molecular cloning of the penicillin g acylase gene from arthrobacter viscosus.penicillin g acylase was purified from the cultured filtrate of arthrobacter viscosus 8895gu and was found to consist of two distinct subunits with apparent molecular weights of 24,000 (alpha) and 60,000 (beta). the partial n-terminal amino acid sequences of the alpha and beta subunits were determined with a protein gas phase sequencer, and a 29-base oligonucleotide corresponding to the partial amino acid sequence of the alpha subunit was synthesized. an escherichia coli transformant having the ...19883214149
oxidative side-chain and ring fission of pregnanes by arthrobacter simplex.metabolic processes involving side-chain and ring cleavage of progesterone, 17-hydroxyprogesterone, 11-deoxycortisol and 16-dehydropregnenolone by arthrobacter simplex were studied. the formation of the metabolites from progesterone indicates a pathway somewhat different from normal in the enzymic reaction sequence, and the 17-hydroxyprogesterone metabolites reveal a non-enzymic rearrangement step. the presence of a hydroxy group at c-21, as in 11-deoxycortisol, induces reduction of the c-20 car ...19883214423
three dehalogenases and physiological restraints in the biodegradation of haloalkanes by arthrobacter sp. strain ha1.arthrobacter sp. strain ha1 utilizes 18 c2-to-c8 1-haloalkanes for growth and synthesizes an inducible 1-bromoalkane debrominase of unknown physiological function (r. scholtz, t. leisinger, f. suter, and a.m. cook, j. bacteriol. 169:5016-5021, 1987) in addition to an inducible 1-chlorohexane halidohydrolase which dehalogenates some 50 substrates, including alpha, omega-dihaloalkanes. alpha, omega-dihaloalkanes were utilized by cultures of strain ha1 under certain conditions only. c9 and c8 homol ...19883223767
enzymatic dehalogenation of 4-chlorobenzoate by extracts from arthrobacter sp. su dsm extracts from arthrobacter sp. su dsm 20407 an enzyme was detectable, that converted 4-chlorobenzoate into 4-hydroxybenzoate. this conversion was also observed when no oxygen was present in the reaction mixture. boiling for 5 min destroyed the enzyme activity. 4-bromo- and 4-iodobenzoate were substrates for the enzyme too, but not 4-fluorobenzoate, 4-chlorophenylacetate and 4-chlorocinnamic acid. the enzyme showed optimum activity at 16 degrees c and at ph 7-7.5. the specific activity in the ...19883223988
degradation of 3-aminophenol by arthrobacter spec. ma3.the bacterial strain ma3 capable of utilizing 3-aminophenol as the sole source of carbon, energy and nitrogen for growth was isolated from an enrichment culture and identified as an arthrobacter species. utilization of 0.68 mg/ml 3-aminophenol by batch cultures of this organism was characterized by a specific growth rate (mu) of 0.18 h-1 and a yield coefficient (y) of 0.60. in chemostat cultures of strain ma3 we determined a critical dilution rate (dc) of 0.175 h-1 by continuous addition of mine ...19883236220
stereochemical studies of d-glucal hydration by alpha-glucosidases and exo-alpha-glucanases: indications of plastic and conserved phases in catalysis by glycosylases.alpha-glucosidases from aspergillus niger, pig serum, ungerminated rice, buckwheat, and sugar beet seeds (but not from brewers' yeast or honeybee) were found to catalyze the hydration of d-glucal. each reactive alpha-glucosidase, incubated with d-glucal in d2o, was shown to protonate (deuteriate) this prochiral substrate from above its re face, i.e., from a direction opposite that assumed for protonating alpha-d-glucosidic substrates. at the same time, d-glucal hydration catalyzed by three of th ...19883284583
induction of a light-inducible gene in arthrobacter sp. by exposure of cells to chelating agents and ph 5.transcription of a light-inducible gene in the prokaryote arthrobacter sp. is induced in the dark when cells are incubated with chelating agents or in medium at ph 5. however, repletion of metal ions such as ca2+, mn2+ or zn2+ or an increase in ph is required for accumulation of the gene product, an mr 21,000 polypeptide. but such changes in condition restore repression of the gene, and the decay in the rate of synthesis of the polypeptide follows the same time-course as when photodynamically in ...19883382667
bacterial flora in bottled uncarbonated mineral drinking water.a quantitative study of bacterial populations in mineral water was carried out. samples were stored at 6 and 20 degrees c, and the colony counts were determined on tryptone agar plates incubated at 22 and 37 degrees c. samples were collected from the spring source in sterile glass flasks and from the bottling factory in conventional plastic and glass containers. in both cases, the initial population (10(1)-10(2) cfu/ml water) increased to 10(5)-10(6) cfu/ml after 3 days storage as determined fro ...19873446349
