| pseudoinfection due to tsukamurella paurometabolum. | | 1992 | 1445990 |
| tsukamurella paurometabolum: a novel pathogen causing catheter-related bacteremia in patients with cancer. | tsukamurella paurometabolum is a weakly acid-fast, pleomorphic gram-positive bacterium found in soil. human infection due to this organism has rarely been described, and there are no published accounts of bacteremia. three cases of bacteremia due to t. paurometabolum and related to long-term use of a central venous catheter in patients with cancer who were receiving chemotherapy are described. | 1992 | 1571430 |
| electrophoretic heterogeneity of ribosomal protein at-l30 among actinomycete genera. | the ribosomal proteins from 17 type strains of species belonging to various actinomycete genera were compared by two-dimensional polyacrylamide gel electrophoresis. i detected a striking variability among certain ribosomal proteins (designated at-l30 proteins) with respect to electrophoretic mobility in the first dimension. in contrast, such variability was not observed among ribosomal l30 proteins from other bacteria, such as escherichia coli, bacillus subtilis, pseudomonas aeruginosa, and stap ... | 1992 | 1736962 |
| polyphasic taxonomic study of the genera gordona and tsukamurella including the description of tsukamurella wratislaviensis sp. nov. | representatives of the genera gordona and tsukamurella together with related actinomycetes were the subject of chemotaxonomic, numerical taxonomic and dna homology studies. in the numerical analysis the organisms were examined for 116 unit characters and the data sorted using the simple matching, jaccard and pattern coefficients; clustering was achieved using the unweighted pair group method with arithmetic averages algorithm. the numerical groupings were not affected by the statistics used or b ... | 1991 | 1930572 |
| a phylogenetic analysis of the genus nocardia with 16s rrna gene sequences. | partial sequences of the 16s rrna genes of the type strains of nine species of the genus nocardia were determined following the isolation and cloning of the amplified genes. these sequences were aligned with the sequences of representatives of the genera corynebacterium, gordona, mycobacterium, rhodococcus, and tsukamurella, and phylogenetic trees were inferred by using the fitch-margoliash and neighbor-joining methods. the genus nocardia formed a distinct clade that was most closely associated ... | 1995 | 7537058 |
| phylogenetic analysis of mycolic acid-containing wall-chemotype iv actinomycetes and allied taxa by partial sequencing of ribosomal protein at-l30. | the phylogenetic relationships among 30 mycolic acid-containing wall chemotype iv actinomycete strains and 12 strains belonging to allied taxa were examined by determining the amino acid sequences of the ribosomal at-l30 proteins of these organisms. sequencing 20 n-terminal amino acids of at-l30 preparations revealed that the members of the genera containing mycolic acid-containing actinomycetes form two clusters; the first cluster contains the genera nocardia, rhodococcus, gordona, and tsukamur ... | 1995 | 7547284 |
| persistent bacteremia due to tsukamurella paurometabolum in a patient undergoing hemodialysis: case report and review. | | 1994 | 8075284 |
| a cancer patient with central venous catheter-related sepsis caused by tsukamurella paurometabolum (gordona aurantiaca) | | 1993 | 8399886 |
| comparison of 16s ribosomal rna genes in clavibacter michiganensis subspecies with other coryneform bacteria. | nearly complete sequences (97-99%) of the 16s rrna genes were determined for type strains of clavibacter michiganensis subsp. michiganensis, clavibacter michiganensis subsp. insidiosus, clavibacter michiganensis subsp. sepedonicus, and clavibacter michiganensis subsp. nebraskensis. the four subspecies had less than 1% dissimilarity in their 16s rrna genes. comparative studies indicated that the c. michiganensis subsp. shared relatively high homology with the 16s rrna gene of clavibacter xyli. fu ... | 1995 | 8590406 |
| rapid growth and increased biomass yield of mycobacterium farcinogenes and some related taxa in broth and agar media. | a broth medium containing yeast extract (4 g/l), glucose (15 g/l), magnesium sulphate (0.5 g/l), tri-sodium citrate (1.5 g/l), potassium sulphate (0.5 g/l), ammonium ferric citrate (trace) and buffered with potassium di-hydrogen phosphate (5 g/l) was formulated and found to support a luxuriant growth of mycobacterium farcinogenes strains that was superior to the conventional media used before. similar results were obtained with m. senegalense strains and with representatives of the genera gordon ... | 1995 | 8594852 |
| [tsukamurella infections. review of the literature apropos of a case]. | the genus tsukamurella belongs to the family nocardiaceae, and is an environmental saprophyte. the type species is tsukamurella paurometabola. its microbiological identification and differentiation from the other species containing mycolic acids can be difficult. there has been a few cases of human infections reported, usually in patients with special conditions, such as chronic lung pathology, immuno-suppression (leukemia, solid tumors, maybe hiv-infection) or the long-term use of indwelling ca ... | 1997 | 9097849 |
| rapid identification of clinically significant species and taxa of aerobic actinomycetes, including actinomadura, gordona, nocardia, rhodococcus, streptomyces, and tsukamurella isolates, by dna amplification and restriction endonuclease analysis. | a previously described pcr-restriction fragment length polymorphism (rflp) identification schema for nocardia that used an amplified 439-bp segment (amplicon) of the 65-kda heat shock protein gene was evaluated for potential use with isolates of all clinically significant aerobic actinomycetes. the study included 28 reference (american type culture collection) strains and 198 clinical isolates belonging to 20 taxonomic groups. of these 198 isolates, 188 could be differentiated by this pcr-rflp m ... | 1997 | 9157134 |
| tsukamurella inchonensis bacteremia in a patient who ingested hydrochloric acid. | | 1997 | 9195101 |
