Publications

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larvicidal and irritant activities of hexane leaf extracts of citrus sinensis against dengue vector aedes aegypti l.to assess the larvicidal and irritant activities of the hexane extracts of leaves of citrus sinensis (c. sinensis) against the early fourth instars and female adults of aedes aegypti (ae. aegypti).023569887
larvicidal activity of syzygium aromaticum (l.) merr and citrus sinensis (l.) osbeck essential oils and their antagonistic effects with temephos in resistant populations of aedes aegypti.environmentally friendly botanical larvicides are commonly considered as an alternative to synthetic larvicides against aedes aegypti linn. in addition, mosquito resistance to currently used larvicides has motivated research to find new compounds acting via different mechanisms of action, with the goal of controlling the spread of mosquitos. essential oils have been widely studied for this purpose. this work aims to evaluate the larvicidal potential of syzygium aromaticum and citrus sinensis ess ...027384083
carotenoid profiling of red navel orange "cara cara" harvested from five regions in china.understanding the composition and content of carotenoids in red-flesh navel orange "cara cara" cultivated in different regions is valuable for its nutrition assessment and fruit processing control. herein, the carotenoids of "cara cara" fruits, harvested from five typical citrus growing regions of china, were identified and quantified by hplc-dad coupled with hplc-dad-apci-ms/ms. in saponified extracts, 19 free carotenoids including 8 carotenes and 11 xanthophylls were identified. in unsaponifie ...028490141
differentiation using microwave plasma torch desorption mass spectrometry of navel oranges cultivated in neighboring habitats.the molecular fingerprinting of intact fruit samples combined with statistical data analysis can allow the assessment of fruit quality and location of origin. herein, microwave plasma torch desorption ionization mass spectrometry (mpt-ms) was applied to produce molecular fingerprints for the juice sac and exocarp of navel oranges cultivated in three closely located habitats, and the mass spectrometric fingerprints were differentiated by principal component analysis (pca). because of the relative ...028269986
comparative study on ambient ionization methods for direct analysis of navel orange tissues by mass spectrometry.it is of sustainable interest to improve the sensitivity and selectivity of the ionization process, especially for direct analysis of complex samples without matrix separation. herein, four ambient ionization methods including desorption atmospheric pressure chemical ionization (dapci), heat-assisted desorption atmospheric pressure chemical ionization (heat-assisted dapci), microwave plasma torch (mpt), and internal extractive electrospray ionization (ieesi) were employed for comparative analysi ...028628730
influence of orange cultivar and mandarin postharvest storage on polyphenols, ascorbic acid and antioxidant activity during gastrointestinal digestion.polyphenols, ascorbic acid content and antioxidant activity of two sweet oranges (navel-n and cara cara-cc) and mandarin (clementine-m) as well as their bioaccessibilities were evaluated in pulps and compared to those in fresh juice. thus, pulps of oranges and mandarins displayed higher hesperidin (hes), narirutin (nar), total flavonoids (tf), total phenols (tp) and antioxidant activity (aac) than their corresponding juices. also, cc products presented higher bioactive compounds content than n o ...028193404
led blue light-induced changes in phenolics and ethylene in citrus fruit: implication in elicited resistance against penicillium digitatum infection.the objective was to investigate whether led blue light (lbl) induces changes in phenolics and ethylene production of sweet oranges, and whether they participate in lbl-elicited resistance against the most important postharvest pathogen (penicillium digitatum) of citrus fruit. the expression of relevant genes of the phenylpropanoid and ethylene biosynthetic pathways during elicitation of resistance was also determined. different lbl (wavelength 450nm) quantum fluxes were used within the 60-630μm ...027719952
influence of postharvest treatments on qualitative and chemical parameters of tarocco blood orange fruits to be used for fresh chilled juice.tarocco "sant'alfio" is a late ripening blood orange cultivar. blood oranges are more and more appreciated from consumers for their high nutraceutical value due to the presence of bioactive compounds including vitamin c, polyphenols, flavonoids and hydroxycinnamic acids. the aim of this work is to set up a reliable protocol for postharvest storage of the very-late tarocco "sant'alfio" orange to prolong the availability of this product in the market to be used for fresh chilled orange juice produ ...028407933
qtl mapping for fruit quality in citrus using dartseq markers.citrus breeding programs have many limitations associated with the species biology and physiology, requiring the incorporation of new biotechnological tools to provide new breeding possibilities. diversity arrays technology (dart) markers, combined with next-generation sequencing, have wide applicability in the construction of high-resolution genetic maps and in quantitative trait locus (qtl) mapping. this study aimed to construct an integrated genetic map using full-sib progeny derived from mur ...028403819
[influence of spectral pre-processing on pls quantitative model of detecting cu in navel orange by libs].cu in navel orange was detected rapidly by laser-induced breakdown spectroscopy (libs) combined with partial least squares (pls) for quantitative analysis, then the effect on the detection accuracy of the model with different spectral data ptetreatment methods was explored. spectral data for the 52 gannan navel orange samples were pretreated by different data smoothing, mean centralized and standard normal variable transform. then 319~338 nm wavelength section containing characteristic spectral ...026415466
