pyrrolidine metabolism and its regulation in arthrobacter sp. 19751221030
peptidoglycan compositions of a new strain of arthrobacter crystallopietes during sphere-rod morphogenesis.arthrobacter crystallopoieties atcc 15481 was used to isolate a new strain. designated arthrobacter crystallopoieties epsr-16, which had a mass doubling time in brain heart infusion broth and in glucose/salts/yeast extract medium of 30 min compared to 2.40 h for the parent strain in similar media. the growth rates for the new strain and for the parent were close to 12 h in glucose/salts medium. the new strain formed well-separated cocci and diplococci in glucose/salts medium, and upon nutrient s ...19751201285
[microbiological transformation and degradation of pesticides]. 19751206144
effects of the membrane action of tetralin on the functional and structural properties of artificial and bacterial membranes.tetralin is toxic to bacterial cells at concentrations below 100 mumol/liter. to assess the inhibitory action of tetralin on bacterial membranes, a membrane model system, consisting of proteoliposomes in which beef heart cytochrome c oxidase was reconstituted as the proton motive force-generating mechanism, and several gram-positive and gram-negative bacteria were studied. because of its hydrophobicity, tetralin partitioned into lipid membranes preferentially (lipid/buffer partition coefficient ...19921314806
the microbial degradation of cyclohexane carboxylic acid. 19751202160
amino acid composition and physiochemical characterization of chondroitinase from arthrobacter aurescens.the amino acid and carbohydrate compositions of chondroitinase ac [ec] from arthrobacter aurescens were determined, and its physicochemical properties were examined. 1. the enzyme has been shown to be a glycoprotein containing mannose, glucose, glucosamine, and glucuronic acid (3:5:4:2). 2. its molecular wieght was estimated to be 76,000 by gel filtration on sephadex g-200, 75,000-80,000 by sds disc electrophoresis, and 75,800 by sedimentation veolcity. no subunits were detected in the m ...19751225917
enzymic hydrolysis of di-d-fructofuranose 1, 2'; 2, 3' dianhydride with arthrobacter ureafaciens.enzymic hydrolysis of di-d-fructofuranose 1, 2'; 2, 3' dianhydride with the bacteria arthrobacter ureafaciens was studied to elucidate its mechanism. hydrolysis of the difructose dianhydride to d-fructose, which did not occur with yeast invertase [ec], was found to occur on incubation with an enzyme preparation from an autolysate of the above bacteria. however, incubation with enzyme which had been treated at 60 degrees for 30 min yielded an intermediate hydrolysis product. the product ...19751225919
cell growth and enzyme synthesis of a mutant of arthrobacter sp. (dsm 3747) used for the production of l-amino acids from d,l-5-monosubstituted hydantoins.a microorganism with the ability to form l-tryptophan from d,l-5-(3-indolyl-methyl)hydantoin (d,l-5-imh) was isolated and identified as arthrobacter sp. (dsm 3747). after isolation of a mutant with high tryptophan production activity but low tryptophan degradation, cultural conditions were optimized to achieve high amounts of biomass with good specific activities concerning the enzymatic hydantoin-cleaving reactions. the ability of the microorganism to perform these bioconversions was found to b ...19901366910
a specific endonuclease from arthrobacter luteus. 19761271462
transformation of microcrystalline hydrocortisone by free and immobilized cells of arthrobacter simplex.the transformation of microcrystalline hydrocortisone by free and immobilized cells of arthrobacter simplex resulted in formation of prednisolone. the effect of medium composition, non-ionogenic surfactants and exogenous electron acceptors on the delta 1-dehydrogenase activity of free and immobilized cells is described.19901366456
production of l-tryptophan from d,l-5-indolylmethylhydantoin by resting cells of a mutant of arthrobacter species (dsm 3747).the reaction parameters and the stereospecificity of the enzymatic cleavage of d,l-5-indolylmethylhydantoin in producing l-tryptophan with resting cells of arthrobacter sp. dsm 3747 were studied. when intact cells were tested, the optimal ph was between 8.5 and 9.0 and the optimal temperature was 50 degrees c. both, l-n-carbamoylase and hydantoinase could be stabilized over 24 h at 30 and 40 degrees c by the addition of d,l-5-indolylmethylhydantoin. furthermore, the hydantoinase was stable over ...19901366911
trimethyl lead degradation by free and immobilized cells of an arthrobacter sp. and by the wood decay fungus phaeolus schweinitzii.the continuing production of leaded petrol generates liquid wastes containing recalcitrant trialkyl lead, for which no suitable chemical treatment has been formulated. this investigation explores the feasibility of using microorganisms to catalyse the rate-limiting step of trimethyl lead degradation to dialkyl lead; this disproportionates chemically to give, ultimately, pb2+ which is treatable by classical methods. an arthrobacter sp. and a wood decay macrofungus, phaeolus schweinitzii provide n ...19901366364
stereospecific microbial reduction of 4,5-dihydro-4-(4-methoxyphenyl)-6-(trifluoromethyl-1h-1)-benzazepin+ ++-2-o ne.a key intermediate, (3r-cis)-1,3,4,5-tetrahydro-3-hydroxy-4-(4-methoxyphenyl)-6-(trifluorome thyl)- 2h-1-benzazepin-2-one (compound ii or sq32191), with high optical purity was made by the stereoselective microbial reduction of the parent ketone 1. several strains of bacterial and yeast cultures were screened for the ability to catalyse the stereoselective reduction of 4,5-dihydro-4-(4-methoxyphenyl)-6-(trifluoromethyl)-1h-1-benzazepin++ +-2,3-dione [compound i or sq32425]. microorganisms from t ...19911368001
immobilization of arthrobacter simplex in thermally reversible hydrogels: effect of gel hydrophobicity on steroid conversion.arthrobacter simplex cells have been immobilized in a series of thermally reversible hydrogels having different gel hydrophobicities. steroid conversion from hydrocortisone to prednisolone via the delta 1-dehydrogenase system was greatly affected by the relative hydrophobicities of the gel matrices, which were prepared by copolymerizing varying ratios of n-isopropylacrylamide to acrylamide. the characteristics of the immobilized cells, such as optimal temperatures, km values, and the effects of ...19911367990
delta'-dehydrogenation of steroids by arthrobacter simplex immobilized in calcium polygalacturonate beads.arthrobacter simplex atcc 6946 (viable cells) was immobilized in a calcium polygalacturonate gel. the trapped cells were used for repeated batchwise bioconversion of steroids. reichstein's compound s and hydrocortisone were dehydrogenated introducing a double bond between c1 and c2 of ring a. the products 1-dehydro s and prednisolone, respectively, were identified by high pressure liquid chromatography. steroid dehydrogenase activity increased in the system when an artificial electron acceptor, ...19911367800