| identification of rhodococcus, gordona and dietzia species using carbon source utilization tests ("biotype-100" strips). | the "biotype-100" identification system (biomérieux, la balme-ies-grottes, france) based on carbon source utilization was evaluated for its ability to discriminate among 10 species of rhodococcus, 7 species of gordona and one species of dietzia. the type strains of three species of tsukamurella and 8 species of nocardia were also included in the study. results were compared with chemotaxonomic and conventional data. carbon source utilization was shown to be reliable, rapid and easy to use when c ... | 1997 | 9765864 |
| rapid identification of clinically relevant nocardia species to genus level by 16s rrna gene pcr. | two regions of the gene coding for 16s rrna in nocardia species were selected as genus-specific primer sequences for a pcr assay. the pcr protocol was tested with 60 strains of clinically relevant nocardia isolates and type strains. it gave positive results for all strains tested. conversely, the pcr assay was negative for all tested species belonging to the most closely related genera, including dietzia, gordona, mycobacterium, rhodococcus, streptomyces, and tsukamurella. besides, unlike the la ... | 1999 | 9854071 |
| production and structure elucidation of di- and oligosaccharide lipids (biosurfactants) from tsukamurella sp. nov. | the bacterium tsukamurella sp. nov., isolated from soil, was found to produce novel glycolipids when grown on sunflower oil as the sole carbon source. the glycolipids were isolated by chromatography on silica columns and their structures elucidated using a combination of multidimensional nmr and ms techniques. the three main components are 2,3-di-o-acyl-alpha-d-glucopyranosyl-(1-1)-alpha-d-glucopyranose, 2,3-di-o-acyl-beta-d-glucopyranosyl-(1-2)-4,6-di-o-acyl-alpha-d- glucopyranosyl-(1-1)-alpha- ... | 1998 | 9866171 |
| tsukamurella strandjordae sp. nov., a proposed new species causing sepsis. | we have isolated a gram-positive, weakly acid-alcohol-fast, irregular rod-shaped bacterium from cultures of blood from a 5-year-old girl with acute myelogenous leukemia. this isolate was compared with 14 other strains including reference strains of tsukamurella species by a polyphasic approach based on physiological and biochemical properties, whole-cell short-chain fatty acid and mycolic acid analyses, dna-dna hybridization, and sequencing of the 16s rrna gene. this isolate represents a new tax ... | 2001 | 11283073 |
| central venous catheter-related bacteremia due to tsukamurella species in the immunocompromised host: a case series and review of the literature. | we report 6 cases of bacteremia due to tsukamurella species, all of which were in immunosuppressed patients with indwelling central venous catheters (cvcs). fewer than 20 cases of serious illness due to these gram-positive bacilli have been reported in the medical literature; these cases have mostly been ascribed to the species tsukamurella paurometabola. tsukamurella species are frequently misidentified as rhodococcus or corynebacterium species. we used high-performance liquid chromatography to ... | 2002 | 12228839 |
| neuropeptides in the livers of mice during bacterial infections. | neuropeptides such as substance p (sp) and vasoactive intestinal peptide (vip) are known to act as immunomodulators. we investigated the induction of sp and vip in the livers of mice infected with listeria monocytogenes or injected with tsukamurella paurometabolum. vip was detected in the livers of mice after l. monocytogenes infection by an immunohistochemical technique and preprovip mrna, which was detected by reverse transcription-polymerase chain reaction (pcr), was induced post infection. h ... | 1998 | 9544784 |
| bacterial degradation of styrene in waste gases using a peat filter. | a biofiltration process was developed for styrene-containing off-gases using peat as filter material. the average styrene reduction ratio after 190 days of operation was 70% (max. 98%) and the mean styrene elimination capacity was 12 g m-3 h-1 (max. 30 g m-3 h-1). efficient styrene degradation required addition of nutrients to the peat, adjustment of the ph to a neutral level and efficient control of the humidity. maintenance of the water balance was easier in a down-flow than in an up-flow proc ... | 1997 | 9457801 |
| tsukamurella tyrosinosolvens sp. nov. | chemotaxonomic and 16s ribosomal dna sequence analyses of four bacterial isolates from blood cultures from patients with cardiac pacemaker implants and sputa of patients with chronic lung infections clearly demonstrated that these bacteria belong to the genus tsukamurella. dna-dna hybridization data, as well as the physiological characteristics of the isolates, indicate that they are closely related and belong to a single species that differs from previously described members of the genus tsukam ... | 1997 | 9226890 |
| tsukamurella paurometabola lipoglycan, a new lipoarabinomannan variant with pro-inflammatory activity. | the genus tsukamurella is a member of the phylogenetic group nocardioform actinomycetes and is closely related to the genus mycobacterium. the mycobacterial cell envelope contains lipoglycans, and of particular interest is lipoarabinomannan, one of the most potent mycobacterial immunomodulatory molecules. we have investigated the presence of lipoglycans in tsukamurella paurometabola and report here the isolation and structural characterization of a new lipoarabinomannan variant, designated tpala ... | 2004 | 15031299 |
| a proposal to reclassify nocardia pinensis blackall et al. as skermania piniformis gen. nov., comb. nov. | the type strain of nocardia pinensis was the subject of chemotaxonomic and 16s ribosomal dna sequencing studies. the resultant nucleotide sequence was aligned with the sequences of representatives of the genera corynebacterium, dietzia, gordona, mycobacterium, nocardia, rhodococcus, and tsukamurella, and phylogenetic trees were generated by using the fitch-margoliash, maximum-parsimony, maximum-likelihood, and neighbor-joining methods. it was evident from the phylogenetic analyses that n. pinens ... | 1997 | 8995814 |
| sequence-based identification of aerobic actinomycetes. | we investigated the utility of 500-bp 16s rrna gene sequencing for identifying clinically significant species of aerobic actinomycetes. a total of 28 reference strains and 71 clinical isolates that included members of the genera streptomyces, gordonia, and tsukamurella and 10 taxa of nocardia were studied. methods of nonsequencing analyses included growth and biochemical analysis, pcr-restriction enzyme analysis of the 439-bp telenti fragment of the 65 hsp gene, susceptibility testing, and, for ... | 2004 | 15184431 |