absorption of hydrocyanic acid by valencia-orange fruits and leaves fumigated at atmospheric pressure and at partial vacuum. 194516653971
ether-soluble organic acids of mature valencia orange leaves. 194716654097
the influence of rootstock on the mineral composition of valencia orange leaves. 194916654236
the response of young valencia orange trees to differential boron supply in sand culture. 195116654340
[recurrent dermatitis of the hands caused by contact with oranges; intolerance to sweet orange juice]. 195313118905
effect of differential nitrogen, potassium, and magnesium supply to young valencia orange trees in sand culture on mineral composition especially of leaves and fibrous roots. 195416654674
oil of sweet orange: a possible role in carcinogenesis. 195913662498
purification and identification of flavanone glycosides in the peel of the sweet orange. 196013818662
cyanide & chelating agent effects on in vitro co(2) fixation in sweet orange leaves. 196116655460
nondestructive method for estimating chlorophyll content of leaves.a quantitative relationship is shown to exist between the chlorophyll content of soybean and valencia orange leaves and their percentage reflectance of light of wavelength 625 mmicro as measured by a colorimeter with reflectance attachment.196117754654
effects of calcium, potassium, & magnesium on oxalic, malic, & citric acid content of valencia orange leaf tissue. 196116655480
regreening of valencia orange as influenced by potassium gibberellate. 196216655704
subcellular distribution of fluoride in navel orange leaves. 19655850916
isolation and identification of alcohols in cold-pressed valencia orange oil by liquid-liquid extraction and gas chromatography. 196514274120
site of fluoride accumulation in navel orange leaves.fluoride-polluted navel orange leaves, citrus sinensis (linn.) osbeck, were fractionated into the subcellular components in hexane/carbon tetrachloride mixtures having various densities. fluoride was determined at each fraction. analyses were also made for the subcellular distribution of chlorophyll, nitrogen, and dna to assess the extent of cross-contamination of each component. the fraction containing cell wall, nuclei, and partly broken cells apparently contained a major amount of fluoride. h ...19665908632
pyrolysis-gas chromatographic method for polybutene residues on navel oranges. 196824190082
constituents of valencia oranges. 19695795080
infectivity of two biotypes of the citrus nematode on citrus and on some other hosts.the infectivity and development of two biotypes of citrus nematode (tylenchulus semipenetrans) were compared on highly resistant poncirus trifoliata selection 'pomeroy,' moderately susceptible 'troyer' citrange, and highly susceptible sweet orange selection 'homosassa' small seedlings in a glasshouse. biotype-1 was more infective on the above hosts and developed faster on sweet orange and on 'troyer' citrange than biotype-2. the differences in infectivity were interpreted to reflect differences ...196919325669
persistence of azodrin residues on and in valencia oranges and in laboratory-processed citrus pulp cattle feed. 19705474246
water potential components in growing citrus fruits.growing navel orange fruits (citrus sinensis) 5.4 to 5.7 centimeters in diameter were used as a model system to determine the effects of transpiration and carbohydrate translocation on water and osmotic potentials in fruit tissues. evidence supported the hypothesis that osmotic potential in the vesicles would be affected little by changes in transpiration or carbohydrate translocation because the vesicles are anatomically isolated from the transpiration stream and are at the end of the carbohydr ...197016657407
extensibility of pericarp tissue in growing citrus fruits.the tensile force existing in the pericarp of a growing citrus (citrus sinensis) fruit 17 to 19 centimeters in circumference was sufficiently high to cause a 3% shrinkage of the pericarp when it was excised. when a fruit was cut along the equator to the central axis, shrinkage of the pericarp resulted in the formation of a wedge-shaped gap at the cut. stretch modulus of the pericarp was determined by measuring the force required to stretch excised strips of tissue to 1% longer than their excised ...197016657546
respiratory response, ethylene production, and response to ethylene of citrus fruit during ontogeny.the initial respiratory rates at 20 centrigrade of detached oranges (valencia and navel), grapefruit, and lemons decreased during ontogeny. small attached oranges respired at the same rate as detached fruits of the same weight, and cutting the pedicel produced no shock or injury stimulus to the respiratory rate. small oranges and grapefruit (average weight about 15 grams) showed pseudoclimacteric respiratory patterns and produced ethylene. the height of the respiratory rise and the amount of eth ...197016657319
scanning electron-microscopy of abscission-zone surfaces of valencia-orange fruit.the scanning electron-microscope was used to obtain a more adequate view of the separation surfaces exposed when the stem was pulled from the fruit of valencia orange. separations were made at 0, 24 and 48 h after ethylene treatment of the fruit. the most noticeable differences are the lessened amount of tearing in proximal vascular bundles at 48 h and decreased rupture of distal parenchyma cells first observed at 24 h.197124493280
peel oils of different types of citrus sinensis l. and c. aurantium l. growing in egypt. 19715572114
inhibition of murine subcutaneous and intravenous benzo(rst)pentaphene. carcinogenesis by sweet orange oils and d-limonene. 19715090417
persistence of omite residues on and in navel oranges and lemons and in laboratory-processed citrus pulp cattle feed. 19714332483
adventive plants from ovules and nucelli in citrus.1- to 8-week-old ovules and nucelli from three citrus cultivars-shamouti and valencia (citrus sinensis) oranges and marsh seedless (c. paradisi) grapefruit-were cultured in vitro. no embryo differentiation was observed in the explants prior to culture. the shamouti ovules had degenerated and were apparently unfertilized. embryoids formed on murashige and tucker nutrient medium supplemented with 500 mg/l malt extract. whole plants developed on the same basal medium supplemented with kinetin and i ...197224477995
