| vascular epiphytes and host trees of ant-gardens in an anthropic landscape in southeastern mexico. | ant-gardens (ags) are considered one of the most complex mutualist systems between ants and plants, since interactions involving dispersal, protection, and nutrition occur simultaneously in them; however, little is known about the effects of the transformation of ecosystems on their diversity and interactions. in five environments with different land use within an anthropic landscape in southeastern mexico, we investigated the diversity and composition of epiphytes and host trees of ags built by ... | 2016 | 27866229 |
| draft genome sequence of curtobacterium sp. strain er1/6, an endophytic strain isolated from citrus sinensis with potential to be used as a biocontrol agent. | herein, we report a draft genome sequence of the endophytic curtobacterium sp. strain er1/6, isolated from a surface-sterilized citrus sinensis branch, and it presented the capability to control phytopathogens. functional annotation of the ~3.4-mb genome revealed 3,100 protein-coding genes, with many products related to known ecological and biotechnological aspects of this bacterium. | 2016 | 27856581 |
| ars-media for excel: a spreadsheet tool for calculating media recipes based on ion-specific constraints. | ars-media for excel is an ion solution calculator that uses "microsoft excel" to generate recipes of salts for complex ion mixtures specified by the user. generating salt combinations (recipes) that result in pre-specified target ion values is a linear programming problem. excel's solver add-on solves the linear programming equation to generate a recipe. calculating a mixture of salts to generate exact solutions of complex ionic mixtures is required for at least 2 types of problems- 1) formulati ... | 2016 | 27812202 |
| metabolomic comparative analysis of the phloem sap of curry leaf tree (bergera koenegii), orange jasmine (murraya paniculata), and valencia sweet orange (citrus sinensis) supports their differential responses to huanglongbing. | orange jasmine, murraya paniculata and curry leaf tree, bergera koenegii are alternative hosts for diaphorina citri, the vector of candidatus liberibacter asiaticus (clas), the pathogen of huanglongbing (hlb) in citrus. d. citri feeds on the phloem sap where clas grows. it has been shown that orange jasmine was a better host than curry leaf tree to d. citri. in addition, clas can infect orange jasmine but not curry leaf tree. here, we compared the phloem sap composition of these 2 plants to the ... | 2016 | 27763819 |
| protective effects of sweet orange, unshiu mikan, and mini tomato juice powders on t-bhp-induced oxidative stress in hepg2 cells. | the aim of this study was to investigate the protective effects of juice powders from sweet orange [citrus sinensis (l.) osbeck], unshiu mikan (citrus unshiu marcow), and mini tomato (solanum lycopersicum l.), and their major flavonoids, hesperidin, narirutin, and rutin in tert-butyl hydroperoxide (t-bhp)-induced oxidative stress in hepg2 cells. the increased reactive oxygen species and decreased glutathione levels observed in t-bhp-treated hepg2 cells were ameliorated by pretreatment with juice ... | 2016 | 27752497 |
| comparative analysis of mirnas and their target transcripts between a spontaneous late-ripening sweet orange mutant and its wild-type using small rna and degradome sequencing. | fruit ripening in citrus is not well-understood at the molecular level. knowledge of the regulatory mechanism of citrus fruit ripening at the post-transcriptional level in particular is lacking. here, we comparatively analyzed the mirnas and their target genes in a spontaneous late-ripening mutant, "fengwan" sweet orange (mt) (citrus sinensis l. osbeck), and its wild-type counterpart ("fengjie 72-1," wt). using high-throughput sequencing of small rnas and rna degradome tags, we identified 107 kn ... | 2016 | 27708662 |
| the effect of inhalation of citrus sinensis flowers and mentha spicata leave essential oils on lung function and exercise performance: a quasi-experimental uncontrolled before-and-after study. | recently, there has been an increased interest in the effects of essential oils on athletic performances and other physiological effects. this study aimed to assess the effects of citrus sinensis flower and mentha spicata leaves essential oils inhalation in two different groups of athlete male students on their exercise performance and lung function. | 2017 | 27688737 |
| impact of d-limonene synthase up- or down-regulation on sweet orange fruit and juice odor perception. | citrus fruits are characterized by a complex mixture of volatiles making up their characteristic aromas, being the d-limonene the most abundant one. however, its role on citrus fruit and juice odor is controversial. transgenic oranges engineered for alterations in the presence or concentration of few related chemical groups enable asking precise questions about their contribution to overall odor, either positive or negative, as perceived by the human nose. here, either down- or up-regulation of ... | 2017 | 27664619 |
| the anticonvulsant activity of a flavonoid-rich extract from orange juice involves both nmda and gaba-benzodiazepine receptor complexes. | the usage of dietary supplements and other natural products to treat neurological diseases has been growing over time, and accumulating evidence suggests that flavonoids possess anticonvulsant properties. the aim of this study was to examine the effects of a flavonoid-rich extract from orange juice (oje) in some rodent models of epilepsy and to explore its possible mechanism of action. the genetically audiogenic seizures (ags)-susceptible dba/2 mouse, the pentylenetetrazole (ptz)-induced seizure ... | 2016 | 27657037 |
| yield, esterification degree and molecular weight evaluation of pectins isolated from orange and grapefruit peels under different conditions. | orange (citrus sinensis) and grapefruit (citrus paradise) peels were used as a source of pectin, which was extracted under different conditions. the peels are used under two states: fresh and residual (after essential oil extraction). organic acid (citric acid) and mineral acid (sulfuric acid) were used in the pectin extraction. the aim of this study is the evaluation the effect of extraction conditions on pectin yield, degree of esterification "de" and on molecular weight "mw". results showed t ... | 2016 | 27644093 |
| bioactive compounds in blood oranges (citrus sinensis (l.) osbeck): level and intake. | both the composition and the intake of antioxidants (anthocyanins, ascorbic acid and hydroxycinnamic acids) were reported for all blood oranges including the single cultivars (moro, tarocco and sanguinello) and industrially produced juices. the mean values of the studied bioactive compounds in the edible part oranges were: 9.6mg/100g of orange edible part for the anthocyanins; 8.1, 0.7, 1.3, 3.8, 2.5mg/100g for total hydroxycinnamic acids, caffeic, sinapic, ferulic and coumaric acids, respective ... | 2017 | 27542451 |
| cspao4 of citrus sinensis functions in polyamine terminal catabolism and inhibits plant growth under salt stress. | polyamine oxidase (pao) is a key enzyme catalyzing polyamine catabolism leading to h2o2 production. we previously demonstrated that citrus sinensis contains six putative pao genes, but their functions are not well understood. in this work, we reported functional elucidation of cspao4 in polyamine catabolism and salt stress response. cspao4 was localized to the apoplast and used both spermidine (spd) and spermine (spm) as substrates for terminal catabolism. transgenic plants overexpressing cspao4 ... | 2016 | 27535697 |
