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a draft of the genome and four transcriptomes of a medicinal and pesticidal angiosperm azadirachta indica.the azadirachta indica (neem) tree is a source of a wide number of natural products, including the potent biopesticide azadirachtin. in spite of its widespread applications in agriculture and medicine, the molecular aspects of the biosynthesis of neem terpenoids remain largely unexplored. the current report describes the draft genome and four transcriptomes of a. indica and attempts to contextualise the sequence information in terms of its molecular phylogeny, transcript expression and terpenoid ...201222958331
behavioral responses of schistocerca americana (orthoptera: acrididae) to azadirex (neem)-treated host plants.azadirex (azadirachtin and other biologically active extracts from neem trees) has been shown to have considerable potential to be used in integrated pest management systems based on its growth regulator/insecticide properties. less well known are the antifeedant properties. the feeding-deterrent properties of a commercial azadirex formulation (azatrol ec) were evaluated using both no-choice and choice tests, the american grasshopper, schistocerca americana (drury), and four host plants [savoy c ...200717370818
validated method for phytohormone quantification in plants.phytohormones are long time known as important components of signaling cascades in plant development and plant responses to various abiotic and biotic challenges. quantifications of phytohormone levels in plants are typically carried out using gc or lc-ms/ms systems, due to their high sensitivity, specificity, and the fact that not much sample preparation is needed. however, mass spectrometer-based analyses are often affected by the particular sample type (different matrices), extraction procedu ...201425206356
the effect of citrus-derived oil on bovine blood neutrophil function and gene expression in vitro.research on the use of natural products to treat or prevent microbial invasion as alternatives to antibiotic use is growing. polymorphonuclear leukocytes (pmnl) play a vital role with regard to the innate immune response that affects severity or duration of mastitis. to our knowledge, effect of cold-pressed terpeneless valencia orange oil (tco) on bovine pmnl function has not been elucidated. therefore, the objective of this study was to investigate the effect of tco on bovine blood pmnl chemota ...201525434342
molecular characterization of two glutathione peroxidase genes of panax ginseng and their expression analysis against environmental stresses.glutathione peroxidases (gpxs) are a group of enzymes that protect cells against oxidative damage generated by reactive oxygen species (ros). gpx catalyzes the reduction of hydrogen peroxide (h2o2) or organic hydroperoxides to water or alcohols by reduced glutathione. the presence of gpxs in plants has been reported by several groups, but the roles of individual members of this family in a single plant species have not been studied. two gpx cdnas were isolated and characterized from the embryoge ...201424269671
distribution of 'candidatus liberibacter asiaticus' above and below ground in texas citrus.detection of 'candidatus liberibacter asiaticus' represents one of the most difficult, yet critical, steps of controlling huanglongbing disease. efficient detection relies on understanding the underlying distribution of bacteria within trees. to that end, we studied the distribution of 'ca. l. asiaticus' in leaves of 'rio red' grapefruit trees and in roots of 'valencia' sweet orange trees grafted onto sour orange rootstock. we performed two sets of leaf collection on grapefruit trees; the first ...201627050571
differences in antennal sensillae of male and female peach fruit flies in relation to hosts.antennal sensillae of male and female peach fruit flies, bactrocera zonata (saunders) (diptera: tephritidae), obtained from three different host fruit species (guava, psidium guajava l. (myrtales: myrtaceae); peach, prunus persica (l.) stokes (rosales: rosaceae); and orange, citrus sinensis (l.) osbeck (sapindales: rutaceae)), were studied with scanning electron microscopy. this study was carried out to describe the different types of sensillae present on the three antennal segments (scape, pedi ...201525688086
a new abscisic acid catabolic pathway.we report the discovery of a new hydroxylated abscisic acid (aba) metabolite, found in the course of a mass spectrometric study of aba metabolism in brassica napus siliques. this metabolite reveals a previously unknown catabolic pathway for aba in which the 9'-methyl group of aba is oxidized. analogs of (+)-aba deuterated at the 8'-carbon atom and at both the 8'- and 9'-carbon atoms were fed to green siliques, and extracts containing the deuterated oxidized metabolites were analyzed to determine ...200414671016
production and characterization of films based on blends of chitosan from blue crab (callinectes sapidus) waste and pectin from orange (citrus sinensis osbeck) peel.the objective of this study was to develop and characterize films based on blends of chitosan and pectin, produced in laboratory scale, from industrial wastes. the chitosan was obtained by termoalcaline deacetylation of chitin, extracted from blue crab (callinectes sapidus) waste and characterized according to degree of deacetylation (dd) and viscosimetric molecular weight (mw); and pectin was extracted by conventional heating, from orange (citrus sinensis osbeck) peel and characterized accordin ...201728189792
the antioxidant activity and polyphenolic contents of different plant seeds extracts.different plant seeds extracts of citrus sinensis, hordeum sativum, triticum sativum, canna indica, citrullus vulgaris and capsicum annuum were evaluated for their antioxidant activity by the following methods: 2,2-diphenyl-1-pycril-hydrazyl (dpph) radical scavenging, reducing power, rbcs hemolysis and linoleic acid oxidation, a long with the determination of total phenolic and flavonoids contents. all the methanolic extracts showed high antioxidant activity and have high contents of phenolic an ...200919943462
comparison of the inhibition of monoamine oxidase and butyrylcholinesterase activities by infusions from green tea and some citrus peels.this study sought to investigate the effect of infusions from green tea (camellia sinensis) and some citrus peels [shaddock (citrus maxima), grapefruit (citrus paradisi), and orange (citrus sinensis)] on key enzymes relevant to the management of neurodegenerative conditions [monoamine oxidase (mao) and butyrylcholinesterase (bche)]. the total phenol contents and antioxidant activities as typified by their 2,2(')-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (abts) radicals scavenging abiliti ...201425243093
efficiency and regulation of water transport in some woody and herbaceous species.the efficiency with which plants transport water is related to the water potential differences required to drive water fluxes from the soil to the leaf. a comparative study of two woody and three herbaceous species (citrus sinensis l. cv. koethen, pyrus kawakami l., helianthus annuus l. cv. mammoth russian, capsicum frutescens l. cv. yolo wonder, and sesamum indicum l. cv. glauca) indicated contrasts in water transport efficiency. depression of leaf water potential in response to transpiration i ...197416658853