| cavitary pneumonia secondary to tsukamurella in an aids patient. first case and a review of the literature. | tsukamurella is a gram-positive, variable rod-shaped, weakly acid-alcohol-fast, non motile, aerobic bacterium that belongs to the genus rhodococcus. tsukamurella has been reported as a cause of infections in humans with immunosuppression and indwelling foreign bodies. it has also been isolated in one patient with aids (acquired immunodeficiency syndrome) as a saprophytic organism. optimal management of infections secondary to this micro-organism is still uncertain due to the paucity of cases. th ... | 2004 | 15194243 |
| tsukamurella pulmonis sp. nov. | chemotaxonomic and 16s ribosomal dna sequence analyses of an isolate from the sputum of a patient with a mycobacterial lung infection clearly delineated a new species of the genus tsukamurella. this new species can be defined on the basis of genotypic and phenotypic data. the name tsukamurella pulmonis sp. nov. is proposed for this organism; the type strain is immib d-1321t (= dsm 44142t). this isolate shows 44.2 and 36.2% dna relatedness to tsukamurella paurometabola dsm 20162t (t = type strain ... | 1996 | 8934902 |
| cutaneous infection caused by tsukamurella paurometabolum. | | 1996 | 8909862 |
| different rifampicin inactivation mechanisms in nocardia and related taxa. | mycolic acid-containing bacteria inactivate rifampicin in a variety of ways such as glucosylation, ribosylation, phosphorylation and decolorization. these inactivations were found to be a species-specific phenomena in nocardia and related taxa. gordona, tsukamurella and fast-growing mycobacterium modified rifampicin by ribosylation of the 23-oh group of the antibiotic. such ribosylation was not observed in rhodococcus and corynebacterium, but phosphorylation of the 21-oh group of rifampicin was ... | 1996 | 8871521 |
| tsukamurella and hiv infection. | | 1995 | 8605061 |
| a simple chemical test to distinguish mycobacteria from other mycolic-acid-containing actinomycetes. | two hundred and fifty-two representatives of the general corynebacterium, gordona, mycobacterium, nocardia, rhodococcus and tsukamurella were degraded by alkaline hydrolysis and their mycolic acids extracted as methyl esters following phase-transfer-catalysed esterification. when the mycolic acid methyl esters were treated with a mixture of acetonitrile and toluene all mycobacterial mycolates formed copious white precipitates whereas all but 5 out of the 106 non-mycobacterial mycolates remained ... | 1993 | 8245845 |
| rapid identification of mycolic acid patterns of mycobacteria by high-performance liquid chromatography using pattern recognition software and a mycobacterium library. | current methods for identifying mycobacteria by high-performance liquid chromatography (hplc) require a visual assessment of the generated chromatographic data, which often involves time-consuming hand calculations and the use of flow charts. our laboratory has developed a personal computer-based file containing patterns of mycolic acids detected in 45 species of mycobacterium, including both slowly and rapidly growing species, as well as tsukamurella paurometabolum and members of the genera cor ... | 1994 | 8195387 |
| central line-related bacteraemia due to tsukamurella tyrosinosolvens in a haematology patient. | | 2005 | 16022132 |
| permeabilization of mycolic-acid-containing actinomycetes for in situ hybridization with fluorescently labelled oligonucleotide probes. | the application of whole-cell hybridization using labelled oligonucleotide probes in microbial systematics and ecology is limited by difficulties in permeabilizing many gram-positive organisms. in this investigation paraformaldehyde treatment, acid methanolysis and acid hydrolysis were evaluated as a means of permeabilizing mycolic-acid-containing actinomycetes prior to hybridization with a fluorescently labelled oligonucleotide probe designed to bind to a conserved sequence of bacterial 16s rrn ... | 1994 | 8000549 |
| the potential misidentification of tsukamurella pulmonis as an atypical mycobacterium species: a cautionary tale. | | 2006 | 16533999 |
| diversity of actinomycetes isolated from challenger deep sediment (10,898 m) from the mariana trench. | thirty-eight actinomycetes were isolated from sediment collected from the mariana trench (10,898 m) using marine agar and media selective for actinomycetes, notably raffinose-histidine agar. the isolates were assigned to the class actinobacteria using primers specific for members of this taxon. the phylogenetic analysis based on 16s rrna gene sequencing showed that the isolates belonged to the genera dermacoccus, kocuria, micromonospora, streptomyces, tsukamurella and williamsia. all of the isol ... | 2006 | 16538400 |
| analysis of 16s-23s intergenic spacer regions of the rrna operons in tsukamurella pulmonis. | | 2006 | 16613139 |
| [tsukamurella tyrosinosolvens cultured from sputum of a patient who received total gastrectomy for gastric cancer]. | a 79-year old woman underwent total gastrectomy under the diagnosis of gastric cancer in feb. 2003. in the beginning of jan. 2005, she noticed hemosputum and was admitted to our hospital. chest radiograph and ct disclosed bilateral upper lobe-dominant nodular opacities in the subpleural areas and ground-glass opacities in right s6. transbronchial lung biopsy was performed, but no useful information for the diagnosis was obtained. ziehl-neelsen stain was negative for the smear of the sputum at ad ... | 2006 | 16910601 |
| dietzia, a new genus including dietzia maris comb. nov., formerly rhodococcus maris. | sequencing of the 16s ribosomal dnas (rdna) of two strains of rhodococcus maris was performed to determine the relationship of this species to other mycolic acid-containing actinomycetes. for this purpose we also determined the 16s rdna sequences for the type species of the genus rhodococcus, rhodococcus rhodochrous, and for mycobacterium chlorophenolicum (formerly rhodococcus chlorophenolicus), rhodococcus erythropolis, gordona bronchialis, and gordona terrae, for which only partial sequence da ... | 1995 | 7857805 |
| phylogeny of the genus corynebacterium deduced from analyses of small-subunit ribosomal dna sequences. | we determined almost complete small-subunit ribosomal dna sequences of 50 reference strains belonging to the genera corynebacterium, rhodococcus, and gordona and compared these sequences with previously published sequences. three phylogenetic methods (the neighbor-joining, maximum-likelihood, and maximum-parsimony methods), as well as a bootstrap analysis, were used to assess the robustness of each topology which we obtained. the results of comparative phylogenetic analyses confirmed that the ge ... | 1995 | 7547293 |
