| inotropic effect of citrus sinensis (l.) osbeck leaf extracts on the guinea pig atrium. | the objective of the present investigation was to determine the contractile effect of crude and acetone leaf extracts of citrus sinensis (l.) osb. on mammalian myocardium. crude leaf extracts have been used in folk medicine to treat neurological disorders. some flavonoids isolated from this plant presented a positive inotropic effect on myocardium. this motivated us to test the extracts on the atria of guinea pigs of both sexes (300-500 g) and surprisingly we observed inotropic depression instea ... | 2005 | 15665997 |
| adult diet and male-female contact effects on female reproductive potential in mexican fruit fly (anastrepha ludens loew) (diptera tephritidae). | wild strains of fruit flies (diptera: tephritidae) placed into laboratory rearing conditions are subjected to selection pressures caused by the diet, cages, density of flies, and other factors. selection that changes mating behavior of the strain may result in less effective males released in sterile insect programs. tests were performed to examine the effects of protein in diet and adult interactions on egg production and mating during sexual maturation of the mexican fruit fly (anastrepha lude ... | 2003 | 14994799 |
| the role of carotenoid cleavage dioxygenases in the regulation of carotenoid profiles during maturation in citrus fruit. | to investigate the relationship between a carotenoid profile and gene expression for carotenoid cleavage dioxygenases, three citrus varieties that exhibit different 9-cis-violaxanthin levels in their juice sacs, satsuma mandarin (citrus unshiu marc.; a variety accumulating a low level of 9-cis-violaxanthin), valencia orange (citrus sinensis osbeck; variety accumulating a high level of 9-cis-violaxanthin), and lisbon lemon (citrus limon burm.f.; a variety accumulating an undetectable level of 9-c ... | 2006 | 16714310 |
| potent inhibition by star fruit of human cytochrome p450 3a (cyp3a) activity. | there has been very limited information on the capacities of tropical fruits to inhibit human cytochrome p450 3a (cyp3a) activity. thus, the inhibitory effects of tropical fruits on midazolam 1'-hydroxylase activity of cyp3a in human liver microsomes were evaluated. eight tropical fruits such as common papaw, dragon fruit, kiwi fruit, mango, passion fruit, pomegranate, rambutan, and star fruit were tested. we also examined the inhibition of cyp3a activity by grapefruit (white) and valencia orang ... | 2004 | 15155547 |
| nitrate improves growth in salt-stressed citrus seedlings through effects on photosynthetic activity and chloride accumulation. | we analyzed the effects of nitrate availability on growth of navelina (citrus sinensis (l.) osbeck) scions grafted on three citrus rootstocks differing in salt tolerance: carrizo citrange (citrus sinensis (l.) osbeck x poncirus trifoliata (l.) raf.), citrus macrophylla wester and cleopatra mandarin (citrus reshni hort. ex tanaka). salt stress reduced total plant biomass by 27-38%, whereas potassium nitrate supplementation partially counteracted this effect by increasing dry matter and new leaf a ... | 2004 | 15234900 |
| a rapid liquid chromatography electrospray ionization mass spectrometry(n) method for evaluation of synephrine in citrus aurantium l. samples. | immature bitter orange fruit and its extracts have been introduced into the market as an alternative to ephedra in weight loss products. however, the safety of the immature bitter orange fruit and its extracts is a debated argument due to the presence of synephrine, a constituent known as a sympathomimetic agent. in this paper, we describe the development of a new, rapid, and simple liquid chromatography-electrospray ionization-tandem mass spectrometry method devoted to the quantitative determin ... | 2005 | 16366666 |
| quantitative real-time pcr for detection and identification of candidatus liberibacter species associated with citrus huanglongbing. | citrus huanglongbing (hlb, ex greening) is one of the most serious diseases of citrus. different forms of the disease are caused by different candidatus liberobacter species, candidatus liberibacter asiaticus (las), ca. l. africanus (laf) and ca. l. americanus (lam). the pathogen is transmitted by psyllid insects and by budding with contaminated plant materials. the vector psyllid diaphorina citri can transmit both las and lam. establishment of this vector into florida, reports of lam and las in ... | 2006 | 16414133 |
| comparative toxicity effect of bush tea leaves (hyptis suaveolens) and orange peel (citrus sinensis) oil extract on larvae of the yellow fever mosquito aedes aegypti. | the ethanolic extracts of the orange peel (citrus sinensis) and bush tea leaves (hyptis suaveolens) were compared for their toxicity effect on the larvae of the yellow fever mosquito aedes aegypti collected from disused tyres beside college of natural sciences building university of agriculture, abeokuta, nigeria. eight graded concentrations, 0.9ppm, 0.8ppm, 0.7ppm, 0.6ppm, 0.5ppm, 0.4ppm, 0.3ppm and 0.2ppm of both plant extracts were tested on the larvae. the mean lethal dose ld10, was 0.15 ppm ... | 2005 | 16941944 |
| toward validation of process criteria for high-pressure processing of orange juice with predictive models. | mathematical models were developed to predict time to inactivation (tti) by high-pressure processing of salmonella in australian valencia orange juice (ph 4.3) and navel orange juice (ph 3.7) as a function of pressure magnitude (300 to 600 mpa) and inoculum level (3 to 7 log cfu/ml). for each model, the tti was found to increase with increasing inoculum level and decrease with increasing pressure magnitude. the u.s. food and drug administration juice hazard analysis and critical control point re ... | 2005 | 15895726 |
| inhibition of aflatoxin b production of aspergillus flavus, isolated from soybean seeds by certain natural plant products. | the inhibitory effect of cowdung fumes, captan, leaf powder of withania somnifera, hyptis suaveolens, eucalyptus citriodora, peel powder of citrus sinensis, citrus medica and punica granatum, neem cake and pongamia cake and spore suspension of trichoderma harzianum and aspergillus niger on aflatoxin b(1) production by toxigenic strain of aspergillus flavus isolated from soybean seeds was investigated. | 2006 | 17032218 |
| varietal and interspecific influence on micronutrient contents in citrus from the mediterranean area. | to specify the genotypic variation of mediterranean citrus juices, the contents of carotenoids, flavonoids, and vitamin c were determined by high-performance liquid chromatography. a selection of orange varieties and mandarin species from the mediterranean area (citrus sinensis, citrus deliciosa ten, and citrus clementina hort. ex tan) was evaluated using carotenoid profiles and flavanones contents. among the eight varieties of orange (salustiana, hamlin, shamouti, pera, valencia, maltaise, sang ... | 2005 | 15769147 |
| protoplast transformation and regeneration of transgenic valencia sweet orange plants containing a juice quality-related pectin methylesterase gene. | valencia orange [citrus sinensis (l.) osbeck] is the leading commercial citrus species in the world for processed juice products; however, the presence of thermostable pectin methylesterase (tspme) reduces its juice quality. a long-term strategy of this work is to eliminate or greatly reduce tspme activity in valencia orange. previous work resulted in the isolation of a putative tspme gene, cspme4, associated with a thermostable protein fraction of valencia orange juice. to begin research design ... | 2005 | 15875190 |