diurnal variations in photosynthetic products and nitrogen metabolism in expanding leaves.expanding leaves of capsicum frutescens l. cv. california wonder, cucumis melo l. cv. hales best, and citrus sinensis l. osbeck cv. washington navel showed a marked diurnal periodicity in the incorporation of (14)c from photosynthetically fixed (14)co(2) into amino acids. incorporation was virtually nil at the beginning of the photoperiod, reached a maximum in the 6th to 7th hour and decreased during the latter part of the photoperiod. in capsicum frutescens this was apparently a reflection of t ...197316658402
in vitro antiplasmodial activities and synergistic combinations of differential solvent extracts of the polyherbal product, nefang.nefang, a polyherbal product composed of mangifera indica (bark and leaf), psidium guajava, carica papaya, cymbopogon citratus, citrus sinensis, and ocimum gratissimum (leaves), is a potential therapy against p. falciparum malaria. in vitro antiplasmodial activities of its constituent solvent extracts were analyzed on cq-sensitive (3d7) and multidrug resistant (dd2) p. falciparum strains. the interactions involving the differential solvent extracts were further analyzed using a variable potency ...201424877138
antioxidant potential of a polyherbal antimalarial as an indicator of its therapeutic value.nefang is a polyherbal product composed of mangifera indica (bark and leaf), psidium guajava, carica papaya, cymbopogon citratus, citrus sinensis, and ocimum gratissimum (leaves), used for the treatment of malaria. compounds with antioxidant activity are believed to modulate plasmodial infection. antioxidant activity of the constituent aqueous plants extracts, in vitro, was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (dpph), total phenolic content (tpc), and ferric reducing antioxidant pow ...201324454347
preliminary in vitro antisickilng properties of crude juice extracts of persia americana, citrus sinensis, carica papaya and ciklavit®.the antisickling properties of crude juice extracts of the edible portions of three commonly consumed tropical fruits namely persia americana, citrus sinensis, and carica papaya were investigated in vitro alongside a new drug preparation called ciklavit® that has antisickling activity. four different solvent extracts of the crude juice of each fruit including aqueous, acidic, alkaline and alcoholic extracts were prepared and their antisickling effects on sickle cell trait (hbas) and sickle cell ...200921304622
[new directions of research related to chronic wound healing].optimal nutrition, immunological state and psychological condition play an important role in the process of chronic wound healing. infections caused by pathogens resistant to commonly used antibiotics additionally complicate and disturb regeneration of wounds. as part of the treatment, modern wound dressings are used, for example designed on the basis of alginates, dextranomers, hydrogels, hydrofiber, polyurethanes foams, hydrocolloids and liquids for wound debridement such us 0.9% nacl, the pwe ...201324377187
effect of food plants on the volume of repellent secretion obtained in adult zonocerus variegatus (orthoptera: pyrgomorphidae).the volume of secretion obtained from adult zonocerus variegatus (orthoptera: pyrgomorphidae) was influenced by the type of food plants. insects fed on leaves of cassava manihot esculenta, bitter leaves vernonia amygdalina, and a mixture of m. esculenta and acalypha wilkesiana gave a good volume of secretion while chromolaena odorata, elaeis guinensis, aspilia africana and citrus sinensis did not favour secretion production. no significant difference was recorded in the volume of secretion obtai ...200111935920
some aspects of the biology and control using botanicals of the rice moth, corcyra cephalonica (stainton), on some pulses.the life cycle of corcyra cephalonica was studied under ambient laboratory conditions (temperature range 27.5-30 degrees c and 60-73% r.h.) on groundnut, bambara groundnut and cowpea. the mean developmental period ranged from 33.2+/-0.2 to 45.3+/-1.8 days on whole, broken and powdered forms of the food media. egg hatchability was found to be 83%, while adult longevity ranged from 1.5+/-0.5 to 11.9+/-1.3 days for males and 1.5+/-0.5 to 16.5+/-1.2 days for females. sex ratio (male symbol:female sy ...200010758262
identification of flavonoids with trypsin inhibitory activity extracted from orange peel and green tea leaves.orange peel (citrus sinensis) and green tea (camellia sinensis) leaves, rich sources of food flavonoids, were analyzed for their trypsin inhibitory potential. hesperetin, rutin and hesperidin from orange peel, and catechin from green tea leaves, were isolated and their chemical structures were analyzed. all four compounds were evaluated for their trypsin inhibitory potential.201323124490
physical location of the carotenoid biosynthesis genes psy and β-lcy in capsicum annuum (solanaceae) using heterologous probes from citrus sinensis (rutaceae).carotenoids are responsible for a range of fruit colors in different hot pepper (capsicum) varieties, from white to deep red. color traits are genetically determined by three loci, y, c1, and c2, which are associated with carotenogenic genes. although such genes have been localized on genetic maps of capsicum and anchored in lycopersicon and solanum, physical mapping in capsicum has been restricted to only a few clusters of some multiple copy genes. heterologous probes from single copy genes hav ...201121425090
gc-fid/ms profiling of supercritical co2 extracts of peels from citrus aurantium, c. sinensis cv. washington navel, c. sinensis cv. tarocco and c. sinensis cv. doppio sanguigno from dubrovnik area (croatia).the peels of citrus aurantium l. and citrus sinensis osbeck cultivars from the dubrovnik region (south croatia) were extracted by supercritical co2 at 40 degrees c and 10 mpa at 1.76 kg/h to obtain enriched extracts in comparison with simple pressing of the peels. the extracts were analyzed in detail by gas chromatography and mass spectrometry (gc-fid/ms). relevant similarities among the peel oil compositions of c. aurantium and c. sinensis cultivars were found with limonene predominance (up to ...201526411039
anti-hiv-1 activity of eight monofloral iranian honey types.monofloral iranian honeys from eight floral sources were analyzed to determine their anti-hiv-1 activities as well as their effects on lymphocyte proliferation. the peripheral blood mononuclear cells (pbmcs) used in this study were prepared from five healthy volunteers who were seronegative for hiv, hcv, hbv and tb. the anti-hiv-1 activity of eight different honeys was performed by quantitative polymerase chain reaction (pcr) assay and high pure viral nucleic acid kit. the results demonstrated t ...201425333699
to compare the efficacy of two kinds of zhizhu pills in the treatment of functional dyspepsia of spleen-deficiency and qi-stagnation syndrome: a randomized group sequential comparative trial.in traditional chinese medicine (tcm) theory, functional dyspepsia (fd) can be divided into different syndromes according to different clinical symptoms and signs, and the most common one is spleen-deficiency and qi-stagnation syndrome that can be treated by chinese traditional patent medicine--two kinds of zhizhu pills, between which the primary difference in ingredients is that one contains immature orange fruit of citrus aurantium l.(ifca) and the other contains that of citrus sinensis osbeck ...201121762493
pharmacokinetics-based elucidation on disparity in clinical effectiveness between varieties of zhi zhu wan, a traditional chinese medical formula.zhi zhu wan (zzw) is a classical chinese medical formulation used for the treatment of functional dyspepsia that attributed to spleen-deficiency syndrome. zzw contains atractylodes rhizome and fructus citrus immaturus, the later originates from both citrus aurantium l. (bzzw) and citrus sinensis osbeck (rzzw). the present study is designed to elucidate disparities in the clinical efficacy of two zzw varieties based on the pharmacokinetics of naringenin and hesperetin. mehtod: after oral administ ...201020184950
