matrix-assisted laser desorption ionization (maldi)-time of flight mass spectrometry- and maldi biotyper-based identification of cultured biphenyl-metabolizing bacteria from contaminated horseradish rhizosphere soil.bacteria that are able to utilize biphenyl as a sole source of carbon were extracted and isolated from polychlorinated biphenyl (pcb)-contaminated soil vegetated by horseradish. isolates were identified using matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms). the usage of maldi biotyper for the classification of isolates was evaluated and compared to 16s rrna gene sequence analysis. a wide spectrum of bacteria was isolated, with arthrobacter, serratia, r ...201121821747
antagonistic interaction networks among bacteria from a cold soil environment.microbial antagonism in an arctic soil habitat was demonstrated by assessing the inhibitory interactions between bacterial isolates from the same location. of 139 isolates obtained from five soil samples, 20 antagonists belonging to the genera, arthrobacter, pseudomonas and flavobacterium were identified. inter-genus, inter-species and inter-strain antagonism was observed between the interacting members. the extent of antagonism was temperature dependent. in some cases, antagonism was enhanced a ...201122092175
an isofenphos-methyl hydrolase (imh) capable of hydrolyzing the p-o-z moiety of organophosphorus pesticides containing an aryl or heterocyclic group.organophosphorus pesticide (op) hydrolases play key roles in the degradation and decontamination of agricultural and household ops and in the detoxification of chemical warfare agents. in this study, an isofenphos-methyl hydrolase gene (imh) was cloned from the isocarbophos-degrading strain of arthrobacter sp. scl-2 using the polymerase chain reaction method. isofenphos-methyl hydrolase (imh) showed 98% sequence identity with the isofenphos hydrolase from arthrobacter sp. strain b-5. imh was hig ...201122120622
Baeyer-Villiger monooxygenases in aroma compound synthesis.Baeyer-Villiger monooxygenases (BVMOs) are presented as highly selective and efficient biocatalysts for the synthesis of aroma lactones via kinetic resolution of 2-substituted cycloketones, exemplified with two d-valerolactones, the jasmine lactones and their e-caprolactone homologs. Analytical scale screens of our BVMO library ensued by preparative whole-cell biotransformations led to the identification of two enzymes (cyclohexanone monooxygenase from Arthrobacter BP2 and cyclododecanone monoox ...201121900007
production of xylanase from arthrobacter sp. mtcc 6915 using saw dust as substrate under solid state fermentation.saw dust was used as substrate for xylanase production from arthrobacter sp. mtcc 6915. the study of period of incubation, temperature, ph, carbon, and nitrogen sources for xylanase production was optimized. xylanase production was found to be optimum at an incubation period of 96 hrs (117.0 u/ml), temperature 30°c (105.0 u/ml), and ph 9.0 (102.9 u/ml). the results showed that the xylanase production was found to be higher in the presence of carboxymethylcellulose (176.4 u/ml) and dextrose (126. ...201122013512
calcite biomineralization by bacterial isolates from the recently discovered pristine karstic herrenberg cave.karstic caves represent one of the most important subterranean carbon storages on earth and provide windows into the subsurface. the recent discovery of the herrenberg cave, germany, gave us the opportunity to investigate the diversity and potential role of bacteria in carbonate mineral formation. calcite was the only mineral observed by raman spectroscopy to precipitate as stalactites from seepage water. bacterial cells were found on the surface and interior of stalactites by confocal laser sca ...201122179248
Dynamics and diversity of phosphate mineralizing bacteria in the coral reefs of Gulf of Mannar.Phosphatase Producing Bacteria (PPB) and Inorganic Phosphate Solubilizing Bacteria (IPSB) are important to reef nutrition. These microbes and phosphate concentration play a significant role in the productivity of coral reef ecosystems. A study was conducted in Gulf of Mannar coral reef ecosystem to understand the diversity of these groups of bacteria and their competence in mineralizing the phosphate. The PPB isolates were identified under six genera i.e. Bacillus, Pseudomonas, Micrococcus, Vibr ...201121953521
