Publications

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[sensitivity of mycoplasmas to spiramycin]. 19724669219
[stimulating effect of langat virus on reproduction of mycoplasma laidlawii in combined infection of a primary culture of chick embryo cells]. 19724672317
fungistatic activity of lucerne saponins and digitonin as related to sterols. 19724676387
relations between lipid composition and permeability of membranes. 19724680245
thiopeptin, a new feed-additive antibiotic: biological studies and field trials.thiopeptin is a new antibiotic, produced by streptomyces tateyamensis and developed solely for animal use as a feed additive. the antibiotic content in animal tissue and feed was assayed in terms of the antimicrobial activity against mycoplasma laidlawii a. this antibiotic was found to be relatively nontoxic in rats and mice. in chickens, this antibiotic is excreted into feces within 48 hr of administration and is not absorbed in tissue. it is well tolerated in both broilers and swine and is hig ...19724680812
freeze-fractured acholeplasma laidlawii membranes: nature of particles observed.freeze-fracture of acholeplasma laidlawii membranes from cells incubated in the presence of puromycin or omission of amino acids reveals a decrease in the number of particles between 50 and 100 angstroms in the hydrophobic fracture plane, which strongly suggests that these particles are protein. additional evidence indicates that they may be involved in substrate transport.19734682132
[antigenic relationship of saprophytic and plant mycoplasmas]. 19734683168
weak association of glucosamine-containing polymer with the acholeplasma laidlawii membrane. 19734684605
chemical composition and serology of mycoplasma pneumoniae lipids. 19734689627
circular dichroism analysis of native and reaggregated mycoplasma membranes. 19734706839
molecular model for phase transition in biological membranes. 19734705755
[carbohydrate transport in mycoplasma laidlawii cells]. 19734696486
freeze-etch electron microscopy of erythrocytes, acholeplasma laidlawii cells and liposomal membranes after the action of filipin and amphotericin b. 19734690870
on the phosphorylation of sugars in acholeplasma laidlawii. 19734709990
polyacrylamide gel electrophoresis of mycoplasma proteins in sodium dodecyl sulphate. 19734723070
further studies on the morphology and composition of mycoplasmatales virus-laidlawii 2. 19734735309
isolation of mycoplasmatales virus-laidlawii 3, a new virus infecting acholeplasma laidlawii. 19734736684
the effect of the polar headgroup on the lipid-cholesterol interaction: a monolayer and differential scanning calorimetry study. 19734711186
properties of a fructose-1,6-diphosphate-activated lactate dehydrogenase from acholeplasma laidlawii type a.acholeplasma laidlawii a possesses a nicotinamide adenine dinucleotide (nad)-dependent l(+)-lactate dehydrogenase (ldh) which is activated specifically by low concentrations of fructose-1, 6-diphosphate (fdp). studies with partially purified enzyme show that the kinetic response to fdp is hyperbolic. the enzyme is inhibited by inorganic phosphate, adenosine triphosphate, and high concentrations of reduced nad (nadh). low activity is demonstrable in the absence of fdp at ph 6.0 to 7.2, but fdp is ...19734712565
spiroplasmas and acholeplasmas: multiplication in insects.the helical wall-free microorganism, spiroplasma citri, which is associated with citrus stubborn, a disease with no known vector, multiplied in the leafhopper vector of corn stunt but multiplied to higher titer in the vector of aster yellows and decreased the longevity of that insect. acholeplasma laidlawii and a. granularum also multiplied in both leafhoppers.19734796196
isolation and purification of tdna from mycoplasma. 19734803254
the effect of alterations in fatty acid composition and cholesterol content on the nonelectrolyte permeability of acholeplasma laidlawii b cells and derived liposomes. 19734719142
studies of sugar transport in acholeplasma laidlawii. 19734772677
selective simultaneous proton magnetic resonance labelling of hydrophilic and hydrophobic regions of phospholipid vesicles. effect of polar and apolar reagents. 19734795847
intracellular replication of mycoplasmavirus mvl51.the intracellular replication of mvl51, a group l1 mycoplasmavirus, was investigated. the single-stranded parental dna was found to enter the cell and become converted to double-stranded dna. this replicated to yield additional double-stranded dna molecules. the parental viral dna was found to leave the replication complex and become associated with large molecular weight dna not involved with viral replication. progeny viral dna formed from the double-stranded dna and an intracellular accumulat ...19744821487
radiobiological properties of acholeplasma laidlawii b. 19744822050
the effect of alterations in the physical state of the membrane lipids on the ability of acholeplasma laidlawii b to grow at various temperatures. 19744830862
incorporation of 3h-uridine and 3h-uracil into rna: a simple technique for the detection of mycoplasma contamination of cultured cells. 19744817725