comparison of backbone structures of glucose isomerase from streptomyces and arthrobacter.the c alpha backbones of the glucose isomerase molecules of streptomyces rubiginosus and arthrobacter have been determined by x-ray crystallography and compared. each molecule is a tetramer of eight-stranded alpha/beta barrels, and the mode of association of the tetramers is identical in each case. the arthrobacter electron density shows four additional amino acids at the carboxyl terminus. there is also an insertion of six amino acids at position 277, and two individual insertions at about posi ...19873508294
studies in vitro on the flavinylation of 6-hydroxy-d-nicotine oxidase.the gene of 6-hydroxy-d-nicotine oxidase (6-hdno), a flavoenzyme from arthrobacter oxidans with covalently bound fad, was expressed with the aid of an expression vector in a cell-free coupled transcription-translation system derived from escherichia coli mz9. ultraviolet irradiation of the e. coli extract did not affect synthesis of the 6-hdno polypeptide nor total protein synthesis but enzymatic 6-hdno activity could not be detected. addition of fad to the irradiated cell extract restored the c ...19863533536
enzymes of vitamin b6 degradation. purification and properties of pyridoxine 5'-dehydrogenase (oxidase).isolation and identification of a soil bacterium, arthrobacter cr-7, that grows with pyridoxine as a sole source of carbon and nitrogen are described. an inducible pyridoxine 5'-dehydrogenase (oxidase) (ec that catalyzes conversion of pyridoxine to isopyridoxal, pyridoxine + x----isopyridoxal + xh2, the first step in utilization of pyridoxine as a growth substrate by this organism, was purified about 520-fold to homogeneity. the enzyme (mr = 112,000) is a dimer of probably identical su ...19863533935
enzymes of vitamin b6 degradation. purification and properties of isopyridoxal dehydrogenase and 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylic-acid dehydrogenase.two nad+-dependent, highly specific pyridine-5-aldehyde dehydrogenases, 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylic-acid (compound 1) dehydrogenase and isopyridoxal dehydrogenase, were purified to homogeneity from pseudomonas ma-1 and arthrobacter cr-7, respectively. both enzymes are induced in response to growth of the organisms on pyridoxine and catalyze steps in the degradation of this compound by these organisms. compound 1 dehydrogenase (mr = 65,000) contains two subunits of equal size ...19863533936
[microbial transformation of 16 alpha-methyl-5 alpha-delta 9(11)- pregnene-3 beta, 17 alpha, 21-triol-20-one-3 beta, 21-diacetate]. 19863577788
microbiological and chemical dehydrogenation of withaferin a.arthrobacter simplex dehydrogenated withaferin a to 4-dehydrowithaferin a but it was not able to dehydrogenate this substrate in position 27. 27-dehydrowithaferin a was prepared chemically using pyridinium chlorochromate. whereas 4-dehydrowithaferin a surpassed in its effect on leukemic (388 cells the original compound and all its derivates synthesized so far, 27-dehydrowithaferin a was biologically inactive.19873583136
isolation and characterization of an s-ethyl-n,n-dipropylthiocarbamate-degrading arthrobacter strain and evidence for plasmid-associated s-ethyl-n,n-dipropylthiocarbamate degradation.arthrobacter sp. strain te1 isolated from s-ethyl-n,n-dipropylthiocarbamate (eptc)-exposed soil degraded this herbicide effectively and could grow on eptc as the sole carbon source. te1 harboured four plasmids of 65.5, 60, 50.5, and 2.5 megadaltons. spontaneous mutants unable to degrade eptc arose at a high frequency, and this was further increased by treatment of the culture with acridine orange or incubation at high temperature. all eptc degradation-deficient (e-) mutants lacked the 50.5-megad ...19873606092
[microbial transformation of 16 alpha-methyl-3 beta, 17 alpha-dihydroxy-5 alpha-pregnane-20-one-3 acetate]. 19873618239
6-hydroxy-d-nicotine oxidase of arthrobacter oxidans. gene structure of the flavoenzyme and its relationship to 6-hydroxy-l-nicotine oxidase.the nucleotide sequence of the 6-hydroxy-d-nicotine oxidase (6-hdno) gene of arthrobacter oxidans is presented. this covalently flavinylated enzyme specifically oxidizes 6-hydroxy-d-nicotine to 6-hydroxy-n-methylmyosmine. coinduced in the presence of nicotine is a 6-hydroxy-l-nicotine-specific enzyme, 6-hydroxy-l-nicotine oxidase (6-hlno), with fad noncovalently bound to the apoprotein. a comparison of the nucleotide-derived amino acid sequence of the 6-hdno with the amino acid sequence data obt ...19873622516
[kinetics of delta'-dehydrogenation of hydrocortisone by arthrobacter simplex by-2-13. i. kinetics of delta'-dehydrogenation of hydrocortisone by free cells of arthrobacter simplex by-2-13]. 19873630138