lc-ms method for screening unknown microbial carotenoids and isoprenoid quinones.the structure of secondary metabolites from microorganisms provides a useful tool for microbial characterization and chemotaxonomic classification. microbial isoprenoid quinones, for example, are well described and used to distinguish among photosynthetic microorganism groups. in addition, isoprenoid quinones can also be found, together with carotenoids, in non-photosynthetic microorganisms. the aim of the present study was to develop a lc-ms/ms method which can analyze and identify these microb ...201222036764
heterologous expression and characterization of two 1-hydroxy-2-naphthoic acid dioxygenases from arthrobacter phenanthrenivorans.a protein fraction exhibiting 1-hydroxy-2-naphthoic acid (1-h2na) dioxygenase activity was purified via ion exchange, hydrophobic interactions, and gel filtration chromatography from arthrobacter phenanthrenivorans sp. nov. strain sphe3 isolated from a greek creosote-oil-polluted site. matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) and tandem ms (ms-ms) analysis revealed that the amino acid sequences of oligopeptides of the major 45-kda protein specie ...201122101055
complete detoxification of tris(1,3-dichloro-2-propyl) phosphate by mixed two bacteria, sphingobium sp. strain tcm1 and arthrobacter sp. strain py1.tris(1,3-dichloro-2-propyl) phosphate (tdcpp), a flame retardant, is regarded as a potentially toxic and persistent environmental contaminant. we previously isolated a tdcpp-degrading bacterium, sphingobium sp. strain tcm1, which, however, produced a toxic metabolite: 1,3-dichloro-2-propanol (1,3-dcp). this study was undertaken to develop a technique for complete tdcpp detoxification using strain tcm1 with a 1,3-dcp-degrading bacterium, arthrobacter sp. strain py1. for efficient detoxification, ...201121956155
degradation of a benzene-toluene mixture by hydrocarbon-adapted bacterial communities.we examined the rate of degradation of a benzene-toluene mixture in aerobic microcosms prepared with samples of an aquifer that lies below a petrochemical plant (siren, uk). five samples exposed to different concentrations of benzene (from 0.6 to 317 mg l(-1)) were used. fast degradation (approx. 1-6 mg l(-1) day(-1)) of both contaminants was observed in all groundwater samples and complete degradation was recorded by the seventh day except for one sample. we also identified the microbial commun ...201121949494
batch biodegradation of para-nitrophenol using arthrobacter chlorophenolicus a6.the present study reports the kinetics of p-nitrophenol (pnp) biodegradation by arthrobacter chlorophenolicus a6 in batch shake flasks for initial pnp concentrations in the range of 25-225 mg l(-1). results of batch growth kinetics of a. chlorophenolicus a6 at various initial pnp concentrations revealed that the culture followed substrate inhibition kinetics with estimated decay coefficient value of 0.0132 h(-1). biokinetic constants involved in the process were estimated by fitting the experime ...201121927857
a novel transaminase, (r)-amine:pyruvate aminotransferase, from arthrobacter sp. knk168 (ferm bp-5228): purification, characterization, and gene cloning.a novel (r)-amine transaminase, which catalyzed (r)-enantioselective transamination of chiral amine, was purified to homogeneity from arthrobacter sp. knk168 (ferm bp-5228). the molecular mass of the enzyme was estimated to be 148 kda by gel filtration and 37 kda by sodium dodecyl sulfate polyacrylamide gel electrophoresis, suggesting a homotetrameric structure. the enzyme catalyzed transamination between amines and pyruvate stereo-specifically. the reaction on 1-methylbenzylamine was (r)-enanti ...201122002066
a novel nadh-dependent and fad-containing hydroxylase is crucial for nicotine degradation by pseudomonas putida.nicotine, the main alkaloid produced by nicotiana tabacum and other solanaceae, is very toxic and may be a leading toxicant causing preventable disease and death, with the rise in global tobacco consumption. several different microbial pathways of nicotine metabolism have been reported: arthrobacter uses the pyridine pathway, and pseudomonas, like mammals, uses the pyrrolidine pathway. we identified and characterized a novel 6-hydroxy-3-succinoyl-pyridine (hsp) hydroxylase (hspb) using enzyme pu ...201121949128
chemoenzymatic synthesis and fcγ receptor binding of homogeneous glycoforms of antibody fc domain. presence of a bisecting sugar moiety enhances the affinity of fc to fcγiiia receptor.structurally well-defined igg-fc glycoforms are highly demanded for understanding the effects of glycosylation on an antibody's effector functions. we report in this paper chemoenzymatic synthesis and fcγ receptor binding of an array of homogeneous igg-fc glycoforms. the chemoenzymatic approach consists of the chemical synthesis of defined n-glycan oxazolines as donor substrates, the expression of the fc domain in a cho cell line in the presence of an α-mannosidase inhibitor kifunensine, and an ...201122004528
