PMID(sorted descending)
temperature-sensitive mutants of mycoplasma gallisepticum.mycoplasma gallisepticum (mg) s6 strain was treated with nitrosoguanidine to obtain temperature-sensitive mutants. of the 101 colonies screened, 4 were found to be temperature sensitive. these mutants and the wild type organisms were serologically and morphologically identical. three of the 4 mutants failed to produce air sac lesions when inoculated directly into air sacs of the chicken.19846699219
mycoplasma gallisepticum (mg)--laboratory and field studies evaluating the safety and efficacy of an inactivated mg bacterin.a highly antigenic isolate of mycoplasma gallisepticum (mg) was utilized in the production of an inactivated, oil-emulsified mg bacterin (mgb). laboratory tests indicated that the bacterin was capable of protecting chickens from clinical signs of mg caused by intrasinus challenge with the r, s-6, pg-31, or 1150 strain of mg. vaccinated turkeys also were protected from clinical signs of disease when challenged with mg. use of the mgb in chickens under laboratory conditions resulted in a reduction ...19836688951
synthesis of adenylate nucleotides by mollicutes (mycoplasmas).cultures of the mollicutes (mycoplasma) acholeplasma laidlawii b, acholeplasma morum, mycoplasma bovis, mycoplasma arginini, mycoplasma fermentans and mycoplasma gallisepticum, representing four metabolic groups, were sampled at intervals over a 40 to 50 h period and assayed for the numbers of c.f.u., changes in ph and glucose concentration, and concentrations of atp, adp, amp, lactate and pyruvate. the adenylate energy charge (eca), the mean generation time, and the number of nmol of atp (mg dr ...19836655457
mycoplasma gallisepticum infection in wild-type turkeys living in close contact with domestic fowl.mycoplasma gallisepticum was isolated from 2 wild-type turkeys (meleagris gallopavo) and 1 domestic turkey living in close contact on a farm in tehama county, california. sinusitis was detected in 2 of 14 wild-type turkeys and in 1 of 12 feral broad-breasted bronze turkeys, but in none of several chickens on the premises. the entire mixed flock was captured, sinus aspirates were collected from affected birds, and blood samples were obtained from all birds for serologic testing. blood samples als ...19836643238
indirect micro-enzyme-linked immunosorbent assay for the detection of antibodies to mycoplasma synoviae and m. gallisepticum.the sensitivity and specificity of the indirect micro-enzyme-linked immunosorbent assay (elisa) was compared with that of the rapid serum-plate test (rspt) and the hemagglutination-inhibition test (hit) in detecting antibodies to mycoplasma gallisepticum (mg) and m. synoviae (ms). membrane antigens of mg strain s6 and ms strain nel 61800 were used. elisa was performed with single ms and single mg antigens and a combined ms/mg antigen. the ms-elisa was as sensitive as the ms-rspt and more sensiti ...19836639552
forms of leg abnormality observed in male broilers fed on a diet containing 12.5 percent rapeseed meal.the incidence of leg abnormalities was studied in 216 male ross i broilers, fed for 10 weeks on a diet containing 12.5 per cent extracted rapeseed. regular serological examination showed that the birds remained free from mycoplasma gallisepticum, mycoplasma synoviae and avian reovirus throughout the period of investigation. post mortem examination and radiographs were performed when birds were culled due to leg deformities or at the end of the experiment. leg abnormalities were seen in 19.4 per ...19836635344
mycoplasma gallisepticum in culture with biosilon microcarrier beads.various dilutions of mycoplasma gallisepticum were cultured in the presence of biosilon microcarrier beads. the microcarriers did not affect the recoverability or the growth rate of m. gallisepticum. cultures attained a higher density in the presence of microcarriers. the initiation of a culture could be accomplished by the transfer of one bead from a microcarrier culture.19836630451
bacterins and vaccines for the control of mycoplasma gallisepticum.the preferred method for the control of mycoplasma gallisepticum is eradication. unfortunately, the advent of multiple-age farms for poultry production has made eradication impractical after the organism has been introduced. live vaccination with the f strain of m. gallisepticum during the rearing period has been used to prevent egg production losses. more recently, an inactivated oil emulsion bacterin has become available commercially. vaccine and bacterin offer protection against egg productio ...19846548986