changes in cellulase and pectinase activities in fruit tissues and separation zones of citrus treated with cycloheximide.cellulase activity increased in separation-zone tissues 1 day after "valencia" orange (citrus sinensis [l.] osbeck) was treated with 20 micrograms per milliliter cycloheximide. exocellulase was detected only in the separation zones of treated fruit, whereas endocellulase was present in zones from both treated and control fruit. endocellulase activity in separation-zone tissue of treated fruit was nearly three times as great as that in control tissues. cellulase activity was restricted to separat ...197316658383
diurnal variations in photosynthetic products and nitrogen metabolism in expanding leaves.expanding leaves of capsicum frutescens l. cv. california wonder, cucumis melo l. cv. hales best, and citrus sinensis l. osbeck cv. washington navel showed a marked diurnal periodicity in the incorporation of (14)c from photosynthetically fixed (14)co(2) into amino acids. incorporation was virtually nil at the beginning of the photoperiod, reached a maximum in the 6th to 7th hour and decreased during the latter part of the photoperiod. in capsicum frutescens this was apparently a reflection of t ...197316658402
efficiency and regulation of water transport in some woody and herbaceous species.the efficiency with which plants transport water is related to the water potential differences required to drive water fluxes from the soil to the leaf. a comparative study of two woody and three herbaceous species (citrus sinensis l. cv. koethen, pyrus kawakami l., helianthus annuus l. cv. mammoth russian, capsicum frutescens l. cv. yolo wonder, and sesamum indicum l. cv. glauca) indicated contrasts in water transport efficiency. depression of leaf water potential in response to transpiration i ...197416658853
four biotypes of tylenchulus semipenetrans in california identified, and their importance in the development of resistant citrus rootstocks.four biotypes (pathotypes) of the citrus nematode, tylenchulus semipenetrans, occurring in california, u.s.a. were differentiated on the basis of differences of infectivity on 'homosassa' sweet orange, 'troyer' citrange, 'pomeroy' and 'rubidoux' poncirus trifoliata, 'thompson seedless' grape, and 'manzanillo' olive. a method for differentiating biotypes of t. semipenetrans is described. field observations indicate that biotypes of this nematode are very stable. the importance of using highly inf ...197419319369
the effect of soil type on movement and infection rate of larvae of tylenchulus semipenetrans.most of the tylenchulus semipenetrans larvae applied on the surface of four soils in pots 14.5-cm deep moved no further downward than 6.5 crn, and remained in the upper half of the pot. the percentage of second-stage larvae that developed into adult females on 'homosassa' sweet orange in the soils were: sandy loam, 6.8% in the same soil with inoculation holes, 8.6%; loamy sand, 5.4%; coarse sand, 0.2%; and in sand-peat (2:1, v/v)mixture 0.04%. low percentage infection in coarse sand and sand-pea ...197419319368
spiroplasmas are the causal agents of citrus little-leaf disease.a spiroplasma isolated from citrus with little-leaf disease was grown in a cell-free medium and injected into leafhoppers (euscelis plebejus). injected leafhoppers, but not those fed on infected plants, transmitted the spiroplasma to white clover (trifolium repens cv. s100) and sweet orange (citrus sinensis cv. valencia). infected clover plants were severely stunted; infected sweet orange plants showed typical symptoms of citrus little-leaf disease. the spiroplasma was detected in clover and swe ...197419280788
effect of water stress on ethylene production by detached leaves of valencia orange (citrus sinensis osbeck).detached leaves of valencia oranges, citrus sinensis osbeck, emanated ethylene at markedly higher rates and contained more endogenous ethylene when placed under water stress at 55% relative humidity than when placed in water-saturated air. water stress induced defoliation from detached branches. relieving the water stress of such leaves by transfer to a mist chamber resulted in lowering of the rates of ethylene emanation to the level occurring in leaves maintained continuously in a mist chamber. ...197416658805
partial purification of a growth factor from orange juice which affects citrus tissue culture and its replacement by citric acid.orange (citrus sinensis l. osbeck) tissue cultures required a supply of orange juice to the medium for their vigorous growth. the growth-promoting activity of juice seemed to involve both cell division and cell enlargement. juice had no promotive activity in bioassays for auxins, gibberellins, and cytokinins. the growth promoting activity of juice was mostly transferred into 1-butanol upon partition at ph 2. gas chromatographic analysis of this acid 1-butanol fraction revealed large amounts of c ...197516659287
the effects of ph, ionic strength, and ethylene on the extraction of cellulase from abscission zones of citrus leaf explants.the solubility of cellulase extracted from the abscission zones of citrus leaf explants (citrus sinensis l. osbeck) in sodium phosphate buffer depends on the ph of the extracting solution and, to a lesser extent, on the ionic strength. by increasing molarity from 0.01 to 0.16, the solubility of cellulase increased from 51% to 89% at ph 6.1 and from 70% to 98% at ph 7. in all cases, residual cellulase was further extracted from the pellet by buffer containing 1 m nacl. most of the enzymic activit ...197516659196
metabolism of uronic acids in plant tissues: partial purification and properties of uronic acid oxidase from citrus leaves.a new enzyme, named uronic acid oxidase, was extracted and purified 67-fold by (nh(4))(2)so(4) fractionation and cm-sephadex column chromatography from ethylene-treated shamouti orange (citrus sinensis l. osbeck) leaves. the enzyme catalyzes the oxidation of d-galacturonic acid and d-glucuronic acid to the corresponding hexaric acids in the presence of molecular oxygen with the production of h(2)o(2). the ph optimum for the oxidation of d-galacturonic acid and d-glucuronic acid is between 7 and ...197516659133