| treatment of textile industry effluents using orange waste: a proposal to reduce color and chemical oxygen demand. | various agricultural residues have been tested as biosorbents due to their low cost, high surface area, and favorable surface chemistry. in this work, a sweet orange albedo was tested as a biosorbent for treatment of real textile effluents. the orange albedo powder was prepared by drying the residue at 50 °c and milling to 30 mesh, and then used for dye adsorption from a alkaline (ph = 10.71) effluent. the adsorption process was studied in batch experiments at 30 °c by measuring color removal an ... | 2017 | 27533873 |
| deficit irrigation and maturation stage influence quality and flavonoid composition of 'valencia' orange fruit. | effects of continuous deficit irrigation (di) and partial rootzone drying (prd) treatments (50% etc) in comparison with full irrigation (ci, 100% etc) were investigated during 'valencia' orange fruit maturation. ultra-high-performance liquid chromatography/high-resolution mass spectrometry was used to quantify hesperidin, narirutin, tangeritin, nobiletin, didymin and neoeriocitrin in the fruit juice and peel. | 2017 | 27528197 |
| comparative transcriptome analysis reveals a global insight into molecular processes regulating citrate accumulation in sweet orange (citrus sinensis). | citrate, the predominant organic acid in citrus, determines the taste of these fruits. however, little is known about the synergic molecular processes regulating citrate accumulation. using 'dahongtiancheng' (citrus sinensis) and 'bingtangcheng' (c. sinensis) with significant difference in citrate, the objectives of this study were to understand the global mechanisms of high-citrate accumulation in sweet orange. 'dahongtiancheng' and 'bingtangcheng' exhibit significantly different patterns in ci ... | 2016 | 27507765 |
| identification and transcript profiles of citrus growth-regulating factor genes involved in the regulation of leaf and fruit development. | growth-regulating factor (grf) is an important protein in ga-mediated response, with key roles in plant growth and development. however, it is not known whether or how the grf proteins in citrus to regulate organ size. in this study, nine citrus grf genes (csgrf1-9) were validated from the 'anliu' sweet orange (al, citrus sinensis cv. anliu) by pcr amplification. they all contain two conserved motifs (qlq and wrc) and have 3-4 exons. the transcript levels of genes were detected by qrt-pcr. trans ... | 2016 | 27491940 |
| evaluation of the spatiotemporal dynamics of oxytetracycline and its control effect against citrus huanglongbing via trunk injection. | citrus huanglongbing (hlb) or greening is a devastating bacterial disease that has destroyed millions of trees and is associated with phloem-residing 'candidatus liberibacter asiaticus' (las) in florida. in this study, we evaluated the spatiotemporal dynamics of oxytetracycline in planta and its control effect against hlb via trunk injection. las-infected 'hamlin' sweet orange trees on 'swingle' citrumelo rootstock at the early stage of decline were treated with oxytetracycline hydrochloride (ot ... | 2016 | 27482624 |
| rapid discrimination of bergamot essential oil by paper spray mass spectrometry and chemometric analysis. | a novel approach for the rapid discrimination of bergamot essential oil from other citrus fruits oils is presented. the method was developed using paper spray mass spectrometry (ps-ms) allowing for a rapid molecular profiling coupled with a statistic tool for a precise and reliable discrimination between the bergamot complex matrix and other similar matrices, commonly used for its reconstitution. ambient mass spectrometry possesses the ability to record mass spectra of ordinary samples, in their ... | 2016 | 27460885 |
| aluminum toxicity-induced alterations of leaf proteome in two citrus species differing in aluminum tolerance. | seedlings of aluminum-tolerant 'xuegan' (citrus sinensis) and al-intolerant 'sour pummelo' (citrus grandis) were fertigated for 18 weeks with nutrient solution containing 0 and 1.2 mm alcl₃·6h₂o. al toxicity-induced inhibition of photosynthesis and the decrease of total soluble protein only occurred in c. grandis leaves, demonstrating that c. sinensis had higher al tolerance than c. grandis. using isobaric tags for relative and absolute quantification (itraq), we obtained more al toxicity-respon ... | 2016 | 27455238 |
| enhanced acquisition rates of 'candidatus liberibacter asiaticus' by the asian citrus psyllid (hemiptera: liviidae) in the presence of vegetative flush growth in citrus. | the asian citrus psyllid preferentially feeds and exclusively reproduces on young, newly emerged flush shoots of citrus. asian citrus psyllid nymphs feed and complete their life stages on these flush shoots. recent studies conducted under greenhouse conditions have shown that the transmission rates of 'candidatus liberibacter asiaticus' (clas), the putative causal agent of huanglongbing disease of citrus, are enhanced when flush shoots are present. however, it is unclear if clas acquisition by m ... | 2016 | 27451998 |
| long-term boron-excess-induced alterations of gene profiles in roots of two citrus species differing in boron-tolerance revealed by cdna-aflp. | boron (b) toxicity is observed in some citrus orchards in china. however, limited data are available on the molecular mechanisms of citrus b-toxicity and b-tolerance. using cdna-aflp, we identified 20 up- and 52 down-regulated genes, and 44 up- and 66 down-regulated genes from excess b-treated citrus sinensis and citrus grandis roots, respectively, thereby demonstrating that gene expression profiles were more affected in the latter. in addition, phosphorus and total soluble protein concentration ... | 2016 | 27446128 |
| repellent activity of botanical oils against asian citrus psyllid, diaphorina citri (hemiptera: liviidae). | the asian citrus psyllid, diaphorina citri kuwayama, is the insect vector of the pathogen causing huanglongbing. we selected three botanical oils to evaluate behavioral activity against d. citri. in laboratory olfactometer assays, fir oil was repellent to d. citri females, while litsea and citronella oils elicited no response from d. citri females. in choice settling experiments, d. citri settled almost completely on control plants rather than on plants treated with fir oil at a 9.5 mg/day relea ... | 2016 | 27429006 |
| costs and benefits of insecticide and foliar nutrient applications to huanglongbing-infected citrus trees. | the asian citrus psyllid (acp), diaphorina citri kuwayama (hemiptera: psyllidae), vectors candidatus liberibacter asiaticus, which causes huanglongbing (hlb). in florida, hlb incidence is approaching 100% statewide. yields have decreased and production costs have increased since 2005. despite this, some growers are maintaining a level of production and attribute this in part to aggressive psyllid control and foliar nutrition sprays. however, the value of these practices is debated. a replicated ... | 2017 | 27427170 |
| laser-induced fluorescence spectroscopy (lifs) for discrimination of genetically close sweet orange accessions ( citrus sinensis l. osbeck). | although there is substantial diversity among cultivated sweet oranges genotypes with respect to morphological, physiological, and agronomic traits, very little variation at dna level has been observed. it is possible that this low dna molecular variability is due to a narrow genetic basis commonly observed in this citrus group. the most different morphological characters observed were originated through mutations, which are maintained by vegetative propagation. despite all molecular tools avail ... | 2017 | 27402688 |
| citrate accumulation-related gene expression and/or enzyme activity analysis combined with metabolomics provide a novel insight for an orange mutant. | 'hong anliu' (hal, citrus sinensis cv. hong anliu) is a bud mutant of 'anliu' (al), characterized by a comprehensive metabolite alteration, such as lower accumulation of citrate, high accumulation of lycopene and soluble sugars in fruit juice sacs. due to carboxylic acid metabolism connects other metabolite biosynthesis and/or catabolism networks, we therefore focused analyzing citrate accumulation-related gene expression profiles and/or enzyme activities, along with metabolic fingerprinting bet ... | 2016 | 27385485 |