| phylogenetic analysis of the genus corynebacterium based on 16s rrna gene sequences. | the 16s rrna gene sequences of 30 strains representing 23 validated corynebacterium species and 7 currently non-valid corynebacterium species were determined. these sequences were aligned with the sequences of other corynebacterium species and related actinomycete taxa. a comparative sequence analysis revealed that there is considerable phylogenetic depth and internal structure in the genus corynebacterium. turicella otitidis and the amycolate species corynebacterium amycolatum were located at t ... | 1995 | 7547291 |
| coexistence of primary adenocarcinoma of the lung and tsukamurella infection: a case report and review of the literature. | a major diagnostic challenge in the evaluation of a cavitary lung lesion is to distinguish between infectious and malignant etiologies. | 2008 | 18554413 |
| a phylogeny of the genus nocardia deduced from the analysis of small-subunit ribosomal dna sequences, including transfer of nocardia amarae to the genus gordona as gordona amarae comb. nov. | according to phylogenetic analyses of nearly complete small-subunit ribosomal dna sequences, the genus nocardia should not comprise the two species nocardia petroleophila and nocardia amarae. n. amarae should be reassigned to the genus gordona as gordona amarae. all of the other nocardia species form a monophyletic unit, closely related to species of the genus rhodococcus. it is proposed to revive the name 'cmn' to comprise the genera corynebacterium, tsukamurella, mycobacterium, gordona, rhodoc ... | 1994 | 7545965 |
| characterization of the microbial community involved in the suppression of pythium aphanidermatum in cucumber grown on rockwool. | abstract the root pathogen pythium aphanidermatum induced lower levels of disease in cucumber (cucumis sativus) plants on unsterilized, re-used rockwool slabs than on heat-sterilized, re-used rockwool. several recolonization treatments of the sterilized rockwool enhanced the suppressiveness of the rockwool. microbial community structures in the different rockwool treatments were investigated by plate counts on selective media. disease suppressiveness in the different rockwool treatments showed t ... | 2005 | 18943014 |
| outbreak of pseudoinfection with tsukamurella paurometabolum traced to laboratory contamination: efficacy of joint epidemiological and laboratory investigation. | from january 1988 to may 1989, one hospital in south carolina reported 12 isolates of tsukamurella paurometabolum from 10 patients. there were no common risk factors among the patients. case-control studies revealed that the positive specimens were significantly more likely to have been processed in the tb/fungal room, to have been tissue samples, and to have been handled by one technician. typing on the basis of biochemical, antimicrobial resistance, southern blot, and ribotype profiles showed ... | 1992 | 1318084 |
| [a case of catheter-related bacteremia of tsukamurella pulmonis]. | tsukamurella pulmonis is an aerobic actinomycete. we report a catheter-related bacteremia of t. pulmonis. a 39 yr-old male with all was hospitalized to receive bone marrow transplantation (bmt). although the patient developed a high fever at the 7th hospital day (hd), it subsided with vancomycin treatment, and he received bmt at 9th hd. fever resurged at 16th hd despite sustained treatment with vancomycin, meropenem, and amphotericin b, but subsided with removal of hickman catheter (hc) at 19th ... | 2009 | 19262077 |
| anhydrobiosis quotient: a novel approach to evaluate stability in desiccated bacterial cells. | the major objective of this study was the development of a methodology to quantify the anhydrobiotic ability of bacteria and its application to evaluate the stability of desiccated bacterial cells using the biocontrol agent tsukamurella paurometabola c-924 as a model of anhydrobiote. | 2009 | 19291234 |
| [aerobic actinomycetes and their significance in human and animal pathology. i. actinomycetales infections--characteristics, etiology, epidemiology and pathogenesis]. | aerobic actinomycetes causing nocardiosis, grt-(gordona, rhodococcus, tsukamurella) infections, actinomycetomas, dermatophilosis and extrinsic allergic alveolitis, including etiology, epidemiology and pathogenesis of the diseases are described. the name "actinomycetosis" for all these diseases has been proposed. many clinical important and potential pathogens, as well as an increasing number of opportunistic, immunosuppressed and immunocompromised cases are discussed. a review of the abundant li ... | 1992 | 1293579 |
| tsukamurella tyrosinosolvens intravascular catheter infection identified using 16s ribosomal dna sequencing. | cultures of blood from a hemodialysis line repeatedly yielded a gram-positive rod. the organism was identified as tsukamurella tyrosinosolvens by 16s ribosomal dna sequencing, and the patient was treated successfully by removal of the line. | 2003 | 12594657 |
| fighting foam with phages? | seventeen (17) phages infective for the mycolata were isolated from six samples of activated sludge using 21 prospective hosts from the genera dietzia, gordonia, nocardia, rhodococcus, tsukamurella and mycobacterium. their morphology indicated that they were all members of the viral family siphoviridae, but they varied in the size of the icosahedral head and length of non-contractile tail, suggesting they were different. this was confirmed by host-range studies with 47 strains of mycolata, which ... | 2002 | 12216679 |
| transfer of tsukamurella wratislaviensis goodfellow et a. 1995 to the genus rhodococcus as rhodococcus wratislaviensis comb. nov.. | a polyphasic study was undertaken to clarify the taxonomic position of the type strain (n805t) of tsukamurella wratislaviensis. this organism showed a combination of phenotypic properties, notably chemotaxonomic markers, consistent with its classification in the genus rhodococcus. comparative 16s rdna sequencing studies indicated that strain 805t falls into the rhodococcus erythropolis subclade, where it forms a monophyletic group with the type strains of rhodococcus opacus and rhodococcus perco ... | 2002 | 12054234 |