[microbial reduction of c20-keto of corticosteroids by arthrobacter 9-2. ii. bioconversion of 3 beta,17 alpha,21-triol-5 alpha-pregnan-3-one]. 19873630139
[kinetics of delta'-dehydrogenation of hydrocortisone by arthrobacter simplex by-2-13. ii. kinetics of delta'-dehydrogenation of hydrocortisone by immobilized a. simplex by-2-13]. 19873630142
novel mechanisms of resistance to lincosamides in staphylococcus and arthrobacter spp.clinical isolates of staphylococcus and arthrobacter spp. were screened for lincosamide resistance. six different patterns of resistance were found. strains designated sf27 and sf28 showed low-level resistance to lincosamides: one was susceptible to erythromycin (sf27) and the other was resistant (sf28). analysis of ribosomes from the resistant strains in an in vitro poly(u)-dependent protein-synthesizing system showed that ribosomes of both strains were sensitive to lincomycin and clindamycin. ...19883377455
[hydrocarbon-oxidizing microflora from the water of the baltic sea and kurshsky bay polluted after a fuel oil spill].microbiological studies were conducted in the water of the baltic sea and the kurshsky bay polluted with mazut as the result of a tanker wreck in november 1981 as well as in the water of nonpolluted regions. within the summer of 1982 and 1983, 755 bacterial strains were isolated from water samples taken at three different depths. bacteria belonging to the genera pseudomonas, rhodococcus + mycobacterium and arthrobacter predominated in the hydrocarbon-oxidizing cenoses of the baltic sea and the k ...19873657616
degradation 1,2-dimethylbenzene by corynebacterium strain an attempt to obtain bacteria growing on 1,2-dimethylbenzene as sole carbon and energy source two different strains were isolated. one was identified as an arthrobacter strain, the other as a corynebacterium strain. corynebacterium strain c125 was further investigated. the organism was not capable to grow on 1,3- and 1,4-dimethylbenzene. cis-1,2-dihydroxycyclohexa-3,5-diene oxidoreductase and 3,4-dimethylcatechol-2,3-dioxygenase activity was found in cell extracts. when 3,4-dimethylcatechol w ...19873662487
microbial desulfonation of substituted naphthalenesulfonic acids and benzenesulfonic acids.sulfur-limited batch enrichment cultures containing one of nine multisubstituted naphthalenesulfonates and an inoculum from sewage yielded several taxa of bacteria which could quantitatively utilize 19 sulfonated aromatic compounds as the sole sulfur source for growth. growth yields were about 4 kg of protein per mol of sulfur. specific degradation rates were about 4 to 14 mu kat/kg of protein. a pseudomonas sp., an arthrobacter sp., and an unidentified bacterium were examined. each desulfonated ...19873662502
characterization of 1-chlorohexane halidohydrolase, a dehalogenase of wide substrate range from an arthrobacter sp.1-chlorohexane halidohydrolase from arthrobacter sp. strain ha1 was purified to homogeneity by fractional precipitation, ion-exchange chromatography, gel filtration, and high-performance liquid chromatography gel filtration. the enzyme was a monomer with a molecular weight of about 37,000; its amino acid composition and n-terminal sequence were determined. the enzyme had a broad optimum around ph 9.5, a temperature optimum near 50 degrees c, an activation energy of 40 kj/mol, and a molecular act ...19873667524
activation of the human complement cascade by bacterial cell walls, peptidoglycans, water-soluble peptidoglycan components, and synthetic muramylpeptides--studies on active components and structural requirements.cell walls isolated from 29 strains of 24 gram-positive bacterial species, whose peptidoglycans belong to the group a type of schleifer and kandler's classification, with one exception (arthrobacter sp.), were shown to activate the complement cascade in pooled fresh human serum mainly through the alternative pathway and partly through the classical one. the complement-activating effect of cell walls (5 species) possessing group b type peptidoglycan, except those of corynebacterium insidiosum, wa ...19873670125
purification and properties of synephrinase from arthrobacter synephrinum.synephrinase, an enzyme catalyzing the conversion of (-)-synephrine into p-hydroxyphenylacetaldehyde and methylamine, was purified to apparent homogeneity from the cell-free extracts of arthrobacter synephrinum grown on (+/-)-synephrine as the sole source of carbon and nitrogen. a 40-fold purification was sufficient to produce synephrinase that is apparently homogeneous as judged by native polyacrylamide gel electrophoresis and has a specific activity of 1.8 mumol product formed/min/mg protein. ...19863729420
