gossypium barbadense genome sequence provides insight into the evolution of extra-long staple fiber and specialized metabolites.of the two cultivated species of allopolyploid cotton, gossypium barbadense produces extra-long fibers for the production of superior textiles. we sequenced its genome (ad)2 and performed a comparative analysis. we identified three bursts of retrotransposons from 20 million years ago (mya) and a genome-wide uneven pseudogenization peak at 11-20 mya, which likely contributed to genomic divergences. among the 2,483 genes preferentially expressed in fiber, a cell elongation regulator, pre1, is stri ...201526420475
molecular cloning and functional analysis of gbrvd, a gene in gossypium barbadense that plays an important role in conferring resistance to verticillium wilt.most of the disease resistance genes already characterized in plants encode nucleotide-binding site-leucine rich repeat (nbs-lrr) proteins that have key roles in resistance to verticillium dahliae. using a cdna library and race protocols, we cloned a coiled-coil (cc)-nbs-lrr-type gene, gbrvd, from a resistant tetraploid cotton species, gossypium barbadense (rvd=resistance to v. dahliae). we also applied rt-qpcr and vigs technologies to analyze how expression of gbrvd was induced upon attack by v ...201626407869
steam explosion distinctively enhances biomass enzymatic saccharification of cotton stalks by largely reducing cellulose polymerization degree in g. barbadense and g. this study, steam explosion pretreatment was performed in cotton stalks, leading to 5-6 folds enhancements on biomass enzymatic saccharification distinctive in gossypium barbadense and gossypium hirsutum species. sequential 1% h2so4 pretreatment could further increase biomass digestibility of the steam-exploded stalks, and also cause the highest sugar-ethanol conversion rates probably by releasing less inhibitor to yeast fermentation. by comparison, extremely high concentration alkali (16% na ...201525656866
genetic effects and heterosis of yield and yield component traits based on gossypium barbadense chromosome segment substitution lines in two gossypium hirsutum backgrounds.we hybridized 10 chromosome segment substitution lines (cssls) each from two cssl populations and produced 50 f1 hybrids according to north carolina design ii. we analyzed the genetic effects and heterosis of yield and yield components in the f1 hybrids and parents in four environments via the additive-dominance genetic model. yield and yield components of the cssls were controlled by combined additive and dominance effects, and lint percentage was mainly controlled by additive effects, but boll ...201627348815
metabolomic and transcriptomic insights into how cotton fiber transitions to secondary wall synthesis, represses lignification, and prolongs elongation.the morphogenesis of single-celled cotton fiber includes extreme elongation and staged cell wall differentiation. designing strategies for improving cotton fiber for textiles and other uses relies on uncovering the related regulatory mechanisms. in this research we compared the transcriptomes and metabolomes of two gossypium genotypes, gossypium barbadense cv phytogen 800 and g. hirsutum cv deltapine 90. when grown in parallel, the two types of fiber developed similarly except for prolonged fibe ...201526116072
diel trends in stomatal response to ozone and water deficit: a unique relationship of midday values to growth and allometry in pima cotton?plant responses to ozone (o3 ) and water deficit (wd) are commonly observed, although less is known about their interaction. stomatal conductance (gs ) is both an impact of these stressors and a protective response to them. stomatal closure reduces inward flux of o3 and outward flux of water. stomatal measurements are generally obtained at midday when gas exchange is maximal, but these may not be adequate surrogates for stomatal responses observed at other times of day, nor for non-stomatal resp ...201626031549
molecular cloning and analysis of a receptor-like promoter of gbvdr3 gene in sea island cotton.verticillium wilt caused by soil borne fungus verticillium dahliae could significantly reduce cotton yield. the ve1 homologous gene gbvdr3 is resistant to verticillium wilt. in order to understand of the function of the promoter gbvdr3 in gossypium barbadense, the promoter region of the receptor-like gene gbvdr3 was obtained by genome walking, and the cis-element in the promoter was identified using the place software in this study. the sequence analysis showed that the promoter contained elemen ...201627323087
independent domestication of two old world cotton species.domesticated cotton species provide raw material for the majority of the world's textile industry. two independent domestication events have been identified in allopolyploid cotton, one in upland cotton (gossypium hirsutum l.) and the other to egyptian cotton (gossypium barbadense l.). however, two diploid cotton species, gossypium arboreum l. and gossypium herbaceum l., have been cultivated for several millennia, but their status as independent domesticates has long been in question. using geno ...201627289095
a comparative transcriptome analysis of two sets of backcross inbred lines differing in lint-yield derived from a gossypium hirsutum × gossypium barbadense population.upland cotton (gossypium hirsutum l.) is the most important fiber crop, and its lint-yield improvement is impeded due to its narrow genetic base and the lack of understanding of the genetic basis of yield. backcross inbred lines (bils) or near-isogenic lines (nils) in the same genetic background differing in lint yield, developed through advanced backcrossing, provide an important genomic resource to study the molecular genetic basis of lint yield. in the present study, a high-yield (hy) group a ...201627256327
to be a flower or fruiting branch: insights revealed by mrna and small rna transcriptomes from different cotton developmental stages.the architecture of the cotton plant, including fruit branch formation and flowering pattern, is the most important characteristic that directly influences light exploitation, yield and cost of planting. nulliplex branch is a useful phenotype to study cotton architecture. we used rna sequencing to obtain mrna and mirna profiles from nulliplex- and normal-branch cotton at three developmental stages. the differentially expressed genes (degs) and mirnas were identified that preferentially/specifica ...201626983497
the thioredoxin gbnrx1 plays a crucial role in homeostasis of apoplastic reactive oxygen species in response to verticillium dahliae infection in cotton.examining the proteins that plants secrete into the apoplast in response to pathogen attack provides crucial information for understanding the molecular mechanisms underlying plant innate immunity. in this study, we analyzed the changes in the root apoplast secretome of the verticillium wilt-resistant island cotton cv hai 7124 (gossypium barbadense) upon infection with verticillium dahliae two-dimensional differential gel electrophoresis and matrix-assisted laser desorption/ionization tandem tim ...201626869704
deep-tissue confocal imaging of the central projections of ovipositor sensory afferents in the egyptian cotton leafworm, spodoptera littoralis.the pre-ovipositon behavior of moths is largely dependent upon the cues that a gravid female perceives while assessing potential oviposition sites. assessment of such sites is accomplished, at least in part, by mechanosensory and gustatory sensilla located on the ovipositor whose sensory neurons project into the terminal abdominal ganglion (tag). using anterograde backfill staining, confocal laser scanning microscopy, and three dimensional reconstruction, we traced and analyzed the central proje ...201626774745