| assessment of genetic diversity of tunisian orange, citrus sinensis (l.) osbeck using microsatellite (ssr) markers. | citrus are one of the most cultivated crops in the world. economically, they are very important fruit trees in tunisia. little is known about the genetic diversity of the tunisian citrus germplasm. exploring this diversity is a prerequisite for the identification and characterization of the local germplasm to circumvent and controlling genetic erosion caused by biotic and abiotic stress to aid its conservation and use. in the present study, we explored the genetic diversity of 20 tunisian orange ... | 2016 | 27323057 |
| fluorescent carbon nanoparticles from citrus sinensis as efficient sorbents for pollutant dyes. | here, we report a simple, green and economic process for the synthesis of highly fluorescent carbon nanoparticles (cps) through low-temperature carbonization of a fruit waste, citrus sinensis peel. this approach allows the large-scale production of aqueous cps dispersions without any additives and post-treatment processes. the as-prepared cps were of small particle size, exhibited bright blue fluorescence under uv irradiation (λmax = 365 nm) with excellent colloidal stability in water. the chem ... | 2017 | 27321808 |
| identification of potential targets for an anticoagulant pectin. | heparin is a sulfated polysaccharide of animal origin showing excellent anticoagulant properties. although it strongly inhibits the coagulation cascade, its interaction with multiple sites results in several side effects. an ideal alternative compound should not only possess anticoagulant and antithrombotic activities, but also provide specific binding to components of the coagulation cascade to decrease side effects and facilitate the control of pharmacologic actions in patient's body. in this ... | 2017 | 27318178 |
| a simple and rapid hplc-pda ms method for the profiling of citrus peels and traditional italian liquors. | a chromatographic method for the qualitative and quantitative characterization of peels and preparations based on different species of citrus was developed in order to obtain a complete profile of the constituents, including flavonoids and protoalkaloids. commercial peels of sweet orange, lemon, mandarin, and grapefruit were analyzed. seventeen constituents including flavanones, flavones, polymethoxyflavones, and protoalkaloids were identified by hplc-pda, hplc-ms, and hplc-ms/ms using a compari ... | 2016 | 27280933 |
| biolistic transformation of carrizo citrange (citrus sinensis osb. × poncirus trifoliata l. raf.). | the development of transgenic citrus plants by the biolistic method. a protocol for the biolistic transformation of epicotyl explants and transgenic shoot regeneration of immature citrange rootstock, cv. carrizo (citrus sinensis osb. × poncirus trifoliata l. raf.) and plant regeneration is described. immature epicotyl explants were bombarded with a vector containing the nptii selectable marker and the gfp reporter. the number of independent, stably transformed tissues/total number of explants, r ... | 2016 | 27277128 |
| homologues of cslob1 in citrus function as disease susceptibility genes in citrus canker. | the lateral organ boundary domain (lbd) genes encode a group of plant-specific proteins that function as transcription factors in the regulation of plant growth and development. citrus sinensis lateral organ boundary 1 (cslob1) is a member of the lbd family and functions as a disease susceptibility gene in citrus bacterial canker (cbc). thirty-four lbd members have been identified from the citrus sinensis genome. we assessed the potential for additional members of lbd genes in citrus to function ... | 2016 | 27276658 |
| antioxidant activity of oils extracted from orange (citrus sinensis) seeds. | due to the increasing production of food in the world with consequent increase of the production of waste, the importance of developing researches for its use is noticed. thus, the interest in vegetable oils with bioactive compounds, such as the ones extracted from fruit seeds, is growing. therefore, the present study aims to characterize the oils extracted from seeds of hamlin, natal, pera-rio and valencia orange varieties (citrus sinensis), as to the levels of total carotenoids, total phenolic ... | 2016 | 27254458 |
| tetraploidy enhances boron-excess tolerance in carrizo citrange (citrus sinensis l. osb. × poncirus trifoliata l. raf.). | tetraploidy modifies root anatomy which may lead to differentiated capacity to uptake and transport mineral elements. this work provides insights into physiological and molecular characters involved in boron (b) toxicity responses in diploid (2x) and tetraploid (4x) plants of carrizo citrange (citrus sinensis l. osb. × poncirus trifoliata l. raf.), a widely used citrus rootstock. with b excess, 2x plants accumulated more b in leaves than 4x plants, which accounted for their higher b uptake and r ... | 2016 | 27252717 |
| population fluctuation of ceratitis capitata (diptera: tephritidae) as a function of altitude in eastern guatemala. | population fluctuations of ceratitis capitata (wiedemann) were evaluated over a period of 12 mo in four altitudinal strata (400-750, 750-1,100, 1,100-1,450, and 1,450-1,800 meters above sea level, masl) in eastern guatemala. within each altitudinal range, sampling plots were established in coffee plantations and adjacent areas, in which jackson traps were set and baited with trimedlure. coffee berries and other host fruits were collected. population density was lowest at the 400-750 masl stratum ... | 2016 | 27247307 |
| phytohormone profiling of the sweet orange (citrus sinensis (l.) osbeck) leaves and roots using gc-ms-based method. | phytohormones mainly affect plant development and trigger varied responses to biotic and abiotic stresses. the sensitivity of methods used to profile phytohormones is a vital factor that affects the results. we used an improved gc-ms-based method in the selective ion-monitoring (sim) mode to study the phytohormone profiling in citrus tissues. one extraction solvent mixture and two derivatization reagents were used, methyl chloroformate (mcf) and n-methyl-n-(trimethylsilyl) trifluoroacetamide (ms ... | 2016 | 27224902 |
| effect of feeding dried sweet orange (citrus sinensis) peel and lemon grass (cymbopogon citratus) leaves on growth performance, carcass traits, serum metabolites and antioxidant status in broiler during the finisher phase. | the current experiment was conducted to evaluate the effects of feeding dried sweet orange peel (sop) and lemon grass leaves (lgl) as feed additives on broiler growth performance, serum metabolites, and antioxidant status. a total of 192-day-old (ross 308) broiler chickens were distributed randomly into 4 dietary treatments with 4 replicates per each treatment. the dietary treatments included a control diet without any feed additive (t1), a diet containing 0.8 % sop (t2), a diet containing 0.8 % ... | 2016 | 27209636 |
| citap2.10 activation of the terpene synthase cstps1 is associated with the synthesis of (+)-valencene in 'newhall' orange. | aroma is a vital characteristic that determines the quality and commercial value of citrus fruits, and characteristic volatiles have been analyzed in different citrus species. in sweet orange, citrus sinensis, the sesquiterpene (+)-valencene is a key volatile compound in the fruit peel. valencene synthesis is catalyzed by the terpene synthase cstps1, but the transcriptional mechanisms controlling its gene expression are unknown. here, the ap2/erf (apetala2/ethylene response factor) transcription ... | 2016 | 27194737 |