| catheter-related bacteremia due to tsukamurella pulmonis. | | 1998 | 11864235 |
| alkanesulfonate degradation by novel strains of achromobacter xylosoxidans, tsukamurella wratislaviensis and rhodococcus sp., and evidence for an ethanesulfonate monooxygenase in a. xylosoxidans strain ae4. | novel isolates of achromobacter xylosoxidans, tsukamurella wratislaviensis and a rhodococcus sp. are described. these grew with short-chain alkanesulfonates as their sole source of carbon and energy. t. wratislaviensis strain sb2 grew well with c(3)-c(6) linear alkanesulfonates, isethionate and taurine, rhodococcus sp. strain cb1 used c(3)-c(10) linear alkanesulfonates, taurine and cysteate, but neither strain grew with ethanesulfonate. in contrast, a. xylosoxidans strain ae4 grew well with etha ... | 2001 | 11734883 |
| tsukamurella peritonitis associated with continuous ambulatory peritoneal dialysis. | a case of tsukamurella peritonitis associated with peritoneal dialysis in a 23-year-old woman is described. the organism was difficult to identify and was mistaken for corynebacterium and atypical mycobacteria. despite prolonged, multidrug, antimicrobial therapy with conventional antibiotics including vancomycin, ciprofloxacin, rifampin, gentamicin and ceftazidime, catheter removal was required to successfully treat peritonitis. human infection due to this organism is rare and has been previousl ... | 2001 | 11597040 |
| tsukamurella tyrosinosolvens--an unusual case report of bacteremic pneumonia after lung transplantation. | lung transplant recipients have an increased risk for actinomycetales infection secondary to immunosuppressive regimen. | 2009 | 19909497 |
| tsukamurella: an unrecognized mimic of atypical mycobacterial keratitis? the first case report. | the purpose of this study was to report on tsukamurella as a mimic of atypical mycobacterial infection. | 2010 | 20098312 |
| bacteria that masquerade as fungi: actinomycosis/nocardia. | the order actinomycetales includes phylogenetically diverse but morphologically similar aerobic and anaerobic bacteria that exhibit filamentous branching structures which fragment into bacillary or coccoid forms. the aerobic actinomyces are a large, diverse group of gram-positive bacteria including nocardia, gordona, tsukamurella, streptomyces, rhodococcus, streptomycetes, mycobacteria, and corynebacteria. the anaerobic genera of medical importance include actinomyces, arachnia, rothia, and bifi ... | 2010 | 20463251 |
| immunotherapy for the prevention of myointimal hyperplasia after experimental balloon injury of the rat carotid artery. | we assessed the effect of novel immunotherapeutic heat-killed bacterial (actinomycetales) preparations on the development of myointimal hyperplasia (mih) in a rat carotid balloon trauma model and the effect on the immune response by measuring the expression of interferon gamma (ifn-gamma; (th1) and interleukin 4 (il-4; th2). there was a significant reduction (p < .001) in intima/media ratios (mean +/- sem) in the rats treated by immunomodulation (0.52 +/- 0.03 gordonia bronchialis, 0.60 +/- 0.03 ... | 2010 | 20498142 |
| [filamentous "contaminants" in the mycobacteriology laboratory; their culture, identification and clinical significance]. | frequent "contaminants" detected during mycobacterial culture of decontaminated samples are bacteria of the order actinomycetales. these are usually bacteria classified as the family corynebacterineae, genera corynebacterium, dietzia, gordonia, nocardia, rhodococcus and tsukamurella. these bacteria frequently colonize the airways and, under certain circumstances, they may cause life-threatening diseases. in severely immunocompromised patients, they regularly cause life-threatening infections wit ... | 2010 | 20503156 |
| infection of a knee prosthesis with tsukamurella species. | a 69-year-old woman with a history of multiple infections of a postoperative wound from a knee replacement was diagnosed with an infection with tsukamurella sp. the infection was treated with a course of vancomycin and pipercillin/tazobactam, followed by a course of clarithromycin, ciprofloxacin, and ethambutol. the patient responded well. this represents the first report of a tsukamurella infection of an artificial joint. | 1999 | 10456728 |
| selected microbial glycolipids: production, modification and characterization. | this chapter deals with two types of biosurfactants that are not in the spotlight of general research: glycoglycerolipids and oligosaccharide lipids. the main focus is on glycolglycerolipids from marine bacteria like microbacterium spec. dsm 12583, micrococcus luteus (hel 12/2) and bacillus pumilus strain aas3 and on oligosaccharide lipids from tsukamurella spec. dsm 44370 and nocardia corynebacteroides sm1. general and special structures, microbial producers, production conditions and chemo-enz ... | 2010 | 20545283 |
| tsukamurella conjunctivitis: a novel clinical syndrome. | in this report, we describe the first three cases of tsukamurella conjunctivitis in the literature. all three patients presented with congestion of one eye with small amounts of serous discharge for 1 to 2 days. all three recovered after 10 days of treatment with polymyxin b-neomycin or chloramphenicol eyedrops. sequencing of the 16s rrna genes of the three isolates recovered from the serous discharge of the three patients showed that they were all tsukamurella species. the phenotypic characteri ... | 2003 | 12843095 |
| tsukamurella tyrosinosolvens and rhizobium radiobacter sepsis presenting with septic pulmonary emboli. | clin microbiol infect abstract: septic pulmonary embolism (spe) is an uncommon, but life-threatening event that is usually associated with extrapulmonary infections. we report the first case of bilateral spe secondary to a central venous catheter-related bloodstream infection involving pathogens commonly considered environmental contaminants: tsukamurella tyrosinosolvens and rhizobium radiobacter. empirical levofloxacin treatment was confirmed by in vitro susceptibility data and produced prompt ... | 2010 | 20946410 |
| mycolic acid-containing bacteria induce natural-product biosynthesis in streptomyces species. | natural products produced by microorganisms are important starting compounds for drug discovery. secondary metabolites, including antibiotics, have been isolated from different streptomyces species. the production of these metabolites depends on the culture conditions. therefore, the development of a new culture method can facilitate the discovery of new natural products. here, we show that mycolic acid-containing bacteria can influence the biosynthesis of cryptic natural products in streptomyce ... | 2010 | 21097597 |