cellulase activity, endogenous abscisic acid, and ethylene in four citrus cultivars during maturation.at maturity, the fruit of two early maturing orange cultivars, hamlin and pineapple (citrus sinensis [l.] osbeck), contained more ethylene and abscisic acid than the late maturing valencia and lamb summer (c. sinensis [l.] osbeck) cultivars. ethylene (up to 95 nl/l in internal atmosphere) and abscisic (50 mug/kg dry weight flavedo) increased most rapidly in pineapple, leading to increased cellulase activity and loosening of the fruit. fruit of the two late maturing cultivars contained less than ...197516659390
effects of oxamyl on the citrus nematode, tylenchulus semipenetrans, and on infection of sweet orange.foliar sprays of 4 microg/ml oxamyl on sweet orange trees in a greenhouse slightly depressed the number of tylenchulus semipenetrans larvae obtained from roots and soil, but similar treatments were not effective in two orchards. soil drench treatments decreased the number of citrus nematode larvae obtained from roots or soil of citrus plants grown itt a greenhouse and in orchards. exposure to 5-10 microg/ml of oxamyl in water was lethal to only a few second-stage larvae treated 10 days, and many ...197619308209
ethylene-induced formation of aba in citrus peel as related to chloroplast transformations.when mature green harvested shamouti oranges (citrus sinensis l. osbeck) were exposed to 35 mul/liter of ethylene, a 3-fold increase in free abscisic acid (aba) of the flavedo could be detected after 12 hours and a 10-fold increase after 24 hours, while chlorophyll destruction did not exceed 20%. the increase in free aba continued up to 24 hours and leveled off. bound aba accumulated strongly after 24 hours suggesting that excess of free aba was being converted into the bound form. similar incre ...197616659682
involvement of endogenous ethylene in the induction of color change in shamouti oranges.reducing the level of endogenous ethylene in detached shamouti oranges (citrus sinensis l. osbeck) by means of subatmospheric pressure did not alter the rate of chlorophyll destruction and color changes during the first 8 days after harvest in the presence or absence of exogenous ethylene. reducing the activity of ethylene by means of co(2)-known to be a competitive inhibitor for ethylene-inhibited chlorophyll destruction and color change in shamouti oranges ventilated with ethylene, but had no ...197616659580
pectin-polygalacturonase in citrus.extracts from citrus [citrus sinensis (l.) osbeck] pedicles rated as containing polygalacturonase and cellulase gave activities both as measured with carboxymethylcellulose and with sodium polypectate. gel electrophrosis of the extract demonstrated two region areas that contained cellulytic activity.197624430758
endogenous abscisic acid in relation to bud growth in alternate bearing ;valencia' orange.an investigation was conducted into the relation of aba (cis-trans-abscisic acid) in the dormant buds of alternate bearing ;valencia' orange (citrus sinensis [l.] osbeck) trees. aba did not appear to be related to alternate bearing but t-aba (2-transabscisic acid) did. there was 5- to 10-fold more t-aba than aba in the buds. there was more t-aba in the buds of the "on" trees than in the buds of the "off" trees, and a drastic drop in t-aba in both types of buds as spring growth approached. bud do ...197616659743
differential counteraction of ethylene effects by gibberellin a(3) and n(6)-benzyladenine in senescing citrus peel.treatment of mature citrus fruit (citrus sinensis) with ethylene induced rapid chlorophyll destruction, a rise in respiration, a release of free amino acids, an accumulation of reducing sugars, and an appearance of phenylalanine ammonia lyase activity. gibberellin a(3) (ga(3)) and n(6)-benzyladenine (ba) opposed the effects of ethylene on chlorophyll, amino acids, and to a lesser extent, reducing sugar levels. the ethylene-induced respiratory rise was only slightly modified by ga(3) and ba. phen ...197716659815
differential effects of sucrose, abscisic acid, and benzyladenine on shoot growth and callus formation in the abscission zone of excised citrus buds.the omission of sucrose from the basal medium stimulated callus formation in bud explants of citrus sinensis (l.) osbeck. moreover, it increased the abscisic acid-induced callus proliferation reported earlier in the presence of 5% sucrose (altman and goren, physiol. plant. 32: 55, 1974). the inhibition of callus formation by the addition of sucrose was not due to the high osmotic potential of the medium. benzyladenine induced callus formation slightly, in all sucrose concentrations up to 5%. the ...197716660013
dislodgeable residues of ethion in florida citrus and relationships to weather variables.five different treatments of ethion on valencia orange trees were compared for decay rates and for ethion monoxon and ethion dioxon production under different environmental conditions. the oxon metabolite levels observed were low and always below the residue level of ethion itself. there were no significant differences in the decay rates of ethion between treatments. a model of ethion decay utilizing environmental variables as a time base is presented. this model explains 94% of the variation ob ...1977901006
abscission of citrus leaf explants: no correlation with naphthaleneacetic acid conjugation in the abscission zone.the role of alpha-naphthaleneacetic acid (naa) in the control of abscission in citrus (citrus sinensis l. osbeck) leaf explants and its conjugation were studied in non-aged and 24-hour-aged explants. dipping non-aged explants in 1.5 micromolar naa for 15 minutes immediately after excision did not delay abscission whereas 150 micromolar naa effectively delayed it. as incubation time was prolonged up to 24 hours after excision, the delaying effect of both concentrations gradually increased. in gen ...197816660505
