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distribution of technetium-99m labeled corynebacterium parvum in normal and tumor bearing mice. 1977608499
distribution of 3h-thymidine-labelled c. parvum in mice.little is known of the localisation of injected corynebacterium parvum. we therefore developed a method of radiolabelling this vaccine and determined its distribution in the mouse. live c. parvum (wellcome, strain cn 6134) was grown in the presence of 3h-thymidine and subsequently killed by formalin. a high activity, 1-6 x 10(5) cpm/0.1 ml of a 7 mg dry weight/ml concentrated suspension, was obtained. its biological properties (hepatosplenomegaly and antitumour effects) were similar to those of ...1977608501
further studies on the enhancement of the antibody response in vitro by c. parvum.we have shown previously that spleen cells from mice pre-treated with c. parvum give enhanced antibody response when cultured in the presence of thymus-dependent antigens. in order to establish if c. parvum has a similar effect when added in vitro, spleen cells from untreated mice were cultured with c. parvum in marbrook chambers with sheep erythrocytes (srbc) as antigen. at concentrations of 35-140 microgram/ml/20 x 10(6) spleen cells c. parvum enhanced, but at higher concentrations suppressed ...1977608502
antibody responses, antitumour antibodies and ig class and subclass levels in c. parvum treated mice.this paper summarises the results of extensive studies performed in our laboratory during the past three years aimed at establishing the effect of c. parvum administration on humoral immunity in normal mice and mice previously challenged with viable mc fibrosarcoma cells. this treatment was found to (a) increase the response to thymus dependent and independent antigens, (b) result in the production of circulating immunoglobulins which bound to tumour cells and syngeneic tissues in vitro, and (c) ...1977608503
monocytosis-inducing activity (mia) of serum in corynebacterium parvum treated mice.intravenous injection of 548 microgram of c. parvum (lot 0407, mérieux laboratories, france) into c57bl mice produced rapidly appearing monocytopenia which was followed by marked and prolonged monocytosis after the third day. the serum of these animals, collected during the monocytopenic but not the monocytosis phase, showed monocytosis inducing activity (mia) as was demonstrated by the intravenous injection of the serum into normal test mice. serum from normal untreated mice or from mice given ...1977608504
cell mediated immunity to corynebacterium parvum and its role in tumor control.the injection of c. parvum provokes a transient cell-mediated immune response to its own bacterial antigens. during this period a subcutaneous challenge dose results in the appearance of a characteristic delayed type hypersensitivity (dth) skin reaction. if a suspension of syngeneic tumor cells is included in this challenge, they are destroyed in immune but not control animals. similarly, solid tumors of limited size can be made to regress under these conditions. adoptive transfer studies reveal ...1977608505
potentiation of anamnestic humoral responses by a single intravenous injection of corynebacterium parvum (c. parvum) in adult rabbits. 1977608507
effect of corynebacterium parvum on the immune response of the guinea pig to ovalbumin. 1977608508
in vitro anti-tumour properties of peritoneal exudate cells of conventional germ-free and stimulated mice.adherent cells of peritoneal exudates were obtained from conventional, or from germ-free mice or from mice having received in intraperitoneal infection of a variety of phlogogenic substances such as corynebacterium parvum (c. parvum) (mérieux) 500 microgram, thioglycolate (difco) 3 ml, bayol (esso) 0.50 ml + 0.50 ml culture medium, glycogene 1.2 ml. the cytotoxic properties adherent cells were studied in vitro by the chromium release technique (crt) and their cytostatic properties by the inhibit ...1977608509
effects and mode of action of corynebacterium parvum on murine tumour metastases.the effects of corynebacterium parvum (c. parvum) on lewis lung tumour metastases have been studied. i.v. and i.p., but not s.c. c. parvum significantly reduced pulmonary metastases. when combined with excision of the primary tumour, c. parvum given not more than 2 days before caused slight reduction of metastases, but when given earlier, it caused significant reduction and some mice were cured. metastases were increased by silica or cortisone acetate but were unaffected by trypan blue. the anti ...1977608510
effects of surgery and c. parvum on tumour metastases in mice.surgical operations depress immune responses. the aim of this study was to observe the effects of operation (amputation) on the growth of the lewis lung tumour and its metastases and to see if c. parvum counteracted them. anaesthesia alone, or with amputation, did not affect the growth of the primary tumour but c. parvum depressed it. anaesthesia did not affect the number of pulmonary metastases but amputation caused significant increase. c. parvum inhibited metastases and completely counteracte ...1977608511
a comparison of the antitumour activities of glucans and c. parvum.after i.v. injection of glucans (yeast cell walls, lentinan and pseudonigeran) into cba mice, the peritoneal macrophages became cytostatic to r1 leukemia cells in vitro, and the mice showed resistance to lung nodule formation by i.v. injected t3 fibrosarcoma cells. the glucans were less active than c. parvum. none of the glucans reproducibly acted as an adjuvant when mixed with irradiated tumour cells; nor did they inhibit the growth of a subcutaneous tumour when injected i.v. or directly into t ...1977608513