| tsukamurella spumae sp. nov., a novel actinomycete associated with foaming in activated sludge plants. | a polyphasic taxonomic study was undertaken to establish the taxonomic position of six representative strains isolated from activated sewage sludge foam. the organisms were found to have chemical and morphological properties consistent with their assignment to the genus tsukamurella. dna:dna relatedness studies showed that five out of the six isolates formed a distinct genomic species, the remaining strain was most closely associated with this taxon. the five isolates had a unique phenotypic pro ... | 2003 | 14529179 |
| improved identification of gordonia, rhodococcus and tsukamurella species by 5'-end 16s rrna gene sequencing. | the identification of fastidious aerobic actinomycetes such as gordonia, rhodococcus, and tsukamurella has remained a challenge leading to clinically significant misclassifications. this study is intended to examine the feasibility of partial 5'-end 16s rrna gene sequencing for the identification of gordonia, rhodococcus, and tsukamurella, and defined potential reference sequences for species from each of these genera. | 2011 | 21240067 |
| potentially pathogenic features of heterotrophic plate count bacteria isolated from treated and untreated drinking water. | heterotrophic plate counts (hpcs) are commonly used to assess the general microbiological quality of drinking water. drinking water quality specifications worldwide recommend hpc limits from 100 to 500 cfu ml(-1). a number of recent studies revealed evidence that these bacteria may not be as harmless as generally accepted. it appears that immuno-compromised individuals are particularly at risk. this would include the very young and very old patients with diseases such as aids and patients on the ... | 2004 | 15145586 |
| tsukamurella pseudospumae sp. nov., a novel actinomycete isolated from activated sludge foam. | the taxonomic position of two tsukamurella strains isolated from activated sludge foam was clarified. the organisms, isolates jc85 and n1176t, were found to have chemical and morphological properties typical of members of the genus tsukamurella. dna-dna relatedness studies showed that the strains formed a distinct genomic species that was most closely related to tsukamurella spumae. the two isolates also share a range of phenotypic properties that distinguishes them from representatives of all s ... | 2004 | 15280293 |
| identification of a cation-specific channel (tipa) in the cell wall of the gram-positive mycolata tsukamurella inchonensis: the gene of the channel-forming protein is identical to mspa of mycobacterium smegmatis and mppa of mycobacterium phlei. | detergent extracts of whole cells of the gram-positive bacterium tsukamurella inchonensis atcc 700082, which belongs to the mycolata, were studied for the presence of ion-permeable channels using lipid bilayer experiments. one channel with a conductance of about 4.5 ns in 1 m kcl was identified in the extracts. the channel-forming protein was purified to homogeneity by preparative sds-page. the protein responsible for channel-forming activity had an apparent molecular mass of about 33 kda as jud ... | 2004 | 15533305 |
| implantable cardioverter-defibrillator infection caused by tsukamurella. | human infections with tsukamurella are very rare with only 13 reported cases in the literature. certain conditions, such as immunosuppression, an indwelling foreign body, and postoperative wounds predispose humans to tsukamurella infections. the rarity of tsukamurella infection in humans makes its diagnosis and treatment very difficult. this article describes the first case of implantable cardioverter-defibrillator (icd) infection related to tsukamurella in the literature. | 2004 | 15617463 |
| clinical impact of the use of 16s rrna sequencing method for the identification of "difficult-to-identify" bacteria in immunocompromised hosts. | molecular method of 16s rrna sequencing is reported to be helpful in the accurate identification of organisms with ambiguous phenotypic profiles. we analyzed the use of 16s rrna sequencing method to identify clinically significant, "difficult-to-identify" bacteria recovered from clinical specimens, and evaluated its role in patient management and consequent clinical outcome. among the 172 "difficult-to-identify" bacteria recovered over a 4-year period, 140 were gram-positive cocci or gram-negati ... | 2011 | 22093075 |
| structural elucidation of the predominant motifs of the major cell wall arabinogalactan antigens from the borderline species tsukamurella paurometabolum and mycobacterium fallax. | tsukamurella paurometabolum and mycobacterium fallax are members of the suprageneric actinomycete group corynebacterineae that possesses a cell wall skeleton composed of a peptidoglycan to which an arabinogalactan is covalently attached. this polysaccharide is further modified by esterification with c60-c80 mycolic acid residues in mycobacteria and t. paurometabolum. however, m. fallax and t. paurometabolum produce polyenoic (up to six double bonds) mycolic acids whereas the most common type of ... | 2005 | 15761023 |
| tsukamurella tyrosinosolvens intravascular catheter-related bacteremia in a haematology patient: a case report. | tsukamurella spp. are a rare but important cause of intravascular catheter-related bacteremia in immunocompromised patients. the organism is an aerobic, gram-positive, weakly acid-fast bacillus that is difficult to differentiate using standard laboratory methods from other aerobic actinomycetales such as nocardia spp., rhododoccus spp., gordonia spp., and the rapid growing mycobacterium spp. we report a case of tsukamurella tyrosinosolvens catheter-related bacteremia in a 51-year-old haematology ... | 2011 | 22195371 |
| reclassification of nocardia corynebacterioides serrano et al. 1972 (approved lists 1980) as rhodococcus corynebacterioides comb. nov. | the type strain of nocardia corynebacterioides was the subject of a polyphasic taxonomic study. the 16s rrna gene sequence was aligned with the sequences of representatives of the genera corynebacterium, dietzia, gordonia, mycobacterium, nocardia, rhodococcus, skermania, tsukamurella and williamsia, and phylogenetic trees were constructed by using maximum-parsimony, maximum-likelihood and neighbour-joining methods. it was evident from the phylogenetic analysis that n. corynebacterioides represen ... | 2005 | 15879280 |
