| weedy hosts and prevalence of potential leafhopper vectors (hemiptera: cicadellidae) of a phytoplasma (16srix group) associated with huanglongbing symptoms in citrus groves. | huanglongbing (hlb) is a severe citrus (citrus spp.) disease associated with the bacteria genus candidatus liberibacter, detected in brazil in 2004. another bacterium was found in association with hlb symptoms and characterized as a phytoplasma belonging to the 16srix group. the objectives of this study were to identify potential leafhopper vectors of the hlb-associated phytoplasma and their host plants. leafhoppers were sampled every other week for 12 mo with sticky yellow cards placed at two h ... | 2012 | 22606800 |
| validation and characterization of citrus sinensis micrornas and their target genes. | micrornas play vital role in plant growth and development by changeable expression of their target genes with most plant micrornas having perfect or near-perfect complementarities with their target genes but mirnas in citrus sinensis (csi-mirnas) and their function have not been widely studied. | 2012 | 22583737 |
| carotenoid accumulation in postharvest "cara cara" navel orange (citrus sinensis osbeck) fruits stored at different temperatures was transcriptionally regulated in a tissue-dependent manner. | the main objective of this work was to investigate the effect of storage temperature (4 and 20 °c) on carotenoid accumulation and on the expression levels of seven carotenoid biosynthetic genes (psy, pds, zds, lcyb, lcye, hyb and zep) in postharvest 'cara cara' navel orange (c. sinensis osbeck) fruits. storage at 20 °c rapidly increased the carotenoid content in the peel, whereas the content remained unchanged in the pulp before 35 days of storage. by contrast, storage at 4 °c maintained the car ... | 2012 | 22562781 |
| an integrative analysis of transcriptome and proteome provides new insights into carotenoid biosynthesis and regulation in sweet orange fruits. | an integrative analysis of transcriptome and proteome was performed to identify differential genes/proteins of a red-flesh sweet orange cara cara in comparison with a common cultivar newhall at ripening stages. at the transcript level, gene expression was measured with massively parallel signature sequencing (mpss), and 629 genes of these two sweet orange cultivars differed by two fold or more (fdr<0.001). at the protein level, a combination of 2de and maldi-tof-tof ms identified 48 protein spot ... | 2012 | 22472342 |
| mistletoes and mutant albino shoots on woody plants as mineral nutrient traps. | potassium, sulphur and zinc contents of mistletoe leaves are generally higher than in their hosts. this is attributed to the fact that chemical elements which are cycled between xylem and phloem in the process of phloem loading of sugars are trapped in the mistletoe, because these parasites do not feed their hosts. here it is hypothesized that mutant albino shoots on otherwise green plants should behave similarly, because they lack photosynthesis and thus cannot recycle elements involved in suga ... | 2012 | 22442343 |
| assessment of the genetic diversity of the tunisian citrus rootstock germplasm. | citrus represents a substantial income for farmers in the mediterranean basin. however, the mediterranean citrus industry faces increasing biotic and abiotic constraints. therefore the breeding and selection of new rootstocks are now of the utmost importance. in tunisia, in addition to sour orange, the most widespread traditional rootstock of the mediterranean area, other citrus rootstocks and well adapted to local environmental conditions, are traditionally used and should be important genetic ... | 2012 | 22429788 |
| retrotransposons control fruit-specific, cold-dependent accumulation of anthocyanins in blood oranges. | traditionally, sicilian blood oranges (citrus sinensis) have been associated with cardiovascular health, and consumption has been shown to prevent obesity in mice fed a high-fat diet. despite increasing consumer interest in these health-promoting attributes, production of blood oranges remains unreliable due largely to a dependency on cold for full color formation. we show that sicilian blood orange arose by insertion of a copia-like retrotransposon adjacent to a gene encoding ruby, a myb transc ... | 2012 | 22427337 |
| chemometric characterization of fruit juices from spanish cultivars according to their phenolic compound contents: i. citrus fruits. | the data set composed by phenolic compound profiles of 83 citrus juices (determined by hplc-dad-ms/ms) was evaluated by chemometrics to differentiate them according to citrus species (sweet orange, tangerine, lemon, and grapefruit). cluster analysis (ca) and principal component analysis (pca) showed natural sample grouping among citrus species and even the citrus subclass. most of the information contained in the full data set can be captured if only 15 phenolic compounds (concentration ≥10 mg/l ... | 2012 | 22423971 |
| gene expression in citrus sinensis fruit tissues harvested from huanglongbing-infected trees: comparison with girdled fruit. | distribution of viable candidatus liberibacter asiaticus (calas) in sweet orange fruit and leaves ('hamlin' and 'valencia') and transcriptomic changes associated with huanglongbing (hlb) infection in fruit tissues are reported. viable calas was present in most fruit tissues tested in hlb trees, with the highest titre detected in vascular tissue near the calyx abscission zone. transcriptomic changes associated with hlb infection were analysed in flavedo (ff), vascular tissue (vt), and juice vesic ... | 2012 | 22407645 |
| infrared spectroscopy: a potential tool in huanglongbing and citrus variegated chlorosis diagnosis. | huanglongbing (hlb) and citrus variegated chlorosis (cvc) are serious threats to citrus production and have caused considerable economic losses worldwide, especially in brazil, which is one of the biggest citrus producers in the world. neither disease has a cure nor an efficient means of control. they are also generally confused with each other in the field since they share similar initial symptoms, e.g., yellowing blotchy leaves. the most efficient tool for detecting these diseases is by polyme ... | 2012 | 22365672 |
| unravelling molecular responses to moderate dehydration in harvested fruit of sweet orange (citrus sinensis l. osbeck) using a fruit-specific aba-deficient mutant. | water stress affects many agronomic traits that may be regulated by the phytohormone abscisic acid (aba). within these traits, loss of fruit quality becomes important in many citrus cultivars that develop peel damage in response to dehydration. to study peel dehydration transcriptional responsiveness in harvested citrus fruit and the putative role of aba in this process, this study performed a comparative large-scale transcriptional analysis of water-stressed fruits of the wild-type navelate ora ... | 2012 | 22315241 |
| differential expression of genes involved in alternative glycolytic pathways, phosphorus scavenging and recycling in response to aluminum and phosphorus interactions in citrus roots. | the objective was to determine the possible links between the expression levels of genes involved in alternative glycolytic pathways, phosphorus (p) scavenging and recycling and citrus tolerance to aluminum (al) and/or p-deficiency. 'xuegan' (citrus sinensis) and 'sour pummelo' (citrus grandis) seedlings were irrigated for 18 weeks with nutrient solution containing 0 and 1.2 mm alcl(3)·6h(2)o × 0, 50 and 200 μm kh(2)po(4). c. sinensis displayed more tolerant to al and p-deficiency than c. grandi ... | 2012 | 22307782 |