in vivo transfer of antitumor activity by peritoneal exudate cells from mice treated with c. parvum.we have investigated whether peritoneal exudate cells (pec) from c. parvum (cp) treated (c3hf/bu mice could transfer in vivo the resistance against a syngeneic fibrosarcoma (fsa). inhibition of tumour development and prolongation of survival of recipients were observed when cp-activated pec were admixed with fsa cells before their intraperitoneal (ip) or subcutaneous (sc) injections into normal mice. the antitumour activity increased with the increase of the ratio of effector to target cells. he ...1977608514
effects of c. parvum on radiation response of murine tumors.the effects of intravenous c. parvum (cp) on the efficacy of fractionated gamma-irradiation in treating a fairly immunogenic fibrosarcoma (fsa) and a weakly immunogenic mammary carcinoma (mdah-mca-4) were studied in c3hf/bu mice. tumors were 8 mm in diameter at the start of irradiation. the fsa was exposed to 500 and the mdah-mca-4 to 750 rads daily for 3.6 or 10 days. the administration of 0.25 mg cp varied from 4 days before to 14 days after the start of irradiation. cp greatly augmented radio ...1977608515
chemo-immunotherapy of mouse tumours using cyclophosphamide and c. parvum.treatment of a chemically induced mouse solid fibrosarcoma using either non-specific (c. parvum 350 mu i.v.), or specific active (s.c. c. parvum mixed with 5.10(5) irradiated tumour cells) immunotherapy, 4 days after a single dose of cyclophosphamide (200 mg/kg) was synergistically more effective than either c. parvum or drug treatment alone. a contributory factor may be that cyclophosphamide pretreatment has been shown to potentiate the specific antitumour immunity that arises from c. parvum in ...1977608516
increased sensitivity of c. parvum treated mice to ionizing whole body irradiation.we have investigated whether c. parvum (cp)-induced stimulation of hematopoiesis affects the survival of mice exposed to the whole body irradiation (wbi). c3hf/bu mice treated with cp exhibit an increased hematopoietic colony forming activity in their spleens and blood, but not in their bone marrow, as determined by the exogenous spleen colony assay. also, cp-treated c3hf/bu as well as cba mice show an increase in their endogenous colonies. this increased hematopoietic activity caused by cp trea ...1977608517
experimental evaluation of nonspecific immunostimulation and chemotherapy in the intracranial tumor-bearing fisher rat.one hundred sixty rats bearing intracranial tumors were divided into groups of 10 to evaluate c-i interval +, effect of chemotherapy alone (bcnu), chemotherapy and c. parvum, and c. parvum alone in the rat tumor model. all therapy was administered intraperitoneally: bcnu -5.2 mg/kg (40% of ld10) on the 9th and 16th post tumor implant day, and c. parvum-1.4 mg regardless of the body weight and the 6th or 23rd day and 6th and 23rd post tumor implant day. an additional group received bcnu on the 9t ...1977608518
the effect of pretreatment with corynebacterium parvum on the growth of a chemical induced transplanted murine tumor.unspecific immunostimulation with or without specific tumor immunotherapy may well contribute to the control of minimal residual cancer. corynebacterium parvum (c.p.) has been shown to raise the level of immunocompetence in tumor bearing animals. experiments are reported, which aim at an evaluation of optimal conditions for an immunostimulation with c.p. prior to the transplantation of balb/c meth a ascitis. using different dosages, routes of injection and schedules of vaccination it was found, ...1977608519
effects of bcg and c. parvum treatment on human melanoma xenografts.cba mice rendered immunodeficient by thymectomy, potentially lethal gamma-irradiation and reconstitution with bone marrow cells were used to grow a wide variety of human tumours as subcutaneous implants. samples of human melanoma obtained at surgery were successfully passaged by transplantation and produced rapidly growing tumours, some of which metastasized to lung, lymph nodes and the para-aortic node; this system was used as a model for the study of immunotherapy of melanoma. preliminary resu ...1977608525
the local effect of intravesical corynebacterium parvum in the dog. a preliminary study.high doses of c. parvum introduced in the bladder of the dog provoke changes in the submucosa. the significance and importance of these modifications should be investigated.1977608526
comparison of bcg and c. parvum preparations for adjuvant contact therapy of a rat sarcoma. 1977608529
histological changes of intravascular coagulation in mice resulting from intravenous injection of c. parvum.a histological study was made of liver and other tissues of mice at intervals after injection of c. parvum. as early as 20 min after injection occasional thrombi surrounded by polymorphs were seen in the alveolar capillaries. a general thrombosis rapidly developed in the hepatic vessels with consequent hepatic necrosis. thrombi also developed in splenic vessels. this intravascular coagulation was prolonged, lasting at least 7 days. there was evidence that fresh thrombi were being formed througho ...1977608532
effects of corynebacterium parvum vaccine on drug metabolism in the mouse.mice of the cba strain were given a single intraperitoneal injection of c. parvum vaccine at 50 mg/m2. there followed a progressive increase in liver weight and in the duration of hexobarbitone sleeping time, accompanied by a marked decrease in liver microsomal protein and in cytochrome p-450/mg microsomal protein. that is to say, content of cytochrome p-450 was reduced out of proportion to the apparent loss of endoplasmic reticulum. these changes were paralleled by decreases in microsomal o-dem ...1977608533