genetic diversity of the two commercial tetraploid cotton species in the gossypium diversity reference set.a diversity reference set has been constructed for the gossypium accessions in the us national cotton germplasm collection to facilitate more extensive evaluation and utilization of accessions held in the collection. a set of 105 mapped simple sequence repeat markers was used to study the allelic diversity of 1933 tetraploid gossypium accessions representative of the range of diversity of the improved and wild accessions of g. hirsutum and g. barbadense. the reference set contained 410 g. barbad ...201626774060
breeding potential of introgression lines developed from interspecific crossing between upland cotton (gossypium hirsutum) and gossypium barbadense: heterosis, combining ability and genetic effects.upland cotton (gossypium hirstum l.), which produces more than 95% of the world natural cotton fibers, has a narrow genetic base which hinders progress in cotton breeding. introducing germplasm from exotic sources especially from another cultivated tetraploid g. barbadense l. can broaden the genetic base of upland cotton. however, the breeding potential of introgression lines (ils) in upland cotton with g. barbadense germplasm integration has not been well addressed. this study involved six ils ...201626730964
cotton fiber elongation network revealed by expression profiling of longer fiber lines introgressed with different gossypium barbadense chromosome segments.cotton fiber, a highly elongated, thickened single cell of the seed epidermis, is a powerful cell wall research model. fiber length, largely determined during the elongation stage, is a key property of fiber quality. several studies using expressed sequence tags and microarray analysis have identified transcripts that accumulate preferentially during fiber elongation. to further show the mechanism of fiber elongation, we used digital gene expression tag profiling to compare transcriptome data fr ...201425273845
constructing a high-density linkage map for gossypium hirsutum × gossypium barbadense and identifying qtls for lint introgress the good fiber quality and yield from gossypium barbadense into a commercial upland cotton variety, a high-density simple sequence repeat (ssr) genetic linkage map was developed from a bc1 f1 population of gossypium hirsutum × gossypium barbadense. the map comprised 2,292 loci and covered 5115.16 centimorgan (cm) of the cotton ad genome, with an average marker interval of 2.23 cm. of the marker order for 1,577 common loci on this new map, 90.36% agrees well with the marker order on ...201525263268
characterization of the global transcriptome for cotton (gossypium hirsutum l.) anther and development of ssr marker.cotton is an important fiber plant, and it's attractive to elucidate the molecular mechanism of anther development due to the close relationship between the anther fertility and boll-setting, and also fiber yield. in the present paper, 47.2 million paired-end reads with average length of 82.87 bp from the anthers of tm-1 (gossypium hirsutum l.), a genetic standard line, were generated through transcriptome sequencing, and 210,965 unigenes of more than 100 bp were obtained. blast, kegg, cog, and ...201425178523
a ve homologous gene from gossypium barbadense, gbvdr3, enhances the defense response against verticillium dahliae.the tomato ve1 gene and several ve1 homologues are involved in the resistance to verticillium dahliae. here, we report on another ve homologous gene, gbvdr3, from a verticillium wilt-resistant cotton cultivar, gossypium barbadense hai7124, which has a 3207-bp region that encodes a predicted receptor-like protein. transient expression analyses indicated that gbvdr3 is localized in the plasma membrane, and virus-induced gene silencing of gbvdr3 compromised the resistance of hai7124 cotton to a def ...201626686282
the genome sequence of sea-island cotton (gossypium barbadense) provides insights into the allopolyploidization and development of superior spinnable fibres.gossypium hirsutum contributes the most production of cotton fibre, but g. barbadense is valued for its better comprehensive resistance and superior fibre properties. however, the allotetraploid genome of g. barbadense has not been comprehensively analysed. here we present a high-quality assembly of the 2.57 gigabase genome of g. barbadense, including 80,876 protein-coding genes. the double-sized genome of the a (or at) (1.50 gb) against d (or dt) (853 mb) primarily resulted from the expansion o ...201526634818
behavioral and metabolic effects of sublethal doses of two insecticides, chlorpyrifos and methomyl, in the egyptian cotton leafworm, spodoptera littoralis (boisduval) (lepidoptera: noctuidae).insecticides have long been used as the main method in limiting agricultural pests, but their widespread use has resulted in environmental pollution, development of resistances, and biodiversity reduction. the effects of insecticides at low residual doses on both the targeted crop pest species and beneficial insects have become a major concern. in particular, these low doses can induce unexpected positive (hormetic) effects on pest insects, such as surges in population growth exceeding what woul ...201626566611
the amino acid sequence of cytochrome c from abutilon theophrasti medic. and gossypium barbadense l. (cotton).the amino acid sequences of abutilon and gossypium cytochromes c were determined on 1mumol of protein. the molecules consist of 111 residues and are homologous with other mitochondrial plant cytochromes c. experimental details are given in a supplementary paper that has been deposited as supplementary publication sup 50005 at the national lending library for science and technology, boston spa, yorks. ls23 7bq, u.k., from whom copies can be obtained on the terms indicated in biochem. j. (1971), 1 ...19715131735
rapid evolutionary divergence of gossypium barbadense and g. hirsutum mitochondrial genomes.the mitochondrial genome from upland cotton, g. hirsutum, was previously sequenced. to elucidate the evolution of mitochondrial genomic diversity within a single genus, we sequenced the mitochondrial genome from sea island cotton (gossypium barbadense l.).201526459858
a cotton fiber-preferential promoter, pgbexpa2, is regulated by ga and aba in arabidopsis.pgbexpa2 (promoter of gbexpa2 ) was preferentially and strongly expressed during cotton fiber development, and the 461-bp pgbexpa2 fragment was essential for responding to exogenous ga and aba in arabidopsis. cotton fibers are highly elongated single-cell, unbranched and non-glandular seed trichomes. previous studies have reported that the transcript level of gbexpa2 is significantly up-regulated during fiber cell elongation, suggesting that gbexpa2 has an important function in fiber development ...201526001998
inhibition kinetics of acid and alkaline phosphatases by atrazine and methomyl pesticides.the main objective of this work was to investigate the kinetic characteristics of acid and alkaline phosphatases isolated from different sources and to study the effects of the herbicide atrazine and insecticide methomyl on the activity and kinetic properties of the enzymes. acid phosphatase (acp) was isolated from the tomato plant (solanum lycopersicum l. var. lycopersicum); alkaline phosphatase (alp) was isolated from two sources, including mature earthworms (aporrectodea caliginosa) and larva ...201525996812