| anti-inflammatory effects of citrus sinensis l., citrus paradisi l. and their combinations. | citrus bioflavonoids embrace a wide group of phenolic compounds effecting the production and scavenging of reactive oxygen species and the processes relating free radical-mediated injury. keeping in view of the antioxidant and anti-inflammatory properties of citrus sinensis and citrus paradisi, present study was undertaken to explore the effects of c. sinensis (orange juice) and c. paradisi (grapefruit juice) at three different doses alone and their two combinations with the objective to examine ... | 2016 | 27166529 |
| antihyperlipidemic effects of citrus sinensis, citrus paradisi, and their combinations. | hyperlipidemia, extensively contributes in the progression of coronary heart diseases and atherosclerosis, but may be managed through alterations in the nutritional pattern. several studies show that diet rich in polyphenols and antioxidants have antiatherogenic effects. citrus sinensis and citrus paradisi are widely known for health benefits and have found to produce antioxidant, anti-inflammatory, and hypolipidemic effects, hence current research was planned to determine the hypolipidemic effe ... | 2017 | 27134462 |
| genome-wide identification of the transcription factors involved in citrus fruit ripening from the transcriptomes of a late-ripening sweet orange mutant and its wild type. | fruit ripening is a genetically programmed process. transcription factors (tfs) play key roles in plant development and ripening by temporarily and spatially regulating the transcription of their target genes. in this study, a total of 159 tfs were identified from a spontaneous late-ripening mutant 'fengwan' (c. sinensis l. osbeck) sweet orange (mt) and its wild-type counterpart ('fengjie 72-1', wt) along the ripening period via the transcription factor prediction of planttfdb 3.0. fifty-two dif ... | 2016 | 27104786 |
| integrated systems biology analysis of transcriptomes reveals candidate genes for acidity control in developing fruits of sweet orange (citrus sinensis l. osbeck). | organic acids, such as citrate and malate, are important contributors for the sensory traits of fleshy fruits. although their biosynthesis has been illustrated, regulatory mechanisms of acid accumulation remain to be dissected. to provide transcriptional architecture and identify candidate genes for citrate accumulation in fruits, we have selected for transcriptome analysis four varieties of sweet orange (citrus sinensis l. osbeck) with varying fruit acidity, succari (acidless), bingtang (low ac ... | 2016 | 27092171 |
| chemistry and pharmacology of citrus sinensis. | presently the search for new drugs from natural resources is of growing interest to the pharmaceutical industry. natural products have been the source of new drugs since ancient times. plants are a good source of secondary metabolites which have been found to have beneficial properties. the present study is a review of the chemistry and pharmacology of citrus sinensis. this review reveals the therapeutic potential of c. sinensis as a source of natural compounds with important activities that are ... | 2016 | 27072414 |
| inhibiting ethylene perception with 1-methylcyclopropene triggers molecular responses aimed to cope with cell toxicity and increased respiration in citrus fruits. | the ethylene perception inhibitor 1-methylcyclopropene (1-mcp) has been critical in understanding the hormone's mode of action. however, 1-mcp may trigger other processes that could vary the interpretation of results related until now to ethylene, which we aim to understand by using transcriptomic analysis. transcriptomic changes in ethylene and 1-mcp-treated 'navelate' (citrus sinensis l. osbeck) oranges were studied in parallel with changes in ethylene production, respiration and peel damage. ... | 2016 | 26990405 |
| [prediction of cadmium content in the leaves of navel orange in heavy metal contaminated soil using vis-nir reflectance spectroscopy]. | visual and near-infrared (vis-nir) reflectance spectroscopy had been used widely in monitoring agricultural pollution in recent years, however, it was rarely applied in monitoring the contamination of heavy metal in orchards. in the present paper, newhall navel orange (citrus sinensis [l.] osbeck cv. newhall) were cultivated in the potted soil contaminated with cadmium (cd) at different levels, and the spectral reflectance and cd content in the leaves were measured simultaneously at different gr ... | 2015 | 26978924 |
| fragile sites of 'valencia' sweet orange (citrus sinensis) chromosomes are related with active 45s rdna. | citrus sinensis chromosomes present a morphological differentiation of bands after staining by the fluorochromes cma and dapi, but there is still little information on its chromosomal characteristics. in this study, the chromosomes in 'valencia' c. sinensis were analyzed by fluorescence in situ hybridization (fish) using telomere dna and the 45s rdna gene as probes combining cma/dapi staining, which showed that there were two fragile sites in sweet orange chromosomes co-localizing at distended 4 ... | 2016 | 26977938 |
| anatomical and physiological responses of citrus trees to varying boron availability are dependent on rootstock. | in citrus, water, nutrient transport and thereby fruit production, are influenced among other factors, by the interaction between rootstock and boron (b) nutrition. this study aimed to investigate how b affects the anatomical structure of roots and leaves as well as leaf gas exchange in sweet orange trees grafted on two contrasting rootstocks in response to b supply. plants grafted on swingle citrumelo or sunki mandarin were grown in a nutrient solution of varying b concentration (deficient, ade ... | 2016 | 26973670 |
| microrna regulatory mechanisms on citrus sinensis leaves to magnesium-deficiency. | magnesium (mg)-deficiency, which affects crop productivity and quality, widespreadly exists in many agricultural crops, including citrus. however, very limited data are available on mg-deficiency-responsive micrornas (mirnas) in higher plants. using illumina sequencing, we isolated 75 (73 known and 2 novel) up- and 71 (64 known and 7 novel) down-regulated mirnas from mg-deficient citrus sinensis leaves. in addition to the remarkable metabolic flexibility as indicated by the great alteration of m ... | 2016 | 26973661 |
| illumina microrna profiles reveal the involvement of mir397a in citrus adaptation to long-term boron toxicity via modulating secondary cell-wall biosynthesis. | the mechanisms underlying tolerance to b-toxicity in plants are still controversial. our previous studies indicated that b-toxicity is mainly limited to leaves in citrus and that alternations of cell-wall structure in vascular bundles are involved in tolerance to b-toxicity. here, mirnas and their expression patterns were first identified in b-treated citrus sinensis (tolerant) and c. grandis (intolerant) leaves via high-throughput sequencing. candidate mirnas were then verified with molecular a ... | 2016 | 26962011 |
| chemistry and pharmacology of citrus sinensis. | presently the search for new drugs from natural resources is of growing interest to the pharmaceutical industry. natural products have been the source of new drugs since ancient times. plants are a good source of secondary metabolites which have been found to have beneficial properties. the present study is a review of the chemistry and pharmacology of citrus sinensis. this review reveals the therapeutic potential of c. sinensis as a source of natural compounds with important activities that are ... | 2016 | 26907240 |
| transmission rates of ‘ca. liberibacter asiaticus’ by asian citrus psyllid are enhanced by the presence and developmental stage of citrus flush. | asian citrus psyllid (diaphorina citri kuwayama) transmits a bacterium ‘candidatus liberibacter asiaticus’ (clas) putatively responsible for a devastating citrus disease known as asiatic huanglongbing (hlb) (citrus greening disease). the psyllid and disease have invaded many citrus-growing regions including the united states, where the disease is seriously jeopardizing the florida citrus industry. we recently concluded research that showed clas transmission rates are increased when citrus flush ... | 2016 | 26884596 |