effects of bcg and corynebacterium parvum on immune reactivity in melanoma patients.sixteen patients with disseminated melanoma were immunised with either bcg (8 cases) or c. parvum (8 cases) on three occasions at 21 day intervals. blood for assay was taken immediately before the first immunisation and weekly for eight weeks thereafter. total white count tended to increase but little change was seen in lymphocyte and monocyte counts. serum igg increased after bcg but not with c. parvum, serum iga and igm did not alter. the 'e' rosette % did show some increase mainly after c. pa ...1977608536
the effect of corynebacterium parvum on human effector cells in peripheral blood. 1977608538
in vitro and in vivo effects of corynebacterium parvum on lymphocyte transformation.using a range of doses the effect of c. parvum on the in vitro response of lymphocytes to pha was studied. c. parvum was not found to be mitogenic itself when added to unfractioned lymphocyte cultures from normal donors and patients with breast cancer, nor did it effect the response to pha in these cultures except at the highest dose (20 microgram/100,000 cells) when it was inhibitory. 21 patients with breast cancer treated with c. parvum were assessed by their in vitro response to pha prior to, ...1977608539
the effect of intravenous infusion of corynebacterium parvum on an immune profile of women with breast cancer.an immunological profile has been measured in 21 patients with advanced breast cancer who were treated with c. parvum (wellcome strain cn6134). thirteen patients received a single i.v. dose of 15 mg of c. parvum and 8 received 4 mg i.v. on 5 successive days. the "profile" was recorded before and 7-10 days after treatment and included measurement of total white count, absolute lymphocyte and monocyte counts, pha response, b and t cell percentages. dncb and mantoux skin tests, immunoglobulin class ...1977608540
further studies on the serological effects of c. parvum immunotherapy in cancer patients.previous serological studies undertaken in our laboratory have shown that the repeated administration of small doses of c. parvum to a small group of cancer patients resulted in a consistant increase in the levels of igg (especially the igg2b subclass), the development of antibodies to c. parvum and the transient appearance of 'rheumatoid factor like' substances in the serum (br. j. cancer, 32, 310, 1975). in the present paper we report the results of similar studies in small numbers of (a) inop ...1977608542
first impressions of i.v. c. parvum in patients with solid tumours.6 patients with solid tumours (4 malignant melanomas, one fibrosarcoma, one osteogenic sarcoma) received i.v. c. parvum alone or in combination therapy (radiotherapy, levamisole and/or vitamin a). the single doses of c. parvum ranged from 5.0-7.5 mg/m2; no. of doses ranged from 1-8; interval between doses ranged from 2-140 days. 2 patients with malignant melanoma had no measurable disease, one of them (stage i) still has no evidence of disease. the patient with fibrosarcoma appeared to have a mi ...1977608543
experience with the quality control testing of c. parvum suspension for clinical trial.the control of c. parvum suspension presents special problems. the intended application of this preparation is in a therapeutic area where clinicians are accustomed to using defined chemical entities the biological activity of which are usually predictable according to weight of active principle administered, the route and number of doses. but the essential character of c. parvum is that of a vaccine in which the character of the active principle or principles is not clearly identified and can v ...1977608544
toxic and immunologic side effects of daily c. parvum-infusion in treatment-resistant cancer patients.daily increasing intravenous doses of corynebacterium parvum (c.p.) up to 5 mg/m2 i.v. x 10-14 days were given to 6 patients with widespread metastatic neoplastic diseases resistant to radio- and chemotherapy. the immunotherapy treatment-cycles were evaluated for toxic and immunologic side effects and also for possible clinical benefit to the patients. immunotherapy with i.v.-c.p. was moderately well tolerated. subjective discomfort for the patients (headache, chills, nausea) was not better tole ...1977608546
a survey of patients' reactions to intravenous corynebacterium parvum therapy.a survey was made of patients' reactions to high dose (21 mg) corynebacterium parvum therapy given intravenously over four hours. the patients completed a pro forma. rigors were the commonest side effect complained of being present in nearly all patients (24/25) and being very severe in one-third (10/25). other problems were nausea (15/25), vomiting (17/25), bone pain (12/25) and headaches (12/25). one-third also usffered with other less common side effects. overall, patients felt the side effec ...1977608547
intra-tumour c. parvum therapy in gastric carcinoma; a pilot study.in a preliminary and toxicological study, eight patients with carcinoma of the stomach were treated by corynebacterium parvum immunotherapy prior to surgical excision. killed c. parvum suspension was injected at multiple sites directly into the tumour mass with the aid of fibre-optic gastroscope at doses of 1.5 mg, 4 mg or 8 mg. each patient received two injections of the same dose with an interval of 14 days. the second injections were made 14 to 18 days prior to surgery. no chemotherapy was ad ...1977608548
mouse tumour tests for quality control of c. parvum preparations.description of two mouse tumor tests selected to control the quality of c. parvum pilot productions: transplanted ehrlich's ascitic tumor in inbred swiss mice and transplanted yc8 ascitic tumor in isogenic balb/c mice. the results obtained during three years of control are analyzed, particularly accuracy, long range adequacy and reproducibility of tumor challenges and c, parvum stimulations. the effects of some parameters of the tumor tests, e.g. dose-responses, treatment schedules, tumor-host r ...1977608550