molecular characterization of a transcriptionally active ty1/copia-like retrotransposon in gossypium.a transcriptionally active ty1/copia -like retrotransposon was identified in the genome of gossypium barbadense. the different heat activation of this element was observed in two tetraploid cotton species. most retrotransposons from plants are transcriptionally silent, or activated under certain conditions. only a small portion of elements are transcriptionally active under regular condition. a long terminal repeat (ltr) retrotransposon was isolated from the cultivated sea island cotton (h7124) ...201525693493
functional analysis of the seed coat-specific gene gbmyb2 from cotton.myb transcription factors are essential for cotton fiber development. we isolated the r2r3-myb gene gbmyb2 from gossypium barbadense. rna in situ hybridization analysis showed that gbmyb2 is mainly expressed in the outer integuments of cotton ovules and in elongating fibers. gbmyb2 expression increased throughout fiber initiation and during the elongation stage. the expression level of gbmyb2 was higher in the gossypium hirsutum cultivar xu142 than in the xu142 (fl) mutant. overexpression of gbm ...201324036393
distribution of neuropeptides in the antennal lobes of male spodoptera littoralis.olfaction is an important sensory modality that regulates a plethora of behavioural expressions in insects. processing of olfactory information takes place in the primary olfactory centres of the brain, namely the antennal lobes (als). neuropeptides have been shown to be present in the olfactory system of various insect species. in the present study, we analyse the distribution of tachykinin, fmrfamide-related peptides, allatotropin, allatostatin, myoinhibitory peptides and sifamide in the al of ...201323955643
bioinformatics analysis of small rnas in pima (gossypium barbadense l.).small rnas (srnas) are ~20 to 24 nucleotide single-stranded rnas that play crucial roles in regulation of gene expression. in plants, srnas are classified into micrornas (mirnas), repeat-associated sirnas (ra-sirnas), phased sirnas (pha-sirnas), cis and trans natural antisense transcript sirnas (cis- and trans-nat sirnas). pima (gossypium barbadense l.) is one of the most economically important fiber crops, producing the best and longest spinnable fiber. although some mirnas are profiled in pima ...201525679373
specific response to herbivore-induced de novo synthesized plant volatiles provides reliable information for host plant selection in a moth.animals depend on reliable sensory information for accurate behavioural decisions. for herbivorous insects it is crucial to find host plants for feeding and reproduction, and these insects must be able to differentiate suitable from unsuitable plants. volatiles are important cues for insect herbivores to assess host plant quality. it has previously been shown that female moths of the egyptian cotton leafworm, spodoptera littoralis (lepidoptera: noctuidae), avoid oviposition on damaged cotton gos ...201323737555
why urban citizens in developing countries use traditional medicines: the case of suriname.the use of traditional medicines (tms) among urban populations in developing countries and factors underlying people's decision to use tms are poorly documented. we interviewed 270 adults in paramaribo, suriname, using a stratified random household sample, semistructured questionnaires, and multivariate analysis. respondents mentioned 144 medicinal plant species, most frequently gossypium barbadense, phyllanthus amarus, and quassia amara. 66% had used tms in the previous year, especially people ...201323653663
truncated cotton subtilase promoter directs guard cell-specific expression of foreign genes in tobacco and arabidopsis.a 993-bp regulatory region upstream of the translation start codon of subtilisin-like serine protease gene was isolated from gossypium barbadense. this (t/a)aaag-rich region, gbslsp, and its 5'- and 3'-truncated versions were transferred into tobacco and arabidopsis after fusing with gus or gfp. histochemical and quantitative gus analysis and confocal gfp fluorescence scanning in the transgenic plants showed that the gbslsp-driven gus and gfp expressed preferentially in guard cells, whereas driv ...201323555786
a cotton myb transcription factor, gbmyb5, is positively involved in plant adaptive response to drought stress.drought stress negatively affects plant growth and limits plant productivity. genes functioning in plant responses to drought stress are essential for the development of drought-tolerant crops. here, we report that an r2r3-type myb transcription factor gene in gossypium barbadense, gbmyb5, confers drought tolerance in cotton and transgenic tobacco. virus-induced gene silencing of gbmyb5 compromised the tolerance of cotton plantlets to drought stress and reduced the post-rewatering water recovery ...201525657343
assessing the total factor productivity of cotton production in egypt.the main objective of this paper is to decompose the productivity growth of egyptian cotton production. we employ the stochastic frontier approach and decompose the changes in total factor productivity (ctfp) growth into four components: technical progress (tp), changes in scale component (csc), changes in allocative efficiency (cae), and changes in technical efficiency (cte). considering a situation of scarce statistical information, we propose four alternative empirical models, with the purpos ...201525625318
characterization of expressed sequence tags from developing fibers of gossypium barbadense and evaluation of insertion-deletion variation in tetraploid cultivated cotton species.cotton is the leading fiber crop worldwide. gossypium barbadense is an important species of cotton because of its extra-long staple fibers with superior luster and silkiness. however, a systematic analysis and utilization of cdna sequences from g. barbadense fiber development remains understudied.201323497047
identification and characterization of a fatty acyl reductase from a spodoptera littoralis female gland involved in pheromone biosynthesis.fatty acyl-coa reductases (fars), the enzymes that catalyse reduction of a fatty acyl-coa to the corresponding alcohol in insect pheromone biosynthesis, are postulated to play an important role in determining the proportion of each component in the pheromone blend. for the first time, we have isolated and characterized from the egyptian cotton leaf worm spodoptera littoralis (lepidoptera: noctuidae) a far cdna (slit-far1), which appeared to be expressed only in the pheromone gland and was undete ...201525558806
longitudinal assessment of occupational exposures to the organophosphorous insecticides chlorpyrifos and profenofos in egyptian cotton field workers.chlorpyrifos (cpf) and profenofos (pff) are organophosphorus (op) insecticides that are applied seasonally in egypt to cotton fields. urinary trichloro-2-pyridinol (tcpy), a specific cpf metabolite, and 4-bromo-2-chlorophenol (bcp), a specific pff metabolite, are biomarkers of exposure, while inhibition of blood butyrylcholinesterase (bche) and acetylcholinesterase (ache) activities are effect biomarkers that may be associated with neurotoxicity. urinary tcpy and bcp and blood bche and ache acti ...201525466362
demonstration of a diel trend in sensitivity of gossypium to ozone: a step toward relating o₃ injury to exposure or flux.plant injury by ozone (o3) occurs in three stages, o3 entrance through stomata, overcoming defences, and attack on bioreceptors. concentration, deposition, and uptake of o3 are accessible by observation and modelling, while injury can be assessed visually or through remote sensing. however, the relationship between o3 metrics and injury is confounded by variation in sensitivity to o3. sensitivity weighting parameters have previously been assigned to different plant functional types and growth st ...201323404900