vaccination of turkeys against air-sac infection with a temperature-sensitive mutant of mycoplasma gallisepticum.turkeys were vaccinated with temperature-sensitive (ts) mutants of mycoplasma gallisepticum (mg) to determine pathogenicity and immunogenicity. ts 37 was apathogenic yet immunogenic to turkeys, ts 100 was highly pathogenic, and ts 102 was slightly pathogenic and nonimmunogenic. five or 7 weeks after intranasal vaccination of turkeys with the ts 37 mutant, a highly statistically significant resistance against intra-air-sac challenge with the s6 strain of mg was observed.19846525130
uptake and transbilayer distribution of phosphatidylcholines in mycoplasma gallisepticum and their effect on cell morphology.mycoplasma gallisepticum cells grown in a serum-free medium incorporated large amounts of egg-phosphatidylcholine (egg-pc), dioleoylphosphatidylcholine (dopc) or dipalmitoylphosphatidylcholine (dppc) added to the growth medium. egg-pc and dopc were incorporated at a high rate and to a large extent and were modified by the organisms, whereas dppc was incorporated at a lower rate and to a lesser extent and was not modified by the cells. the lactoperoxidase-mediated radioiodination applied to study ...19846511357
utilization of temperature-sensitive mutants of mycoplasma gallisepticum to prevent air sac infections.three stable temperature-sensitive (ts) mutants were obtained by treating the s6 strain of mycoplasma gallisepticum with 50 micrograms/ml of nitrosoguanidine. these mutants were morphologically and serologically indistinguishable from the parent s6 strain. mutants ts 37 and ts 102 were apathogenic, and ts 100 was moderately pathogenic to chickens when inoculated directly into the air sac. to turkeys, ts 37 remained apathogenic, ts 102 was slightly pathogenic, and ts 100 was highly pathogenic, in ...19846511327
early stages in the interaction between mycoplasma gallisepticum and the chick trachea, as related to pathogenicity and immunogenicity.mycoplasma strains, which occur naturally or as the result of laboratory manipulation, differ markedly in biological properties such as pathogenicity, infectivity, transmissibility, and immunogenicity. these properties are clinical expressions of a series of complex interactions between the mycoplasma organisms and the host. we have focused on the early interactions occurring at the level of the tracheal mucosa, with the intent of identifying mycoplasma gallisepticum strains possessing a combina ...19846511325
resistance of chickens immunized against mycoplasma gallisepticum is mediated by bursal dependent lymphoid cells.newly hatched chickens were significantly protected against challenge by the virulent s6 strain of mycoplasma gallisepticum after vaccination with the ts 100 mutant. removal of the thymus did not abolish the protective effect of the vaccine, but removal of the bursa of fabricius did. the results suggest that the resistance induced by vaccine is mediated by the bursal-dependent lymphoid cells.19846495612
an epornitic of mycoplasma gallisepticum in turkeys.a major epornitic of mycoplasma gallisepticum occurred in the monroe, north carolina, area between january and june of 1983. the outbreak involved 304,000 turkeys of various ages, which were slaughtered in the eradication program at a cost of more than $550,000 to growers and poultry companies. an infected peafowl was the likely source of infection on the first farm. traffic between farms by growers and company personnel was theorized to be the means of further spread.19846487195
infectious sinusitis in turkeys at ibadan, nigeria.thirty out of 70 imported broad-breasted white turkeys with severe sinusitis were both culturally and serologically positive for mycoplasma gallisepticum. infectious sinusitis due to m. gallisepticum was therefore diagnosed. a low mortality of 5.7% of the total flock was recorded. this appears to be the first published report on m. gallisepticum isolation in turkeys in nigeria.19846487193