glycosaminoglycans in cortical autopsy samples from alzheimer brain.each of the known classes of mammalian glycosaminoglycans, with the exception of keratan sulphate, was found in cerebral cortex samples from patients with alzheimer-type dementia and age-matched controls. these molecules were quantitated, after electrophoresis and staining with alcian blue dye, by scanning densitometry. no significant differences were found between the mean levels of each of the above glycosaminoglycans in frontal cortex from patients with dementia compared with controls. an inc ...19883139840
[the use of bioelectrochemical systems in steroid biosynthesis].the feasibility of electrosynthesis involving the use of biocatalysts has been demonstrated in a model reaction of hydrocortisone oxidation to prednisolone catalyzed by arthrobacter globiformis cells. electrochemical regeneration of phenazine methosulfate, an artificial electron acceptor, was performed, using electrodes made of different materials. transformation products were analyzed by high performance liquid chromatography.19863768442
enzymes of vitamin b6 degradation. purification and properties of 4- and 5-pyridoxolactonases.4-pyridoxolactone and 5-pyridoxolactone, formed by dehydrogenation of pyridoxal or isopyridoxal during the bacterial degradation of vitamin b6 by pseudomonas ma-1 and arthrobacter cr-7, respectively, are hydrolyzed to the corresponding acids by distinct inducible lactonases which were purified to homogeneity. 4-pyridoxolactonase from pseudomonas ma-1 has an mr of 54,000 and contains two probably identical subunits of mr = 28,600. it has a ph optimum of 7.0, a km of 5.9 microm, and a vmax at 25 d ...19863771565
enzymes of vitamin b6 degradation. purification and properties of 5-pyridoxic-acid oxygenase from arthrobacter sp.5-pyridoxic-acid oxygenase, a cytoplasmic enzyme formed when arthrobacter cr-7 is grown with pyridoxine as a sole source of carbon and nitrogen, was purified about 190-fold to homogeneity from fully induced cells. the enzyme catalyzes reaction a, (formula: see text) the essential ring-opening step in the degradation of pyridoxine, and provides a second example of an fad-dependent oxygenase that adds both two hydrogen and two oxygen atoms to its substrate. 5-pyridoxic-acid oxygenase has an isoele ...19863771566
methylamine oxidase from arthrobacter p1. a bacterial copper-quinoprotein amine oxidase.methylamine oxidase from arthrobacter p1 was purified to homogeneity. the enzyme oxidizes primary amines but not tyramine or polyamines like spermine and putrescine. the enzyme activity has a ph optimum of 8.0 with methylamine, and is inhibited by certain cations as well as anions at rather low concentrations. the enzyme has an mr of 167900, an isoelectric point of 4.6, consists of two (probably identical) subunits (mr 82250) and contains two copper atoms but no sugar residues. the visible absor ...19863780750
surface localization of sialic acid on actinomyces viscosus.this study reports the presence of sialic acid in actinomyces viscosus strains t14v and t14av. mild acid hydrolysis of whole organisms released a compound which reacted positively in the periodate-thiobarbituric acid, direct ehrlich's and resorcinol assays, and which co-chromatographed on paper with authentic n-acetylneuraminic acid. strain t14v contained 10-fold greater concentrations of sialic acid than did strain t14av. sialic acid content was dependent upon the stage of growth of the culture ...19863655719
in vivo and in vitro expression of the 6-hydroxy-d-nicotine oxidase gene of arthrobacter oxidans, cloned into escherichia coli, as an enzymatically active, covalently flavinylated polypeptide.the 6-hydroxy-d-nicotine oxidase gene of arthrobacter oxidans was cloned into e.coli with the aid of the expression vector pkk223-3. this enzyme, as well as the e.coli enzymes succinate dehydrogenase and fumarate reductase, bears the cofactor fad covalently attached to the polypeptide through a his-n3-8 alpha-linkage. the amino acid sequence surrounding the histidine residue involved in fad binding in 6-hydroxy-d-nicotine oxidase and the two e.coli enzymes, however, show no homology. nevertheles ...19853905431
immunodiffusion and lipid analyses in the classification of "mycobacterium album" and the "aurantiaca" taxon.comparative immunodiffusion studies were performed on representative strains of "mycobacterium album", the "aurantiaca" taxon and an "aurantiaca"-like group of actinomycetes, using reference systems representing 25 taxa assigned to the genera arthrobacter, corynebacterium, kurthia, mycobacterium, nocardia, rhodococcus, streptomyces and the "aurantiaca" taxon. thin-layer chromatographic analyses of whole-organism methanolysates were carried out on single strains of "mycobacterium album" and the " ...19853923731