construction of genetic linkage map with chromosomal assigment and quantitative trait loci associated with some important agronomic traits in cotton.cotton is the world's leading natural fiber and second most important oilseed crop and has been a focus of genetic, systematic and breeding research. the genetic and physiological bases of some important agronomic traits in cotton were investigated by qtl mapping through constructing of genetic map with chromosomal assignment. a segregating f2 population derived from an interspecific cross (g. barbadense x g. hirsutum) between two genotypes, cvs. "giza 83" and "deltapine" was used in this study. ...201423333856
direct visualization of horizontal gene transfer in cotton plants.plant mitochondrial and chloroplast genes that underwent horizontal transfer have been identified by parasite and grafting systems, respectively. here, we directly observed 3 horizontal gene transfer (hgt) events in the 45 second axillary shoots of grafted cotton plants (gossypium barbadense and gossypium hirsutum) after extirpating the first axillary bud. the second axillary shoots showed phenotypic variations in cotton flowers and seeds that were evidence of spontaneous development from cells ...201425160847
small rna and degradome profiling reveals a role for mirnas and their targets in the developing fibers of gossypium barbadense.micrornas (mirnas) are 20-24 nucleotide non-coding small rnas that play important roles in plant development. the stages of cotton fiber development include initiation, elongation, secondary wall thickening (swt) and maturation. we constructed seven fiber rna libraries representing the initiation, elongation and swt stages. in total, 47 conserved mirna families and seven candidate mirnas were profiled using small rna sequencing. northern blotting and real-time polymerase chain reaction (pcr) ana ...201425131375
quantitative and ultrastructural changes in the haemocytes of spodoptera littoralis (boisd.) treated individually or in combination with spodoptera littoralis multicapsid nucleopolyhedrovirus (splimnpv) and azadirachtin.the total haemocyte count (thc) and the possible ultrastructural alterations induced in the haemocytes of the fourth larval instars of the egyptian cotton leafworm, spodoptera littoralis (boisd.) (lepidoptera: noctuidae), 96 h post-feeding on a semi-synthetic diet, treated with the lc50 of spodoptera littoralis multicapsid nucleopolyhedrovirus (splimnpv) and the lc50 of azadirachtin alone, and the lc25 of splimnpv combined with the lc25 of azadirachtin were studied and compared to the control. s ...201425041832
transcriptomic analysis of fiber strength in upland cotton chromosome introgression lines carrying different gossypium barbadense chromosomal segments.fiber strength is the key trait that determines fiber quality in cotton, and it is closely related to secondary cell wall synthesis. to understand the mechanism underlying fiber strength, we compared fiber transcriptomes from different g. barbadense chromosome introgression lines (csils) that had higher fiber strengths than their recipient, g. hirsutum acc. tm-1. a total of 18,288 differentially expressed genes (degs) were detected between csil-35431 and csil-31010, two csils with stronger fiber ...201424762562
signaling pathways controlling induced resistance to insect herbivores in arabidopsis.insect attack triggers changes in transcript level in plants that are mediated predominantly by jasmonic acid (ja). the implication of ethylene (et), salicylic acid (sa), and other signals in this response is less understood and was monitored with a microarray containing insect- and defense-regulated genes. arabidopsis thaliana mutants coi1-1, ein2-1, and sid2-1 impaired in ja, et, and sa signaling pathways were challenged with the specialist small cabbage white (pieris rapae) and the generalist ...200717977152
overexpression of gbwrky1 positively regulates the pi starvation response by alteration of auxin sensitivity in arabidopsis.overexpression of a cotton defense-related gene gbwrky1 in arabidopsis resulted in modification of the root system by enhanced auxin sensitivity to positively regulate the pi starvation response. gbwrky1 was a cloned wrky transcription factor from gossypium barbadense, which was firstly identified as a defense-related gene and showed moderate similarity with atwrky75 from arabidopsis thaliana. overexpression of gbwrky1 in arabidopsis resulted in attenuated pi starvation stress symptoms, includin ...201222890372
gbexpatr, a species-specific expansin, enhances cotton fibre elongation through cell wall restructuring.cotton provides us the most important natural fibre. high fibre quality is the major goal of cotton breeding, and introducing genes conferring longer, finer and stronger fibre from gossypium barbadense to gossypium hirsutum is an important breeding strategy. we previously analysed the g. barbadense fibre development mechanism by gene expression profiling and found two homoeologous fibre-specific α-expansins from g. barbadense, gbexpa2 and gbexpatr. gbexpa2 (from the dt genome) is a classical α-e ...201626269378
molecular cloning and characterization of enhanced disease susceptibility 1 (eds1) from gossypium barbadense.arabidopsis enhanced disease susceptibility 1 (eds1) plays an important role in plant defense against biotrophic and necrotrophic pathogens. the necrotrophic pathogen verticillium dahliae infection of gossypium barbadense could lead to verticillium wilt which seriously reduces the cotton production. here, we cloned and characterized a g. barbadense homolog of eds1, designated as gbeds1. the full-length cdna of the gbeds1 gene was obtained by the technique of rapid-amplification of cdna ends. the ...201424570021
the hairless stem phenotype of cotton (gossypium barbadense) is linked to a copia-like retrotransposon insertion in a homeodomain-leucine zipper gene (hd1).cotton (gossypium) stem trichomes are mostly single cells that arise from stem epidermal cells. in this study, a homeodomain-leucine zipper gene (hd1) was found to cosegregate with the dominant trichome locus previously designated as t1 and mapped to chromosome 6. characterization of hd1 orthologs revealed that the absence of stem trichomes in modern gossypium barbadense varieties is linked to a large retrotransposon insertion in the ninth exon, 2565 bp downstream from the initial codon in the a ...201526133897
association analysis of yield and fiber quality traits in gossypium barbadense with ssrs and sraps.cotton is an important cash crop. mining for quantitative trait loci related to yield and fiber quality traits using association analysis has many advantages for cotton research. in this study, 170 simple sequence repeats (ssrs) and 258 sequence-related amplified polymorphisms (sraps) were used to analyze the association of 3 yield component traits and 5 fiber quality traits of 55 gossypium barbadense accessions in 2009 and 2010. principal component analysis of ssrs and sraps showed 3 and 2 subg ...201324065676
transcriptome profiling of gossypium barbadense inoculated with verticillium dahliae provides a resource for cotton improvement.verticillium wilt, caused by the fungal pathogen verticillium dahliae, is the most severe disease in cotton (gossypium spp.), causing great lint losses worldwide. disease management could be achieved in the field if genetically improved, resistant plants were used. however, the interaction between v. dahliae and cotton is a complicated process, and its molecular mechanism remains obscure. to understand better the defense response to this pathogen as a means for obtaining more tolerant cultivars, ...201324053558