| [chemical composition and bioactive compounds of flour of orange (citrus sinensis), tangerine (citrus reticulata) and grapefruit (citrus paradisi) peels cultivated in venezuela]. | the purpose of this research was to evaluate the chemical composition and some bioactive compounds in the peel's flour of some of the most consumed citrus fruits cultivated in venezuela. chemical composition as well as some trace elements, ascorbic acid, carotenoids dietary fiber, total polyphenols and their antiradical efficiency, using the 2,2-diphenyl-1-picrylhidracyl (dpph) were assessed in the dried peels of orange (citrus sinensis), tangerine (citrus reticulata) and white grapefruit (citru ... | 2005 | 16454058 |
| calibration of sap flow estimated by the compensation heat pulse method in olive, plum and orange trees: relationships with xylem anatomy. | the compensation heat pulse method is widely used to estimate sap flow in conducting organs of woody plants. being an invasive technique, calibration is crucial to derive correction factors for accurately estimating the sap flow value from the measured heat pulse velocity. we compared the results of excision and perfusion calibration experiments made with mature olive (olea europaea l. 'manzanilla de sevilla'), plum (prunus domestica l. 'songal') and orange (citrus sinensis (l.) osbeck. 'cadener ... | 2006 | 16510387 |
| fatty acid methyl ester profiles for characterization of glomalean fungi and their endomycorrhizae. | arbuscule-forming fungi in the order glomales form obligate endomycorrhizal associations with plants that make them difficult to quantify, and taxonomy of the group is only beginning to be objectively understood. fatty acid methyl ester (fame) profiles were analyzed to assess the diversity and quantity of fatty acids in 53 isolates of 24 glomalean species. spores and endomycorrhizal roots of sudan grass (sorghum sudanense) and the citrus rootstock carrizo citrange (poncirus trifoliata x citrus s ... | 1995 | 16534923 |
| antioxidant activity of the flavonoid hesperidin in chemical and biological systems. | the antioxidant hesperidin, a major flavonoid in sweet orange and lemon, was evaluated using chemical and biological systems. the chemical assay evaluates the hesperidin capacity to sequester 1,1-diphenyl-2-picrylhydrazyl (dpph*). biological studies were done using the eukaryotic cells of superoxide-dismutase proficient and deficient strains of saccharomyces cerevisiae treated with hesperidin and the stressing agents hydrogen peroxide or paraquat (methylviologen; 1,1'-dimethyl-4,4'-bipyridinium ... | 2005 | 15941311 |
| modulation of p-glycoprotein activity by acridones and coumarins from citrus sinensis. | bioguided fractionation of the roots of citrus sinensis (rutaceae) led to the isolation and identification of five coumarins, namely, clausarin, suberosin, poncitrin, xanthyletin and thamnosmonin, seven acridones, namely, acrimarine b, 2-methoxycitpressine i, citpressine i, buntanine, acrimarine e, honyumine and acrimarine c, and one terpenoid, namely, limonin. among these compounds, clausarin, 2-methoxycitpressine i and acrimarine e inhibited p-glycoprotein-mediated drug efflux in k562/r7 human ... | 2007 | 17236173 |
| efficacy of bacillus thuringiensis against phyllocnistis citrella (lepidoptera: phyllocnistidae). | the purpose of this work was to analyze the efficacy of bacillus thuringiensis berliner on the control of phyllocnistis citrella stainton (lepidoptera: phyllocnistidae) in laboratory and field trials. in the laboratory, four b. thuringiensis were used: dipel (commercial formulation) tested at the concentrations of 25 x 10(6) and 25 x 10(8) spores/ml and the isolates k, 6, and 15 (collections of the university of azores) at the concentration of 25 x 10(6) spores/ ml. a surfactant solution of nono ... | 2005 | 16539108 |
| the occurrence of abscisic acid and abscisyl-beta-d-glucopyranoside in developing and mature citrus fruit as determined by enzyme immunoassay. | the contents of (+)-cis-abscisic acid (aba) and alkaline-hydrolyzable aba-conjugate(s) were analyzed by means of enzyme immunoassay in partially purified extracts of developing and mature sweet orange fruit (citrus sinensis [l.] osbeck cv washington navel). a relatively small increase in aba was observed in the fruit exocarp during the natural color transition from green to orange. at the same time, the aba-conjugate level increased approximately 12-fold in this tissue. the contents of aba and a ... | 1986 | 16665032 |
| [control effects of mikania micrantha alcohol extract on citrus red mite panonychus citri]. | this study showed that spraying 0.2 and 0.4 g x l(-1) mikania micrantha alcohol extracts on panonychus citri could significantly decrease the survival rates of p. citri eggs, larvae and nymphs, and 0.1 g x l(-1) m. micrantha alcohol extract could also significantly decrease the survival rates of larvae and nymphs. in a no-choice test, both the fecundity and the longevity of female p. citri fed on the leaves treated with all three test concentrations 0.1, 0.2 and 0.4 g x l(-1) of m. micrantha alc ... | 2005 | 16011182 |
| carbon cost of the fungal symbiont relative to net leaf p accumulation in a split-root va mycorrhizal symbiosis. | translocation of (14)c-photosynthates to mycorrhizal (+ +), half mycorrhizal (0+), and nonmycorrhizal (00) split-root systems was compared to p accumulation in leaves of the host plant. carrizo citrange seedlings (poncirus trifoliata [l.] raf. x citrus sinensis [l.] osbeck) were inoculated with the vesicular-arbuscular mycorrhizal fungus glomus intraradices schenck and smith. plants were exposed to (14) co(2) for 10 minutes and ambient air for 2 hours. three to 4% of recently labeled photosyntha ... | 1988 | 16665934 |
| evaluation of endophytic colonization of citrus sinensis and catharanthus roseus seedlings by endophytic bacteria. | over the last few years, the endophytic bacterial community associated with citrus has been studied as an important component interacting with xylella fastidiosa, the causal agent of citrus variegated chlorosis (cvc). this bacterium may also colonize some model plants, such as catharanthus roseus and nicotiana clevelandii. in the present study, we compared the endophytic colonization of citrus sinensis and catharanthus roseus using the endophytic bacteria klebsiella pneumoniae. we chose an appro ... | 2007 | 17342049 |
| common subtropical and tropical nonpollen food sources of the boll weevil (coleoptera: curculionidae). | it is known that substantial boll weevil, anthonomus grandis grandis boheman, individuals can survive mild subtropical winters in some habitats, such as citrus orchards. our study shows that endocarp of the fruit from prickly pear cactus, opuntia engelmannii salm-dyck ex. engel.; orange, citrus sinensis l. osbeck.; and grapefruit, citrus paradisi macfad., can sustain newly emerged adult boll weevils for >5 mo, which is the duration of the cotton-free season in the subtropical lower rio grande va ... | 2007 | 17349122 |
| [creation and hybrid identification of the sexual progenies using citrus somatic hybrid as pollen parent]. | 110 plantlets were obtained from the cross between citrus allotetraploid somatic hybrid [hamlin sweet orange (citrus sinensis osback) + rough lemon (c. jambhiri luss)] (as the pollen parent) and mono-embryonic diploid type iben no.4 [huanongbendizao tangerine (c. reticulata blanco) x ichang papeda (c. ichangensis swingle)] by the means of young embryo culture in vitro, and there were 93 triploids and 17 diploids through chromosome counting and the ploidy analyser identification. rapd analyses sh ... | 2005 | 16044915 |
| water deficit and associated changes in some photosynthetic parameters in leaves of ;valencia' orange (citrus sinensis [l.] osbeck). | photosynthetic co(2) assimilation, transpiration, ribulose-1,5-bisphosphate carboxylase (rubpcase), and soluble protein were reduced in leaves of water-deficit (stress) ;valencia' orange (citrus sinensis [l.] osbeck). maximum photosynthetic co(2) assimilation and transpiration, which occurred before midday for both control and stressed plants, was 58 and 40%, respectively, for the stress (-2.0 megapascals leaf water potential) as compared to the control (-0.6 megapascals leaf water potential). a ... | 1988 | 16666311 |