| the use of laser light to enhance the uptake of foliar-applied substances into citrus (citrus sinensis) leaves. | uptake of foliar-applied substances across the leaf cuticle is central to world food production as well as for physiological investigations into phloem structure and function. yet, despite the presence of stomata, foliar application as a delivery system can be extremely inefficient due to the low permeability of leaf surfaces to polar compounds. | 2016 | 26819863 |
| assessment of navel oranges, clementine tangerines, and rutaceous fruits as hosts of bactrocera cucurbitae and bactrocera latifrons (diptera: tephritidae). | export of citrus spp. fruits may require risk mitigation measures if grown in areas with established tephritid fruit fly (diptera: tephritidae) populations capable of infesting the fruits. the host status of citrus spp. fruits is unclear for two tephritid fruit fly species whose geographic ranges have expanded in recent years: melon fly, bactrocera cucurbitae (cocquillett), and bactrocera latifrons (hendel). in no choice cage infestation studies, b. latifrons oviposited into intact and punctured ... | 2015 | 26816484 |
| regulation of cuticle formation during fruit development and ripening in 'newhall' navel orange (citrus sinensis osbeck) revealed by transcriptomic and metabolomic profiling. | fruit cuticle, which is composed of cutin and wax and biosynthesized during fruit development, plays important roles in the prevention of water loss and the resistance to pathogen infection during fruit development and postharvest storage. however, the key factors and mechanisms regarding the cuticle biosynthesis in citrus fruits are still unclear. here, fruit cuticle of 'newhall' navel orange (citrus sinensis osbeck) was studied from the stage of fruit expansion to postharvest storage from the ... | 2016 | 26795158 |
| hplc-dad phenolic characterization and antioxidant activities of ripe and unripe sweet orange peels. | phenolic compounds of unripe and ripe sweet orange peels were determined using a high-performance liquid chromatography separation method with diode array detector (hplc-dad). the in vitro antioxidant properties and the ec50 (concentration required to obtain a 50% antioxidant effect) values were also determined. the predominant phenolic compounds were quercitrin, rutin, and quercetin with values of 18.77 ± 0.01 mg/ml, 18.65 ± 0.03 mg/ml, and 10.39 ± 0.01 mg/ml respectively in unripe orange peel ... | 2015 | 26783839 |
| selection of reference genes for expression analyses of red-fleshed sweet orange (citrus sinensis). | red-fleshed oranges (citrus sinensis) contain high levels of carotenoids and lycopene. the growing consumer demand for products with health benefits has increased interest in these types of citrus cultivars as a potential source of nutraceuticals. however, little is known about the physiology of these cultivars under brazilian conditions. transcriptome and gene expression analyses are important tools in the breeding and management of red-fleshed sweet orange cultivars. reverse transcription quan ... | 2015 | 26782492 |
| spatio-temporal distribution and natural variation of metabolites in citrus fruits. | to study the natural variation and spatio-temporal accumulation of citrus metabolites, liquid chromatography tandem mass spectrometry (lc-ms) based metabolome analysis was performed on four fruit tissues (flavedo, albedo, segment membrane and juice sacs) and different citrus species (lemon, pummelo and grapefruit, sweet orange and mandarin). using a non-targeted metabolomics approach, more than 2000 metabolite signals were detected, from which more than 54 metabolites, including amino acids, fla ... | 2016 | 26775938 |
| thiamethoxam and imidacloprid drench applications on sweet orange nursery trees disrupt the feeding and settling behaviour of diaphorina citri (hemiptera: liviidae). | chemical control is the method most used for management of diaphorina citri, the vector of the phloem-limited bacteria associated with citrus huanglongbing (hlb) disease. the objectives of this study were to determine the influence of soil-drench applications of neonicotinoids (thiamethoxam and imidacloprid) on the probing behaviour of d. citri on citrus nursery trees, using the electrical penetration graph (epg) technique, and to measure the d. citri settling behaviour after probing on citrus n ... | 2016 | 26694803 |
| identification, characterization and expression analysis of lineage-specific genes within sweet orange (citrus sinensis). | with the availability of rapidly increasing number of genome and transcriptome sequences, lineage-specific genes (lsgs) can be identified and characterized. like other conserved functional genes, lsgs play important roles in biological evolution and functions. | 2015 | 26597278 |
| c- and o-glycosyl flavonoids in sanguinello and tarocco blood orange (citrus sinensis (l.) osbeck) juice: identification and influence on antioxidant properties and acetylcholinesterase activity. | sanguinello and tarocco are the blood orange (citrus sinensis (l.) osbeck) cultivars most diffused worldwide. reversed phase liquid chromatography coupled with ms-ms analysis showed that these two varieties have a similar chromatographic pattern, characterised by the presence of c- and o-glycosyl flavonoids. of the two, sanguinello was found to be far richer in flavonoids than tarocco. in the juices, twelve individual components were identified for the first time, namely, four c-glycosyl flavone ... | 2016 | 26593535 |
| root itraq protein profile analysis of two citrus species differing in aluminum-tolerance in response to long-term aluminum-toxicity. | limited information is available on aluminum (al)-toxicity-responsive proteins in woody plant roots. seedlings of 'xuegan' (citrus sinensis) and 'sour pummelo' (citrus grandis) were treated for 18 weeks with nutrient solution containing 0 (control) or 1.2 mm alcl3 · 6h2o (+al). thereafter, we investigated citrus root protein profiles using isobaric tags for relative and absolute quantification (itraq). the aims of this work were to determine the molecular mechanisms of plants to deal with al-tox ... | 2015 | 26573913 |
| boron-deficiency-responsive micrornas and their targets in citrus sinensis leaves. | micrornas play important roles in the adaptive responses of plants to nutrient deficiencies. most research, however, has focused on nitrogen (n), phosphorus (p), sulfur (s), copper (cu) and iron (fe) deficiencies, limited data are available on the differential expression of mirnas and their target genes in response to deficiencies of other nutrient elements. in this study, we identified the known and novel mirnas as well as the boron (b)-deficiency-responsive mirnas from citrus leaves in order t ... | 2015 | 26538180 |
| composition of citrus sinensis (l.) osbeck cv «maltaise demi-sanguine» juice. a comparison between organic and conventional farming. | juices from conventionally and organically grown citrus sinensis (l.) osbeck cv. maltaise demi-sanguine blood orange were investigated for quality parameters and antioxidant capacity. this blood orange variety is particularly rich in linoleic, linolenic acids, vitamin c and phenolic compounds. the quantitative determination of these compounds in cv. maltaise demi-sanguine juice produced under conventional and organic agricultural practices revealed significant differences. the organically grown ... | 2016 | 26471557 |
| red-fleshed sweet orange juice improves the risk factors for metabolic syndrome. | orange juice consumption can promote lower levels of oxidative stress and inflammation due to the antioxidant activity of citrus flavonoids and carotenoids. in addition, red-fleshed sweet orange juice (red orange juice) also contains lycopene. this study investigated the effects of red orange juice consumption on risk factors for metabolic syndrome. volunteers consumed red orange juice daily for 8 weeks, with clinical and biochemical assessments performed at baseline and on the final day. there ... | 2015 | 26471075 |
