Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| a comparative study of antigens of aspergillus fumigatus isolates from patients and soil of ornamental plants in the immunodiffusion test. | the strikingly frequent and constant presence of aspergillus fimigatus in the soil of potted ornamental plants kept in private houses and hospitals has been the reason for studying the antigens of the strains found from the diagnostic and epidemiological angles. culture-filtrate antigens of a. fumigatus strains isolated from the soil of 4 different ornamental plants, epiphyllum (epiphyllum truncatum), orange tree (citrus sinensis), alpine rose (azalea indica) and christmas flower (euphorbia pulc ... | 1978 | 83753 |
| behavior of parathion residues in the florida "valencia" orange agroecosystem. | 2013 | 447929 | |
| acephate and methamidophos residue behavior in florida citrus, 1976. | the half-life of acephate and its hydrolysate, methamidophos, in the rind of temple and valencia oranges, and grapefruit, lemons, and tangerines was 10.3 days and 10.5 days, respectively. half-lives of acephate and methamidophos in citrus pulp were 15.0 days and 6.1 days, respectively based on 7-, 14-, and 21-day data. seven days after treatment, acephate and methamidophos reached maximum levels in rind and pulp. acephate residue levels in rind were less than 3.0 ppm 14 days after treatment; ace ... | 1979 | 461112 |
| dislodgeable residues of ethion in florida citrus and relationships to weather variables. | five different treatments of ethion on valencia orange trees were compared for decay rates and for ethion monoxon and ethion dioxon production under different environmental conditions. the oxon metabolite levels observed were low and always below the residue level of ethion itself. there were no significant differences in the decay rates of ethion between treatments. a model of ethion decay utilizing environmental variables as a time base is presented. this model explains 94% of the variation ob ... | 1977 | 901006 |
| structures of new cyclic peptides in young unshiu (citrus unshiu marcov.), orange (citrus sinensis osbeck.) and amanatsu (citrus natsudaidai) peelings. | four cyclic peptides were isolated from young unshiu (unripe fruit), orange and amanatsu peelings, and their structures were established on the basis of fab-ms (cid method) and 2d-nmr spectroscopic data, and by chemical evidence. they were each found to consist of seven or eight amino acids. | 1991 | 1368762 |
| quantitative fluorometric analysis of plant and microbial chitosanases. | a quantitative fluorometric assay for chitosanase activity in bacterial and plant tissues was developed. the assay can be conducted with either finely milled preparations of chitosan in suspension or dissolved chitosan; activity is based on measurements of glucosamine (glcn) or oligomers of glcn. glcn is detected fluorometrically after reaction with fluorescamine with detection in the nanomole range. fluorescence measurements of chitosanase activity and radioassay of chitinase in commercial prep ... | 1992 | 1514694 |
| citrus peel oil extracts as mosquito larvae insecticides. | susceptibility tests were carried out in culex quinquefasciatus larvae using peel oil extracts of bitter orange (citrus aurantium), orange (citrus sinensis) and lemon (citrus limon). larvae mortalities were observed indicating that the extracts may contain potentially useful insecticides. | 1992 | 1644035 |
| aldicarb residues in citrus soil, leaves and fruits. | aldicarb was applied to the soil of late valencia orange field plots. a comparative study between four analytical methods is reported, the npd-gas chromatography method being the best choice. the work also reports the study of accumulation and persistence of aldicarb and its biologically active metabolites in the soil, leaves and fruits (rind and pulp, separately). residue content, at the ppb level, was found in the order leaves much greater than rind greater than pulp. carbamate was found to ac ... | 1990 | 2262036 |
| determination of limonin in grapefruit juice and other citrus juices by high-performance liquid chromatography. | a method has been developed for the quantitation of the bitter component limonin in grapefruit juice and other citrus juices. the sample clean-up consisted of centrifugation, filtration and a selective, rapid and reproducible purification with a c2 solid-phase extraction column. the limonin concentration was determined by high-performance liquid chromatography on a c18 column with uv detection at 210 nm. a linear response was obtained from 0.0 to 45 ppm limonin. the minimum detectable amount was ... | 1989 | 2722986 |
| oral ethanol self-administration in free-feeding rhesus monkeys. | the ability of a conditioning procedure to establish oral ethanol self-administration in free-feeding rhesus monkeys was assessed. the conditioning procedure required the monkey to drink an ethanol solution in order to have access to a sweet orange-flavored solution. following an average of 14 sessions under these conditions, the orange solution was no longer delivered and ethanol solution alone was made available in the sessions. during both the conditioning and the ethanol self-administration ... | 1988 | 3064639 |
| ethion distribution in florida valencia oranges. | 1988 | 3179522 | |
| quantitative determination of limonin in citrus juices by hplc using computerized solvent optimization. | the commercially available computer program, drylab, for optimization of separations by high-performance liquid chromatography (hplc) using binary solvent mixtures is used to improve an hplc method for separation of the bitter principle, limonin, in grapefruit and navel orange juices. best conditions for separation of limonin in a reasonable time are 30 to 32% acetonitrile in water at 0.9 ml/min using a 5-micron c18 column 10 cm long. these conditions are used to analyze grapefruit and navel ora ... | 1988 | 3225313 |
| synergistic effect of fungicides on resistant strains of penicillium italicum and penicillium digitatum. | in vitro and in vivo tests were carried out to study the inhibitory effect of mixtures of sodium orthophenylphenate, methyl-1-(butyl-carbamoyl)-2-benzimidazole ('benomyl') and sodium orthophenylphenate, 2-(4-thiazolil)-benzimidazole ('thiabendazole') on growth of strains of penicillium italicum and p. digitatum resistant to sodium orthophenylphenate, 'benomyl' and 'thiabendazole'. 'in vitro' tests showed a synergistic effect of the mixtures; 10 ppm of sodium orthophenylphenate and 10 ppm of the ... | 1988 | 3275313 |
