bacterial xanthine oxidase from arthrobacter s-2.arthrobacter s-2, originally isolated by enrichment on xanthine, produced high levels of xanthine oxidase activity, requiring as little as a 20-fold purification to approach homogeneity with some preparations. molecular oxygen, ferricyanide, and 2,6-dichlorophenol-indophenol served as electron acceptors, but nicotinamide adenine dinucleotide did not. the enzyme was relatively specific when compared with previously studied xanthine-oxidizing enzymes, but at least one purine was observed to be oxi ...1978681279
isolation and characterization of morphogenetic mutants of arthrobacter crystallopoietes.mutants of arthrobacter crystallopoietes that exhibited altered ability to undergo the normal sphere-to-rod-to-sphere morphogenetic cycle were isolated. the procedure used to isolate these mutants involved velocity sedimentation in a sterile sucrose gradient to separate morphogenesis-deficient spherical cells from rod-shaped cells capable of normal morphogenesis. three classes of mutants were obtained: (i) those that cannot form rods, (ii) those that cannot form long rods, and (iii) those that f ...1978681281
growth characteristics of three bacterial isolates from an arctic soil.three bacterial isolates, a pseudomonas sp., a bacillus sp., and an arthrobacter sp., commonly isolated from a hummocky sedge-moss meadow at devon island, n.w.t., canada, were selected for further taxonomic characterization and for a study of the effects of temperature and limiting carbon source on growth. pseudomonas m216 resembled p. putida and bacillus m153, b. carotarum. arthrobacter m51 had growth-factor requirements which were more complex than those of any named species of that genus. the ...1978688099
mechanism of arthrobacter sialophilus neuraminidase: the binding of substrates and transition-state analogs. 1978697876
[effect of exogenous acetyl group acceptors on cholinesterase biosynthesis in arthrobacter simplex cells].the presence of active acetyl or butyryl groups and their acceptors in the growth medium was found to be necessary for the high rate of cholinesterase biosynthesis in the cells of arthrobacter simplex var. cholinesterasus. the active acetyl and butyryl groups are formed upon hydrolysis of acetylcholine and butyrylcholine as well as in the course of glucose metabolism. the following acids were shown to be the acceptors of the acetyl and butyryl groups: butyric, succinic, fumaric, malic acids and, ...1978703648
kinetics of accumulation of a photodynamically induced cell-surface polypeptide in a species of arthrobacter.cells of a species of arthrobacter were incubated in the light with methylene blue, a dye that sensitizes photooxidative reactions by the production of singlet oxygen. an early and major response by the cells to these conditions was stimulation of synthesis of a single cell-surface polypeptide, 21,000 daltons in mass. the rate of synthesis of this polypeptide reached a maximal level about 30 min after the start of illumination. as a consequence, the amount of this polypeptide increased at least ...1978711669
regulation of flavoprotein synthesis studied in vivo in a riboflavin-requiring mutant of arthrobacter oxidans.the biosynthesis of two flavoproteins, 6-hydroxy-d-nicotine oxidase with covalently bound fad and 6-hydroxy-l-nicotine oxidase containing non-covalently bound fad, was studied in wild-type cells and in a riboflavin-requiring mutant of arthrobacter oxidans. in the mutant cells, the rate of synthesis and the maximal activity level of both enzymes after induction by nicotine depended on the amount of added riboflavin. the low rate of synthesis in the presence of 2 micron riboflavin could be enhance ...1978718370
induction and regulation of neuraminidase synthesis in arthrobacter sialophilus.a variety of n-acetylneuraminic acid (acneu) derivatives and analogs were examined as inducers of the extracellular neuraminidase of arthrobacter sialophilus. neuraminidase inductions were primarily studied with tryptone-yeast extract-grown cells after washing and resuspension in a defined replacement medium. the addition of readily metabolizable carbon sources to the latter, such as 0.1% casein hydrolysate, glutamate, or glucose, enhanced enzyme synthesis. enzyme appearance occurred after a lag ...1978721778
fad is covalently attached to peptidyl-trna during cell-free synthesis of 6-hydroxy-d-nicotine oxidase.the process, by which fad is attached covalently to the 6-hydroxy-d-nicotine oxidase apoprotein in d-nicotine-induced cells of arthrobacter oxidans was studied in vitro. [3h]adenine-labelled fad prepared biosynthetically in clostridium kluyveri was incorporated into the 6-hydroxy-d-nicotine oxidase molecule during cell-free translation. fad rather than fmn or riboflavin was thus shown to be the flavin derivative transferred to the polypeptide chain. after short-term protein synthesis on ribosome ...1978738274
effect of temperature, growth rate, and nutrient limitation on the yield and composition of three bacterial isolates from an arctic soil grown in continuous culture. 1978747808
effect of starvation on survival of three bacterial isolates from an arctic soil.three isolates, a pseudomonas sp., a bacillus sp. and an arthrobacter sp., which had been isolated from a meadow soil at devon island, canada, were subjected to starvation under varying conditions. the viabilities of the three isolates during starvation for 30 days in a carbon-free medium was assessed after the organisms had been grown continuously at varying rates in carbon- and nitrogen-limited media at 5 and 15 degrees c. pseudomonas m216 was the most resistant to starvation stress, bacillus ...1978747809
