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the sulfated polysaccharide-peptidoglycan complex from an arthrobacter species: characterization of the linkage between the two components.further structural features of the sulfated polysaccharide-peptidoglycan complex, which is produced by an arthrobacter sp. and contains phosphorus as its minor component, were investigated. phosphoric acid esters such as d-glucose 6-phosphate, glycerol 1-phosphate and muramic acid phosphate were isolated from the acid hydrolysate of the complex. on mild acid treatment, the complex became positive for both the morgan-elson reaction and acid phosphatase digestion. the mild acid hydrolysate readily ...19836137481
preparation of gdp-d-mannose specifically labeled with tritium in the d-mannosyl moiety. 19806158275
the synthesis of cytokinin-like substances by coryneform bacteria isolated from the roots of pine seedlings (pinus silvestris l.).seven of ten strains studied produced cytokinin-like substances, mainly in 7 day old cultures. the cytokinin-like substances were most often located on chromatograms at rf values 0.8 to 0.9, rarely at rf 0.6 to 0.7.19806158833
synthesis of macromolecules in synchronously grown arthrobacter.the synchronous growth of arthrobacter has not yet been studied. we have shown that synchrony of a. crystallopoietes and a. globiformis cultures can be achieved in a liquid synthetic medium by inoculating a fresh medium with cells taken from an advanced stationary phase culture. dna content measurements and pulsed incorporations of tritiated thymidine have been used to show that dna synthesis occurs in a stepwise manner in these synchronized cultures. the use of absorbance of cultures as a monit ...19806159519
effect of microorganisms on the phytotoxicity of herbicides. vi. modification of venzar activity by bacterial cultures.interactions between two bacterial strains and venzar were compared. it was found that the mechanism of interactions is various and causes the modification of herbicide phytotoxicity. metabolites of bacillus subtilis 72 interfered with herbicide by affecting physiological processes in plant tissues and enhancing its inhibitory influence. arthrobacter sp. 18 strain decreased the phytoinhibitory effect of herbicide due to conjugation with the carrier from venzar.19816168180
a phylogenetic analysis of staphylococci, peptococcus saccharolyticus and micrococcus mucilaginosus.the intra- and intergeneric relationships of the genus staphylococcus, and the phylogenetic position of peptococcus saccharolyticus and micrococcus (staphylococcus salivarius), were investigated by comparative oligonucleotide cataloguing of 16s rrna. all the staphylococci investigated form a phylogenetically coherent group at the genus level that, in addition, contains the anaerobic species peptococcus saccharolyticus. the genus staphylococcus belongs to the broad bacillus-lactobacillus-streptoc ...19816172548
nucleic acid hybridization studies on microbacterium, curtobacterium, agromyces and related taxa.thirty strains of agromyces, arthrobacter, curtobacterium, brevibacterium, corynebacterium and microbacterium, exhibiting the rare peptidoglycan of group b, were subjected to extensive nucleic acid hybridization studies. the dna homology values indicate that corynebacterium insidiosum dsm 20157 is genetically identical with corynebacterium michiganense dsm 20134. corynebacterium sepedonicum ncppb 378 and corynebacterium nebraskense dsm 20400 are closely related to corynebacterium michiganense ds ...19826183393
production of glutamic acid by an arthrobacter sp. i. identification and nutritional requirement in relation to glutamic acid production. 19826188337
microflora of antarctic krill (euphausia superba). 19826188340
a phylogenetic analysis of the family dermatophilaceae.the comparative analysis of the 16s ribosomal ribonucleic acid (rrna) of geodermatophilus obscurus dsm 43060 and dermatophilus congolensis dsm 43037 revealed that these members of the family dermatophilaceae were only remotely related. while g. obscurus represented an individual and separate line of descent within the phylogenetically defined order actinomycetales, d. congolensis was closely related to representatives of arthrobacter, micrococcus, cellulomonas, brevibacterium, promicromonospora ...19836195302
effect of ph on production of cytokinin-like substances by bacteria isolated from soil, rhizosphere and mycorrhizosphere of pine (pinus silvestris l.).cytokinin-like substances were produced in higher amounts by soil isolates than by those isolated from the rhizosphere and mycorrhizosphere. however more bacterial strains isolated from the rhizosphere and mycorrhizosphere were capable of synthesizing cytokinin-like compounds. a distinct effect of ph on the production of these substances was found. it is suggested that zeatin and zeatin riboside are likely to be among the cytokinin-like substances detected.19846205554
interaction between bacterial metabolites and some pesticides. ii. change of phytotoxicity of the herbicide roneet by the phenolic metabolites of arthrobacter sp.the bacteria from arthrobacter genus isolated from sugar beet rhizosphere were found to produce gallic, protocatechuic, p-hydroxybenzoic, syryngic, vanillic, veratric acids, p-quinone and two unidentified phenolic compounds. the mixture of the bacterial phenolic compounds increased the phytotoxicity of roneet, inhibiting the germination of wheat. model experiments showed that the phenolic acids used with the herbicide roneet increased its phytotoxicity and p-hydroxybenzoic acid was found to be t ...19846209927
generic composition and physiological and cultural properties of heterotrophic bacteria isolated from soil, rhizosphere and mycorrhizosphere of pine (pinus silvestris l.).among the bacteria studied arthrobacter globiformis was predominating in the root zone, while in the non-rhizosphere soil most numerous were bacillus circulans and a. globiformis. ammonifiers were more numerous among the root zone bacteria than among the root free soil organisms. the reverse was noted with bacteria capable of hydrolysing starch, cellulose, pectin and chitin.19846209931