citrus tissue culture: stimulation of fruit explant cultures with orange juice.in vitro growth of explant (juice vesicle or albedo tissues) cultures from citron (citrus medica), lemon (c. limon), grapefruit (c. paradisi), sweet orange (c. sinensis), and mandarin (c. reticulata) fruits was stimulated by addition of orange juice (10% v/v optimum) to a basal medium containing murashige and skoog salts, 50 grams per liter sucrose, 100 milligrams per liter myo-inositol, 5 milligrams per liter thiamine.hcl, 2 milligrams per liter 2,4-dichlorophenoxyacetic acid and 0.5 milligrams ...197816660631
a comparative study of antigens of aspergillus fumigatus isolates from patients and soil of ornamental plants in the immunodiffusion test.the strikingly frequent and constant presence of aspergillus fimigatus in the soil of potted ornamental plants kept in private houses and hospitals has been the reason for studying the antigens of the strains found from the diagnostic and epidemiological angles. culture-filtrate antigens of a. fumigatus strains isolated from the soil of 4 different ornamental plants, epiphyllum (epiphyllum truncatum), orange tree (citrus sinensis), alpine rose (azalea indica) and christmas flower (euphorbia pulc ...197883753
accumulation of free proline in citrus leaves during cold hardening of young trees in controlled temperature regimes.free proline increased in leaves of orange (citrus sinensis [l.] osb. cv. valencia) and grapefruit (citrus paradisi macfad. cv. star ruby) trees on a wide range of citrus rootstocks during cold hardening. increases in sugars accompanied proline accumulation. during cold hardening, the rate of proline accumulation was greater in old than in young leaves. in leaves of grapefruit trees kept in the dark during cold hardening, neither proline nor sugars increased and the degree of cold hardiness was ...197916660980
role of ethylene in abscisic acid-induced callus formation in citrus bud cultures.the induction of callus formation in cultured buds of shamouti orange (citrus sinensis [l.] osbeck) by abscisic acid (aba) is a multiphasic process. (altman, and goren 1974 physiol plant 32: 55.) a study of the mediation by ethylene on this effect of aba was undertaken. it was found that: (a) ethylene and (2-chloroethyl) phosphonic acid, as well as aba, induced callus formation; (b) callus induction is best attained when explants are exposed to ethylene during the 1st day after excision; and (c) ...197916660713
inhibition of polar indole-3-acetic acid transport by cycloheximide.cycloheximide inhibits polar indoleacetic acid transport in midrib tissues of leaves of citrus (citrus sinensis [l.] osbeck) and poplar (populus deltoides bartr.) as measured by the donor-receiver agar cylinder technique. it appears that the mechanism of auxin transport inhibition by cycloheximide consists in arresting protein synthesis and not in the disruption of energy flow. the interpretation of the data takes into account the involvement of either a carrier protein or auxin-induced proton e ...197916660887
acephate and methamidophos residue behavior in florida citrus, 1976.the half-life of acephate and its hydrolysate, methamidophos, in the rind of temple and valencia oranges, and grapefruit, lemons, and tangerines was 10.3 days and 10.5 days, respectively. half-lives of acephate and methamidophos in citrus pulp were 15.0 days and 6.1 days, respectively based on 7-, 14-, and 21-day data. seven days after treatment, acephate and methamidophos reached maximum levels in rind and pulp. acephate residue levels in rind were less than 3.0 ppm 14 days after treatment; ace ...1979461112
nutrients in fresh peeled oranges and grapefruit from california and arizona.naval oranges, valencia oranges, and grapefruit, representative of the california and arizona citrus crop, were collected over three consecutive growing seasons, and the nutritional composition of their juices was determined. measured as per cent of the u.s. rdas, folic acid, magnesium, vitamin a (oranges only), vitamin b6, thiamin, and ascorbic acid are all supplied in greater proportion than the calories present, while niacin and calcium are supplied in approximately proportionate amounts.19807430512
citrus tissue culture : auxins in relation to abscission in excised pistils.an in vitro bioassay for chemicals that affect citrus abscission was used to identify three inhibitors of stylar abscission in lemon pistil explants incubated on defined nutrient media. the three inhibitors (picloram, 4-chlorophenoxyacetic acid, and 3,5,6-trichloropyridine-2-oxyacetic acid) are all auxins, and the most potent of them (i.e. picloram) was found to be at least 10 times more active in the bioassay than 2,4-dichlorophenoxyacetic acid. picloram (2 micromolar) also was shown to be effe ...198116661819
characterization of bound phenthoate residues in citrus.the metabolism and fate of phenyl ring-labeled 14c-phenthoate (0,0-dimethyl s-[alpha-(carboethoxy)benzyl] phosphorodithioate) was examined in the valencia orange fruit with emphasis on the characterization of bound phenthoate residues in the fruit peel. the products recovered from the citrus fruit wash were unchanged phenthoate, phenthoate oxon, demethyl phenthoate, phenthoate acid, ethyl mandelate, and mandelic acid. the same products, with the exception of phenthoate oxon, were found in the ac ...19817259311
laboratory evaluation of fungicides for the control of trichothecium roseum link. a new fruit rot pathogen of sweet orange. 19817198107
effect of ethylene on [c]indole-3-acetic acid metabolism in leaf tissues of woody plants.the effect of ethylene on [(14)c]indole-3-acetic acid (iaa) metabolism was investigated in defoliation sensitive leaf tissues of citrus (citrus sinensis) and resistant leaf tissues of eucalyptus (eucalyptus camaldulensis). iaa metabolites were fractionated into 80% ethanol-soluble, h(2)o-soluble, naoh-soluble, and insoluble components. in citrus, pretreatment with 25 microliters per liter ethylene for 24 hours significantly increased the amount of ethanol- and h(2)o-extractable conjugates during ...198216662665
