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cloning of the cole3-ca38 colicin and immunity genes and identification of a plasmid region which enhances colicin production.the colicin and immunity genes of plasmid cole3-ca38 have been localized by characterization of bacteria carrying its cloned restriction fragments. they are within a 3.14-kb ecori segment, such that the immunity gene contains the kpni site, and the colicin gene is adjacent to it within a 2.1-kb kpni-hincii segment. the immunity gene and one end of the colicin gene are in the region of cole3-ca38 which is not homologous to the closely related plasmid cole2-p9. a 0.64-kb pvui-ecori segment of the ...19826293928
comparative nucleotide sequences encoding the immunity proteins and the carboxyl-terminal peptides of colicins e2 and e3.using the m13 dideoxy sequencing technique, we have established the dna sequences of colicins e2 and e3 which encompass the receptor-binding and the catalytic domains of each of the nucleases, and their immunity (imm) genes. the imm gene of plasmid cole2-p9 is 255 bp long and is separated from the end of the col gene by a dinucleotide. this gene pair is arranged similarly in plasmid cole3-ca38 except that the intergenic space is 9 bp and the e3 imm gene is one codon shorter than its e2 counterpa ...19846095211
characterization and nucleotide sequence of a colicin-release gene in the hic region of plasmid cole3-ca38.downstream from its colicin and immunity genes (col. imm), escherichia coli plasmid cole3-ca38 contains a 0.81-kb dna segment, the hic region, which is required for high colicin production. characterization of derived plasmids, carrying the col-imm operon but varying in the hic region, showed that the latter functions in lacuna production, colicin release, cell death, and lysis. the hic gene expression after induction was shown to be dependent on the col gene promoter. the nucleotide sequence of ...19846092219
colicin e3 and its immunity genes.a dna segment of plasmid cole3-ca38 was cloned into pbr328 and its nucleotide sequence was determined. this segment contains the putative promoter-operator region, the structural genes of protein a (gene a) and protein b (gene b) of colicin e3, and a part of gene h. just behind the promoter region, there is an inverted repeat structure of two 'sos boxes', the specific binding site of the lexa protein. this suggests that the expression of colicin e3 is regulated directly by the lexa protein. gene ...19853889348
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