| liquid chromatographic profiles of major carotenoid esters in commercially processed california navel and valencia orange juice concentrates. | a procedure for establishing profiles of major carotenoid esters in commercially processed valencia and navel orange juice concentrates by reversed-phase liquid chromatography (lc) using sudan 1 as internal standard is described. the procedure involved conversion of 5,6-epoxides in heat concentrated citrus juices to more stable 5,8-epoxides by treatment of extracted carotenoids with hydrochloric acid followed by dual wavelength analyses at 400 and 465 nm using lc. the esters of 5,8-furanoids (au ... | 2010 | 3417817 |
| glc analysis of thiabendazole residues in citrus fruit. | analysis of thiabendazole (tbz) residues in citrus fruit is performed using a bonded phase, se-54, fused-silica capillary column. the fungicide is extracted with hexane: ethyl acetate (90:10, v/v) at high ph and, after a short cleanup, determined by gas-liquid chromatography with a nitrogen-phosphorus detector (npd). recoveries through the method are always higher than 80% and the limit of detection is 0.01 mg/kg. tbz residues are determined in whole fruit, peel, and pulp of "washington navel" o ... | 1987 | 3558699 |
| purification of phospholipase d from citrus callus tissue. | phospholipase d in extracts of soluble proteins from callus cultures derived from cotyledons of citrus sinensis (l.) osbeck is activated by ca2+ and anionic detergents and has a ph optimum of 6.5. the enzyme was purified 703-fold over the crude protein extract with a yield of 15% by ammonium sulfate precipitation, ion exchange chromatography, gel filtration, hydrophobic interaction chromatography, and preparative acrylamide gel electrophoresis. preparative electrophoresis was carried out using c ... | 2013 | 3688883 |
| diversity of citrus tristeza virus isolates indicated by dsrna analysis. | one major dsrna of molecular weight (mw) 13.3 x 10(6) and two others (mw 1.9 x 10(6) and 0.8 x 10(6] were routinely detected by polyacrylamide gel electrophoresis in extracts from sweet orange (citrus sinensis) or citron (citrus medica) infected with each of 66 isolates of citrus tristeza virus (ctv). several additional dsrna were also commonly detected, usually as weakly stained bands in reproducible positions in gels, but some were very prominent, e.g., a dsrna of mw 1.7 x 10(6) associated wit ... | 1987 | 3692724 |
| gas chromatographic determination of fenpropimorph residues in citrus fruit. | a rapid gas chromatographic method for determining fenpropimorph residues in citrus fruit is reported. the fungicide is extracted with hexane after ph adjustment of the fruit homogenate. a short liquid-liquid partitioning process is performed before gas chromatography on an ov-17 column with nitrogen-phosphorus specific detection. the limit of detection of the method was 0.01 mg/kg, based on a 25 g sample. recovery was always higher than 70%. fenpropimorph residues in "washington navel" oranges ... | 2016 | 3771458 |
| residues analysis of post-harvest imidazole fungicides in citrus fruit by hplc and glc. | the determination of imazalil and prochloraz fungicide residues has been carried out by hplc with an uv detector at 204 nm and by glc with an electron capture detector (ecd). in both cases fungicide residues were extracted with hexane/acetone (90:10, v/v) after ph adjustment and purified by a liquid-liquid partitioning process. when hplc was used for prochloraz and imazalil analysis, it was necessary to eliminate the interfering substances with a further clean-up process. this was also required ... | 1985 | 4077370 |
| persistence of omite residues on and in navel oranges and lemons and in laboratory-processed citrus pulp cattle feed. | 1971 | 4332483 | |
| inhibition of murine subcutaneous and intravenous benzo(rst)pentaphene. carcinogenesis by sweet orange oils and d-limonene. | 1971 | 5090417 | |
| distribution of radiocarbon in valencia oranges after treatment with 14 c-cycloheximide. | 2013 | 5132643 | |
| persistence of azodrin residues on and in valencia oranges and in laboratory-processed citrus pulp cattle feed. | 1970 | 5474246 | |
| peel oils of different types of citrus sinensis l. and c. aurantium l. growing in egypt. | 1971 | 5572114 | |
| constituents of valencia oranges. | 1969 | 5795080 | |
| subcellular distribution of fluoride in navel orange leaves. | 1965 | 5850916 | |
| site of fluoride accumulation in navel orange leaves. | fluoride-polluted navel orange leaves, citrus sinensis (linn.) osbeck, were fractionated into the subcellular components in hexane/carbon tetrachloride mixtures having various densities. fluoride was determined at each fraction. analyses were also made for the subcellular distribution of chlorophyll, nitrogen, and dna to assess the extent of cross-contamination of each component. the fraction containing cell wall, nuclei, and partly broken cells apparently contained a major amount of fluoride. h ... | 1966 | 5908632 |
| citrus fruits--varieties, chemistry, technology, and quality evaluation. part ii. chemistry, technology, and quality evaluation. a. chemistry. | in part 2 of this review on citrus fruits, the literature on chemistry, technology, and quality evaluation are critically considered. sweet oranges, mandarin, grapefruit, lemon, and lime are generally used for processing. the literature on chemical components of citrus fruit which include sugars, polysaccharides, oraganic acids, nitrogenous constituents and lipids; carotenoids which contribute to color; vitamins and minerals, and flavonoids; limonoids, some of which impart bitterness to the juic ... | 1983 | 6354594 |
| citrus fruits. part ii. chemistry, technology, and quality evaluation. b. technology. | in part ii of this review on citrus fruits, the literature on chemistry, technology, and quality evaluation are critically considered. sweet oranges, mandarin, grapefruit, lemon, and lime are generally used for processing. the literature on chemical components of citrus fruit which include sugars, polysaccharides, organic acids, nitrogenous constituents and lipids; carotenoids which contribute to color; vitamins and minerals and flavonoids; limonoids, some of which impart bitterness to the juice ... | 1983 | 6380950 |
| cellulolytic enzymes associated with the fruit rots of citrus sinensis caused by aspergillus aculeatus and botryodiplodia theobromae. | botryodiplodia theobromae and aspergillus aculeatus were inoculated in carboxymethylcellulose (cmc) medium and on filter papers. hydrolysis of the cmc medium and degradation of the filter papers were observed, indicating the production of c1 and cx cellulases by the two rot pathogens. the c1 and cx enzymes were also detected in filtrates of rotted orange fruits obtained by infection with the two pathogens. the cellulases could not induce rot development on their own. however, when they were adde ... | 1983 | 6624142 |
| metabolism of carbosulfan in valencia orange tree leaves and fruit. | 1984 | 6703784 | |