| melanogenesis of murine melanoma cells induced by hesperetin, a citrus hydrolysate-derived flavonoid. | melanogenesis is a complex process that modulates skin pigmentation to defend photodamage. citrus is the most widely produced fruit crop in the world. people ingest various citrus fruits in their common diets. in the present study, the acid-hydrolyzed and un-hydrolyzed extracts of orange-type citrus fruits were subjected to analyze flavonoid compositions and assess their effects on melanin synthesis in murine b16-f10 melanoma cells. the acid-hydrolyzed extracts of citrus sinensis, c. reticulata, ... | 2012 | 22266363 |
| snp mining in c. clementina bac end sequences; transferability in the citrus genus (rutaceae), phylogenetic inferences and perspectives for genetic mapping. | with the increasing availability of est databases and whole genome sequences, snps have become the most abundant and powerful polymorphic markers. however, snp chip data generally suffers from ascertainment biases caused by the snp discovery and selection process in which a small number of individuals are used as discovery panels. the ongoing international citrus genome consortium sequencing project of the highly heterozygous clementine and sweet orange genomes will soon result in the release of ... | 2012 | 22233093 |
| transcriptome changes during fruit development and ripening of sweet orange (citrus sinensis). | the transcriptome of the fruit pulp of the sweet orange variety anliu (wt) and that of its red fleshed mutant hong anliu (mt) were compared to understand the dynamics and differential expression of genes expressed during fruit development and ripening. | 2012 | 22230690 |
| neurotrophic effect of citrus 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone: promotion of neurite outgrowth via camp/pka/creb pathway in pc12 cells. | 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone (5-oh-hxmf), a hydroxylated polymethoxyflavone, is found exclusively in the citrus genus, particularly in the peels of sweet orange. in this research, we report the first investigation of the neurotrophic effects and mechanism of 5-oh-hxmf in pc12 pheochromocytoma cells. we found that 5-oh-hxmf can effectively induce pc12 neurite outgrowth accompanied with the expression of neuronal differentiation marker protein growth-associated protein-43(gap-43). 5- ... | 2011 | 22140566 |
| insecticidal evaluation of essential oils of citrus sinensis l. (myrtales: myrtaceae) against housefly, musca domestica l. (diptera: muscidae). | the housefly, musca domestica l., is one of the most common insects, associated with vectoring of various etiological agents. in order to search for effective control agent, the essential oil of sweet orange [citrus sinensis (l.) osbeck] was evaluated for its insecticidal activity against the larvae and pupae of housefly using contact toxicity and fumigation bioassays. in the contact toxicity assay, lethal concentration, lc(50) of c. sinensis essential oil against housefly larvae, varied between ... | 2012 | 22127387 |
| cold susceptibility and disinfestation of bactrocera invadens (diptera: tephritidae) in oranges. | to develop a cold disinfestation treatment for the fruit fly bactrocera invadens drew, tsuruta & white (diptera: tephritidae) that is rapidly spreading across africa, research was conducted in nairobi, kenya, using flies from a laboratory culture and 'valencia' orange (citrus sinensis l. osbeck) as the host. the developmental rate of b. invadens in valencia oranges was determined at 28 degrees c, and the third instar was found to be the least susceptible of the egg and larval life stages to cold ... | 2011 | 21882681 |
| a proteomic analysis of the chromoplasts isolated from sweet orange fruits [citrus sinensis (l.) osbeck]. | here, a comprehensive proteomic analysis of the chromoplasts purified from sweet orange using nycodenz density gradient centrifugation is reported. a gelc-ms/ms shotgun approach was used to identify the proteins of pooled chromoplast samples. a total of 493 proteins were identified from purified chromoplasts, of which 418 are putative plastid proteins based on in silico sequence homology and functional analyses. based on the predicted functions of these identified plastid proteins, a large propo ... | 2011 | 21841170 |
| longevity of multiple species of tephritid (diptera) fruit fly parasitoids (hymenoptera: braconidae: opiinae) provided exotic and sympatric-fruit based diets. | while adult parasitic hymenoptera in general feed on floral and extrafloral nectars, hemipteran-honeydews and fluids from punctured hosts, diachasmimorpha longicaudata (ashmead), an old world opiine braconid introduced to tropical/subtropical america for the biological control of anastrepha spp. (tephritidae), can survive on fruit juices as they seep from injured fruit. an ability to exploit fruit juice would allow such a parasitoid to efficiently forage for hosts and food sources simultaneously ... | 2011 | 21839085 |
| citrus essential oil of nigeria. part v: volatile constituents of sweet orange leaf oil (citrus sinensis). | the volatile oils extracted from leaves of eight cultivars of citrus sinensis (l) osbeck were comprehensively analysed by a combination of gc and gc-ms. fifty four constituents accounting for 82.3-98.2% were identified. sabinene (20.9-49.1%), delta-3-carene (0.3-14.3%), (e)-beta-ocimene (4.4-12.6%), linalool (3.7-11.1%) and terpinen-4-ol (1.7-12.5%) were the major constituents that are common to all the volatile oils. in addition, a cluster analysis was carried out and indicated at least four di ... | 2011 | 21815430 |
| cloning, expression, purification and physical and kinetic characterization of the phosphoenolpyruvate carboxylase from orange (citrus sinensis osbeck var. valencia) fruit juice sacs. | phosphoenolpyruvate (pep) carboxylase (pepcase) from orange fruit juice sacs has been cloned and heterogously expressed in high yield. the purified recombinant enzyme displays properties typical of plant pepcase, including activation by sugar phosphates and inhibition by malate and citrate. malate inhibition is weak in the physiological ph range, and the enzyme is also poorly affected by glu and asp, known inhibitors of c(3) plants pepcases. however, it is strongly inhibited by citrate. orange f ... | 2010 | 21802611 |
| fiber concentrate from orange (citrus sinensis l.) bagase: characterization and application as bakery product ingredient. | orange is a tropical fruit used in the juice industry, yielding important quantities of by products. the objective of this work was to obtain a dietary fiber-rich orange bagasse product (dfrobp), evaluate its chemical composition and its use in the preparation of a bakery product (muffin). muffins containing two different levels of dfrobp were studied regarding chemical composition, in vitro starch digestibility, predicted glyceamic index and acceptability in a sensory test. dfrobp showed low fa ... | 2011 | 21731434 |
| partial dehydration and cryopreservation of citrus seeds. | three categories of seed storage behavior are generally recognized among plant species: orthodox, intermediate and recalcitrant. intermediate seeds cannot be stored in liquid nitrogen (ln) without a previous partial dehydration process. the water content (wc) of the seeds at the moment of immersion in ln must be regarded as the most critical factor in cryopreservation. the purpose of this study was to investigate the basis of the optimal hydration status for cryopreservation of citrus seeds: c. ... | 2011 | 21538368 |