genetic diversity of sea-island cotton (gossypium barbadense) revealed by mapped order to evaluate the genetic diversity of sea-island cotton (gossypium barbadense), 237 commonly mapped ssr markers covering the cotton genome were used to genotype 56 sea-island cotton accessions. a total of 218 polymorphic primer pairs (91.98%) amplified 361 loci, with a mean of 1.66 loci. polymorphism information content values of the ssr primers ranged from 0.035 to 0.862, with a mean of 0.320. the highest mean polymorphism information content value for the ssr motifs was from a compound ...201122183945
peripheral regulation by ecdysteroids of olfactory responsiveness in male egyptian cotton leaf worms, spodoptera littoralis.physiological and behavioral plasticity allows animals to adapt to changes in external (environmental) and internal (physiological) factors. in insects, the physiological state modulates adult behavior in response to different odorant stimuli. hormones have the potential to play a major role in the plasticity of the olfactory responses. to explore if peripheral olfactory processing could be regulated by steroid hormones, we characterized the molecular, electrophysiological, and behavioral respon ...201222044719
proteomic profiling of developing cotton fibers from wild and domesticated gossypium barbadense.pima cotton (gossypium barbadense) is widely cultivated because of its long, strong seed trichomes ('fibers') used for premium textiles. these agronomically advanced fibers were derived following domestication and thousands of years of human-mediated crop improvement. to gain an insight into fiber development and evolution, we conducted comparative proteomic and transcriptomic profiling of developing fiber from an elite cultivar and a wild accession. analyses using isobaric tag for relative and ...201323795774
a genetic and metabolic analysis revealed that cotton fiber cell development was retarded by flavonoid naringenin.the cotton (gossypium spp.) fiber is a unique elongated cell that is useful for investigating cell differentiation. previous studies have demonstrated the importance of factors such as sugar metabolism, the cytoskeleton, and hormones, which are commonly known to be involved in plant cell development, while the secondary metabolites have been less regarded. by mining public data and comparing analyses of fiber from two cotton species (gossypium hirsutum and gossypium barbadense), we found that th ...201323535943
an integrative analysis of four cesa isoforms specific for fiber cellulose production between gossypium hirsutum and gossypium barbadense.cotton fiber is an excellent model system of cellulose biosynthesis; however, it has not been widely studied due to the lack of information about the cellulose synthase (cesa) family of genes in cotton. in this study, we initially identified six full-length cesa genes designated as ghcesa5-ghcesa10. phylogenetic analysis and gene co-expression profiling revealed that cesa1, cesa2, cesa7, and cesa8 were the major isoforms for secondary cell wall biosynthesis, whereas cesa3, cesa5, cesa6, cesa9, a ...201323508664
genetical and biological control of cotton ashy stem caused by macrophomina phaseolina in outdoor pot experiment.two outdoor pot experiments were carried out to evaluate the reaction of 11 commercial egyptian cotton cultivars macrophomina phaseolina, the incitant of ashy stem in cotton and to evaluate the antagonistic ability of 27 isolates of trichoderma sp. against pathogen cotton cultivars giza 85, giza 87, giza 89 and giza 90 were resistant to m. phaseolina because both survival and plant height of these cultivars was not affected when the soil was infested with the pathogen. none of the cultivars were ...201023961071
cotton defense induction patterns under spatially, temporally and quantitatively varying herbivory its defense against herbivores, cotton (gossypium sp.) relies in part on the production of a set of inducible, non-volatile terpenoids. under uniform damage levels, in planta allocation of induced cotton terpenoids has been found to be highest in youngest leaves, supporting assumptions of the optimal defense theory (odt) which predicts that plants allocate defense compounds to tissues depending on their value and the likelihood of herbivore attack. however, our knowledge is limited on how var ...201728270830
local and differential control of vegetative storage protein expression in response to herbivore damage in arabidopsis thaliana.vegetative storage proteins (vsps) are thought to fulfil important nutritional roles during plant development and stress adaptation. plant responses to mechanical wounding and herbivore damage include an activation of vsp expression. it was recently suggested that vsp is part of the systemic response of arabidopsis to wounding. to test this proposal, we monitored the spatial regulation of vsp mrnas and vsp proteins. arabidopsis contains two vsp genes and real-time quantitative pcr allowed us to ...200211982938
a receptor-like kinase gene (gbrlk) from gossypium barbadense enhances salinity and drought-stress tolerance in arabidopsis.cotton (gossypium spp.) is widely cultivated due to the important economic value of its fiber. however, extreme environmental degradation impedes cotton growth and production. receptor-like kinase (rlk) proteins play important roles in signal transduction and participate in a diverse range of processes in response to plant hormones and environmental cues. here, we introduced an rlk gene (gbrlk) from cotton into arabidopsis and investigated its role in imparting abiotic stress tolerance.201323915077
antisense expression of gossypium barbadense ugd6 in arabidopsis thaliana significantly alters cell wall composition.uridine diphosphate-glucose dehydrogenase (ugd, ec1.1.1.22 oxidizes udp-glc (udp-d-glucose) to udp-glca (udp-d-glucuronate), a critical precursor of cell wall polysaccharides. gbugd6 from gossypium barbadense is more highly expressed late in the elongation of cotton fibers (15 d post-anthesis (dpa)) and during the stage of secondary cell wall thickening (30 dpa). subcellular localization analysis in onion epidermis revealed that fluorescently labeled gbugd6 protein was distributed throughout the ...201626810898
island cotton enhanced disease susceptibility 1 gene encoding a lipase-like protein plays a crucial role in response to verticillium dahliae by regulating the sa level and h2o2 accumulation.cotton is one of the most economically important crops, but most cultivated varieties lack adequate innate immunity or resistance to verticillium wilt. this results in serious losses to both yield and fiber quality. to identify the genetic resources for innate immunity and understand the pathways for pathogen defenses in this crop, here we focus on orthologs of the central arabidopsis thaliana defense regulator enhanced disease susceptibility 1 (eds1). the full-length cdna of gbeds1 was obtained ...201628018374
modulation of reproductive behaviors by non-host volatiles in the polyphagous egyptian cotton leafworm, spodoptera order to locate mates, food, and oviposition sites, insects mainly rely on volatile cues released by their sexual partners, food sources, and host and non-host plants. calling, mating, and oviposition behaviors, as well as fecundity and longevity, of newly emerged spodoptera littoralis (bois.) moths were recorded in the presence of volatiles from leaves of a host plant, gossypium hirsutum (cotton) and two non-host plants, adhatoda vasica (av) or picea abies (spruce), either alone or in host/n ...201324105603