effect of freezing and thawing on survival of three bacterial isolates from an arctic soil.three isolates, a pseudomonas sp., bacillus sp., and arthrobacter sp., which had been isolated from a meadow soil at devon island, canada, were subjected to freezing and thawing at low rates under various conditions. when cells were frozen in sand or soil, survival was dependent on moisture level, storage time, and thaw rate. pseudomonas m216 was most susceptible to freeze-thaw damage under these conditions. arthrobacter m51 was the most resistant of the three isolates when frozen in sand or soi ...1978747810
morphogenetic expression of arthrobacter globiformis 425 in continuous culture with carbon or biotin limitation.when deprived of biotin, arthrobacter globiformis 425 exhibits abnormal morphology (large, branched forms of variable size) and a retardation of its normal growth rate. in chemostat cultures, when cells were grown under glucose limitation, the morphology was normal (coccoids or rods) at specific growth rates between 0.05 and 0.125 h-1 (doubling times between 14 and 5.5 h, respectively) at 25 degrees c. the coccoid-to-rod morphogenesis occurs at a specific growth rate of 0.11 h-1. at the same spe ...1978754873
enzymic assay for lecithin in amniotic fluid.we describe an enzymic method for determining lecithin in amniotic fluid. phospholipase d from savoy cabbage is used to liberate choline from lecithin, and the liberated choline is oxidized to betaine and hydrogen peroxide by choline oxidase from arthrobacter globiformis. the hydrogen peroxide forms a colored complex with phenol and 4-aminoantipyrene in the presence of horseradish peroxidase, and this color is measured spectrophotometrically. the method is relatively easy, precise, and accurate, ...1979761344
[isolation of bacteria assimilating ethanol from natural and industrial substrates]. 1975765694
restriction-enzyme-cleavage maps of bacteriophage m13. existence of an intergenic region on the m13 genome.replicative form dna of bacteriophage m13 was cleaved into specific fragments by an endonuclease isolated from hemophilus aegyptius (endor.haeii) and an endonuclease from arthrobacter luteus (endor.alui). the fragments were ordered as to construct a circular map of the phage m13 genome by: (a) using each fragment as a primer for the synthesis in vitro of its respective neighbour and (b) digesting the isolated fragments with the hemophilus aegyptius enzyme endor.haeii or the hemophilus aphirophil ...1976786638
chondroitinase-producing bacteria in natural habitats.a search was undertaken for bacteria which degrade chondroitin sulfate in nature and to find bacteria with a usefully high rate of chondroitinase (chsase) productivity. first, 253 chsase-producing bacteria were obtained from aquatic and land environments in japan by aerobic and anaerobic screening methods. identification according to bergey's manual of determinative bacteriology or bain and shewan (1968) permitted assignment of the majority of the isolates to seven genera, aeromonas, vibrio, fla ...1975803822
a numerical taxonomic study of coryneform and related bacteria.two hundred and thirty-three strains of coryneform bacteria, including representatives of the genera arthrobacter, brevibacterium, cellulomonas, corynebacterium, erysipelothrix, jensenia, kurthia, listeria, microbacterium, mycobacterium, nocardia and propionibacterium and other related bacteria, were studied using 173 morphological, physiological and biochemical tests. the bacteria were grown on a soil extract medium which allowed growth of all the strains, and all were incubated at 30 degrees c ...1975805825
the fine structure of the branched alpha-d-glucan from the blue-green alga anacystis nidulans: comparison with other bacterial glycogens and phytoglycogen.the fine structure of the glycogen from the blue-green alga anacystis nidulans has been examined. after selective hydrolysis of all (1 yields 6)-alpha-d linkages by a bacterial isoamylase, the resulting mixture of linear chains was subjected to gel permeation chromatography. for purposes of comparison, the glycogens from escherichia coli and arthrobacter sp., amylopectin, phytoglycogen from sweet corn, and shell-fish glycogen were treated similarly. the profiles of the unit chains of a nidulans ...1975806344
microflora and invert sugars in juice from healthy tissue of stored sugarbeets.bacterial populations increased in juice of healthy tissue of sugarbeet roots stored at 5 c. average counts showed a sixfold increase after 150 days of storage. invert sugar levels increased over threefold in "american 4 hybrid a" and remained fairly constant in "mono-hy d-2." the former cultivar also had significantly higher bacterial colony counts than the latter before 90 days of storage. of 36 isolates identified, 16 were pseudomonas spp. including p. chlororaphis; 6 bacillus spp. including ...1975808167
evidence for the occurrence of specific iron (iii)-binding compounds in near-shore marine ecosystems.aqueous extracts of samples from representtive types of near-shore marine ecosystems were examined for growth-promoting activity for the siderochrome auxotroph, arthrobacter jg-9. blue-green algal mat and sea grass samples gave strongly positive responses. relative to desferal (ciba pharmaceutical co., summit, n.j.), concentrations up to 110 ng/g (wet weight) of sample were found. in contrast, extracts of anaerobic muds or macroalgae gave no detectable activity. the hydroxamate type of iron chel ...1975809005
covalently bound fad in d-6-hydroxynicotine oxidase. immunological studies of d- and l-6-hydroxynicotine oxidase: evidence for a d-enzyme precursor.antersera prepared against both enantiozymes, d- and l-6-hydroxynicotine oxidase, formed precipitins in double diffusion tests with their respective antigens only. a mixture of the two antisera caused spur formation of the two precipitin lines obtained with the pure enzymes. antiserum to l-apoprotein reacted with native l-enzyme and l-apoprotein but not with the d-sspecific enzyme. d-6-hydroxynicotine oxidase activity was inhibited by the anti-d-antiserum, leaving the l-enzyme fully active, whil ...1975809438
