectively. the apparent km at the optimum ph were: pumpkin (epicarp.), 2.7 x 10(-4) m; barley, common rye and wheat ...1977897327
autoradiographic and ultrastructural study of cucurbita pepo root cells during their growth and differentiation.cortex cells of the root meristem of cucurbita pepo (0.0-0.5 mm from the cap junction), in the 3-4, 5-6 and 7-8 mm segments above the root tip, and the cells of the first three layers of lateral part of root cap were the object of the present study. the volume of cortex cells increases more than 20 times in the 7-8 mm segment as compared with meristematic cells, and the volume of cytoplasm about sevenfold. the largest increment of the cytoplasmic volume occurs between 0.5-6.0 mm. in consecutive ...1976993066
[reaction of the seedlings of flowering plants to the effects of imino-di-(paradimethylaminophenyl)-methane-aurine-00].the influence of auramine-00 on seedlings of three species of plants - cucurbita pepo l., helianthus annuus l., zea mays l.-was investigated. auramine was added in agar-agar, on which the seedlings were growing. two concentrations of auramine were used--0.001%. besides the delay in growth, also the formation of local overgrowth of cotyledons and hypocotyl of c. pepo, the base of cotyledons of h. annuus and mezocotyl of z. mays were observed. the histological analysis has shown these swellings to ...19751166654
[response of flowering plant seedlings to the action of n-nitroso-n-methylurea].the influence of n-nitroso-n-methylurea on seedlings of two species of plants--cucurbita pepo l., helianthus annuus l.--was investigated. nmu was added in agar-agar, on which seedlings were growing. 0.02% concentration of nmu was used. in addition to the delay in growth and appearance of necrotic regions, also local overgrowth of cotyledons of c. pepo and overgrowth of hypocotyl and the base of cotyledons of h. annuus were observed. a histological analysis has shown that these swellings arise du ...19751210149
peroxidase as a developmental marker in plant tissue culture.peroxidase was studied as a developmental marker in pumpkin (cucurbita pepo l.) callus lines and horse-radish (armoracia lapathifolia gilib) transformants. embryogenic callus lines de grown on ms medium with 2.4-d and na-3 grown on medium with naa and adenine sulfate showed about a 20 times higher enzyme activity than the habituated non-embryogenic line z5b/t grown on medium without hormones. a rise in peroxidase activity indicated that somatic embryogenesis was triggered in a few habituated tis ...19911667582
treatment of benign prostatic hyperplasia with a randomised, double-blind study, the preparation curbicin, obtained from pumpkin seeds and dwarf palm plants (cucurbita pepo l. and sabal serrulata), was compared with a placebo in the treatment of symptoms caused by prostatic hyperplasia; 53 patients took part in the study, which was carried out over a 3-month period. urinary flow, micturition time, residual urine, frequency of micturition and a subjective assessment of the effect of treatment were all significantly improved in the treatmen ...19901702340
differential replication in zucchini squash of a cucumber mosaic virus satellite rna maps to rna 1 of the helper virus.cucumber mosaic virus (cmv) supports the replication and encapsidation of its satellite rna, both in solanaceous and cucurbit host plants; however, different strains of cmv support the replication of satellite rnas with different efficiency. in addition, replication of satellite rna is very efficient in solanaceous host plants and generally poor in cucurbit host plants. the wl1-satellite (wl1-sat) rna is an exception, and replicates to high levels in both solanaceous and curcubit host plants wit ...19911704659
the influence of plant growth regulators on callus induction in pumpkin (cucurbita pepo l.) hairy roots.following in vitro infection with agrobacterium rhizogenes wild strain (mannopine, 8196) and two a. tumefaciens transconjugant strains (c58c1 para4abc and c58c1 para4b) transformed (hairy) roots were induced in pumpkin (c. pepo l.) cotyledons. the presence of pri t-dna in pumpkin long-term hairy root cultures was determined by southern hybridization. the influence of plant growth regulators on callus induction in root explants from hairy root lines, which differed mutually in morphology and grow ...19911814408
two genes encoding 1-aminocyclopropane-1-carboxylate synthase in zucchini (cucurbita pepo) are clustered and similar but differentially regulated.a 17-kilobase (kb) region of the zucchini (cucurbita pepo) genome has been sequenced and contains two genes, cp-acc1a and cp-acc1b, encoding 1-aminocyclopropane-1-carboxylate synthase (acc synthase; s-adenosyl-l-methionine methylthioadenosine-lyase, ec the genes are transcribed convergently and are separated by a 5.7-kb intergenic region. their coding regions are interrupted by four introns located in identical positions. while the dna identity in their coding regions is 97%, their 5' ...19911871117
infectious in vitro rna transcripts derived from cloned cdna of the cucurbit potyvirus, zucchini yellow mosaic virus.a full-length cdna clone of the rna genome of the cucurbit potyvirus zucchini yellow mosaic virus (zymv) was constructed downstream from a bacteriophage t7 rna polymerase promoter. a single extra guanosine residue not present in zymv rna was added to the 5' and 3' ends. capped (m7gpppg) zymv rna transcripts were infectious in 10 of 91 cucurbita pepo test plants; uncapped rna transcripts were not infectious. the appearance of symptoms in plants inoculated with the infectious transcript was delaye ...19911940860
gc balance in the internal transcribed spacers its 1 and its 2 of nuclear ribosomal rna genes.the internal transcribed spacer (its) 1 and 2, the 5.8s rrna gene, and adjacent 18s rrna and 25s rrna coding regions of two cucurbitaceae (cucurbita pepo, zucchini, its 1: 187 bp, and its 2: 252 bp in length, and cucumis sativus, cucumber, its 1: 229 bp, and its 2: 245 bp in length) have been sequenced. the evolutionary pattern shown by the itss of these plants is different from that found in vertebrates. deletions, insertions, and base substitutions have occurred in both spacers; however, it is ...19902107331