| transcription analysis of pils and xpsel genes from xylella fastidiosa. | xylella fastidiosa is a xylem-limited phytopathogen responsible for diseases in several plants such as citrus and coffee. analysis of the bacterial genome revealed some putative pathogenicity-related genes that could help to elucidate the molecular mechanisms of plant-pathogen interactions. in the present work, the transcription of three genes of the bacterium, grown in defined and rich media and also in media containing host plant extracts (sweet orange, 'ponkan' and coffee) was analyzed by rt- ... | 2005 | 15803391 |
| effect of certain bioactive plant extracts on clinical isolates of beta-lactamase producing methicillin resistant staphylococcus aureus. | ethanolic extracts and some fractions from 10 indian medicinal plants, known for antibacterial activity, were investigated for their ability to inhibit clinical isolates of beta-lactamase producing methicillin-resistant staphylococcus aureus (mrsa) and methicillin-sensitive s. aureus (mssa). synergistic interaction of plant extracts with certain antibiotics was also evaluated. the mrsa test strains were found to be multi-drug resistant and also exhibited high level of resistance to common beta-l ... | 2005 | 15812867 |
| identification of endogenous gibberellins in navel orange shoots. | eight gibberellins (gas) were identified from vegetative shoots of navel orange trees (citrus sinensis l. osbeck cv washington) after sequential purification by reverse-phase c(18) high performance liquid chromatography, nucleosil 5n(ch(3))(2) high performance liquid chromatography, and capillary gas chromatography-mass spectrometry. ga(1), ga(17), ga(19), ga(20), ga(29), and iso-ga(3) were identified based on the full scan mass spectra and kovats retention indices. ga(8) was tentatively identif ... | 1988 | 16666361 |
| effect of temperature on development and survival of the parasitoid pnigalio pectinicornis (hymenoptera: eulophidae) reared on phyllocnistis citrella (lepidoptera: gracillariidae). | laboratory studies were conducted to assess the effect of temperature on the development and survival of the indigenous parasitoid pnigalio pectinicornis l. on the citrus leaf miner phyllocnistis citrella stainton as host, fed on leaves of citrus sinensis l. osbck cultivar washington navel and citrus reticulata blanco cultivar clementine. experiments were conducted at five constant temperatures ranging from 15 to 32.5 degrees c, with 60 +/- 10% rh and a photoperiod of 14:10 (l:d) h. the relation ... | 2007 | 17540056 |
| soil water repellency induced by long-term irrigation with treated sewage effluent. | this study describes soil water repellency developed under prolonged irrigation with treated sewage effluent in a semiarid environment. soil surface layer (0-5 cm) and soil profile (0-50 cm) transects were sampled at a high resolution at the close of the irrigation season and rainy winter season. samples from 0- to 5-cm transects were subdivided into 1-cm slices to obtain fine scale resolution of repellency and organic matter distribution. extreme to severe soil water repellency in the 0- to 5-c ... | 2005 | 16151242 |
| establishment of a genetically marked insect-derived symbiont in multiple host plants. | alcaligenes xylosoxidans subsp. denitrificans, originally isolated from the cibarial region of the foregut of the glassy-winged sharpshooter (homalodisca coagulata), was transformed using the himar1 transposition system to express egfp. seedlings of six potential host plants were inoculated with transformed bacteria and 2 weeks later samples were taken 5 cm away and analyzed by quantitative real-time pcr using primers designed to amplify the gene insert. the largest colony of 3,591,427 cells/2 c ... | 2005 | 15723145 |
| biological activity of carotenoids in red paprika, valencia orange and golden delicious apple. | carotenoid fractions were extracted from red paprika, valencia orange peel and the peel of golden delicious apple. thus, hypophasic carotenoids of paprika (pm1), orange (pm3) and apple (pm4), and epiphasic extractions of paprika (pm2) and apple (pm5) were obtained by extraction, saponification and partition between meoh-h(2)o (9:1) (hypophasic) and hexane (epiphasic). a high content of capsanthin was quantified in hypophasic carotenoids (pm1) from red spice paprika, whereas the hypophasic fracti ... | 2005 | 16177974 |
| effects of ozone and sulfur dioxide on phyllosphere fungi from three tree species. | short-term effects of ozone (o(3)) on phyllosphere fungi were studied by examining fungal populations from leaves of giant sequoia (sequoiadendron giganteum (lindl.) buchholz) and california black oak (quercus kelloggii newb.). chronic effects of both o(3) and sulfur dioxide (so(2)) were studied by isolating fungi from leaves of mature valencia orange (citrus sinensis l.) trees. in this chronic-exposure experiment, mature orange trees were fumigated in open-top chambers at the university of cali ... | 1989 | 16347849 |
| efficient production of transgenic citrus plants using isopentenyl transferase positive selection and removal of the marker gene by site-specific recombination. | the presence of marker genes conferring antibiotic resistance in transgenic plants represents a serious obstacle for their public acceptance and future commercialization. in citrus, selection using the selectable marker gene nptii, that confers resistance to the antibiotic kanamycin, is in general very effective. an attractive alternative is offered by the mat system (multi-auto-transformation), which combines the ipt gene for positive selection with the recombinase system r/rs for removal of ma ... | 2007 | 16927091 |
| biocontrol of aflatoxins b1, b2, g1, g2, and fumonisin b1 with 6,7-dimethoxycoumarin, a phytoalexin from citrus sinensis. | phytoalexins (stress-induced compounds) from citrus sinensis cultivar valencia were screened for antifungal and antimycotoxic activity against a test organism (cladosporium cladosporoides) and mycotoxin-producing fungi fusarium verticillioides and aspergillus parasiticus. the active compound, a member of the coumarin family of compounds, has antifungal and antimycotoxic activities and was chemically identified. high-performance liquid chromatography results indicated that valencia oranges contai ... | 2006 | 16995528 |
| polymethoxylated flavones induce ca(2+)-mediated apoptosis in breast cancer cells. | flavonoids, polyphenolic phytochemicals which include flavones and isoflavones, are present in the common human diet. it has been suggested that these compounds may exert anticancer activity; however, the mechanisms involved remain unknown. we have recently shown (sergeev, 2004, biochem biophys res commun 321: 462-467) that isoflavones can activate the novel apoptotic pathway mediated by cellular ca(2+). here, we report that polymethoxyflavones (pmfs) derived from sweet orange (citrus sinensis l ... | 2006 | 17046027 |
| effect of storage and processing on plasmid, yeast and plant genomic dna stability in juice from genetically modified oranges. | recombinant dna technology is an important tool in the development of plant varieties with new favourable features. there is strong opposition towards this technology due to the potential risk of horizontal gene transfer between genetically modified plant material and food-associated bacteria, especially if genes for antibiotic resistance are involved. since horizontal transfer efficiency depends on size and length of homologous sequences, we investigated the effect of conditions required for or ... | 2007 | 17064805 |