the use of free and immobilised arthrobacter simplex in organic solvent/aqueous two-liquid-phase reactors.the use of free and immobilised arthrobacter simplex (ncib 8929) for steroid delta 1-dehydrogenation in two-liquid-phase, stirred-tank reactors has been compared. product formation is related to the logarithm of the water-octanol partition coefficient (log p) of the organic solvent employed, but the relationship is different for the two forms of the biocatalyst. no reaction was seen with either biocatalyst in media containing solvents of log p less than or equal to 2.5. for free bacteria, produc ...19901366455
continuous delta 1-hydrocortisone dehydrogenation with in situ product recovery.a continuous aerated process for delta 1-hydrocortisone dehydrogenation by polyacrylamide-hydrazide (paah) bead-entrapped a. simplex cells was developed. the process allows for stable conversion of 1.6 g l-1 hydrocortisone (x 5 the solubility in water), made possible by the incorporation of selected cosolvent [5% (v/v) triethyleneglycol]. a large difference in substrate and product solubilities in the cosolvent-buffer medium allowed for in situ product recovery in an aerated, fluidized-bed, immo ...19911367997
sites of cadmium uptake in bacteria used for biosorption.electron microscopy and x-ray spectroscopy were used to determine location and type of cadmium biosorption on and in bacteria, some of which produced extracellular polymers. examined arthrobacter and pseudomonas species appear to have detoxification systems that precipitate cadmium internally irrespective of whether or not they excrete polymers. capsular klebsiella aerogenes strains showed minimal intracellular uptake but over a 5-100 mg dm-3 cd range produced the highest net metal removal level ...19901366533
nadh production from nad+ with a formate dehydrogenase system involving immobilized cells of a methylotrophic arthrobacter strain.a convenient and economical method of nadh production from nad+ has been established using a formate dehydrogenase system involving immobilized cells of a methanol-utilizing bacterium. arthrobacter sp, km62. four kinds of cell entrapment were studied. an immobilized cell preparation showing a high nadh production activity was obtained by entrapment in a kappa-carrageenan gel lattice. the nadh-producing activity of the immobilized cells was investigated under various conditions. the nadh-producin ...19901366576
purification and properties of an arthrobacter oxydans p52 carbamate hydrolase specific for the herbicide phenmedipham and nucleotide sequence of the corresponding gene.arthrobacter oxydans p52 isolated from soil samples was found to degrade the phenylcarbamate herbicides phenmedipham and desmedipham cometabolically by hydrolyzing their central carbamate linkages. the phenylcarbamate hydrolase (phenmedipham hydrolase) responsible for the degradative reaction was purified to homogeneity. the enzyme was shown to be a monomer with a molecular weight of 55,000. a 41-kb wild-type plasmid (php52) was identified in a. oxydans p52, but not in a derivative of this strai ...19921400211
acsi, a new restriction endonuclease from arthrobacter citreus 310 recognizing 5'-pu decreases aattpy-3'. 19921322532
screening of whole-cell immobilization procedures for the delta 1-dehydrogenation of steroids in organic medium.different whole-cell immobilization methods and conditions were tested for the delta 1-dehydrogenation of 6-alpha-methyl-hydrocortisone-21-acetate with arthrobacter simplex cells, in n-decane-1-ol and chloroform. among the entrapment methods, polyurethane foams gave the best productivity of n-decane-1-ol, but could not be used in chloroform. partition and diffusional barriers appeared to be the factors limiting productivity in entrapped-cell systems. the coentrapment of enzyme-stabilizing (glyce ...19921368890
multi-recognition capability of e-selectin in a dynamic flow system, as evidenced by differential effects of sialidases and anti-carbohydrate antibodies on selectin-mediated cell adhesion at low vs. high wall shear stress: a preliminary note.e-selectin has a "multi-recognition" capability in terms of epitope binding specificity, depending on adhesion conditions (static vs. low- or high-shear stress dynamic systems). specifically, (i) adhesion based on expression of alpha 2-->3 sialylated le(x) (sle(x)) is prominent under static or low shear stress dynamic conditions; (ii) adhesion under high shear stress dynamic conditions does not depend on the known sle(x) species, but rather on lex with an adjacent unidentified sialosyl substitut ...19921282810
structural study of cell wall mannan of a candida albicans (serotype a) strain.the structure of the mannan of candida albicans nih a-207 strain (serotype a) was investigated by adopting mild acetolysis followed by enzymolysis with an arthrobacter gjm-1 exo-alpha-mannosidase. the resultant oligosaccharides, from pentaose to octaose (where manp = d-mannopyranose), were identified as manp beta (1----2)manp alpha (1----2)manp alpha (1----2)manp alpha (1----2)manp, manp beta (1----2)manp beta (1----2)manp alpha (1----2)manp alpha (1----2)- manp alpha (1----2)manp, manp beta (1- ...19921368047
effects of temperature on the growth of psychrophilic bacteria from glaciers.growth of five strains of psychrophilic bacteria (four arthrobacter and one pseudomonas) isolated from glacial deposits was studied at different temperatures. three strains were facultative psychrophiles, having an optimum temperature for growth at about 25-28 degrees c and a maximum at about 32-34 degrees c. the two arthrobacter glacialis strains were found to be obligate psychrophiles with an optimum at 13-15 degrees c and a maximum at 18 degrees c. arrhenius plots showed that a. glacialis cou ...19761277005
[cloning and expression of the arthrobacter globiformis fcb genes in bacillus subtilis].the fcb genes of arthrobacter globiformis kzt1 coding for the dehalogenase (4-chlorobenzoate-4-hydroxylase) activity have been cloned. the characteristics of fcb genes expression have been studied. the recombinant strains of bacillus subtilis 6jm15 (pcbs 311) and 6jm15 (pcbs1) have shown the decreased level of substrate dehalogenation as compared with the one in the parent strain kzt1 and the recombinant strains of escherichia coli and pseudomonas putida.19921301499
colonization of soil by arthrobacter and pseudomonas under varying conditions of water and nutrient availability as studied by plate counts and transmission electron microscopy.arthrobacter globiformis and a pseudomonas soil isolate were incubated separately and in combination in soil that had been presterilized by autoclaving. growth and other responses of the cells in situ in this soil were monitored by plate counts and transmission electron microscopy examinations of cell sections. during the soil incubations, some of the samples were first allowed to dry and then were remoistened with water or with a dilute or a concentrated nutrient solution. based on plate counts ...19761267449