a cucumber mount for the preparation of histological sections of the small intestine of rodents.a method for orientation of formaldehyde-fixed intestinal tissue from mink puppies (mustela vision) is described. sections of the intestine were glued to dehydrated slices of cucumber (cucumis sativus l. or cucurbita pepo l.) with egg albumin. this cucumber-intestine unit was processed in a routine way. embedding in paraplast was facilitated thereby ensuring a correct orientation of the tissue.19902191529
restriction site and genetic map of cucurbita pepo chloroplast dna.a detailed restriction map of squash chloroplast dna (cpdna) was constructed with five restriction endonucleases, sali, pvuii, bgli, sacii, and psti. the cleavage sites were mapped by sequential digestion of cpdna using low-gelling temperature agarose. the restriction map shows that squash cpdna is an approximately 153 kilobase (kb) circle with a large inverted repeat sequence of 23.3 kb, separated by a large (83.7 kb) and a small (22.7 kb) single copy region. genes for a number of chloroplast p ...19902249258
computer analysis of phytochrome sequences from five species: implications for the mechanism of action.the amino acid sequences of phytochrome from avena sativa, oryza sativa, curcurbita pepo, pisum sativum and arabidopsis thaliana have been analyzed with a variety of computer programs, with a view to identifying areas of the protein which contribute to the properties of this photoreceptor. a region at the c-terminus has been shown to be amphiphilic, and by analogy with surface-seeking peptides, may be responsible for interaction of phytochrome with lipid bilayers. possible targeting sequences in ...20092291773
phospholipid-stimulated protein kinase in membrane fractions from zucchini (cucurbita pepo l.) hypocotyls, catalytic properties of a platelet-activating factor (paf)-activated protein kinase were investigated. in the presence of [ethylenebis(oxyethylenenitrilo)]tetraacetic acid, phosphorylation of a 55-kda membrane polypeptide and, to a lesser extent, several others, including a 120-kda polypeptide, was stimulated by paf. the phosphorylation of the 55-kda polypeptide was used for quantification of the paf-stimulated protein kinase. s ...19892530218
kinetic features of ascorbic acid oxidase after partial means of specific exoglycosidases, sugars have been removed under non-denaturing conditions from ascorbic acid oxidase (aao), different deglycation schedules being followed. our results indicate that deglycation clearly affects the kinetic features of aao, leading to an increase of 'affinity' and 'catalytic ability' of the enzymic forms so generated. a better exposure of the catalytic-site residues could be supposed to occur upon treatment with exoglycosidases. this is supported by the three- ...19892604732
species and genus specificity of the intergenic spacer (igs) in the ribosomal rna genes of cucurbitaceae.the use of intergenic spacer (igs) fragments of plant ribosomal dna (rdna) for the differentiation between genera and species is tested by cross-hybridization experiments with different igs probes of two cucurbitaceae, cucurbita pepo (zucchini) and cucumis sativus (cucumber). hybridization with cloned fragments of different parts of the igs of ribosomal dna exhibit a different degree of conservation within and between the cucurbitaceae genera. in general, cucurbita species seem to be closer rela ...20092631730
gtp causes calcium release from a plant microsomal fraction.studies on a variety of animal cell types have revealed a gtp-specific calcium-releasing mechanism in a non-mitochondrial, microsomal fraction. here we report that gtp also induces rapid release of calcium from a zucchini (cucurbita pepo l.) hypocotyl microsomal fraction. maximal release occurs at 50 microm, and half-maximal release at 8 microm gtp. gtp is highly specific in its effect, and may not be replaced by utp, atp, ctp, ttp, gmp, or by non-hydrolysable analogues of gtp. reuptake of calci ...19892701802
a rapid response to a plant hormone: auxin stimulates phospholipase a2 in vivo and in vitro.addition of the active auxins indole-3-acetic acid, 2,4-dichlorophenoxyacetic acid or alpha-naphthylacetic acid to cultured soybean (glycine max l.) cells prelabeled with ethanolamine or choline increased the radioactivity in the lysophosphatidylethanolamine (lpe) or lysophosphatidylcholine (lpc) pool within 5 min. the inactive auxin analogue, beta-naphthylacetic acid, was inactive in this response. in membranes prelabeled in vivo, either with ethanolamine or choline, and subsequently isolated f ...19892775252
the reaction of nitric oxide with copper proteins and the photodissociation of copper-no complexes.the reactivity with nitric oxide was investigated for a number of type-1, type-2 and type-3 copper proteins azurin from pseudomonas aeruginosa (type-1 copper); bovine superoxide dismutase, diamine oxidase from pig kidney and galactose oxidase from dactylium dendroides (type-2 copper); haemocyanin from helix pomatia (type-3 copper); the blue oxidases ceruloplasmin from pig serum, and ascorbate oxidase from cucurbita pepo medullosa. type-1 copper formed complexes with no in the oxidised state, whi ...19872822126
azide-binding studies reveal type 3 copper heterogeneity in ascorbate oxidase from the green zucchini squash (cucurbita pepo).titration of native ascorbate oxidase from green zucchini squash (cucurbita pepo) with azide in 0.1 m-phosphate buffer, ph 6.8, exhibits a biphasic spectral behaviour. binding of the anion with 'high affinity' (k greater than 5000 m-1) produces a broad increase of absorption in the 400-500 nm region (delta epsilon approximately 1000 and c.d. activity in the 300-450 nm region, whereas azide binding with 'low affinity' (k approximately 100 m-1) is characterized by an intense absorption b ...19882840893
