Publications

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antibiotic resistance to a strain of mycoplasma gallisepticum. 19695775620
the measurement of antibody to mycoplasma gallisepticum by the metabolic-inhibition technique. 19695793126
factors influencing the growth of mycoplasma gallisepticum. 19695804366
effects of ph, physical factors, and preservatives on the sensitivity of mycoplasma gallisepticum slide agglutination antigens. 19695812087
transmission of mycoplasma gallisepticum, newcastle disease, infectious bronchitis, and combinations in a three-phase broiler house. 19695813240
serum treatment and antigen dose effects on agglutination and haemagglutination inhibition by mycoplasma gallisepticum antibodies. 19695814057
a strain of mycoplasma gallisepticum previously unreported in this country. 19695818048
amelioration of marek's disease by mycoplasma gallisepticum. 19695824203
ultrastructure of mycoplasma gallisepticum isolate 1056. 19705494318
a comparison of radiographic lesions in pelvic limbs of chickens infected with mycoplasma gallisepticum, mycoplasma synoviae, or an arthritis-producing virus. 19705503017
immunization against mycoplasma gallisepticum. 19705503019
[attenuation of a strain of mycoplasma gallisepticum for vaccination against avian mycoplasmosis]. 19705534780
[use of the metabolic inhibition test (mi-test) for mycoplasma gallisepticum]. 19705534809
cottonseed oil spray for broiler houses: effects on dust control, mycoplasma gallisepticum spread and broiler performances. 19705537218
the ws7 strain of mycoplasma gallisepticum. 19705492102
electron microscope studies of turkey sinus epithelial cells infected with mycoplasma gallisepticum. 19704917314
serology and gross lesions of turkeys inoculated with an avian influenza a virus, a paramyxovirus, and mycoplasma gallisepticum. 19704314006
pathogenicity of avian reovirus and its influence on the infection of mycoplasma gallisepticum in chickens. 19704318212
mycoplasma synoviae and mycoplasma gallisepticum infections: differentiation by the hemagglutination-inhibition test. 19705440072
the development of a non-infectious carrier state in chickens infected with mycoplasma gallisepticum. 19705448138
mixed infection with mycoplasma gallisepticum and the b1 strain of newcastle disease virus in chickens. 19705453398
contact transmission of mycoplasma gallisepticum and its prevention. 19705453399
non-specific agglutination reactions with mycoplasma gallisepticum antigens. 19705476609
oncolytic effect of a mycoplasma gallisepticum strain on solid ehrlich carcinoma. 19715136326
effect of the newcastle disease virus tcnd strain on mycoplasma gallisepticum infection in chickens. 19715142164
isolation and characterisation of mycoplasma gallisepticum from a fowl. 19715166491
growth and pathogenicity studies of mycoplasma gallisepticum in chicken tracheal organ cultures. 19715167481
the effect of hypotonic solutions on the morphology of cells of mycoplasma gallisepticum. 19714107162
the influence of ph of the culture medium on the sensitivity of mycoplasma gallisepticum antigens for use in certain serological tests.mycoplasma gallisepticum antigens were prepared from organisms cultured in broth medium with glucose. the influence of period of growth, ph of the medium and duration of incubation at low ph (5.0) on the sensitivity of these antigens was determined in certain tests. the most sensitive antigen for the serum plate test was harvested after no more than 8 hr. incubation at ph 5.0. sensitivity in serum plate, haemagglutination and gel diffusion tests was impaired if organisms were incubated at ph 5.0 ...19715002646
the absorption of -globulins to mycoplasma gallisepticum and the possible role in non-specific serological reactions. 19714106502
analysis of the helical ribosome structures of mycoplasma gallisepticum.the helical ribosome structures of mycoplasma gallisepticum have been analyzed by optical diffraction and the rotational symmetry technique. the helix repeat (510 a) is composed of 10 ribosomes in 3 turns. the rotational studies show some ribosome fine structure.19715276300
investigation into rabbit infusion media for the growth of mycoplasma gallisepticum antigens for inoculation into rabbits.serum cholesterol in rabbits was elevated, by intravenous inoculation of 20% tween 80, to amounts thought to have been sufficient to support growth of mycoplasma gallisepticum when 20% of such serum was added to rabbit infusion broth.however, better growth of the organism was obtained with a supplement of 5% rabbit serum and commercial cholesterol. investigation showed that normal rabbit serum may be inhibitory, which would explain these findings. the commercial cholesterol was not antigenic.19715291753