purification and some properties of beta-fructofuranosidase i from arthrobacter sp. k-1.arthrobacter sp. k-1, isolated from soil, produces beta-fructofuranosidase. this enzyme preparation was separated into three fractions (i, ii, and iii) by butyl-toyopearl 650 m column chromatography. the beta-fructofuranosidase i was purified to homogeneity by disc-electrophoresis after consecutive column chromatographies. the enzyme had a molecular weight of 52,000 by sds-polyacrylamide gel electrophoresis and 51,000 by gel filtration with ultrogel aca 44, and an isoelectric point of 4.3. the e ...19901368550
evidence for two distinct phosphonate-degrading enzymes (c-p lyases) in arthrobacter sp. glp-1.arthrobacter sp. glp-1 can utilize a wide range of organophosphonates as its sole source of phosphorus. the in-situ formation of sarcosine and methane from glyphosate and methanephosphonic acid respectively was studied. these two processes are differentially induced during phosphorus-deprivation. methanephosphonic acid strongly inhibits glyphosate degradation (i50 10 microm), but glyphosate has very little effect on methane generation (i50 150 mm). the pattern of inhibition by other organophosph ...19911368477
action of levan fructotransferase of arthrobacter ureafaciens on three oligosaccharides containing a bifurcose residue. 19901368538
microbial degradation of propoxur in turfgrass soil.this study was conducted to determine the degradation rates in turfgrass soil over a 12-month period after a single field application of propoxur and to isolate microorganisms from the soil capable of degrading the insecticide. soil samples were collected from a turfgrass experimental site near fort lauderdale, fl one week before the field application of propoxur, and over a 12-month period after the field application. mineralization rates in surface (0-15 cm depth) and subsurface (15-30 cm dept ...19921401729
cloning and nucleotide sequence of the csp1 gene encoding ps1, one of the two major secreted proteins of corynebacterium glutamicum: the deduced n-terminal region of ps1 is similar to the mycobacterium antigen 85 complex.two proteins, ps1 and ps2, were detected in the culture medium of corynebacterium glutamicum and are the major proteins secreted by this bacterium. no enzymatic activity was identified for either of the two proteins. immunologically cross-reacting proteins were found in a variety of c. glutamicum strains but not in the coryneform arthrobacter aureus. the gene encoding ps1, csp1, was cloned in lambda gt11 using polyclonal antibodies raised against ps1 to screen for producing clones. the csp1 gene ...19921406274
action of levan fructotransferase of arthrobacter ureafaciens on a mixture of branched levanpentasaccharides. 19921368345
whipple's disease, familial mediterranean fever, adult-onset still's disease, and enteropathic arthritis.whipple's disease is a rare multisystem disorder of infectious etiology. efforts to culture the responsible organism have been unsuccessful. nucleotide sequencing and amplification of bacterial 16s ribosomal dna revealed the organism to be most similar to bacteria of the rhodococcus, streptomyces, and arthrobacter genera. several clinical studies of the long-term use of colchicine for the treatment of familial mediterranean fever demonstrate its utility for symptom control and prevention of comp ...19921380277
inhibition of development of kaposi's sarcoma-related lesions by a bacterial cell wall vitro and in vivo model systems for the study of human immunodeficiency virus (hiv)-associated kaposi's sarcoma (ks) were used to evaluate compounds for their potential as therapeutic agents. a sulfated polysaccharide-peptidoglycan compound (sp-pg) produced by bacteria controlled the in vitro growth of acquired immunodeficiency syndrome (aids)-associated, ks-derived spindle-shaped cells (aids-ks cells) at noncytotoxic concentrations. angiogenesis induced by aids-ks cells in the chicken chorio ...19921371891
epimerization of reducing terminal groups of (1----2)-linked d-gluco- and d-manno-disaccharides in aqueous sodium hydroxide. 19921394294
anomeric specificity of d-xylose isomerase.crystal structures of complexes of d-xylose isomerase with deoxysugars have been determined. deoxynojirimycin is a structural analogue of alpha-pyranose and mimics the binding of these aldose substrates. the structure of this complex supports the hypothesis that an imidazole group catalyzes ring opening of the pyranose. the steric restrictions in the active site of the enzyme prevent a beta-pyranose from binding in the same way. for the reverse reaction with ketoses, the anomeric specificity is ...19921457418
structure of the d-mannan of the pathogenic yeast, candida stellatoidea atcc 20408 (type ii) strain, in comparison with that of c. stellatoidea atcc 36232 (type i) strain.acid treatment of the cell-wall d-mannas of candida stellatoidea strains atcc 36232 (type i, a3 strain) and atcc 20408 (type ii, a2 strain) gave (1----2)-linked beta-d-manno-oligosaccharides (dp 2-5), whereas treatment with alkali gave the (1----2)-linked alpha-d-mannobiose. conventional acetolysis of the acid- and alkali-treated d-mannan of the a3 strain gave oligosaccharides consisting of (1----2)- and (1----3)-linked alpha-d-mannopyranose residues, similar to those of candida albicans serotyp ...19921394307
structure of the n- and o-glycans of the a-chain of human plasma alpha 2hs-glycoprotein as deduced from the chemical compositions of the derivatives prepared by stepwise degradation with exoglycosidases.the structure of the glycans of the a-chain of human plasma alpha 2hs-glycoprotein was established from the chemical compositions of its derivatives prepared by sequential enzymatic degradation of the carbohydrate moiety, from the determination of the kind and amount of the monosaccharides liberated after each step of the enzymatic digestion, and from the distinct specificity of the highly purified exoglycosidases. the exoglycosidases were three sialidases (vibrio cholerae, fowl plague virus, an ...19921457416
crystal structure analysis, refinement and enzymatic reaction mechanism of n-carbamoylsarcosine amidohydrolase from arthrobacter sp. at 2.0 a resolution.n-carbamoylsarcosine amidohydrolase from arthrobacter sp., a tetramer of polypeptides with 264 amino acid residues each, has been crystallized and its structure solved and refined at 2.0 a resolution, to a crystallographic r-factor of 18.6%. the crystals employed in the analysis contain one tetramer of 116,000 m(r) in the asymmetric unit. the structure determination proceeded by multiple isomorphous replacement, followed by solvent-flattening and density averaging about the local diads within th ...19921381445