a comparison of the methods of the european pharmacopoeia for the detection of mycoplasma gallisepticum and mycoplasma synoviae.the methods specified in the european pharmacopoeia for the detection of mycoplasma synoviae and mycoplasma gallisepticum as contaminants of vaccines were compared by investigating the serological responses of chicks inoculated with dilutions of mycoplasma cultures, these cultures being titrated in parallel in vitro. inoculation by the intrathoracic route proved to be as sensitive as, or more sensitive than the other methods and was of similar sensitivity to the in vitro titrations for both agen ...19846480614
two cholesterol pools in acholeplasma laidlawii membranes.cholesterol exchange kinetics between [14c]cholesterol-labeled acholeplasma laidlawii and mycoplasma gallisepticum cells and phosphatidylcholine-cholesterol vesicles followed a biphasic curve, with faster exchange rates for a. laidlawii. the same biphasic curve was obtained with isolated membranes. cholesterol exchange between lipid vesicles and a. laidlawii cells depleted of phospholipids by phospholipase a2, fitted a monophasic linear curve. the data support the hypothesis that the biphasic ch ...19846479337
effect of trichothecenes on growth and intracellular pool size of mycoplasma gallisepticum.the mycotoxin t-2 inhibited the growth of mycoplasma gallisepticum. the growth inhibition was most pronounced with the hydrophobic derivatives t-2 acetate and very little with the hydrophilic t-2 tetraol. the toxin had no effect on the biosynthesis of either protein, dna, rna or complex lipids but markedly reduced the intracellular pool size of soluble low molecular mass precursors. it seems that t-2 acetate, by virtue of its hydrophobic nature, may accumulate within the lipid backbone affecting ...19846479334
decreases in rates of lipid exchange between mycoplasma gallisepticum cells and unilamellar vesicles by incorporation of sphingomyelin.the kinetics of exchange of radiolabeled cholesterol and phospholipids between mycoplasma gallisepticum cells and an excess of small unilamellar phospholipid/cholesterol vesicles were studied using cells enriched with different phospholipid classes but the same fatty acyl content. the rates of the rapidly exchanging cholesterol and phospholipid pools were markedly slower in sphingomyelin-containing cells than in phosphatidylcholine-containing cells. the decreased rates of spontaneous cholesterol ...19846469983
demonstration of mycoplasma gallisepticum in tracheas of healthy carrier chickens by fluorescent-antibody procedure and the significance of certain serologic tests in estimating antibody response.chickens naturally infected with mycoplasma gallisepticum (mg) were cultured by tracheal swab. although the chickens showed no signs of disease, they remained mg carriers many months after the acute phase of infection. when mg was isolated from tracheas, the agent was demonstrated also in smear preparations from tracheal mucus by the indirect fluorescent-antibody procedure. humoral immune response to mg was low, as detected by rapid serum-plate-agglutination, micro-tube-agglutination, hemaggluti ...19846435592
the adhesins of mycoplasma gallisepticum and m. pneumoniae.the growth of m. gallisepticum was monitored in regard to their capacity to haemagglutinate. the maximal potential was with cells grown for about 22 h. m. gallisepticum, like m. pneumoniae, possess a triton shell intracellular filamentous structure which is revealed by exposing the cells to a relatively low concentration of triton-x100. the adhesin of m. gallisepticum was partially purified on sialoglycopeptide conjugated to sepharose-4b. the adhesin fraction was primarily composed of one polype ...19846424524
absence of mycoplasma pneumoniae cytadsorption protein p1 in mycoplasma genitalium and mycoplasma gallisepticum.polyclonal and monoclonal antibodies to mycoplasma pneumoniae protein p1 were nonreactive with whole-cell or soluble preparations of m. genitalium and m. gallisepticum. however, radioimmunoprecipitation performed with hyperimmune rabbit sera raised against each mycoplasma species indicated antigenic cross-reactivity between m. pneumoniae and m. genitalium.19846421736
[usefulness of the agar precipitation test for the determination of the antigenic properties of mycoplasma gallisepticum. i. double diffusion test in agar gel].the usefulness of the double diffusion test in agar gel for the analysis of sero-antigenic structure of mycoplasms was exemplified with serotype s6 mycoplasma gallispeticum. serum of a high precipitation value obtained on rabbits intradermally vaccinated with a suspension of mycoplasms with a complete freund's adjuvant was used. as antigens in the reactions with a specific antiserum was applied an ultrasonic disintegrate of the same suspension of mycoplasms, and in order to elucidate an eventual ...19836415620