siderophore production by vibrio vulnificus.previous studies in our laboratory, as well as clinical evidence, have suggested that increased iron levels in the host may be important in infections caused by the halophilic pathogen vibrio vulnificus. to study iron acquisition, we induced siderophore production by growth in a low-iron medium, and biochemical testing indicated the production of both hydroxamate- and phenolate-type siderophores. the siderophores were extracted from growth filtrates with ethyl acetate (for phenolates) and phenol ...19836223882
siderophore-mediated iron uptake in different strains of anabaena sp.anabaena sp. strain 6411, which produces the dihydroxamate siderophore schizokinen to facilitate iron uptake, is also capable of using the related siderophore aerobactin. the two siderophores compete for the same iron transport system, but there is a markedly higher affinity for ferric schizokinen than for ferric aerobactin. the trihydroxamate siderophore ferrioxamine b is far less effective as an iron donor in this organism. anabaena sp. strain 7120 appears to be closely related to strain 6411. ...19836227608
extracellular siderophores from aspergillus ochraceous.a large number of iron-chelating compounds (siderophores) were isolated from supernatants of iron-deficient cultures of a mold isolate, subsequently identified as aspergillus ochraceous . siderophores in their iron chelate form were purified to homogeneity by using bio-gel p2, silica gel, and c-18 bonded silica gel (reverse-phase) columns. most of these compounds, as identified by 1h and 13c nuclear magnetic resonance spectroscopy and x-ray crystallography, belong to the ferrichrome family. the ...19846233261
the synthesis of 4-methylumbelliferyl alpha-ketoside of n-acetylneuraminic acid and its use in a fluorometric assay for neuraminidase. 19806243885
[1h-nmr-spectroscopic evidence for the release of n-acetyl-alpha-d-neuraminic acid as the first product of neuraminidase action (author's transl)].the 1h-nmr spectroscopy was used to study the anomeric configuration of n-acetyl-d-neuraminic acid released by the action of neuraminidase. the hydrolysis of neuacalpha 2 leads to 3 gal-beta 1 leads to 4glc (20mm) by the enzymes of clostridium perfringens and arthrobacter ureafaciens (50 mu, 150 mu and 800 mu, respectively) in 50mm na/k-phosphate buffer pd 5.4 was observed by recording the spectra. on the basis of the characteristic signals of the protons at c-3 (alphaneuac: delta[h(3e)] = 2.72, ...19806253376
myceloid growth of arthrobacter globiformis and other arthrobacter species.transitory myceloid growth occurs in certain complex media with arthrobacter globiformis strain atcc 8010. this type of growth, however, was not observed in a medium which contained an array of metal ions but did not contain agents able to complex metal ions. addition of metal-complexing agents to this medium caused an interruption in the life cycle of strain 8010 so that growth occurred only as the myceloid form. it appeared that manganese was the critical metal that was removed by the metal-co ...19806254945
a comparison of the cytochrome complements of seven strains of marine manganese-oxidizing bacteria. 19806259842
[dna-methylase from arthrobacter luteus screens dna from the action of site-specific endonuclease alu i].dna-methylase was isolated from a cell extract of a. luteus and partially purified by chromatography on phosphocellulose. the purified enzyme methylates dna of phage lambda and plasmids pbr322, thus making them resistant to a subsequent action of endonuclease alu i. it has been shown that cytosine is the object of methylation within dna. this modification does not screen dna from the action of site-specific endonucleases sal i, bam hi, ecor i, ecor ii, xho i and xho ii. it has been experimentall ...19816268201
[1h-nmr spectroscopy--a potent method for the determination of substrate specificity of sialidases (author's transl)].we describe here the application of 1h-nmr spectroscopy to determine the substrate specificity of sialidases using a 1:1 mixture of neuac alpha 2-3gal beta 1-4glc and neuac alpha 2-6gal beta 1-4glc, one viral and five bacterial sialidases. this method utilizes the separate signals in nmr spectra, characteristic for the different alpha ketosidically linked neuac residues and also for bound and free neuac. the signals generally most suitable for these purposes are those of h3a, h3e and ncoch3. by ...19816273284
a sulfated polysaccharide produced by an arthrobacter species.a new sulfated polysaccharide was isolated from the culture supernatant of a strain of arthrobacter sp. the polysaccharide purified with quaternary ammonium salts consists of d-galactose, d-glucose, sulfate, phosphorus, glucosamine, muramic acid, alanine, glutamic acid, glycine, and ll-diaminopimelic acid in a molar ratio of 56 : 9.0 : 68 : 6.4 : 2.0 : 1.1 : 2.1 : 1.0 : 1.2 : 1.2. the presence of the two amino sugars and four amino acids suggests that the polysaccharide, which is principally a g ...19826298190
the specificity of viral and bacterial sialidases for alpha(2-3)- and alpha(2-6)-linked sialic acids in glycoproteins.the anomeric specificity of six sialidases (vibrio cholerae, arthrobacter ureafaciens, clostridium perfringens, newcastle disease virus, fowl plague virus and influenza a2 virus sialidases) was assessed with sialylated antifreeze glycoprotein, ovine submandibular gland glycoprotein and alpha 1-acid glycoprotein, resialylated specifically in alpha(2-3) or alpha(2-6) linkage with n-acetylneuraminic acid or n-glycolylneuraminic acid using highly purified sialyltransferases. the rate of release of s ...19836301560
the effect of gyrase inhibitors and cyclic amp on induction and glucose repression of the 6-hydroxy-nicotine oxidases in arthrobacter oxidans.the induction by d,l-nicotine of the enantiozymes 6-hydroxy-l-nicotine oxidase and 6-hydroxy-d-nicotine oxidase in arthrobacter oxidans was differently affected by the inhibitors of escherichia coli gyrase, novobiocin and nalidixic acid. these compounds inhibited 6-hydroxy-l-nicotine oxidase induction slightly, but led to an increase in the level of 6-hydroxy-d-nicotine oxidase activity. furthermore, the specific repression by glucose of 6-hydroxy-d-nicotine oxidase synthesis was not abolished b ...19826303240