mineralization of 2-chloro- and 2,5-dichlorobiphenyl by pseudomonas sp. strain ucr2.pseudomonas sp. strain ucr2 was isolated from a multi-chemostat mating experiment between a chlorobenzoate-degrader, pseudomonas aeruginosa strain jb2, and a chlorobiphenyl-degrader, arthrobacter sp. strain b1barc. strain ucr2 differed from either of the parental organisms in that it grew on both 2-chloro- and 2,5-dichlorobiphenyl with concomitant release of chloride. phenotypic typing by the biolog system indicated that strain ucr2 shared greater similarity with strain jb2 (88%) than strain b1b ...19921459405
cloning and sequence analysis of genes for dehalogenation of 4-chlorobenzoate from arthrobacter sp. strain su.strains of arthrobacter catalyze a hydrolytic dehalogenation of 4-chlorobenzoate (4-cba) to p-hydroxybenzoate. the reaction requires atp and coenzyme a (coa), indicating activation of the substrate via a thioester, like that reported for pseudomonas sp. strain cbs3 (j. d. scholten, k.-h. chang, p. c. babbit, h. charest, m. sylvestre, and d. dunaway-mariano, science 253:182-185, 1991). the dehalogenase genes of arthrobacter sp. strain su were cloned and expressed in escherichia coli. analyses of ...19921476446
siderophores and related outer membrane proteins produced by pseudomonads isolated from eels and freshwater.a total of 46 environmental pseudomonads, together with six type strains, were examined for their siderophore-producing activity. all strains were able to grow under iron-limiting conditions, gave orange halos in the cas agar assay, and produced hydroxamates, and some of them also produced phenolate-type compounds. bioassays showed that all strains, except pseudomonas aeruginosa, promoted growth of mutant strain arthrobacter flavescens jg-9, deficient in hydroxamate production, and some of them ...19921459416
stability of arthrobacter d-xylose isomerase to denaturants and heat.there was no inactivation of mg(2+)-containing arthrobacter d-xylose isomerase up to 1 h in 0-8 m-urea at 22 degrees c, but over this range there was rapid reversible dissociation into fully active dimers with a midpoint around 4 m-urea, as shown by gradient urea gels with an activity stain, and by ion-exchange chromatography and gel filtration in urea buffers. these dimers must have the a-b* conformation, since the tetramer could dissociate into a-a*, a-b or a-b* dimer conformations, but only r ...19921497626
biosynthesis of a nonphysiological sialic acid in different rat organs, using n-propanoyl-d-hexosamines as this study it could be shown that in rat the normally occurring n-acetyl neuraminic acid can be modified in its n-acyl moiety by in vivo administration of the chemically synthesized n-propanoyl precursors, n-propanoyl-d-glucosamine or n-propanoyl-d-mannosamine. it could be shown that each of these nonphysiological amino sugar analogues was incorporated into both membrane and serum glycoproteins. after treatment of rats with radiolabeled n-[acyl-1-14c]d-mannosamine, radioactivity could be remo ...19921512235
chemotaxonomic differentiation of coryneform bacteria isolated from biofilters.coryneform bacteria that were isolated from biofilters which are used for waste gas treatment of animal-rendering plant emissions were differentiated and partially identified by using chemotaxonomic methods. on the basis of the results of a numerical analysis of whole-cell fatty acid profiles, 79 isolates were divided into two major groups; the members of the first group contained saturated and monounsaturated fatty acids, whereas the members of the second group were characterized by iso- and an ...19921503976
[biotransformation of zearalenone].the conversion of zearalenone by some strains of microorganisms was investigated. when the selective strains of rhodotorula sp., arthrobacter sp., saccharomyces sp., and candida sp. were incubated by shaking or standing at 28 degrees c for 72 h with an alcoholic solution of zearalenone as the substrate at a concentration of 2-10 mg/ml ethanol (50-100 micrograms/ml medium), it was readily converted to give zearalenols, consisting either mainly of the alpha-isomer (e.g. 96% in case of rhodotorula ...19921413733
application of a microbial sensor for determination of short-chain fatty acids in raw milk samples.a microbial sensor system, based on the use of immobilized arthrobacter nicotiana and an oxygen electrode, was applied to determine free short-chain fatty acids in raw milk samples and the result was compared with gas chromatography (gc) and a titrimetric method. the sensor response was linearly related to the concentration of short-chain fatty acids obtained by gc (n = 10, r = 0.92) and to the total concentration of free fatty acids obtained by titrimetric measurement (n = 10, r = 0.78). this r ...19921502853
biosynthesis and distribution of n-carboxyalkyl amino acids (opines) in bacteria. 19921410788
[microbiological production of 3-oxo-bisnorchola-1, 4-dien-22-oic acid from cholesterol by an arthrobacter 82].among nineteen strains of arthrobacter which showed to be able to decompose cholesterol in preliminary experiments, a strain of arthrobacter 82 was selected for microbiological production of 3-oxo-bisnorchola-1,4-dien-22-oic-acid (bnc) from cholesterol. the yield is over of 50% weight percent concentration of 0.25% in the presence of cobalt sulfate. the main intermediate in such a conversion process is cholestenone. lower glucose and higher corn steep liquor concentration were favorable for side ...19921413734
purification and characterization of an nad(+)-linked formaldehyde dehydrogenase from the facultative rump cycle methylotroph arthrobacter p1.when arthrobacter p1 is grown on choline, betaine, dimethylglycine or sarcosine, an nad(+)-dependent formaldehyde dehydrogenase is induced. this formaldehyde dehydrogenase has been purified using ammonium sulphate fractionation, anion exchange- and hydrophobic interaction chromatography. the molecular mass of the native enzyme was 115 kda +/- 10 kda. gel electrophoresis in the presence of sodium dodecyl sulphate indicated that the molecular mass of the subunit was 56 kda +/- 3 kda, which is cons ...19921416916
the structural genes encoding co dehydrogenase subunits (cox l, m and s) in pseudomonas carboxydovorans om5 reside on plasmid phcg3 and are, with the exception of streptomyces thermoautotrophicus, conserved in carboxydotrophic bacteria.employing deoxyoligonucleotide probes and southern hybridizations, we have examined in carboxydotrophic bacteria the localization on the genome of genes encoding the large, medium and small subunits of co dehydrogenase (coxl, m and s, respectively). in pseudomonas carboxydovorans om5 coxl, m and s were identified on the plasmid phcg3; they were absent on the chromosome. this was evident from positive hybridizations with plasmid dna of the wild-type strain om5 and the absence of hybridizations wi ...19921510563
immunological demonstration of a unique 3,4-dihydroxyphenylacetate 2,3-dioxygenase in soil arthrobacter strains.many bacteria biosynthesize 3,4-dihydroxyphenylacetate 2,3-dioxygenases for growth on aromatic acids, but gram-negative organisms have been most extensively studied. a gram-positive strain containing 2,3-dioxygenase activity was identified as arthrobacter strain mn-1. the 2,3-dioxygenase from strain mn-1 was purified to homogeneity by fast protein liquid chromatography with a mono q anion-exchange column. rabbit polyclonal antidioxygenase antibodies were prepared. ouchterlony double-diffusion an ...19921444392