glutathione-independent isomerization of maleylpyruvate by bacillus megaterium and other gram-positive bacteria.maleylpyruvate, the ring fission product of gentisic acid, was found to be isomerized to fumarylpyruvate without a requirement for glutathione by an enzyme activity found in cell extracts of m-hydroxybenzoate-grown bacillus megaterium 410. the isomerization reaction was detected as a shift in the absorbance maximum from 330 nm, the maximum for maleylpyruvate, to 345 nm, the maximum for fumarylpyruvate, when assayed at ph 8.0. ammonium sulfate precipitation and dialysis of b. megaterium cell extr ...19853926749
growth of bacteria on chitin, fungal cell walls and fungal biomass, and the effect of extracellular enzymes produced by these cultures on the antifungal activity of amphotericin b.vibrio alginolyticus, streptomyces griseus, arthrobacter g12, bacillus sp. and cytophaga sp. were grown on solid and liquid media containing soluble and insoluble carbon sources. arthrobacter g12, bacillus sp. and cytophaga sp. grew well on media which contained fungal cell walls or fungal biomass as the main carbon source. all bacteria produced extracellular proteases and all bacteria except arthrobacter g12 produced extracellular chitinases. growth of cytophaga sp. on colloidal chitin was para ...19853929028
enzymes of vitamin b6 degradation. purification and properties of two n-acetylamidohydrolases.alpha-(n-acetylaminomethylene)succinic acid hydrolase (compound a hydrolase, ec 3.5.1-) and alpha-hydroxymethyl-alpha'-(n-acetylaminomethylene)succinic acid hydrolase (compound b hydrolase, ec 3.5.1-) were purified to homogeneity from pseudomonas ma-1 and arthrobacter cr-7, respectively. the two inducible enzymes catalyze reactions 1 and 2, respectively, which release the first generally useful anabolic intermediates during growth of these organisms with (formula; see text) pyridoxine as a sole ...19853972793
survival of brevibacterium linens during nutrient starvation and intracellular changes.the present work reports the survival capacity of a strain of brevibacterium linens isolated from a french camembert cheese and the ensuing changes in cell composition. exponentially growing cells were harvested, washed and resuspended with shaking in ph 8.0 buffer at 21 degrees c in the absence of a carbon source. the viability of this strain, assessed with slide cultures, is much less than that of coryneform bacteria isolated from soil samples, even though no cell lysis was detected. intracell ...19853994487
the cytotoxic and photodynamic inactivation of micro-organisms by rose bengal.rose bengal was cytotoxic to the following bacteria at the concentrations given in parentheses (highest concentrations of dye in mol/l at which growth occurred on nutrient medium): brochothrix thermosphacta and deinococcus radiodurans (1 x 10(-6) or less); streptococcus, micrococcus, staphylococcus, bacillus, arthrobacter and kurthia spp. (1 x 10(-5)-1 x 10(-4], and pseudomonas spp. and enterobacteriaceae (5 x 10(-3)-1 x 10(-2) or greater). these organisms were killed rapidly when suspended in i ...19853997691
localization of the enantiozymes of 6-hydroxy-nicotine oxidase in arthrobacter oxidans by electron immunochemistry.during the course of growth of arthrobacter oxidans, induction of the enantiozymes 6-hydroxy-d-nicotine oxidase and 6-hydroxy-l-nicotine oxidase occurred in the presence of dl-nicotine. cryoultramicrotomed sections obtained from cells grown to stationary phase were gold immunolabeled. the results obtained demonstrate that both enzymes are localized in the cytoplasm.19854019415
intermolecular fructosyl and levanbiosyl transfers by levan fructotransferase of arthrobacter ureafaciens.a purified levan fructotransferase preparation from the culture of the bacterium arthrobacter ureafaciens, which produces di-d-fructose 2,6':6,2' dianhydride (difructose anhydride iv) from levan by an intramolecular levan fructosyl transfer (ilft) reaction, was found to produce a trioligofructan and a tetraoligofructan from levan in the presence of levanbiose, indicating the intermolecular fructosyl and levanbiosyl transfer (lft and lbt) reactions. the tri- and tetraoligofructans were identified ...19854030745
in situ immunological determination of basic carbohydrate structures of gangliosides on thin-layer immunological method for the determination of the basic carbohydrate structure of gangliosides by using a thin-layer chromatographic immunostaining technique was developed. after high-performance thin-layer chromatography of gangliosides, the chromatogram is treated with a 0.4% polyisobutylmethacrylate solution. arthrobacter ureafaciens neuraminidase is then applied to the separated gangliosides in situ on the chromatographic plate. this procedure will remove both external and internal sialic ...19854037308