| determination of polymethoxylated flavones in peels of selected jamaican and mexican citrus (citrus spp.) cultivars by high-performance liquid chromatography. | the concentrations of the polymethoxylated flavones (pmfs) in peels of selected citrus cultivars grown in jamaica and mexico were determined. the pmfs were extracted from sun-dried citrus peels with reagent-grade methanol. analyses were carried out by reverse-phase hplc and uv detection. the column used was a c(18) 5 microm (150 x 4.6 mm) discovery column. elution was in the gradient mode, using a ternary mobile phase. the results showed that all the citrus cultivars used contained at least thre ... | 2007 | 17080505 |
| the effect of lemon, orange and bergamot essential oils and their components on the survival of campylobacter jejuni, escherichia coli o157, listeria monocytogenes, bacillus cereus and staphylococcus aureus in vitro and in food systems. | to investigate the effectiveness of oils and vapours of lemon (citrus limon), sweet orange (citrus sinensis) and bergamot (citrus bergamia) and their components against a number of common foodborne pathogens. | 2006 | 17105553 |
| an effective and low-cost culture medium for isolation and growth of xylella fastidiosa from citrus and coffee plants. | buffered charcoal-yeast extract medium (bcye) has been used for isolation of xylella fastidiosa from citrus (citrus sinensis) and coffee (coffea arabica) plants affected by citrus variegated chlorosis (cvc) and coffee leaf scorch (cls). bcye is composed of aces (2-[2-amino-2oxoethyl) amino]-ethanesulfonic acid) buffer, activated charcoal, yeast extract, l-cysteine, ferric pyrophosphate, and agar. aces buffer is costly and not always commercially available in brazil, and the l-cysteine and ferric ... | 2006 | 17111277 |
| diversity of flies (diptera: tephritidae and lonchaeidae) in organic citrus orchards in the vale do rio caí, rio grande do sul, southern brazil. | this study was carried out in the counties of montenegro and pareci novo located in the region of the vale do rio cai, rio grande do sul, southern brazil, aiming to determine the fruit fly species of tephritidae and lonchaeidae that occur in organic orchards of sweet orange [citrus sinensis (l.) osb.] cultivar céu, and murcott tangor (citrus reticulata blanco x c. sinensis), during the fruit ripening stages in 2003 and 2004. eight mcphail traps baited with integral grape juice diluted to 25% wer ... | 2006 | 17144140 |
| protein changes in the albedo of citrus fruits on postharvesting storage. | in this work, major protein changes in the albedo of the fruit peel of murcott tangor (tangerine x sweet orange) during postharvest ageing were studied through 2d page. protein content in matured on-tree fruits and in fruits stored in nonstressing [99% relative humidity (rh) and 25 degrees c], cold (99% rh and 4 degrees c), and drought (60% rh and 25 degrees c) conditions was initially determined. protein identification through ms/ms determinations revealed in all samples analyzed the occurrence ... | 2007 | 17910511 |
| a ptha homolog from xanthomonas axonopodis pv. citri responsible for host-specific suppression of virulence. | strains of the plant-pathogenic bacterium xanthomonas axonopodis pv. citri are differentiated into two groups with respect to aggressiveness (normal and weak) on citrus grandis cultivars but not on other citrus species such as citrus sinensis. random mutagenesis using the transposon tn5 in x. axonopodis pv. citri strain kc21, which showed weak aggressiveness on a c. grandis cultivar, was used to isolate mutant kc21t46, which regained a normal level of aggressiveness on the cultivar. the gene ina ... | 2007 | 17293422 |
| the endophyte curtobacterium flaccumfaciens reduces symptoms caused by xylella fastidiosa in catharanthus roseus. | citrus variegated chlorosis (cvc) is a disease of the sweet orange [citrus sinensis (l.)], which is caused by xylella fastidiosa subsp. pauca, a phytopathogenic bacterium that has been shown to infect all sweet orange cultivars. sweet orange trees have been occasionally observed to be infected by xylella fastidiosa without evidencing severe disease symptoms, whereas other trees in the same grove may exhibit severe disease symptoms. the principal endophytic bacterial species isolated from such cv ... | 2007 | 17978797 |
| preferential accumulation of severe variants of citrus tristeza virus in plants co-inoculated with mild and severe variants. | the viral population in sweet orange plants, either healthy or pre-inoculated with the asymptomatic isolate of citrus tristeza virus (ctv) t32, and then graft- or aphid-inoculated with the stem-pitting isolate t318, was characterized with respect to symptom expression, reaction with monoclonal antibody mca13, single-strand conformation polymorphism (sscp) of genes p18 and p20, bi-directional rt-pcr, and dot-blot hybridisation. all plants inoculated with t318, with or without pre-inoculation, sho ... | 2007 | 17294089 |
| karyotype diversity and the origin of grapefruit. | grapefruit is a group of citrus of recent origin, probably resulting from a cross between pummelo and sweet-orange. aiming to investigate this putative origin and the genetic variability among grapefruit cultivars, the karyotype of six grapefruits, two pummelos, and one tangelo cultivar (grapefruit x tangerine) were analyzed using sequential cma/dapi double staining and fish with rdna probes. the karyotypes of grapefruit 'duncan' and 'foster' differ from those of 'flame', 'henderson', 'marsh' an ... | 2007 | 17295131 |
| induced parthenogenesis in mandarin for haploid production: induction procedures and genetic analysis of plantlets. | this study focused on haploid induction in mandarin through in situ gynogenesis by pollination with irradiated pollen of 'meyer' lemon. pollination was carried out for three genotypes of mandarin with four levels of gamma-ray-irradiated pollen (150, 300, 600, and 900 gy). the resulting seeds were characterised by a small size. embryos were rescued in vitro and the ploidy level of the plantlets was determined by flow cytometry analysis. haploid, diploid, triploid plantlets were obtained. the hapl ... | 2007 | 17318461 |
| [spatial distribution of oncometopiafacialis (signoret) (hemiptera: cicadellidae) in citrus orchard]. | the spatial distribution of oncometopia facialis (signoret) was studied in sweet orange to elaborate sampling plans and decision-making procedure for the control of this sharpshooter in the field. the samplings were carried out fortnightly in a grid design disposition of 100 points (plants) in citrus orchard, with yellow sticky traps. the average number of o. facialis ranged from 0.35 to 1.17 insects/trap, with the i index varying from 0.89 to 1.82. the fit tests to negative binomial, poisson di ... | 2006 | 17352074 |
| chlorophyllase is a rate-limiting enzyme in chlorophyll catabolism and is posttranslationally regulated. | chlorophyll is a central player in harvesting light energy for photosynthesis, yet the rate-limiting steps of chlorophyll catabolism and the regulation of the catabolic enzymes remain unresolved. to study the role and regulation of chlorophyllase (chlase), the first enzyme of the chlorophyll catabolic pathway, we expressed precursor and mature versions of citrus (citrus sinensis) chlase in two heterologous plant systems: (1) squash (cucurbita pepo) plants using a viral vector expression system; ... | 2007 | 17369368 |
| thermal degradation of antioxidant micronutrients in citrus juice: kinetics and newly formed compounds. | the thermal degradation kinetics of vitamin c, two carotenoids (beta-carotene and beta-cryptoxanthin), and hesperidin, as a function of temperature, were determined for citrus juice [citrus sinensis (l.) osbeck and citrus clementina hort. ex tan]. the influence of dissolved oxygen on the rate of ascorbic acid degradation was also assessed. analysis of kinetic data suggested a first-order reaction for the degradation of vitamin c and carotenoids. the kinetics parameters dtheta, z, and ea have bee ... | 2007 | 17451252 |