| colorimetric method for field-screening above-tolerance parathion residues on and in citrus fruits. | a colorimetric technique has been developed which is suitable for use as a field-screening method for detecting above-tolerance levels of parathion on and in citrus fruits. by using this method, a grower should be able to postpone harvesting a crop until parathion residues are below tolerance level, so that the crop is safe to market. the method depends on the reaction of parathion with 4-(p-nitrobenzyl)-pyridine. parathion is extracted by mixing chopped citrus rind with acetone in a hand-operat ... | 1983 | 6826495 |
| phototoxic and contact toxic reactions of the exocarp of sweet oranges: a common cause of cheilitis? | irritant skin reactions were produced within 1 h after application of the exocarp of sweet oranges or alcoholic extracts therefrom. such reactions faded within 48 h. the exocarp, or extracts thereof, induced phototoxic reactions which were strongest at 72 h after exposure. the phototoxic reactions were only induced in natural blondes and only with some oranges. the in vivo phototoxic reactions were confirmed in vitro, causing a slight but clear photo-inhibition of candida albicans. only some ora ... | 1983 | 6861482 |
| protein scans of cold-hardened and freeze-injured "valencia" orange leaves. | 1982 | 7151461 | |
| laboratory evaluation of fungicides for the control of trichothecium roseum link. a new fruit rot pathogen of sweet orange. | 1981 | 7198107 | |
| characterization of bound phenthoate residues in citrus. | the metabolism and fate of phenyl ring-labeled 14c-phenthoate (0,0-dimethyl s-[alpha-(carboethoxy)benzyl] phosphorodithioate) was examined in the valencia orange fruit with emphasis on the characterization of bound phenthoate residues in the fruit peel. the products recovered from the citrus fruit wash were unchanged phenthoate, phenthoate oxon, demethyl phenthoate, phenthoate acid, ethyl mandelate, and mandelic acid. the same products, with the exception of phenthoate oxon, were found in the ac ... | 1981 | 7259311 |
| nutrients in fresh peeled oranges and grapefruit from california and arizona. | naval oranges, valencia oranges, and grapefruit, representative of the california and arizona citrus crop, were collected over three consecutive growing seasons, and the nutritional composition of their juices was determined. measured as per cent of the u.s. rdas, folic acid, magnesium, vitamin a (oranges only), vitamin b6, thiamin, and ascorbic acid are all supplied in greater proportion than the calories present, while niacin and calcium are supplied in approximately proportionate amounts. | 1980 | 7430512 |
| a putative vacuolar processing protease is regulated by ethylene and also during fruit ripening in citrus fruit. | a putative citrus vacuolar processing thiolprotease cdna (cit-vac) was isolated from a cdna library of citrus fruits (citrus sinensis l. osbeck var washington navel). the cdna is 58 and 57% identical with vacuolar processing seed proteases from castor bean and soybean, respectively. the citvac sequence shows a typical signal peptide for entering into the endoplasmic reticulum and two glycosylation signals. using an in vitro transcription-translation system, we show that the citvac precursor is a ... | 1995 | 7480346 |
| preparation of high molecular weight genomic dna from nuclei of woody plants. | we have developed a rapid and reliable method for preparation of high molecular weight genomic dna from sweet orange (citrus sinensis) suitable for subsequent digestion by rarely cutting restriction enzymes and then separation by pulsed-field gel electrophoresis (pfge). methods previously described for preparation of plant dna prior to pfge involved protoplast isolation, a procedure that can be inefficient and time-consuming for several plant species. nuclei isolated from plant tissues were embe ... | 1995 | 7495551 |
| [a preliminary study on bioactivity of orange and tangerine peel extracts against aphis and mites]. | an assay was made on the bioactivity of the extracts of tangerine peel from cinocitrus tangerina, orange peel from citrus sinensis and mixed tangerine peel from cinocitrus sp. against aphis semia phis heraclei, aphis craccivora, uroleucon gobonis and myzus persicae using residual film or topical method, and against mites tetranychus viennensis and t. trancatus using slide-dip or immersion method. test results show that these extracts have strong bioactivity against aphids and mites. the correcte ... | 1995 | 7576134 |
| evidence for the involvement of ethylene in the expression of specific rnas during maturation of the orange, a non-climacteric fruit. | twelve cdnas corresponding to mrnas inducible by ethylene were isolated by differential screening of a cdna library from ethylene-treated citrus sinensis fruits. northern analysis of rna extracted from flavedo of ethylene-treated fruits and from fruits at different maturation stages showed that some of the mrnas corresponding to these cdnas were regulated both by ethylene treatment and during fruit maturation. the effect of exogenous ethylene on leaves and of endogenous ethylene on flowers showe ... | 1995 | 7579188 |
| dissipation of herbicide residues in the soil of a citrus orchard (citrus sinensis l. osbeck) after the ninth consecutive annual application. | 1995 | 7579939 | |
| characterization of cdna clones for differentially expressed genes in embryos of dormant and nondormant avena fatua l. caryopses. | the molecular regulation of seed dormancy was investigated using differential display to visualize and isolate cdnas representing differentially expressed genes during early imbibition of dormant and nondormant avena fatua l. embryos. of about 3000 cdna bands examined, 5 cdnas hybridized with mrnas exhibiting dormancy-associated expression patterns during the first 48 h of inhibition, while many more nondormancy-associated cdnas were observed. dormancy-associated clone afd1 hybridized with a 1.5 ... | 1995 | 7787176 |
| identification and characterization of acidic hydrolases with chitinase and chitosanase activities from sweet orange callus tissue. | acidic chitinases (ec 3.2.1.14) were isolated and characterized from 4-week-old nonembryogenic citrus sinensis l. osbeck cv 'valencia' callus tissue. the enzymes were purified using size exclusion, anion exchange, and chromatofocusing hplc techniques. eleven isoforms were isolated with m(r)s between 26,000 and 37,400. eight of the isoforms were purified to homogeneity, and all but one cross-reacted with a polyclonal antibody raised against a basic class i potato leaf chitinase. the isoelectric p ... | 1994 | 7952962 |