isolation and characterization of a fluorene-degrading bacterium: identification of ring oxidation and ring fission arthrobacter sp. strain, f101, able to use fluorene as the sole source of carbon and energy, was isolated from sludge from an oil refinery wastewater treatment plant. during growth in the presence of fluorene, four major metabolites were detected and isolated by thin-layer chromatography and high-performance liquid chromatography. 9-fluorenol, 9h-fluoren-9-one, and 3,4-dihydrocoumarin were identified by uv spectra, mass spectrometry, and 300-mhz proton nuclear magnetic resonance. the fourth m ...19921444405
myceloid cell formation in arthrobacter globiformis during osmotic stress.arthrobacter globiformis was grown in a semi-defined liquid medium containing added solutes to determine the effects of osmotic stress on its reproduction and cell morphology. there was a progressive reduction in the specific growth rate during exponential phase as the concentration of nacl was increased, although the final yields of the cultures during stationary phase were not affected. clusters of branching myceloid cells rather than the typical bacillary forms predominated during exponential ...19921644706
metabolic blocks in the degradation of beta-sitosterol by a plasmid-cured strain of arthrobacter oxydans.plasmid-harbouring, sterol-decomposing organism arthrobacter oxydans 317 was treated with sodium dodecylsulphate to obtain a plasmid-cured strain a. oxydans 317 a1 incapable of utilizing 4-androstene-3,17-dione (ad). the strain 317 a1 was unable to degrade beta-sitosterol side chain completely to form ad but could carry out partial degradation as shown by the accumulation of 3-oxochol-4-en-24-oic acid as a major metabolite and 27-norcholest-4-en-3,24-dione as a minor metabolite. the strain could ...19921512707
levels of trehalose and glycogen in arthrobacter globiformis under conditions of nutrient starvation and osmotic stress.cells of arthrobacter globiformis grown in carbohydrate-rich media were found to contain large quantities of low-mr carbohydrates (800 micrograms/mg protein) and only small amounts of amino acids, in addition to high amounts of glycogen (2 mg/mg protein). at increasing osmotic values of the medium, low-mr carbohydrate levels increased to 1300 micrograms/mg protein. low-mr pools were extracted from the cells with hot 75% ethanol, and subjected to thin layer, gel and gas-liquid chromatography. the ...19921575469
hemicellulose bioconversion to polyanionic heteropolysaccharides.anionic polysaccharides, traditionally obtained from plant or algal sources, have a variety of commercial uses. such gums from microorganisms have received increased recent interest. we have initiated a program to investigate the bioconversion of pentosans to rheologically useful anionic extracellular polysaccharides (aeps). a number of earlier-described species, including cryptococcus laurentii, klebsiella pneumoniae, arthrobacter viscosus, and pseudomonas atcc 31260, appear to have potential i ...19921622201
mechanism of d-fructose isomerization by arthrobacter d-xylose isomerase.the mechanism of d-fructose isomerization by arthrobacter d-xylose isomerase suggested from x-ray-crystallographic studies was tested by detailed kinetic analysis of the enzyme with various metal ions at different ph values and temperatures. at d-fructose concentrations used in commercial processes mg2+ is the best activator with an apparent dissociation constant of 63 microm; co2+ and mn2+ bind more strongly (apparent kd 20 microm and 10 microm respectively) but give less activity (45% and 8% r ...19921567370
bacteria that degrade p-chlorophenol isolated from a continuous culture system.two gram-positive coryneform bacteria that degraded p-chlorophenol isolated from a continuous culture system are characterized. isolate b (probably and arthrobacter sp.) completely removed the p-chlorophenol from a medium with a concomitant increase in cell density within 16 h. isolate f similarly removed the p-chlorophenol within 28 h but without an increase in cell density. isolates b and f also removed the p-chlorophenol from a medium with p-chlorophenol as the sole carbon source within 32 an ...19921581863
occurrence of protein phosphorylation in various bacterial species.1. the occurrence of protein phosphorylation in escherichia coli b, bacillus megaterium, bacillus sphaericus, pseudomonas fluorescens and arthrobacter s1-55, was investigated by means of both in vivo and in vitro experiments. 2. in each bacterial species the presence of several phosphorylated proteins was evidenced by gel electrophoresis and autoradiography after either labelling of growing cells with [32p]orthophosphate or incubating cellular extracts with radioactive atp. 3. the analysis of th ...19901693344
effect of bacterial extracellular polymers on the saturated hydraulic conductivity of sand columns.columns were packed with clean quartz sand, sterilized, and inoculated with different strains of bacteria, which multiplied within the sand at the expense of a continuous supply of fresh nutrient medium. the saturated hydraulic conductivity (hcsat) of the sand was monitored over time. among the four bacterial strains tested, one formed a capsule, one produced slime layers, and two did not produce any detectable exopolymers. the last two strains were nonmucoid variants of the first two. only one ...19921622240
reclassification of two strains of arthrobacter oxydans and proposal of arthrobacter nicotinovorans sp. nov.arthrobacter oxydans dsm 419 and dsm 420 have chemical and microbiological properties that are consistent with assignment to the genus arthrobacter. both organisms have the lysine-alanine-threonine-alanine peptidoglycan type. dna-dna pairing studies indicated that a. oxydans dsm 419 should be reclassified as arthrobacter ureafaciens and that a. oxydans dsm 420t forms the nucleus of a distinct genomic species. we propose that a. oxydans dsm 420 should be reclassified as arthrobacter nicotinovoran ...19921581183
molecular mechanics simulations of a conformational rearrangement of d-xylose in the active site of d-xylose isomerase.a proposed reaction mechanism for the enzyme d-xylose isomerase involves the ring opening of the cyclic substrate with a subsequent conformational rearrangement to an extended open-chain form. restrained energy minimization was used to simulate the rearrangement. in the ring-opening step, the substrate energy function was gradually altered from a cyclic to an open-chain form, with energy minimization after each change. the protein/sugar contact energy did not increase significantly during the pr ...19921620692