active auxin uptake by zucchini membrane vesicles: quantitation using esr volume and delta ph determinations.closed and ph-tight membrane vesicles prepared from hypocotyls of 5-day-old dark-grown seedlings of cucurbita pepo accumulate the plant growth hormone indole-3-acetic acid along an imposed proton gradient (ph low outside, high inside). the use of electron paramagnetic spin probes permitted quantitation both of apparent vesicle volume and magnitude of the ph gradient. under the experimental conditions used, hormone accumulation was at minimum 20-fold, a value 4 times larger than what one would pr ...19852995970
specific guanine nucleotide binding by membranes from cucurbita pepo seedlings.a microsomal membrane preparation from hypocotyls of dark-grown cucurbita pepo l. (zucchini) seedlings contains specific high-affinity binding sites for the non-hydrolyzable gtp analog guanosine 5'-[gamma-thio]triphosphate (gtp-gamma-s). both the binding affinity and the pattern of binding specificity for gtp and guanine nucleoside triphosphate analogs are shared with the more thoroughly characterized animal g-proteins that are known to be involved in signal transduction. the sensitivity of gtp- ...19883052446
presence of guanine nucleotide-binding proteins in a plant hypocotyl microsomal fraction.the presence of specific guanine nucleotide-binding proteins in a zucchini (cucurbita pepo l.) hypocotyl microsomal fraction was investigated. polypeptides were separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis and transferred to nitrocellulose. incubation of nitrocellulose blots with [alpha-32p]gtp and [gamma-32p]gtp indicated the presence of four specific and distinct gtp-binding proteins with molecular masses of approx. 23.4 kda, 24.8 kda, 26.6 kda and 28.5 kda. binding ...19883124845
characterization of the tmv encapsidation initiation site on 18s rrna.tobacco mosaic virus capsid protein oligomers react with and encapsidate 18s rrna from both plant and mammalian sources in vitro. the site (ei) in 18s rrna which reacts with capsid protein to initiate the packaging reaction has been localized and partially characterized by testing the ability of transcripts from different regions of a cloned cucurbita pepo rdna repeat unit to become encapsidated. the 18s rrna ei is found to react more slowly with capsid protein than does the functional virion ei ...19883412887
protein inhibitors of trypsin from the seeds of cucurbitaceae trypsin inhibitors were isolated from the seeds of cucurbitaceae plants: two from cucumber (cucumis sativus) and red bryony (bryonia diotica) and one from figleaf gourd (cucurbita ficifolia), spaghetti squash (cucurbita pepo var. (vegetable spaghetti) and water melon (citrullus vulgaris). the inhibitors were purified by fractionation with ammonium sulphate, followed by ion-exchange chromatography and affinity chromatography using immobilized trypsin or anhydro-trypsin. the homogeneous inhi ...20073450102
release of ca2+ from plant hypocotyl microsomes by inositol-1,4,5-trisphosphate.the effect of inositol-1,4,5-trisphosphate on ca2+ release from microsomes isolated from dark-grown zucchini (cucurbita pepo l.) hypocotyls was studied. up to 30% of the ca2+ taken up by the microsomes in the presence of 2mm atp, was released by mumolar concentrations of inositol-1,4, 5-trisphosphate. this release was very rapid (less than 0.5 min) and was followed by a slower re-uptake of ca2+. the microsomal levels of ca2+ previously attained were not re-established within 5 min. external conc ...19853875346
purification and characterization of the trypsin inhibitor from cucurbita pepo var. patissonina fruits.three trypsin inhibitor fractions were found in white bush fruits (cucurbita pepo l. var. patissonina). one of them, cppti-fiii, was purified to homogeneity by means of affinity and ion exchange chromatography. it is a cysteine-poor protein with an approximate mr of 21 000. the inhibitor contains arginine at position p1 of the reactive site and inhibits bovine trypsin, hog pancreatic kallikrein and subtilisin. this inhibitor differs from the inhibitors of white bush dormant seeds, cppti-i and cp ...19853933527
the serine proteinase inhibitor from summer squash (cucurbita pepo): some structural features, stability and proteolytic degradation.the serine proteinase inhibitor from summer squash seeds (cpti-ii) with mr of about 3250 contains three disulphide bridges and is unusually resistant to denaturing agents (e.g. 10% trichloroacetic acid at about 100 degrees c), thermolysin and proteinase v8 from staphylococcus aureus. the inhibitor is digested by pepsin; the digestion of the virgin form proceeds more rapidly than when the peptide bond of the reactive site is broken. the inhibitor is not specifically reduced by sodium borohydride ...19853938587
isolation of two trypsin inhibitors from resting seeds of the white bush (cucurbita pepo var. patissonina) and their properties.two trypsin inhibitors, cppti-i and cppti-ii of mr 3 250 and 7 850, respectively, were isolated from resting white bush seeds. both inhibitors are cysteine-rich proteins. in addition to trypsin, they inhibit a trypsin-like enzyme isolated from streptomyces griseus proteinase but they do not act on chymotrypsin, kallikrein or subtilopeptidase a. the isolated inhibitors contain a lysine residue in position p1 of the reactive site.19853938588
nitrite and hydroxylamine reduction in higher plants. fractionation, electron donor and substrate specificity of leaf enzymes, principally from vegetable marrow (cucurbita pepo l.).nitrite reductase was purified between 760- and 1300-fold from vegetable marrow (cucurbita pepo l.) and residual hydroxylamine reductase activity was low or negligible by comparison. with ferredoxin as electron donor, nitrite loss and ammonia formation at ph7.5 were stoicheiometrically equivalent. crude nitrite reductase preparations showed negligible activity with nadph as electron donor maintained in the reduced state by glucose 6-phosphate, whereas by comparison, activity was high when either ...19704395427