stimulation of ethylene production in citrus leaf discs by mannitol.wound ethylene formation induced in leaf tissue of citrus (citrus sinensis [l.] osbeck cv. "washington navel") by excision was significantly stimulated by mannitol after a lag period of about 6 hours. the extent of stimulation was dependent upon the concentration of mannitol (10 to 100 millimolar). this increased ethylene production was not simply due to osmotic effect or water stress as other osmoticums tested failed to exert such an effect. the stimulatory effect of mannitol resulted from both ...198216662435
effects of exogenous ethylene on ethylene production in citrus leaf tissue.exogenous ethylene stimulated ethylene production in intact citrus (citrus sinensis l. osbeck cv. "washington navel") leaves and leaf discs following a 24-hour exposure. studies with leaf discs showed that ethylene production decreased when ethylene was removed by aeration. the extent of stimulation was dependent upon the concentration of exogenous ethylene (1-10 microliters per liter). silver ion blocked the autocatalytic effect of ethylene at concentrations of 0.5 millimolar and lower, but inc ...198216662434
inhibition of ethylene biosynthesis by aminoethoxyvinylglycine and by polyamines shunts label from 3,4-[c]methionine into spermidine in aged orange peel discs.the flux of radioactivity from 3,4-[(14)c]methionine into s-adenosyl-l-methionine (sam), 1-aminocyclopropane-1-carboxylic acid (acc), spermine, and spermidine while inhibiting conversion of acc to ethylene by 100 millimolar phosphate and 2 millimolar co(2+) was studied in aged peel discs of orange (citrus sinensis l. osbeck) fruit. inhibition up to 80% of ethylene production by phosphate and cobalt was accompanied by a 3.3 times increase of label in acc while the radioactivity in sam was only sl ...198216662214
protein scans of cold-hardened and freeze-injured "valencia" orange leaves. 19827151461
colorimetric method for field-screening above-tolerance parathion residues on and in citrus fruits.a colorimetric technique has been developed which is suitable for use as a field-screening method for detecting above-tolerance levels of parathion on and in citrus fruits. by using this method, a grower should be able to postpone harvesting a crop until parathion residues are below tolerance level, so that the crop is safe to market. the method depends on the reaction of parathion with 4-(p-nitrobenzyl)-pyridine. parathion is extracted by mixing chopped citrus rind with acetone in a hand-operat ...19836826495
aspects of salt tolerance in a nacl-selected stable cell line of citrus sinensis.a nacl-tolerant cell line which was selected from ovular callus of ;shamouti' orange (citrus sinensis l. osbeck) proved to be a true cell line variant. this conclusion is based on the following observations. (a) cells which have been removed from the selection pressure for at least four passages retain the same nacl tolerance as do cells which are kept constantly on 0.2 molar nacl. (b) na(+) and cl(-) uptake are considerably lower in salt-tolerant cells (r-10) than in salt-sensitive cells (l-5) ...198316663067
chlorophyllase activity in chlorophyll-free citrus chromoplasts.chromoplast fractions from mature, chlorophyll-less 'valencia' orange (citrus sinensis l. osbeck) flavedo (= the outer coloured layer of citrus peel) showed considerable chlorophyllase activity. acetone powders prepared from chromoplast fractions had 2.5× higher specific activity than those prepared from whole flavedo. exposure of mature, chlorophyll-less fruit to ethylene caused a 2.5 to 4.0 fold increase in chlorophyllase activity. juice chromoplasts showed negligible chlorophyllase activity. ...198324257977
cellulolytic enzymes associated with the fruit rots of citrus sinensis caused by aspergillus aculeatus and botryodiplodia theobromae.botryodiplodia theobromae and aspergillus aculeatus were inoculated in carboxymethylcellulose (cmc) medium and on filter papers. hydrolysis of the cmc medium and degradation of the filter papers were observed, indicating the production of c1 and cx cellulases by the two rot pathogens. the c1 and cx enzymes were also detected in filtrates of rotted orange fruits obtained by infection with the two pathogens. the cellulases could not induce rot development on their own. however, when they were adde ...19836624142
citrus fruits. part ii. chemistry, technology, and quality evaluation. b. technology.in part ii of this review on citrus fruits, the literature on chemistry, technology, and quality evaluation are critically considered. sweet oranges, mandarin, grapefruit, lemon, and lime are generally used for processing. the literature on chemical components of citrus fruit which include sugars, polysaccharides, organic acids, nitrogenous constituents and lipids; carotenoids which contribute to color; vitamins and minerals and flavonoids; limonoids, some of which impart bitterness to the juice ...19836380950
citrus fruits--varieties, chemistry, technology, and quality evaluation. part ii. chemistry, technology, and quality evaluation. a. chemistry.in part 2 of this review on citrus fruits, the literature on chemistry, technology, and quality evaluation are critically considered. sweet oranges, mandarin, grapefruit, lemon, and lime are generally used for processing. the literature on chemical components of citrus fruit which include sugars, polysaccharides, oraganic acids, nitrogenous constituents and lipids; carotenoids which contribute to color; vitamins and minerals, and flavonoids; limonoids, some of which impart bitterness to the juic ...19836354594
phototoxic and contact toxic reactions of the exocarp of sweet oranges: a common cause of cheilitis?irritant skin reactions were produced within 1 h after application of the exocarp of sweet oranges or alcoholic extracts therefrom. such reactions faded within 48 h. the exocarp, or extracts thereof, induced phototoxic reactions which were strongest at 72 h after exposure. the phototoxic reactions were only induced in natural blondes and only with some oranges. the in vivo phototoxic reactions were confirmed in vitro, causing a slight but clear photo-inhibition of candida albicans. only some ora ...19836861482