| determination of volatile, phenolic, organic acid and sugar components in a turkish cv. dortyol (citrus sinensis l. osbeck) orange juice. | orange flavour is the results of a natural combination of volatile compounds in a well-balanced system including sugars, acids and phenolic compounds. this paper reports the results of the first determination of aroma, organic acids, sugars, and phenolic components in dortyol yerli orange juices. | 2011 | 21480267 |
| photosynthesis down-regulation precedes carbohydrate accumulation under sink limitation in citrus. | photosynthesis down-regulation due to an imbalance between sources and sinks in citrus leaves could be mediated by excessive accumulation of carbohydrates. however, there is limited understanding of the physiological role of soluble and insoluble carbohydrates in photosynthesis regulation and the elements triggering the down-regulation process. in this work, the role of non-structural carbohydrates in the regulation of photosynthesis under a broad spectrum of source-sink relationships has been i ... | 2011 | 21367744 |
| expression analysis in response to low temperature stress in blood oranges: implication of the flavonoid biosynthetic pathway. | the productivity and the geographical distribution of most commercially important citrus varieties are markedly affected by environmental low temperatures. as gene engineering has been shown to be a favourable alternative to produce germplasm with improved cold tolerance, a broad group of cold regulated genes have been previously identified from several citrus spp. by contrast, little information regarding the cold stress response of pigmented sweet orange varieties is available although they mi ... | 2011 | 21349317 |
| quality assessment of industrially processed fruit juices available in dhaka city, bangladesh. | this study was performed to evaluate the nutritional and microbiological quality of industrially processed packed fruit juices of mango (mangifera indica) and orange (citrus sinensis) from nine different manufacturing companies in dhaka city. the highest quantity of total sugar (17.62%) and reducing sugar (9.99%) was recorded in mango juices while the lowest in orange juices (10.41% and 2.24% respectively) of different companies. in this study, protein contents were comparatively higher in mango ... | 2010 | 22691996 |
| stage and tissue-specific modulation of ten conserved mirnas and their targets during somatic embryogenesis of valencia sweet orange. | somatic embryogenesis (se) is a remarkable process of plant somatic cells developing into an embryo capable of forming a complete plant. mirnas play important roles in plant development by regulating expression of their target genes, but its function in se has rarely been studied. herein, ten conserved mirnas with critical functions in plant development are detected by stem-loop qrt-pcr in the se system of valencia sweet orange. sixteen unigenes from citrus are predicted to be targeted by six of ... | 2011 | 21103993 |
| identification of thermolabile pectin methylesterases from sweet orange fruit by peptide mass fingerprinting. | the multiple forms of the enzyme pectin methylesterase (pme) present in citrus fruit tissues vary in activity toward juice cloud-associated pectin substrates and, thus, in their impact on juice cloud stability and product quality. because the proteins responsible for individual pme activities are rarely identified by structural properties or correlated to specific pme genes, matrix-assisted laser desorption-ionization with tandem time-of-flight mass spectrometry (maldi-tof/tof ms) was investigat ... | 2010 | 21053908 |
| gc-ms analysis of headspace and liquid extracts for metabolomic differentiation of citrus huanglongbing and zinc deficiency in leaves of 'valencia' sweet orange from commercial groves. | citrus huanglongbing (hlb) is considered the most destructive citrus disease worldwide. symptoms-based detection of hlb is difficult due to similarities with zinc deficiency. | 2015 | 21046688 |
| characterization of three terpenoid glycosyltransferase genes in 'valencia' sweet orange (citrus sinensis l. osbeck). | three putative terpenoid udp-glycosyltransferase (ugt) genes, designated csugt1, csugt2, and csugt3, were isolated and characterized in 'valencia' sweet orange (citrus sinensis l. osbeck). csugt1 consisted of 1493 nucleotides with an open reading frame encoding 492 amino acids, csugt2 consisted of 1727 nucleotides encoding 504 amino acids, and csugt3 consisted of 1705 nucleotides encoding 468 amino acids. csugt3 had a 145 bp intron at 730-874, whereas csugt1 and csugt2 had none. the three deduce ... | 2010 | 20962888 |
| comprehensive analysis of expressed sequence tags from the pulp of the red mutant 'cara cara' navel orange (citrus sinensis osbeck). | expressed sequence tag (est) analysis of the pulp of the red-fleshed mutant 'cara cara' navel orange provided a starting point for gene discovery and transcriptome survey during citrus fruit maturation. interpretation of the est datasets revealed that the mutant pulp transcriptome held a high section of stress responses related genes, such as the type iii metallothionein-like gene (6.0%), heat shock protein (2.8%), cu/zn superoxide dismutase (0.8%), late embryogenesis abundant protein 5 (0.8%), ... | 2010 | 20883438 |
| synergistic and antagonistic interactions of phenolic compounds found in navel oranges. | phenolic compounds are known to have antioxidant and antimicrobial properties. these properties may be useful in the preservation of foods or beverages. the interactive antioxidant capacity of phenolic compounds within foods has not been well explored. interactions of individual phenolic compounds (chlorogenic acid, hesperidin, luteolin, myricetin, naringenin, p-coumaric acid, and quercetin) at the concentrations found in navel oranges (citrus sinensis) were analyzed for their antioxidant capaci ... | 2010 | 20722912 |
| chemical constituents of citrus sinensis var. shukri from pakistan. | the variety 'shukri' is a new hybrid of citrus sinensis and is frequently grown for its sweet edible fruits in the southern part of pakistan. the leaves of this hybrid variety have been investigated in search of secondary metabolites for the first time. as a result of chromatographic analysis of the methanolic extract, a new ceramide along with a flavonone glycoside and two steroids has been isolated, which were spectroscopically characterized as (e)-n-(1,3,4,5-tetrahydroxyhexadecan-2-yl)dec-4-e ... | 2010 | 20706908 |
| timing of the inhibitory effect of fruit on return bloom of 'valencia' sweet orange (citrus sinensis (l.) osbeck). | in citrus the inhibitory effect of fruit on flower formation is the main cause of alternate bearing. although there are some studies reporting the effect on flowering of the time of fruit removal in a well-defined stage of fruit development, few have investigated the effect throughout the entire fruit growth stage from early fruitlet growth to fruit maturity. the objective of this study was to determine the phenological fruit developmental stage at which the fruit begins its inhibitory effect on ... | 2010 | 20564309 |
| physicochemical and molecular analysis of cell wall metabolism between two navel oranges (citrus sinensis) with different mastication traits. | fj72-1 navel orange and its bud mutant fjwc exhibit differences in melting texture character which are influenced mostly by cell wall metabolism. here we compared the contents of water soluble pectin (wsp), protopectin, total pectin (tp), cellulose, and hemicellulose, activities of polygalaturonase (pg), pectin methylesterase (pme), pectate lyases (pl), cellulase (cel) and gene expression levels of pg, pme, pl and cel between the two cultivars. | 2010 | 20549800 |