the amino acid sequence of plastocyanin from cucurbita pepo l. (vegetable marrow).the amino acid sequence of plastocyanin from marrow was determined. it consists of a single polypeptide chain of mol.wt. 10284 containing 99 amino acid residues. the sequence was determined by using a beckman 890c automatic sequencer and by dansyl-phenyl isothiocyanate analysis of peptides obtained by the enzymic digestion of purified cnbr fragments. the sequence is in good agreement with the amino acid composition, except that fewer residues of glutamic acid were found in the sequence than were ...19744462555
fractionation of the proteins of plant microbodies.1. glyoxysomes and peroxisomes have been isolated from dark- and light-grown seedlings of pumpkin (cucurbita pepo) by sucrose-density-gradient centrifugation. 2. pumpkin microbodies and castor-bean (ricinus communis) glyoxysomes may be fractionated, by a combination of osmotic shock and treatment with kcl, into three distinct groups of proteins: readily soluble (matrix enzymes), solubilized in the presence of kcl (membrane-bound enzymes) and relatively insoluble (membrane ;ghost' proteins). 3. s ...19744468822
characteristics of the rapidly reassociating deoxyribonucleic acid of cucurbita pepo l. and the sequences complementary to ribosomal and transfer ribonucleic acids. 19734711479
[gel filtration of ascorbic acid oxidase in cucumbers (cucurbita pepo)]. 19674971157
[localization of acid deoxyribonuclease in the radicular meristem of the pumpkin (cucurbita pepo l. cucurbitaceae)]. 19694989892
[localization of thiolacetic esterase in the radicular meristem of the pumpkin (cucurbita pepo l cucurbitaceae)]. 19694989954
the purification and some properties of a protein causing gelling in phloem sieve tube exudate from cucurbita pepo. 19725022434
the isolation and some properties of a factor causing gelling in the phloem sieve-tube exudate of the squash plant (cucurbita pepo). 19715127345
plastocyanin from cucurbita pepo l. 19715158501
respiratory metabolism in virus-infected capsicum annuum l. & cucurbita pepo l. 19695359094
possible sites in nitrite reductase and hydroxylamine reductases from vegetable marrow (cucurbita pepo l). 19705420040
[investigation of salt-soluble proteins of pumpkin seeds (cucurbita pepo l.) by a column gradient extraction method]. 20095870889
a protein-protochlorophyll complex obtained from inner seed coats of cucurbita pepo. the resolution of its two pigment groups into true protochlorophyll and a pigment related to bacterial protochlorophyll.1. the inner seed coats of cucurbita pepo were extracted with aqueous acetone and found to contain pigments with spectra similar to that of protochlorophyll. 2. when the fruits of c. pepo were stored the amount of protochlorophyll-like material in the inner seed coats increased and a form of protochlorophyll absorbing at longer wavelength was apparently formed. 3. the pigment was resolved into two forms of protochlorophyll by chromatography on sugar columns. one form with absorption maxima in et ...19665971776
third-harmonic detection of electron-paramagnetic-resonance spectra: resolution of the hyperfine splitting in nitrosylated nitrite reductase from vegetable marrow (cucurbita pepo). 19806256243
use of triton wr-1339 in cytochemical and biochemical characterization of phytolysosomes.a new cytochemical assay of acid phosphatase activity employing triton wr-1339, a non-ionic detergent, has been used to demonstrate the probable origin of the phytolysosome system and of the organization of the golgi complex in the root meristem, cucurbita pepo. the results were controlled biochemically by differential and linear density gradient ultracentrifugation of extracts from normal and triton-treated roots. the various fractions were characterized by electron microscopy or by assay of ma ...19826284682
peptide hydrolase activities in seedlings and hormone-treated cotyledons of pumpkin (cucurbita pepo).enzymes hydrolyzing gly-ala-, met-met- and pro-4-phenylazo-phenylamides, and n-benzoyl-l-arginine-4-nitroanilide have been identified in germinating seeds and cotyledons of pumpkin (cucurbita pepo). the enzyme activities per cotyledon increase markedly during the germination process, but the proportion of enhancement depends on the type of enzyme species. the increase in enzyme activities is due to de novo synthesis as shown by cycloheximide treatment and is influenced by phytohormones (cytokini ...19836360167
trypsin inhibitors in summer squash (cucurbita pepo) seeds. isolation, purification and partial characterization of three inhibitors.three trypsin inhibitors were isolated from summer squash (cucurbita pepo) seeds and purified to homogeneity by fractionation with ammonium sulphate and methanol, ion-exchange chromatography and gel filtration. all three inhibitors have lysine at their active site. two of them (ii, iv) show the same isoelectric point (at ph 5.6), amino acid composition and molecular mass (3259). the third inhibitor (iii) of molecular mass of 3654 and isoelectric point at 4.9 has additionally one histidine residu ...19846524213
a reinvestigation on the quaternary structure of ascorbate oxidase from cucurbita pepo medullosa.the optical properties, copper content, catalytic activity and quaternary structure of many preparations of ascorbate oxidase purified with two different methods were examined. fresh samples appeared identical and were characterized by optical ratios a280/a610 = 25 +/- 1 and a330/a610 = 0.8 +/- 0.05, by specific activity toward ascorbate of 3.48 +/- 0.05 mol g-1 min-1 and by a copper content of 8 +/- 0.3 mol/145 000 mr. the enzyme is composed of two non-covalently linked subunits of slightly dif ...19836646110
[essential amino acids of pumpkin-seed pie (cucurbita pepo, l.) for chickens]. 19816800320
preparation and characteristics of trypsin inhibitors from the seeds of squash (cucurbita maxima) and zucchini (cucurbita pepo var. giromontia).one trypsin inhibitor (i) from zucchini seeds and two (i and iii) from squash seeds were isolated by ammonium sulphate salting out and chromatography on sp-sephadex c-25 and immobilized trypsin. the inhibitors have the same molecular mass, about 3300, contain 29 amino acids, have three disulphide bridges, and lack thr, phe and trp. the isoelectric point of the zucchini inhibitor i and squash inhibitor i is of 5.6, whereas that of the squash inhibitor iii--of 8.3. arg is the n-terminal amino acid ...19836868907