sugar regulation of plastid interconversions in epicarp of citrus fruit.seasonal transformations between chloroplasts and chromoplasts, as measured by changes in chlorophyll content, in the epicarp of degreening and regreening citrus sinensis (l.) osbeck cv valencia fruit closely parallelled the accumulation and later loss of soluble sugars. at any stage of development, reversing the relative soluble sugar content in the epicarp by culturing pericarp segments on agar media with low (15 millimolar) or high (150 millimolar) sucrose concentrations reversed the directio ...198416663837
metabolism of carbosulfan in valencia orange tree leaves and fruit. 19846703784
resistance of citrus fruit to mass transport of water vapor and other gases.the resistance of oranges (citrus sinensis l. osbeck) and grapefruit (citrus paradisi macf.) to ethylene, o(2), co(2), and h(2)o mass transport was investigated anatomically with scanning electron microscope and physiologically by gas exchange measurements at steady state. the resistance of untreated fruit to water vapor is far less than to ethylene, co(2) and o(2). waxing partially or completely plugs stomatal pores and forms an intermittent cracked layer over the surface of fruit, restricting ...198516664527
somatic hybrid plants obtained by protoplast fusion between citrus sinensis and poncirus trifoliata.somatic hybrid plants of rutaceae were obtained by protoplast fusion between citrus sinensis osb. ('trovita' orange) and poncirus trifoliata. protoplasts isolated from embryogenic cells of c. sinensis and from leaves of p. trifoliata, and the culture of fusion products in the presence of high concentrations of sucrose were essential requirements for the selection of hybrids. green globular embryoids derived from protoplasts resulted in the regeneration of trifoliate plants. other morphological c ...198524247330
photosynthetic activity in the flower buds of ;valencia' orange (citrus sinensis [l.] osbeck).flower buds of ;valencia' orange (citrus sinensis [l.] osbeck) were able to fix (14)co(2) into a number of compounds in their own tissues under both light and dark conditions. the total incorporation, however, was about 4-fold higher in the light than in the dark. in the light, 50% of the total (14)c label was found in the neutral fraction (sugars), 22% in the basic fraction (amino acids), and 26% in the acid-1 fraction (organic acids). in the dark, about 95% of the (14)c label was incorporated ...198516664257
residues analysis of post-harvest imidazole fungicides in citrus fruit by hplc and glc.the determination of imazalil and prochloraz fungicide residues has been carried out by hplc with an uv detector at 204 nm and by glc with an electron capture detector (ecd). in both cases fungicide residues were extracted with hexane/acetone (90:10, v/v) after ph adjustment and purified by a liquid-liquid partitioning process. when hplc was used for prochloraz and imazalil analysis, it was necessary to eliminate the interfering substances with a further clean-up process. this was also required ...19854077370
xiphidorus amazonensis n. sp. (nematoda: longidoridae) from the brazilian amazon basin.xiphidorus amazonensis n. sp. was found in the rhizospheres of jatropha curcas, musa sp., anona muricata, cassia tora, panicum laxum, paspalum fasciculatum, aeschynomene sensitiva, saccharum officinarum, manihot esculenta, abelmoschus esculentus, tamarindus indica, mangifera indica, vigna unguiculata, zea mays, commelina sp., cyperus rotundus, fimbristylis miliacea, citrus sinensis, and eichhornia crassipes on the amazon river island of xiborena, approximately 40 km southeast of manaus, capital ...198519294098
variation in radopholus citrophilus population densities in the citrus rootstock carrizo citrange.seedlings of the hybrid citrus rootstock, carrizo citrange (citrus sinensis x poncirus trifoliata) do not uniformly limit development of the citrus burrowing nematode, radopholus citrophilus. variation in nematode population densities in roots of seedlings germinating from the same seed suggests that factors responsible for nematode incompatibility are not functional or are not inherited uniformly among progeny. seeds which produced a single seedling were more likely to produce plants which supp ...198619294135
the occurrence of abscisic acid and abscisyl-beta-d-glucopyranoside in developing and mature citrus fruit as determined by enzyme immunoassay.the contents of (+)-cis-abscisic acid (aba) and alkaline-hydrolyzable aba-conjugate(s) were analyzed by means of enzyme immunoassay in partially purified extracts of developing and mature sweet orange fruit (citrus sinensis [l.] osbeck cv washington navel). a relatively small increase in aba was observed in the fruit exocarp during the natural color transition from green to orange. at the same time, the aba-conjugate level increased approximately 12-fold in this tissue. the contents of aba and a ...198616665032
presence and identification of polyamines in xylem and phloem exudates of plants.polyamines were identified by high performance liquid chromatography (benzoylation) and by thin layer chromatography (dansylation) in xylem exudates from stems of sunflowers (helianthus annuus [l.]), mung bean (vigna radiata [l.] wilczek), grapevine (vitis vinifera [l.] cv grenache), and orange (citrus sinensis [l.] osbeck, cv valencia), as well as in phloem sap (using elution into edta) of sunflower and mung bean plants. putrescine was the major polyamine detected, ranging in concentrations of ...198616665153
levels of free and conjugated abscisic acid in developing floral organs of the navel orange (citrus sinensi [l.] osbeck cv washington).the levels of abscisic acid (aba) and alkaline-hydrolyzable aba-conjugate (putatively identified as the glucosyl ester, abscisyl-beta-d-glucopyranoside) were determined by enzyme immunoassay in the organs of developing navel orange (citrus sinensis [l.] osbeck cv washington) flowers. although both compounds were detected in every tissue, developmentally related differences between organs in the total and relative contents were observed. the highest aba levels were observed in the stigma/style sh ...198616665156