identification and flagellation of coryneform bacteria from poultry litter.thirth coryneform isolates from poultry litter were identified and checked for motility and flagellation. twenty-seven formed a yellow pigment and 3 were orange. twenty-three yellow strains were found to be arthrobacter citreus, although starch was hydrolyzed by these strains. four strains, including the three mainly pale yellow isolates, grew on citrate plus an ammonium salt and were classified as a. aurescens. three orange strains were found to be brevibacterium linens. only six strains of a. ...1975811345
facultative wood-digesting bacteria from the hind-gut of the termite reticulitermes hesperus.among the facultative bacteria capable of growth on mesquite wood which were isolated from the asceptically dissected hind-gut of the termite reticulitermes hesperus were two strains of bacillus cereus, one strain each of arthrobacter, alcaligenes and serratia, and a very small gram-negative fermentative rod. the b. cereus strains, the serratia marcescens strain and the arthrobacter sp. grew well on a mineral salts alpha-cellusose agar. one of the bacillus cereus strains and serratia marcescens ...1976822127
observations on lysogeny in glutamic acid bacteria.induced lysis occurred in a number of different strains of glutamic acid bacteria. mixed culture experiments indicated that induced cultures produce phages or bacteriocins. temperate phages were isolated from two related strains of brevibacterium divaricatum.1976823863
[mode of action of d-amino acids on the biosynthesis of peptidoglycan (author's transl)].the mechanism of growth inhibition by d-amino acids was studied. d-serine at concentrations from 0.02-0.2 m was sufficient to cause partial growth inhibition in seven species of bacteria representing the four most common types of peptidoglycan. the inhibited cells displayed morphological alterations. in the nucleotide-activated peptidoglycan precursors of these cells, d-alanine residues in position 4 and/or 5 of the peptide moiety were partially or even completely replaced by d-serine. the pepti ...1976825075
thin-layer chromatographic analysis of mycolic acid and other long-chain components in whole-organism methanolysates of coryneform and related taxa.acid methanolysates of strains representing 58 coryneform taxa were examined for mycolic acids and other long-chain constituents by thin-layer chromatography. mycolic esters were detected in the methanolysates of true corynebacteria but not in those from plant pathogenic bacteria, corynebacterium haemolyticum, corynebacterium pyogenes or from representatives of the genera arthrobacter, cellulomonas, curtobacterium, kurthia or oerskovia. thin-layer chromatography of whole-organism methanolysates ...1976825611
adjuvant activity of bacterial glycolipids. 1976825676
microbiological characteristics of natural mineral water.natural, non-carbonated mineral water is, like every other natural water from a spring, never sterile. however, the microbial level is always very low. but after its bottling, the level rises rapidly and numbers of more than 10,000 to 100,000/ml can be reached. in principle 2 groups of bacteria of very different origin and properties can be found in the microbial flora of the bottled, non-carbonated mineral water. allochthonous bacteria will get into the water by contamination from the container ...1976829205
amino acid transport in whole cells and membrane vesicles of arthrobacter pyridinolis. 1977836027
purification and characterization of pyruvate carboxylase from arthrobacter globiformis. 1977851353
effects of temperature on the macromolecular composition and find structure of psychrophilic arthrobacter species.a facultatively psychrophilic bacterium, arthrobacter si 55, grows at 20 degrees c, but growth, as measured by increase of viable cell count, is inhibited at 32 degrees c. corresponding temperatures for an obligate psychrophile, arthrobacter glacialis si 137, are 10 degrees c and 19-20 degrees c. at the higher temperatures for each organism increases of cell mass, as measured by turbidity and of dna, rna, and protein, were not inhibited. at the upper temperatures, fewer septa were formed in arth ...1977856424
distribution of xanthine oxidase and xanthine dehydrogenase specificity types among bacteria.a diverse collection of xanthine-metabolizing bacteria was examined for xanthine-, 1-methylxanthine-, and 3-methylxanthine-oxidizing activity. both particulate and soluble fractions of extracts from aerobically grown gram-negative bacteria exhibited oxidation of all three substrates; however, when facultative gram-negative bacteria were grown anaerobically, low particulate and 3-methylxanthine activities were detected. gram-positive and obligately anaerobic bacteria showed no particulate activit ...1977863854
electron microscopic study of cell surface rings during cell division and morphogenesis of arthrobacter crystallopoietes.the whole cell ultrastructure during cell division and morphogenesis of arthrobacter crystallopoietes was monitored using electron microscopic techniques. glucose-grown spherical cells were inoculated into succinate-based medium. in this medium, the organism undergoes a morphogenetic cycle consisting of elongation of spheres to rods, exponential growth as rods, and fragmentation of rods to spherical cells. raised bands or rings that encircled the cells were evident on the cell surface of both sp ...1977863857