| [study on synchronous correlation between fruit characteristic spectrum and the parameter of internal quality for hamlin sweet orange fruit]. | the purpose of our study is to study the relationship between the reflective spectrum of fruit and the internal quality of the orange fruit and find the suitable mark spectrum that can synchronously measure the several fruit quality index at same time to lay a foundation for the development of a rapid and nondestructive field fruit quality analysis technique by analyzing the visible-near infrared spectrum. mature hamlin orange (citrus sinensis (l)cv. hamlin sweet orange) fruits were individually ... | 2010 | 20545159 |
| proteome from lemon fruit flavedo reveals that this tissue produces high amounts of the cit s1 germin-like isoforms. | a multistep procedure has been developed and applied to extract and purify proteins from lemon fruit flavedo. 2de, lc-esi-ms/ms, and bioinformatics were used to detect the high abundance of the germin-like glycoprotein cit s1, a powerful allergen in humans. peptide alignments against citrus est repositories gave the best scores with the c. sinensis cdna (gi|188354270/ey710037), annotated as unknown sweet orange fruit protein; additional blast of peptides against ncbi databases gave high sequence ... | 2010 | 20499937 |
| free and bound cinnamic acid derivatives in corsica sweet blond oranges. | total determination of cinnamic acids (ca), including hydroxycinnamic acid derivatives is generally not accurate since, during hydrolysis, a possible degradation of dihydroxy ca such as caffeic acid could occur. evaluations of ca (ferulic, p-coumaric, sinapic, cinnamic and caffeic acids) before and after hydrolysis have been undertaken using standards and either with or without addition of ascorbic acid and edta. the method was then applied to the determination of free and bound ca in five blond ... | 2010 | 20420324 |
| discovery and comparative profiling of micrornas in a sweet orange red-flesh mutant and its wild type. | red-flesh fruit is absent from common sweet orange varieties, but is more preferred by consumers due to its visual attraction and nutritional properties. our previous researches on a spontaneous red-flesh mutant revealed that the trait is caused by lycopene accumulation and is regulated by both transcriptional and post-transcriptional mechanisms. however, the knowledge on post-transcriptional regulation of lycopene accumulation in fruits is rather limited so far. | 2010 | 20398412 |
| light controls phospholipase a2alpha and beta gene expression in citrus sinensis. | the low-molecular weight secretory phospholipase a2alpha (csspla2alpha) and beta (cspla2beta) cloned in this study exhibited diurnal rhythmicity in leaf tissue of citrus sinensis. only csspla2alpha displayed distinct diurnal patterns in fruit tissues. csspla2alpha and cspla2beta diurnal expression exhibited periods of approximately 24 h; csspla2alpha amplitude averaged 990-fold in the leaf blades from field-grown trees, whereas cspla2beta amplitude averaged 6.4-fold. diurnal oscillation of csspl ... | 2010 | 20388744 |
| influence of different organic fertilizers on quality parameters and the delta(15)n, delta(13)c, delta(2)h, delta(34)s, and delta(18)o values of orange fruit (citrus sinensis l. osbeck). | to investigate the influence of different types of fertilizers on quality parameters, n-containing compounds, and the delta(15)n, delta(13)c, delta(2)h, delta (34)s, and delta(18)o values of citrus fruit, a study was performed on the orange fruit cv. 'valencia late' (citrus sinensis l. osbeck), which was harvested in four plots (three organic and one conventional) located on the same farm. the results demonstrated that different types of organic fertilizers containing the same amount of nitrogen ... | 2010 | 20184327 |
| development of a small scale orange juice extractor. | a small scale motorized orange juice extractor was designed and fabricated, using locally-available construction materials. the essential components of the machine include feeding hopper, top cover, worm shaft, juice sieve, juice collector, waste outlet, transmission belt, main frame, pulleys and bearings. in operation, the worm shaft conveys, crushes, presses and squeezes the fruit to extract the juice. the juice extracted is filtered through the juice sieve into juice collector while the resid ... | 2010 | 23572610 |
| biochemical properties of alpha-amylase from peel of citrus sinensis cv. abosora. | alpha-amylase activity was screened in the peel, as waste fruit, of 13 species and cultivars of egyptian citrus. the species citrus sinensis cv. abosora had the highest activity. alpha-amylase ai from abosora peel was purified to homogeneity using anion and cation-exchange, and gel filtration chromatographies. molecular weight of alpha-amylase ai was found to be 42 kda. the hydrolysis properties of alpha-amylase ai toward different substrates indicated that corn starch is the best substrate. the ... | 2010 | 19941088 |
| use of a custom array to study differentially expressed genes during blood orange (citrus sinensis l. osbeck) ripening. | a flesh-specific oligonucleotide custom array was designed to study gene expression during blood orange ripening. the array included 301 probes derived from a subtracted ssh library, a cdna-aflp collection, and a set of regulatory genes from the harvest citrus database. the custom array was hybridized using samples of moro, a pigmented cultivar, and cadenera, a common cultivar, at three different ripening stages: the immature phase, the halfway point of maturation (corresponding to the start of ... | 2010 | 19864041 |
| comparative proteomics of a lycopene-accumulating mutant reveals the important role of oxidative stress on carotenogenesis in sweet orange (citrus sinensis [l.] osbeck). | a spontaneous sweet orange (citrus sinenesis [l.] osbeck) mutant 'hong anliu' is of high value due to lycopene accumulation in the pulp. in this study, we analyzed the proteomic alterations in the pulp of 'hong anliu' versus its wild type (wt) at four maturing stages by using 2-de combined with maldi-tof-tof ms. among the 74 differentially expressed proteins identified, the majority are predicted to be involved in stress response, carbohydrate/energy metabolism and regulation, or protein fate, m ... | 2009 | 19834898 |
| proteome analysis of citrus sinensis l. (osbeck) flesh at ripening time. | a combination of 2-de and lc-msms approaches was used to identify the differentially expressed proteome of a pigmented sweet orange (citrus sinensis, cv. moro) in comparison with a common cultivar (cadenera) at ripening time. the comparison of the protein patterns of moro and cadenera showed 64 differential expressed protein spots. fifty-five differentially expressed proteins were identified. proteins were classified according to their putative function and known biosynthetic pathways. most of t ... | 2009 | 19775598 |
| identification, recombinant expression, and biochemical characterization of a flavonol 3-o-glucosyltransferase clone from citrus paradisi. | glucosylation is a predominant flavonoid modification reaction affecting the solubility, stability, and subsequent bioavailability of these metabolites. flavonoid glycosides affect taste characteristics in citrus making the associated glucosyltransferases particularly interesting targets for biotechnology applications in these species. in this work, a citrus paradisi glucosyltransferase gene was identified, cloned, and introduced into the pet recombinant protein expression system utilizing prime ... | 2009 | 19733370 |
| phellodendron and citrus extracts benefit joint health in osteoarthritis patients: a pilot, double-blind, placebo-controlled study. | the objective of this clinical study was to assess the potential benefit of a dietary supplement, np 06-1, on joint health in overweight and normal weight adults diagnosed with osteoarthritis. | 2009 | 19682376 |