the effect of added energy on induced mycoplasma gallisepticum infection in broilers. 19715550466
growth and survival of germfree and conventional chicks inoculated with mycoplasma gallisepticum. 19715567423
effect of tylosin on chickens infected with mycoplasma gallisepticum and the newcastle disease virus b1 strain. 19715569697
interaction of mycoplasma gallisepticum, mild strains of newcastle disease virus and infectious bronchitis virus in chickens. 19715569701
influence of newcastle disease and infectious bronchitis live vaccines on chickens infected with mycoplasma gallisepticum. 19715569702
multiplication of mycoplasma gallisepticum and newcastle disease virus b1 strain in the respiratory tract of chickens. 19715580498
international reference preparation of anti-mycoplasma gallisepticum serum.the who expert committee on biological standardization requested the central veterinary laboratory, weybridge, england, to arrange a collaborative assay of material they had prepared to determine its suitability to serve as an international reference preparation of anti-mycoplasma gallisepticum serum. fourteen laboratories in eight countries performed comparative assays of the proposed reference preparation together with 3 immune sera from fowls. on the basis of the results obtained, the committ ...19715316619
[studies on mycoplasma gallisepticum by polyacrylamide gel electrophoresis]. 19714942781
pathogenesis of single and mixed infections caused by mycoplasma meleagridis and mycoplasma gallisepticum in turkey embryos. 19715541210
[the occurrence of neuraminidase in mycoplasma gallisepticum]. 19725025772
a new subtype of type a influenzavirus isolated from turkeys.a new subtype of avian influenzavirus a was isolated in january 1967 from an epizootic in a turkey hatchery in ontario, canada. the disease was fatal in 65 of 2 500 hens involved. virus was isolated from lung and trachea tissue of three dead turkeys. sera from convalescent birds contained antibody against the viruses isolated from the outbreak but not against other known type a avian influenzaviruses, newcastle disease virus, myxovirus yucaipa, or mycoplasma gallisepticum.the strain designated a ...19724541004
serum chemistry of chicks maintained at various environmental temperatures and inoculated with newcastle disease and mycoplasma gallisepticum. 19724645729
comparative efficiency of 4 mycoplasma gallisepticum strains as antigens in detecting heterologous infection. 19724648993
neuraminidase activity in mycoplasma gallisepticum.the whole viable mycoplasma gallisepticum (strain tt) organisms were found to possess neuraminidase activity with a ph optimum of 5.8 on substrates such as human transferrin, human alpha(1)-glycoprotein, and rabbit serum. the enzyme operated optimally at ph 4.5 when n-acetylneuraminyl-lactose was used as the test substrate.19724673793
[trial evaluation of a simple screening test for mycoplasma pneumoniae pneumonia by agglutination reaction with mycoplasma gallisepticum antigen]. 19724676769
[effect of gradocycline on mycoplasma gallisepticum in experimentally infected chicks]. 19724554179
enhanced growth of mycoplasma gallisepticum, strain 801. 19724556822
escherichia coli serotypes isolated from chickens infected with mycoplasma gallisepticum. 19724559444
experimental respiratory disease and airsacculitis in fowls caused by mycoplasma gallisepticum. 19725024311
[morphological differentiation of some strains of mycoplasma gallisepticum against different mycoplasma species on solid medium]. 19724145213
ultrastructural characteristics of mycoplasma gallisepticum, m. gallinarum and m. meleagridis. 19725036320
an outbreak of synovitis caused by mycoplasma gallisepticum in chickens. 19725051901
cold hemagglutinin in serum of chicken infected with mycoplasma gallisepticum. 19725051902
removal of non-specific agglutination reactions by avian sera to the serum plate agglutination test for mycoplasma gallisepticum. 19725062152
studies on an outbreak of mycoplasma gallisepticum infection among peafowls (pavo cristatus). 19725072745
membrane association of the deoxyribonucleic acid growing-point region in mycoplasma gallisepticum.newly replicated deoxyribonucleic acid (dna) in mycoplasma gallisepticum a5969 is membrane associated. cells pulse-labeled 1 to 3 min with (3)h-thymidine are lysed by a freeze-thaw procedure. after brief sonic treatment to shear the dna, differential centrifugation gives a cell fraction (p2) that is enriched sevenfold for pulse-labeled dna. p2 contains 80% of the total adenosine triphosphatase activity, 65% of the total cholesterol, and morphologically intact terminal bleb structures. three to f ...19724264449