c. parvum skin testing antigen: study on guinea pig model.a skin-testing antigen produced from c. parvum has been developed for exploring cell-mediated immunity and specially c. parvum specific cell-mediated immunity by delayed cutaneous hypersensitivity (dch) reaction. the dch antigen and the techniques of intradermal injection and multiple puncture are described. dch reactions are carried out in c. parvum specifically sensitized guinea pigs: sensitization procedure and adjuvant (ifa and cfa) effects are reported. measures of dch reactions for differe ...1977608551
proposals for quality control methods of bacterial vaccines for immunostimulation. i. general considerations.control methods for this type of vaccines include tests on identity, absence of contaminating microorganisms and safety which are required for any vaccine. in addition, for the control of vaccines for immunostimulation in cancer therapy some special methods for safety and potency testing are required. some assays are proposed, such as stimulation of lymphocytes in vivo and in vitro, stimulation of macrophages (listeria clearance) in mice and the prophylactic and therapeutic tumor model in mice. ...1977608552
the effect of intradermal administration of corynebacterium parvum on the immune response to hepatitis bs antigen.the immunopotentiating effect of the intradermal administration of a course of four doses (0.25 ml) of a standard suspension of killed c. parvum (2 mg/ml) was studied in a group of 10 asymptomatic chronic hepatitis b surface antigen (hbsag) carriers, as well as in 11 persons with antibodies to hbsag (anti-hbs) and six without hbsag or anti-hbs. hbsag, anti-hbs, and leukocyte migration inhibition (lmi) studies were performed in pre- and post-inoculation blood samples. c. parvum produced a substan ...1977609027
measurement of phagocytic activity of reticuloendothelial system (res) by intralipid: effect of c parvum treatment. 1977617547
[study of the tolerance of a submucous injection of coryné parvum in the dog bladder]. 1977617754
organ distribution of technetium-99m-labeled corynebacterium parvum in normal and tumor-bearing mice. 1978618580
correlation of antitumor chemoimmunotherapy with serum inhibition of tumor cell destruction.the administration of cyclophosphamide and corynebacterium parvum in combination results in tumor growth inhibition greater than that resulting from the use of either agent alone. the precise mechanism(s) by which this chemoimmunotherapy combination results in a synergistic inhibiting effect is not known. the possibility was entertained that the tumor effect might be related to a greater decrease in serum-mediated interference with cellular cytotoxicity, i.e., "blocking" activity, by both agents ...1978618581
influence of immunotherapeutic agents on the progression of spontaneously arising, metastasizing rat mammary adenocarcinomas of varying immunogenicities. 1978618584
immune responses during administration of adriamycin and corynebacterium parvum. 1978618689
[immunological and immunotherapeutical aspects of cancer (author's transl)]. 1978619481
[finding of anaerobic bacteria in blood cultures].out of the blood cultures sent to the bacteriology laboratory of hospital del niño dif, during the period of one year, in 1.4% of samples, isolation of anaerobic bacteria was obtained. isolation was more frequent in infants under one year of age, but specially in newborns. the species most frequently found were propionibacterium acnes, bacteroides melaninogenicus and clostridium subterminale. the presence of this finding in blood cultures of infants is noted, but there is no purpose to establish ...1978619923
heterologous immunity between piroplasms and malaria parasites: the simultaneous elimination of plasmodium vinckei and babesia microti from the blood of doubly infected mice.mice which have recovered from infections with the avirulent piroplasm babesia microti are also resistant to challenge with the virulent malaria parasite plasmodium vinckei. in mice infected with p. vinckei before the peak of the b. microti infection the numbers of malaria parasites in the blood increase until that peak and are then eliminated at the same time as the piroplasms. in mice infected with p. vinckei at or after the peak there is no apparent multiplication and the malaria parasites be ...1978622306
regional variations of cutaneous propionibacteria.propionibacterium acnes, p. avidum, and p. granulosum were quantitatively measured in 50 young adults. the scalp, forehead, external auditory canal, alae nasi, anterior nares, groin, rectum, and antecubital and popliteal fossa were sampled. these represent various cutaneous microenvironments, differing in moisture, density of sweat, sebaceous glands, and extent of anaerobiosis. these studies show that the propionibacteria are ubiquitous on the skin, with p. acnes predominant in both prevalence a ...1978623473
treatment of malignant ascitic and pleural effusion with corynebacterium parvum.six patients with malignant effusions, five from adenocarcinomas and one from a melanoma, were treated by intrapleural or intraperitoneal corynebacterium parvum. in each case there was a definite reduction in the effusions with a significant decrease in the number of malignant cells; in most cases the effusions stopped completely. although none of the patients lived for more than a year after treatment, they were undoubtedly more comfortable, as they no longer required frequent paracentesis. in ...1978623981