replacement of the octadecenoic acid growth-requirement for acholeplasma laidlawii a by cis-9,10-methylenehexadecanoic acid, a cyclopropane fatty acid. 19744820352
interaction of mycoplasma with viruses. i. primary adsorption of virus is ionic in mechanism.we have studied the adsorption of the gourlay acholeplasma virus mvl-1 to the host cell acholeplasma laidlawii ja-1. successful adsorption depends primarily on some unknown action of the serum factor in the medium, but evaluation of various physical parameters indicates clearly that given this factor the kinetics is pseudo first order (k = 3 x 10(-9) cm(3)/min) and the mechanism ionic. chiefly important are the ionic strength of the cation and ph (optimal na(+) = 0.08 m, ph = 6). the system seem ...19744824711
a simplified method for the detection of mycoplasma. 19744858125
deoxyribonucleate binding and transformation in mycoplasma laidlawii. 19684865478
physiological and serological comparisons among strains of mycoplasma granularum and mycoplasma laidlawii.mycoplasma granularum strains grew on a medium devoid of animal serum or of serum fractions containing sterols; all strains possessed properties, including carotenoid biosynthesis, similar to those described for m. laidlawii. some common antigenic components were noted among m. granularum and m. laidlawii strains by indirect fluorescent-antibody tests. the growth of m. granularum strains was slightly inhibited by antiserum to m. laidlawii pg-8, and the electrophoretic patterns of cell proteins o ...19684870272
macromolecular synthesis and thymineless death in mycoplasma laidlawii b.the relationships between macromolecular synthesis and viability have been studied in the pleuropneumonia-like organism mycoplasma laidlawii b adapted to a semidefined grwoth medium. this organism exhibited an absolute growth requirement for the nucleosides uridine and thymidine, a partial requirement for guanosine and deoxyguanosine, but no requirement for adenosine, deoxyadenosine, cytosine, and deoxycytosine. cytosine and deoxycytosine partially satisfied the requirement for uridine. loss in ...19684881702
an ultrastructural study of mycoplasma laidlawii type a and two strains of mycoplasma laidlawii isolated from the oviducts of dairy cows. 19684888099
dna replication in mycoplasma laidlawii b. 19694890600
cholesterol in mycoplasma membranes. i. kinetics and equilibrium studies of cholesterol uptake by the cell membrane of acholeplasma laidlawii. 19744841672
rapid determination by light scattering of growth parameters of mycoplasma laidlawii in liquid media.an impediment to progress in the study of the course of growth, the effects of medium components, antibiotics, etc., of mycoplasma has been the cumbersome methods of growth measurement currently in use. heretofore, it required the plating of numerous samples during growth, at least in triplicate, after appropriate dilution, followed by a delay of 2 to 3 days before the colonies developed so that counts could be made. we applied the technique of light scattering to measure the growth of mycoplasm ...19694896103
cholesterol requirement of mycoplasmas.cholesterol requirement for growth of mycoplasmas was tested in a serum-free medium supplemented with albumin, l-arginine, palmitic acid, and various concentrations of cholesterol dissolved in tween 80. in cases in which tween 80 was shown to inhibit growth, the test medium was supplemented with cholesterol dissolved in ethanol. of the 31 species examined, all but mycoplasma laidlawii, m. granularum, and mycoplasma species strain s-743 exhibited a growth response to cholesterol. no requirement f ...19704911537
acholeplasma axanthum, sp. n.: a new sterol-nonrequiring member of the mycoplasmatales.mycoplasmas recovered from tissue cultures and previously shown to belong to the sterol-nonrequiring group of mycoplasmas have been further characterized. the biological and serological properties of these strains show them to be clearly distinct from acholeplasma laidlawii and a. granularum, two species of sterol-nonrequiring mycoplasmas recently reclassified. it is proposed that the newly described mycoplasmas be designated acholeplasma axanthum, sp. n.19704919991
immunogenicity of mycoplasma pneumoniae glycolipids: a novel approach to the production of antisera to membrane lipids.the glycolipid haptens of mycoplasma pneumoniae were bound to membrane proteins of acholeplasma laidlawii (formerly mycoplasma laidlawii) by reaggregation. this process consisted of the solubilization of lipid-depleted a. laidlawii membranes and m. pneumoniae glycolipids in 20 mm sodium dodecyl sulfate, and dialysis of the mixed solutions against 20 mm mg(2+). the hybrid reaggregate, collected by centrifugation, was highly immunogenic in rabbits, eliciting the production of a high titer of antib ...19704943173
isolation and identification of porcine mycoplasma in switzerland. 19714946169
[the effects of glucose on mycoplasma]. 19714947989