| the survival of three strains of arcobacter butzleri in the presence of lemon, orange and bergamot essential oils and their components in vitro and on food. | to test the effect of oils and vapours of lemon, sweet orange and bergamot and their components against three arcobacter butzleri strains. | 2007 | 17451515 |
| etiology of three recent diseases of citrus in são paulo state: sudden death, variegated chlorosis and huanglongbing. | the state of são paulo (ssp) is the first sweet orange growing region in the world. yet, the ssp citrus industry has been, and still is, under constant attack from various diseases. in the 1940s, tristeza-quick decline (t-qd) was responsible for the death of 9 million trees in ssp. the causal agent was a new virus, citrus tristeza virus (ctv). the virus was efficiently spread by aphid vectors, and killed most of the trees grafted on sour orange rootstock. control of the disease resided in replac ... | 2007 | 17505974 |
| comparison of supercritical fluid chromatography and liquid chromatography for the separation of urinary metabolites of nobiletin with chiral and non-chiral stationary phases. | nobiletin (nob), a polymethoxylated flavone found in sweet orange (citrus sinensis) peel, is currently recognized as a promising anti-inflammatory and anti-tumor agent. it is believed that, by undergoing metabolic biotransformation in vivo, nobiletin is demethylated by hepatic p450 enzymes, yielding multiple hydroxylated metabolites. however, it has not been possible to date to separate the two demethylated nobiletin metabolites, 3'-demethyl-nob and 4'-demethyl-nob (regio-isomers) on reversed-ph ... | 2006 | 16799930 |
| [population fluctuation of sharpshooters vectors of xylella fastidiosa wells et al. in commercial citrus groves in northwestern paraná state]. | the citrus variegated chlorosis (cvc), an important disease of citrus in brazil, is caused by the bacterium xylella fastidiosa wells et al. and transmitted by xylem-feeding sharpshooters (hemiptera: cicadellidae). this study evaluated the fluctuation of populations of species of sharpshooters belonging to the tribes cicadellini and proconiini, from subfamily cicadelinae, in a commercial sweet orange [citrus sinensis (l.) osb.] grove, located in the northwest region of paraná state, brazil, in fo ... | 2007 | 17607459 |
| delay expression of limonoid udp-glucosyltransferase makes delayed bitterness in citrus. | genes encoding limonoid udp-glucosyltransferase from albedo of six citrus species with different levels of delayed bitterness are isolated and cloned in vector ptz57r/t. our results indicate that gene sequence of sweet lime (with intense juice delayed bitterness) have complete identity with satsuma mandarin (without distinctive juice delayed bitterness). also gene sequence of marsh seedless grapefruit, local orange and thompson navel orange (with mild juice delayed bitterness) have very similari ... | 2008 | 18407832 |
| effects of citrus sinensis (l.) osbeck epicarp essential oil on growth and morphogenesis of aspergillus niger (l.) van tieghem. | essential oils from different plant parts are known for their antimicrobial activity but the antifungal effects of essential oil from citrus sinensis (l.) osbeck epicarp on growth and morphogenesis of aspergillus niger has not been observed so far. the mycelial growth was inhibited at 2.5 and 3.0 microg/ml of oil in potato dextrose broth and agar medium, respectively. these concentrations were fungicidal under the test conditions. the fungitoxicity of oil did not change even at exposure to 100 d ... | 2008 | 16870411 |
| recombinant expression and characterization of a xylella fastidiosa cysteine protease differentially expressed in a nonpathogenic strain. | xylella fastidiosa is a xylem-limited, gram-negative bacterium responsible for citrus variegated chlorosis (cvc) in sweet oranges. in the present study, we present the recombinant expression, purification and characterization of an x. fastidiosa cysteine protease (dubbed xylellain). the recombinant xylellain ((his)xylellain) was able to hydrolyze carbobenzoxy-phe-arg-7-amido-4-methylcoumarin (z-fr-mca) and carbobenzoxy-arg-arg-7-amido-4-methylcoumarin (z-rr-mca) with similar catalytic efficienci ... | 2006 | 16907719 |
| antiproliferative activity of citrus juices and hplc evaluation of their flavonoid composition. | the antiproliferative activity of fresh fruit juices extracted from citrus sinensis (cv. washington navel and cv. sanguinello), c. deliciosa cv. avana, c. clementina cv. nules, c. aurantium subsp. myrtifolia , was evaluated against k562 (human chronic myelogenous leukemia), hl-60 (human leukemia) and mcf-7 (human breast adenocarcinoma) cell lines. all the juices tested showed antiproliferative activity. moreover, the pattern of the main flavanone compounds in the juices has been determined by hp ... | 2007 | 17628348 |
| difference of a citrus late-ripening mutant (citrus sinensis) from its parental line in sugar and acid metabolism at the fruit ripening stage. | 'fengjiewancheng' (fw) (citrus sinensis), a bud sport of 'fengjie 72-1' navel orange (fj), ripens one month later than its parental line. differences in sugar and acid content and the transcript level of sucrose-and citric-metabolic enzymes for the two cultivars were investigated during fruit ripening. results showed that both sugar and acid metabolisms of the mutant were affected by the mutation. in the pulp of fw, sugar content was significantly lower than that in fj before 227 daf (days after ... | 2007 | 17653673 |
| development of genetic maps of the citrus varieties 'murcott' tangor and 'pera' sweet orange by using fluorescent aflp markers. | the progeny of 87 bc(1) hybrids of 'murcott' tangor and 'pera' sweet orange, genotyped with fluorescent amplified fragment length polymorphism (faflp) markers, was used for the construction of genetic maps for both citrus varieties. mapping strategies, considering the progeny as a result of backcrossing and cross-pollination, were exploited in mapmaker 2.0 (lod score >or= 3.0 and <or= 0.40) and joinmap 3.0 software (lod score >or= 3.0 and theta <or = 0.25), respectively. genetic map distances (i ... | 2007 | 17666774 |
| cross-correlation patterns of air and soil temperatures, rainfall and diaprepes abbreviatus root weevil in citrus. | time series cross-correlation analysis is appropriate when measuring relationships between two different time series. using this approach, the authors quantified the relationship between the time series air temperature (at), soil temperature (st), rainfall, relative humidity (rh) and diaprepes abbreviatus (l.) (coleoptera: curculionidae) root weevil across a period of 30 months, and examined how closely the distribution of diaprepes root weevil was related to at, st, rainfall and rh within this ... | 2007 | 17674428 |
| genetic transformation of sweet orange with the coat protein gene of citrus psorosis virus and evaluation of resistance against the virus. | citrus psorosis is a serious viral disease affecting citrus trees in many countries. its causal agent is citrus psorosis virus (cpsv), the type member of genus ophiovirus. cpsv infects most important citrus varieties, including oranges, mandarins and grapefruits, as well as hybrids and citrus relatives used as rootstocks. certification programs have not been sufficient to control the disease and no sources of natural resistance have been found. pathogen-derived resistance (pdr) can provide an ef ... | 2008 | 17712560 |
| potential perchlorate exposure from citrus sp. irrigated with contaminated water. | citrus produced in the southwestern united states is often irrigated with perchlorate-contaminated water. this irrigation water includes colorado river water which is contaminated with perchlorate from a manufacturing plant previously located near the las vegas wash, and ground water from wells in riverside and san bernardino counties of california which are affected by a perchlorate plume associated with an aerospace facility once located near redlands, california. studies were conducted to eva ... | 2006 | 17723376 |