an erythromycin-resistance gene from an erythromycin-producing strain of arthrobacter sp.a gene (erma) coding for a presumed erythromycin-resistance (err) determinant from an er-producing arthrobacter sp. strain (nrrlb3381) was isolated from a gene bank in phage vector lambda 2001 by probing with a streptomyces err gene. strongly hybridizing fragments were subcloned and the appropriate segments sequenced. the erma gene is 76 mol% g + c in content and specifies a protein of 340 aa with an mr of 37454. s1 nuclease mapping and primer extension identified the putative promoter, which re ...19854043733
sensitive enzyme-immunostaining and densitometric determination of ganglio-series gangliosides on thin-layer plate: pmol detection of gangliosides in cerebrospinal immunochemical method has been developed for sensitive detection and determination of ganglio-series gangliosides using thin-layer chromatography/enzyme-immunostaining. after chromatography of gangliosides, the plate was treated with arthrobacter ureafaciens sialidase to remove all sialic acids from ganglio-series gangliosides. for the complete hydrolysis of gangliosides, sodium taurodeoxycholate was found to be required. the resulting asialo-glycolipids, ga2 and ga1 were reacted first with a ...19863511969
initial reactions of xanthone biodegradation by an arthrobacter sp.this study examined the catabolism of xanthone by an arthrobacter sp. (strain gfb100) capable of growth on xanthone as its main source of carbon and energy. an early catabolic intermediate was 3,4-dihydroxyxanthone. this compound was isolated from the growth medium of a mutant strain of the arthrobacter sp. which lacked the xanthone-inducible dihydroxyxanthone ring-fission dioxygenase of the wild-type strain. cell extracts from wild-type xanthone-grown cells oxidized 3,4-dihydroxyxanthone to a y ...19863745120
effect of neuraminidase treatment of cells and effect of soluble glycoproteins on type 3 reovirus attachment to murine l cells.the effect of pretreatment of murine l cells with bacterial neuraminidases on type 3 reovirus attachment was examined. we observed that such treatments resulted in a 60 to 80% decrease of subsequent attachment of 35s-labeled type 3 reovirus in a time- and dose-dependent manner. this result was specific for removal of cell surface sialic acid residues since the specific neuraminidase inhibitor 2-deoxy-2,3-dehydro-n-acetyl neuraminic acid completely prevented the observed effect. although the tota ...19854057353
isolation and characterization of influenza c virus inhibitors in rat serum.two inhibitors against haemagglutination by influenza c virus were isolated from pooled sera of normal rats by sequential chromatography on blue sepharose cl 6b, ultrogel aca 2, and deae-cellulose. the two inhibitors were identified as alpha 1-macroglobulin and murinoglobulin by comparison with the authentic samples. these inhibitors abolished the haemagglutination by influenza c virus strains but did not affect the haemagglutination by influenza a and b virus strains. haemagglutination inhibiti ...19854060850
sulindac oxidation/reduction by microbial cultures; microbial models of mammalian metabolism.the oxidation and reduction of the sulphoxide moiety of the anti-inflammatory agent sulindac was investigated to explore microbial systems exhibiting parallels of known mammalian metabolism. of 24 cultures initially screened, four catalysed the expected reactions in analytical studies. arthrobacter species (atcc 19140) and sporobolomyces pararoseus (atcc 11386) produced sulindac sulphide, aspergillus alliaceus (nrrl 315) produced sulindac sulphone, and nocardia corallina (atcc 19070) produced bo ...19854072250
a comparison of the electrophoretic properties of dehydrogenases from arthrobacter, agrobacterium, and rhizobium. 19714106185
[characteristics of the development and biosynthetic activity of a mixed culture of microorganisms].the growth of microbial populations and their biosynthesis of proteinases with the fibrinolytic activity were studied in an artificial ecosystem composed of a strain producing the enzymes (nocardia minima 1) and a zero strain (arthrobacter citreus vkm 654). the microorganisms in the association were shown to interact in terms of amensalism via metabolites if the enzyme-producing strain dominated.19853903442
the effect of microorganisms on phytotoxicity of herbicides. i. complex activity of some pesticides on plants. 19724118851
fine structure of arthrobacter crystallopoietes during long-term starvation of rod and spherical stage cells. 19734119517
biotin deficiency in arthrobactger globiformis: comparative cell ultrastructure and nonreplacement of the vitamin by structurally unrelated compounds.the development of aberrant cell forms of arthrobacter globiformis 425 due to biotin deficiency was followed by electron microscopy of ultrathin sections. upon comparison with normal cell growth, aberrant cells developed in the early logarithmic growth phase. several membrane-bound bodies were embedded in a thick matrix, showing that cell division was impaired. mesosomes and cytoplasmic membranes were still present in the abnormal cell although the normal cell wall was absent. this condition per ...19734120069