| tsukamurella inchonensis sp. nov. | chemotaxonomic and genomic 16s ribosomal dna sequence analyses of two isolates obtained from two different clinical materials clearly delineated a new species of the genus tsukamurella. this new species can be identified by its 16s ribosomal dna similarity values, as well as its physiological characteristics. the name tsukamurella inchonensis sp. nov. is proposed for these isolates, which are represented by strain immib d-771t (= dsm 44067t) (t = type strain). this strain exhibits only 45% dna r ... | 1995 | 8590680 |
| characterization of clinical isolates of pathogenic nocardia strains and related actinomycetes in thailand from 1996 to 2003. | in thailand from 1996 to 2003, 171 strains of pathogenic aerobic actinomycetes from clinical specimens were isolated. of those strains, 134 were mycolic acid containing actinomycetes, including 96 strains of nocardia species. others included 10 strains of gordonia, 14 strains of rhodococcus, and 22 strains of mycobacterium. one strain each of the genera tsukamurella and corynebacterium were also isolated. also identified were 27 strains of non-mycolic acid containing actinomycetes. our identific ... | 2005 | 15883719 |
| production of anti-gordonia amarae mycolic acid polyclonal antibody for detection of mycolic acid-containing bacteria in activated sludge foam. | mycolic acid-containing actinomycetes (mycolata) are considered the causative agents of foaming of activated sludge and scum formation in activated sludge treatment plants. in this study, the production of anti-gordonia amarae mycolic acid polyclonal antibodies was investigated. rabbits were immunized with a conjugate of keyhole limpet hemocyanin and mycolic acids of g. amarae, which contained 48 to 56 carbon atoms (average, 52.0). enzyme-linked immunosorbent assay (elisa) demonstrated that the ... | 2001 | 16233121 |
| [evaluation of api coryne system, version 2.0, for diphteroid gram-positive rods identification with clinical relevance]. | the ability of the api coryne system, version 2.0, to identify 178 strains of gram-positive rods was evaluated. seventy eight isolates belonged to genus corynebacterium and one hundred to related genera, all strains were isolated from clinical samples at the laboratory of bacteriology, hospital de clínicas josé de san martin (uba) between 1995 and 2004. the isolates were identified according to von graevenitz and funke's scheme. one hundred and sixty two out of 178 strains (91%) were correctly i ... | 2006 | 17370571 |
| culture and spray-drying of tsukamurella paurometabola c-924: stability of formulated powders. | the nematocidal agent, tsukamurella paurometabola c-924, was cultured in a 300 l bioreactor. spray-dried formulations of this microorganism were prepared using sucrose. at an outlet temperature 62 degrees c, survival rates between 12 and 85% were reached with sucrose up to 10% (w/w). the stability study of the powders showed that the best storage condition was at 4 degrees c under vacuum. a new method for the calculation of cell death order for bacteria stored at low temperatures was developed. ... | 2007 | 17603774 |
| tsukamurella spongiae sp. nov., a novel actinomycete isolated from a deep-water marine sponge. | a gram-positive, rod-shaped, non-spore-forming bacterium (strain k362(t)) was isolated from a deep-water marine sponge collected off the coast of curaçao in the netherlands antilles. on the basis of 16s rrna gene sequence similarities, strain k362(t) was shown to belong to the genus tsukamurella, being most closely related to tsukamurella pulmonis (99.2 %), tsukamurella tyrosinosolvens (98.9 %), tsukamurella strandjordii (98.8 %), tsukamurella pseudospumae (98.8 %) and tsukamurella spumae (98.8 ... | 2007 | 17625179 |
| tsukamurella: a cause of catheter-related bloodstream infections. | tsukamurellae are strictly aerobic gram-positive rods that can be easily misidentified as corynebacterium species, rhodococcus species, nocardia species, mycobacterium species, or other gram-positive aerobic rods. they have been uncommonly reported as a cause of different human infections, including bloodstream infections. we describe 2 new cases of catheter-related bloodstream infections (cr-bsi) caused by tsukamurella species and review 12 similar cases reported in the literature. conventional ... | 2009 | 18810513 |
| tsukamurella carboxydivorans sp. nov., a carbon monoxide-oxidizing actinomycete. | a gram-positive, slightly acid-alcohol-fast, carbon monoxide-oxidizing bacterium, strain y2(t), was isolated from a soil sample collected from a roadside in seoul, korea. on the basis of 16s rrna gene sequence comparative analyses, strain y2(t) was shown to belong to the genus tsukamurella and was most closely related to tsukamurella tyrosinosolvens dsm 44234(t) (genbank accession no. ay238514; 99.8 %). the predominant fatty acids were c(18 : 1)omega9c and c(16 : 0). the cell-wall peptidoglycan ... | 2009 | 19502350 |
| hoyosella altamirensis gen. nov., sp. nov., a new member of the order actinomycetales isolated from a cave biofilm. | a novel actinomycete, strain ofn s31(t), was isolated from a complex biofilm in the altamira cave, spain. a polyphasic study was carried out to clarify the taxonomic position of this strain. phylogenetic analysis with 16s rrna gene sequences of representatives of the genera corynebacterium, dietzia, gordonia, millisia, mycobacterium, nocardia, rhodococcus, segniliparus, skermania, tsukamurella and williamsia indicated that strain ofn s31(t) formed a distinct taxon in the 16s rrna gene tree that ... | 2009 | 19643882 |
| isolation and identification of dibutyl phthalate-degrading bacteria from hydrospheres in tokyo. | dibutyl phthalate (dbp) is used widely as a plasticizer and is thought to negatively affect various organisms. to isolate and investigate dbp-degrading bacteria from hydrospheres in tokyo, strains were selected on ynb medium containing dbp as the sole carbon source, and candidate strains were identified by zones of clearing around the colonies. degradation of dbp by the strains was subsequently measured with hplc, and bacterial identification was accomplished using 16s rdna sequences. nineteen s ... | 2009 | 19700919 |