nuclease activities of mycoplasma gallisepticum as a function of culture age in different media.levels of deoxyribonuclease and ribonuclease activities in the supernatant (soluble plus ribosomes) fraction of mycoplasma gallisepticum were assayed and found to be a function of strain, nutrient, and culture age. in yeast hydrolysate-enriched broth, maximal nuclease activities occurred during exponential growth.19724550663
detection of cholesterol in cell membranes by use of bacterial toxins.a method is described for the detection of cholesterol in membranes from erythrocytes, mycoplasmas, and bacterial cells by a ferritin-labeling technique. membranes treated with cereolysin, a bacterial hemolysin which specifically binds to cholesterol, and then treated with ferritin-antitetanolysin, were specifically ferritin-labeled for cholesterol. a similar antigen-antibody system, streptolysin o-ferritin-antistreptolysin, was also used successfully with erythrocyte membranes. there was an une ...19724623312
studies on the adsorption of certain medium proteins to mycoplasma gallisepticum and their influence on agglutination and haemagglutination reactions.serum proteins from mycoplasma gallisepticum culture medium could be detected on the organisms as a result of incubation at low ph. only certain of the serum proteins, including igg and igm, were found, and the adsorption appears to depress the haemagglutinating activity of the organisms. there was no obvious effect on slide agglutination (sa) sensitivity but an incidental finding was that brief acid treatment enhanced the sa sensitivity of an antigen prepared from a young culture.19724113479
comparison of hemagglutination inhibition and serum plate agglutination tests to detect antibodies to mycoplasma gallisepticum. 19724258551
the rearing and maintenance of breeding chickens in isolators: 1. glass fibre isolators.a method of 'rearing and maintaining chickens from day old to 80 weeks of age free from infectious bronchitis, newcastle disease, infectious avian encephalomyelitis, celo and gal viruses, lymphoid leukosis, marek's disease, mycoplasma gallisepticum, salmonella pullorum and other salmonella spp., staphylococcus aureus, eimeria spp. and helminths is described. the advantages of glass fibre isolators each with its own ventilation system are outlined.197218777370
non-specific agglutination of mycoplasma gallisepticum by rheumatoid factor-like antiglobulin in chickens infected with streptococcus faecalis or staphylococcus aureus. 19734199909
immunity and mortality in chickens infected with mycoplasma gallisepticum: influence of the bursa of fabricius. 19734120327
electron microscopy of ultrathin sections of mycoplasma gallisepticum (strain tt) organisms grown on glass surface. 19734125678
new method for the isolation of membranes from mycoplasma gallisepticum.mycoplasma gallisepticum lysed readily in carbonate bicarbonate buffer at ph 9.2 to 10.5. the hemagglutination titer of the lysates was 2- to 16-fold greater than a cell suspension at the same protein concentration in buffered saline. membranes prepared from cells lysed by this method at ph 10 were relatively free from cytoplasmic contaminants as shown by electron microscopy of thin sections. the membranes retained their hemagglutination activity, gave reactions in immunodiffusion tests identica ...19734200862
tropism of mycoplasma gallisepticum for arterial walls.the fatal encephalopathy associated with m. gallisepticum strain s6 in turkey poults was completely curable by treatment with tylosin or pleuromutilin. the lesions of cerebral polyarteritis disappeared after therapy. polyarthritis developed in some birds after recovery from encephalitis. immunofluorescent studies revealed that intravenously injected organisms became localized as microcolonies within the walls of cerebral and periarticular arteries, and in the glomeruli. turkey igg deposition was ...19734576023
metabolic and growth inhibition of mycoplasma gallisepticum by antiserum. 19734578973
isolation, ultrastructure and antigenicity of mycoplasma gallisepticum membranes.the cell membrane of mycoplasma gallisepticum was isolated by lysing the cells with digitonin. chemical and density-gradient analyses and electron microscopy showed the isolated membranes to be relatively free of cytoplasmic contaminants. the density of the membranes exceeded that of other mycoplasma membranes, indicating a higher protein content. small vesicular extensions seen in the sectioned membranes were interpreted as empty blebs.the isolated membranes, but not the cytoplasmic fraction, e ...19734520511
the use of microtiter hemagglutination-inhibition in mycoplasma gallisepticum testing program. 19734534737
[influence of the composition of the culture media on the growth speed of mycoplasma gallisepticum, s6]. 19734633936