| the effect of home processing on postharvest fungicide residues in citrus fruit: residues of imazalil, 2-phenylphenol and thiabendazole in 'home-made' marmalade, prepared from late valencia oranges. | the effects of domestic marmalade-making procedures on residues of imazalil, 2-phenylphenol and thiabendazole in 'sweet' oranges were investigated by comparing residue levels in raw fruit and in marmalades, prepared by heating the oranges with water and sugar for about 4 h in a preserving pan on a gas ring or for about 1 h in a microwave oven. determination of residues in raw oranges and in marmalades was carried out by extraction with dichloromethane, followed by clean-up and reversed phase hpl ... | 2013 | 8181633 |
| interspecific somatic hybrid of ichang papeda with valencia orange. | protoplasts from the leaf of ichang papeda (citrus ichangensis swingle) were fused with the protoplasts of embryogenic suspension culture of valencia orange (citrus sinensis osbeck) in vitro by polyethylene glycol (peg)-induced fusion. the regenerated embryoids were malformed and were transferred onto shoot induction medium. the shoots were then grafted on 15-day-old seedlings of trifoliate orange in vitro. chromosome counts of the young leaves showed that the parents were diploids, 2n = 2x = 18 ... | 1993 | 8199318 |
| isolates of citrus exocortis viroid recovered by host and tissue selection. | isolates of citrus exocortis viroid (cev) from a single sweet orange citrus source have been selected by sequential passage through the alternative hosts citron, gynura aurantiaca, a hybrid tomato lycopersicon esculentum x l. peruvianum, and from disorganized callus culture of the hybrid tomato. the distinctions in symptom expression, titre and electrophoretic mobility among the cev isolates, operationally termed cevc (citron), cevg (gynura), cevt (tomato) and cevcls (callus) are supported by ch ... | 1993 | 8245858 |
| an antifungal compound produced by grapefruit and valencia orange after wounding of the peel. | wounding citrus induces the production of the antifungal compound 1, isolated from peel of injured grapefruit, citrus paradisa, and injured orange, citrus sinensis. interpretation of ms, uv, and 1d and 2d nmr spectroscopic data led to the identification of the new compound as 3-[4-hydroxy,3-(3-methyl-2-butenyl)-phenyl]-2-(e)-propenal. | 1993 | 8254356 |
| nucleotide sequence of the coat protein gene of citrus tristeza virus: comparison of biologically diverse isolates collected in israel. | the sequences of the coat protein genes of four seedling yellows (sy) and four non-sy (nsy) of citrus tristeza virus (ctv) isolates, which were collected in israel over a period of 30 years, were analyzed. pairwise comparisons showed extensive similarities in the nucleotide and amino acid sequences of six isolates designated the vt group. this group consists of three nsy isolates that cause a very mild ctv reaction on the sensitive combination of sweet orange (swo) grafted on sour orange (so), a ... | 1993 | 8279125 |
| production and characterization of polyclonal antibodies in rabbits to 4s-limonene synthase from spearmint (mentha spicata). | limonene synthase, a monoterpene cyclase from the oil glands of spearmint (mentha spicata) leaves that catalyzes the conversion of geranyl pyrophosphate to (-)-4s-limonene, was purified, and polyclonal antibodies were generated in rabbits against the sodium dodecyl sulfate-denatured protein. immunoblotting analysis revealed that the antibodies were very specific for denatured limonene synthase from all mentha species tested. however, no immunological cross-reactivity was observed with denatured ... | 1993 | 8442666 |
| molecular characterization of salt-stress-associated protein in citrus: protein and cdna sequence homology to mammalian glutathione peroxidases. | a gene encoding for a citrus salt-stress-associated protein (cit-sap) was cloned from citrus sinensis salt-treated cell suspension. the gene, designated csa, was isolated from a cdna expression library. the partial amino acid sequence of the protein, as well as that deduced from the nucleotide sequence of csa, revealed a considerable homology to mammalian glutathione peroxidase (gp), and to clone 6p229 from tobacco protoplasts. the increased expression of cit-sap in nacl-treated cultured citrus ... | 1993 | 8467085 |
| multiple species of defective rnas in plants infected with citrus tristeza virus. | alemow (citrus macrophylla) and sweet orange (c. sinensis) plants infected, respectively, with several israeli and florida isolates of the citrus tristeza virus (ctv) were found to contain multiple species of rna molecules with features similar to defective-interfering rnas. northern blot hybridizations of dsrnas extracted from serial passages of the israeli vt isolate (ctv-vt) and from different plants infected with a single source of inoculum showed considerable variation both in the presence ... | 1995 | 8525626 |
| analysis of bitter essential oils from orange and grapefruit by high-performance liquid chromatography with microbore columns. | the analysis of bitter orange and grapefruit essential oils (non-volatile fraction) was carried out by hplc in normal- and reversed-phase mode with uv detection. these oils were compared with the sweet orange and mandarin essential oils, analyzed previously. for the identification of chromatographic peaks, fractionation by rp-hplc was carried out. the purified fractions were analyzed by gc-ms and lc-ms. some new compounds were found, together with many others already identified in different citr ... | 1996 | 8680599 |
| polyembryony in citrus. accumulation of seed storage proteins in seeds and in embryos cultured in vitro. | citrus exhibits polyembryonic seed development, an apomictic process in which many maternally derived embryos arise from the nucellus surrounding the developing zygotic embryo. citrus seed storage proteins were used as markers to compare embryogenesis in developing seeds and somatic embryogenesis in vitro. the salt-soluble, globulin protein fraction (designated citrin) was purified from citrus sinensis cv valencia seeds. citrins separated into two subunits averaging 22 and 33 kd under denaturing ... | 1996 | 8742336 |
| purification of a zn-binding phloem protein with sequence identity to chitin-binding proteins. | in citrus blight, a decline disorder of unknown etiology, the tree canopy exhibits symptoms of zn deficiency while zn accumulates in the trunk phloem. we have purified a zn-binding protein (zbp) from phloem tissue of healthy and blight-affected citrus (citrus sinensis [l.] osbeck on citrus jambhiri [l.]). the molecular weight of the zbp was estimated to be 5000 by size-exclusion chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. ion-exchange chromatography at ph 8.0 de ... | 1996 | 8742339 |