oxidation of linear terpenes and squalene variants by arthrobacter sp.cells of arthrobacter sp. that had been isolated from soil were used to study oxidation of some linear terpenes and squalene variants. the cells oxidized geraniol, nerol, and farnesol to the corresponding aldehydes, with partial conversion of the geometrical isomerism of the alpha,beta-double bond. the squalene variant, squalene-2,3-oxide, was cleaved to 9,10-epoxygeranylacetone and geranylacetone. squalene-2,3-22,23-dioxide was cleaved to 9,10-epoxygeranylacetone. these products were optically ...1977869527
microbiological characteristics of pacific shrimp (pandalus jordani).microorganisms associated with pacific shrimp (pandalus jordani) were isolated and identified. those on the iced raw shrimp, which yielded an average count of 1.6 x 10(6), were predominantly moraxella, pseudomonas, acinetobacter, arthrobacter, and flavobacterium-cytophaga spp. the blanching and peeling reduced the microbial level to 3.3 x 10(4) and also selectively eliminated moraxella spp. the microbial flora changed after each processing sequence, and the heat sensitivity and growth characteri ...1977869532
nitrification in histosols: a potential role for the heterotrophic nitrifier.insufficient populations of nitrosomonas and nitrobacter were found in a pahokee muck soil (lithic medidaprit) to account for the nitrate concentration observed. to determine if heterotrophic nitrifiers could account for some of this discrepancy, a method was developed to measure the levels of heterotrophic nitrifiers in soil. a population of 4.1 x 10(5) arthrobacter per g of dry fallow soil, capable of producing nitrite and/or nitrate from reduced nitrogenous compounds, was observed. amendment ...1977869537
inabilities as an immunoadjuvant of cell walls of the group b peptidoglycan types and those of arthrobacters.the cell walls from several bacterial species whose peptidoglycans are the group b types (schleifer and kandler) and those of two arthrobacters were shown to be inactive or only weakly active as an immunoadjuvant in both induction of delayed-type hypersensitivity and stimulation of circulating antibody levels to ovalbumin when administered to guinea pigs as a water-in-oil emulsion, in sharp contrast to the adjuvant-active cell walls of the group a peptidoglycan types which were previously studie ...1977880204
[acetylcholine metabolism in arthrobacter simplex var. cholinesterasus]. 1977882022
microbiological degradation of bile acids. the preparation of hexahydroindane derivatives as substrates for studying cholic acid degradation.relatively large amounts of 3-(3aalpha-hexahydro-7abeta-methyl-1,5-dioxoindan-4alpha-yl)propionic acid (iib), which is believed to be one of the intermediates involved in the degradation of cholic acid (i), were needed to identify is further degradation products. a simple method for the preparation of this compound was then investigated. arthrobacter simplex could degrade-3-oxoandrost-4-ene-17beta-carboxylic acid (iiia) to 3-(1beta-carboxy-3aalpha-hexahydro-7abeta-methyl-5-oxoindan-4alpha-yl)pro ...1977883962
photodynamic induction of a bacterial cell surface polypeptide.the photodynamic action of several dyes on cells of a bacterium, tentatively identified as a species of arthrobacter, resulted in remarkable stimulation of synthesis of a polypeptide 21,000 daltons in mass. this polypeptide resides on the cell surface and can be solubilized by sodium dodecyl sulfate without lysis of the cells. chlorophyllin and rose bengal are effective in inducing synthesis of the polypeptide in proportion to their ability to sensitize the photooxidation of histidine. etiolated ...1977885841
a new enzymatic method for determination of serum choline-containing phospholipids.a new enzymatic method is presented for the determination of serum choline-containing phospholipids with a combined enzymatic method using phospholipase d (from streptomyces species), choline oxidase (from arthrobacter species) and peroxidase. the method is reproducible, and the results correlate well with those obtained by the conventional digestion method (hoeflmayr, j. and fried, r. (1966) med. ernaehr. 7, 9-10). the method affords better specificity, requires a smaller quantity of the sample ...1977890967
macromolecular synthesis and degradation in arthrobacter during periods of nutrient deprivation.cells of arthrobacter atrocyaneus and a. crystallopoietes, harvested during their exponential phase, were starved in 0.03 m phosphate buffer (ph 7.0) for 28 days. during this time, the cells maintained 90 to 100% viability. experimental results were similar for both organisms. total cellular deoxyribonucleic acid was maintained. measurable degradation rates for deoxyribonucleic acid as determined by radioisotope techniques were not observed, and only during the initial hours of starvation could ...1977914778
action of chondroitinases. iii. ionic strength effects and kinetics in the action of chondroitinase ac. 1977914791
the mechanism of dextransucrase action. activation of dextransucrase from streptococcus mutans omz 176 by dextran and modified dextran and the nonexistence of the primar requirement for the synthesis of dextran. 1977921284
arthrobacter sialophilus sp. nov.; a neuraminidase-producing coryneform.a new species of arthrobacter is described for which the name a. sialophilus is proposed. the organism was obtained by elective culture from soil using collocalia mucoid, and can readily be induced in replacement minimal media with appropriate substrates, to form the enzyme neuraminidase. it undergoes sphere-rod-sphere morphogenesis, is gram-variable, non-motile, aerobic, catalase-positive, oxidase-negative, fails to release acid or gas from saccharides, has a gc content of 56.0 mol % and can gr ...1977922607