taxonomic study of the genus brachybacterium: brachybacterium nesterenkovii sp. nov.a new species, brachybacterium nesterenkovii, is proposed for a group of coryneform bacteria that were isolated from milk products. these organisms have morphological, biochemical, and chemotaxonomic characteristics that are peculiar to the genus brachybacterium. in contrast to strains of the only previously described species of the genus, brachybacterium faecium, the representatives of the new species lack glycine in their peptidoglycan, although the peptidoglycan is of the same general type, a ...19921736971
l-lysine production by s-2-aminoethyl-l-cysteine-resistant mutants of arthrobacter globiformis.using mutagenesis with n-methyl-n'-nitro-n-nitrosoguanidine, a number of homoserine auxotrophs have been isolated from a glutamate-producing arthrobacter globiformis excreting l-lysine in good amounts. for further improvement, mutants resistant to the lysine analog s-(2-aminoethyl)-l-cysteine have been isolated from homoserine auxotrophs. for the three potent mutants tested, white's medium was found to be the best. glucose, ammonium nitrate and biotin were found to be optimum at 280 mmol/l, 40 m ...19911841850
[plasmids for biodegradation of 2,6-dimethylpyridine, 2,4-dimethylpyridine, and pyridine in strains of arthrobacter].arthrobacter crysallopoietes strain km-4 degrading 2,6-dimethylpyridine and strain km-4a degrading both 2,6-dimethylpyridine and pyridine, arthrobacter sp. km-4b degrading 2,4-dimethylpyridine were isolated from soil. arthrobacter crystallopoietes km-4 and arthrobacter sp. km-4b contain 100 md plasmids pbs320 and pbs323. arthrobacter crystallopietes km-4a harbours a 100 md and 80 md plasmids. plasmid curing and conjugation transfer results confirm that these plasmids are involved in degradation ...19921454076
isolation method for lysine-excreting mutants of arthrobacter improve the yield of lysine by the isolate, auxotrophic mutants were isolated. among the mutants, only one auxotrophic mutant required vitamin b12. this mutant produced alpha-alanine. about 200 mutants resistant to the lysine analog s-(2-aminoethyl)-l-cysteine were isolated and some of them produced well above the wild type.19911841851
l-phenylalanine production by double auxotrophic mutants of arthrobacter globiformis.a number of tryptophan-plus-tyrosine double auxotrophs have been isolated from a glutamate producing arthrobacter globiformis excreting l-phenylalanine by two-step mutagenesis with n-methyl-n'-nitro-n-nitrosoguanidine. for the three potent mutants tested the medium of alföldi was found to be the best. the optimum tryptophan, tyrosine and biotin concentrations for phenylalanine production of these mutants were 0.5 mmol/l, 0.1 mmol/l and 5 micrograms/l, respectively. at these levels strain tt-39 y ...19911841857
apoi, a unique restriction endonuclease from arthrobacter protophormiae which recognizes 5' raatty 3'. 19921614876
structural organization of the corynebacterium glutamicum plasmid pcg100.pcg100, a 3 kb cryptic plasmid of corynebacterium glutamicum atcc 13058, probably identical with psr1 from c. glutamicum atcc 19223, was characterized. the minimum region for autonomous replication was shown to be contained on a 1.9 kb bglii-ncoi fragment; a 380 bp hindiii-sphi fragment can replicate in the presence of the parental plasmid, which presumably provides a trans-acting replication factor. derivatives of pcg100 are able to replicate in several corynebacterium, brevibacterium and arthr ...19911748866
[effect of normal and specific immune sera on neuraminidase activity].we have got evidence that there is no antigenic relationship reflecting the structural similarity between neuraminidases synthesized by noncholera vibrios and arthrobacter nicotianae. the cross-reactions between the enzymes and heterological antisera were not observed. antibodies against the a. nicotianae neuraminidase inhibited the activity of the enzyme for a glycomacropeptide of milk whey and for components of the blood serum, and had no effect no the neuraminidase from noncholera vibrios. an ...19921528820
interaction of the regulatory protein nicr1 with the promoter region of the pao1-encoded 6-hydroxy-d-nicotine oxidase gene of arthrobacter oxidans.the d,l-nicotine catabolism of the gram-positive soil bacterium arthrobacter oxidans is linked to the presence within the cells of the 160 kb catabolic plasmid pao1. pao1-cured cells lost the catabolic enzymes and reintroduction of pao1 by electroporation into cured cells reestablished the nic+ phenotype. dna band shift assays with extracts from cured and pao1+ cells suggested that pao1 encodes the regulatory protein nicr1. footprint analysis revealed that two homologous palindromes (ir1 and ir2 ...19921630318
mechanism of gram variability in select bacteria.gram stains were performed on strains of actinomyces bovis, actinomyces viscosus, arthrobacter globiformis, bacillus brevis, butyrivibrio fibrisolvens, clostridium tetani, clostridium thermosaccharolyticum, corynebacterium parvum, mycobacterium phlei, and propionibacterium acnes, using a modified gram regimen that allowed the staining process to be observed by electron microscopy (j. a. davies, g. k. anderson, t. j. beveridge, and h. c. clark, j. bacteriol. 156:837-845, 1983). furthermore, since ...19901689718
determination of glycosylation sites using a protein sequencer and deglycosylation of native yeast invertase by endo-beta-n-acetylglucosaminidase.endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae was tested for its capacity to release n-linked sugar chains from native yeast invertase. the enzyme liberated about 80% of the sugar chains from the native invertase. deglycosylated invertase was digested by chymotrypsin or pepsin, and twelve n-acetylglucosamine-containing glycopeptides were isolated. the amino acid sequences of these glycopeptides were analyzed by a protein sequencer, and the elution position of 4-l-aspartylglyc ...19911805802
enzymatic synthesis of novel oligosaccharides by use of transglycosylation activity of endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae.the transglycosylation activity of endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae was used for the enzymatic synthesis of novel oligosaccharides. when (man)6(glcnac)2asn was used as a substrate for the transglycosylation, (man)6glcnac-glc, (man)6glcnac-man, (man)6glcnac-chitobiose, and (man)6glcnac-gentiobiose were synthesized. their structures were identified by hplc, ion spray mass spectrometry, and digestion with glycosidases. endo-beta-n-acetylglucosaminidases hydrolyzed t ...19911804102
3,4-dihydroxyxanthone dioxygenase from arthrobacter sp. strain gfb100.bacterial extradiol ring-fission dioxygenases play a critical role in the transformation of multiring aromatic compounds to more readily biodegradable aromatic or aliphatic intermediates. arthrobacter sp. strain gfb100 utilizes an extradiol meta-fission dioxygenase, 3,4-dihydroxyxanthone dioxygenase (dhxd), in the catabolism of the three-ring oxygen heterocyclic compound xanthone. in this paper, we show that dhxd is a cytosolic enzyme, induced by growth on xanthone and maximally expressed during ...19911768091