| transmission efficiency of xylella fastidiosa by sharpshooters (hemiptera: cicadellidae) in coffee and citrus. | xylella fastidiosa (wells, raju, hung, weisburg, mandelco-paul, and brenner) is a bacterial pathogen transmitted by several sharpshooters in two tribes of cicadellinae (proconiini and cicadellini). here, we compared the transmission efficiency of x. fastidiosa in coffee (coffea arabica l.) and citrus [citrus sinensis (l.) osbeck] by cicadellini [bucephalogonia xanthophis (berg) and dilobopterus costalimai young] and proconiini [homalodisca ignorata melichar and oncometopia facialis (signoret)] s ... | 2008 | 18767717 |
| activity against drug resistant-tuberculosis strains of plants used in mexican traditional medicine to treat tuberculosis and other respiratory diseases. | tuberculosis (tb) kills about 3 million people per year worldwide. furthermore, tb is an infectious disease associated with hiv patients, and there is a rise in multidrug-resistant tb (mdr-tb) cases around the world. there is a need for new anti-tb agents. the study evaluated the antimycobacterial activity of nine plants used in mexican traditional medicine to treat tuberculosis and other respiratory diseases. nasturtium officinale showed the best activity (mic = 100 microg/ml) against the sensi ... | 2008 | 17726732 |
| antidiabetic potential of citrus sinensis and punica granatum peel extracts in alloxan treated male mice. | an investigation on the effects of four different concentrations of peel extract from citrus sinensis (cs) or punica granatum (pg) in male mice revealed the maximum glucose lowering and antiperoxidative activities at 25 mg/kg of cs and 200 mg/kg of pg. in a separate experiment their potential was evaluated with respect to the regulation of alloxan induced diabetes mellitus. while a single dose of alloxan (120 mg/kg) increased the serum levels of glucose and alpha-amylase activity, rate of water ... | 2007 | 18806305 |
| inhibitory effect of citrus 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone on 12-o-tetradecanoylphorbol 13-acetate-induced skin inflammation and tumor promotion in mice. | 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone (5-oh-hxmf), a polymethoxyflavone, is found exclusively in the citrus genus, particularly in the peels of sweet orange. herein, we report the first investigation of the inhibitory effects of 5-oh-hxmf on 12-o-tetradecanoylphorbol-13-acetate (tpa)-induced expression of inducible nitric oxide synthase (inos) and cyclooxygenase-2 (cox-2) in mouse skin. we found that the topical application of 5-oh-hxmf can effectively inhibit the transcriptional activation ... | 2007 | 17962218 |
| apoptosis-inducing activity of hydroxylated polymethoxyflavones and polymethoxyflavones from orange peel in human breast cancer cells. | sweet orange (citrus sinensis l.) peel is a rich resource of flavonoids, especially polymethoxyflavones (pmfs). citrus flavonoids exert a broad spectrum of biological activity, including antiproliferative and proapoptotic effects in cancer cells. we have recently shown that individual pmfs from orange peel induce ca(2+)-mediated apoptosis in human breast cancer cells and that hydroxylation of pmfs is critical for enhancing their proapoptotic activity. here, we report that the fraction of orange ... | 2007 | 17979096 |
| pathogenic interactions between xanthomonas axonopodis pv. citri and cultivars of pummelo (citrus grandis). | abstract the aggressiveness of strains of xanthomonas axonopodis pv. citri on seven citrus species, including citrus sinensis (navel orange), c. paradisi (grapefruit), c. unshiu (satsuma mandarin), c. junos (yuzu), c. aurantifolia ('mexican' lime), c. tachibana (tachibana), and c. grandis (pummelo: 'otachibana', 'banpeiyu', and 'anseikan'), were assessed by comparing lesion expansion and growth in planta, using a prick inoculation method. the existence of two groups distinct in aggressiveness wa ... | 2000 | 18943380 |
| structure and composition of the assemblage of parasitoids associated to phyllocnistis citrella pupae stainton (lepidoptera: gracillariidae) in citrus orchards in southern brazil. | the structure and composition of the assemblage of pupal parasitoids of phyllocnistis citrella stainton, the citrus leafminer, were studied in two citrus orchards (citrus deliciosa tenore cv. montenegrina and citrus sinensis (l.) osbeck x citrus reticulata blanco hybrid murcott), in montenegro county (29 degrees 68's and 51 degrees 46'w), southern brazil. at fortnightly samplings, from july 2001 to june 2003, all the new shoots from 24 randomly selected trees were inspected. the species richness ... | 2007 | 18060301 |
| characterization of the most odor-active volatiles of orange wine made from a turkish cv. kozan (citrus sinensis l. osbeck). | the aroma-active compounds of cv. turkish kozan orange wine were analyzed by sensory and instrumental analyses. liquid-liquid extraction with dichloromethane was used for extraction of volatile components. according to sensory analysis, the aromatic extract obtained by liquid-liquid extraction was representative of orange wine odor. a total of 63 compounds were identified and quantified in orange wine. the results of the gas chromatography-olfactometry analysis showed that 35 odorous compounds w ... | 2008 | 18078317 |
| inheritance and heritability of resistance to citrus leprosis. | abstract the genetic inheritance of resistance to leprosis, the most important viral disease of citrus in brazil, was characterized through the phenotypic assessment of 143 hybrids resulting from crosses between tangor 'murcott' (citrus sinensis x c. reticulata) and sweet orange 'pêra' (c. sinensis), considered to be resistant and susceptible to the disease, respectively. all plants were grafted onto rangpur lime (c. limonia) and inoculated with citrus leprosis virus, cytoplasmic type through th ... | 2006 | 18943497 |
| boron deficiency decreases growth and photosynthesis, and increases starch and hexoses in leaves of citrus seedlings. | seedlings of sweet orange (citrus sinensis) were fertilized for 14 weeks with boron (b)-free or b-sufficient (2.5 or 10 microm h(3)bo(3)) nutrient solution every other day. boron deficiency resulted in an overall inhibition of plant growth, with a reduction in root, stem and leaf dry weight (dw). boron-starved leaves showed decreased co(2) assimilation and stomatal conductance, but increased intercellular co(2) concentrations. activities of ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisc ... | 2008 | 18191499 |
| genetic diversity of xylella fastidiosa strains from costa rica, são paulo, brazil, and united states. | abstract the diversity of 42 xylella fastidiosa strains from costa rica, são paulo, brazil, and the united states were analyzed using the sequence of the 16s rrna gene by variable number of tandem repeat (vntr) fragment analysis and by restriction fragment length polymorphisms (rflp) of a specific polymerase chain reaction (pcr)-amplification product using enzyme cfoi. limited variability in the sequence of the 16s rrna gene was observed and, although the separation was not absolute, most strain ... | 2007 | 18943693 |
| diversity and infectivity of arbuscular mycorrhizal fungi in agricultural soils of the sichuan province of mainland china. | knowledge about the presence and diversity of arbuscular mycorrhizal fungi (amf) in a specific area is an essential first step for utilizing these fungi in any application. the community composition of amf in intensively managed agricultural soil in the sichuan province of southwest china currently is unknown. in one set of samples, amf were trapped in pot cultures from 40 fields growing legumes in the panxi region, southeast sichuan. in a second set of samples, the mpn method with four-fold dil ... | 2008 | 18224350 |