kinetics of leaf nitrite reductase with methyl viologen and ferredoxin under controlled redox conditions.some factors that influence the activity of nitrite reductase (ec were investigated, the enzyme from curcurbita pepo (vegetable marrow) being used. the activity with ferredoxin or methyl viologen as electron donor was inhibited by certain salts, including nacl. the steady-state kinetic parameters measured in a commonly used open-tube (aerobic) system were compared with a closed-cell (anaerobic) system in which the redox potential, and thus the concentrations of oxidized and reduced dono ...19827126179
host susceptibility of the papaya mosaic virus in sri lanka.75 plant species from 11 families were tested in sri lanka for their susceptibility to transferring the papaya mosaic virus. after inoculation with this virus, six species, cucurbita pepo, cucumis sativus, nicotiana tabacum, chenopodium amaranticolor, gomphrena globosa and lycopersicum esculentum, developed such symptoms, and after re-isolation from the host plant the virus again infected papaya plants. thus these species are possible alternate hosts of papaya mosaic virus in sri lanka.19817342959
individual quantitative rdna variation in three species of the cucurbitaceae family.individual quantitative variation in rdna content within three species of the cucurbitaceae family has been studied by rrna/dna filter hybridization experiments. the results showed a 2.3-fold range of variation in the number of ribosomal cistrons per diploid cell in an ecballium elaterium natural population. this range of variation is compared with the smaller range observed in three cucumis sativus and in two cucurbita pepo varieties obtained as f1 hybrids between pure lines.19807437009
galactinol synthase from kidney bean cotyledon and zucchini leaf. purification and n-terminal sequences.galactinol synthase (gs) was purified 1591-fold with a 3.9% recovery from the cotyledon of kidney bean (phaseolus vulgaris) by a novel scheme consisting of ammonium sulfate fractionation followed by diethylaminoethyl, affi-gel blue, and udp-hexanolamine affinity chromatography. the purified enzyme had a specific activity of 8.75 mumol mg-1 min-1, a ph optimum of 7.0, and requirements for manganese ion and dtt. the enzyme exhibited a km = 0.4 mm for udp-galactose and a km = 4.5 mm for myo-inosito ...19957480343
synthesis, cloning and expression in escherichia coli of the gene coding for the trypsin inhibitor from cucurbita pepo.a chemically synthesized gene coding for the serine proteinase inhibitor cpti ii was cloned in e. coli and its expression was investigated in cytoplasmic and secretion systems. under all conditions investigated the biologically active form of the inhibitor was found only in the latter system, although the yield was rather low.19957653151
stability of japanese-lacquer-tree (rhus vernicifera) laccase to thermal and chemical denaturation: comparison with ascorbate oxidase.the thermal denaturation of laccase from the japanese lacquer tree (rhus vernicifera) was studied by differential scanning calorimetry. the endotherms of holo-laccase, type 2-cu-depleted laccase and apo-laccase were deconvoluted into two independent two-state transitions, providing evidence for a domain structure of the protein. the correlation of the two transitions with the bleaching of copper optical bands and the decrease of the transitions' enthalpy on cu removal show that the process invol ...19957702562
pattern and degree of methylation in ribosomal rna genes of cucurbita pepo l.methylation with respect to its degree and distribution throughout the 18s, 5.8s and 25s rrna gene clusters (rdna) and within single rdna repeats in seedlings of the higher plant cucurbita pepo l. (zucchini) was investigated. in this plant, which is characterized by several thousand repeats, at least 70% are completely or nearly completely methylated in cpgs and to a lower degree in cpnpgs. detailed methylation analysis revealed that a fraction of about 3-4% of all repeats is hypomethylated near ...19947811974
characterization of the p1 protein and coding region of the zucchini yellow mosaic virus.the nucleotide sequence of the 5'-terminal p1 coding region of an aphid-transmissible isolate of zucchini yellow mosaic virus (zymv; strain fl/at), a mild isolate (strain md) and a severe isolate (strain sv), all from florida, were compared with two other zymv isolates. the zymv md and sv isolates and an isolate from california (zymv ca) had 95-98% sequence similarities to fl/at, whereas an isolate from reunion island (zymv ru) had a 60% sequence similarity to fl/at. zymv md had an 18 nucleotide ...19957844540
characterization and sequencing of cdna clone encoding the phloem protein pp2 of cucurbita n-terminal amino acid sequencing of the phloem protein 2 (pp2) from 3-month old cucurbita pepo l. (pumpkin), purified by sds-page and blotted onto pvdf membrane, showed that the protein had a blocked n-terminus. however, after in situ cleavage of the polypeptide in a gel slice by cyanogen bromide, 75 residues of sequences on two cyanogen bromide fragments were determined. an oligonucleotide probe based on this amino acid sequence was used to screen a cdna library, constructed from mrna of ...19948111014
exhaustive removal of n-glycans from ascorbate oxidase: effect on the enzymatic activity and immunoreactivity.purified ascorbate oxidase from cucurbita pepo medullosa has been subjected to enzymatic deglycosylation using peptide n-glycosidase f. experimental conditions were chosen to obtain efficiently deglycosylated and active ascorbate oxidase: in particular, three different detergent solutions were added separately to the incubation mixtures prior to the peptide n-glycosidase f. the detergent solution made of 0.1% (w/v) sodium dodecyl sulphate + 0.5% (v/v) nonidet p-40 proved to be the only one effec ...19938130388