use of bacteriophage typing to distinguish propionibacterium acne types i and ii.strains of serotypes i and ii of propionibacterium were compared for phage sensitivity. the two serotypes could be distinguished by using a typing set consisting of 16 bacteriophages at concentrations that demonstrated selective lysis of serotype i or ii bacterial strains. seven phage types were found; three were composed exclusively of serotype i, and four were exclusively composed of serotype ii organisms. generally, serotype i strains were more sensitive to phage lysis than were serotype ii s ...1978624772
antitumor activity of purified cell walls from corynebacterium parvum.cell walls (cw), containing peptidoglycan and carbohydrate, were prepared from corynebacterium parvum and tested for lymphoreticular stimulation and antitumor effects in cba-t6t6 mice. cw did not induce splenomegaly. peritoneal macrophages became cytostatic to rl leukemia cells in vitro after ip injection of cw or of peptidoglycan but not of carbohydrate; however, on a dry-weight basis the activity was low (less than 10%) compared with that of c. parvum. tumor outgrowth was significantly suppres ...1978625069
immunotherapy of an established rat mammary adenocarcinoma (13762a) with intratumor injection of corynebacterium parvum.we studied the effects of intratumor injection of corynebacterium parvum vaccine on the survival of 13762a tumor-bearing rats. vaccine injection of established (7-day-old) tumors produced dose-related prolongation of survival and cured some animals. although 30 to 40% of the vaccine-injected primary tumors regressed, recurrences and continued growth of metastases ultimately killed one-fourth of the regressors. rats given 1500 microgram of c. parvum intratumorally at 7 days, with or without later ...1978626972
enhancement by corynebacterium parvum of the normal and tumor tissue response to hyperthermia.the effect of corynebacterium parvum treatment on the response of tumor and normal tissue to hyperthermia (43.5 degrees) was studied. animals were c3hf/sed mice from our defined flora mouse colony. the time at hyperthermia that achieved control of one-half of methylcholanthrene-induced fibrosarcomas and the foot reaction were examined after treatment. c. parvum, if given 3 to 32 days before hyperthermia, enhanced the reaction to local hyperthermia of normal tissue. no enhancement was observed if ...1978626985
natural cell-mediated cytotoxicity in rats. ii. in vivo augmentation of nk-cell activity.natural cell-mediated cytotoxicity in rats as well as in mice has been shown to vary consistently with age, with peak levels detectable at 5-10 weeks. the levels of cell-mediated cytotoxicity against tumor cells could be augmented in strains of inbred rats with either high or low levels of natural reactivity, by ip injection of a variety of agents, including c. parvum, lcmv, krv, and poly i:c. the specificity of the augmented cytotoxicity appeared to be the same as the specificity of natural kil ...1978627428
corynebacterium parvum-induced resistance to a methylcholanthrene fibrosarcoma. 1978627989
modification of radiation responses of murine tumors by misonidazole (ro 07-0582), host immune capability, and corynebacterium parvum.the hypoxic cell sensitizer misonidazole (ro 07-0582),1-(2-nitro-1-imidazolyl)-3-methoxy-2-propanol, significantly enhanced the local control of the weakly immunogenic c3h mouse mammary carcinoma mdah-mca-4 (8-mm diameter) by single doses of radiation. the dose modification factor (dmf) was 2.33 when the drug was given ip to inbred c3hf/bu mice in a dose of 1 mg/g body weight 30 minutes before irradiation of the tumor. the dmf in a highly immunogenic 3-methylcholanthrene-induced c3h fibrosarcoma ...1978633396
immunotherapy of cancer with corynebacterium parvum. 1978637374
comparative studies of porphyrin production in propionibacterium acnes and propionibacterium granulosum.porphyrin production by propionibacterium acnes and that by propionibacterium granulosum were compared. porphyrin synthesized by both organisms was identified as coproporphyrin iii on the bases of absorption and fluorescence spectra and behavior on paper chromatography and thin-layer chromatography. quantitative, rather than qualitative, differences in production were found between these organisms. in general, p. granulosum produced significantly greater amounts (p less than 0.001) of porphyrin ...1978637914
studies with a spontaneous mouse tumor. i. growth in normal mice and response to corynebacterium parvum.growth of isogeneic transplants of a spontaneous murine adenocarcinoma, which is virtually devoid of tumour-specific transplantation antigens, is inhibited by i.v. injection of c. parvum 3 days after tumour inoculation, or by mixing a small dose of c. parvum with the tumour inoculum. moreover, the therapeutic effect of cyclophosphamide, followed by i.v. or i.p. injection of c. parvum 5 days later, on established transplants of the same tumour is greater than that of cyclophosphamide alone. these ...1978638014
endophthalmitis caused by propionibacterium acnes.we treated a man who developed endopthalmitis six weeks after a seemingly minor injury. cultures were positive for propionibacterium acnes, an anerobic, gram-positive rod that is a normal inhabitant of the conjunctiva but is rarely a cause of endophthalmitis. the patient underwent vitrectomy and lensectomy with intravitreal injection of antibiotics followed by systemic cephalothin and prednisone. clinical and visual improvement was rapid.1978638845
phase i studies of c. parvum given intravenously: effects of dexamethasone on its biologic and toxic properties.three biologic effects were clearly associated with c. parvum infusions: a) granulocytosis with lymphomonocytopenia, b) increase in responsiveness to phytohemmagglutinin and c) increase of specific antibodies to c. parvum antigens. addition of dexamethasone moderately altered the kinetics of leukocyte changes without affecting other properties. side effects after intravenous administration of c. parvum (2.5 mg/m2) consisted primarily of chills and fever which lasted for periods of 6-24 hours. s ...1978638994