novel oligosaccharide chains on polysialoglycoproteins isolated from rainbow trout eggs. a unique carbohydrate sequence with a sialidase-resistant sialyl group, dga1nac beta 1 leads to 4(neugc2 leads to 3)dga1nac.a series of novel carbohydrate chains all possessing a hitherto unknown trisaccharide unit, ga1nac beta 1 leads to 4-(neugc2 leads to 3)ga1nac beta 1 leads to, have been isolated from trout egg polysialoglycoproteins, a new class of glycoproteins, on alkali-borohydride treatment. on the basis of chemical (methylation, smith degradation, and hydrazinolysis-nitrous deamination) and direct-probe mass spectrometric methods. deamination) and direct-probe mass spectrometric methods, the structures of ...19846320864
fish egg polysialoglycoproteins: circular dichroism and proton nuclear magnetic resonance studies of novel oligosaccharide units containing one sialidase-resistant n-glycolylneuraminic acid residue in each molecule.long-core units having the common sequence ga1nac beta 1 leads to 4(neugc2 leads to 3)ga1nac beta 1 leads to 3gal beta 1 leads to 4ga1 beta 1 leads to 3ga1nac are one of the major constituents of rainbow trout egg polysialoglycoproteins. the existing ambiguity regarding the anomeric configuration of the sialidase-resistant unsubstituted sialyl group present in this novel type of oligosaccharide chains has been resolved by a circular dichroism difference spectral method. the fact that the negativ ...19846320865
isolation of mannan-protein complexes from viable cells of saccharomyces cerevisiae x2180-1a wild type and saccharomyces cerevisiae x2180-1 a-5 mutant strains by the action of zymolyase-60,000.the viable whole cells of saccharomyces cerevisiae x2180-1a wild type and its mannan mutant strain s. cerevisiae x2180-1a-5, were treated with an arthrobacter sp. beta-1,3-glucanase in the presence of a serine protease inhibitor, phenyl-methylsulfonyl fluoride. fractionation of the solubilized materials of each strain with cetavlon (cetyltrimethylammonium bromide) yielded one mannan-protein complex. molecular weights of these complexes were almost the same as that of the mannoprotein of the muta ...19836355061
use of specialized microbial strains in the treatment of industrial waste and in soil decontamination. 19836357840
detoxification of pesticides by microbial enzymes. 19836357841
[immobilization of cells with steroid-1,2-dehydrogenase activity on different carriers]. 19846377834
purification and properties of n-acetylneuraminate lyase from escherichia coli.n-acetylneuraminate lyase [n-acetylneuraminic acid aldolase ec 4.1.3.3] from escherichia coli was purified by protamine sulfate treatment, fractionation with ammonium sulfate, column chromatography on deae-sephacel, gel filtration on ultrogel aca 44, and preparative polyacrylamide gel electrophoresis. the purified enzyme preparation was homogeneous on analytical polyacrylamide gel electrophoresis, and was free from contaminating enzymes including nadh oxidase and nadh dehydrogenase. the enzyme c ...19846389524
immunochemical properties of mannan-protein complex isolated from viable cells of saccharomyces cerevisiae 4484-24d-1 mutant strain by the action of zymolyase.viable cells of saccharomyces cerevisiae 4484-24d-1 mutant strain were treated with an arthrobacter sp. beta-1,3-glucanase, zymolyase-60,000, in the presence of a serine protease inhibitor, phenylmethylsulfonyl fluoride. fractionation of the solubilized materials with cetavlon (cetyltrimethylammonium bromide) yielded a purified mannan-protein complex, which had a molecular weight of ca. 150,000, approximately three times higher than that of the mannan isolated from the same cells by the hot-wate ...19846398395
[taxonomic position of the genus mycococcus].an attempt to elucidate the taxonomic position of the genera mycococcus has been made. for this purpose the full range of the taxonomically important properties were investigated among microbial cultures preliminary identified as mycococci. as a result of comparison mycococcus with micrococcus the taxonomic status of the genus mycococcus was no longer confirmed. on the grounds of the published data and the author's experimental results it was concluded that genus micrococcus was closely related ...19836405816
purification and properties of a nad-linked 1,2-propanediol dehydrogenase from propane-grown pseudomonas fluorescens nrrl b-1244.nad-dependent 1,2-propanediol dehydrogenase (ec 1.1.1.4) activity was detected in cell-free crude extracts of various propane-grown bacteria. the enzyme activity was much lower in 1-propanol-grown cells than in propane-grown cells of pseudomonas fluorescens nrrl b-1244, indicating that the enzyme may be inducible by metabolites of propane subterminal oxidation. 1,2-propanediol dehydrogenase was purified from propane-grown ps. fluorescens nrrl b-1244. the purified enzyme fraction shows a single-p ...19836407398
identification key for coryneform bacteria derived by numerical taxonomic studies.six main groups were formed from a complete linkage dendrogram on 557 bacteria tested for 53 physiological features. the organisms were obtained from culture collections and included representatives of the following genera: arthrobacter, brevibacterium, caseobacter, cellulomonas, corynebacterium, curtobacterium, micrococcus, microbacterium, mycobacterium, nocardia, oerskovia and rhodococcus. the six groups were individually subjected to a numerical taxonomic analysis based on linkage maps, which ...19836413644
biology of aerobic carbon monoxide-oxidizing bacteria. 19836416144
microbial degradation of synthetic organochlorine compounds. 19836416886
retrohydroxamate ferrichrome, a biomimetic analogue of ferrichrome.a new synthetic analogue of ferrichrome, retrohydroxamate ferrichrome, has been examined for biological activity. although spectroscopic evidence indicates that the analogue is a weaker fe(iii) chelator than ferrichrome, retrohydroxamate ferrichrome is indistinguishable from ferrichrome in its growth factor activity for arthrobacter flavescens, and in its potency in antagonizing the antibiotic activity of albomyhcin against bacillus subtilis. it is as active as ferrichrome as a siderophore for t ...19846424674