mycoplasma gallisepticum isolation in layers.mycoplasma gallisepticum (mg) isolations in live chickens have been made from swabs obtained primarily from the trachea or nasal exudates. as tracheal swabs are often contaminated with feed and because tracheal swabbing may be stressful to the bird, this study was conducted to determine if swabs from the choanal cleft (palatine fissure) would yield mg isolation rates comparable to mg isolation rates of swabs taken from the trachea. commercial leghorns from 17 to 22 weeks of age were inoculated v ...19846387691
a urease-elisa for the detection of mycoplasma infections in elisa utilising a urease-antibody conjugate specific to chicken igg was examined as an alternative to the serum agglutination and the haemagglutination inhibition tests in the diagnosis of mycoplasma gallisepticum and m. synoviae infections in poultry. use of a urease conjugate allowed the serum reactions to be appraised without the need for expensive photometric equipment. non-specific binding of conjugate to antigen was eliminated by treatment of antigen coated microplates with 10% foetal c ...19846378168
improving the mycoplasma gallisepticum and m. synoviae antigen yield by readjusting the ph of the growth medium to the original alkaline state.the ph of mycoplasma gallisepticum (mg) and m. synoviae (ms) growth medium was readjusted back to the original alkaline state when the ph reached 6.1 (for mg) and 6.7 (for ms), and the medium was reincubated until the ph of the medium returned to 6.7 to 6.9. the result was that mg and ms antigen yields were 43 and 54% higher than yields obtained at the usual harvest time.19846372778
application of enzyme-linked immunosorbent assay for detecting antibody to mycoplasma gallisepticum infections in enzyme-linked immunosorbent assay (myco-elisa) was developed to detect antibodies to mycoplasma gallisepticum (mg) in chicken sera. the assay was standardized in terms of optimum antigen concentration, serum dilution, conjugate dilution and incubation temperature, and time. the myco-elisa antigen was prepared from mg whole bacterial cell or its disrupted cell suspension. both preparations showed strong affinity for binding or adsorbing to the surface of polystyrene wells of the microtiter pla ...19836342593
influence of mycoplasma gallisepticum, infectious bronchitis, and cyclophosphamide on chickens protected by native intestinal microflora against salmonella typhimurium or escherichia coli.chickens that have considerable resistance to salmonella typhimurium or escherichia coli infection by early development of a native intestinal microflora shed these bacteria following aerosol exposure to mycoplasma gallisepticum and/or infectious bronchitis virus. administration of cyclophosphamide to similarly treated chickens induced slight shedding of these bacteria, and the combination of cyclophosphamide and respiratory agents magnified the shedding rate. these agents also influenced the is ...19846331365
the effect of oil-emulsion vaccines on the occurrence of nonspecific plate agglutination reactions for mycoplasma gallisepticum and m. synoviae.six groups of ten 18-week-old mycoplasma-free white leghorn pullets were vaccinated with one of the following: mycoplasma gallisepticum (mg) bacterin. haemophilus gallinarum bacterin, pasteurella multocida bacterin, combined infectious bursal disease (ibd)-newcastle (ndv) chicken-embryo-origin (ceo) vaccine. ibd-ndv tissue-culture-origin (tc) vaccine, or saline emulsified in oil; one group received no vaccine. plate agglutination tests for m. synoviae (ms) and mg were done for 10 weeks after vac ...19846331364
further studies on the immunization of chickens with temperature-sensitive mycoplasma gallisepticum mutant.newly hatched chickens were immunized with a temperature-sensitive (ts) mycoplasma gallisepticum (mg) mutant (ts 100). immunized chickens resisted challenge with the virulent s6 strain. the dose of ts mg needed for protection was less than 3.3 x 10(4) colony-forming units. after immunization with ts 100, chickens were subjected to a variety of virus infection and immunosuppressive treatments. neonatal bursectomy or thymectomy, infectious bursal disease virus infection, and infectious bronchitis ...19846326734