| seasonal population dynamics of homalodisca vitripennis (hemiptera: cicadellidae) in sweet orange trees maintained under continuous deficit irrigation. | a 2-yr study was conducted in a citrus orchard (citrus sinensis l. osbeck cultivar valencia) to determine the influence of plant water stress on the population dynamics of glassy-winged sharpshooter, homalodisca vitripennis (germar). experimental treatments included irrigation at 100% of the crop evapotranspiration rate (et(c)) and continuous deficit-irrigation regimens at 80 and 60% et(c). microclimate and plant conditions monitored included temperature and humidity in the tree canopy, leaf sur ... | 2009 | 19610409 |
| pattern recognition and genetic algorithms for discrimination of orange juices and reduction of significant components from headspace solid-phase microextraction. | orange (citrus sinensis l.) juice comprises a complex mixture of volatile components that are difficult to identify and quantify. classification and discrimination of the varieties on the basis of the volatile composition could help to guarantee the quality of a juice and to detect possible adulteration of the product. | 2009 | 19609881 |
| thermal degradation kinetics of anthocyanins from blood orange, blackberry, and roselle using the arrhenius, eyring, and ball models. | anthocyanin stability was assessed over temperatures ranging from 30 to 90 degrees c for seven products: blood orange juice [citrus sinensis (l.) osbeck]; two tropical highland blackberry juices (rubus adenotrichus schlech.), one with high content and the other with low content of suspended insoluble solids (sis); and four roselle extracts (hibiscus sabdariffa l.). the blackberry juice showed the highest content of anthocyanins with 1.2 g/l (two times less in the roselle extracts and 12 times le ... | 2009 | 19545116 |
| efficacy of essential oils from edible plants as insecticides against the house fly, musca domestica l. | the compositions of 12 essential oils (eos) obtained by hydrodistillation of edible fruits and herbs were analyzed by gas chromatography/mass spectroscopy (gc/ms). the insecticidal activity of each oil against the house fly musca domestica was evaluated by placing flies in a glass jar with a screw cap that held a piece of eo-treated cotton yarn. the dose necessary to kill 50% of flies (lc(50)) in 30 min was determined at 26 +/- 1 degrees c. twelve eos and 17 individual terpenes were assayed agai ... | 2009 | 19471213 |
| gene isolation and expression analysis of two distinct sweet orange [citrus sinensis l. (osbeck)] tau-type glutathione transferases. | glutathione s-transferases (gsts) represent a multifunctional family of enzymes grouped into four main classes (tau, phi, theta, and zeta) conjugating endobiotic and xenobiotic compounds to glutathione. in plants, this is considered to be a crucial step in the detoxification process as the s-glutathionylated metabolites are tagged for vacuolar sequestration. in this work, we have isolated two glutathione s-transferases belonging to the tau class gsts from sweet orange leaves. the cdna clones con ... | 2009 | 19422890 |
| influence of spray equipment and water volume on coverage of citrus and control of citricola scale, coccus pseudomagnoliarum (hemiptera: coccidae) with mineral oil. | a trial was conducted in a commercial citrus sinensis l. variety 'washington' navel orange orchard to compare the coverage and efficacy against citricola scale coccus pseudomagnoliarum (kuwana) (hemiptera: coccidae) of 45.5 liters/ha of an nc24 agricultural mineral oil treatment applied by two different methods: a conventional air blast sprayer and a rotary atomizer. three water volumes (2,340, 4,680, and 7,020 liters/ha) were applied with the air blast sprayer to determine the optimal spray vol ... | 2009 | 19253648 |
| transcriptome analysis of a spontaneous mutant in sweet orange [citrus sinensis (l.) osbeck] during fruit development. | bud mutations often arise in citrus. the selection of mutants is one of the most important breeding channels in citrus. however, the molecular basis of bud mutation has rarely been studied. to identify differentially expressed genes in a spontaneous sweet orange [c. sinensis (l.) osbeck] bud mutation which causes lycopene accumulation, low citric acid, and high sucrose in fruit, suppression subtractive hybridization and microarray analysis were performed to decipher this bud mutation during frui ... | 2009 | 19218315 |
| estimation of transgene copy number in transformed citrus plants by quantitative multiplex real-time pcr. | quantitative real-time pcr (qrt-pcr) was adapted to estimate transgene copy number of the rice xa21 gene in transgenic citrus plants. this system used taqman qrt-pcr and the endogenous citrus gene encoding for lipid transfer protein (ltp). transgenic "hamlin" sweet orange plants were generated using two different protoplast-gfp transformation systems: cotransformation and single plasmid transformation. a dilution series of genomic dna from one of the transgenic lines was used to generate a stand ... | 2015 | 19194937 |
| hesperidin from citrus sinensis cultivated in dezful, iran. | this study described procedures for extracting and quantitation of hesperidin in the waste orange peel of dezful. two extracting procedure were used. in procedure a hesperidin was isolated from orange peel by extracting the dry peel first with petroleum ether, removing the essential oil and then with methanol. in procedure b alkaline extraction followed by acidification of the extract was used. it was purified by treatment with formamide-activated charcoal. detailed analysis of uv, ir, 1hnmr, 13 ... | 2008 | 19137859 |
| the effects of nitrogen and potassium nutrition on the growth of nonembryogenic and embryogenic tissue of sweet orange (citrus sinensis (l.) osbeck). | mineral nutrients are one of the most basic components of plant tissue culture media. nitrogen in the form of nh4+ and no3(-) is the dominant mineral nutrient in most plant tissue culture formulations, with effects dependent on both the proportion and the amount of nh4+ and no3(-). the effects of nitrogen nutrition on the growth of nonembryogenic and embryogenic cell lines of sweet orange (c. sinensis (l.) osbeck cv. 'valencia'), tissues routinely used in citrus horticultural and plant improveme ... | 2008 | 19087252 |
| ammonium transport and citamt1 expression are regulated by n in citrus plants. | citrus seedlings (citrus sinensis l. osbeck x poncirus trifoliata blanco) were used to describe the effects of different n treatments on the nh4+ influx mediated by high- and low-affinity transport systems (hats and lats, respectively) and citamt1 gene expression. results show that citrus plants favor nh4+ over no3- influx mediated by hats and lats when both n sources are present in the nutrient solution and citrus plants display a much higher capacity to take up nh4+ than no3-. furthermore, nh4 ... | 2009 | 19023591 |
| cloning, characterization and localization of chs gene from blood orange, citrus sinensis (l.) osbeck cv. ruby. | chalcone synthase (chs) is involved in the biosynthesis of anthocyanin. in this study, a full-length dna of chs gene (named as cschs-bo) was cloned from the blood orange, citrus sinensis (l.) osbeck cv. ruby. the gene was 1,512 bp in size containing an open reading frame (1,176 bp) encoding 391 amino acids. comparative and bioinformatic analyses revealed that the deduced protein of cschs-bo was highly homologous to chs from other plant species. the protein of cschs-bo had four chs-specific conse ... | 2009 | 19005777 |