the inactivation of glucosamine synthetase from bacteria by anticapsin, the c-terminal epoxyamino acid of the antibiotic tetaine.incubation of anticapsin with the purified glucosamine synthetase (2-amino-2-deoxy-d-glucose-6-phosphate ketol-isomerase, amino transferring, ec 5.3.1.19) from escherichia coli, pseudomonas aeruginosa, arthrobacter aurescens and bacillus thuringiensis led to the formation of an inactive enzyme irreversibly modified. the inactivation reaction followed pseudo-first-order kinetics. the rate of the inactivation reaction at various concentrations of anticapsin exhibited saturation kinetics, implying ...19846426523
[growth kinetics of microorganisms with various ecological strategies in a dialysis culture at low specific growth rates].a dialysis culture was found to be most suitable for studying the metabolism of microorganisms at a low specific growth rate. the biomass of all microorganisms studied in the dialysis culture increased with time in a linear fashion; hence, the energy spent for growth decreased in proportion to a decrease in the specific growth rate. microorganisms growing in oligotrophous environment (arthrobacter globiformis and lipomyces tetrasporus ) spent much less energy comparing to microorganisms from eut ...19846429490
inhibition of glucosamine-6-phosphate synthetase from bacteria by anticapsin.on the basis of kinetic studies on glucosamine-6-phosphate synthetase (ec 5.3.1.19) from six bacteria sources it has been shown that the epoxyamino acid anticapsin, a glutamine analog, is a competitive inhibitor of the enzyme in regard to glutamine with km value of 10(-4) m and ki varying from 10(-7) to 10(-6) m. unlike other glutamine analogs like 6-diazo-5-oxo-l-norleucine, chloropentanoic acid, l-alpha-amino-3-chloro-4,5-dihydro-5-isoxazole acetic acid or albizziin, anticapsin is not generall ...19846430854
[the phosphoketolase system of carbohydrate cleavage in saprophytic mycobacteria]. 19836435980
examination of protein sequence homologies: i. eleven escherichia coli l7/l12-type ribosomal "a" protein sequences from eubacteria and chloroplast.seven complete and four partial sequences of escherichia coli l7/l12-type ribosomal "a" proteins obtained from various bacteria (e. coli, bacillus subtilis, micrococcus lysodeikticus, rhodopseudomonas spheroides, desulfovibrio vulgaris, streptomyces griseus, bacillus stearothermophilus, clostridium pasteurianum, arthrobacter glacialis, and vibrio costicola) and spinach chloroplast have been reexamined using a computer program that searches for homologous tertiary structures. comparison matrices ...19846443314
[change in the microbiol complexes of soddy podzol soil under the influence of long-term spring wheat monoculture].the special composition and the properties of microorganisms predominant in the soil and in the rhizosphere of summer wheat were studied in the conditions of monoculture and corp rotation. noticeable differences were found in the composition of microbial complexes: microorganisms belonging to the genus arthrobacter prevailed in the conditions of corp rotation, whereas cultures of the genera pseudomonas and bacillus predominated in the conditions of monoculture. many of the latter possessed weak ...19806451787
production and isolation of siderophores from the soil fungus epicoccum purpurascens.a large number of iron transport agents, siderophores, which stimulated the growth of arthrobacter flavescens jg-9, were isolated during a study of the antitumor activity associated with the metabolic products of the fungus epicoccum purpurascens. the production of the siderophores was significantly enhanced in a variety of media by culture of the fungus in the near absence of ferric iron. a novel method of purification involving a carboxylic ion-exchange resin separated the siderophores into fo ...19816453608
siderophore production by pathogenic neisseria spp.previous studies have established the importance of iron acquisition to the growth and virulence of neisseria meningitidis and neisseria gonorrhoeae. although preliminary evidence that the neisseria spp. produce siderophores has been presented, the exact mechanism of iron acquisition has remained obscure. siderophore production by n. gonorrhoeae and n. meningitidis was induced in two different low-iron media. the iron-reactive siderophores, "gonobactin" and "meningobactin," were partially purifi ...19816454659
specificity and mechanism of ferrioxamine-mediated iron transport in streptomyces pilosus.although the ferrioxamines are an important and well-characterized class of siderophores produced by several species of nocardia, streptomyces, micromonospora, arthrobacter, chromobacterium, and pseudomonas, no studies of the mechanism of ferrioxamine-mediated iron uptake have been performed for an organism which produces the siderophore. this is the first report of metal transport in streptomyces pilosus mediated by the native ferrioxamines b, d1, d2, and e. 55fe accumulation in these ferrioxam ...19846480557
interaction between rat peritoneal macrophages and sialidase-treated erythrocytes: biochemical and morphological studies.rat peritoneal macrophages bind and phagocytose homologous, sialidase-treated erythrocytes at a rate depending on the number of red cells and the amount of sialic acids released. vibrio cholerae sialidase only partially (75%) removes the sialic acid residues from rat erythrocytes, whereas with arthrobacter ureafaciens sialidase complete desialylation is possible. analysis of the sialic acids by capillary gas-liquid chromatography combined with mass spectrometry (glc-ms) revealed the occurrence o ...19846487288
the origin of the oxygen incorporated during the dehalogenation/hydroxylation of 4-chlorobenzoate by an arthrobacter sp.an arthrobacter sp. has been shown to dehalogenate 4-chlorobenzoate yielding 4-hydroxybenzoate. experiments with 18o indicate that, in the presence of cell-free extracts, the hydroxyl group which is substituted onto the aromatic nucleus during dehalogenation is derived from water and not from molecular oxygen. dehalogenation therefore is not catalysed by a mixed-function oxidase; instead a novel aromatic hydroxylase is implicated in the reaction.19846497895