the combination of a bacterial polysaccharide and tamoxifen inhibits angiogenesis and tumour growth. 19911713945
riboflavin-dependent expression of flavoenzymes of the nicotine regulon of arthrobacter cells of an arthrobacter oxidans riboflavin-dependent mutant the specific activity of the dl-nicotine-inducible nicregulon enzymes nicotine dehydrogenase (ndh, ec, 6-hydroxy-l-nicotine oxidase (6-hlno, ec and 6-hydroxy-d-nicotine oxidase (6-hdno, ec was shown to be dependent on the supply of the vitamin in the growth medium. experiments designed to identify at which level riboflavin directs the biosynthesis of these flavoenzymes revealed that the steady-state level ...19901700696
phylogeny of the whipple's-disease-associated bacterium.efforts to culture and identify the intracellular bacteria associated with whipple's disease have been unsuccessful. nucleotide sequencing and amplification by the polymerase chain reaction was done on the bacterial 16 s ribosomal dna present in a small-bowel biopsy specimen taken from a patient with whipple's disease. a search by computer for similar rrna sequences filed in databases showed the whipple's-associated organism to be most similar to bacteria of the rhodococcus, streptomyces, and ar ...19911714530
the breast tumor-associated epitope defined by monoclonal antibody 3e1.2 is an o-linked mucin carbohydrate containing n-glycolylneuraminic acid.the breast cancer-associated epitope (mammary serum antigen or msa) defined by monoclonal antibody (mab) 3e1.2 is a neuraminidase-sensitive carbohydrate expressed on muc-1-encoded molecules. however, the reactivity of mab 3e1.2 is also reduced by protease treatment of the mucin, which suggests that 3e1.2 binds to multimers of the sialylated carbohydrate in a protein conformation-dependent manner. the common n-acetyl derivative of neuraminic acid (5-acetylneuraminic acid) is not involved in the e ...19911718585
copper in biological systems. a report from the 6th manziana conference, september 23-27, 1990.enzymes and proteins: ao, amine oxidase; and as proposed in reference 3, bsao, bovine serum ao; ssao, swine serum ao; skdao, swine kidney ao; psao, pea seedling ao; apao, arthrobacter p1ao; madh, methylamine dehydrogenase; aao, ascorbic acid oxidase; alpha-ae, alpha-amidating enzyme; az, azurin; cox, cytochrome c oxidase; cp, ceruloplasmin; dbh, dopamine beta-hydroxylase; go, galactose oxidase; hc, hemocyanin; mt, metallotheonein; nir, nitrite reductase; sod, superoxide dismutase. cofactors: dop ...19911757786
chlorinated biphenyl mineralization by individual populations and consortia of freshwater bacteria.comparative studies were performed to investigate the contribution of microbial consortia, individual microbial populations, and specific plasmids to chlorinated biphenyl biodegradation among microbial communities from a polychlorinated biphenyl-contaminated freshwater environment. a bacterial consortium, designated lps10, was shown to mineralize 4-chlorobiphenyl (4cb) and dehalogenate 4,4'-dichlorobiphenyl. the lps10 consortium involved three isolates: pseudomonas testosteroni (lps10a), which m ...19902117875
indole-3-acetic acid (iaa) production by arthrobacter species isolated from azolla.arthrobacter species, isolated from the leaf cavities and the microsporocarps of the aquatic fern species azolla pinnata and azolla filiculoides, produced indole-3-acetic acid (iaa) in culture when the precursor tryptophan was added to the medium. no iaa production was detected in the absence of tryptophan. maximum iaa formation was obtained in the first 2 d of incubation. part of the tryptophan was transformed to n alpha-acetyl-l-tryptophan.19921564446
substrate interactions of benzene, toluene, and para-xylene during microbial degradation by pure cultures and mixed culture aquifer slurries.benzene, toluene, and p-xylene (btx) were degraded by indigenous mixed cultures in sandy aquifer material and by two pure cultures isolated from the same site. although btx compounds have a similar chemical structure, the fate of individual btx compounds differed when the compounds were fed to each pure culture and mixed culture aquifer slurries. the identification of substrate interactions aided the understanding of this behavior. beneficial substrate interactions included enhanced degradation ...19911746958
transglycosylation activity of endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae.endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae has transglycosylation activity. treatment of (man)6(glcnac)2asn with the enzyme in the presence of free n-acetylglucosamine gave a mixture of (man)6glcnac and (man)6glcnac beta 1----4glcnac mixture. n-acetylglucosamine at the reducing end of the latter sugar chain was found by hplc of the carbohydrate composition and of an exoglycosidase digest of the pyridylamino derivative of the reducing-end residue, and by 400 mhz 1h-nmr spec ...19911776953
occurrence and ultrastructural characterization of bacteria in association with and isolated from azolla caroliniana.the occurrence and ultrastructure of bacteria in leaf cavities of symbiotic azolla caroliniana were examined by transmission electron microscopy. bacteria were observed in all leaf cavities of azolla cultures. five ultrastructurally distinct types of bacteria were observed in each individual leaf cavity. features used to characterize the bacteria included morphology, cell wall structure, and cytoplasmic organization. at least one gram-positive and as many as four gram-negative types of bacteria ...19911785935
experimental apparatus for selection of adherent microorganisms under stringent growth conditions.a bioreactor apparatus is described for studying bacterial attachment. a cyclic, on-off, flow regime was imposed within the apparatus. model calculations illustrate the utility of this flow pattern in the selection and maintenance of slow-growing, adherent organisms. the apparatus is believed to have general utility in testing bacterial attachment influenced by many types of experimental or environmental constraints, including variations in fluid dynamics, presence of toxic substances (metals or ...19911892388
genetic control of degradation of chlorinated benzoic acids in arthrobacter globiformis, corynebacterium sepedonicum and pseudomonas cepacia strains.the strains of arthrobacter globiformis kzt1, corynebacterium sepedonicum kz4 and pseudomonas cepacia kz2 capable of early dehalogenation and complete oxidation of 4-chloro-, 2,4-dichloro-and 2-chlorobenzoic acids, respectively, have been analyzed for the origin of the genetic control of degradation. the occurrence and molecular sizes of plasmids in all the strains have been established. plasmid pbs1501 was shown to control 4-chlorobenzoate dehalogenation in the case of kzt1 strain. the same pos ...19911884993