natural paucity of anaplerotic enzymes: basis for dependence of arthrobacter pyridinolis on l-malate for growth.previous work has shown that in arthrobacter pyridinolis the transport systems for glucose and several amino acids are respiration coupled, with malate oxidation occurring concomitantly with transport. the requisite malate has to be supplied exogenously, so that growth on glucose or certain amino acids only occurs if malate is also present in the medium. these and other data suggested that a. pyridinolis might be deficient in anaplerotic enzymes, which maintain intracellular levels of dicarboxyl ...1976931946
[oxidation and assimilation pathways of methylated amines in arthrobacter globiformis].the oxidation by arthrobacter globiformis b-175 of tertiary, secondary, and primary amines is catalyzed by specific induced enzymes. trimethylamine is oxidized to dimethylamine via trimethylamine n-oxide by monooxygenase and demethylase. dimethylamine is converted to methylamine by another monooxygenase. methylamine is oxidized to ammonia and formaldehyde by aminoxidase. formaldehyde is assimilated via ribulose monophosphate cycle, which is confirmed by the presence of hexose-phosphate synthase ...1976933867
degradation of alpha-linked d-gluco-oligosaccharides and dextrans by an isomalto-dextranase preparation from arthrobacter globiformis t6. 1976938503
microbiological degradation of bile acids. the preparation of some hypothetical metabolites involved in cholic acid degradation.1. to identify the intermediates involved in the degradation of cholic acid, the further degradation of (4r)-4-[4alpha-(2-carboxyethyl)-3aalpha-hexahydro-7abeta-methyl-5-oxoindan-1beta-yl]valeric acid (iva) by arthrobacter simplex was attempted. the organism could not utilize this acid but some hypothetical intermediate metabolities of compound (iva) were prepared for later use as reference compounds. 2. the nor homologue (iiia) and the dinor homologue (iiib) of compound (iva) were prepared by e ...1976942388
soybean flower-to-seed movement of epiphytic bacteria.epiphytic tracer bacteris (a pseudomonas sp. and an arthrobacter sp.) from soybean buds were introduced into open flowers of greenhouse-grown soybean plants. of the 177 resulting pods cultured after surface disinfection, tracers were recovered from within 24. seed from two of these pods also carried tracers.1976943220
[current achievements in the study of the structure and specificity of flavin enzymes]. 1976944972
nalpha-acetylfusarinines: isolation, characterization, and properties.the isolation, purification, and structural elucidation of a new group of naturally occurring hydroxamic acids are described. these compounds are produced by an unidentified species of penicillium when grown on iron deficient medium. they are closely related structurally to the fusarinines, whose basic structure contains ndelta-(cis-5-hydroxy-3-methylpent-2-enoyl)-ndelta-hydroxy-l-ornithine, but the alpha-amino groups in the new compounds are protected by nalpha acetyl groups. the most complex o ...1976949471
motility as a morphogenic character in the genus arthrobacter.motility in arthrobacter atrocyaneus, a. citreus, and a. simplex was found to correlate with the morphogenic cycle of these organisms. the percentage of the a. atrocyaneus and a. simplex populations that were flagellated at a given time during the growth cycle differed significantly from that of the normorphogenic pseudomonas aeruginosa population. flagellation in a. atrocyaneus was shown to be dependent upon the morphogenic cycle rather than upon growth. the commitment to flagellar synthesis in ...1976956128
ring hydroxylation of p-chlorophenylacetate by an arthrobacter strain.resting cell suspensions of a strain of arthrobacter grown on phenylacetate converted p-chlorophenylacetate to two products. one of the products was identified as 4-chloro-3-hydroxyphenylacetate.1976970937
on the regulation of histidinol dehydrogenase induction in arthrobacter histidinolovorans. 1976971316
[effect of nitrogen source on growth of arthrobacter simplex and its biosynthesis of cholinesterase].the effect of organic and inorganic forms of nitrogen on biomass accumulation and cholinesterase synthesis was studied with arthrobacter simplex var. cholinesterasus. the culture assimilates nitrogen of ammonium compounds better than other forms of inorganic nitrogen; the best nitrogen source for biosynthesis of cholinesterase is ammonium phosphate. nitrogen of nitrates is not assimilated. the amount of biomass is almost twice as high on the medium with peptone, casein or casein hydrolysate as o ...1976979679
the nucleotide sequence of a dna fragment, 71 base pairs in length, near the origin of dna replication of bacteriophage 0x174.part of the nucleotide sequence of a restriction fragment covering the origin of phix174 dna replication 1 has been determined. the fragment a7c was obtained by digestion of phix174 rf dna by the restriction enzyme from arthrobacter luteus, alu 1. it was further cleaved into two fragments, one large and one small, by the action of the restriction enzyme from haemophilus aegyptius, hae 111. the nucleotide sequence of the small fragment has been determined by analysis of the transcription products ...1976995652
[degradation of (-)-ephedrine by arthrobacter globiformis (author's transl)].a bacterium which grows on and completely degrades ephedrine as the only source of carbon was isolated from soil. this bacterium was identified as arthrobacter globiformis. from the culture medium the following metabolites were isolated: 1-hydroxy-1-phenyl acetone, benzoic acid, pyrocatechol, cis-cis-muconic acid and methylamine. the mechanism of metabolism is discussed and compared with that in mammals.1976998048