[microbiological studies of ground water polluted with hydrocarbons. 1. quantitative and qualitative distribution of bacterial populations].the groundwater, polluted with hydrocarbons, showed large cell numbers during one year of examination: 10(3)-10(4) cfu/ml grown on n-agar after 5 days at 20 degrees c, 10(4)-10(5) cfu/ml grown on water-agar after 8 weeks at 20 degrees c and total cell numbers of 10(5)-10(7)/ml. the bacteria, which were isolated from n-agar, were chiefly pseudomonas, flavobacterium, acinetobacter, and other gramnegative species, arthrobacter and bacillus as members of grampositive bacteria. furthermore the follow ...19846524146
purification of the flavoproteins 6-hydroxy-d- and 6-hydroxy-l-nicotine oxidase using hydrophobic affinity chromatography.a systematic study of the homologous series of omega-aminoalkyl-agaroses revealed differences in the affinities of 6-hydroxy-d- and 6-hydroxy-l-nicotine oxidase. in contrast to supports with nonpolar alkyl chains, omega-aminoalkyl-agarose showed high affinity towards the l-specific enzyme, while the d-specific oxidase was bound most firmly by omega-aminododecyl-agarose. 6-hydroxy-l-nicotine oxidase could be desorbed by 1.3m nacl only in the presence of the substrate l-6-hydroxynicotine. using th ...19836618442
[detection of teichoic acids in cells of bacteria of the genus arthrobacter]. 19836628186
effect of growing conditions on protein content and amino acid composition of arthrobacter simplex. 19836629843
[coupling between 3-ketosteroid-delta'-dehydrogenase and the respiratory chain in the bacterium arthrobacter globiformis].intact bacterial cells and their cytoplasmic membranes obtained by ultrasonic desintegration are able to induce dehydration of hydrocortisone to prednisolone that is coupled with o2 uptake. this reaction is inhibited by cyanide and 2-n-nonyl-4-hydroxyquinoline-n-oxide (hoqno). oxidation of hydrocortisone results in cytochrome reduction both in intact cells and in cytoplasmic membranes. aerobic dehydration of hydrocortisone is linked with transmembrane potential generation. the data obtained sugg ...19836639991
binding and phagocytosis of sialidase-treated rat erythrocytes by a mechanism independent of opsonins.rat peritoneal macrophages bind and phagocytoze homologous sialidase-treated erythrocytes at a rate which is dependent on the amount of sialic acid that has been removed from the cells. increased binding of erythrocytes is observed after the removal of 10-20% of membrane sialic acid, while for phagocytosis at least 30-40% of this substance must be removed. with vibrio cholerae sialidase only a partial (80%) hydrolysis of rat erythrocyte sialic acid is possible, whereas arthrobacter ureafaciens s ...19836642429
action of levan fructotransferase of arthrobacter ureafaciens on levanoligosaccharides.levan fructotransferase of the bacterium arthrobacter ureafaciens, which produces di-d-fructose 2,6':6,2' dianhydride (difructose anhydride iv) from levan by an intramolecular transfructosylation reaction, was purified to give a single protein band of pi 4.5-4.7 on isoelectric focusing. it had a molecular weight of 128,000 on gel-filtration on sephadex g-200 and 60,000 on sds-polyacrylamide disc gel-electrophoresis, suggesting that the enzyme is composed of two identical subunits. the shortest l ...19836654872
immobilization of proteins on partially hydrolyzed agarose beads.treatment of agarose beads with mild acid (0.2 m hcl, 55 degrees c, several hours) hydrolyzes some of the glycosidic bonds between d-galactosyl residues and 3,6-anhydro-l-galactosyl residues, and thus produces aldehydo-groups useful for immobilization of amino compounds by reductive amination with nacnbh3. more than 20 mg (0.3 mumol) of bovine serum albumin could be coupled per gram of partially hydrolyzed agarose beads. arthrobacter neuraminidase immobilized by this method was useful for desial ...19836660514
[sialidase from arthrobacter ureafaciens]. 19846718750
isolation and partial characterization of plasmid dna from arthrobacter oxidans.a method for the extraction of the high molecular weight plasmid ao 1 from the gram-positive soil bacterium arthrobacter oxidans is presented. following digestion of this dna with the restriction endonucleases acci, bam hi, eco ri and hind iii, an average molecular mass of 157.8 kb was estimated. this value is in good agreement with the 160 kb size determined previously by electron microscopy (brandsch et al. 1982). using the same method, no plasmid dna was found in strains of the genus arthroba ...19846742952
factors limiting the microbial conversion of sterols to 17-ketosteroids in the presence of metal chelate inhibitors.bioconversion of sterols to 17-ketosteroids by an arthrobacter species occurred in the presence of hydrophobic metal-chelating agents but the production of 17-ketosteroids (17-ks) was seriously limited by the rapid loss of the viability of cells in the presence of these inhibitors. besides, the conversion was inhibited by 17-ks at concentrations of 500 ppm or more. the 17-ks formed consisted exclusively of 1,4-androstadiene-3,17-dione (add) and 4-androstene-3,17-dione (ad) and these were found i ...19846745816
purification and characterization of beta-galactoside (alpha 2 leads to 6)sialyltransferase from rat liver and hepatomas.asialofetuin sialyltransferase from triton x-100 extracts of rat liver was resolved by phosphocellulose chromatography into two fractions, designated i and ii in order of elution. when previously treated with arthrobacter ureafaciens neuraminidase, fraction i eluted at about the same position as ii while no alteration occurred in ii. primary rat hepatomas contained only a single asialofetuin sialyltransferase, identical to fraction i in chromatographic behavior. transferases i and ii were purifi ...19826751821