| chemopreventive actions of blond and red-fleshed sweet orange juice on the loucy leukemia cell line. | red-fleshed sweet orange juice (roj) comes from a new variety of citrus cultivated in brazil that contains high levels of β-carotene and lycopene, and similar amounts of hesperidin (hsp) and nutrients, equivalently to blond orange juice (boj). such bioactive compounds are associated with chemopreventive actions in several cancer cell lines. the purpose of this study was to examine the cytotoxicity, cell cycle, apoptosis, and cytokine secretion after boj, roj, and hsp treatment of a novel t acute ... | 2015 | 26434864 |
| effect of blue led light intensity on carotenoid accumulation in citrus juice sacs. | in the present study, the effects of blue led light intensity on carotenoid accumulation and expression of genes related to carotenoid biosynthesis were investigated in the juice sacs of satsuma mandarin (citrus unshiu marc.) and valencia orange (citrus sinensis osbeck) in vitro. the results showed that 100 μmol m(-2)s(-1) blue led light (100b) was effective for increasing carotenoid content, especially β-cryptoxanthin, in satsuma mandarin after cultured in vitro for four weeks. in valencia oran ... | 2015 | 26432407 |
| application of sensory evaluation, hs-spme gc-ms, e-nose, and e-tongue for quality detection in citrus fruits. | in this study, electronic tongue (e-tongue), headspace solid-phase microextraction gas chromatography-mass spectrometer (gc-ms), electronic nose (e-nose), and quantitative describe analysis (qda) were applied to describe the 2 types of citrus fruits (satsuma mandarins [citrus unshiu marc.] and sweet oranges [citrus sinensis {l.} osbeck]) and their mixing juices systematically and comprehensively. as some aroma components or some flavor molecules interacted with the whole juice matrix, the change ... | 2015 | 26416698 |
| field evaluation of plant defense inducers for the control of citrus huanglongbing. | huanglongbing (hlb) is currently the most economically devastating disease of citrus worldwide and no established cure is available. defense inducing compounds are able to induce plant resistance effective against various pathogens. in this study the effects of various chemical inducers on hlb diseased citrus were evaluated in four groves (three with sweet orange and one with mandarin) in florida (united states) for two to four consecutive growing seasons. results have demonstrated that plant de ... | 2016 | 26390185 |
| long-term boron-deficiency-responsive genes revealed by cdna-aflp differ between citrus sinensis roots and leaves. | seedlings of citrus sinensis (l.) osbeck were supplied with boron (b)-deficient (without h3bo3) or -sufficient (10 μm h3bo3) nutrient solution for 15 weeks. we identified 54 (38) and 38 (45) up (down)-regulated cdna-aflp bands (transcript-derived fragments, tdfs) from b-deficient leaves and roots, respectively. these tdfs were mainly involved in protein and amino acid metabolism, carbohydrate and energy metabolism, nucleic acid metabolism, cell transport, signal transduction, and stress response ... | 2015 | 26284101 |
| an rna-seq-based reference transcriptome for citrus. | previous rna-seq studies in citrus have been focused on physiological processes relevant to fruit quality and productivity of the major species, especially sweet orange. less attention has been paid to vegetative or reproductive tissues, while most citrus species have never been analysed. in this work, we characterized the transcriptome of vegetative and reproductive tissues from 12 citrus species from all main phylogenetic groups. our aims were to acquire a complete view of the citrus transcrip ... | 2016 | 26261026 |
| genome-wide identification, classification and analysis of hd-zip gene family in citrus, and its potential roles in somatic embryogenesis regulation. | the homeodomain-leucine zipper (hd-zip) transcription factors, which belong to a class of homeobox proteins, has been reported to be involved in different biological processes of plants, including growth and development, photomorphogenesis, flowering, fruit ripening and adaptation responses to environmental stresses. in this study, 27 hd-zip genes (cshbs) were identified in citrus. based on the phylogenetic analysis and characteristics of individual gene or protein, the hd-zip gene family in cit ... | 2015 | 26232336 |
| two-dimensional gel electrophoresis data in support of leaf comparative proteomics of two citrus species differing in boron-tolerance. | here, we provide the data from a comparative proteomics approach used to investigate the response of boron (b)-tolerant 'xuegan' (citrus sinensis) and b-intolerant 'sour pummelo' (citrus grandis) leaves to b-toxicity. using two-dimensional gel electrophoresis (2-de) technique, we identified 50 and 45 protein species with a fold change of more than 1.5 and a p-value of less than 0.05 from b-toxic c. sinensis and c. grandis leaves. these b-toxicity-responsive protein species were mainly involved i ... | 2015 | 26217760 |
| living on the edges: spatial niche occupation of asian citrus psyllid, diaphorina citri kuwayama (hemiptera: liviidae), in citrus groves. | the spatial niche occupation of the asian citrus psyllid, diaphorina citri kuwayama, 1908, was evaluated to determine its field colonization and food resource exploitation strategies in citrus groves. mature grapefruit and sweet orange groves were surveyed as part of an area-wide program in 2009-2010 to determine d. citri population densities and between-tree distribution. in both cultivars, significantly more psyllids were found on perimeter trees throughout the study period suggesting a strong ... | 2015 | 26193111 |
| metabolic changes in citrus leaf volatiles in response to environmental stress. | citrus plants are well known as a rich source of vocs, and several have important roles in defense responses. however, how vocs are regulated in response to environmental stress is not yet well understood. in this study, we investigated dynamic changes of vocs present in leaves of seven citrus species (citrus sinensis, c. limon, c. paradisi, c. unshiu, c. kinokuni, c. grandis, and c. hassaku) in response to mechanical wounding, jasmonic acid (ja), and salicylic acid (sa) as determined by gas chr ... | 2016 | 26188419 |
| analysis of cuticular wax constituents and genes that contribute to the formation of 'glossy newhall', a spontaneous bud mutant from the wild-type 'newhall' navel orange. | navel orange (citrus sinensis [l.] osbeck) fruit surfaces contain substantial quantities of cuticular waxes, which have important eco-physiological roles, such as water retention and pathogen defense. the wax constituents of ripe navel orange have been studied in various reports, while the wax changes occurring during fruit development and the molecular mechanism underlying their biosynthesis/export have not been investigated. recently, we reported a spontaneous bud mutant from the wild-type (wt ... | 2015 | 26177912 |
| changes in the composition of aromatherapeutic citrus oils during evaporation. | the composition of some commercial citrus oils, lemon, sweet orange, and tangerine, designated for aromatherapy, was examined before and after partial evaporation in a stream of nitrogen. the intact oils contained the expected mixtures of mono- and sesquiterpenes, with hydrocarbons dominating and lesser amounts of oxygenated analogues making up the remainder. gas chromatography-mass spectrometry was used to follow alterations in the relative amounts of the various components present as evaporati ... | 2015 | 26161120 |