improvement in growth and salt resistance of lemon (citrus limon) trees by an interstock-induced mechanism.interstocks can reduce toxic ion accumulations in leaves of budded citrus trees, but the mechanism is not understood. we grew sour orange (citrus aurantium l.; so) seedlings, budded trees of 'salustiano' orange (citrus sinensis l. osbeck; sao) on so, 'verna' lemon (citrus limon l. burm. f; vl/so) and interstock trees (vl/sao/so) in pots of sand watered with nutrient solution containing 5 (control) or 50 mm nacl (saline treatment) for 12 weeks. plants were harvested in six successive harvests and ...200314532011
influence of aromatherapy on medication administration to residential-care residents with dementia and behavioral challenges.thirteen older persons (seven men and six women) in residential care participated as subjects in this study. all participants had histories of confusion due to dementia and were identified by staff as being consistently resistant to medication administration as indicated by vocal outbursts, moving away, or physical combativeness. subjects were exposed to four aroma interventions during medication administration: 1) lavender vera (lavendula officinalis); 2) sweet orange (citrus aurantium); 3) tea ...201712083347
[two interspecific somatic hybrid plants regenerated via protoplast electro-fusion].protoplasts isolated from cell suspension cultures of 'bonnaza' navel orange (citrus sinensis l. osbeck) were electrically fused with mesophyll protoplasts of rough lemon (citrus jambhiri lush) and goutou orange (citrus aurantium l.) respectively. plants regenerated from both fusion combinations. chromosome counting of randomly selected fifty two globular embryoids as well as all the regenerated seventy four plants from bonnaza navel + rough lemon revealed that twenty six embryoids were tetraplo ...200010976322
relationship between salt tolerance and resistance to polyethylene glycol-induced water stress in cultured citrus cells.salt-tolerant selected cells of shamouti orange (citrus sinensis) and sour orange (citrus aurantium) grew considerably better than nonselected cells at any nacl concentration tested up to 200 millimolar. also, the growth response of each treatment was identical in the two species. however, the performance of cells of the two species under osmotic stress induced by polyethylene glycol (peg), which is presumably a nonabsorbed osmoticum, was significantly different. the nonselected shamouti cell li ...198716665715
evaluation of polyamine and proline levels during low temperature acclimation of citrus.the polyamines (pa) putrescine (put), spermidine (spd), and spermine (spm) were measured during 3 weeks exposure to cold hardening (15.6 degrees c day and 4.4 degrees c night) and nonhardening (32.2 degrees c day and 21.1 degrees c night) temperature regimes in three citrus cultivars: sour orange (so) (citrus aurantium l.), ;valencia' (val) (citrus sinensis l. osbeck), and rough lemon (rl) (citrus jambhiri lush). the changes in pa were compared to the amount of free proline, percent wood kill an ...198716665504
lrr-rlk family from two citrus species: genome-wide identification and evolutionary aspects.leucine-rich repeat receptor-like kinases (lrr-rlks) represent the largest subfamily of plant rlks. the functions of most lrr-rlks have remained undiscovered, and a few that have been experimentally characterized have been shown to have important roles in growth and development as well as in defense responses. although rlk subfamilies have been previously studied in many plants, no comprehensive study has been performed on this gene family in citrus species, which have high economic importance a ...201627515968
the wrky transcription factor family in citrus: valuable and useful candidate genes for citrus breeding.wrky transcription factors belong to a large family of plant transcriptional regulators whose members have been reported to be involved in a wide range of biological roles including plant development, adaptation to environmental constraints and response to several diseases. however, little or poor information is available about wrky's in citrus. the recent release of completely assembled genomes sequences of citrus sinensis and citrus clementina and the availability of ests sequences from other ...201627193354
diversity in sensory quality and determining factors influencing mandarin flavor liking.we evaluated the sensory quality of 42 mandarin varieties that belong to 7 different natural subgroups: common mandarin (citrus reticulata blanco), clementine (citrus clementina hort. ex. tan), satsuma (citrus unshiu marcovitch), mediterranean mandarin (citrus deliciosa tenore), king mandarin (citrus nobilis loureiro), and mandarin hybrids, such as tangor (citrus reticulata × citrus sinensis) and tangelo (citrus reticulata × citrus paradisi). consumer flavor acceptance tests revealed wide divers ...201525559381
citrus and prunuscopia-like retrotransposons.many of the world's most important citrus cultivars ("washington navel", satsumas, clementines) have arisen through somatic mutation. this phenomenon occurs fairly often in the various species and varieties of the genus.the presence of copia-like retrotransposons has been investigated in fruit trees, especially citrus, by using a pcr assay designed to detect copia-like reverse transcriptase (rt) sequences. amplification products from a genotype of each the following species citrus sinensis, citr ...199922665184
resistance of citrus and related genera to diaphorina citri kuwayama (hemiptera: liviidae).the present study was developed to evaluate the resistance of the following genotypes of citrus and related genera to this pest: 'pera,' 'natal', and 'washington navel' oranges (citrus sinensis), 'marsh seedless' grapefruit (citrus paradisi), hardy orange 'rubidoux' (poncirus trifoliata), kumquat (fortunella margarita swingle), citrumelo 'swingle' (c. paradisi x p. trifoliata), and citrange 'troyer' (p. trifoliata x c. sinensis). the experiments were performed in greenhouses with plants grafted ...201427193957
raman spectroscopy an option for the early detection of citrus huanglongbing.this research describes the application of portable field raman spectroscopy combined with a statistical analysis of the resulting spectra, employing principal component analysis (pca) and linear discriminant analysis (lda), in which we determine that this method provides a high degree of reliability in the early detection of huanglongbing (hlb) on sweet orange, disease caused by the bacteria candidatus liberibacter asiaticus. symptomatic and asymptomatic plant samples of sweet orange (citrus si ...201626988658
metabolomic analysis of primary metabolites in citrus leaf during defense responses.mechanical damage is one of the unavoidable environmental stresses to plant growth and development. plants induce a variety of reactions which defend against natural enemies and/or heal the wounded sites. jasmonic acid (ja) and salicylic acid (sa), defense-related plant hormones, are well known to be involved in induction of defense reactions and play important roles as signal molecules. however, defense related metabolites are so numerous and diverse that roles of individual compounds are still ...201727789172