factors determining steric course of enzymic glycosylation reactions: glycosyl transfer products formed from 2,6-anhydro-1-deoxy-d-gluco-hept-1-enitol by alpha-glucosidases and an inverting exo-alpha-glucanase.glycosyl transfer products were formed from 2,6-anhydro-1-deoxy-d-gluco-hept-1-enitol (heptenitol) by purified alpha-glucosidases from candida tropicalis and rice and by an inverting exo-alpha-glucanase (glucodextranase) from arthrobacter globiformis. the products were structurally defined through 1h and 13c nmr (nuclear magnetic resonance) spectra of their crystalline per-o-acetates in comparison with those of authentic methyl 1-deoxy-alpha- and methyl 1-deoxy-beta-d-gluco-heptuloside. 1-deoxy- ...19826758847
[microbiological transformation of hydrocortisone by free and immobilized cells]. 19826763460
a critical analysis of kinetic data of 3-hexulosephosphate synthases. michaelis-menten or complex characteristics.investigations of the 3-hexulosephosphate synthase (hps) from different methylotrophic bacteria have revealed apparent discrepancies in kinetic behaviour. in all methanol-utilizing species investigated by us the kinetic characteristics showed intermediary plateau regions. therefore, this behaviour is assumed to be a general feature of the hps from all non-methane-utilizing methylotrophic bacteria. however, this assumption is in contrast to the results of other authors. both for methylomonas m15 ...19806775423
[sulfur and iron cycling bacteria in low-sulfate meromictic lake kuznechikha].the hydrochemical characteristics, the composition of species and the localization of bacterial species involved in oxidation of sulfide and ferrous salts were studied in the meromictic lake kuznechikha with a low sulfate content in summer and in winter. the rate of bacterial sulfate reduction and the rates of bacterial and algal photosynthesis were determined using a radioisotope technique. the meromictic nature of the lake is due to the accumulation of ferrous salts (up to 212 mg/l fe2+) in th ...19806777648
radioimmunoassay of arthrobacter neuraminidase: applications to catalytic and taxonomic studies. 19806779664
isolation and structural characterization of human lymphocyte and neutrophil gangliosides.gangliosides were isolated from purified preparations of human peripheral blood lymphocytes and neutrophils. structural analyses and comparisons were performed by direct probe mass spectrometry and by degradation studies with the following enzymes: escherichia freundii endo-beta-galactosidase; clostridium perfringens and arthrobacter ureafaciens neuraminidase; and jack bean beta-n-acetylhexosaminidase and beta-galactosidase. this combination of techniques allowed us to obtain carbohydrate compos ...19816780555
[ratio of coryneform bacteria to the organic substance concentration].the effect of various organic substances and their concentrations on growth was studied with 10 strains of coryneform bacteria belonging to different taxonomic groups. the dynamics of the cultural growth depended on the nature of a substrate, glucose or acetate. arthrobacter globiformis 281, a. variabilis 289, nocardia erythropolis 236, n. globerula 502, n. minima 311 and n. rhodochrous 435 had a higher growth rate on acetate than on glucose. a. pascens 284, brevibacterium ammoniagenes 334, cory ...19806782432
scope and mechanism of carbohydrase action. stereocomplementary hydrolytic and glucosyl-transferring actions of glucoamylase and glucodextranase with alpha- and beta-d-glucosyl fluoride.rhizopus niveus glucoamylase and arthrobacter globiformis glucodextranase, which catalyze the hydrolysis of starch and dextrans, respectively, to form d-glucose of inverted (beta) configuration, were found to convert both alpha- and beta-d-glucosyl fluoride to beta-d-glucose and hydrogen fluoride. each enzyme directly hydrolyzes alpha-d-glucosyl fluoride but utilizes th beta-anomer in reactions that require 2 molecules of substrate and yield glucosyl transfer products which are then rapidly hydr ...19816787047
cell wall composition in the classification and identification of coryneform bacteria. 19806792712
[differentiation of the genera of coryneform bacteria synthesizing amino acids and nucleotides].the morphological, cultural, physio-biochemical and chemotaxonomic properties as well as the content of gc in dna were studied in coryneform bacterial strains producing amino acids and nucleotides. it has been shown that brevibacterium ammoniagenes vkm 672, b. flavum 317a, b. stationis ccm 317 and corynebacterium vsti 301 should be assigned to the genus corynebacterium. the taxonomic significance of chemotaxonomic and physio-biochemical properties is discussed on the basis of the results obtaine ...19826803106
numerical classification of some rhodococci, corynebacteria and related organisms.nineteen strains of corynebacterium sensu stricto, 23 received as corynebacterium equi or rhodococcus equi, marker cultures of arthrobacter, brevibacterium, bacterionema matruchotii, cellulomonas flavigena, kurthia zopfii, listeria denitrificans, microbacterium lacticum, rhodococcus rubropertinctus and 88 representatives of mycobacterium, nocardia, rhodococcus and the 'aurantiaca' taxon were the subject of numerical phenetic analyses using 92 characters. the data were examined using the simple m ...19826811696
involvement of plasmids in total degradation of chlorinated biphenyls.acinetobacter sp. strain p6 has previously been reported to utilize biphenyl (bp) and chlorinated bps, with accumulation of corresponding chlorobenzoic acids. arthrobacter sp. strain m5 was isolated as a contaminant in the culture of acinetobacter sp. strain p6 growing on 4-chlorobiphenyl and showed properties similar to p6 in the degradation of chlorinated bps. both strains harbored an identical plasmid of 53.7 megadaltons. these strains spontaneously lost the ability to utilize bp and 4-chloro ...19826814360
the interaction of substrate-related ketals with bacterial and viral neuraminidases.arthrobacter sialophilus neuraminidase catalyzes the hydration of 5-acetamido-2,6-anhydro-3,5-dideoxy-d-glycero-d-galacto-non-2-enonic acid (2,3-dehydro-acneu) with km and kcat values of 8.9 x 10(-4) m and 6.40 x 10(-4) s-1, respectively. the methyl ester of 2,3-dehydro-acneu as well as 2,3-dehydro-4-epi-acneu are also hydrated by the enzyme. the product resulting from the enzymatic hydration of 2,3-dehydro-acneu is n-acetylneuraminic acid. a series of derivatives of 2,3-dehydro-acneu (k1, 1.60 ...19836830254