avian mycoplasma infections: prototype of mixed infections with mycoplasmas, bacteria and viruses.mixed infections involving mycoplasmas, viruses and bacteria are well recognized in chickens. synergism has been demonstrated between mycoplasma gallisepticum and the viruses of newcastle disease and infectious bronchitis and escherichia coli, although the outcome of infection is influenced by many factors associated with the host and the organisms. airsacculitis in broilers due to m. synoviae or m. gallinarum may be precipitated by concurrent respiratory virus infections including vaccine strai ...19846324630
dna cleavage patterns as indicators of genotypic heterogeneity among strains of acholeplasma and mycoplasma species.electrophoretic patterns of digestion products of acholeplasma and mycoplasma dna by restriction endonucleases were compared. the patterns of acholeplasma axanthum strains isolated from a variety of hosts and habitats differed markedly from each other, indicating considerable genotypic heterogeneity among strains included in this species. heterogeneity was less marked among the acholeplasma oculi strains tested, and was minimal among strains of the avian pathogen mycoplasma gallisepticum. strain ...19836313853
effect of mycoplasma gallisepticum on genetically defined chickens.three parameters were used to measure differences between arkansas progressor and regressor lines of chickens in response to mycoplasma gallisepticum inoculations. the responses of regressor and progressor lines to m. gallisepticum did not differ as judged by antibody response, severity of airsacculitis, and resistance of tracheal ring tissue cultures from 20-day-old progressor and regressor chick embryos to m. gallisepticum.19826284109
pathogenicity of two strains of mycoplasma gallisepticum in broilers.strains f and r of mycoplasma gallisepticum (mg) were compared in two laboratory trials for their relative pathogenicity in terms of inducing airsacculitis and antibody production to mg. chickens exposed to the r strain had significantly higher incidence of air-sac lesions (p less than 0.05) and greater severity of airsacculitis than did chicks exposed to the f strain. in both trials, chickens vaccinated simultaneously with newcastle disease-infectious bronchitis vaccine and exposed to mg had mo ...19806268040
performance of 3 successive generations of specified-pathogenfree chickens maintained as a closed antibodies against salmonella pullorum, mycoplasma gallisepticum, mycoplasma synoviae, haemophilus gallinarum, fowl pox virus, marek's disease virus, herpes virus of turkey, infectious laryngotracheitis virus, avian adenovirus, avian reovirus, infectious bursal disease virus, reticuloendotheliosis virus, avian leukosis virus, avian encephalomyelitis virus and newcastle disease virus were detectable in the sera obtained from these chickens in 3 generations at various ages. antibodies against i ...19806253742
egg production in relation to the results of a long term serological survey of 73 flocks of fowl.seventy-three flocks of fowl were tested at regular intervals for the presence of precipitins to fowl adenovirus (av) and infectious bronchitis virus (ibv), haemaggluinating inhibiting antibodies to bc in 14 virus, and of agglutinins to mycoplasma gallisepticum (m.g.) and mycoplasma synoviae (m.s.). in all the eight flocks affected with egg drop syndrome (eds '76), egg production problems were associated with increasing numbers of bc14 virus reactors and av reactors. in flocks showing production ...19806243424
immunization of chickens with temperature-sensitive mutants of mycoplasma gallisepticum.temperature-sensitive (ts) mutants of the s6 strain of mycoplasma gallisepticum (mg) were used to immunize newly hatched chickens. immunized chickens developed antibodies to the wild-type (wt) s6 strain as demonstrated by serologic tests. mg was recovered from nasal cavities but not from the lower respiratory tract of the immunized chicks. three weeks after intranasal immunization, chickens were challenged via the air sacs with the virulent s6 strain. immunized chickens were significantly better ...19836227317