anti-proliferative or cytopathic potential of thapsia garganica, citrus sinensis, citrus limon and vinca rosea extracts against human embryonic kidney carcinoma cell line.developing anti-cancer drugs from natural products is receiving increasing interest worldwide due to limitations and side-effects of anti-cancer drugs. the purpose of this study was to explore the anti-proliferative or cytopathic potential of natural compounds derived from plant sources as alternatives of synthetic compounds on human embryonic kidney carcinoma (hek) cell line.201627618522
the in vitro effect of selected essential oils on the growth and mycotoxin production of aspergillus species.the aim of the present study was to assess the antifungal and anti-toxinogenic activity of 15 essential oils (eos) against three fungi of the genus aspergillus (a. parasiticus kmi-227-lr, a. parasiticus kmi-220-lr and a. flavus kmi-202-lr). the minimum inhibitory doses (mids) of the tested essential oils and their antifungal activity were determined using the micro-atmosphere method. the original commercial essential oil samples of jasminum officinale l., thymus vulgaris l., syzygium aromaticum ...201627322876
development and reproduction of panonychus citri (prostigmata: tetranychidae) on different species and varieties of citrus plants.the species and varieties of citrus plants that are currently grown can favor the population growth of the citrus red mite panonychus citri (mcgregor) (prostigmata: tetranychidae) and alter the pest management programs in citrus groves. in this study we evaluated, in the laboratory, the development and reproduction of p. citri and estimated its life table parameters when reared on four varieties of citrus sinensis (l.) osbeck (valencia, pera, natal, and hamlin), one variety of citrus reticulata ...201526459376
the use of aromasticks at a cancer centre: a retrospective audit.to consider the use of aromasticks in a cancer centre in the uk: the reasons for their use, the choice of essential oils used in them and the demographics of the patients to whom they were given.201425486854
oral dose of citrus peel extracts promotes wound repair in diabetic rats.diabetic patients wound healing is slower than the healthy individuals. three citrus peel extracts; lemon (citrus limon), grapes fruits (citrus paradise) and orange (citrus sinensis) promote wound healing in experimental animals. this study investigated the effect of oral treatment with citrus peel extracts on wound repair of the skin of diabetic rats. the extracts were estimated for vitamin c and total carotenoid contents prior to animal study. diabetes mellitus was induced in rats by intraperi ...201324506007
postharvest control of western flower thrips (thysanoptera: thripidae) and california red scale (hemiptera: diaspididae) with ethyl formate and its impact on citrus fruit quality.the postharvest control of arthropod pests is a challenge that the california citrus industry must overcome when exporting fruit overseas. currently, methyl bromide fumigation is used to control postharvest pests on exported citrus, but it may soon be unavailable because of use restrictions and cost of this health-hazard ozone-depleting chemical. ethyl formate is a natural plant volatile and possible alternative to methyl bromide in postharvest insect control. the objectives of this study were 1 ...201324498732
host susceptibility of citrus cultivars to queensland fruit fly (diptera: tephritidae).citrus crops are considered to be relatively poor hosts for queensland fruit fly, bactrocera tryoni (froggatt), as for other tephritid species. australian citrus growers and crop consultants have reported observable differences in susceptibility of different citrus cultivars under commercial growing conditions. in this study we conducted laboratory tests and field surveys to determine susceptibility to b. tryoni of six citrus cultivars [(eureka lemon (citrus limon (l.) osbeck); navel and valenci ...201323786078
green synthesized silver nanoparticles for selective colorimetric sensing of hg2+ in aqueous solution at wide ph range.in the present study, the syntheses of crystalline silver and gold nanoparticles (nps) has been demonstrated in a green and environmentally friendly approach using citrus fruit extracts (lemon, citrus limon (cl-1) and sweet orange, citrus limetta (cl-2)). in addition, potentially hazardous metal ion sensing properties of these nps in aqueous solution has been explored. cl-1 and cl-2 that predominantly contained citric and ascorbic acid, exhibited different reducing abilities towards silver and g ...201323741735
green synthesis of gold nanoparticles using citrus fruits (citrus limon, citrus reticulata and citrus sinensis) aqueous extract and its characterization.this study reports the biological synthesis of gold nanoparticles by the reduction of haucl(4) by using citrus fruits (citrus limon, citrus reticulata and citrus sinensis) juice extract as the reducing and stabilizing agent. a various shape and size of gold nanoparticles were formed when the ratio of the reactants were altered with respect to 1.0mm chloroauric acid solution. the gold nanoparticles obtained were characterized by uv-visible spectra, transmission electron microscopy (tem) and x-ray ...201323211617
volatile constituents of wild citrus mangshanyegan (citrus nobilis lauriro) peel oil.volatiles of a wild mandarin, mangshanyegan (citrus nobilis lauriro), were characterized by gc-ms, and their aroma active compounds were identified by aroma extract dilution analysis (aeda) and gas chromatography-olfactometry (gc-o). the volatile profile of mangshanyegan was compared with those of other four citrus species, kaopan pummelo (citrus grandis), eureka lemon (citrus limon), huangyanbendizao tangerine (citrus reticulata), and seike navel orange (citrus sinensis). monoterpene hydrocarbo ...201222352344
preference and performance of phyllocnistis citrella stainton (lepidoptera: gracillariidae) on three citrus hosts: laboratory and field assessment.the relationship between preference and performance is crucial to the ecology and evolution of plant-insect interactions. oviposition preference and offspring performance were evaluated for a citrus pest, the leafminer phyllocnistis citrella (lepidoptera: gracillariidae), on three of its host plants: lemon (citrus limon l. burm.), orange (citrus sinensis l. osbeck), and grapefruit (citrus paradisi macfadyen) in tucumán province (northwest argentina). choice and no-choice tests were performed in ...200818801269
polyphenols distribution in juices from citrus allotetraploid somatic hybrids and their sexual hybrids.the polyphenol profile of an interspecific allotetraploid somatic hybrid, achieved by protoplast fusion, the 'valencia' sweet orange ( citrus sinensis l. osbeck) + 'femminello' lemon ( citrus limon l. burm), and three sexual hybrids obtained by backcrosses between femminello lemon and the allotetraploid somatic hybrid ('valencia' + 'femminello') was studied by liquid chromatography-ultraviolet-diode array detector-mass spectrometry (lc-uv-dad-ms). the aim of the work was to evaluate whether supe ...200717914878
interstock-induced mechanism of increased growth and salt resistance of orange (citrus sinensis) trees.interstocks improve the growth and salt resistance of lemon (citrus limon (l.) burm. f.) trees, but their effects on orange (citrus sinensis (l.) osbeck) trees are unknown. we grew 'cleopatra' mandarin (cm) seedlings, budded trees of 'salustiano' orange (sao) on cm, 'valencia late' orange (vlo) on cm (vlo/cm), and interstock trees vlo/sao/cm in pots of sand watered with nutrient solution containing 5 (control) or 50 mm nacl for 12 weeks. plants were harvested on six successive occasions and the ...200415294757