characterization of the volatile profile of antarctic bacteria by using solid-phase microextraction-gas chromatography-mass spectrometry.bacteria belonging to the burkholderia cepacia complex (bcc) are significant pathogens in cystic fibrosis (cf) patients and are resistant to a plethora of antibiotics. in this context, microorganisms from antarctica are interesting because they produce antimicrobial compounds inhibiting the growth of other bacteria. this is particularly true for bacteria isolated from antarctic sponges. the aim of this work was to characterize a set of antarctic bacteria for their ability to produce new natural ...201122012672
cloning and extracellular expression of inulin fructotransferase from arthrobacter aurescens sk 8.001 in e. coli.difructose anhydride (dfa) iii is a natural and low-calorie sweetener. it stimulates the absorption of calcium and other minerals. inulin fructotransferase (iftase; ec, catalysing inulin hydrolysis to dfa iii, is considered to be the most promising enzyme for the production of dfa iii.201122081477
removal of hexavalent chromium of contaminated soil by coupling electrokinetic remediation and permeable reactive biobarriers.purpose: in this study, a novel and ecological alternative have been developed to treat soils contaminated with hexavalent chromium coupling two well-known systems: electrokinetic remediation and permeable reactive biobarriers. the electric field promotes the electromigration of the hexavalent chromium oxyanions towards the anode. the biobarriers were placed before the anode electrode, in order to promote the reduction and retention of the chromium migrating in its directio ...201122203400
screening of microorganisms for biodegradation of simazine pollution (obsolete pesticide azotop 50 wp).the capability of environmental microorganisms to biodegrade simazine-an active substance of 2-chloro-s-triazine herbicides (pesticide waste since 2007)-was assessed. an enormous metabolic potential of microorganisms impels to explore the possibilities of using them as an alternative way for thermal and chemical methods of utilization. first, the biotope rich in microorganisms resistant to simazine was examined. only the higher dose of simazine (100 mg/l) had an actual influence on quantity of b ...201121949452
the three-dimensional structure of nylon hydrolase and the mechanism of nylon-6 hydrolysis.we performed x-ray crystallographic analyses of the 6-aminohexanoate oligomer hydrolase (nylc) from agromyces sp. at 2.0 å-resolution. this enzyme is a member of the n-terminal nucleophile (n-tn) hydrolase superfamily that is responsible for the degradation of the nylon-6 industry byproduct. we observed four identical heterodimers (27kda+9kda), which resulted from the autoprocessing of the precursor protein (36kda) and which constitute the doughnut-shaped quaternary structure. the catalytic resi ...201122187439
[High throughput screening atrazine chlorohydrolase mutants with enhanced activity through Haematococcus pluvialis expression system].Developing a high-throughput screening method is of great importance for directed evolution of atrazine chlorohydrolase. A mutagenesis library of atzA from Pseudomonas sp. ADP and Arthrobacter sp. AD1 was constructed using error-prone PCR and DNA shuffling. Candidate mutants were screened through Haematococcus pluvialis expression system, using atrazine as selection pressure. Sequence analysis showed that mutations in the obtained 12 mutants with enhanced activity were all point-substitutions an ...201121847998
Structural insights into the substrate specificity of bacterial copper amine oxidase obtained by using irreversible inhibitors.Copper amine oxidases (CAOs) catalyze the oxidation of various aliphatic amines to the corresponding aldehydes, ammonia and hydrogen peroxide. Although CAOs from various organisms share a highly conserved active-site structure including a protein-derived cofactor, topa quinone (TPQ), their substrate specificities differ considerably. To obtain structural insights into the substrate specificity of a CAO from Arthrobacter globiformis (AGAO), we have determined the X-ray crystal structures of AGAO ...201121984603