| characterization of heterochromatic regions in 'trovita' orange (citrus sinensis osbeck) chromosomes by the fluorescent staining and fish methods. | cma/dapi double staining and fish methods using rdna and telomere specific repeated sequences as probes were applied to characterize the large amount of heterochromatin in the telomeric regions of 'trovita' orange (citrus sinensis osbeck) chromosomes. as a result, all the heterochromatic regions were cma+/dapi-, and thus were gc rich. three cma+/dapi- heterochromatic regions were positive to the 26s rdna probe. telomere arrays consisting of tm(a)gn were detected on the extreme ends of each chrom ... | 1996 | 8890521 |
| in vitro cytotoxic effect of some medicinal plants containing flavonoids. | aqueous, ethanolic and petroleum ether extracts of citrus sinensis l. (osbeck), euphrasia officinalis l., glycyrrhiza glabra l., matricaria recutita l., rosa canina l. and ruta graveolens l. have been studied. the cytotoxicity of the drugs assayed was evaluated "in vitro" by means of the dye test using cells of the yoshida ascites sarcoma. the aqueous extract of citrus sinensis l. (osbeck); the petroleum ether extract of glycyrrhiza glabra l.; the ethanolic and petroleum ether extracts of rosa c ... | 1996 | 8913055 |
| characterization of seven basic endochitinases isolated from cell cultures of citrus sinensis (l.). | seven endochitinases (ec 3.2.1.14) (relative molecular masses 23,000-28,000 and isoelectric points 10.3-10.4) were purified from nonembryogenic citrus sinensis l. osbeck cv. valencia callus tissue. the basic chitinase/lysozyme from this tissue (bclvc) exhibited lysozyme, chitinase and chitosanase activities and was determined to be a class iii chitinase. while bclvc acted as a lysozyme at ph 4.5 and low ionic strength (0.03) it acted as a chitinase/chitosanase at high ionic strengths (0.2) with ... | 1996 | 8931350 |
| cdna cloning and expression of a class ii acidic chitinase from sweet orange. | a citrus cdna encoding a class ii acidic chitinase was isolated from a nonembryogenic cell line of sweet orange using the tobacco cdna clone prob3. northern blot analysis revealed that the corresponding mrna is expressed in young, green bark but not in leaves, roots, or flavedo. | 1997 | 9116036 |
| chemical composition and organoleptic evaluation of juice from steamed cashew apple blended with orange juice. | fully riped cashew apples (yellow variety) were steamed for 7 minutes prior to juice extraction. the extracted juice was blended with various proportions of sweet orange juice. chemical composition and organoleptic evaluation were carried out on both the blended and unblended juices. the ascorbic acid content of unsteamed cashew apple juice was 287 mg/100 ml. steaming of the cashew apple prior to juice extraction resulted in a decreased (230 mg/100 ml) content of ascorbic acid. it also led to sl ... | 1997 | 9477424 |
| the chromosomes of citrus and poncirus species and hybrids: identification of characteristic chromosomes and physical mapping of rdna loci using in situ hybridization and fluorochrome banding. | in situ hybridization of 18s-5.8s-25s rdna probes labeled with biotin or rhodamine and 5s rdna probes labeled with digoxigenin was used to locate rdna sites on root-tip metaphase chromosomes of citrus sinensis l. (2n = 2x = 18), poncirus trifoliata l. raf. (2n = 2x = 18), and citrus x poncirus hybrids (2n = 2x = 18). counterstaining with the fluorochromes chromomycin a3 and dapi uniquely identified many but not all chromosomes. c. sinensis had five 18s-25s rdna sites, p. trifoliata had seven, an ... | 2000 | 9487679 |
| occurrence of citrus viroids in costa rica. | a survey for citrus viroids was conducted in the major citrus commercial growing areas in costa rica. screening of 36 sweet orange and 12 lemon trees resulted in the detection of members of four of the five citrus viroid groups as determined by nucleic acid hybridization using specific rna probes and polymerase chain reaction (pcr) using specific oligonucleotide primers. cevd, cvd-iia, cvd-iib and cvd-iii viroids were found to be widespread in the three main regions of commercial citrus producti ... | 1997 | 9611301 |
| insecticidal properties of citrus oils against culex pipiens and musca domestica. | peel oils of lemon, grapefruit and navel orange were tested for insecticidal activities against larvae and adults of culex pipiens and musca domestica. lemon peel oil was the most effective against larvae and adults of c. pipiens. grapefruit peel oil was more toxic to adults of m. domestica while lemon oil, was more toxic musca larvae. on the other hand, the orange peel oil was the least effective against larvae and adults of both species. the toxicity of oils applied to larval stages was extend ... | 1998 | 9707687 |
| relationship between volatile components of citrus fruit essential oils and antimicrobial action on penicillium digitatum and penicillium italicum. | this study examined the effect of volatile components of citrus fruit essential oils on p. digitatum and p. italicum growth. the hydrodistilled essential oils of orange (citrus sinensis cvv. "washington navel", "sanguinello", "tarocco", "moro", "valencia late", and "ovale"), bitter (sour) orange (c. aurantium), mandarin (c. deliciosa cv. "avana"), grapefruit (c. paradisi cvv. "marsh seedless" and "red blush"), citrange (c. sinensis x poncirus trifoliata cvv. "carrizo" and "troyer"), and lemon (c ... | 1998 | 9761340 |
| pectin methyl esterase from orange fruit: characterization and localization by in-situ hybridization and immunohistochemistry. | pectin methyl esterase (pme) from orange (citrus sinensis l.) fruit peels has been purified by ammonium sulphate precipitation, and ion-exchange and gel-filtration chromatography. characterization of the enzyme revealed a 36-kda protein with an isoelectric point > 9, a ph optimum at 7 and temperature optimum at 50 degrees c. the substrate specificity and kinetic experiments showed that the affinity of pme for pectin was highly dependent on the degree of esterification (de) of the pectin, with k( ... | 1998 | 9821684 |
| a triply cloned strain of xylella fastidiosa multiplies and induces symptoms of citrus variegated chlorosis in sweet orange | xylella fastidiosa isolate 8.1.b obtained from a sweet orange tree affected by citrus variegated chlorosis in the state of sao paulo, brazil, and shown in 1993 to be the causal agent of the disease, was cloned by repeated culture in liquid and on solid pw medium, yielding triply cloned strain 9a5c. the eighth and the 16th passages of strain 9a5c were mechanically inoculated into sweet orange plants. presence of x. fastidiosa in sweet orange leaves of shoots having grown after inoculation (first- ... | 1999 | 10398837 |
| enhancing microbiological safety of fresh orange juice by fruit immersion in hot water and chemical sanitizers. | trials were conducted with hot water and chemicals to sanitize valencia oranges contaminated by natural microflora or inoculated with escherichia coli. microbial loads and sensory quality of fresh juice extracted from surface-heated fruit were also evaluated. e. coli on fruit surfaces was reduced by either hot water or chemical treatments. an estimated 5-log reduction of e. coli was attained by immersing inoculated fruit in hot water at 80 degrees c for 1 min or 70 degrees c for 2 min. immersing ... | 1999 | 10419268 |