effects of fruit juices of citrus sinensis l. and citrus limon l. on experimental hypercholesterolemia in the rat.in citrus juices there are various substances of different chemical nature, some of which possess already-known hypocholesterolemic properties. therefore it has seemed interesting to evaluate the effects of a prolonged administration of lyophilized juices of citrus sinensis l. osbeck and citrus limon l. burm fil. in diet-induced rat hypercholesterolemia. lyophilized citrus juices were administered for 15 days (by gavage, at the dose of 5g/kg in aqueous veicle in a volume of 0.5 ml/100 g body wei ...199623194620
complete chloroplast genome sequence of omani lime (citrus aurantiifolia) and comparative analysis within the rosids.the genus citrus contains many economically important fruits that are grown worldwide for their high nutritional and medicinal value. due to frequent hybridizations among species and cultivars, the exact number of natural species and the taxonomic relationships within this genus are unclear. to compare the differences between the citrus chloroplast genomes and to develop useful genetic markers, we used a reference-assisted approach to assemble the complete chloroplast genome of omani lime (c. au ...201425398081
changes in anthocyanin production during domestication of citrus.mandarin (citrus reticulata), citron (citrus medica), and pummelo (citrus maxima) are important species of the genus citrus and parents of the interspecific hybrids that constitute the most familiar commercial varieties of citrus: sweet orange, sour orange, clementine, lemon, lime, and grapefruit. citron produces anthocyanins in its young leaves and flowers, as do species in genera closely related to citrus, but mandarins do not, and pummelo varieties that produce anthocyanins have not been repo ...201728196843
inhibition of metalloproteinase and proteasome activities in colon cancer cells by citrus peel extracts.citrus peels are consumed in the form of infusions, candy or wine, based on their well-documented nutritional and medicinal properties. this study sought to investigate the effect of some citrus peels' [grapefruit (citrus paradisii), orange (citrus sinensis) and shaddock (citrus maxima)] extracts on matrix metalloproteinase (mmp) and proteasome activities in primary human colonic tumor (caco-2) and the metastatic cell lines (lovo and lovo/adr) in a bid to explain the possible mechanism by which ...201526030791
sequencing of diverse mandarin, pummelo and orange genomes reveals complex history of admixture during citrus domestication.cultivated citrus are selections from, or hybrids of, wild progenitor species whose identities and contributions to citrus domestication remain controversial. here we sequence and compare citrus genomes--a high-quality reference haploid clementine genome and mandarin, pummelo, sweet-orange and sour-orange genomes--and show that cultivated types derive from two progenitor species. although cultivated pummelos represent selections from one progenitor species, citrus maxima, cultivated mandarins ar ...201424908277
characterization of the antioxidant properties of phenolic extracts from some citrus peels.this study sought to determine the distribution of free and bound phenolics in some nigerian citrus peels [orange (citrus sinensis), grapefruit (citrus paradisii) and shaddock (citrus maxima)] and characterize the antioxidant properties. the free phenolics were extracted with 80% acetone, while the bound phenolics were extracted from the alkaline and acid hydrolyzed residue with ethyl acetate. free phenolic extracts had significantly higher (p < 0.05) dpph* scavenging ability than the bound phen ...201224293692
anticholinesterase and antioxidative properties of water-extractable phytochemicals from some citrus peels.aqueous extracts from citrus peels are used in many rural communities in nigeria in treating various degenerative conditions, although the scientific basis for its use has not been well established. this study sought to investigate the anticholinesterase and antioxidant properties of aqueous extracts from some citrus peels [orange (citrus sinensis), grapefruit (citrus paradisii), and shaddock (citrus maxima)].201423907423
inhibition of acetylcholinesterase activity and fe2+-induced lipid peroxidation in rat brain in vitro by some citrus fruit juices.this study sought to characterize the effects of some citrus fruit juices (shaddock [citrus maxima], grapefruit [citrus paradisii], lemon [citrus limoni], orange [citrus sinensis], and tangerine [citrus reticulata]) on acetylcholinesterase activity in vitro. the total phenolic content, radical scavenging abilities, and inhibition of fe(2+)-induced malondialdehyde (mda) production in rats brain homogenate in vitro were also assessed. orange had significantly (p<.05) higher phenolic content than t ...201222400910
temperature thresholds and degree-day model for marmara gulosa (lepidoptera: gracillariidae).the developmental thresholds for marmara gulosa guillén & davis (lepidoptera: gracillariidae) were investigated in the laboratory by using 17, 21, 25, 29, and 33 degrees c. the lowest mortality occurred in cohorts exposed to 25 and 29 degrees c. other temperatures caused >10% mortality primarily in egg and first and second instar sap-feeding larvae. linear regression analysis approximated the lower developmental threshold at 12.2 degrees c. high mortality and slow developmental rate at 33 degree ...201121882694
tyramine pathways in citrus plant defense: glycoconjugates of tyramine and its n-methylated derivatives.glucosylated forms of tyramine and some of its n-methylated derivatives are here reported for the first time to occur in citrus genus plants. the compounds tyramine-o-β-d-glucoside, n-methyltyramine-o-β-d-glucoside, and n,n-dimethyltyramine-o-β-d-glucoside were detected in juice and leaves of sweet orange, bitter orange, bergamot, citron, lemon, mandarin, and pomelo. the compounds were identified by mass spectrometric analysis, enzymatic synthesis, and comparison with extracts of stapelia hirsut ...201728117581
a dark incubation period is important for agrobacterium-mediated transformation of mature internode explants of sweet orange, grapefruit, citron, and a citrange rootstock.citrus has an extended juvenile phase and trees can take 2-20 years to transition to the adult reproductive phase and produce fruit. for citrus variety development this substantially prolongs the time before adult traits, such as fruit yield and quality, can be evaluated. methods to transform tissue from mature citrus trees would shorten the evaluation period via the direct production of adult phase transgenic citrus trees.201223082165
comparative use of indel and ssr markers in deciphering the interspecific structure of cultivated citrus genetic diversity: a perspective for genetic association studies.genetic stratification associated with domestication history is a key parameter for estimating the pertinence of genetic association study within a gene pool. previous molecular and phenotypic studies have shown that most of the diversity of cultivated citrus results from recombination between three main species: c. medica (citron), c. reticulata (mandarin) and c. maxima (pummelo). however, the precise contribution of each of these basic species to the genomes of secondary cultivated species, su ...201222160318
limonoid content of sour orange varieties.modern citrus cultivars are thought to have arisen from three parents: the pummelo, the mandarin, and citron. taxological and genetic data support that sweet and sour oranges share a common parentage. however, as their name suggests, the organoleptic properties of the fruit from these two families is distinctly different. analysis of the limonoid content of sour orange varieties has been limited.201121681758