avoidance of non-specific agglutination of mycoplasma gallisepticum by the use of a globulin-free antigen. 19734707485
non-specific agglutination of mycoplasma gallisepticum. 19734730113
the effect of heat killed mycoplasma gallisepticum and m. meleagridis on plasma caeruloplasmin activity in the fowl. 19734792024
effect of ammonia on mycoplasma gallisepticum infection in chickens. 19734792401
interference of mycoplasma gallisepticum with multiplication of newcastle disease virus in chicken tracheal organ cultures. 19734799666
[experimental mycoplasmosis in chickens. ii. pathological findings of chickens inoculated with mycoplasma gallisepticum (strain s6) by the air-sac and intravenous routes]. 19734799960
the virulence of mycoplasma gallisepticum for embryonated fowl eggs. 19734805146
distribution of a phosphoenolypyruvate-dependent sugar phosphotransferase system in mycoplasms.a survey of 10 mycoplasma strains has shown that their capacity to accumulate radioactivity from alpha-methyl-d-glucopyranoside depends on the activity of a phosphoenolpyruvate-dependent phosphotransferase system (pts), and that this system endows the organisms with a high affinity for glucose as a fermentation substrate. pts activity was found in mycoplasma gallisepticum, m. mycoides var. mycoides, and m. mycoides var. capri, but in none of the fermentative acholeplasma strains nor in some of t ...19734688137
deoxyribonucleic acid synthesis in synchronously growing mycoplasma gallisepticum.early log-phase cells of mycoplasma gallisepticum a5969 were synchronized by holding in eagle minimal essential medium (mem) for 2 h. when transferred out of mem into tryptose medium, the cells exhibited synchronous growth. deoxyribonucleic acid (dna) synthesis proceeded continuously during this growth but stopped during the period of cell division. one round of dna replication was observed per cell doubling, and a unique region of dna was found to be permanently bound to the membrane.19734717510
occlusion of brain capillaries by endothelial swelling in mycoplasma infections.occlusion of cerebral capillaries due to acute swelling of the endothelial cells has been demonstrated in the brains of turkeys with encephalopathy caused by mycoplasma gallisepticum (s-6 strain) infection. similar capillary lesions were seen in rats with rolling disease, caused by m. neurolyticum toxin. such lesions may be the basis of ischemic necrosis of the brain.19734514983
comparison of strains of mycoplasma gallisepticum by polyacrylamide gel electrophoresis. 19744592352
partial purification of a membrane-associated deoxyribonucleic acid complex from mycoplasma gallisepticum.a mycoplasma gallisepticum subcellular fraction (p2), which contains the deoxyribonucleic acid replication complex, can be isolated by differential centrifugation of freeze-thaw-lysed cells. the nascent deoxyribonucleic acid is released from p2 by lubrol-wx, sodium dodecyl sulfate, pronase, and deoxyribonuclease, but not by saponin, ribonuclease, phospholipase c, or high-frequency sonic treatment. sonic treatment further fractionates the cell ghost and allows partial purification, on sucrose den ...19744420960
spiramycin- and tylosin-resistant strains of mycoplasma gallisepticum isolated from breeding chickens and pipped eggs. 19744444733
[possible etiological role of mycoplasma gallisepticum in viral hepatitis]. 19744465704
serological comparison of five arginine-utilizing mycoplasma species by two-dimensional immunoelectrophoresis.five arginine-utilizing, nonglycolytic mycoplasma species were compared serologically by two dimensional immunoelectrophoresis. the survey included: mycoplasma hominis, mycoplasma arthritidis, mycoplasma gateae, mycoplasma gallinarum, and three strains of mycoplasma arginini. although the m. arginini strains showed strong cross-reactions, each strain produced a different antigenic profile which was distinct and which indicated significant antigenic differences between strains. the m. arginini st ...19744473427
local immunization in chicken respiratory tract with killed mycoplasma gallisepticum vaccine. 19744474553
effect of temperature on the growth of mycoplasma gallisepticum and on its inhibition by antiserum. 19744615128
an infection in chickens with a strain of mycoplasma gallisepticum of low virulence.a slow-spreading infection due to a strain of mycoplasma gallisepticum was detected serologically in a flock which remained free of clinical symptoms. the strain was shown to be tylosin resistant. serological studies indicated that vertical transmission occurred.19744277360