| molecular characterization of an isolate of citrus tristeza virus that causes severe symptoms in sweet orange. | the complete sequence (19,249 nucleotides) of the genome of citrus tristeza virus (ctv) isolate sy568 was determined. the genome organization is identical to that of the previously determined ctv-t36 and ctv-vt isolates. sequence comparisons revealed that ctv-sy568, a severe stem-pitting isolate from california, has more than 87% overall sequence identity with ctv-vt, a seedling yellows isolate from israel. although sy568 has an overall sequence identity of 81% with ctv-t36, a quick decline isol ... | 1999 | 10541017 |
| ultrafiltration performance of heat-treated shamouti orange [citrus sinensis (l.) osbeck] juice. | during ultrafiltration of orange juice with inorganic membranes, heating of the juice prior to the filtration experiment resulted in a significant increase of the fouling indices. the effect of the irreversible fouling (rif) was always high, whereas the reversible fouling (rrf) depended on the treatment. it was clearly seen that fouling was reduced after pectin degradation, but the heat treatment applied to the juice before filtration still resulted in reduced fluxes. it is suggested that pectin ... | 1999 | 10552535 |
| involvement of proteins in cloud instability of shamouti orange [citrus sinensis (l.) osbeck] juice. | the present study provides evidence for the involvement of protein in cloud instability of natural orange juice. no heat-coagulable proteins were found in the serum. insoluble cloud matter (icm) was heat-flocculated following enzymatic pectin degradation (epd). the degree of flocculation depended on temperature (from approximately 50 to 75 degrees c) and was highest at ph 3.5. the fresh juice contained about 6.5 and 1.8 mg ml(-1) of icm and alcohol-insoluble solids of the serum (aiss), respectiv ... | 1999 | 10552536 |
| involvement of proteins in cloud instability of valencia orange [citrus sinensis (l.) osbeck] juice. | the present study examined the involvement of proteins in cloud flocculation of valencia orange juice. marked differences in cloud instability were found between juices of different harvest dates. heating of enzymatic pectin degraded juice from april and june harvests resulted in development of clumps and their precipitation. although the juice from both harvesting dates remained hazy, the juice of april harvest was more turbid than that from june. usually clarification increases as the temperat ... | 1999 | 10552537 |
| effect of juice extractor settings on juice cloud stability. | juice was extracted from valencia oranges using three different extractor settings. differential juice cloud stability was observed. soft-extracted juice was the most stable, and hard-extracted juice was the least stable. the medium-extracted juice had intermediate cloud stability. yearly (1997 versus 1998) differences were observed, but the relationship among the juices did not change. addition of protein extracts, obtained from each juice, to pasteurized juice also resulted in differential clo ... | 1999 | 10552577 |
| differentiation of several geographical origins in single-strength valencia orange juices using quantitative comparison of carotenoid profiles. | pure valencia orange (citrus sinensis) juices (64 samples) from spain, israel, belize, cuba, and florida, harvested during two seasons (1996-1997 and 1997-1998), were analyzed for their carotenoid profiles. the detection of saponified carotenoid pigments has been achieved and quantitated using a photodiode array detection monitored at 350, 430, and 486 nm. carotenoid pigments commonly found in the orange variety valencia have been separated on a polymeric c-30 column using a ternary gradient as ... | 1999 | 10552762 |
| fast hplc for the analysis of oxygen heterocyclic compounds of citrus essential oils. | a fast hplc method for the determination of the oxygen heterocyclic compounds of citrus essential oils was developed. five different oils were analyzed under identical conditions, by reversed-phase hplc with photodiode array detector, for a direct comparison of the composition of their oxygen heterocyclic fraction. analysis time was 7 min. the oils analyzed were lemon, bergamot, mandarin, sweet orange, and bitter orange. the method developed is good for rapid screening or fingerprinting of these ... | 1999 | 10552795 |
| antioxidant effectiveness as influenced by phenolic content of fresh orange juices. | several fresh orange juices, obtained from five different citrus sinensis (l.) osbeck varieties (three pigmented varieties, moro, sanguinello, and tarocco, and two blond varieties, valencia late and washington navel), were subjected to antioxidant profile determination (including total polyphenols, flavanones, anthocyanins, hydroxycinnamic acids, and ascorbic acid). the antioxidant activity of these juices was then assessed by means of different "in vitro" tests (bleaching of the stable 1,1-diph ... | 1999 | 10552879 |
| inactivation of heat-resistant pectinmethylesterase from orange by manothermosonication. | pectinmethylesterase of navel oranges shows two fractions greatly differing in thermostability. the most thermostable fraction accounts for approximately 10% of total activity. the thermal inactivation of this fraction follows first-order kinetics both in 5 mm, ph 3.5, citrate buffer and in orange juice at the same ph, showing a z value of 5.1 degrees c and an activation energy (e(a)) of 435 kj mol(-)(1) k(-)(1). the heat resistance of the enzyme is approximately 25-fold higher in the juice than ... | 1999 | 10563912 |
| microsporogenesis in a citrus interspecific tetraploid somatic hybrid and its fusion parents | microsporogenesis was analysed in a tetraploid somatic hybrid (sh) (2n=4x=36) of citrus and its diploid fusion parents (2n=2x=18), valencia sweet orange (c. sinensis l. osbeck) and femminello lemon (c. limon l. burm. f.). intergenomic pairing between lemon and orange occurred in the somatic hybrid which showed multivalent chromosome associations in diakinesis, although one quadrivalent was definitely because of a reciprocal translocation present in valencia. the behaviour of univalents was varia ... | 1999 | 10583538 |
| [genetic analysis of leaf-parent-type plants regenerated from somatic hybridization in citrus]. | the leaf indexes and stoma characteristics of leaf-parent-type regenerants were the same as those of the leaf parent rough lemon, but quite different from the somatic hybrids. leaf-parent-type plants were diploid (2n = 2x = 18). patterns of pox, ppo, got isozymes were identical to those of rough lemon. the plants were analyzed by using 54 arbitrary primers with polymorphisms. most of the primers (52) showed that leaf-parent-type plants were in concordance with rough lemon. however, with primer o ... | 1999 | 10589164 |