observations on the pathogenicity of alcaligenes faecalis in chickens.a series of trials was conducted in which specific-pathogen-free (spf) leghorn chicks were exposed to various isolates of alcaligenes faecalis. chicks were inoculated with a. faecalis alone or in combination with newcastle disease/infectious bronchitis (nc/br) vaccine, laryngotracheitis vaccine, infectious bursal disease virus, or mycoplasma gallisepticum. the response was evaluated by morbidity, mortality, airsacculitis, reisolation of a. faecalis, and histopathological lesions of tracheas. alt ...19836227315
capsular material of mycoplasma gallisepticum and its possible relevance to the pathogenic process.a ruthenium red-staining capsule was observed on two pathogenic strains, but not on one nonpathogenic strain of mycoplasma gallisepticum. the capsule appeared to mediate cytadsorption of mycoplasmas to the chicken tracheal epithelium without evidence of membrane fusion. no relationship was seen between the presence of capsule and hemagglutination titers of the strains examined.19826177640
mode of action of the copper(i) complex of 2,9-dimethyl-1,10-phenanthroline on mycoplasma gallisepticum.various physiological important activities of mycoplasma gallisepticum were inhibited by the copper(i) complex of 2,9-dimethyl-1,10-phenanthroline [cu(dmp)2no3]. the energy-yielding metabolism was inhibited because the conversion of pyruvate into lactate was found to be blocked by cu(dmp)2no3, indicating a selective inhibition of lactate dehydrogenase. also, the production rate of acetate and the rate of oxygen uptake by whole cells of m. gallisepticum appeared to be strongly decreased. experime ...19816177282
interaction of mycoplasma gallisepticum with sialyl glycoproteins.the binding of several glycoproteins to freshly grown and harvested cells of mycoplasma gallisepticum was examined. only human glycophorin, the major sialoglycoprotein of the erythrocyte membrane, bound tightly as judged by direct binding assays with 125i-labeled glycoproteins. neuraminidase-treated glycophorin did not bind, suggesting that binding is mediated through sialic acid groups. although other sialoglycoproteins did not appear to bind m. gallisepticum by direct binding assays, some inhi ...19806160107
use of egg yolk in serological tests (elisa and hi) to detect antibody to newcastle disease, infectious bronchitis, and mycoplasma gallisepticum.serum and yolks from commercial flocks and from hens exposed to newcastle disease virus (ndv), infectious bronchitis virus (ibv), and mycoplasma gallisepticum (mg) were tested for immunoglobulin g antibody by the enzyme-linked immunosorbent assay (elisa) and the hemagglutination-inhibition (hi) test. yolks prepared by chloroform extraction and low-speed centrifugation performed well in the serological tests used and were a suitable alternative to serum for antibody determination by the elisa for ...19846098252
volume regulation in mycoplasma gallisepticum.cells of mycoplasma gallisepticum incubated in 250 mm nacl in the absence of glucose for several hours show swelling and eventual lysis. this swelling is believed to be due to colloid osmotic and donnan forces, since it is prevented by the addition of nondiffusable solutes such as sucrose or mgso4. the addition of glucose during the swelling stage (but before lysis) caused shrinkage and return to initial volume. experiments on na+ and h+ movement are consistent with the operation of an atp-drive ...19846096306
serological responses of chickens naturally infected with mycoplasma gallisepticum and the effect of tylosin on these responses. 19676078266
pathology in chickens experimentally inoculated or contact-infected with mycoplasma gallisepticum. 19676073844
transmission of mycoplasma gallisepticum in turkeys. 19676073842
case report: isolation of mycoplasma gallisepticum from bobwhite quail. (colinus virginianus). 19676069465
the action of mycoplasma gallisepticum upon chicken, rabbit, and cow erythrocytes. 19676069464
immunologic response of fowl to mycoplasma gallisepticum and its relationship to latent infection. 19676065939
mycoplasma gallisepticum infection in germfree and conventional chickens: experimental studies with a culture of low virulence. 19676033700