4-keto-gamma-carotene(beta, psi-caroten-4-one), the major pigment of an arthrobacter sp. 19761000359
[effect of various carbon sources on cholinesterase formation by arthrobacter simplex var. cholinesterasus].the effect of carbohydrates, aromatic alcohols, choline and acetylcholine on the biomass production and biosynthesis of choline esterase was studied with arthrobacter simplex var. cholinesterasus. fructose was found to be the best carbon source for the biomass accumulation and synthesis of choline esterase. almost the same amount of the enzyme was produced on media with glucose and maltose as on the medium with fructose though the biomass yield was much lower. on the contrary, the biomass produc ...19761004262
[survival of epiphytic microorganisms following sublimation dehydration and storage].epiphytic microorganisms belonging to the genera pseudomonas, agrobacterium, achromobacter, flavobacterium, mycobacterium, mycococcus, nocardia, corynebacterium, arthrobacter, bacillus, and microbacterium were dehydrated by sublimation employing two suspension media, and were stored at 3-5 degrees c during 1-2.5 years. survival of epiphytic microorganisms upon dehydration by sublimation and storage was found to depend on their taxonomy (genus, species, strain) and the composition of the suspensi ...19761004279
conversion of (+/-)-synephrine into p-hydroxyphenylacetaldehyde by arthrobacter synephrinum. a novel enzymic reaction.a partically purified enzyme from arthrobacter synephrinum was found to catalyse the conversion of (+/-)-synphrine into p-hydroxyphrenylacetaldehyde and methylamine. the enzyme is highly specific for synephrine and is distinctly different from monoamine oxidase.19761008837
growth and pigment production by arthrobacter pyridinolis n. sp.a new bacterium capable of growing on 2-hydroxypyridine as sole source of carbon and nitrogen was isolated from soil. during its growth on solid medium, approximately 50% of this substrate was converted to a brilliant blue crystalline pigment which was deposited extracellularly in the colony mass. the pigment was identical to that produced by arthrobacter crystallopoietes during its growth on 2-hydroxypyridine. the new isolate exhibited the typical cycle of morphogenesis characteristic of the ge ...19761015948
[degradation of 4-chlorobenzoic acid by an arthrobacter species (author's transl)].an arthrobacter sp. growing on 4-chlorobenzoic acid as its sole source of carbon excretes 4-hydroxygenzoic acid and protocatechuic acid into the culture medium. protocatechuic acid is further attacked by "meta"-cleavage. during growth of the arthrobacter sp. on benzoic acid cis-cis muconic acid can be isolated from the medium, suggesting the involvement of the "ortho"-cleavage pathway. the enzymes both for the "meta"- and the "ortho"-cleavage pathway are inducible.19761015950
induction of d-6-hydroxynicotine oxidase in resting cells of arthrobacter oxidans.resting cell suspensions of arthrobacter oxidans were shown to synthesize the inducible enantiozyme, d-6-hydroxynicotine oxidase, in the presence of d-nicotine or d-6-hydroxynicotine. the corresponding l-enantiomers, as well as gamma-methylaminopropyl-(6-oh-pyridyl-3)-ketone, which is the product of the reaction catalyzed by the enzyme, were ineffective as inducers. l-6-hydroxynicotine inhibited induction by d-nicotine and d-6-hydroxynicotine while l-nicotine inhibited induction by d-6-hydroxyni ...19761015955
macromolecular synthesis and cell division during morphogenesis of arthrobacter crystallopoietes.the sphere-rod-sphere morphology cycle of arthrobacter crystallopoietes was accompanied by changes in the rate of growth and the rates of dna, rna and protein synthesis. the patterns of macromolecule synthesis resembled those found in other bacteria during a step-up followed by a step-down in growth rate. during the step-up in growth spherical cells grew into rods and macromolecules were synthesized in the absence of cell division. during step-down, successive rounds of septation produced progre ...19761015960
microbiological degradation of bile acids. the conjugation of a certain cholic acid metabolite with amino acids in corynebacterium equi.1. (4r)-4[4alpha-(2-carboxyethyl)-3aalpha-hexahydro-7abeta-methyl-5-oxoindan-1beta-yl]valeric acid (ii) could not be utilized by arthrobacter simplex, even though the acid was one of the metabolites formed from cholic acid (i) by this organism. therefore the further degradation of the acid (ii) by corynebacterium equi was investigated to identify the intermediates involved in the cholic acid degradation. 2. the organism, cultured in a medium containing the acid (ii) as the sole source of carbon, ...19761016254
[preliminary observations on the characterization of a microbial strain isolated from mud and active in saline medium with added phenanthrene]. 19761016622
action of chondroitinases. ii. numerical calculation of the degree of multiple attack.further investigation was carried out on the action patterns of two chondroitinase-ac [ec] preparations obtained from arthrobacter aurescens and flavobacterium heparinum. to infer the action patterns of the chondroitinases, we proposed a new method for the calculation of the degree of multiple attack, based on the concept established by robyt and french ((1967) arch. biochem. biophys. 122, 8-16). it was shown that the degree of multiple attack (dm) is represented by the ratio of the ini ...19761018015
identification of the cultivable bacteria in dental plaque from the beagle dog.fifteen different genera of microorganisms were isolated from the 27-month-old plaque of two beagle dogs. they were as follows: pseudomonas, proteus, neisseria, escherichia, staphylococcus, streptococcus, lactobacillus, nocardia, actinomyces, corynebacterium, micrococcus, microbacterium, brevibacterium, arthrobacter, and kurthia. the dog plaque was characterized by a low number of streptococci and the presence of several noncarbohydrate-fermenting organisms.19761069758