| the larvicidal activities of the peel oils of three citrus fruits against culex pipiens. | the larviciddal action of three ethanol extracts of peel oils of lemon, grapefruit and navel orange were tested against the early 4th instar larvae of culex pipiens and the resulting pupae. the lc50 were 18.5, 20.3 and 26.5 and the slope functions were 2.9, 2.9 and 3.9 respectively. the action of the lemon extract extended to the pupae which resulted from larvae exposed to sublethal dose. some of the pupae were unable to escape from the larval exuviae. | 1999 | 10605488 |
| high-temperature forced-air treatment alters the quantity of flavor-related, volatile constituents present in navel and valencia oranges. | a number of volatile compounds that contribute to orange flavor were quantified following high-temperature forced-air (htfa) treatment of the fruit to determine if a relationship exists between the flavor loss that is observed following htfa treatment and the volatile composition of the juice. following different durations of htfa treatment, fruit were stored for a period of 4 weeks and juiced and the juice subjected to headspace analysis using either a tenax/carbotrap column or a solid-phase mi ... | 1999 | 10606592 |
| identification of two chilling-regulated 1-aminocyclopropane-1-carboxylate synthase genes from citrus (citrus sinensis osbeck) fruit. | diurnal change in the temperature below or above 12.5 degrees c hastens the degreening of citrus peel and elicits the phytohormone ethylene production in citrus fruit. ethylene triggers the degradation of chlorophyll and synthesis of carotenoids in citrus peel. to investigate if ethylene is required for the degreening of citrus peel elicited by low temperatures, we studied the chilling-regulated gene expression of acc synthase, one of the key enzymes catalyzing ethylene biosynthesis. we isolated ... | 1999 | 10645719 |
| chlorophyll breakdown by chlorophyllase: isolation and functional expression of the chlase1 gene from ethylene-treated citrus fruit and its regulation during development. | we report on the isolation, functional expression and characterization of a cdna encoding chlorophyllase, the enzyme catalyzing the first step in the chlorophyll breakdown pathway. the chlase1 cdna from valencia orange (citrus sinensis cv. valencia) was obtained by rt-pcr using degenerate primers based on the amino acid sequence of the previously purified protein. chlase1 encodes a protein of 329 amino acids, including a sequence domain characterizing serine-lipases and a putative chloroplast-di ... | 1999 | 10652137 |
| distribution of tgg repeat-related sequences in 'trovita' orange (citrus sinensis osbeck) chromosomes | the clone pas"c" is a sequence related to m13 phage minisatellite sequences isolated from bovine dna. it contains tgg repeats, and related sequences are abundant in the citrus genome. fluorescent in situ hybridization (fish) signals detected using this clone as a probe showed that the tgg repeat-related sequences are found on all the citrus chromosomes, but are not found in citrus-specific cma+/dapi- heterochromatic regions located at the extreme ends of each chromosome. these data suggest that ... | 1999 | 10659794 |
| distribution of aflatoxin in almonds. 2. distribution in almonds with heavy insect damage. | the aflatoxin distribution function in individual insect-damaged neplus ultra almonds was determined and found to be the sum of two distributions. substantially all almonds exhibited a positive aflatoxin level between 0.02 ng/g (the detection level) and 0.3 ng/g, the precise form of this distribution depending on the lot studied. in addition, 1/1000 of the nuts showed contamination between 60 and 600 000 ng/g, independent of the lot. the latter distribution showed a smooth decrease with log conc ... | 2000 | 10691662 |
| molecular cloning and characterization of a novel gene encoding limonoid udp-glucosyltransferase in citrus. | we isolated a cdna clone encoding limonoid udp-glucosyltransferase (limonoid gtase) from the albedo of satsuma mandarin (citrus unshiu marc.) and investigated the contribution to limonoid glucoside accumulation in fruit. the isolated cdna clone (citlgt) was 1732 bp in length encoding 511 deduced amino acids with a predicted molecular mass of 57.5 kda. the products of in vitro translation from an expression vector had the limonoid gtase activity. southern blot analysis of genomic dna indicated th ... | 2000 | 10713266 |
| organochlorine pesticide residues in italian citrus essential oils, 1991-1996. | organochlorine pesticide contamination in 148 lemon essential oils, 123 sweet orange oils, 121 mandarin oils, and 147 bergamot oils produced in italy in the years 1991-1996 was studied by hrgc-ecd. confirmation analyses were carried out by gc-ms. tetradifon, dicofol and its decomposition product 4,4'-dichlorobenzophenone were found. over the course of the study dicofol and tetradifon residues steadily decreased; the percentage of contaminated samples reflects this course and decreases considerab ... | 2000 | 10725152 |
| some aspects of the biology and control using botanicals of the rice moth, corcyra cephalonica (stainton), on some pulses. | the life cycle of corcyra cephalonica was studied under ambient laboratory conditions (temperature range 27.5-30 degrees c and 60-73% r.h.) on groundnut, bambara groundnut and cowpea. the mean developmental period ranged from 33.2+/-0.2 to 45.3+/-1.8 days on whole, broken and powdered forms of the food media. egg hatchability was found to be 83%, while adult longevity ranged from 1.5+/-0.5 to 11.9+/-1.3 days for males and 1.5+/-0.5 to 16.5+/-1.2 days for females. sex ratio (male symbol:female sy ... | 2000 | 10758262 |
| chemical composition and selected functional properties of sweet orange (citrus sinensis) seed flour. | flour samples were prepared from dehulled and undehulled sweet orange (citrus sinensis) seeds. the flour samples were evaluated for proximate composition, mineral content and selected functional properties. proximate analysis showed a composition of 54.2% fat, 28.5% carbohydrate, 5.5% crude fiber, 3.1% crude protein and 2.5% ash for the dehulled orange seed flour (dry weight). mineral analyses showed high levels of calcium and potassium in flour samples. partially defatted and undefatted flour s ... | 1999 | 10798346 |
| role of furanocoumarin derivatives on grapefruit juice-mediated inhibition of human cyp3a activity. | with juices of grapefruit and related fruits, possible relationships between contents of six different furanocoumarins and extents of inhibition of microsomal cyp3a activity have been studied in vitro. microsomal cyp3a-mediated testosterone 6beta-hydroxylation was inhibited by the addition of a fruit juice (2.5%, v/v) from eight different grapefruit sources, two sweeties, three pomelos, and one sour orange, whereas no clear inhibition was observed with two sweet orange juices. the inhibitory com ... | 2000 | 10859150 |