| functional characterization of ap3, soc1 and wus homologues from citrus (citrus sinensis). | flowering and flower formation are defining features of angiosperms and the control of these developmental processes involves a common repertoire of genes which are shared among different species of flowering plants. these genes were first identified using various homeotic and flowering time mutants of arabidopsis and snapdragon, and homologous genes have subsequently been isolated from a wide range of different plant species based on the conservation of protein sequence and function. using dege ... | 2007 | 18251886 |
| juice quality of two new mandarin-like hybrids (citrus clementina hort. ex tan x citrus sinensis l. osbeck) containing anthocyanins. | tacle and clara [monreal clementine (citrus clementina hort. ex tan) x tarocco orange (citrus sinensis l. osbeck)] are two new triploid citrus hybrids developed by the cra-istituto sperimentale per l'agrumicoltura (acireale, italy). the fruits are easy-peeling and juicy, have a pleasant taste like the tarocco orange, and are sweet like the monreal clementine. in addition, a distinctively attractive characteristic of these mandarin-like fruits is the red-pigmented flesh caused by the presence of ... | 2008 | 18298061 |
| postbloom fruit drop of citrus and key lime anthracnose are caused by distinct phylogenetic lineages of colletotrichum acutatum. | colletotrichum acutatum causes two diseases of citrus, postbloom fruit drop (pfd) and key lime anthracnose (kla). pfd is a disease restricted to flowers of sweet orange and most other citrus, and symptoms include petal necrosis, abscission of developing fruit, and the formation of persistent calyces. kla is a disease of foliage, flowers, and fruits of key lime only, and symptoms include necrotic lesions on leaves, fruits, twigs, flowers, and blight of entire shoots. the internal transcribed spac ... | 2008 | 18944086 |
| evaluation of selection strategies alternative to nptii in genetic transformation of citrus. | the neomycin phosphotransferase (nptii) selection system has proved successful in citrus transformation; however, it may be recommendable to replace it given the pressure exerted against antibiotic-resistance selectable marker genes in transgenic plants. the present work investigates three different selection alternatives, comparing them to nptii selection in two citrus genotypes, carrizo citrange and pineapple sweet orange. the first method used the beta-glucuronidase (uida) reporter marker gen ... | 2008 | 18317775 |
| quantification of xylella fastidiosa from citrus trees by real-time polymerase chain reaction assay. | abstract xylella fastidiosa is the causal agent of citrus variegated chlorosis (cvc), a destructive disease of sweet orange cultivars in brazil. polymerase chain reaction (pcr)-based assays constitute the principal diagnostic method for detection of these bacteria. in this work, we established a real-time quantitative pcr (qpcr) assay to quantify x. fastidiosa in naturally and artificially infected citrus. the x. fastidiosa cell number detected in the leaves increased according to the age of the ... | 2002 | 18944214 |
| antioxidant enzymatic activity is linked to waterlogging stress tolerance in citrus. | soil flooding constitutes a seasonal factor that negatively affects plant performance and crop yields. in this work, the relationship between oxidative damage and flooding sensitivity was addressed in three citrus genotypes with different abilities to tolerate waterlogging. we examined leaf visible damage, oxidative damage in terms of malondialdehyde (mda) concentration, leaf proline concentration, leaf and root ascorbate and glutathione contents and the antioxidant enzyme activities superoxide ... | 2008 | 18333999 |
| leaf water relations and net gas exchange responses of salinized carrizo citrange seedlings during drought stress and recovery. | since salinity and drought stress can occur together, an assessment was made of their interacting effects on leaf water relations, osmotic adjustment and net gas exchange in seedlings of the relatively chloride-sensitive carrizo citrange, citrus sinensis x poncirus trifoliata. | 2007 | 17575285 |
| distribution and quantification of candidatus liberibacter americanus, agent of huanglongbing disease of citrus in são paulo state, brasil, in leaves of an affected sweet orange tree as determined by pcr. | huanglongbing (hlb), an insect-transmitted disease of citrus, known for many years in asia and africa, has appeared in the state of são paulo state (ssp), brazil, in 2004, and the state of florida, usa, in 2005. hlb endangers the very existence of citrus, as trees infected with the bacterial pathogen, irrevocably decline. in the absence of curative procedures, control of hlb is difficult and only based on prevention. even though not available in culture, the hlb bacterium could be shown to be gr ... | 2008 | 18400468 |
| antiperoxidative, antithyroidal, antihyperglycemic and cardioprotective role of citrus sinensis peel extract in male mice. | an extract of citrus sinensis (cs) peel was evaluated for its efficacy in ameliorating l-thyroxine (l-t(4)) induced tissue lipid peroxidation (lpo), hyperthyroidism and hyperglycemia in mice. in a preliminary investigation, of the three different doses of cs (12.5 mg/kg, 25 mg/kg and 50.0 mg/kg) peel extract, 25 mg/kg was found to be the most effective and antiperoxidative, while 50 mg/kg was proved to be hepatotoxic. therefore in the pilot experiment the effects of 25 mg/kg/day of cs for 10 day ... | 2008 | 18412146 |
| argyrotaenia sphaleropa (meyrick) (lepidoptera: tortricidae) in citrus in the state of paraná, brazil. | argyrotaenia sphaleropa (meyrick) is associated with various fruit species in south america. this tortricid was first detected in citrus, citrus sinensis (l.) osbeck, in brazil, in commercial groves in northern paraná, during the 1994/95-crop season. the aim of this work was to study the biology of this lepidopteran under laboratory conditions (28 +/- 2 degrees c, 70 +/-10% ur, 14h photofase) using the citrus cultivar pêra. larvae obtained from egg masses collected in a commercial citrus grove i ... | 2007 | 17607470 |
| xylella fastidiosa disturbs nitrogen metabolism and causes a stress response in sweet orange citrus sinensis cv. pera. | xylella fastidiosa (xf) is a fastidious bacterium that grows exclusively in the xylem of several important crop species, including grape and sweet orange (citrus sinensis l. osb.) causing pierce disease and citrus variegated chlorosis (cvc), respectively. the aim of this work was to study the nitrogen metabolism of a highly susceptible variety of sweet orange cv. 'pêra' (c. sinensis l. osbeck) infected with xf. plants were artificially infected and maintained in the greenhouse until they have de ... | 2007 | 17609534 |
| campylobacter and arcobacter species sensitivity to commercial orange oil fractions. | seven orange oil fractions were screened for their ability to inhibit the growth of selected campylobacter and arcobacter spp. using the standard agar-disk diffusion assay. cold pressed (cp) terpeneless valencia orange oil was found to be the most inhibitory to both campylobacter jejuni and campylobacter coli, exhibiting maximum zones of inhibition up to 80+/-0.0 mm. five-fold concentrated valencia oil and distilled d-limonene resulted in campylobacter inhibition zones ranging from 11.0+/-1.4 to ... | 2009 | 19070381 |