[main carotenoids in pressed seeds (cucurbitae semen) of oil pumpkin (cucurbita pepo convar. pepo var. styriaca)].various use of the oil-pumpkin offers reason for the phytochemical analysis of seed-meal's carotene pigments. column chromatography was performed on the adsorbents mgo, celite and caco3 with hexane and benzene as eluents. hplc separation of different pigments was carried out on a 6 microns reverse phase packing with a ternary gradient elution method using a diode-array detector. the main components of the press-residue were lutein [3,3'-dihydroxy-alpha-carotene = (3r,3'r,6'r)-beta,epsilon-carote ...19938249609
vapor heat treatment for zucchini infested with bactrocera cucumis (diptera: tephritidae).zucchini, cucurbita pepo l., infested with 22-h-old eggs of bactrocera cucumis (french) were completely disinfested using a vapor heat treatment of 45 degrees c with > 94% rh for 30 min. an estimated total of 178,219 eggs (22 h old) was treated without survivors, thus achieving probit 9 mortality. twenty-two-h-old eggs were significantly more tolerant of heat than first, second, or third instars. all treatments were done in a sanshu model ehk-1000b vapor heat treatment system.19938432953
molecular evolution of the intergenic spacer in the nuclear ribosomal rna genes of cucurbitaceae.the intergenic spacer (igs) of a 10-kbp repeat (clone prz7d) of the nuclear 18s, 5.8s, and 25s ribosomal rna genes of cucurbita pepo (zucchini) was sequenced and compared to the igs sequences of two other cucurbitaceae, curcurbita maxima (squash), and cucumis sativus (cucumber). the nucleotide sequence and the structural organization of the igs of c. pepo and c. maxima are rather similar (between 75 and 100% sequence similarity depending on the region compared). the igs are mainly composed of th ...19938433383
transcriptional regulation of hydroxypyruvate reductase gene expression by cytokinin in etiolated pumpkin understand the mechanisms by which the expression of a specific gene is modulated by cytokinin, the regulation of hydroxypyruvate reductase (hpr) transcript levels by n6-benzyladenine (ba) in etiolated pumpkin (cucurbita pepo l. cv. halloween) cotyledons was investigated. a pumpkin hpr cdna was generated by reverse transcriptase-polymerase chain reaction and its nucleotide sequence was determined. an antisense hpr rna was prepared for rnase protection analysis of hpr-mrna expression patterns ...19968580766
identification of the proteins responsible for sar dna binding in nuclear matrix of cucurbita pepo.the nuclear matrices from white bush (cucurbita pepo var. patisonina) cell nuclei have been isolated using three methods: i, standard procedure involving extraction of cell nuclei with 2 m nacl and 1% triton x-100; ii, the same with pre-treatment of cell nuclei with 0.5 mm cuso4 (stabilisation step); and iii, method with extraction by lithium diiodosalicylate (lis), and compared the polypeptide pattern. the isolated matrices specifically bind sar dna derived from human beta-interferon gene in th ...19958588459
immunoglobulins anti-endonuclease 32 kda from cucurbita pepo var patissonina affect process of dna synthesis.immunoglobulins anti-endonuclease 32 kda inhibit dna synthesis. we observed that low concentrations of iggs (about 50 micrograms igg per 1 x 10(6) cell nuclei) temporary inhibit dna synthesis. this inhibition concerns only the synthesis of dna bound to the nuclear matrix (associated with isolated nuclear matrix). preincubation of cell nuclei of white bush with igg generates longer dna fragments than in controls. involvement of the 32 kda endonuclease or an endonuclease-65 kda protein complex fro ...19958588460
variability of vitamin e content in pumpkin seeds (cucurbita pepo l.).pumpkin (cucurbita pepo l.) seed oil is a common salad oil which is produced in the southern parts of austria, slovenia and hungary. it is dark green and has a high content of free fatty acids. due to its colour, the oil cannot be used for cooking. the content of vitamin e, especially gamma-tocopherol, is very high. the oil content of the pumpkin seed is about 50%. the seed itself can be eaten. therefore a pumpkin variety with high vitamin e content is desirable. the aim of this work was to find ...19968638429
purification and characterization of assimilatory nitrite reductase from candida utilis.nitrate assimilation in many plants, algae, yeasts and bacteria is mediated by two enzymes, nitrate reductase (ec and nitrite reductase (ec they catalyse the stepwise reduction of nitrate to nitrite and nitrite to ammonia respectively. the nitrite reductase from an industrially important yeast, candida utilis, has been purified to homogeneity. purified nitrite reductase is a heterodimer and the molecular masses of the two subunits are 58 and 66 kda. the native enzyme exhibits ...19968694757
degradation pattern of photosystem ii reaction center protein d1 in intact leaves. the major photoinhibition-induced cleavage site in d1 polypeptide is located amino terminally of the de loop.photoinhibition-induced degradation of the d1 protein of the photosystem ii reaction center was studied in intact pumpkin (cucurbita pepo l.) leaves. photoinhibition was observed to cause the cleavage of the d1 protein at two distinct sites. the main cleavage generated an 18-kd n-terminal and a 20-kd c-terminal degradation fragment of the d1 protein. this cleavage site was mapped to be located clearly n terminally of the de loop. the other, less-frequent cleavage occurred at the de loop and prod ...19968756500
study of the three-dimensional images of potyvirus-induced cytoplasmic inclusions using confocal laser scanning microscopy.the three-dimensional structure of the cytoplasmic inclusions in leaves of cucurbita pepo l. infected with a singapore isolate of zucchini yellow mosaic virus (zymv-s) was studied using a confocal laser scanning microscope. the cytoplasmic inclusions were found to be linear filamentous structures that varied in thickness and length. the formation of the inclusions probably begins when finely-pointed linear filamentous structures aggregate into bundles forming flat parallel arrays, or diverge int ...19968844619