| micropropagation of sweet orange, citrus sinensis osbeck. for the development of nucellar seedlings. | protocol for micropropagation of elite plants of sweet orange (citrus sinensis) through nucellar embryo culture has been standardized. three to four nucellar embryos and a zygotic embryo could be excised from a single mature seed and successfully generated as healthy plants in basal ms medium. ms medium supplemented with naa (1 mg/l) or 2, 4.d (1 mg/l) promoted callus development in both nucellar and zygotic embryos. ga3 (1 mg/l) enriched medium induced plantlets initiation but their growth was ... | 2000 | 10927871 |
| [two interspecific somatic hybrid plants regenerated via protoplast electro-fusion]. | protoplasts isolated from cell suspension cultures of 'bonnaza' navel orange (citrus sinensis l. osbeck) were electrically fused with mesophyll protoplasts of rough lemon (citrus jambhiri lush) and goutou orange (citrus aurantium l.) respectively. plants regenerated from both fusion combinations. chromosome counting of randomly selected fifty two globular embryoids as well as all the regenerated seventy four plants from bonnaza navel + rough lemon revealed that twenty six embryoids were tetraplo ... | 2000 | 10976322 |
| specificities of functionally expressed chalcone and acridone synthases from ruta graveolens. | the common rue, ruta graveolens l., expresses two types of closely related polyketide synthases that condense three malonyl-coas with n-methylanthraniloyl-coa or 4-coumaroyl-coa to produce acridone alkaloids and flavonoid pigments, respectively. two acridone synthase cdnas (acs1 and acs2) have been cloned from ruta cell cultures, and we report now the cloning of three chalcone synthase cdnas (chs1 to chs3) from immature ruta flowers. the coding regions of these three cdnas differ only marginally ... | 2000 | 11054106 |
| flavonoids in pigmented orange juice and second-pressure extracts. | flavanone glycosides (fgs) and polymethoxylated flavones (pmfs) have been studied in pigmented orange (citrus sinensis) juices and second-pressure extracts (spes) by high-performance liquid chromatography and diode array detector. detection was performed simultaneously at two different wavelengths: 278 nm (for determination of fgs) and 325 nm (for determination of pmfs). qualitative distribution patterns of fgs and pmfs in juices and spes were similar, although the quantitative results are quite ... | 2000 | 11087509 |
| lc-ms for the identification of oxygen heterocyclic compounds in citrus essential oils. | the oxygen heterocyclic compounds (coumarins, psoralens and polymethoxylated flavones) present in the nonvolatile residue of the essential oils of mandarin, sweet orange, bitter orange, bergamot and grapefruit were analysed with an hplc/api/ms system equipped with an apci probe in positive mode. the use of hyphenated techniques, such as lc/ms provides a great information about the content and nature of constituents of natural complex matrices, such as essential oils. in this work, ms spectra wer ... | 2000 | 11108548 |
| ambient odor of orange in a dental office reduces anxiety and improves mood in female patients. | essential oils have been used as remedies for a long time in different cultures across the world. however, scientific proof of such application is scarce. we included 72 patients between the ages of 22 and 57 while waiting for dental treatment in our study. the participants were assigned to either a control group (14 men, 23 women) or to an odor group (18 men and 17 women). ambient odor of orange was diffused in the waiting room through an electrical dispenser in the odor group whereas in the co ... | 2016 | 11134689 |
| incidence and epidemiology of citrus tristeza virus in the valencian community of spain. | the first outbreak of citrus tristeza disease in spain caused by citrus tristeza virus (ctv) was recorded in 1957 in the valencian community (vc). in total c. 40 million trees, mainly of sweet orange and mandarin grafted on sour orange rootstocks, declined due to ctv. large-scale surveys in different municipalities of the vc indicated that the disease spread very fast. incidence increased from 11% in 1989 to 53% in 1998. toxoptera aurantii and aphis spiraecola (inefficient aphid vectors of ctv) ... | 2000 | 11137164 |
| characterization of the low affinity transport system for no(3)(-) uptake by citrus roots. | three-month old citrange troyer (hybrid of citrus sinensis x poncirus trifoliata) seedlings were grown hydroponically and, after a period of no(3)(-) starvation, plants were transferred to solutions enriched with k(15)no(3) (96% atoms 15n excess) to measure 15no(3)(-) uptake rates as a function of external 15no(3)(-) concentrations. two different no(3)(-) uptake systems were found. between 1 and 50 mm 15no(3)(-) in the uptake solution medium, the uptake rate increased linearly due to the low aff ... | 2000 | 11164581 |
| biochemical basis of color as an aesthetic quality in citrus sinensis. | the biochemical basis of color as an aesthetic quality in mature fruit of navel and valencia orange (citrus sinensis) was determined. saponification of the two major color-imparting components resolved by thin-layer chromatography, followed by reversed-phase high-performance liquid chromatography, revealed that these comprised acyl esters of (9z)-violaxanthin and beta-citraurin. identification of the chromophores was based on cochromatography and online spectral analysis. the color quality of fl ... | 2001 | 11170592 |
| insecticidal properties of volatile extracts of orange peels. | statistical studies using the randomised complete block design with four replicates showed that volatile extracts of two species of orange peel--citrus sinensis (sweet orange) and citrus aurantifolia (lime) had insecticidal activity against mosquito, cockroach and housefly. insecticidal activity was better after 60 min than at 30 min spraying of rooms. volatile extracts of c. sinensis showed greater insecticidal potency, while the cockroach was the most susceptible to the orange peels among the ... | 2001 | 11198181 |
| in vitro anti-mycotic activity of some medicinal plants containing flavonoids. | aqueous, ethanolic and petroleum ether extracts of citrus sinensis l. (osbeck), euphrasia officinalis l., glycyrrhiza glabra l., matricaria recutita l., rosa canina l. and ruta graveolens l. have been studied. the antimycotic activity was evaluated "in vitro" on strains of candida albicans isolated from clinical samples obtained in the course of acute vaginitis. | 2000 | 11213443 |