accumulation of chlorophyll, chloroplastic proteins, and thylakoid membranes during reversion of chromoplasts to chloroplasts in citrus sinensis epicarp.in vitro culture of pericarp segments from fruit of citrus sinensis (l.) osbeck cv valencia was used to determine the temporal sequence in development of chloroplasts from chromoplasts during regreening of the epicarp. regreening of chromoplasts closely resembled greening of etioplasts, except that regreening proceeded much more slowly. chlorophyll, the light-harvesting chlorophyll a/b binding protein of photosystem ii, the chlorophyll a binding protein of reaction center p-700 of photosystem i, ...198616664794
role of internal potassium in maintaining growth of cultured citrus cells on increasing nacl and cacl(2) concentrations.shamouti orange (citrus sinensis l. osbeck) salt-tolerant cells were grown under low water potential conditions induced by polyethylene glycol (peg), nacl, and cacl(2). on the basis of equal osmotic potentials, peg was the least inhibitory, nacl next, and cacl(2) the most inhibitory. the relation between growth capacity and ion content can be summarized as follows. (a) internal k(+) concentration was a major factor which changed in the presence of peg, nacl, and cacl(2) and probably played a key ...198716665716
uniformity of plants regenerated from orange (citrus sinensis osb.) protoplasts.using 25 plants (protoclones) regenerated from orange (citrus sinensis osb.) protoplasts, several characters, including leaf and flower morphology, leaf oil, isozyme patterns and chromosome number, were examined. no significant variations in each character were recorded among the protoclones. uniformity observed among protoclones was identical to that of nucellar seedlings.198724241449
glc analysis of thiabendazole residues in citrus fruit.analysis of thiabendazole (tbz) residues in citrus fruit is performed using a bonded phase, se-54, fused-silica capillary column. the fungicide is extracted with hexane: ethyl acetate (90:10, v/v) at high ph and, after a short cleanup, determined by gas-liquid chromatography with a nitrogen-phosphorus detector (npd). recoveries through the method are always higher than 80% and the limit of detection is 0.01 mg/kg. tbz residues are determined in whole fruit, peel, and pulp of "washington navel" o ...19873558699
diversity of citrus tristeza virus isolates indicated by dsrna analysis.one major dsrna of molecular weight (mw) 13.3 x 10(6) and two others (mw 1.9 x 10(6) and 0.8 x 10(6] were routinely detected by polyacrylamide gel electrophoresis in extracts from sweet orange (citrus sinensis) or citron (citrus medica) infected with each of 66 isolates of citrus tristeza virus (ctv). several additional dsrna were also commonly detected, usually as weakly stained bands in reproducible positions in gels, but some were very prominent, e.g., a dsrna of mw 1.7 x 10(6) associated wit ...19873692724
relationship between salt tolerance and resistance to polyethylene glycol-induced water stress in cultured citrus cells.salt-tolerant selected cells of shamouti orange (citrus sinensis) and sour orange (citrus aurantium) grew considerably better than nonselected cells at any nacl concentration tested up to 200 millimolar. also, the growth response of each treatment was identical in the two species. however, the performance of cells of the two species under osmotic stress induced by polyethylene glycol (peg), which is presumably a nonabsorbed osmoticum, was significantly different. the nonselected shamouti cell li ...198716665715
evaluation of polyamine and proline levels during low temperature acclimation of citrus.the polyamines (pa) putrescine (put), spermidine (spd), and spermine (spm) were measured during 3 weeks exposure to cold hardening (15.6 degrees c day and 4.4 degrees c night) and nonhardening (32.2 degrees c day and 21.1 degrees c night) temperature regimes in three citrus cultivars: sour orange (so) (citrus aurantium l.), ;valencia' (val) (citrus sinensis l. osbeck), and rough lemon (rl) (citrus jambhiri lush). the changes in pa were compared to the amount of free proline, percent wood kill an ...198716665504
oral ethanol self-administration in free-feeding rhesus monkeys.the ability of a conditioning procedure to establish oral ethanol self-administration in free-feeding rhesus monkeys was assessed. the conditioning procedure required the monkey to drink an ethanol solution in order to have access to a sweet orange-flavored solution. following an average of 14 sessions under these conditions, the orange solution was no longer delivered and ethanol solution alone was made available in the sessions. during both the conditioning and the ethanol self-administration ...19883064639
synergistic effect of fungicides on resistant strains of penicillium italicum and penicillium digitatum.in vitro and in vivo tests were carried out to study the inhibitory effect of mixtures of sodium orthophenylphenate, methyl-1-(butyl-carbamoyl)-2-benzimidazole ('benomyl') and sodium orthophenylphenate, 2-(4-thiazolil)-benzimidazole ('thiabendazole') on growth of strains of penicillium italicum and p. digitatum resistant to sodium orthophenylphenate, 'benomyl' and 'thiabendazole'. 'in vitro' tests showed a synergistic effect of the mixtures; 10 ppm of sodium orthophenylphenate and 10 ppm of the ...19883275313
carbon cost of the fungal symbiont relative to net leaf p accumulation in a split-root va mycorrhizal symbiosis.translocation of (14)c-photosynthates to mycorrhizal (+ +), half mycorrhizal (0+), and nonmycorrhizal (00) split-root systems was compared to p accumulation in leaves of the host plant. carrizo citrange seedlings (poncirus trifoliata [l.] raf. x citrus sinensis [l.] osbeck) were inoculated with the vesicular-arbuscular mycorrhizal fungus glomus intraradices schenck and smith. plants were exposed to (14) co(2) for 10 minutes and ambient air for 2 hours. three to 4% of recently labeled photosyntha ...198816665934
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