comparative efficiency of some rapid agglutination antigens for mycoplasma gallisepticum infection.varying compositions of growth media for mycoplasma galli-septicum were used in attempts to produce rapid slide agglutination antigens of high yield and improved specificity. some of the media barely supported growth, others yielded antigen no better than that from conventional media. two media gave good results. one was a conventional medium with horse serum replaced by foetal calf serum. the other was a conventional medium containing 10% horse serum, but at high ph (8.4). when compared with fo ...197418777264
[differentiation of haemophilus strains isolated from chickens. iii. pathogenicity tests in chickens].pathogenicity tests of 6 isolates of the haemophilus-group i (h. paragallinarum) and 8 isolates of the haemophilus-group ii isolated from chickens in the federal german republic and one strain of h. parainfluenzae isolated from man were carried out in 6-week old spf-chickens. infectious coryza (coryza contagiosa gallinarum) could be produced in chickens with all strains of group i after experimental inoculation and by contact exposure. at necropsy 3 weeks post inoculation (p.i.) organisms of gro ...197518777310
[possible synergic action of some drug combinations against mycoplasma gallisepticum].yamamoto and adler's method (1956), making use of the modified medium of edward, was employed to determine the minimal concentrations of gentamicin (gmc), erytrhomycin (erm), oleandomycin (omc) spectinomycin (spm), tetracycline (tc), tylosin (tls), and chloramphenicol (ca), all of them showing bactericidal effects on mycoplasma gallisepticum-s6, applied alone or in combination. in vitro studies showed that relatively most effective were tls, gmc, and spm, when used alone. combining the antibioti ...1975128891
immunization of chickens against mycoplasma gallisepticum infection. 19751092221
a field trial, using killed-mycoplasma gallisepticum vaccine to protect against chicken respiratory mycoplasmosis.in a field trial with chickens, the efficacy of killed mycoplasma gallisepticum vaccine against respiratory mycoplasmosis was determined. broiler chickens were vaccinated by aerosol administration at 3, 14, and 25 days of age, and observations were made of survival rate, body weight gain ratio, macroscopic lesion grade of airsacculitis, isolation of organisms, serologic test results, histopathologic changes, and clinical signs. inhalation of the vaccinal aerosol did not influence livability and ...19751111388
single and mixed infections of avian infectious bronchitis virus and mycoplasma gallisepticum.comparative studies of the clinical signs, pathological changes, multiplication of the pathogens and serological responses were made of groups of chickens infected with either or both avian infectious bronchitis virus and mycoplasma gallisepticum. electron microscopic examination of thin sections of infected tracheas showed that the multiplication of m. gallisepticum was greatly enhanced in the tracheas of chickens which had been previously or simultaneously infected with avian infectious bronch ...19751126565
in vivo efficacy of 81.723 hfu, a new pleuromutilin derivative against experimentally induced airsacculitis in chicks and turkey poults.the efficacy of the pleuromutilin derivative 81.723 hfu was tested in chicks and turkey poults by experimentally infecting them with mycoplasma gallisepticum. the data were treated to obtain the mean effective doses, and these were compared to those obtained with tylosin tartrate. the compounds were administered either by ingluvial catheter or via the drinking water. therapy was started on the day of inoculation or 7 days thereafter, respectively. the experiments showed that the compound 81.723 ...19751147586
hemagglutination-inhibition versus serum plate agglutination in detecting mycoplasma gallisepticum in broiler flocks.sera from 43,040 broilers in 36 flocks were tested for antibodies to mycoplasma gallisepticum by serum plate agglutination (spa) and hemagglutination-inhibition (hi) tests. the importance of testing spa-negative samples for hi antibodies was demonstrated.19751156260
interference of mycoplasma gallisepticum with multiplication of newcastle disease virus in chickens. 19751164323
antimicrobial activities of 81.723 hfu, a new pleuromutilin derivative.the new pleuromutilin derivative 81.723 hfu is extremely active against gram-positive organisms such as streptococci, staphylococci, and against mycoplasmas. a number of shigella, klebsiella, and escherichia coli strains were also found to be quite susceptible to this new agent, whereas other gram-negative organisms like pseudomonas aeruginosa, proteus species, and alcaligenes faecalis proved to be naturally resistant to 81.723 hfu. the new compound acts bacteriostatically. bactericidal effects ...19751170807
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