| proteomic analysis of somatic embryogenesis in valencia sweet orange (citrus sinensis osbeck). | two dimensional gel electrophoresis combined with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms) was employed to study the somatic embryogenesis (se) in valencia sweet orange (citrus sinensis osbeck). twenty-four differentially expressed proteins were identified at five time points of citrus se (0, 1, 2, 3, 4 weeks after embryo initiation) covering globular, heart/torpedo and cotyledon-shaped embryo stages. the general expression patterns for these pr ... | 2009 | 18989674 |
| mineral content and botanical origin of spanish honeys. | eleven elements (zn, p, b, mn, mg, cu, ca, ba, sr, na and k) were determined by inductively plasma coupled spectrometry in 40 honey samples from different places of spain and four different botanical origins: eucalyptus (eucalyptus sp.), heather (erica sp.), orange-blossom (citrus sinensis) and rosemary (rosmarinus officinalis). k, ca and p show the higher levels with average concentrations ranged between 434.1-1935mgkg(-1) for k; 42.59-341.0mgkg(-1) for ca and 51.17-154.3mgkg(-1) for p. levels ... | 2005 | 18969853 |
| [study on the solubilization of o/w microemulsion for volatile oil]. | to study the solubilization of o/w microemulsion system for volatile oil. | 2008 | 18831200 |
| the phytochemical analysis and antioxidant activity assessment of orange peel (citrus sinensis) cultivated in greece-crete indicates a new commercial source of hesperidin. | the flavonoid content of several methanolic extract fractions of navel orange peel (flavedo and albedo of citrus sinensis) cultivated in crete (greece) was first analysed phytochemically and then assessed for its antioxidant activity in vitro. the chemical structures of the constituents fractionated were originally determined by comparing their retention times and the obtained uv spectral data with the available bibliographic data and further verified by detailed lc-dad-ms (esi+) analysis. the m ... | 2009 | 18823075 |
| cspldalpha1 and cspldgamma1 are differentially induced during leaf and fruit abscission and diurnally regulated in citrus sinensis. | understanding leaf and fruit abscission is essential in order to develop strategies for controlling the process in fruit crops. mechanisms involved in signalling leaf and fruit abscission upon induction by abscission agents were investigated in citrus sinensis cv. 'valencia'. previous studies have suggested a role for phospholipid signalling; hence, two phospholipase d cdna sequences, cspldalpha1 and cspldgamma1, were isolated and their role was examined. cspldalpha1 expression was reduced in le ... | 2008 | 18799715 |
| survival and development of immature stages of the mediterranean fruit fly (diptera: tephritidae) in citrus fruit. | we studied, under laboratory conditions, the performance of the mediterranean fruit fly, ceratitis capitata (wiedemann) (diptera: tephritidae), immature stages in intact whole fruit of three sweet orange varieties, lemon, and bitter oranges. both citrus variety and fruit part (flavedo, albedo, and pulp) had strong effects on larval performance, smaller effects on pupae, and no effects on eggs. fruit peel was the most critical parameter for larval development and survival, drastically affecting l ... | 2008 | 18613588 |
| characterization of aroma active compounds in fruit juice and peel oil of jinchen sweet orange fruit (citrus sinensis (l.) osbeck) by gc-ms and gc-o. | gas chromatography-mass spectrometry (gc-ms) and gas chromatography-olfactometry (gc-o) were used to determine the aromatic composition and aroma active compounds of fruit juice and peel oil of jinchen sweet orange fruit. totals of 49 and 32 compounds were identified in fruit juice and peel oil, respectively. gc-o was performed to study the aromatic profile of jinchen fruit juice and peel oil. a total of 41 components appeared to contribute to the aroma of fruit juice and peel oil. twelve compon ... | 2008 | 18596659 |
| effects of thermal treatments and storage on pectin methylesterase and peroxidase activity in freshly squeezed orange juice. | a specific indicator of freshness, allowing routine distinction between freshly squeezed orange juices (fsojs) and fsoj-like products, was to be identified. using the actijoule unit of a tubular heater at a flow rate of 60 l/h, fsojs from citrus sinensis (l.) osbeck cv. valencia late were continuously heated on a pilot plant scale at six different temperatures (42-92 degrees c), followed by continuous cooling to ambient temperature and subsequent filling into sterilized glass jars. the cloud sta ... | 2008 | 18582085 |
| [probability distribution of orthezia praelonga douglas (hemiptera: sternorrhyncha: ortheziidae) in citrus]. | the probability distribution of the orthezia scale, orthezia praelonga douglas, was studied in plots of commercial orange orchards (citrus sinensis l., var. "pêra rio"), with 5, 9 and 15 years of age, during one year. the survey of presence or absence of the pest in the plants was carried through twenty samplings in the total area of each plot visually inspecting each plant. the dispersion indexes used were: variance/mean relationship (i), index of morisita (id), coefficient of green (cx) and k ... | 2008 | 18575702 |
| regulation of carotenoid biosynthesis during fruit maturation in the red-fleshed orange mutant cara cara. | cara cara is a spontaneous bud mutation of navel orange (citrus. sinensis l. osbeck) characterized by developing fruits with a pulp of bright red coloration due to the presence of lycopene. peel of mutant fruits is however orange and indistinguishable from its parental. to elucidate the basis of lycopene accumulation in cara cara, we analyzed carotenoid profile and expression of three isoprenoid and nine carotenoid genes in flavedo and pulp of cara cara and navel fruits throughout development an ... | 2008 | 18538806 |
| phellodendron and citrus extracts benefit cardiovascular health in osteoarthritis patients: a double-blind, placebo-controlled pilot study. | the objective of this clinical study was to assess the potential benefit of a dietary supplement, np 06-1, on cardiovascular protective properties in overweight and normal weight adults diagnosed with osteoarthritis of the knee. | 2008 | 18492265 |
| an evaluation of the basis and consequences of a stay-green mutation in the navel negra citrus mutant using transcriptomic and proteomic profiling and metabolite analysis. | a citrus sinensis spontaneous mutant, navel negra (nan), produces fruit with an abnormal brown-colored flavedo during ripening. analysis of pigment composition in the wild-type and nan flavedo suggested that typical ripening-related chlorophyll (chl) degradation, but not carotenoid biosynthesis, was impaired in the mutant, identifying nan as a type c stay-green mutant. nan exhibited normal expression of chl biosynthetic and catabolic genes and chlorophyllase activity but no accumulation of dephy ... | 2008 | 18467459 |
| involvement of polyamine biosynthesis in somatic embryogenesis of valencia sweet orange (citrus sinensis) induced by glycerol. | culture of citrus sinensis embryogenic callus on the embryo-inducing medium (eim) containing glycerol gave rise to a large number of embryos, whereas very few embryos were observed on the callus growth medium (cgm). in the current paper, attempts were made to investigate whether polyamine biosynthesis was involved in glycerol-mediated somatic embryogenesis. quantification of free polyamines by high-performance liquid chromatography showed that the cultures on eim had less putrescine than those o ... | 2009 | 18448195 |