use of formalinized mycoplasma gallisepticum antigens and chicken erythrocytes in hemagglutination and hemagglutination-inhibition studies.antigens were prepared from mycoplasma gallisepticum in the logarithmic phase of growth and were treated with 2, 5, and 10% formalin by slow diffusion through a dialysis sac. chicken erythrocytes were similarly treated with 20% formalin. formalin-treated antigens hemagglutinated fresh and formalinized chicken erythrocytes. the antigens retained this hemagglutinating ability over an extended period.19676029826
a culture medium for the production of mycoplasma gallisepticum antigen. 19676029588
the incidence of mycoplasma gallisepticum, salmonella pullorum, salmonella typhimurium, and newcastle disease virus antibodies in certain wisconsin chickens. 19666008331
the need for standardisation of serological techniques for the detection of mycoplasma gallisepticum infection in poultry. 19666008170
effect of dipping eggs in spiramycin to inactivate mycoplasma gallisepticum. 19666007461
control of chronic respiratory disease. vii. the effect of controlled versus natural infection of chickens with mycoplasma gallisepticum on egg transmission. 19666007130
[on the induction of antibiotic resistance in "mycoplasma gallisepticum"]. 19665999417
airacculitis in turkeys. ii. use of tylosin in the control of the egg transmission of mycoplasma gallisepticum in turkeys. 19665963857
methaemoglobin formation by mycoplasma gallisepticum: the role of hydrogen peroxide. 19665960332
[the influence of the celo virus on artificial infection with mycoplasma gallisepticum]. 19665955161
studies on the chronic respiratory disease of poultry in india. isolation of a pathogenic strain of pleuropneumonia-like-organism (mycoplasma gallisepticum). i. 19665940395
studies of pplo infection. i. the production of cerebral polyarteritis by mycoplasma gallisepticum in turkeys; the neurotoxic property of the mycoplasma.turkey poults injected intravenously with suspensions of mycoplasma gallisepticum develop a fatal neurologic disease associated with polyarteritis affecting almost exclusively the cerebral arteries. the incubation period depends on the dose of organisms. with high doses (10(10) to 10(11) mycoplasmas) the birds become ill and die within a few hours; with lower doses (10(6) to 10(8)) neurologic manifestations appear after 7 days. the rapid onset of neurologic signs after high doses indicates the p ...19665938817
growth of mycoplasma gallisepticum strain j without serum. 19665937705
analysis of the life cycle of mycoplasma gallisepticum.morowitz, harold j. (yale university, new haven, conn.), and jack maniloff. analysis of the life cycle of mycoplasma gallisepticum. j. bacteriol. 91:1638-1644. 1966.-a series of electron microscope observations on mycoplasma gallisepticum strain a5969 have been made by use of thin-section techniques and negative staining. the methods presented a consistent picture of a postdivision cell, which contains a fibrillar nuclear region, surrounding ribosomal region, highly organized bleb at one end of ...19665929782
immunological competence of the chicken embryo and neonatal chicken to mycoplasma gallisepticum. 19665928623
studies of pplo infection. iv. the neurotoxicity of intact mycoplasmas, and their production of toxin in vivo and in vitro.concentrated suspensions of washed mycoplasma neurolyticum produce rolling disease in mice and rats, with neurological manifestations and pathological lesions similar to those seen with the exotoxin of this organism. pretreatment of animals with tetracycline protects completely against the toxic effects of washed suspensions of mycoplasmas, while tetracycline affords no protection against the exotoxin. freeze-thawing disruption of mycoplasma suspensions eliminates their neurotoxicity, while the ...19665925315
the interaction of mycoplasmas with mammalian cells. i. hela cells, neutrophils, and eosinophils.the ultrastructure of three mycoplasma species, mycoplasma pneumoniae, mycoplasma gallisepticum, and mycoplasma neurolyticum, has been studied in isolated form as well as in hela cell cultures and following incubation with human peripheral blood leukocytes. during log growth phase, the organisms could be distinguished from each other on the basis of their fine structure. in mammalian cell cultures, pplo's appeared to proliferate on the plasma membranes which had markedly increased their surface ...19665922745