studies on isoprenoid biosynthesis with bacterial intact cells.for the study on the regulation of isoprenoid biosynthesis with intact cells, some strains of bacteria capable of growing on mevalonate as a sole carbon source were isolated from soil. many of them incorporated [14c]-mevalonate, [14c]isopentenyl- and [14c]farnesyl pyrophosphates into the cells. however, radioactivity was found in their degradation products but not in isoprenoids. addition of [14c]isopentenyl pyrophosphate, farnesyl pyrophosphate and mg2+ ions in combination to the culture of a s ...19836838508
[dynamics of populations of microbial antagonists in nonsterile soil].the interaction between populations was studied with arthrobacter crystallopoietes and streptomyces olivocinereus, an actinomycete producing the antibiotic heliomycin active against gram-positive microorganisms. the two organisms were either cultivated together in a growth medium or the two populations were introduced simultaneously into nonsterile soil at different levels of population density. the antagonism was found in both cases: a. crystallopoietes cells died off when a population of the p ...19836843384
similarities in the induction of synthesis of a cell-surface polypeptide in arthrobacter sp. by near-uv irradiation and photodynamic conditions. 19836856688
quantitation of immunoadsorbed flavoprotein oxidases by luminol-mediated chemiluminescence.the detection of the flavoenzymes 6-hydroxy-l-nicotine oxidase and 6-hydroxy-d-nicotine oxidase at the sub-femtomol level was achieved by coupling the reaction of the immunoadsorbed proteins to the peroxidase-catalysed oxidation of luminol. the h2o2-producing oxidases retained their full activity when bound to the respective immobilized antibodies. this fact allowed the concentration of the enzymes from very dilute solutions and the quantitative assay of their activities in the microu range. due ...19836862382
[regulation of citrate synthase in facultative methylotrophic bacteria].commonly the tca cycle fulfils an anabolic and a catabolic function in case of aerobic chemoorganoheterotrophic nutrition. in methylotrophic growth the tca cycle is dispensable as a bioenergetic pathway. this is reflected by properties of citrate synthase in facultative methylotrophic bacteria. two citrate synthases, a "chemoorganoheterotrophic" one, which is inhibited by nadh (or atp in acetobacter mb 58), and a "methylotrophic" one, which is not or less affected by energy indicators, were foun ...19836880250
a taxonomic study of gardnerella vaginalis (haemophilus vaginalis) gardner and dukes 1955.fifty-five strains received as haemophilus vaginalis or as catalase-negative coryneform bacteria from the vagina together with 61 marker cultures were subjected to numerical phenetic analyses using 149 unit characters. the data were examined using the simple matching (ssm), jaccard (sj) and pattern (dp) coefficients and clustering was achieved using the average linkage algorithm. cluster composition was not markedly affected by the coefficient used or by test error, estimated at 6 . 5%. the h. v ...19806971916
cell-free synthesis of a flavoprotein containing the 8 alpha-(n3-histidyl)-riboflavin linkage.6-hydroxy-d-nicotine oxidase is an inducible flavorprotein of arthrobacter oxidans in which one mole of fad is bound covalently to the polypeptide chain. during cell-free translation of polysomes from nicotine-induced a. oxidans cells in the presence of an escherichia coli (mre 600) supernatant fraction, labelled fad, leucine and histidine were incorporated into 6-hydroxy-d-nicotine oxidase in the same ratio found in the enzyme isolated from whole cells. this indicates that one mole fad is coval ...19806988214
evolutionary relationship between halobacterium cutirubrum and eukaryotes determined by use of aminoacyl-trna synthetases as phylogenetic probes.the cross-species reactivities between trnas and aminoacyl-trna synthetases have been employed as a basis to estimate the relatedness of various prokaryotes to the eukaryotes. the trna of halobacterium cutirubrum, unlike that of other prokaryotes tested, including agrobacterium tumefaciens, arthrobacter luteus, bacillus subtilis, bacillus stearothermophilus, escherichia coli, micrococcus luteus, myxococcus xanthus, rhodopseudomonas spheroides, and thermus aquaticus, was found to share with yeast ...19806989454
[superoxide dismutase--radiobiologic significance and possibilities (review)].modern data are considered on the importance of superoxide radical in the oxygen effect of ionizing radiation as well as the participation of superoxide dismutase in biological radioresistance. biological role and some physico-chemical properties of the enzyme are described. estimation of the superoxide dismutase activity might serve as a test for study of the primary effect of ionizing radiation on cells; at the same time, the enzymatic preparations could be used as radioprotective drugs.19807006203
a new enzymatic method for the determination of sialic acid in serum and its application for a marker of acute phase reactants. 19817021999
microbial adaptations to stress: some lessons to be learned from aerobes. 19817023462
an efficient technique for the isolation of yeast spores and the preparation of spheroplast lysates active in protein synthesis. 19817032354
[hydrocortisone transformation into prednisolone by microorganisms]. 19807041529
[factors increasing exoprotease activity in the associative cultures of coryneform bacteria].specific features of the effect of artrobacter citreus on the biosynthesis of fibrinolytic enzymes of nocardia sp. 1 were investigated during the combined cultivation of both microorganisms. it was demonstrated that an increase in the synthesis of fibrinolytic proteases in the mixed culture of a. citreus and n. species 1 was determined by the compounds containing in the a. citreus supernatant rather than by the nutrient deficiency.19827043443
oxygen tolerance of strictly aerobic hydrogen-oxidizing bacteria.growth of various bacteria, especially aerobic hydrogen-oxidizing bacteria, in the presence of 2 to 100% (v/v) oxygen in the gas atmosphere was evaluated. the bacterial strains included alcaligenes eutrophus, a. paradoxus, aquaspirillum autotrophicum, arthrobacter spec. strain 11 x, escherichia coli, arthrobacter globiformis, nocardia opaca, n. autotrophica, paracoccus denitrificans, pseudomonas facilis, p. putida, and xanthobacter autotrophicus. under heterotrophic conditions with fructose or g ...19827049081