| tsukamurella soli sp. nov., isolated from soil. | a gram-positive, rod-shaped, catalase-positive, oxidase-negative, white-coloured bacterium, designated strain js18-1(t), was isolated from a soil sample collected from halla mountain, jeju island, korea. 16s rrna gene sequence analysis revealed that the strain was most closely related to members of the genus tsukamurella with levels of sequence similarity of 95.4-96.5 %. strain js18-1(t) shared highest 16s rrna gene sequence similarity with tsukamurella strandjordii dsm 44573(t) (96.5 %), tsukam ... | 2010 | 19734289 |
| lipocarbazoles, secondary metabolites from tsukamurella pseudospumae acta 1857 with antioxidative activity. | a family of new secondary metabolites with a carbazole moiety and an alkyl side chain was isolated from tsukamurella pseudospumae strain acta 1857. they were named lipocarbazoles in accordance with their chemical structures, which were determined by mass spectrometry and nmr spectroscopy. lipocarbazoles are free radical scavengers showing antioxidative activity. | 2009 | 19848432 |
| immunochemical studies of trehalose-containing major glycolipid from tsukamurella pulmonis. | the chemical structure of the major glycolipid present in the chloroform-methanol extract of bacterial biomass of tsukamurella pulmonis is reported. this compound was purified by tlc and hplc. the sugar analysis revealed only glucose. detailed chemical analyses, nmr, and maldi ft-icr-mass spectrometric studies identified 2,3-di-o-acyl-alpha-d-glucopyranosyl-(1-->1)-alpha-d-glucopyranose as the final structure. short branched fatty acids (4:0 or 5:0) were linked to c-3 and saturated, mono, and di ... | 2010 | 20510396 |
| tsukamurella: an emerging opportunistic ocular pathogen. | | 2010 | 20628423 |
| genome sequence and characterization of the tsukamurella bacteriophage tpa2. | the formation of stable foam in activated sludge plants is a global problem for which control is difficult. these foams are often stabilized by hydrophobic mycolic acid-synthesizing actinobacteria, among which are tsukamurella spp. this paper describes the isolation from activated sludge of the novel double-stranded dna phage tpa2. this polyvalent siphoviridae family phage is lytic for most tsukamurella species. whole-genome sequencing reveals that the tpa2 genome is circularly permuted (61,440 ... | 2010 | 21183635 |
| tsukamurella infection: a rare cause of community-acquired pneumonia. | a 79-year-old asian man was admitted with community-acquired pneumonia. antimycobacterial therapy was initiated when sputum smears revealed acid fast bacilli. the patient was, however, diagnosed to have pneumonia secondary to tsukamurella spp. this is an exceedingly rare cause of pneumonia, especially in immunocompetent individuals. clinical presentation, diagnosis and treatment strategies of tsukamurella pneumonia are discussed with a literature review. | 2011 | 21613935 |
| successful immunotherapy of canine flea allergy with injected actinomycetales preparations. | aims: can heat-killed, borate-buffered suspensions of gordonia bronchialis, rhodococcus coprophilus or tsukamurella inchonensis be used to treat canine flea allergy? materials & methods: organisms cultured on sauton's medium into stationary phase were autoclaved in borate-buffered saline and stored at 10 mg wet weight/ml. intradermal injections of 0.1 ml containing 1 mg of bacilli were administered on the first and 20th days of the study. g. bronchialis and r. coprophilus were most effective in ... | 2011 | 21843084 |
| clinical characteristics of infections caused by tsukamurella spp. and antimicrobial susceptibilities of the isolates. | to investigate the clinical and microbiological characteristics of infections caused by tsukamurella spp., the computerised database of the bacteriology laboratory at national taiwan university hospital (taipei, taiwan) was reviewed retrospectively to identify patients with infections caused by this species during the period january 1997 to december 2008. all of the isolates had been initially misidentified as rhodococcus spp. identification of tsukamurella isolates to species level was carried ... | 2011 | 22014886 |
| complete genome sequence of tsukamurella paurometabola type strain (no. 33). | tsukamurella paurometabola corrig. (steinhaus 1941) collins et al. 1988 is the type species of the genus tsukamurella, which is the type genus to the family tsukamurellaceae. the species is not only of interest because of its isolated phylogenetic location, but also because it is a human opportunistic pathogen with some strains of the species reported to cause lung infection, lethal meningitis, and necrotizing tenosynovitis. this is the first completed genome sequence of a member of the genus ts ... | 2011 | 21886861 |
| tuberculosis-like pneumonias by the aerobic actinomycetes rhodococcus, tsukamurella and gordonia. | the order actinomycetales includes phylogenetically diverse but morphologically similar aerobic and anaerobic organisms, exhibiting filamentous branching structures which fragment into rods or coccoid forms. lung pathogens of the order comprise mycobacterium, nocardia, corynebacterium, actinomyces, kytococcus, rothia, williamsia, as well as gordonia, tsukamurella and rhodococcus. particularly, members of the last three genera are uncommon aerobic agents of lung cavitations and tuberculosis(tb)-l ... | 2011 | 22192786 |
| first case report of community-acquired pneumonia due to tsukamurella pulmonis. | | 2009 | 19153422 |
| brain abscess caused by tsukamurella tyrosinosolvens in an immunocompetent patient. | we describe a previously healthy patient with chronic otitis media complicated with cerebellar abscess caused by tsukamurella tyrosinosolvens. the organism was identified based on conventional biochemical identification methods, pcr-restriction fragment length polymorphism analysis of the hsp65 gene, and 16s rrna gene sequencing. the patient was treated successfully with debridements and prolonged antibiotic therapy. | 2009 | 19297591 |
| first report of tsukamurella keratitis: association between t. tyrosinosolvens and t. pulmonis and ophthalmologic infections. | we describe the first two cases of tsukamurella keratitis, presented as eye pain with or without blurring of vision. one case was associated with trichiasis and the other with contact lens wear. the two isolates were identified as t. tyrosinosolvens and t. pulmonis, respectively, by phenotypic characterization and 16s rrna sequencing. | 2009 | 19369436 |