| bioavailability of β-cryptoxanthin is greater from pasteurized orange juice than from fresh oranges - a randomized cross-over study. | orange fruits and products thereof represent important dietary sources of carotenoids, particularly β-cryptoxanthin. since previous studies reported a positive effect of vegetable processing on carotenoid absorption, our objective was to compare the bioavailability of β-cryptoxanthin from either fresh navel oranges (citrus sinensis l. osbeck) or pasteurized orange juice. | 2015 | 26114420 |
| a comprehensive analysis of chromoplast differentiation reveals complex protein changes associated with plastoglobule biogenesis and remodeling of protein systems in sweet orange flesh. | globular and crystalloid chromoplasts were observed to be region specifically formed in sweet orange (citrus sinensis) flesh and converted from amyloplasts during fruit maturation, which was associated with the composition of specific carotenoids and the expression of carotenogenic genes. subsequent isobaric tag for relative and absolute quantitation (itraq)-based quantitative proteomic analyses of purified plastids from the flesh during chromoplast differentiation and senescence identified 1,38 ... | 2015 | 26056088 |
| structure and mechanism of dimer-monomer transition of a plant poly(a)-binding protein upon rna interaction: insights into its poly(a) tail assembly. | poly(a)-binding proteins (pabps) play crucial roles in mrna biogenesis, stability, transport and translational control in most eukaryotic cells. although animal pabps are well-studied proteins, the biological role, three-dimensional structure and rna-binding mode of plant pabps remain largely uncharacterized. here, we report the structural features and rna-binding mode of a citrus sinensis pabp (cspabpn1). cspabpn1 has a domain architecture of nuclear pabps (pabpns) with a single rna recognition ... | 2015 | 26013164 |
| carotenoid bioaccessibility in pulp and fresh juice from carotenoid-rich sweet oranges and mandarins. | citrus fruits are a good source of carotenoids for the human diet; however, comparative studies of carotenoids in different citrus food matrices are scarce. in this work the concentration and bioaccessibility of carotenoids in sweet oranges and mandarins with marked differences in carotenoid composition were evaluated in pulp and compared to those in fresh juice. the pulp and juice of the red-fleshed cara cara sweet orange variety was highly rich in carotenes (mainly lycopene and phytoene) compa ... | 2015 | 25996796 |
| comparison of microwave, ultrasound and accelerated-assisted solvent extraction for recovery of polyphenols from citrus sinensis peels. | peel of citrus sinensis contains significant amounts of bioactive polyphenols that could be used as ingredients for a number of value-added products with health benefits. extraction of polyphenols from the peels was performed using a microwave-assisted extraction (mae) technique. the effects of aqueous acetone concentration, microwave power, extraction time and solvent-to-solid ratio on the total phenolic content (tpc), total antioxidant activity (taa) (using dpph and orac-values) and individual ... | 2015 | 25977057 |
| variety and harvesting season effects on antioxidant activity and vitamins content of citrus sinensis macfad. | five sweet orange (citrus sinensis osbeck) varieties cultivated in huelva (spain) and picked at two seasons during two consecutive years, were characterized for their antioxidant activity (free radicals scavenging activity, reducing power and lipid peroxidation inhibition) and vitamin content (vitamin e and vitamin c). the effects induced by sweet orange variety and stage of maturity were comprehensively compared by applying 2-way anova and linear discriminant analysis. the results indicated hig ... | 2015 | 25961166 |
| characterisation of free and bound volatile compounds from six different varieties of citrus fruits. | free volatile compounds in six varieties of citrus juices were analyzed by solid-phase microextraction-gas chromatography-mass spectrometry. bound fractions were isolated and extracted with methanol and amberlite xad-2 resin and then hydrolyzed by almond β-glucosidase. a total of 43 free and 17 bound volatile compounds were identified in citrus. free volatile contents in sweet orange were the most abundant, followed by those in grapefruits and mandarins. among free volatiles, terpenes were the m ... | 2015 | 25952837 |
| profiling gene expression in citrus fruit calyx abscission zone (az-c) treated with ethylene. | on-tree storage and harvesting of mature fruit account for a large proportion of cost in the production of citrus, and a reduction of the cost would not be achieved without a thorough understanding of the mechani sm of the mature fruit abscission. genome-wide gene expression changes in ethylene-treated fruit calyx abscission zone (az-c) of citrus sinensis cv. olinda were therefore investigated using a citrus genome array representing up to 33,879 citrus transcripts. in total, 1313 and 1044 diffe ... | 2015 | 25948248 |
| an investigation of boron-toxicity in leaves of two citrus species differing in boron-tolerance using comparative proteomics. | limited data are available on boron (b)-toxicity-responsive proteins in plants. we first applied 2-dimensional electrophoresis (2-de) to compare the effects of b-toxicity on leaf protein profiles in b-tolerant citrus sinensis and b-intolerant citrus grandis seedlings, and identified 27 (20) protein species with increased abundances and 23 (25) protein species with decreased abundances from the former (latter). generally speaking, b-toxicity increased the abundances of protein species involved in ... | 2015 | 25892131 |
| new insights into the evolutionary history of plant sorbitol dehydrogenase. | sorbitol dehydrogenase (sdh, ec 1.1.1.14) is the key enzyme involved in sorbitol metabolism in higher plants. sdh genes in some rosaceae species could be divided into two groups. l-idonate-5-dehydrogenase (lidh, ec 1.1.1.264) is involved in tartaric acid (ta) synthesis in vitis vinifera and is highly homologous to plant sdhs. despite efforts to understand the biological functions of plant sdh, the evolutionary history of plant sdh genes and their phylogenetic relationship with the v. vinifera li ... | 2015 | 25879735 |
| comparative study of flavonoid production in lycopene-accumulated and blonde-flesh sweet oranges (citrus sinensis) during fruit development. | four main flavanone glycosides (fgs) and four main polymethoxylated flavones (pmfs) were determined in fruits of 'cara cara' navel orange, 'seike' navel orange, 'anliu' and 'honganliu' sweet orange (citrus sinensis). no bitter neohesperidosides were detected in the fg profiles, indicating the functional inability of 1,2-rhamnosyltransferase, though relatively high transcription levels were detected in the fruit tissues of 'anliu' and 'honganliu' sweet oranges. different to the fgs, the pmfs only ... | 2015 | 25872450 |
| the state of the art in biosynthesis of anthocyanins and its regulation in pigmented sweet oranges [(citrus sinensis) l. osbeck]. | anthocyanins are water-soluble pigments belonging to the flavonoid compound family involved in nature in several aspects of plant development and defense. by bestowing much of the color and flavor on fruits and vegetables, they are components of the human diet and, thanks to their radical-scavenging properties, are not considered exclusively as food products but also as therapeutic agents. several cultivars of red (or blood) oranges [citrus sinensis (l.) osbeck], such as tarocco, moro, and sangu ... | 2015 | 25871434 |
| genome-wide identification, isolation and expression analysis of auxin response factor (arf) gene family in sweet orange (citrus sinensis). | auxin response factors (arfs) are an important family of proteins in auxin-mediated response, with key roles in various physiological and biochemical processes. to date, a genome-wide overview of the arf gene family in citrus was not available. a systematic analysis of this gene family in citrus was begun by carrying out a genome-wide search for the homologs of arfs. a total of 19 nonredundant arf genes (ciarf) were found and validated from the sweet orange. a comprehensive overview of the ciarf ... | 2015 | 25870601 |