thymoindependent antigentic stimulation in nude mice: response to polyvinylpyrrolidone and adjuvant effects of corynebacterium parvum and lh1.the humoral thymoindependent response to polyvinylpyrrolidone (pvp) measured by the farr test, was not different in athymic nude and phenotypically normal mice. in athymic and normal mice, this response was significantly increased by cornebacterium parvum administered at the time of immunization. a recently synthetized compound, lh1 (3alpha-n-[2 aminoethanol]-amino 5-alpha-pregnane-20-one) was, however, a better immunostimulant of the anti-pvp response in athymic mice.1978640744
effects of amputation and corynebacterium parvum on tumour metastases in mice.the effects of operation (lower-limb amputation) on the growth of the lewis lung tumour and its metastases were studied. the role of c. parvum in counteracting these effects was investigated. anaesthesia alone or with amputation did not affect primary tumour growth. c. parvum depressed this growth. anaesthesia did not affect the number of pulmonary metastases, but amputation caused a significant increase. c. parvum inhibited metastases and completely counteracted the effects of operation on them ...1978646927
corynebacterium parvum enhances colonic cancer in dimethylhydrazine-treated rats. 1978646934
decreased hepatic drug demethylation in patients receiving chemo-immunotherapy.the effect of immunotherapy and chemotherapy on hepatic n-demethylation of aminopyrine was studied by means of the aminopyrine breath test (abt) in 32 patients with cancer. the aminopyrine breath test (abt) was decreased in 3 of 11 patients (27.3%) receiving intradermal bcg (+/- dtic) at a dose of 3 x 10(7) viable organisms. one of 4 (25%) patients receiving intradermal bcg (+/- dtic) at 3 x 10(8) viable organisms per dose developed an altered abt. changes were not seen in patients receiving aer ...1978647621
monocyte function in patients with solid neoplasms during immunotherapy with corynebacterium parvum.the present studies were performed in order to evaluate monocyte function in patients with solid neoplasms before and after administration of c. parvum. the results demonstrate that monocytes from cancer patients display increased numbers of c3 and fc receptor sites after administration of c. parvum. it is concluded that characterization of monocyte receptor activity may be helpful in monitoring the effects of immunotherapy in the immune system.1978647625
induction of spleen cell growth and dna polymerase activity by corynebacterium parvum.corynebacterium parvum induces rapid proliferation of spleen cells with concomitant rapid increases in dna synthesis as measured by [3h]thymidine uptake and increase in dna polymerase activity. cell number increased exponentially over 10 days. dna polymerase activity increased 8-fold after c. parvum stimulation. the rapidity of response indicated a population response to the stimulus, and the data are consistent with a direct stimulation of spleen cell proliferation by c. parvum.1978647675
effect of aerobic and anaerobic corynebacteria on reaginic antibody response in mice. 1978661628
radiation-induced inhibition of splenocyte locomotion and its protection by c. parvum. 1978663069
prospective randomized evaluation of the role of limb-sparing surgery, radiation therapy, and adjuvant chemoimmunotherapy in the treatment of adult soft-tissue sarcomas. 1978663826
nephrotoxicity from cancer immunotherapy.because systemic intravenous immunotherapy with corynebacterium parvum is an effective immunopotentiating and immunotherapeutic agent in animals, clinical studies of this agent have been undertaken. toxicities in man have been noted, but most are treated symptomatically. three patients with metastatic melanoma developed oliguria, edema, diffuse bilateral pulmonary infiltrates, azotemia, hypoalbuminemia and hypocomplementemia, while receiving intravenous c. parvum therapy. all had renal biopsies ...1978666183
immunity to transplantable carcinogen-induced fibrosarcomas in b2/b2 chickens. iii. tumor growth inhibition by local delayed hypersensitivity reactions to unrelated antigens.delayed hypersensitivity (dh) reaction to human ig, corynebacterium parvum or allogeneic cells at the site of tumor cell injection, suppressed the fibrosarcoma (scfs) growth in chickens. spleen cells of sc chickens sensitized to human ig or c. parvum suppressed scfs growth when adoptively transferred with tumor cells, but only when the sensitizing antigen was present locally. this suppression did not occur in irradiated recipients, scfs i cells injected into wattles of chickens immune to the tum ...1978668800
response of malignant melanoma to plasma exchange, surgical debulking, and corynebacterium parvum.a 48 year old man with extensive cutaneous and subcutaneous metastatic malignant melanoma responded to treatment by repeated plasma exchanges, partial surgical debulking of tumor masses, and intravenous corynebacterium parvum. circulating immune complexes were measured by the serum c1q binding method. tumor regression coincided with serum c1q binding reduction to normal levels. clinical relapse was preceeded by recurrent elevation of serum c1q binding activity.1978669835
effects of corynebacterium parvum and cortisone on the primary lewis tumour and its metastases.the effects of corynebacterium parvum and cortisone acetate (ca) on the primary lewis lung carcinoma and its pulmonary metastases were investigated. c. parvum given iv either on the same day or 7 days after tumour inoculation, reduced primary tumour growth, while 2.5 mg ca (high-dose) given sc 4 and 11 days after tumour, alone or in combination with c. parvum, administered on day 0, reduced primary tumour growth to the same extent as c. pravum alone. high-dose ca given on days 2 and 6 had no eff ...1978669856