arthrobacter luteus restriction endonuclease cleavage map of phi chi 174 rf dna. 19751081295
improvements of the membrane filter method for dna:rrna hybridization.we describe and recommend the following improvements of dna:rrna membrane filter hybridization methods. one of our aims was to avoid dna release from filter discs during hybridization. 1. our hybridization conditions are 2 ssc in aq. dest., with 20% formamide, 50 c, overnight for 16 hr. 2. duplexing is over in 8-10 hr. 3. formamide has to be very pure (o.d. less than or equal to 0.2/cm light path at 270 nm). 4. rnaase treatment: 250 mug/5 ml 2 ssc/filter at 37 c for 1 hr. 5. our conditions for s ...19751082296
the genetic map of bacteriophage phix174 constructed with restriction enzyme fragments. 19761084616
cleavage map of bacteriophage phix174 rf dna by restriction enzymes.phix rf dna was cleaved by restriction enzymes from haemophilus influenzae rf (hinf i) and haemophilus haemolyticus (hha. i). twenty one fragments of approximately 25 to 730 base pairs were produced by hinf i and seventeen fragments of approximately 40 to 1560 base pairs by hha i. the order of these fragments has been established by digestion on haemophilus awgyptius (hae iii) and arthrobacter luteus (alu i) endonuclease fragments of phix rf with hinf i and hha1. by this method of reciprocal dig ...19761085927
sequential growth of bacteria on crude modification of the enrichment culture procedure three bacterial strains capable of degrading crude oil in sea water were isolated in pure culture, up-2, up-3, and up-4. strain up-2 appears to be highly specialized for growth on crude oil in sea water since it showed strong preference for oil or oil degradation products as substrates for growth, converted 66% of the oil into a form no longer extractable by organic solvents, quantitatively degraded the paraffinic fraction (gas chromatographic ...19751096820
microbiological characteristics of dungeness crab (cancer magister).aerobic, heterotropic microorganisms of dungeness crab (cancer magister) were isolated from raw crab, cooked crab, crab meats obtained during commercial processing, and from retail crab meat samples. each microbial isolate was then identified to the genus level employing the revised replica plating procedure. microbial groups most commonly isolated from crab meat were, in the order of predominance, moraxella, pseudomonas, acinetobacter, arthrobacter, micrococcus, flavobacterium-cytophaga, and ba ...19751096824
oxidative degradation of squalene by arthrobacter organism isolated from soil and identified as arthrobacter sp. was studied for its squalene degradation. the degradation product from squalene, which accumulated in the culture broth, was isolated and identified as trans-geranylacetone by mass spectrometry, gas chromatography, infrared spectrometry, and nuclear magnetic resonance spectrometry. addition of a high concentration of k2hpo4 to the culture medium resulted in accumulation of fairly large amounts of carboxylic acids in addition to ge ...19751115507
indicator media for microorganisms degrading chlorinated pesticides.a bromocresol purple liquid indicator medium and an eosin-methylene blue agar have been developed for the demonstration and isolation of microorganisms able to degrade the chlorinated herbicide, 2,4-dichlorophenoxyacetic acid (2,4-d). plates of the eosin-methylene blue agar indicate individual 2,4-d-degrading bacterial colonies. both indicator systems show the production of acid, presumably hydrochloric, during degradation of the 2,4-d in the media. concentrations of 2,4-d required to give an ac ...19751116035
a nutritional and taxonomic survey of arthrobacter soil hundred and sixty arthrobacter soil isolates, four groups of 40 from each of four soil types, and 17 arthrobacter named strains were characterized on the basis of 203 different nutritional tests performed on each culture. one hundred and twenty-one compounds were examined as sole sources of carbon, 44 compounds as sole sources of nitrogen, 22 carbohydrates for the production of acid, and 16 tests were done for tolerance to various dyes. the four groups of soil arthrobacters differed markedly ...19751116046
[crystal-transformation of hydrocortisone effected by a culture of mycobacterium globiforme 193]. 19751117087
the microbial metabolism of di-n-butyl phthalate and related dialkyl phthalates. 19751125463
differentiation of arthrobacter soil isolates and named strains from other bacteria by reations on dye-containing media.twenty-five gram-negative and 62 gram-positive bacterial cultures, of which 37 werearthrobacters, were tested, using a multipoint inoculation device, for responses on varying concentrations of 35 dyes. both selective (growth vs. no growth) and differential (dye absorption) responses were obtained. it was possible to differentiate the arthrobacters from the other gram-positive bacteria tested as well as to distinguish separately each arthrobacter-named strain. the results indicated that dyes have ...19751125859
action of arthrobacter ureafaciens inulinase ii on several oligofructans and bacterial levans.1. arthrobacter ureafaciens inulinase ii which converts inulin to di-d-fructofuranose 1,2' : 2,3' dianhydride (difructose anhydride iii) leaving a small amount of oligosaccharides, was investigated in order to characterize its mode of action. 2. after the enzymatic reaction on the glucose-terminated inulin molecules had been completed, the oligosaccharides left in the enzyme digest were isolated, and identified to be the fructose-glucose oligosaccharides; o-beta-d-fructofuranosyl-(2 leads to 1)- ...19751148257