| ultra-high performance liquid chromatography (uhplc) method for the determination of limonene in sweet orange (citrus sinensis) oil: implications for limonene stability. | the citrus-derived bioactive monoterpene limonene is an important industrial commodity and fragrance constituent. an rp isocratic elution c18 ultra-hplc (uhplc) method using a superficially porous stationary phase and photodiode array (pda) detector has been developed for determining the limonene content of sweet orange (citrus sinensis) oil. the method is fast with a cycle time of 1.2 min, linear, precise, accurate, specific, and stability indicating, and it satisfies u.s. pharmacopeia suitabil ... | 2015 | 25857884 |
| alternation of secondary metabolites and quality attributes in valencia orange fruit ( citrus sinensis ) as influenced by storage period and edible covers. | flavonoids (fgs) are a large group of polyphenolic compounds with low molecular weight, found in free and glycozidic forms in plants. citrus fruits can be used as a food supplement containing hesperidin and flavonoids to prevent infections and boost the immune system in human body. the aim of this study was the investigation of the effect of clove oil and storage period on the amount of hesperidin and naringin component in orange peel (cv. valencia). four treatments including clove oil (1 %), wa ... | 2015 | 25829574 |
| tobacco plants over-expressing the sweet orange tau glutathione transferases (csgstus) acquire tolerance to the diphenyl ether herbicide fluorodifen and to salt and drought stresses. | the glutathione transferases (gsts) are members of a superfamily of enzymes with pivotal role in the detoxification of both xenobiotic and endogenous compounds. in this work, the generation and characterization of transgenic tobacco plants over-expressing tau glutathione transferases from citrus sinensis (csgstu1 and csgstu2) and several cross-mutate forms of these genes are reported. putative transformed plants were verified for the presence of the transgenes and the relative quantification of ... | 2015 | 25819876 |
| genome-wide comparative analysis reveals similar types of nbs genes in hybrid citrus sinensis genome and original citrus clementine genome and provides new insights into non-tir nbs genes. | in this study, we identified and compared nucleotide-binding site (nbs) domain-containing genes from three citrus genomes (c. clementina, c. sinensis from usa and c. sinensis from china). phylogenetic analysis of all citrus nbs genes across these three genomes revealed that there are three approximately evenly numbered groups: one group contains the toll-interleukin receptor (tir) domain and two different non-tir groups in which most of proteins contain the coiled coil (cc) domain. motif analysi ... | 2015 | 25811466 |
| rare earth element transfer from soil to navel orange pulp (citrus sinensis osbeck cv. newhall) and the effects on internal fruit quality. | the effects of soil rare earth element (ree) on navel orange quality and safety in rare earth ore areas have gained great attention. this study investigated the transfer characteristics of ree from soil to navel orange pulp (citrus sinensis osbeck cv. newhall) and examined the effects of soil ree on internal fruit quality in xinfeng county, jiangxi province, china. path analysis showed that soil ree, ph, cation exchange capacity (cec), and fe oxide (feox) significantly affected pulp ree concentr ... | 2015 | 25806821 |
| olfactory response of anastrepha striata (diptera: tephritidae) to guava and sweet orange volatiles. | the behavioral responses of virgin and mated female anastrepha striata schiner (diptera: tephritidae) to guava (psidium guajava l.) or sweet orange (citrus sinensis l.) were evaluated separately using multilure traps in two-choice tests in field cages. the results showed that flies were more attracted to guava and sweet orange volatiles than to control (unbaited trap). the physiological state (virgin or mated) of females did not affect their attraction to the fruit volatiles. combined analysis o ... | 2016 | 25800723 |
| de novo transcriptome assembly of pummelo and molecular marker development. | pummelo (citrus grandis) is an important fruit crop worldwide because of its nutritional value. to accelerate the pummelo breeding program, it is essential to obtain extensive genetic information and develop relative molecular markers. here, we obtained a 12-gb transcriptome dataset of pummelo through a mixture of rna from seven tissues using illumina pair-end sequencing, assembled into 57,212 unigenes with an average length of 1010 bp. the annotation and classification results showed that a tot ... | 2015 | 25799271 |
| identification of genes associated with low furanocoumarin content in grapefruit. | some furanocoumarins in grapefruit (citrus paradisi) are associated with the so-called grapefruit juice effect. previous phytochemical quantification and genetic analysis suggested that the synthesis of these furanocoumarins may be controlled by a single gene in the pathway. in this study, cdna-amplified fragment length polymorphism (cdna-aflp) analysis of fruit tissues was performed to identify the candidate gene(s) likely associated with low furanocoumarin content in grapefruit. fifteen tentat ... | 2014 | 25756876 |
| mirnaome analysis associated with anatomic and transcriptomic investigations reveal the polar exhibition of corky split vein in boron deficient citrus sinensis. | corky split vein can develop under long-term boron deficient conditions in citrus sinensis l. osbeck cv. newhall. this symptom only occurs in the upper rather than the lower epidermis of old leaves. our previous study demonstrated that vascular hypertrophy was involved in the symptoms, and the 3rd developmental stage of corky split vein (bd3) was the critical stage for phenotype formation. here, we performed an intensive study on the bd3 vein and its control sample (ck3 vein). a lignin test demo ... | 2015 | 25754997 |
| uptake and distribution of soil applied zinc by citrus trees-addressing fertilizer use efficiency with 68zn labeling. | the zinc (zn) supply increases the fruit yield of citrus trees that are grown, especially in the highly weathered soils of the tropics due to the inherently low nutrient availability in the soil solution. leaf sprays containing micronutrients are commonly applied to orchards, even though the nutrient supply via soil could be of practical value. this study aimed to evaluate the effect of zn fertilizers that are applied to the soil surface on absorption and partitioning of the nutrient by citrus t ... | 2015 | 25751056 |
| isolation and characterization of carotenoid cleavage dioxygenase 4 genes from different citrus species. | in plants, the carotenoid cleavage dioxygenase 4 (ccd4) could target on plastoglobules and cleave specific carotenoids, producing apocarotenoids and volatile compounds. these compounds are important for color and aroma formation in fruits and flowers. in this study, five ccd4 gene members (ccd4a, b, c, d, and e) were investigated in different citrus species including mandarin, pummelo, and sweet orange. sequence analysis showed that the ccd4 genes from all the species examined exhibited extensiv ... | 2015 | 25749981 |
| development and characterization of activated hydrochars from orange peels as potential adsorbents for emerging organic contaminants. | activated hydrochars obtained from the hydrothermal carbonization of orange peels (citrus sinensis) followed by various thermochemical processing were assessed as adsorbents for emerging contaminants in water. thermal activation under flows of co2 or air as well as chemical activation with phosphoric acid were applied to the hydrochars. their characteristics were analyzed and related to their ability to uptake three pharmaceuticals (diclofenac sodium, salicylic acid and flurbiprofen) considered ... | 2015 | 25742754 |