mycoplasmas in cell cultures from rheumatoid synovial membranes.ten strains of myocoplasmas were recovered from cultures of synovium or cultures inoculated with synovial fragments from rheumatoid arthritis and one from osteo-arthritis. the source of the organisms is not known. patients with rheumatoid arthritis had no complement-fixing antibody and no fluorescent staining antibody against the mycoplasmas isolated and no mycoplasma antigen was detected by immunofluorescence in sections of synovia and in synovial fluids.the strains isolated were of two main se ...19714948122
immunological analysis of mycoplasma membranes.the antigens responsible for the production of antibodies to mycoplasma laidlawii and m. gallisepticum causing growth and metabolic inhibition of these organisms were localized in the cell membrane. various membrane fractions were tested for serological activity. membrane lipids were completely or almost completely inactive, whereas several preparations of defatted membrane proteins retained some serological activity, shown by their ability to stimulate metabolic inhibition antibody in rabbits a ...19694981055
a membrane-bound lysophospholipase from mycoplasma laidlawii strain b. 19714993729
[ultrastructure and development of colonies of a strain of mycoplasma laidlawii in solid media]. 19715002964
incorporation of penicillinase into reaggregated mycoplasma membranes. 19715003616
effect of fatty acyl chain length on some structural and functional parameters of acholeplasma membranes. 19744844283
isolation of acholeplasma laidlawii from guinea-pigs. 19744852593
characteristics of a new sterol-nonrequiring mycoplasma.two mycoplasma strains recovered from tissue culture environments were found to grow in complex media devoid of serum or serum fractions containing cholesterol and in a cholesterol-free synthetic medium. neither strain was capable of synthesizing pigmented carotenoids, although these compounds are present in, and characteristic of, other sterol-nonrequiring mycoplasmas. serological tests and an analysis of their cell protein patterns obtained by gel electrophoresis indicated that the isolates we ...19694892385
the effect of cholesterol and epicholesterol incorporation on the permeability and on the phase transition of intact acholeplasma laidlawii cell membranes and derived liposomes. 19725011001
distribution of mycoplasmas in the non-pneumonic bovine respiratory tract. 19725018036
the molecular structure of the membrane of acholeplasma laidlawii. 19725041943
13 c nmr studies of lipids in bilayers and membranes. 19725041946
[morphological properties of mycoplasma laidlawii]. 19725042100
lipid mobility in acholeplasma membranes using deuteron magnetic resonance. 19725041152
studies on the biological properties of polyene antibiotics. evidence for the direct interaction of filipin with cholesterol. 19725012767
polyacrylamide-gel electrophoresis of cell proteins of mycoplasma isolated from cattle and horses. 19725017869
characterization of the plasma membrane of mycoplasma laidlawii. 8. effect of temperature shift and antimetabolites on k + transport. 19725044055
dark and light repair in ultraviolet-irradiated acholeplasma laidlawii. 19725060072
are mycoplasma membrane proteins affected by variations in membrane fatty acid composition? 19725044067
characterization of the plasma membrane of mycoplasma laidlawii. ix. isolation and characterization of the membrane polyhexosamine. 19725049002
thermal-turbidimetric studies of membranes from acholeplasma laidlawii. 19725070088
some studies on the fatty acid composition of total lipids and phosphatidylglycerol from acholeplasma laidlawii b and their relation to the premeability of intact cells of this organism. 19725070656
[study of mycoplasma isolated from cases of swine pneumonia]. 19725081406
selective solubilization with tween 20 of membrane proteins from acholeplasma laidlawaii. 19725082994
phase transitions of phospholipid bilayers and membranes of acholeplasma laidlawii b visualized by freeze fracturing electron microscopy. 19725082995
altered incorporation of nucleic acid precursors by mycoplasma-infected mammalian cells in culture. 19725058910
improved activity sedimentation method. 19725062881
mycoplasma isolations from clinically normal cats. 19725063126
lipid bilayer structure in the membrane of mycoplasma laidlawii. 19715088924
peptidase activity in the membranes of mycoplasma laidlawii. 19715117556
the microscopic examination of the apollo-11 and -12 sample and the quest for life forms. 19715142391
structural comparisons of native and reaggregated membranes from mycoplasma laidlawii and erythrocytes by x-ray diffraction and nuclear magnetic resonance techniques. 19715159791
structural comparisons of native and reaggregated membranes from mycoplasma laidawii and erythrocytes using a fluorescence probe. 19715159792
immunogenicity of cytolipin h aggregated with acholeplasma laidlawii membrane proteins. 19715165763
[etiology and diagnosis of porcine mycoplasmosis. 3. pathological, anatomical and histological studies of experimentally infected suckling pigs]. 19715131929
characterization of the mycoplasma membrane proteins. i. reaggregation of solubilized membrane proteins of acholeplasma laidlawii. 19715141123
[biochemical investigations on 10 strains of mycoplasma laidlawii]. 19715105631
[effect of mycoplasma-viral infection of a primary culture of chick embryo cells on interferon production induced by langat virus]. 19715167284