induction of interferon in mice injected with heat-killed corynebacterium anaerobium. 1978671815
electrophoretic protein patterns and enzyme mobilities in anaerobic coryneforms.the soluble protein patterns and electrophoretic mobilities of malate and succinate dehydrogenases and catalase have been examined in 25 strains of propionibacterium acnes, p. granulosum, and p. avidum. a distinctive protein pattern for each species was found, and it was possible also to distinguish the serotypes within p. acnes and p. avidum. strains of p. acnes, p. granulosum, and p. avidum could be differentiated by the mobilities of their malate dehydrogenases. catalase activity was detected ...1978677876
anaerobic flora of the normal human conjunctival sac.specimens from the conjunctival sacs of 92 healthy eyes were cultured on two separate occasions to determine the presence or absence of a persistent anaerobic flora. aerobic bacteria and fungi were also studied for comparison. of the 184 eye cultures, 112 (60.9%) contained at least one microorganism. obligate anaerobes were recovered from 51.6% (95/184) of the cultures. propionibacterium acnes, the predominant anaerobe encountered, was present in 49.5% (91/184) of the eyes. aerobic and facultati ...1978678185
coagulation and fibrinolysis during the infusion of corynebacterium parvum in man. 1978678428
effect of corynebacterium parvum on the proliferative rate of granulocyte-macrophage progenitor cells and the toxicity of chemotherapy.we have studied the interaction of corynebacterium parvum and a variety of cell cycle-specific and cell cycle-nonspecific chemotherapeutic agents on granulocyte-macrophage progenitor cells [colony-forming units culture (cfu-c)] in c57bl/6 mice. in vitro tritiated thymidine suicide studies showed an increased rate of proliferation of bone marrow cfu-c 24 hr after c. parvum injection. in vivo toxicity of cell cycle-specific agents but not of cell cycle-nonspecific agents for bone marrow cfu-c was ...1978679169
comparison of the inhibition of tumor growth following local or systemic administration of corynebacterium parvum or other immunostimulating agents with or without cyclophosphamide. 1978679170
rate of tumour inhibition by activated macrophages in vitro. 1978679868
cytotoxic activity of human blood monocytes against cultured breast cancer cells.cytotoxic activity of blood monocytes from 19 healthy individuals was examined in vitro against the cultured floating cells of human breast cancer origin. target tumor cells inoculated on monocyte cultures and maintained for 5 days were reduced in number, compared with the control cultues of tumor cells alone. increased cytotoxicity of the monocytes was noted after in vitro activation by heat-killed corynebacterium liquefaciens (propionibacterium acnes). c. liquefaciens of the same dose as used ...1978680464
interferon production and lymphocyte stimulation in human leucocyte cultures stimulated by corynebacterium parvum.killed c. parvum organisms stimulated a lymphoproliferative response in human peripheral leucocyte cultures of both adult and cord blood origin. they also induced high titres of interferon in cultures of adult leucocytes, but there was no correlation between the degree of lymphocyte stimulation and of interferon production. a considerable variability between donors was seen in both assays. the amount of interferon produced in c. parvum-stimulated cultures was considerably higher than that stimul ...1978688696
in vitro activity of tiamulin (81.723 hfu), a new pleuromulin derivative, against clinically significant anaerobes.the susceptibility of more than 40 strains of gram-negative and gram-positive anaerobes to tiamulin (sandoz 81.723 hfu), a new pleuromulin (pleuromutilin) derivative, was determined by broth dilution and agar dilution tests. the influences of density of the inoculum upon mics was studied by a specially designed pour plate-technique. bacteroides fragilis, b. vulgatus, b. splanchnicus, b. oralis, b. asaccharolyticus, b. melaninogenicus, fusobacterium fusiforme (f. nucleatum), sphaerophorus necroph ...1978690009
preliminary observations on the use of corynebacterium parvum in patients with primary intracranial tumors: effect on intracranial pressure.it has been observed that patients with metastatic brain lesions developed neurologic symptoms when given corynebacterium parvum. to obtain more information in patients with intracranial mass lesions, intracranial pressures (icp) were measured or observed in six patients undergoing c. parvum therapy. all patients except two (one previously shunted, one recently operated) demonstrated a profound increase in icp. it is presumed that either edema is formed within brain and tumor tissue (inasmuch as ...1978692136
immunoproliferative alterations in lymphoid tissues and liver in mice stimulated with corynebacterium parvum.morphological alterations in the spleen, liver, lymph nodes, thymus, bone marrow and blood were studied in dba and balb/c mice treated with corynebacterium parvum. the spleen lymph nodes and liver increased in size and weight, whereas thymus showed decrease in weight compared to the control group. a marked, early proliferation of monocytes in the bone marrow preceded infiltrations by macrophages in lymph nodes, spleen and liver. these infiltrations were closely related to the vascular structures ...1978696333
failure of preoperative c. parvum vaccine to modify secondary disease following excision of two non-immunogenic murine carcinomas.sadler and castro (1976) reported that a single dose of c. parvum vaccine given i.p. or i.v. to mice 4 days before excision of subcutaneous transplants of lewis lung carcinoma significantly reduced the incidence of lung metastases in the operated mice. in similarly designed experiments, using 2 different carcinomas of spontaneous origin in our own inbred mouse colonies, we were unable to demonstrate any influence of c. parvum vaccine on the incidence or latent period of secondary disease in oper ...1978698036