citrus tissue culture: stimulation of fruit explant cultures with orange juice.in vitro growth of explant (juice vesicle or albedo tissues) cultures from citron (citrus medica), lemon (c. limon), grapefruit (c. paradisi), sweet orange (c. sinensis), and mandarin (c. reticulata) fruits was stimulated by addition of orange juice (10% v/v optimum) to a basal medium containing murashige and skoog salts, 50 grams per liter sucrose, 100 milligrams per liter myo-inositol, 5 milligrams per liter thiamine.hcl, 2 milligrams per liter 2,4-dichlorophenoxyacetic acid and 0.5 milligrams ...197816660631
fast separation and sensitive quantitation of polymethoxylated flavonoids in the peels of citrus using uplc-q-tof-ms.a rapid, sensitive, and efficient ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (uplc-q-tof-ms) method has been developed to analyze polymethoxylated flavonoids (pmfs) in 14 citrus peels, including 7 citrus reticulata (c. reticulata) and 7 citrus sinensis (c. sinensis). in this study, fast separation can be achieved within 12 min and 42 pmfs have been identified including 33 flavones and 9 flavanones. most c. reticulata were shown to contain more ...201728260382
genome wide selection in citrus breeding.genome wide selection (gws) is essential for the genetic improvement of perennial species such as citrus because of its ability to increase gain per unit time and to enable the efficient selection of characteristics with low heritability. this study assessed gws efficiency in a population of citrus and compared it with selection based on phenotypic data. a total of 180 individual trees from a cross between pera sweet orange (citrus sinensis osbeck) and murcott tangor (citrus sinensis osbeck x ci ...201627813590
composition by lc-ms/ms of new carotenoid esters in mango and citrus.interest in the composition of carotenoid esters of fruits is growing because esterification may affect their bioavailability. thus, the aim was to provide a detailed identification of carotenoid esters in citrus and mango. orange cv. 'valencia' and cv. 'pera' presented 9 free carotenoids, 38 monoesters, and 60 diesters. violaxanthin and luteoxanthin derivatives were the major ones, followed by antheraxanthin, lutein, zeaxanthin, β-cryptoxanthin, and zeinoxanthin esters, many of them reported fo ...201627712060
coupling a branch enclosure with differential mobility spectrometry to isolate and measure plant volatiles in contained greenhouse settings.volatile organic compounds (vocs) are off-gassed from all living organisms and represent end products of metabolic pathways within the system. in agricultural systems, these vocs can provide important information on plant health and can ordinarily be measured non-invasively without harvesting tissue from the plants. previously we reported a portable gas chromatography/differential mobility spectrometry (gc/dms) system that could distinguish voc profiles of pathogen-infected citrus from healthy t ...201626695246
detection of mandarin in orange juice by single-nucleotide polymorphism qpcr assay.a dual-probe real time pcr (qpcr) dna-based analysis was devised for the identification of mandarin in orange juice. a single nucleotide polymorphism at the trnl-trnf intergenic region of the chloroplast chromosome was confirmed in nine orange (citrus sinensis) and thirteen commercial varieties of mandarin, including citrus reticulata and citrus unshiu species and a mandarin × tangelo hybrid. two short minor-groove binding fluorescent probes targeting the polymorphic sequence were used in the du ...201424128588
chemical composition and anti-inflammatory effects of essential oil from hallabong flower.a number of essential oils derived from plants are claimed to have several medicinal functions, including anti-cancer and anti-inflammation effects. however, the chemical composition and biological activities of flower-derived components have not been sufficiently characterized. therefore, we investigated the composition of essential oils from hallabong flower [(citrus unshiu marcov × citrus sinensis osbeck) × citrus reticulata blanco] and their anti-inflammatory effects. hydro-distilled essenti ...201327366141
first record of anastrepha serpentina (wiedemann) (diptera: tephritidae) in citrus in brazil.anastrepha serpentina (wiedemann) is recorded for the first time in citrus (rutaceae) in brazil. specimens were obtained from sweet orange (citrus sinensis) sampled in the municipalities of belém and capitão poço, and from mandarin orange (citrus reticulata) from tomé-açu, state of pará, brazil.201323939279
somatic hybrid plants from sexually incompatible woody species: citrus reticulata and citropsis gilletiana.allotetraploid intergeneric somatic hybrid plants between citrus reticulata blanco cv. cleopatra mandarin and citropsis gilletiana swing. & m. kell. (common name gillet's cherry orange) were regenerated following protoplast fusion. cleopatra protoplasts were isolated from an ovule-derived embryogenic suspension culture and fused chemically with leaf-derived protoplasts of citropsis gilletiana. cleopatra mandarin and somatic hybrid plants were regenerated via somatic embryogenesis. hybrid plant i ...199024232779
citrus leprosis virus n: a new dichorhavirus causing citrus leprosis disease.citrus leprosis (cl) is a viral disease endemic to the western hemisphere that produces local-necrotic and -chlorotic lesions on leaves, branches and fruits and causes serious yield reduction in citrus orchards. samples of sweet orange (citrus × sinensis) trees showing cl symptoms were collected during a survey in non-commercial citrus areas in the southeast region of brazil in 2013-2016. transmission electron microscopy analyses of foliar lesions confirmed the presence of rod-like viral particl ...201728398876
cytological and molecular characterization of carotenoid accumulation in normal and high-lycopene mutant oranges.ripe cara cara sweet orange contains 25 times as much carotenoids in flesh as newhall sweet orange, due to high accumulation of carotenes, mainly phytoene, lycopene and phytofluene. only yellow globular chromoplasts were observed in newhall flesh. distinct yellow globular and red elongated crystalline chromoplasts were found in cara cara but only one type of chromoplast was present in each cell. the red crystalline chromoplasts contained lycopene as a dominant carotenoid and were associated with ...201728396598
kinetic and isotherm studies of bisphenol a adsorption onto orange albedo(citrus sinensis): sorption mechanisms based on the main albedo components vitamin c, flavones glycosides and carotenoids.orange albedo and its adsorption capacity towards bisphenol a (bpa) were studied. adsorption experiments were conducted in batch mode at 25-55°c. scanning electron microscopy (sem), energy dispersive x-ray spectroscopy (edx), x-ray diffraction (xrd), brunauer-emmett-teller (bet) and fourier transform infrared (ftir) spectroscopy were used to characterise the biosorbent. the effects of various parameters including adsorption time, equilibrium ph, adsorbent dosage and initial adsorbate concentrati ...201728394738
comparison of two dna extraction protocols from leave samples of cotinus coggygria, citrus sinensis and genus juglans.high quality dna is essential for molecular research. secondary metabolites can affect the quantity and quality dna. in current research two dna isolation methods including ctab and delaporta (protocols 1 &amp; 2 respectively) were applied in three leave samples from cotinus coggygria, citrus sinensis and genus juglans that their leaves are rich of secondary metabolites. we successfully isolated dna from c. coggygria, c. sinensis and genus juglans using the two protocols described above. good qu ...201728364796