| spatial distribution of phyllocnistis citrella stainton (lepidoptera: gracillariidae) larvae in three scales. | the spatial distribution of larvae of phyllocnistis citrella stainton (lepidoptera: gracillariidae), the citrus leaf miner, in leaves, shoots, and tree crowns of montenegrina tangerine (citrus deliciosa tenore) and murcott tangor (citrus sinensis l. osbeck x citrus reticulata blanco) was determined. fortnightly samplings from july/2001 to june/2003, in orchards located in montenegro (29 degrees 68' s and 51 degrees 46' w), rs, brazil, were carried out. at each sampling occasion eighth shoots ran ... | 2008 | 18470399 |
| rapd-based genetic analysis of offsprings from the sexual cross using allotetraploid citrus somatic hybrid as pollen parent. | thirty-one polymorphic decamer primers were selected to genotype 92 progenies from the cross between yiben no.4, a monoembryonic diploid f1 hybrid of citrus reticulata blanco cv huanongbendizao tangerine and c. ichangensis swingle, and [hamlin sweet orange + rough lemon], an allotetraploid somatic hybrid of citrus sinensis osbeck cv. hamlin and c. jambhiri lush cv. rough lemon. chi2 (chi-square) analysis of rapd markers in the progenies indicated they were randomly transmitted from the four dono ... | 2007 | 17609894 |
| a comparison of four dna extraction methods for the detection of citrus yellow mosaic badna virus from two species of citrus using pcr and dot-blot hybridization. | nucleic acid preparations extracted using four procedures were assessed to determine the suitability of the procedure for pcr-based and dna dot-blot-based detection of citrus yellow mosaic badna virus (cmbv) from two citrus species, acid lime and pummelo. it was found that the success of pcr detection depended upon the procedure of dna extraction whereas the dot-blot detection was successful with all extraction methods examined. cmbv dna sequences amplified from two citrus species indicated high ... | 2008 | 18582956 |
| medicinal values of fruit peels from citrus sinensis, punica granatum, and musa paradisiaca with respect to alterations in tissue lipid peroxidation and serum concentration of glucose, insulin, and thyroid hormones. | peel extracts from citrus sinensis, punica granatum, and musa paradisiaca were investigated for their effects on tissue lipid peroxidation (lpo) and on the concentration of thyroid hormones, insulin, and glucose in male rats. in vitro inhibition of h(2)o(2)-induced lpo in red blood cells of rats by 0.25, 0.50, 1.0, and 2.0 microg/ml c. sinensis, p. granatum, and m. paradisiaca peel extracts was observed in a dose-specific manner. maximum inhibition was observed at 0.50 microg/ml c. sinensis, 2.0 ... | 2008 | 18598183 |
| in vitro evaluation of the effect of natural orange juices on dentin morphology. | the patient's diet has been considered an important etiological factor of dentin hypersensitivity. the frequent ingestion of acidic substances can promote the loss of dental structure or remove the smear layer. the purpose of this study was to evaluate the degree of smear layer removal and dentinal tubules exposure by different natural orange juices. extracted human teeth were submitted to manual scaling in order to develop the smear layer. seventy dentin samples were obtained and distributed in ... | 2008 | 18622489 |
| chemical characterization of citrus sinensis grafted on c. limonia and the effect of some isolated compounds on the growth of xylella fastidiosa. | citrus sinensis grafted on c. limonia produces a considerable number of compounds that are not common in both plants developed from germination of seeds. the chemical profile of scion and rootstock differ notably for absence in the form of flavonoids and coumarins containing c5 prenyl groups attached to the carbon atoms of aromatic and heterocyclic systems or to oxygen. only linear pyranocoumarins xanthyletin and xanthoxyletin were found in scion. this observation indicates that the prenylated c ... | 2008 | 18683948 |
| the mechanism of action of a citrus oil blend against enterococcus faecium and enterococcus faecalis. | the aim was to explore the mechanisms by which a blend of orange (citrus sinensis) : bergamot (citrus bergamia) (1 : 1 v/v) eo (essential oil) (2% v/v) and its vapour (15 mg l(-1) air) brings about its antimicrobial effect against enterococcus faecium and enterococcus faecalis. | 2009 | 19187138 |
| gene expression analysis in citrus reveals the role of gibberellins on photosynthesis and stress. | the effect of gibberellins (ga) on internode transcriptome was investigated in transgenic carrizo citrange (citrus sinensis x poncirus trifoliata) plants overexpressing endogenous ccga20ox1 (encoding a ga biosynthetic gene), and in non-transformed explants treated with ga(3), using a citrus cdna microarray. substantial modulation of gene expression was found in sense ccga20ox plants. extensive up-regulation of genes involved in photosynthesis and carbon utilization, and down-regulation of those ... | 2008 | 18684239 |
| dispersion patterns and sampling plans for diaphorina citri (hemiptera: psyllidae) in citrus. | the abundance and spatial dispersion of diaphorina citri kuwayama (hemiptera: psyllidae) were studied in 34 grapefruit (citrus paradisi macfad.) and six sweet orange [citrus sinensis (l.) osbeck] orchards from march to august 2006 when the pest is more abundant in southern texas. although flush shoot infestation levels did not vary with host plant species, densities of d. citri eggs, nymphs, and adults were significantly higher on sweet orange than on grapefruit. d. citri immatures also were fou ... | 2008 | 18767763 |
| faunistic analysis of sharpshooters (hemiptera: auchenorrhyncha, cicadellidae) in a 'westin' sweet orange orchard. | the purpose of this study was to analyze sharpshooter fauna in a five-year-old 'westin' sweet orange orchard. yellow sticky traps were placed on the edge of a forest, and on the periphery and inside the citrus stand. the traps were evaluated fortnightly, for three years. the most frequent species were acrogonia citrina marucci & cavichioli, bucephalogonia xanthophis (berg), and oncometopia facialis (signoret). b. xanthophis occurred more in the forest edge, especially on spring and winter. a. ci ... | 2008 | 18813748 |
| disease development and symptom expression of xanthomonas axonopodis pv. citri in various citrus plant tissues. | abstract experimental inoculations of xanthomonas axonopodis pv. citri in different tissues of tahiti lime and pineapple sweet orange were conducted monthly under natural conditions on réunion island. the interactions between a set of environmental and epidemic variables associated with disease expression and 184 different factor combinations were investigated to determine the parameters needed to explain asiatic citrus canker (acc) disease expression. area under the disease progress curve (audp ... | 2003 | 18943164 |
| citrus viroid v: occurrence, host range, diagnosis, and identification of new variants. | the recently described citrus viroid v (cvd-v) has been proposed as a new species of the genus apscaviroid within the family pospiviroidae. analysis of 64 samples from different citrus-growing areas has shown that cvd-v is present in the united states, spain, nepal, and the sultanate of oman. cvd-v found in six sweet orange sources from the sultanate of oman was identical to the reference cvd-v variant, whereas three new variants with sequence identities of 98.6% (cvd-vca), 97.3% (cvd-vst), and ... | 2008 | 18943408 |
| proteomic analysis reveals suppression of bark chitinases and proteinase inhibitors in citrus plants affected by the citrus sudden death disease. | citrus sudden death (csd) is a disease of unknown etiology that greatly affects sweet oranges grafted on rangpur lime rootstock, the most important rootstock in brazilian citriculture. we performed a proteomic analysis to generate information related to this plant pathogen interaction. protein profiles from healthy, csd-affected and csd-tolerant stem barks, were generated using two-dimensional gel electrophoresis. the protein spots were well distributed over a pi range of 3.26 to 9.97 and a mole ... | 2008 | 18943454 |