bacteriologic examination of autologous blood. 1978705401
a preliminary study of intralesional, intralymph node, intravenous and intraperitoneal corynebacterium parvum treatments in patients with advanced cancer.twenty-one patients with squamous cell carcinoma of oral cavity and/or cervical lymph node and four patients with soft tissue sarcomas were given intralesional (2-4 mg/m2) and intralymph node (2-4 mg/m2) c. parvum injections followed by 5 intravenous (2-4 mg/m2) c. parvum treatments and conventional therapies. seven patients with stage iii ovarian carcinomas were given intraperitoneal (2-4 mg/m2) c. parvum injections followed by conventional therapies. the local and systemic effects of the c. pa ...1978709538
combined radiotherapy and corynebacterium parvum treatment of rat tumors with different immunogenicity. 1978711536
lysozyme levels and macrophage content of tumor tissue in c3h mice bearing fibrosarcoma transplants treated by radiation and corynebacterium parvum. 1978711553
bacteriological investigation on biopsy specimens from patients with sarcoidosis.biopsy specimens, mostly lymph nodes, removed under aseptic conditions, were emulsified and the homogenate was inoculated in various media, which were incubated aerobically or anaerobically. only propionibacterium acnes was isolated in high incidence and the highest concentration of bacteria was 6.5 x 10(5) per g tissue: 28 and 31 out of 40 lymph nodes were positive on solid and on liquid media, respectively. p.acnes was also isolated from control non-sarcoidosis lymph nodes: 4 and 8 out of 14 w ...1978713130
characterization of propionibacterium acnes isolated from biopsy specimens obtained from patients with sarcoidosis. 1978713132
pigment vs cholesterol cholelithiasis: bacteriology of gallbladder stone, bile, and tissue correlated with biliary lipid analysis. 1978717347
toxicity manifestations following intravenous corynebacterium parvum administration to patients with ovarian and cervical carcinoma.manifestations of clinical toxicity were evaluated following 341 courses of intravenous corynebacterium parvum adjuvant immunotherapy in patients with ovarian and cervical carcinoma. most patients exhibited symptoms of minor toxicity, which decreased in intensity as subsequent courses of therapy were administered. temperature elevations to 38.5 degrees c. were the most objectively measured signs of toxicity but temperature elevations greater than 38.5 degrees c. occurred following only 20.5 per ...1978717456
destruction of regional lymph node metastases of rat mammary adenocarcinoma 13762a by treatment with corynebacterium parvum.intratumoral administration of corynebacterium parvum to 13762a tumor-bearing rats on day 7 of tumor growth, followed by primary tumor excision on day 20, regularly cured about 40% of the animals and significantly prolonged survival in the remainder. rats treated by surgery alone on either day 7 or day 20 died with metastases to axillary lymph nodes and lungs. tumor was established in axillary lymph nodes by day 7. therefore, intratumoral injection of c. parvum on day 7 destroyed metastases alre ...1978719637
positive 67ga-citrate scans in patients receiving corynebacterium parvum.gallium-67 citrate imaging for the staging of lymphomas was performed in three patients receiving corynebacterium parvum (c. parvum) immunotherapy. unexpected uptake at the site of c. parvum injections was identified and although the mechanism of localization is not determined by this study, similarities between c. parvum's known actions and the postulated mechanism of 67ga-citrate localization are compared.1978719980
immune prophylaxis of experimental cancer by corynebacterium parvum.fractionated administration of c.p. i.v. in mice at risk from sarcomas after injection of 0.2 mg mca leads to a significant prolongation of the period of latency under certain circumstances. experimental groups which have received the immune stimulator for 6 weeks immediately after the cancerogenic factor show the best results. after tumour manifestation there is no perceptible preventive effect of c.p.1978720440
further observations on the inhibition of tumor growth by corynebacterium parvum with cyclophosphamide--viii. 1978721660
electron capture gas-liquid chromatographic study of metabolites produced by some arthritic transudate-associated organisms in vitro and in vivo in rabbit models.computerized, frequency-pulsed, modulated electron capture gas-liquid chromatography was used to study the acid metabolites produced in vitro in fetal calf serum and in vivo in an animal chamber model. several strains of diplostreptococcus agalactiae, propionibacterium acnes, staphylococcus aureus, and streptococcus serogroups a, b, and g were studied. all of these organisms have been reported to be associated with arthritic transudates in humans. metabolites were detected by this method from de ...1978721944
differential susceptibility of propionibacterium acnes, p. granulosum and p. avidum to free fatty acids.the susceptibility of 98 propionibacterium acnes, p. granulosum and p. avidum strains isolated from acne cases and appropriate reference strains to decanoic, dodecanoic, octadeca-9,12 dienoic, and octadeca-9,12,15 trienoic acids was investigated using minimal inhibitory concentration estimation by an agar dilution techique. the tested acids showed their relative antibacterial activity in the following order: c18:3 delta 9,12,15 greater than c18:2 delta 9,12 greater than c12:0 greater than c10:0 ...1978722112
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