one target, two mechanisms: the impact of candidatus liberibacter asiaticus and its vector, diaphorina citri on citrus leaf pigments.huanglongbing is currently the largest threat to global citrus production. we examined the effect of hlb pathogen, candidatus liberibacter asiaticus-infection and/or its vector, diaphorina citri-infestation on valencia sweet orange leaf pigments using hplc, followed by gene expression analysis for 46 involved genes in carotenoids- and chlorophylls-biosynthesis pathways. both clas and d. citri alter the total citrus leaf pigments balance with a greater impact by clas. while zeaxanthin was accumul ...201728358623
root adaptive responses to aluminum-treatment revealed by rna-seq in two citrus species with different aluminum-tolerance.seedlings of aluminum (al)-tolerant citrus sinensis and al-intolerant citrus grandis were fertigated daily with nutrient solution containing 0 and 1.0 mm alcl3●6h2o for 18 weeks. the al-induced decreases of biomass and root total soluble proteins only occurred in c. grandis, demonstrating that c. sinensis had higher al-tolerance than c. grandis. under al-treatment, c. sinensis roots secreted more citrate and malate than c. grandis ones; less al was accumulated in c. sinenis than in c. grandis le ...201728337215
investigation on key molecules of huanglongbing (hlb)-induced orange juice off-flavor.orange fruits from huanglongbing (hlb)-infected trees do not fully mature and show a severe off-flavor described as bitter-harsh, metallic, and less juicy and fruity. the investigation of juice from hlb-infected (hlboj) and healthy control oranges (coj) by gas chromatography-mass spectrometry showed higher concentrations of fruity esters, such as ethyl butyrate and ethyl 2-methylbutyrate, and soapy-waxy alkanals, such as octanal and decanal, in the coj, whereas the hlboj showed higher concentrat ...201728285516
structural characterization of early michaelis complexes in the reaction catalyzed by (+)-limonene synthase from citrus sinensis using fluorinated substrate analogues.the stereochemical course of monoterpene synthase reactions is thought to be determined early in the reaction sequence by selective binding of distinct conformations of the geranyl diphosphate (gpp) substrate. we explore here formation of early michaelis complexes of the (+)-limonene synthase [(+)-ls] from citrus sinensis using monofluorinated substrate analogues 2-fluoro-gpp (fgpp) and 2-fluoroneryl diphosphate (fnpp). both are competitive inhibitors for (+)-ls with ki values of 2.4 ± 0.5 and 3 ...201728272876
functional and structural characterization of a (+)-limonene synthase from citrus sinensis.terpenes make up the largest and most diverse class of natural compounds and have important commercial and medical applications. limonene is a cyclic monoterpene (c10) present in nature as two enantiomers, (+) and (-), which are produced by different enzymes. the mechanism of production of the (-)-enantiomer has been studied in great detail, but to understand how enantiomeric selectivity is achieved in this class of enzymes, it is important to develop a thorough biochemical description of enzyme ...201728272875
effects of low ph on photosynthesis, related physiological parameters, and nutrient profiles of citrus.seedlings of "xuegan" (citrus sinensis) and "sour pummelo" (citrus grandis) were irrigated daily with a nutrient solution at a ph of 2.5, 3, 4, 5, or 6 for 9 months. thereafter, the following responses were investigated: seedling growth; root, stem, and leaf concentrations of nutrient elements; leaf gas exchange, pigment concentration, ribulose-1,5-bisphosphate carboxylase/oxygenase activity and chlorophyll a fluorescence; relative water content, total soluble protein level, h2o2 production and ...201728270819
effects of high toxic boron concentration on protein profiles in roots of two citrus species differing in boron-tolerance revealed by a 2-de based ms approach.citrus are sensitive to boron (b)-toxicity. in china, b-toxicity occurs in some citrus orchards. so far, limited data are available on b-toxicity-responsive proteins in higher plants. thirteen-week-old seedlings of "sour pummelo" (citrus grandis) and "xuegan" (citrus sinensis) was fertilized every other day until dripping with nutrient solution containing 10 μm (control) or 400 μm (b-toxicity) h3bo3 for 15 weeks. the typical b-toxic symptom only occurred in 400 μm b-treated c. grandis leaves, an ...201728261239
accurate measurement of transgene copy number in crop plants using droplet digital pcr.genetic transformation is a powerful means for the improvement of crop plants, but requires labor- and resource-intensive methods. an efficient method for identifying single-copy transgene insertion events from a population of independent transgenic lines is desirable. currently, transgene copy number is estimated by either southern blot hybridization analyses or quantitative polymerase chain reaction (qpcr) experiments. southern hybridization is a convincing and reliable method, but it also is ...201728231382
a systematic review of the anxiolytic-like effects of essential oils in animal models.the clinical efficacy of standardized essential oils (such as lavender officinalis), in treating anxiety disorders strongly suggests that these natural products are an important candidate source for new anxiolytic drugs. a systematic review of essential oils, their bioactive constituents, and anxiolytic-like activity is conducted. the essential oil with the best profile is lavendula angustifolia, which has already been tested in controlled clinical trials with positive results. citrus aurantium ...201526473822
experimental evidence and in silico identification of tryptophan decarboxylase in citrus genus.plant tryptophan decarboxylase (tdc) converts tryptophan into tryptamine, precursor of indolealkylamine alkaloids. the recent finding of tryptamine metabolites in citrus plants leads to hypothesize the existence of tdc activity in this genus. here, we report for the first time that, in citrus x limon seedlings, deuterium labeled tryptophan is decarboxylated into tryptamine, from which successively deuterated n,n,n-trimethyltryptamine is formed. these results give an evidence of the occurrence of ...201728208655
tetraploid rangpur lime rootstock increases drought tolerance via enhanced constitutive root abscisic acid production.whole-genome duplication, or polyploidy, is common in many plant species and often leads to better adaptation to adverse environmental condition. however, little is known about the physiological and molecular determinants underlying adaptation. we examined the drought tolerance in diploid (2x) and autotetraploid (4x) clones of rangpur lime (citrus limonia) rootstocks grafted with 2x valencia delta sweet orange (citrus sinensis) scions, named v/2xrl and v/4xrl, respectively. physiological experim ...201323050986
changes in volatile and non-volatile flavor chemicals of "valencia" orange juice over the harvest seasons.florida "valencia" oranges have a wide harvest window, covering four months after first reaching the commercial maturity. however, the influence of harvest time on juice flavor chemicals is not well documented, with the exception of sugars and acids. therefore, we investigated the major flavor chemicals, volatile (aroma), non-volatile (taste) and mouth feel attributes, in the two harvest seasons (march to june in 2007 and february to may in 2012). bitter limonoid compounds, limonin and nomilin, ...201628231099