induction of alpha-glucosidase in mycoplasma laidlawii a. 19715283618
kinetics of sodium dodecyl sulfate solubilization of mycoplasma laidlawii plasma membranes.the kinetics of sodium dodecyl sulfate solubilization of aqueous suspensions of mycoplasma laidlawii membranes have been investigated by light scattering in a stopped-flow apparatus. there was evidence of direct interaction between the membranes and sodium dodecyl sulfate micelles above the critical micelle concentration, although of lower order kinetically than with monomeric dodecyl sulfate anions below the critical micelle concentration. the activation energy remained the same in either case, ...19715289357
use of dialyzing culture technique for high yield of mycoplasma.pollock, mary e. (university of minnesota, minneapolis). use of dialyzing culture technique for high yield of mycoplasma. j. bacteriol. 90:1682-1685. 1965.-the saprophytic pleuropneumonia-like organism, mycoplasma laidlawii, type a, was cultivated within a dialyzing membrane suspended in a reservoir of uninoculated medium. generation time was not decreased by this method, but the period of active growth was prolonged, and the final yield was 10-fold that attained by the usual cultural methods. a ...19655322724
morphology of mycoplasma laidlawii type a. i. comparison of electron microscopic counts with colony-forming units.anderson, d. l. (university of minnesota, minneapolis), m. e. pollock, and l. f. brower. morphology of mycoplasma laidlawii type a. i. comparison of electron microscopic counts with colony-forming units. j. bacteriol. 90:1764-1767. 1965.-cells of mycoplasma laidlawii a grown in dialyzing flask cultures were counted with the electron microscope by use of a spray technique which deposited mixtures of polystyrene latex of known concentration and m. laidlawii in discrete droplet patterns on specimen ...19655322728
[serologic differentiation between acholeplasma laidlawii (mycoplasma laidlawii) and acholeplasma granularum (mycoplasma granularum) strains using agglutination and precipitation]. 19715169114
membrane subunits of mycoplasma laidlawii and their assembly to membranelike structures. 19655216355
calorimetric evidence for the liquid-crystalline state of lipids in a biomembrane.both membranes of mycoplasma laidlawii and water dispersions of protein-free membrane lipids exhibit thermal phase transitions that can be detected by differential scanning calorimetry. the transition temperatures are lowered by increased unsaturation in the fatty acid residues, but in each case they are the same for membranes and lipids. the transitions resemble those observed for synthetic lipids in the lamellar phase in water, which arise from melting of the hydrocarbon chains within the phos ...19695257955
the effect of long chain fatty acid isomers on growth, fatty acid composition and osmotic fragility of mycoplasma laidlawii a. 19695374018
proposal for classifying organisms related to mycoplasma laidlawii in a family sapromycetaceae, genus sapromyces, within the mycoplasmatales. 19695391433
repair replication of dna in ultraviolet irradiated mycoplasma laidlawii b. 19695358643
observations on membranes of mycoplasma laidlawii strain b.the cytoplasmic membrane of mycoplasma laidlawii strain b is solubilized by anionic and nonionic detergents, succinylation, phospholipase a, alkaline phosphatase, trypsin, and chymotrypsin. cationic detergents are without effect, as are chelating agents, even in the presence of high concentrations of monovalent cation. the detergent-solubilized membrane exhibits one peak in the analytical ultracentrifuge, but the sedimentation coefficient is dependent upon concentration of detergent. simple dial ...19695361209
incorporation and elongation of fatty acid isomers by mycoplasma laidlawii a. 19705412665
x-ray diffraction studies of phase transitions in the membrane of mycoplasma laidlawii. 19705413340
the relationship between fatty acid structure and the positional distribution of esterified fatty acids in phosphatidyl glycerol from mycoplasma laidlawii b. 19705417177
isolation of a virus infecting a strain of mycoplasma laidlawii. 19705418254
deoxyribonucleic acid homology and relative genome size in mycoplasma.the deoxyribonucleic acid homologies of mycoplasma laidlawii type a and type b, m. pulmonis (#47 and #63), and m. hominis were determined by membrane methodology. the homology data revealed a difference in genome size between m. laidlawii type a and type b. this difference also held with stringent conditions of annealing (high temperature). little or negligible homology was shown to exist between the m. laidlawii strains type a and type b and m. pulmonis strains 47 and 63 and m. hominis. m. homi ...19695370271
characterization of the plasma membrane of mycoplasma laidlawii. vii. phase transitions of membrane lipids. 19705473498
native and reformed mycoplasma laidlawii membranes compared by freeze-etching. 19705473500
the effect of alterations in fatty acid composition and cholesterol content on the permeability of mycoplasma laidlawii b cells and derived liposomes. 19705473512
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