[cloning of the arthrobacter globiformis fcba gene for dehalogenase and construction of a hybrid pathway of 4-chlorobenzoic acid degradation in pseudomonas putida].the artrobacter globiformis kzt1 fcba gene responsible for dehalogenase (4-chlorobenzoate-4-hydroxylase) activity was cloned in escherichia coli and pseudomonas putida cells. the character of the fcba gene expression was studied. notwithstanding amplification of the gene dose and control of the inducible plac promoter, the level of substrate dehalogenation by recombinant e. coli strains was lower, as compared with that in the original kzt1 strain. cloning of the fcba gene in p. putida kz6r cells ...19911879677
antagonistic effect of coryneform bacteria from red smear cheese against listeria species.a total of 187 coryneform bacteria were isolated from red smear and screened for inhibitory effects against 16 strains of listeria species. culture filtrates from brevibacterium linens (16 strains), arthrobacter nicotianae (4 strains) and arthrobacter nucleogenes (3 strains) showed clear zones of inhibition. the antagonistic effect was seen against 26 to 87% of 91 listeria strains tested. a. nicotianae and a. nucleogenes were more effective against listeria innocua and listeria ivanovii than aga ...19911909545
cloning and expression of the arthrobacter globiformis kzt1 fcba gene encoding dehalogenase (4-chlorobenzoate-4-hydroxylase) in escherichia coli.the fsba gene controlling the first step of 4-chlorobenzoic acid (4cba) metabolism in the gram-positive soil bacterium arthrobacter globiformis kzt1 has been cloned and analysed in escherichia coli. the e. coli minicells analysis showed that a polypeptide(s) with mr = 58 kda (and/or mr = 32 kda) can be the fcba product(s). despite the gene dose amplification and control of the e. coli inducible plac promoter, the level of functional expression of the fcba gene in e. coli cells seems comparable o ...19911884992
mannopine and mannopinic acid as substrates for arthrobacter sp. strain mba209 and pseudomonas putida na513.the characteristics of mannopine and mannopinic acid utilization by agrobacterium tumefaciens b6s3, arthrobacter sp. strain mba209, and pseudomonas putida na513 were studied. strain b6s3 utilized the four mannityl opines, mannopine, mannopinic acid, agropine, and agropinic acid. it also utilized several mannityl opine analogs, which were modified in either the sugar or the amino acid moiety. it utilized mannopine more rapidly after preincubation on mannopine, mannopinic acid, or glutamine than a ...19911902209
molecular cloning and nucleotide sequencing of the arthrobacter dextranase gene and its expression in escherichia coli and streptococcus sanguis.a bacterial strain, which assimilated dextran and water-insoluble glucan produced by streptococcus mutans, was isolated from soil. the bacterium produced and secreted potent dextranase activity, which was identified as arthrobacter sp. and named cb-8. the dextranase was purified and some enzymatic properties were characterized. the enzyme efficiently decomposed the water-insoluble glucan as well as dextran. a gene library from the bacteria was constructed with escherichia coli, using plasmid puc ...19911859672
the organic functional group in copper-containing amine oxidases. resonance raman spectra are consistent with the presence of topa quinone (6-hydroxydopa quinone) in the active site.resonance raman spectroscopy has been used to probe the structure of the organic cofactor in copper-containing amine oxidases from bovine plasma, porcine kidney, pea seedlings, and the bacterium arthrobacter p1. the enzymes were first derivatized with phenylhydrazine or p-nitrophenylhydrazine; resonance raman spectra were obtained on the intact derivatized enzymes and on a derivatized active-site peptide isolated from bovine plasma amine oxidase. spectra of the intact amine oxidase phenylhydrazo ...19911900285
purification and characterization of haloalcohol dehalogenase from arthrobacter sp. strain enzyme capable of dehalogenating vicinal haloalcohols to their corresponding epoxides was purified from the 3-chloro-1,2-propanediol-utilizing bacterium arthrobacter sp. strain ad2. the inducible haloalcohol dehalogenase converted 1,3-dichloro-2-propanol, 3-chloro-1,2-propanediol, 1-chloro-2-propanol, and their brominated analogs, 2-bromoethanol, as well as chloroacetone and 1,3-dichloroacetone. the enzyme possessed no activity for epichlorohydrin (3-chloro-1,2-epoxypropane) or 2,3-dichloro-1 ...19911846134
a cu(i)-semiquinone state in substrate-reduced amine oxidases.the role of copper in copper-containing amine oxidases has long been a source of debate and uncertainty. numerous electron paramagnetic resonance (epr) experiments, including rapid freeze-quench studies, have failed to detect changes in the copper oxidation state in the presence of substrate amines. one suggestion that copper reduction might occur, has never been confirmed. copper amine oxidases contain another cofactor, recently identified as 6-hydroxydopa quinone (topa quinone), which is reduc ...19911846226
d-xylose (d-glucose) isomerase from arthrobacter strain n.r.r.l. b3728. purification and properties.d-xylose (d-glucose) isomerase was purified to homogeneity in yields of approx. 1 g/kg of wet cells from a strain of arthrobacter that produces it as about 10% of total soluble protein. it is a tetramer of identical 43,114 da subunits containing a preponderance of acidic residues and no cysteine. partial protein sequences were determined as a step to gene cloning. it requires mg2+, co2+ or mn2+ for activity, mg2+ being best; ca2+ is an inhibitor, competitive with mg2+. it is a good d-glucose iso ...19911854338
d-xylose (d-glucose) isomerase from arthrobacter strain n.r.r.l. b3728. gene cloning, sequence and expression.arthrobacter strain n.r.r.l. b3728 superproduces a d-xylose isomerase that is also a useful industrial d-glucose isomerase. the gene (xyla) that encodes it has been cloned by complementing a xyla mutant of the ancestral strain, with the use of a shuttle vector. the 5' region shows strong sequence similarity to escherichia coli consensus promoters and ribosome-binding sequences and allows high levels of expression in e. coli. the coding sequence shows similarity to those for other d-xylose isomer ...19911854339
[quantitative description of microbial growth in a batch culture depending on the physiologic state of inocula].the dynamics of biomass production and the respiration rate of five microorganisms grown as batch cultures were studied in detail. cell suspensions with a known physiological state, i.e. chemostat cultures grown at a particular d value, as well as quasi steady-state populations cultivated with slow feeding and long energy-source starvation were used as inocula. the microorganisms were arbitrary subdivided into two groups. the biomass of pseudomonas fluorescens, bacillus subtilis and debaryomyces ...19911770867
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