variability of fatty acid content in pumpkin seeds (cucurbita pepo l.).pumpkin (cucurbita pepo l.) seed oil is a common salad oil which is produced in slovenia, hungary and the southern parts of austria. it is dark green and has a high content of free fatty acids. the seed itself can be eaten. due to its colour and the foam formation, the oil cannot be used for cooking. the content of vitamin e, especially gamma-tocopherol, is very high. the oil content of the pumpkin seed is about 50%. the variability in the oil content is very high resulting from a broad genetic ...19968873459
purification, characterization and subcellular localization of a type-1 ribosome-inactivating protein from the sarcocarp of cucurbita pepo.the flesh of the fruit of cucurbita pepo contains a type-1 ribosome-inactivating protein (rip), which we named pepocin. pepocin was purified to apparent homogeneity by acid fractionation, ion-exchange chromatography and adsorption chromatography. the protein was found to have a molecular mass of 26 kda and a pi of about 9.9. it does not contain glycosidic linkages. the protein inhibits protein synthesis in a rabbit-reticulocyte lysate with an ic50 (concentration causing 50% inhibition) of 15.4 p ...19969022685
protein transport into higher plant peroxisomes. in vitro import assay provides evidence for receptor involvement.peroxisome biogenesis requires that proteins be transported from their site of synthesis in the cytoplasm to their final location in the peroxisome matrix or membrane. glyoxysomes are a class of peroxisomes found primarily in germinating seedlings and are involved in mobilizing fatty acids via the glyoxylate cycle and the beta-oxidation pathway. we have used an in vitro assay to study the mechanism(s) of import of proteins into glyoxysomes. results from this assay indicate that the transport pro ...19979112774
nucleotide triphosphates are required for the transport of glycolate oxidase into peroxisomes.all peroxisomal proteins are nuclear encoded, synthesized on free cytosolic ribosomes, and posttranslationally targeted to the organelle. we have used an in vitro assay to reconstitute protein import into pumpkin (cucurbita pepo) glyoxysomes, a class of peroxisome found in the cotyledons of oilseed plants, to study the mechanisms involved in protein transport across peroxisome membranes. results indicate that atp hydrolysis is required for protein import into peroxisomes; nonhydrolyzable analogs ...19989449847
the uptake of radionuclides by beans, squash, and corn growing in contaminated alluvial soils at los alamos national laboratory.pinto beans (phaselous vulgaris), sweet corn (zea mays), and zucchini squash (cucurbita pepo) were grown in a field pot study using alluvial floodplain soils contaminated with various radionuclides within los alamos canyon (lac) at los alamos national laboratory, new mexico. soils as well as washed edible (fruit) and nonedible (stems and leaves) crop tissues were analyzed for tritium (3h), cesium (137cs), strontium (90sr), plutonium (238pu and 239,240pu), americium (241am), and total uranium (to ...19989491570
structures of the n-linked carbohydrate of ascorbic acid oxidase from zucchini.the n-glycan moiety of ascorbic acid oxidase from zucchini (cucurbita pepo) has been described to be a core-pentasaccharide with a xylose [d'andrea et al. (1988) glycoconjugate j 5:151-7]. ascorbic acid oxidase is sometimes used to characterize antibodies directed against carbohydrate determinants on plant glycoproteins. to prevent misinterpretations of immunological data, the structure of the n-glycan of ascorbic acid oxidase has been reinvestigated. the oligosaccharides were released by almond ...19989530960
characterization of cucumber mosaic virus. v. cell-to-cell movement requires capsid protein but not ascertain the importance of amino-terminal proximal capsid protein (cp) sequences in cel-to-cell movement, virion formation, and stabilization, two cp mutants of cucumber mosaic virus (cmv) were generated by deletion of sequences encoding cp amino acids 15-40 (delta sal-nru) or 26-40 (delta sac-nru). wildtype cmv and cmv containing delta sac-nru could infect systemically four host species, although symptoms induced by the two viruses usually were different cmv containing delta sal-nru could o ...19989657941
characterization of spermidine binding to solubilized plasma membrane proteins from zucchini hypocotylsin this work [14c]spermidine binding to total proteins solubilized from plasma membrane purified from zucchini (cucurbita pepo l.) hypocotyls was investigated. proteins were solubilized using octyl glucoside as a detergent. specific polyamine binding was thermolabile, reversible, ph dependent with an optimum at ph 8.0, and had a kd value of 5 &mgr;m, as determined by glass-fiber-filter assays. sephadex g-25 m gel-filtration assays confirmed the presence of a spermidine-protein(s) complex with a ...19989662539
effect of tunicamycin on the activity and immunoreactivity of ascorbate oxidase (cucurbita pepo medullosa) expressed in cultured green zucchini cells.ascorbate oxidase activity and immunoreactivity were evaluated in crude tissue extracts obtained from callus cell cultures induced by green zucchini sarcocarp and grown in the presence of tunicamycin, a powerful n-glycosylation inhibitor. tunicamycin at 2 or 4 microg ml(-1) blocked cell growth within a couple of weeks, although a sustained cell viability was observed in the same period. a significant inhibition of total protein synthesis was observed at 10 and 15 days of culture time, with a dec ...19989870353
cloning, expression and n-terminal myristoylation of cpcpk1, a calcium-dependent protein kinase from zucchini (cucurbita pepo l.).we have isolated a full-length cdna clone (cpcdpk1) encoding a calcium-dependent protein kinase (cdpk) gene from zucchini (cucurbita pepo l.). the predicted amino acid sequence of the cdna shows a remarkably high degree of similarity to members of the cdpk gene family from arabidopsis thaliana, especially atcpk1 and atcpk2. northern analysis of steady-state mrna levels for cpcpk1 in etiolated and light-grown zucchini seedlings shows that the transcript is most abundant in etiolated hypocotyls an ...199910080688