| changes in carotenoid content and biosynthetic gene expression in juice sacs of four orange varieties (citrus sinensis) differing in flesh fruit color. | the contribution of carotenoid composition to the color range of the fruit juice sacs of four orange varieties (citrus sinensis) differing in flesh color, namely, shamouti (normal orange color), sanguinelli ("blood cultivar" purple color), cara cara navel (pink-reddish), and huang pi chen (yellowish color), was investigated. to this end, qualitative and quantitative analyses of carotenoid contents were first performed by high-performance liquid chromatography (hplc) using a c30 column and a phot ... | 2008 | 18433104 |
| preference-performance linkage of the xylem feeding leafhopper, homalodisca vitripennis (hemiptera cicadellidae). | insect species exhibiting a weak linkage between adult preference and immature performance have frequently been shown to be prone to outbreaks. we used choice and no-choice tests to examine the preference-performance linkage of the xylem fluid-feeding leafhopper, homalodisca vitripennis germar. leafhoppers were offered a choice of hosts common to their native range and also a choice from hosts where they have been recently introduced. behavior (residence preference, oviposition preference, and c ... | 2007 | 18284780 |
| [use of morphological and physiological characters, and molecular markers to evaluate the genetic diversity of three clementine cultivars]. | originating from a natural crossing between mandarin and sweet orange at the end of the 19(th) century, clementine diversified through the selection of spontaneous mutations. today, it seems almost impossible to distinguish one variety from another. the development of molecular tools for variety identification is thus necessary. three clementine cultivars, representing distinct groups of fruit maturity, were evaluated. identification criteria were searched at the phenotypical level (organoleptic ... | 2008 | 18187117 |
| a novel bud mutation that confers abnormal patterns of lycopene accumulation in sweet orange fruit (citrus sinensis l. osbeck). | a novel, pleiotropic sweet orange (citrus sinensis l. osbeck) mutant, 'hong anliu', is described. this mutation causes carotenoid accumulation, high sugar, and low acid in the fruits. gas chromatographic analysis revealed that high sugar and low acid in the fruit were caused by the accumulation of sucrose and the deficiency of citric acid. the dominant carotenoid accumulated in albedo, segment membranes, and juice sacs is lycopene, which can reach levels that are a 1000-fold higher than those in ... | 2007 | 18182424 |
| evolving window zone selection method followed by independent component analysis as useful chemometric tools to discriminate between grapefruit juice, orange juice and blends. | this study investigates the use of high resolution 1h nmr as a suitable alternative to the standard chromatographic method for the determination of adulteration of orange juice (citrus sinensis) with grapefruit juice (citrus paradisi) based on flavonoid glycoside content. fifty-nine orange juices (oj), 23 grapefruit juices (gj) and 10 blends (og), obtained from local retail outlets were used to assess the performance of the 1h nmr method. the work presented here introduces the evolving window zo ... | 2007 | 17683731 |
| the effect of gender and ethnicity on children's attitudes and preferences for essential oils: a pilot study. | aromatherapy is frequently recommended for children but children's preferences for specific essential oils are not well documented. | 2016 | 17681258 |
| quality by design: understanding the product variability of a self-nanoemulsified drug delivery system of cyclosporine a. | the objective of this work was to understand the product variability due to size and other characteristics of the snedds by utilizing near infrared (nir) and chemometric analysis, as well as several other well-known procedures. pseudo-ternary phase diagrams were constructed to identify the efficient self-emulsification region using cya solutions in sweet orange oil (oily phase), emulphor el-620 (surfactant), and capmul mcm-c8 (cosurfactant). the formulated snedds were characterized by droplet si ... | 2007 | 17628495 |
| ammonium transport and citamt1 expression are regulated by light and sucrose in citrus plants. | here the isolation and characterization of citamt1 cdna from citrange troyer (citrus sinensis l. osbeckxponcirus trifoliata blanco) is reported, suggesting that this belongs to the amt gene family, which is involved in the high-affinity transport system (hats). results show that in citrus plants, the hats is much more dependent on the light conditions and c status of the roots than the low-affinity transport system. most importantly, a strong correlation was found between the regulation of both ... | 2007 | 17615410 |
| [analysis of constituents of essential oils from fresh and dried pericarp of citrus sinensis by gc-ms]. | to analyze and compare the constituents of the essential oils from the fresh and dried pericarps of citrus sinensis. | 2007 | 17552156 |
| effect of foliar application of micronutrients on the yield and quality of sweet orange (citrus sinensis l.). | an experiment was designed to study the effect of foliar application of micronutrients on the yield, quality and leaf composition of sweet orange, blood red variety at shabazgari, mardan. the experiment was laid out in a randomized complete block design in 2) factorial arrangement. zinc, manganese and boron were applied as foliar spray at the rate of 0.4, 0.2 and 0.04 kg ha(-1), respectively in the presence of 1.56 kg n ha(-1) as urea and 0.4 kg surfactance ha(-1) (as wetting agent) in 400 l of ... | 2007 | 19086544 |
| quality by design: characterization of self-nano-emulsified drug delivery systems (snedds) using ultrasonic resonator technology. | in the present work, a novel application of ultrasonic measurements is detailed to characterize nano-emulsion formulations as a part of the overall quality by design (qbd) goal. ultrasonic resonator technology (urt) was utilized to measure sound velocity and absorption of self-nanoemulsified drug delivery systems (snedds) consisting of various ratios of oil:surfactant:co-surfactant. a qbd concept was used to create different snedds formulations utilizing sweet orange oil (oil), emulphor-620 (sur ... | 2007 | 17521836 |
| csv03-3 is a member of a novel gene family from citrus that encodes a protein with dna binding activity and whose expression is responsive to defense signals and abiotic stress. | we have identified a novel gene designated csv03-3 from citrus sinensis (l.) osbeck that encodes a 50 amino acid polypeptide with a predicted molecular mass of 5.4kda and a pi of 3.7. csv03-3 expression is up-regulated by application of methyl jasmonate, salicylic acid, and abscisic acid as well as by abiotic stress and insect herbivory. csv03-3 belongs to a small gene family consisting of at least three other closely related members (csv03-1, csv03-2, and csv03-4) whose expression is also respo ... | 2008 | 17482713 |
| [effects of foliage spraying sodium bisulfite on photosynthetic physiological characteristics of citrus sinensis with sulphur deficiency]. | the study with solution culture showed that citrus sinensis plants with sulphur deficiency and with sulphur deficiency plus foliage spraying sodium bisulfite ( nahso3 ) halfway had notably decreased photosynthetic pigment content, soluble protein content, net photosynthetic rate (pn), initial fluorescence (fo) , maximum fluorescence (fm) , photochemical efficiency (fv/fm) and electron transmit rate (etr) , compared with those having normal sulphur supply. after 35 days of growth, their photosynt ... | 2007 | 17450735 |