Enhancing the recombinant protein expression of halohydrin dehalogenase HheA in Escherichia coli by applying a codon optimization strategy.Halohydrin dehalogenases are attractive biocatalysts in producing a series of important chiral building blocks. Recombinant expression of halohydrin dehalogenase from Arthrobacter sp. AD2 (HheA) in Escherichia coli using T7 promoter-based pGEF(+) system revealed much lower expression level than that of the well-studied halohydrin dehalogenase from Agrobacterium radiobacter AD1 (HheC). In this study, we changed the codon usage in the 5'-end of hheA gene to improve the expression yield of HheA. Ou ...201122112566
construction of escherichia coli-arthrobacter-rhodococcus shuttle vectors based on a cryptic plasmid from arthrobacter rhombi and investigation of their application for functional screening.a cryptic plasmid from arthrobacter rhombi prh1, designated as pprh, was sequenced and characterized. it was 5000 bp in length with a g+c content of 66 mol%. the plasmid pprh was predicted to encode six putative open reading frames (orfs), in which orf2 and orf3 formed the minimal replicon of plasmid pprh and shared 55-61% and 60-69% homology, respectively, with the repa and repb proteins of reported rhodococcal plasmids. sequence analysis revealed a typical cole2-type ori located 45 bp upstream ...201122098420
The overlooked difference between human endogenous and recombinant erythropoietins and its implication for sports drug testing and pharmaceutical drug design.Sequential deglycosylation by exoglycosidase treatment (Reagent Array Analysis Method, RAAM) and subsequent sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) revealed a profound structural difference between human endogenous and recombinant erythropoietins. While both proteins behaved similarly upon digestion with Arthrobacter ureafaciens a-sialidase and Steptococcus pneumoniae ß-D-galactosidase, the action of N-acetyl-ß-D-glucosaminidase from Steptococcus pneumoniae was partl ...201122140023
[study on 2-methylpyridine initial catabolic pathways in arthrobacter sp. km-2mp strain]. 201121861369
impact of pgpr inoculation on growth and antioxidant status of wheat under saline conditions.two plant growth-promoting rhizobacterial (pgpr) strains, bacillus subtilis su47 and arthrobacter sp. su18, were found to tolerate 8% nacl. wheat co-inoculated with these two pgpr strains, and grown under different salinity regimes (2-6 ds m(-1) ), showed an increase in dry biomass, total soluble sugars and proline content. wheat sodium content was reduced under co-inoculated conditions but not after single inoculation with either strain or in the control. the activity of antioxidant enzymes in ...201122136617
cow teat skin, a potential source of diverse microbial populations for cheese production.the diversity of the microbial community on cow teat skin was evaluated using a culture-dependent method based on the use of different dairy-specific media, followed by the identification of isolates by 16s rrna gene sequencing. this was combined with a direct molecular approach by cloning and 16s rrna gene sequencing. this study highlighted the large diversity of the bacterial community that may be found on teat skin, where 79.8% of clones corresponded to various unidentified species as well as ...201222081572
Pseudoclavibacter-like subcutaneous infection: a case report.ABSTRACT:201121933406
the arthrobacter species fb24 arth_1007 (dnab) intein is a arthrobacter species fb24 gene (locus tag arth_1007) was previously annotated as a putative intein-containing dnab helicase of phage origin (arsp-fb24 dnab intein). however, it is not a helicase gene because the sequence similarity is limited to inteins. in fact, the flanking exteins total only 66 amino acids. therefore, the intein should be referred to as the arsp-fb24 arth_1007 intein. the arsp-fb24 arth_1007 intein failed to splice in its native precursor and in a model precursor. we previ ...201122028863
pathway for degradation of 2-chloro-4-nitrophenol in arthrobacter sp. sjcon.degradation of 2-chloro-4-nitrophenol (2c4np) was studied by arthrobacter sp. sjcon, isolated from the soil of a pesticide contaminated site. this strain utilized 2c4np as sole source of carbon and energy and degraded 2c4np with stoichiometric release of nitrite and chloride ions. a metabolite was detected during the study of 2c4np degradation and identified as chlorohydroquinone (chq) by thin layer chromatography (tlc), high performance liquid chromatography (hplc), and gas chromatography-mass ...201121960016
[Isolation and identification of dominant microorganisms in rhizosphere of continuous cropping with peanut].We isolated and identified dominant microorganisms from the rhizosphere of continuous cropping with peanut, to study the relationship between dominant microorganisms and peanut continuous cropping.201121866710
Displaying items 2301 - 2332 of 2332