high-resolution structures of three new trypsin-squash-inhibitor complexes: a detailed comparison with other trypsins and their anionic trypsin from atlantic salmon and bovine trypsin have been complexed with the squash-seed inhibitors, cmti-i (cucurbita maxima trypsin inhibitor i, p1 arg) and cpti-ii (cucurbita pepo trypsin inhibitor ii, p1 lys). the crystal structures of three such complexes have been determined to 1.5-1.8 a resolution and refined to crystallographic r factors ranging from 17.6 to 19.3%. the two anionic salmon-trypsin complexes (st-cpti and st-cmti) and the bovine-trypsin complex (bt-cpti) have been ...199910089404
elicitin 172 from an isolate of phytophthora nicotianae pathogenic to tomato.elicitin 172, an acid protein with elicitor activity, has been isolated in true form from culture filtrates of phytophthora nicotianae, the causal agent of crown and root rot of tomato (lycopersicon esculentum). the m(r) (10,349 +/- 1) of the purified protein, determined by es-ms, is identical to that calculated for parasiticein using the mean isotopic composition and assuming the occurrence of three disulfide bridges. the primary structure of elicitin 172, determined using also maldi-ms experim ...199910192962
molecular cloning and tissue-specific expression of an anionic peroxidase in zucchini.a calcium-pectate-binding anionic isoperoxidase (aprx) from zucchini (cucurbita pepo) was purified and subjected to n-terminal amino acid microsequencing. the cdna encoding this enzyme was obtained by reverse transcriptase polymerase chain reaction from a cdna library. it encoded a mature protein of 309 amino acids exhibiting all of the sequence characteristics of a plant peroxidase. despite the presence of a c-terminal propeptide, aprx was found in the apoplast. aprx protein and mrna were found ...199910398715
comparative study of the chemical composition and nutritional value of pumpkin seed cake, soybean meal and casein.the chemical composition and nutritional value of pumpkin (cucurbita pepo) seed cake (psc) were studied and compared with those of casein and soybean meal. crude protein and ether extract content in dry matter of psc was 598.0 g/kg and 124.6 g/kg, respectively, and was higher than the percentage of these components (474.2 and 28.3 g/kg, respectively) in soybean meal used in this experiment. the main fatty acids in psc cake were oleic acid (50.4%) and linoleic acid (29.9%). protein found in psc c ...199910633538
rna aptamers that bind to and inhibit the ribosome-inactivating protein, pepocin.pepocin, isolated from cucurbita pepo, is a ribosome-inactivating protein (rip). rips site-specifically recognize and depurinate an adenosine at position 4324 in rat 28 s rrna, rendering the ribosome incapable of interacting with essential elongation factors. aptamers that target pepocin were isolated from a degenerate rna pool by in vitro selection. a conserved hairpin motif, quite different from the sequence of the toxin-substrate domain in rat 28 s rrna, was identified in the aptamer sequence ...200010671532
purification and characterization of a novel pumpkin short-chain acyl-coenzyme a oxidase with structural similarity to acyl-coenzyme a dehydrogenases.a novel pumpkin (cucurbita pepo) short-chain acyl-coenzyme a (coa) oxidase (acox) was purified to homogeneity by hydrophobic-interaction, hydroxyapatite, affinity, and anion-exchange chromatography. the purified enzyme is a tetrameric protein, consisting of apparently identical 47-kd subunits. the protein structure of this oxidase differs from other plant and mammalian acoxs, but is similar to the protein structure of mammalian mitochondrial acyl-coa dehydrogenase (acdh) and the recently identif ...200010806249
african cucurbita pepo l.: properties of seed and variability in fatty acid composition of seed oil.pumpkin (cucurbita pepo) seeds are used locally in eritrea to treat tapeworm. seeds were found to be rich in oil (approximately 35%), protein (38%), alpha-tocoferols (3 mg/100 g) and carbohydrate content (approximately 37%). the physico-chemical properties and fatty acid composition of the seed oil were examined. the four dominant fatty acids found are: palmitic c16:0 (13.3%), stearic c18:0 (8.0%), oleic c18:1 (29.0%) and linoleic c18:2 (47.0%). the oil contains an appreciable amount of unsatura ...200010846750
effect of silver reflective mulch and a summer squash trap crop on densities of immature bemisia argentifolii (homoptera: aleyrodidae) on organic bean.polyethylene mulch with a reflective silver stripe and a yellow summer squash, cucurbita pepo l., trap crop were tested alone and in combination as tactics to reduce densities of bemisia argentifolii bellows & perring eggs and nymphs, and incidence of bean golden mosaic geminivirus on snap bean, phaseolus vulgaris l. egg densities were consistently higher on squash than on bean, but egg densities and virus incidence were not lower on bean grown with squash than on bean grown in monoculture. silv ...200010902322
quiescent intense (qi): a gene that affects young but not mature fruit color intensity in cucurbita pepo.jack o'lantern, a cultivar of the pumpkin group of cucurbita pepo that has intense-colored young fruits, was crossed with vegetable spaghetti, a cultivar of the vegetable marrow group that has light-colored young fruits and genotype d/d i-1/i-1 i-2/i-2. the f1 plants, regardless of the direction of the cross, had light-colored young fruits. young fruit color was observed to segregate in accordance with a 3 intense: 13 light ratio in the f2, indicating that two genes, one dominant and one recessi ...200010912682
allergy caused by ingestion of zucchini (cucurbita pepo): characterization of allergens and cross-reactivity to pollen and other foods.allergy to zucchini (cucurbita pepo), a member of the cucurbitaceae family, has not previously been reported. we examined 4 patients complaining of allergic symptoms, such as oral allergy syndrome, nausea, diarrhea, or pruritus, after the intake of zucchini.200010932084
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