sialic acid binding sites: role in hemagglutination by mycoplasma gallisepticum.hemagglutination of turkey erythrocytes by mycoplasma gallisepticum was inhibited by mucoproteins containing sialic acid, by sialic acid itself, and by treatment of the erythrocytes with neuraminidase. neuraminidase treatment of the mucoprotein-rich inhibitors reduced or abolished their inhibitory activity. the findings indicate that sialic acid on the erythrocyte surface provides binding sites for mycoplasma gallisepticum.19665903587
microbiologic study of strains of mycoplasma gallisepticum isolated in argentina. 19635888715
dipping of hatching eggs for control of mycoplasma gallisepticum. 19635888714
infectious coryza of chickens. v. influence of mycoplasma gallisepticum infection on chicken infected with haemophilus gallinarum. 19655871448
amelioration of marek's disease by mycoplasma gallisepticum. 19695824203
a strain of mycoplasma gallisepticum previously unreported in this country. 19695818048
serum treatment and antigen dose effects on agglutination and haemagglutination inhibition by mycoplasma gallisepticum antibodies. 19695814057
transmission of mycoplasma gallisepticum, newcastle disease, infectious bronchitis, and combinations in a three-phase broiler house. 19695813240
effects of ph, physical factors, and preservatives on the sensitivity of mycoplasma gallisepticum slide agglutination antigens. 19695812087
factors influencing the growth of mycoplasma gallisepticum. 19695804366
the measurement of antibody to mycoplasma gallisepticum by the metabolic-inhibition technique. 19695793126
antibiotic resistance to a strain of mycoplasma gallisepticum. 19695775620
the evaluation of the metabolic-inhibition technique for the study of mycoplasma gallisepticum. 19695775117
sugar transport in mycoplasma gallisepticum.mycoplasma gallisepticum cells were found to contain two different sugar transport systems, one for d-glucose and alpha-methyl-d-glucoside (alpha-mg) and the other for d-mannose and d-fructose. both systems were noninducible, stereospecific, dependent on temperature and ph, and sensitive to sulfhydryl-blocking reagents. the rate of sugar uptake depended on its external concentration, obeying michaelis-menten kinetics. the sugar accumulated in the cells against a concentration gradient, and an en ...19695773029
eradication of mycoplasma gallisepticum infection in a chicken flock on a breeding farm. 19685753133
methods of eradication of mycoplasma gallisepticum from chickens. 19685751830
latent infection of chickens with mycoplasma gallisepticum. 19685749601
airsacculitis relation to mycoplasmas in turkeys free of mycoplasma gallisepticum. 19685749025
penetration patterns of mycoplasma gallisepticum and newcastle disease virus through the outer structures of chicken eggs. 19685749023
[nutritional and metabolic studies of mycoplasma gallisepticum s6]. 19685725437
[enchancement and inhibtion of growth in mycoplasma gallisepticum by steroids]. 19685707658
non-specific antibodies in chickens inoculated intratracheally with mycoplasma gallisepticum. 19685695834
inactivated culture vaccine against mycoplasma gallisepticum infection in chickens. 19685690690
antibodies for newcastle disease virus and mycoplasma gallisepticum in sera from domestic chickens and game fowl of kenya. 19685690176
early lesions in chicken and turkey embryos inoculated with mycoplasma gallisepticum strain s6 at various stages of incubation. 19685686257
lysis of the limiting membrane of mycoplasma gallisepticum by chemical agents. 19685677978
activity of bottromycin against mycoplasma gallisepticum. 19685673297
[effect of several water-soluble steroids on the growth of mycoplasma gallisepticum]. 19685661579
effect of dextrose in medium for the preparation of mycoplasma gallisepticum plate antigens.antigens for mycoplasma gallisepticum were prepared from organisms propagated in media with and without dextrose supplementation. the antigens made from organisms produced in medium enriched with dextrose were less sensitive than the others in slide agglutination tests.19685647514
serological and bacteriological observation of chickens intranasally inoculated with mycoplasma gallisepticum. 19675587627
mechanism of egg transmission of mycoplasma gallisepticum. 19675582486
multiplication of mycoplasma gallisepticum and newcastle disease virus b1 strain in the respiratory tract of chickens. 19715580498
influence of newcastle disease and infectious bronchitis live vaccines on chickens infected with mycoplasma gallisepticum. 19715569702
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