a cotton annexin protein anxgb6 regulates fiber elongation through its interaction with actin 1.annexins are assumed to be involved in regulating cotton fiber elongation, but direct evidence remains to be presented. here we cloned six annexin genes (anxgb) abundantly expressed in fiber from sea-island cotton (g. barbadense). qrt-pcr results indicated that all six g. barbadense annexin genes were expressed in elongating cotton fibers, while only the expression of anxgb6 was cotton fiber-specific. yeast two hybridization and bifc analysis revealed that anxgb6 homodimer interacted with a cott ...201323750279
inheritance of long staple fiber quality traits of gossypium barbadense in g. hirsutum background using csils.gossypium hirsutum is a high yield cotton species that exhibits only moderate performance in fiber qualities. a promising but challenging approach to improving its phenotypes is interspecific introgression, the transfer of valuable traits or genes from the germplasm of another species such as g. barbadense, an important cultivated extra long staple cotton species. one set of chromosome segment introgression lines (csils) was developed, where tm-1, the genetic standard in g. hirsutum, was used as ...201222297564
an l1 box binding protein, gbml1, interacts with gbmyb25 to control cotton fibre development.transcription factors play key roles in plant development through their interaction with cis-elements and/or other transcription factors. a hd-zip iv family transcription factor, gossypium barbadense meristem layer 1 (gbml1) has been identified and characterized here. gbml1 specifically bound to the l1 box and the promoters of gbml1 and gbrdl1. gbml1 physically interacted with a key regulator of cotton fibre development, gbmyb25. truncated and point mutation assays indicated the start-sad domain ...201020667961
cotton fiber cell walls of gossypium hirsutum and gossypium barbadense have differences related to loosely-bound xyloglucan.cotton fiber is an important natural textile fiber due to its exceptional length and thickness. these properties arise largely through primary and secondary cell wall synthesis. the cotton fiber of commerce is a cellulosic secondary wall surrounded by a thin cuticulated primary wall, but there were only sparse details available about the polysaccharides in the fiber cell wall of any cotton species. in addition, gossypium hirsutum (gh) fiber was known to have an adhesive cotton fiber middle lamel ...201323457548
desoxyhemigossypol 6-methyl ether.the title sesquiterpene [systematic name: 6-methoxy-10-methyl-7-(propan-2-yl)-2-oxatricyclo[,12)]dodeca-1(11),4,6,8(12),9-pentaen-5-ol], c(16)h(18)o(3), was isolated from pathogen-infected stele tissue of gossypium barbadense. there are two mol-ecules in the asymmetric unit and the dihedral angle between their naphtho-furan systems is 86.48 (2)°. in the crystal, o-h⋯o hydrogen bonds between the hy-droxy groups and etheric o atoms link the mol-ecules into centrosymmetric tetra-mers. thes ...201323424567
repeated polyploidization of gossypium genomes and the evolution of spinnable cotton fibres.polyploidy often confers emergent properties, such as the higher fibre productivity and quality of tetraploid cottons than diploid cottons bred for the same environments. here we show that an abrupt five- to sixfold ploidy increase approximately 60 million years (myr) ago, and allopolyploidy reuniting divergent gossypium genomes approximately 1-2 myr ago, conferred about 30-36-fold duplication of ancestral angiosperm (flowering plant) genes in elite cottons (gossypium hirsutum and gossypium barb ...201223257886
determination and analysis of the genome sequence of spodoptera littoralis multiple nucleopolyhedrovirus.the spodoptera littoralis multiple nucleopolyhedrovirus (splimnpv), a pathogen of the egyptian cotton leaf worm s. littoralis, was subjected to sequencing of its entire dna genome and bioassay analysis comparing its virulence to that of other baculoviruses. the annotated splimnpv genome of 137,998 bp was found to harbor 132 open reading frames and 15 homologous repeat regions. four unique genes not present in spltmnpv were identified, as were 14 genes that were absent or translocated by comparis ...201323219924
mapping quantitative trait loci for lint yield and fiber quality across environments in a gossypium hirsutum × gossypium barbadense backcross inbred line population.identification of stable quantitative trait loci (qtls) across different environments and mapping populations is a prerequisite for marker-assisted selection (mas) for cotton yield and fiber quality. to construct a genetic linkage map and to identify qtls for fiber quality and yield traits, a backcross inbred line (bil) population of 146 lines was developed from a cross between upland cotton (gossypium hirsutum) and egyptian cotton (gossypium barbadense) through two generations of backcrossing u ...201323064252
contributions of inhalation and dermal exposure to chlorpyrifos dose in egyptian cotton field workers.chlorpyrifos exposures were assessed in 12 egyptian cotton field workers.201323026005
[analysis on linkage inheritance of sub-red plant mutant from upland cotton].analysis of linkage inheritance of sub-red plant, a natural mutant from upland cotton, was conducted by two-point test using parents gossypium barbadense hai7124 and g. hirsutum b026 both with petal spots and green leaves, and g. hirsutum e083 with normal white flowers and, sub-red plants. the average percentage of recombination was 1.35% when calculating test-cross combining populations derived from e083/b026//su9701 and b026/e083//su9701. the average percentage of recombination was 2.94% when ...200919689948
interspecific chromosomal effects on agronomic traits in gossypium hirsutum by ad analysis using intermated g. barbadense chromosome substitution lines.the untapped potential of the beneficial alleles from gossypium barbadense l. has not been well utilized in g. hirsutum l. (often referred to as upland cotton) breeding programs. this is primarily due to genomic incompatibility and technical challenges associated with conventional methods of interspecific introgression. in this study, we used a hypoaneuploid-based chromosome substitution line as a means for systematically introgressing g. barbadense doubled-haploid line '3-79' germplasm into a c ...201322945267
the draft genome of a diploid cotton gossypium raimondii.we have sequenced and assembled a draft genome of g. raimondii, whose progenitor is the putative contributor of the d subgenome to the economically important fiber-producing cotton species gossypium hirsutum and gossypium barbadense. over 73% of the assembled sequences were anchored on 13 g. raimondii chromosomes. the genome contains 40,976 protein-coding genes, with 92.2% of these further confirmed by transcriptome data. evidence of the hexaploidization event shared by the eudicots as well as o ...201222922876
cotton leaf curl multan betasatellite strains cloned from gossypium barbadense further supports selection due to host resistance.the cotton leaf curl multan betasatellite encodes an essential pathogenicity determinant involved in induction of disease symptoms. we have shown recently that a recombinant betasatellite with a satellite conserved region derived from the tomato leaf curl betasatellite, is prevalent in the punjab province and is associated with the breakdown of resistance in cotton to cotton leaf curl disease. we intended to see if the betasatellite that was associated with the first epidemic is still being main ...201222644763