| whole-tree level water balance and its implications on stomatal oscillations in orange trees [citrus sinensis (l.) osbeck] under natural climatic conditions. | sustained cyclic oscillations in stomatal conductance, leaf water potential, and sap flow were observed in young orange trees growing under natural conditions. the oscillations had an average period of approximately 70 min. water uptake by the roots and loss by the leaves was characterized by large time lags which led to imbalances between water supply and demand in the leaves. the bulk of the lag in response between stomatal movements and the upstream water balance resided downstream of the bra ... | 2007 | 17443016 |
| isolation and syntheses of polymethoxyflavones and hydroxylated polymethoxyflavones as inhibitors of hl-60 cell lines. | fifteen polymethoxyflavones (pmfs) and hydroxylated pmfs were isolated from sweet orange (citrus sinensis) peel extract and synthesized to investigate their biological activity. all obtained compounds were tested in hl-60 cancer cell proliferation and apoptosis induction assays. while some pmfs and hydroxylated pmfs had moderate anti-carcinogenic activities, 5-hydroxy-6,7,8,3',4'-pentamethoxyflavone and 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone showed strong inhibitory activities against the pr ... | 2007 | 17391969 |
| binomial sampling to estimate rust mite (acari: eriophyidae) densities on orange fruit. | binomial sampling based on the proportion of samples infested was investigated for estimating mean densities of citrus rust mite, phyllocoptruta oleivora (ashmead), and aculops pelekassi (keifer) (acari: eriophyidae), on oranges, citrus sinensis (l.) osbeck. data for the investigation were obtained by counting the number of motile mites within 600 sample units (each unit a 1-cm2 surface area per fruit) across a 4-ha block of trees (32 blocks total): five areas per 4 ha, five trees per area, 12 f ... | 2007 | 17370833 |
| plant somatic hybrid cytoplasmic dna characterization by single-strand conformation polymorphism. | unlike maternal inheritance in sexual hybridization, plant somatic hybridization allows transfer, mixing and recombination of cytoplasmic genomes. in addition to the use of somatic hybridization in plant breeding programs, application of this unique tool should lead to a better understanding of the roles played by the chloroplastic and mitochondrial genomes in determining agronomically important traits. the nucleotide sequences of cytoplasmic genomes are much more conserved than those of nuclear ... | 2007 | 17331897 |
| [seasonal variation of immature stages of phyllocnistis citrella stainton (lepidoptera: gracillariidae) in citrus sinensis orchards under two management systems]. | phyllocnistis citrella stainton is considered an important pest of citrus, causing both direct (reduction on the photosynthetic area) and indirect damage (facilitation of invasion by bacteria that cause citrus canker). the lack of information about the population dynamics of p. citrella, considering the cultivation systems and varieties grown in citrus orchards in rio grande do sul, brazil, motivated this study. it aimed to evaluate the seasonal variation of immature stages of p. citrella, from ... | 2007 | 17273716 |
| hydraulic characteristics and water relations in pigment-less mutant shoots of an orange tree. | phenotypes more or less deficient in photosynthetic pigments show reduced productivity. not much is known, however, about the influence of pigment-less twigs on the water balance of whole trees. we studied the water relations and hydraulic properties of normal and pigment-less (white) and 1-year-old shoots of 12-year-old citrus sinensis l. trees. compared with green leaves, white leaves showed a pronounced deficiency of pigments, higher stomatal density, the absence of chloroplasts in the guard ... | 2007 | 17241963 |
| expression of phytoene synthase gene (psy) is enhanced during fruit ripening of cara cara navel orange (citrus sinensis osbeck). | citrus is an important fruit crop as regards accumulation of carotenoids. in plant carotenoid biosynthesis, phytoene synthase gene (psy) plays a key role in catalyzing the head-to-head condensation of geranylgeranyl diphosphate molecules to produce colorless phytoene. in the present paper, we reported the phytoene contents determination and characterization of psy during fruit ripening of "washington" navel orange and its red-fleshed mutant "cara cara". results showed that phytoene was exclusive ... | 2007 | 17226057 |
| cire1, a novel transcriptionally active ty1-copia retrotransposon from citrus sinensis. | ltr retrotransposons (ltr-rtns) are widespread constituents of eukaryote genomes, particularly plant genomes. although ltr-rtns from plants were thought to be transcriptionally silent in somatic tissues, evidences of activity under certain conditions are available for some of them. in order to investigate ltr-rtns in the citrus sinensis genome, we analysed them by pcr using degenerate primers corresponding to highly conserved domains. all elements of the two types of ltr-rtn comprise about 23% o ... | 2007 | 17216224 |
| an expedient and versatile protocol for extracting high-quality dna from plant leaves. | introductionprotocols for extracting plant genomic dna have to contend with two major challenges: preventing the oxidation of phenolic substances that can react with nucleic acids and proteins, and eliminating polysaccharides that interfere with downstream enzymatic manipulations of dna. a protocol has been developed in which the above-mentioned substances are removed from the sample before the dna is removed from the chromatin structure. this protocol requires only two tubes during extraction a ... | 2007 | 21357104 |
| quality by design: understanding the formulation variables of a cyclosporine a self-nanoemulsified drug delivery systems by box-behnken design and desirability function. | quality by design (qbd) refers to the achievement of certain predictable quality with desired and predetermined specifications. a very useful component of the qbd is the understanding of factors and their interaction effects by a desired set of experiments. the present project deals with a case study to understand the effect of formulation variables of nanoemulsified particles of a model drug, cyclosporine a (cya). a three-factor, three-level design of experiment (doe) with response surface meth ... | 2007 | 17169518 |
| gene isolation, analysis of expression, and in vitro synthesis of glutathione s-transferase from orange fruit [citrus sinensis l. (osbeck)]. | glutathione s-transferases (gsts) (ec 2.5.1.18) are ubiquitous enzymes that have a defined role in xenobiotic detoxification, but a deeper knowledge of their function in endogenous metabolism is still lacking. in this work, we isolated the cdnas as well as the genomic clones of orange gsts. having considered gene organization and homology data, we suggest that the isolated gst gene is probably involved in the vacuolar import of anthocyanins. we also found that the blood and blond orange gsts sha ... | 2006 | 17117814 |
| molecular and immunological characterization of the glycosylated orange allergen cit s 1. | the ige of sera from patients with a history of allergy to oranges (citrus sinensis) binds a number of proteins in orange extract, including cit s 1, a germin-like protein. in the present study, we have analyzed its immunological cross-reactivity and its molecular nature. sera from many of the patients examined recognize a range of glycoproteins and neoglycoconjugates containing beta1,2-xylose and core alpha1,3-fucose on their n-glycans. these reagents also inhibited the interaction of cit s 1 w ... | 2007 | 17095532 |