two previously unknown phytophthora species associated with brown rot of pomelo (citrus grandis) fruits in vietnam.two distinct phytophthora taxa were found to be associated with brown rot of pomelo (citrus grandis), a new disease of this ancestral citrus species, in the vinh long province, mekong river delta area, southern vietnam. on the basis of morphological characters and using the its1-5.8s-its2 region of the rdna and the cytochrome oxidase subunit 1 (coi) as barcode genes, one of the two taxa was provisionally named as phytophthora sp. prodigiosa, being closely related to but distinct from p. insolita ...201728208159
natural compounds from mexican medicinal plants as potential drug leads for anti-tuberculosis drugs.in mexican traditional medicine 187 plant species are used in the treatment of respiratory conditions that may be associated with tuberculosis. in this contribution, we review the ethnobotany, chemistry and pharmacology of 63 species whose extracts have been assayed for antimycobacterial activity in vitro. among these, the most potent is aristolochia brevipes (mic= 12.5 µg/ml), followed by aristolochia taliscana, citrus sinensis, chrysactinia mexicana, persea americana, and olea europaea (mic<64 ...201728198919
encapsulation of polymethoxyflavones in citrus oil emulsion-based delivery systems.the purpose of this work was to elucidate the effects of citrus oil type on polymethoxyflavone (pmf) solubility and on the physicochemical properties of pmf-loaded emulsion-based delivery systems. citrus oils were extracted from mandarin, orange, sweet orange, and bergamot. the major constituents were determined by gc/ms: sweet orange oil (97.4% d-limonene); mandarin oil (72.4% d-limonene); orange oil (67.2% d-limonene); and bergamot oil (34.6% linalyl acetate and 25.3% d-limonene). pmf-loaded e ...201728182421
depletion of abscisic acid levels in roots of flooded carrizo citrange (poncirus trifoliata l. raf. × citrus sinensis l. osb.) plants is a stress-specific response associated to the differential expression of pyr/pyl/rcar receptors.soil flooding reduces root abscisic acid (aba) levels in citrus, conversely to what happens under drought. despite this reduction, microarray analyses suggested the existence of a residual aba signaling in roots of flooded carrizo citrange seedlings. the comparison of aba metabolism and signaling in roots of flooded and water stressed plants of carrizo citrange revealed that the hormone depletion was linked to the upregulation of csaog, involved in aba glycosyl ester (abage) synthesis, and to a ...201728160166
effect of pasteurization on flavonoids and carotenoids in citrus sinensis (l.) osbeck cv. 'cara cara' and 'bahia' juices.orange juice is considered an excellent dietary source of several bioactive compounds with beneficial properties for human health. citrus sinensis osbeck cv. 'cara cara' is a bud mutation originated from 'washington' navel orange, also known as 'bahia' navel orange. the ascorbic acid, flavonoid, and carotenoid contents in pasteurized and nonpasteurized bahia and cara cara juices using two lc-ms/ms platforms were investigated. higher ascorbic acid content was observed in bahia compared to cara ca ...201728146357
leaf-disc grafting for the transmission of candidatus liberibacter asiaticus in citrus (citrus sinensis; rutaceae) seedlings.the search for resistance/tolerance to the devastating citrus huanglongbing disease (syn. hlb or citrus greening) is generating an increasing number of new plants of diverse genetic makeup. as the increasing number of new plants require more space, resources, and time, the need for faster and more efficient hlb screening tests becomes crucial.201728090406
physiologic, anatomic, and gene expression changes in citrus sunki, poncirus trifoliata, and their hybrids after 'candidatus liberibacter asiaticus' infection.huanglongbing (hlb) is a destructive disease of citrus caused by phloem-limited bacteria, namely 'candidatus liberibacter asiaticus' (las), 'candidatus liberibacter africanus', and 'candidatus liberibacter americanus'. although there are no known hlb-resistant citrus species, studies have reported poncirus trifoliata as being more tolerant. assuming that callose deposition in the phloem of infected plants can inhibit translocation of photosynthetic products and cause starch accumulation, we comp ...201728068188
microrna annotation of plant genomes - do it right or not at all.micrornas are non-coding regulators of gene expression and key factors in development, disease, and targets for bioengineering. consequently, micrornas have become essential elements of already burgeoning draft plant genome descriptions where their annotation is often particularly poor, contributing unduly to the corruption of public databases. using the citrus sinensis as an example, we highlight and review common failings of mirnaome annotations. understanding and exploiting the role of mirnas ...201728054378
assessing a traceability technique in fresh oranges (citrus sinensis l. osbeck) with an hs-spme-gc-ms method. towards a volatile characterisation of organic oranges.a targeted approach using hs-spme-gc-ms was performed to compare flavour compounds of 'navelina' and 'salustiana' orange cultivars from organic and conventional management systems. both varieties of conventional oranges showed higher content of ester compounds. on the other hand, higher content of some compounds related with the geranyl-diphosphate pathway (neryl and geranyl acetates) and some terpenoids were found in the organic samples. furthermore, the partial least square discriminant analys ...201727979182
analysis of whole chloroplast genomes from the genera of the clauseneae, the curry tribe (rutaceae, citrus family).the clauseneae (aurantioideae, rutaceae) is a tribe in the citrus family that, although economically important as it contains the culinary and medicinally-useful curry tree (bergera koenigii), has been relatively understudied. due to the recent significant taxonomic changes made to this tribe, a closer inspection of the genetic relationships among its genera has been warranted. whole genome skimming was used to generate chloroplast genomes from six species, representing each of the four genera ( ...201627965082
genome-wide identification and functional analysis of s-rnase involved in the self-incompatibility of citrus.s-rnase-based self-incompatibility is found in solanaceae, rosaceae, and scrophulariaceae, and is the most widespread mechanism that prevents self-fertilization in plants. although 'shatian' pummelo (citrus grandis), a traditional cultivated variety, possesses the self-incompatible trait, the role of s-rnases in the self-incompatibility of 'shatian' pummelo is poorly understood. to identify genes associated with self-incompatibility in citrus, we identified 16 genes encoding homologs of ribonucl ...201727933381
hybrid origins of citrus varieties inferred from dna marker analysis of nuclear and organelle genomes.most indigenous citrus varieties are assumed to be natural hybrids, but their parentage has so far been determined in only a few cases because of their wide genetic diversity and the low transferability of dna markers. here we infer the parentage of indigenous citrus varieties using simple sequence repeat and indel markers developed from various citrus genome sequence resources. parentage tests with 122 known hybrids using the selected dna markers certify their transferability among those hybrid ...201627902727
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