[cloning and functional analysis of chitinase gene gbchi from sea-island cotton (gossypium barbadense)].chitinase is one of the important pathogenesis-related (pr) proteins in plants. by comparative proteomics study, a novel pathogen-responsive chitinase (known as gbchi) has been identified from sea-island cotton (gossypium barbadense). the gbchi cdna was cloned from wilt-resistant sea-island cotton and the anti-fungal activity of the gene product was investigated. qrt-pcr analysis indicated that gbchi was expressed constitutively in root, stem, leaf, flower, and ovule of cotton plant, and the exp ...201222382066
molecular evolution and phylogenetic analysis of genes related to cotton fibers development from wild and domesticated cotton species in gossypium.the domestication of both diploid and tetraploid cotton species was carried out for fiber utilization. to understand the origin and domestication of fibers, 18 genes related to fiber development were individually cloned and sequenced from 22 different cotton species. their structures, phylogenetic relationship and molecular evolution were further studied. in the orthologous and homeologous loci of the 18 genes, the sequence and structure of 72.22% were conserved and 27.78% were diverse. tree top ...201222381639
identification of differentially expressed genes associated with cotton fiber development in a chromosomal substitution line (cs-b22sh).one of the impediments in the genetic improvement of cotton fiber is the paucity of information about genes associated with fiber development. availability of chromosome arm substitution line cs-b22sh (chromosome 22 short arm substitution from 3-79 (gossypium barbadense) into a tm-1 (gossypium hirsutum) background) provides a novel opportunity to study fiber-associated genes because previous studies revealed this line was associated with some superior fiber quality traits compared to tm-1. we us ...200818043952
archaeogenomic evidence of punctuated genome evolution in gossypium.transposable elements (tes) are drivers of evolution resulting in episodic surges of genetic innovation and genomic reorganization (oliver kr, greene wk. 2009. tes: powerful facilitators of evolution. bioessays 31:703-714.), but there is little evidence of the timescale in which this process has occurred (gingerich pd. 2009. rates of evolution. ann rev ecol evol syst. 40:657-675.). the paleontological and archaeological records provide direct evidence for how evolution has proceeded in the past, ...201222334578
effect of exogenous ecdysteroids on growth, development, and fertility of the egyptian cotton leafworm spodoptera littoralis boisd. (lepidoptera: noctuidae). 201617425056
genes expression analyses of sea-island cotton (gossypium barbadense l.) during fiber development.sea-island cotton (gossypium barbadense l.) is one of the most valuable cotton species due to its silkiness, luster, long staples, and high strength, but its fiber development mechanism has not been surveyed comprehensively. we constructed a normalized fiber cdna library (from -2 to 25 dpa) of g. barbadense cv. pima 3-79 (the genetic standard line) by saturation hybridization with genomic dna. we screened pima 3-79 fiber rna from five developmental stages using a cdna array including 9,126 plasm ...200717377794
isolation and characterization of a class iii homeodomain-leucine zipper-like gene from gossypium barbadense.class iii homeodomain-leucine zipper (hd-zip iii) genes are important plant-specific transcription factors which have key roles in different stages of vascular and interfascicular fiber differentiation. a novel hd-zip iii gene, designated gbhb1, was isolated by suppression subtraction hybridization and race (rapid amplification of cdna ends) from gossypium barbadense (sea-island cotton). the gbhb1 cdna has a total length of 3061 bp with an open reading frame of 2508 bp, encoding a predicated pol ...200617343206
chlorpyrifos exposures in egyptian cotton field workers.neurobehavioral deficits have been reported in egyptian pesticide application teams using organophosphorus (op) pesticides, but whether these effects are related to op pesticide exposures has yet to be established. in preparation for a comprehensive study of the relationship between op pesticide dose and neurobehavioral deficits, we assessed exposure within this population. we conducted occupational surveys and workplace observations, and collected air, dermal patch and biological samples from a ...201020193710
overexpression of ghsusa1 increases plant biomass and improves cotton fiber yield and quality.cotton (gossypium spp.) is an important economic crop and the largest source of textile fiber in the world. however, to date, only a few genes have been identified that exhibit critical roles in fiber development, and few has shown positive effects on fiber yield and quality in transgenic cotton. here, we report the characterization of a novel sucrose synthase (susa1) gene from a superior quality fiber germplasm line 7235 in gossypium hirsutum. by association analysis, ghsusa1 was highly correla ...201222044435
modulation of the temporal pattern of calling behavior of female spodoptera littoralis by exposure to sex pheromone.we have examined the timing of calling behavior in the female egyptian cotton leafworm, spodoptera littoralis and its modification by exposure to sex pheromone. the calling rhythm of the female moth was found to be circadian, persistent for at least 4 days once it has been entrained, and could be phase shifted by altering the light:dark regime. we also found that female exposure to pheromone affected the rate and duration of calling. a brief exposure to pheromone gland extract increased the prop ...201222001286
isolation of 6-methoxy gossypol and 6,6'-dimethoxy gossypol from gossypium barbadense sea island cotton.6-methoxy gossypol and 6,6'-dimethoxy gossypol were isolated from the seeds and root bark of a st. vincent sea island variety of cotton (azk-267, grin# pi 528406). crude mixtures of gossypol and the methoxy compounds were obtained by extraction of the tissue with acetone and precipitation with acetic acid. after recrystallization, the preparations were treated with 3-amino-1-propanol to form gossypol schiff's bases, which were separated by preparative reverse phase chromatography. the separated ...200616637683
individual chromosome assignment and chromosomal collinearity in gossypium thurberi, g. trilobum and d subgenome of g. barbadense revealed by bac-fish.the experiment on individual chromosome assignments and chromosomal diversity was conducted using a multi-probe fluorescence in situ hybridization (fish) system in d subgenome of tetraploid gossypium barbadense (d(b)), g. thurberi (d(1)) and g. trilobum (d(8)), which the later two were the possible subgenome donors of tetraploid cottons. the fish probes contained a set of bacterial artificial chromosome (bac) clones specific to 13 individual chromosomes from d subgenome of g. hirsutum (d(h)), a ...201121952206
generation, annotation and analysis of first large-scale expressed sequence tags from developing fiber of gossypium barbadense l.cotton fiber is the world's leading natural fiber used in the manufacture of textiles. gossypium is also the model plant in the study of polyploidization, evolution, cell elongation, cell wall development, and cellulose biosynthesis. g. barbadense l. is an ideal candidate for providing new genetic variations useful to improve fiber quality for its superior properties. however, little is known about fiber development mechanisms of g. barbadense and only a few molecular resources are available in ...201121829504
cleaved aflp (caflp), a modified amplified fragment length polymorphism analysis for certain plant species including cotton (gossypium hirsutum l. or gossypium barbadense l.), the level of amplified fragment length polymorphism (aflp) is relatively low, limiting its utilization in the development of genome-wide linkage maps. we propose the use of frequent restriction enzymes in combination with aflp to cleave the aflp fragments, called cleaved aflp analysis (caflp). using four upland cotton genotypes (g. hirsutum) and three pima cotton (g. barbadense), we demonstrated that ca ...200516133304