gram characteristics and wall ultrastructure of arthrobacter crystallopoietes during coccus-rod morphogenesis.arthrobacter crystallopoietes growing exponentially as cocci were changed to rods by adding succinate to the medium. cells were sampled before, during, and after this transition for gram-staining and ultrastructural studies. cells were gram stained by the standardized method of bartholomew, and all samples were fixed and prepared for thin sectioning in an identical manner. cocci were gram positive, and thin sections demonstrated a gram-positive type of cell wall having an average thickness of 31 ...19734121451
isolation and characterization of a bacteriophage of arthrobacter globiformis.a bacteriophage which reproduces on arthrobacter globiformis atcc 8010 was isolated from soil. this bacteriophage, designated phiag8010, propagates either in soft agar or broth cultures of the host. because of a slow adsorption rate, neither the latent period nor burst size was determined. the mature virion belongs to bradley's group b and exhibits a hexagonal head measuring 69 nm (length) by 60 nm (width) attached to a sheathless tail 120 nm long. the buoyant density of the mature virion is 1.5 ...19734128824
characteristic cytoplasmic structures in microorganisms utilizing n-butane and l-butanol. 19744132610
[degradation of chlorinated benzenes, phenols and cyclohexane derivatives by benzene and phenol utilizing soil bacteria under aerobic conditions (author's transl)]. 19744134769
the negative staining of "difficult" bacteria like arthrobacter globiformis for electron microscopy. 19744134832
characterization of a yeast d-mannan with an alpha-d-glucosyl phosphate residue as an important immunochemical determinant. 19744138972
[isoleucine biosynthesis in mutants for arthrobacter following varying growth conditions]. 19724145585
alternate pathways of l-rhamnose transport in arthrobacter pyridinolis. 19744151601
[taxonomic studies of gram-positive organisms in sewage]. 19744154648
microbial decomposition of organism, which degrades alpha-picoline but also utilizes 2-ethylpyridine or piperidine as alternative growth substrates, has been isolated from soil and characterized as arthrobacter sp. alpha-picoline-grown cells oxidize 2-ethylpyridine and vice versa. other pyridine derivatives tested are neither utilized as growth substrates nor oxidized by the organism. alpha-picolinate and 2-hydroxy-6-methylpyridine are not metabolized, indicating that degradation is neither initiated by methyl oxidatio ...19744157133
redox reactions in hydrocortisone transformation by arthrobacter globiformis cells.hydrocortisone and prednisolone transformation by arthrobacter globiformis cells in aerobic and anaerobic conditions was studied. 3-ketosteroid-1-en-dehydrogenase activity was shown to be the major factor regulating the direction of transformation. when it is high (aerobic conditions), the end products of hydrocortisone transformation are prednisolone or its 20 beta-hydroxy derivative. the latter is produced via 1-en-dehydrogenation, which is not a limiting stage of the process. low 3-ketosteroi ...19854046604
regulation of 3-ketosteroid-1-en-dehydrogenase activity of arthrobacter globiformis cells by a respiratory has been shown that 3-ketosteroid-1-en-dehydrogenase localized in a cytoplasmic membrane donates reducing equivalents to a respiratory chain directly which passes them over to oxygen. microbial hydrocortisone oxidation is coupled with energy generation in the form of the h+ transmembrane potential. electron transfer via a respiratory chain is the limiting stage in the process of hydrocortisone 1-en-dehydrogenation.19854046608
[mechanism of induction of nicotine degrading enzymes in arthrobacter oxydans]. 19694186896
biotin deficiency in arthrobacter globiformis: fine structure and effect of physical stress. 19704192256
degradation of endrin, aldrin, and ddt by soil microorganisms.twenty microbial cultures which had been shown to degrade dieldrin were tested to determine their ability to degrade endrin, aldrin, ddt, gamma isomers of benzenehexachloride (gamma-bhc), and baygon. all isolates were able to degrade ddt and endrin, whereas 13 degraded aldrin. however, none of them was able to degrade baygon or gamma-bhc.19704192889
studies on the antigenic activities of yeasts. v. effect of alpha-mannosidase digestion on the immunochemical properties of the mannan of saccharomyces cerevisiae. 19704194658