contractile effects of bacterial cell walls, their enzymatic digests, and muramyl dipeptides on ileal strips from guinea pigs.cell walls isolated from four bacterial species (streptococcus pyogenes, lactobacillus plantarum, streptomyces gardneri, and nocardia corynebacteriodes), which exhibited the adjuvant effect of stimulating cellular and humoral immune responses against ovalbumin in guinea pigs, caused the slow-starting and long-lasting contraction of guinea pig ileal strips suspended in tyrode solution. in contrast to these cell walls active in immunoadjuvancy, those isolated from five bacterial species (micrococc ...19827056578
substrate and product specificity of arthrobacter sialophilus neuraminidase.arthrobacter sialophilus neuraminidase catalyzes the hydrolysis of n-acetylneuraminyl-alpha-oxygen, nitrogen, and azido glycosides. the most effective of those substrates examined was n-acetylneuraminyl-alpha-4-methylumbelliferylglycoside (acneu-alpha-4-mu; km app, 0.0193 mm; kcat, 136.4 sec-1). the products resulting from the enzymic hydrolysis of n-acetylneuraminyl-alpha-azido-glycoside were n-acetylneuraminic acid and azide ion. n-acetylneuraminyl-alpha-2,3-thiogalactylglycoside and n-acetyln ...19827068676
metabolism of cyclohexaneacetic acid and cyclohexanebutyric acid by arthrobacter sp. strain ca1.a strain of arthrobacter was isolated by enrichment culture with cyclohexaneacetate as the sole source of carbon and grew with a doubling time of 4.2 h. in addition to growing with cyclohexaneacetate, the organism also grew with cyclohexanebutyrate at concentrations not above 0.05%, and with a variety of alicyclic ketones and alcohols. oxidation of cyclohexaneacetate proceeded through formation of the coenzyme a (coa) ester followed by initiation of a beta-oxidation cycle. beta-oxidation was blo ...19827076617
microbial transformations of warfarin: stereoselective reduction by nocardia corallina and arthrobacter species.the microbiological metabolism of warfarin was examined as a model of metabolism in higher organisms, including humans, and to determine the chirality of microbial reductases for application in organic synthesis. nineteen cultures were examined based on their reported abilities to reduce ketonic substrates, and several were shown to catalyze the desired reaction. nocardia corallina (atcc 19070) exhibited complete substrate and product stereoselectivity as it reduced s-warfarin to the correspondi ...19827081986
a survey of the structures of mycolic acids in corynebacterium and related taxa.acid methanolysates of 104 strains of coryneform and related bacteria were examined for mycolic acids by thin-layer chromatography and mass spectrometry. the mycolic acids of corynebacterium sensu stricto strains were not uniform in size and structure, and considerable variation existed within the approximate limiting range of c22 to c36. two species, corynebacterium bovis and 'corynebacterium mycetoides', were particularly distinctive; the former had exceptionally low molecular weight mycolic a ...19827086391
a preferential isolation procedure for asporogenous gram-positive bacteria.a preferential isolation procedure was devised for asporogenous (asp), gram-positive (gp), aerobic or facultative anaerobic bacteria which included the genera arthrobacter, corynebacterium, brevibacterium, microbacterium, mycobacterium, and micrococcus (asp-gp bacteria). an antibiotics-mixture agar which contained 5 to 10 micrograms per ml of colistin, 10 to 20 micrograms per ml of nalidixic acid and 30 micrograms per ml of cycloheximide was used in the isolation. using this technique 47 asp-gp ...19827096199
microbiological examination of sebeel water.water samples from clay storage jugs ("zeers") located in homes and at public watering stands ("sebeels") at streets, mosques, and schools were examined. coliforms, fecal coliforms, and fecal streptococci were detected in 100, 69, 88, and 91.56% of the samples, respectively. the general microbiology of the water and some factors affecting microbial load were studied. the predominant bacterial genera of sebeel water were found to be staphylococcus. aerococcus, micrococcus, streptococcus, bacillus ...19827103483
structural requirements for neuraminidase induction in arthrobacter sialophilus.the effectiveness of 13 n-acetylneuraminic acid derivatives as potential inducers of arthrobacter sialophilus neuraminidase were examined. n-acetylneuraminic acid nitrogen and thioglycosides were not inducers, whereas 2,3-dehydro-n-acetylneuraminic acid, a transition state analog for neuraminidases, was the most effective inductive ligand. the c-4 hydroxyl function of n-acetylneuraminic acid was essential for enzyme derepression.19827107565
lack of ultraviolet mutagenesis in radiation-resistant bacteria.ultraviolet (uv) radiation did not induce rifampicin-resistant mutants in populations of the taxonomically-related radiation-resistant bacteria deinococcus radiodurans, d. radiopugnans, d. radiophilus and d. proteolyticus, although such mutants arose spontaneously at a low frequency and at a high frequency after treatment of cultures with n-nitroso compounds. the radiation-resistant bacteria arthrobacter radiotolerans and p-30-a were also uv-immutable whereas the more radiation-sensitive pseudom ...19827110166
chlorine resistance patterns of bacteria from two drinking water distribution systems.the relative chlorine sensitivities of bacteria isolated from chlorinated and unchlorinated drinking water distribution systems were compared by two independent methods. one method measured the toxic effect of free chlorine on bacteria, whereas the other measured the effect of combined chlorine. bacteria from the chlorinated system were more resistant to both the combined and free forms of chlorine than those from the unchlorinated system, suggesting that there may be selection for more chlorine ...19827149722
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