ethnomedicine and ethnobotany of fright, a caribbean culture-bound psychiatric syndrome."fright" is an english-speaking caribbean idiom for an illness, or ethnomedical syndrome, of persistent distress. a parallel ethnopsychiatric idiom exists in the french antilles as sésisma. fright is distinct from susto among hispanics, though both develop in the wake of traumatic events. west indian ethnophysiology (ethnoanatomy) theorizes that an overload of stressful emotions (fear, panic, anguish or worry) causes a cold humoral state in which blood coagulates causing prolonged distress and i ...201020163730
occurrence of (+)- and (-)-gossypol in wild species of cotton and in gossypium hirsutum var. marie-galante (watt) hutchinson.gossypol occurs as a mixture of enantiomers in cottonseed. these enantiomers exhibit different biological activities. the (-)-enantiomer is toxic to animals, but it has potential medicinal uses. therefore, cottonseed with >95% (-)-gossypol could have biopharmaceutical applications. the (+)-enantiomer shows little, if any, toxicity to nonruminant animals. thus, cottonseed with >95% (+)-gossypol could be more readily utilized as a feed for nonruminants. the (+)- to (-)-gossypol ratio in commercial ...200516076104
genetic dissection of chromosome substitution lines of cotton to discover novel gossypium barbadense l. alleles for improvement of agronomic traits.we recently released a set of 17 chromosome substitution (cs-b) lines (2n = 52) that contain gossypium barbadense l. doubled-haploid line '3-79' germplasm systematically introgressed into the upland inbred 'tm-1' of g. hirsutum (l.). tm-1 yields much more than 3-79, but cotton from the latter has superior fiber properties. to explore the use of these quasi-isogenic lines in studying gene interactions, we created a partial diallel among six cs-b lines and the inbred tm-1, and characterized their ...201020094702
molecular dissection of interspecific variation between gossypium hirsutum and gossypium barbadense (cotton) by a backcross-self approach: i. fiber elongation.the current study is the first installment of an effort to explore the secondary gene pool for the enhancement of upland cotton (gossypium hirsutum l.) germplasm. we developed advanced-generation backcross populations by first crossing g. hirsutum cv. tamcot 2111 and g. barbadense cv. pima s6, then independently backcrossing f(1) plants to the g. hirsutum parent for three cycles. genome-wide mapping revealed introgressed alleles at an average of 7.3% of loci in each bc(3)f(1) plant, collectively ...200515983756
identification and expression profile of gbagl2, a c-class gene from gossypium agamous (ag)-like gene, gbagl2, was isolated from gossypium barbadense and characterized. alignment and phylogenetic analysis indicated that gbagl2 shared high homology with ag-subfamily genes and belonged to a c-class gene family. dna gel blot analysis showed that gbagl2 belonged to a low-copy gene family. reverse transcriptase-polymerase chain reaction (rt-pcr) and quantitative real-time pcr (qpcr) revealed that gbagl2 was highly expressed in reproductive tissues including ovules and carpel ...200920093747
genotypic and developmental evidence for the role of plasmodesmatal regulation in cotton fiber elongation mediated by callose turnover.cotton fibers are single-celled hairs that elongate to several centimeters long from the seed coat epidermis of the tetraploid species (gossypium hirsutum and gossypium barbadense). thus, cotton fiber is a unique system to study the mechanisms of rapid cell expansion. previous work has shown a transient closure of plasmodesmata during fiber elongation (y.-l. ruan, d.j. llewellyn, r.t. furbank [2001] plant cell 13: 47-60). to examine the importance of this closure in fiber elongation, we compared ...200415557097
functional analysis of gbagl1, a d-lineage gene from cotton (gossypium barbadense).cotton fibres originate from the outer ovule integument and d-lineage genes are essential for ovule development and their roles can be described by the 'abcde' model of flower development. to investigate the role of d-lineage genes during ovule and fibre development, gbagl1 (genbank accession number: fj198049) was isolated from g. barbadense by using the smart race strategy. sequence and phylogenetic analyses revealed that gbagl1 was a member of the d-lineage gene family. southern blot analysis ...201020054032
isolation and characterization of an iaa-responsive gene from gossypium barbadense l.the full-length cdna of an iaa-responsive gene was cloned from gossypium barbadense l. (designated as gbiaa-re) by rapid amplification of cdna ends (race). gbiaa-re gene was 1043-bp long and contained a 573-bp open reading frame encoding a polypeptide of 190 amino acid residues. homology analysis revealed that gbiaa-re strongly resembled known plant iaa-responsive genes. the conserved integrated domain "aux_iaa, aux/iaa family" resided within the region from l11, to v190 of gbiaa-re, and the 4 t ...200415354358
sampling nucleotide diversity in cotton.cultivated cotton is an annual fiber crop derived mainly from two perennial species, gossypium hirsutum l. or upland cotton, and g. barbadense l., extra long-staple fiber pima or egyptian cotton. these two cultivated species are among five allotetraploid species presumably derived monophyletically between g. arboreum and g. raimondii. genomic-based approaches have been hindered by the limited variation within species. yet, population-based methods are being used for genome-wide introgression of ...200919840401
genetic mapping and qtl analysis of fiber-related traits in cotton ( gossypium).cotton, the leading natural fiber crop, is largely produced by two primary cultivated allotetraploid species known as upland or american cotton ( gossypium hirsutum l.) and pima or egyptian cotton ( g. barbadense l.). the allotetraploid species diverged from each other and from their diploid progenitors (a or d genome) through selection and domestication after polyploidization. to analyze cotton ad genomes and dissect agronomic traits, we have developed a genetic map in an f2 population derived ...200414513220
peripheral modulation of olfaction by physiological state in the egyptian leaf worm spodoptera littoralis (lepidoptera: noctuidae).insects show behavioural plasticity based on their physiological state. deprivation from a resource will normally make them more responsive to it or to perform behaviour increasing the probability of encountering such a resource. modulation of the olfactory system has been shown mainly in the central nervous system, but also in the periphery. in this study, antennal sensitivity of females of the egyptian cotton leaf worm spodoptera littoralis to different plant and sex pheromone odours was measu ...200919414013
a combined rflp-ssr-aflp map of tetraploid cotton based on a gossypium hirsutum x gossypium barbadense backcross interspecific gossypium hirsutum x gossypium barbadense backcross population of 75 bc1 plants was evaluated for 1014 markers. the map consists of 888 loci, including 465 aflps, 229 ssrs, 192 rflps, and 2 morphological markers, ordered in 37 linkage groups that represent most if not all of the 26 chromosomes, altogether spanning 4400 cm. loci were not evenly distributed over linkage groups, and 18 of the 26 long groups had a single dense region. this paper proposes a partially revised list of ...200312897870
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