extractable lipids of gram-negative marine bacteria: phospholipid composition.phospholipid compositions of 20 strains of marine and estuarine bacteria were determined. results showed that phospholipids of marine bacteria differed very little from those of nonmarine organisms with phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol being the predominant phospholipids in all strains examined. lyso-phosphatidylethanolamine occurred in significant quantities among a number of the marine bacteria, and two of the isolates contained significant quantities ...19734197274
na+-dependent transport of threonine in brevibacterium flavum. 19734198929
immersion refractometry of isolated bacterial cell walls.immersion-refractometric and light-scattering measurements were adapted to determinations of average refractive indices and physical compactness of isolated bacterial cell walls. the structures were immersed in solutions containing various concentrations of polymer molecules that cannot penetrate into wall pores, and then an estimate was made of the polymer concentration or the refractive index of the polymer solution in which light scattering was reduced to zero. because each wall preparation w ...19734201772
the conversion of bisnorbiotin and bisnordethiobiotin to biotin and dethiobiotin, respectively, by microorganisms. 19734204631
effect of copper on the composition of bacterial cell wall peptides. 19734205241
studies on the activated sludge bacteria participating in the biodegradation of methanol, formaldehyde and ethylene glycol. i. isolation and identification. 19744209888
studies on the activated sludge bacteria participating in the biodegradation of methanol, formaldehyde and ethylene glycol. ii. utilization of various carbon and nitrogen compounds. 19744209889
structure of the mannan form saccharomyces strain fh4c, a mutant constitutive for invertase biosynthesis. i. significance of phosphate to the structure and refractoriness of the molecule. 19744211220
biological deactivation of rg seed dressing in soil. 19744214179
a method of "staining" bacterial cells for the scanning electron microscope. 19724115392
microbiological assay of plant growth regulators. 19684172269
a contrast technique for microbiological assay of plant growth regulators. 19684172270
mutations in arthrobacter affecting formation of extracellular protease. 19744215282
[metabolic products of microorganisms. 134. stenothricin, a new inhibitor of the bacterial cell wall synthesis (author's transl)]. 19744215397
[nucleotide composition of the dna of listeria]. 19744218016
[morphological characteristics verus the physiological and biochemical regularities of the activity of microoroganisms during periodic and continuous cultivation (literature review)]. 19744218032
[microbiological transformation of herbicides in co-oxidation conditions]. 19744218859
the phosphofructokinase of arthrobacter crystallopoietes. 19694247776
-glutamyl-glutamic acid, an interpeptide bridge in the murein of some micrococci and arthrobacter sp. 19714256044
a new type of peptide subunit in the murein of arthrobacter strain j39. 19714259817
[amino acid sequence of the murein of sarcina ventriculi and sarcina maxima]. 19724260172
chemical modifications of the postsynaptic naja naja neurotoxins. 19724267235
the stimulation of free radical formation in arthrobacter sp. by manganous ions. 19654285355
the metabolism of coumarin by a microorganism. v. melilotate hydroxylase. 19664285850
influence of clay minerals on microorganisms. i. montmorillonite and kaolinite on bacteria. 19664289932
hydroxamic acids in nature.the hydroxamic acid bond occurs in products from fungi, yeast, bacteria, and plants. the -con(oh)-bond arises by oxidation of a free or bound amino group in a unit structure which is often closely related to conventional amino acids. products are known with one, two, or three hydroxamic acid groups per molecule. the chemistry of the ferrichrome type compounds, which are ferric trihydroxamate-containing peptides, has been worked out in detail and includes a complete crystallographic analysis of t ...19674304945
selective inhibition of bacterial enzymes by free fatty acids.octanoic acid inhibits, in vitro, the bacterial enzymes glucose-6-phosphate dehydrogenase, phosphofructokinase, pyruvate kinase, fumarase, lactate dehydrogenase, and the malic enzyme of arthrobacter crystallopoietes. the free fatty acid appears to act as an inhibitor of lipogenesis, although it does not affect the rate of gluconeogenesis. to demonstrate that this inhibition may be of physiological significance in vivo, those enzymes not involved in lipogenesis, such as fructose-1, 6-diphosphatas ...19694306971
steroid derivatives. lxxi. the microbial dehydrogenation of 17beta-hydroxy-17alpha-methyl-5alpha-androstan-3-one. 19704319876
structures of the naturally occurring hydroxamic acids, fusarinines a and b. 19684320437
phosphatases: applications of new methods for their separation. 19714326442
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