microbial metabolism of phenolic amines: degradation of dl-synephrine by an unidentified arthrobacter.microorganisms capable of degrading dl-synephrine were isolated from soil of citrus gardens by enrichment culture, with dl-synephrine as the sole source of carbon and nitrogen. an organism which appears to be an arthrobacter, but which cannot be identified with any of the presently recognized species was predominant in these isolates. it was found to metabolize synephrine by a pathway involving p-hydroxyphenylacetaldehyde, p-hydroxyphenylacetic acid, and 3,4-dihydroxyphenylacetic acid as interme ...19751150621
metabolism of dichlobenil by microorganisms in the aquatic environment. 19751150978
microbial cleavage of the tomatidine spiroketal sidechain. 19751177446
[arthrobacter siderocapsulatus isolated from lake water].two microbial strains have been isolated from lake water. the strains oxidize ferrous compounds and manganese. by the structure of microcolonies and the character of deposited oxides of these metals, the strains are identical to the genus siderocapsa. however, according to their growth cycle and some morpho-physiological characteristics, they were included into the genus arthrobacter (corynebacteriaceae). since these microorganisms differ, by their cultural and morpho-physiological properties, f ...19751177780
stereochemistry of delta 1,4 unsaturation in microbial transformation of cholesterol. 19751182921
induction of morphogenesis in the genus arthrobacter.thirty-eight different compounds were used with 17 species of arthrobacter to determine their ability to support growth and to induce the morphogenic cycle. in most cases, when a compound supported growth, it also induced the rod phase of growth. however, in a few cases, a compound would support growth with cells remaining in the coccoidal phase throughout the growth cycle. arthrobacter crystallopoietes was unique in the most compounds that supported growth did not induce the rod phase of the mo ...19751184572
[modifications of the antigenic composition of arthrobacter simplex atcc 6946 in the presence of reichstein's substance s]. 19751186519
[formation of primary alcohols and palmitic acid in the microbiological oxidation of hexadecane].forty seven microbial strains oxidized hexadecane to form primary cetol and palmitic acid. the maximum quantitiy of the compounds was accumulated in the exponential phase of growing cultures (for 72 hours) and for 6 to 16 hours of incubation of resting cell suspensions. candida yeast were shown by the gas-liquid chromatography method to be the most active producers of cetol.19751187568
[on the taxonomy and physiology of bacteria utilizing hydrocarbons in the sea (author's transl)].using mineral media with gas oil as sole carbon source, 191 bacterial strains were isolated from the costal area of marseille. these strains were attributed to achromobacter, alcaligenes, pseudomonas, acinetobacter and arthrobacter genera. amongst isolated strains there was a predominance of the alcaligenes-achromobacter group over others genera. growth and respiratory activity of 5 strains were studied on hexadecane and acetate. respiratory activity on hexadecane of 32 strains cultured on aceta ...19751190640
peptidoglycan compositions of a new strain of arthrobacter crystallopietes during sphere-rod morphogenesis.arthrobacter crystallopoieties atcc 15481 was used to isolate a new strain. designated arthrobacter crystallopoieties epsr-16, which had a mass doubling time in brain heart infusion broth and in glucose/salts/yeast extract medium of 30 min compared to 2.40 h for the parent strain in similar media. the growth rates for the new strain and for the parent were close to 12 h in glucose/salts medium. the new strain formed well-separated cocci and diplococci in glucose/salts medium, and upon nutrient s ...19751201285
the microbial degradation of cyclohexane carboxylic acid. 19751202160
[microbiological transformation and degradation of pesticides]. 19751206144
bacterial carotenoids, xlvi. c50-carotenoids, 14. c50-carotenoids from arthrobacter glacialis.from the psychrophilic bacterium arthrobacter glacialis have been isolated three c50-carotenoids with molecular formulae c50h72o2: the bicyclic decaprenoxanthin (1a, 7% of total carotenoids), the aliphatic bisanhydrobacterioruberin (2a, 10%) and the monocyclic a.g. 470 (3a, 83%). decaprenoxanthin (1) and bisanhydrobacterioruberin (2) were in all respects, including chiroptical properties, identical with known carotenoids. the constitution of the previously undescribed a.g. 470 (3a) followed from ...19751211003
[spectrophotometric characteristics of the coccoid forms of actinomycetes].ir spectra of thw whole cells of the coccoid forms (mycococcus and micrococcus) isolated from lithophilous lichen were compared with ir spectra of the collection cultures of micrococcus and arthrobacter. generic spectral characteristics of mycococcus and micrococcus are presented. spectral heterogeneity within the genus arthrobacter complicates the diagnosis. the cultures of the mycococcus genus were divided into three groups according to their spectral characteristics. spectral scans of the stu ...19751214609
[facultative methylotroph belonging to the genus arthrobacter].a bacterial strain has been isolated and identified, on the basis of its morphological and physiologo-biochemical properties, as arthrobacter globiformis. the bacterium is a facultative methylotroph and grows not only on media with various organic compounds but also in the presence of methylated amines as a sole source of carbon, nitrogen, and energy. other c1-substrates were not utilized.19751214618
microbial decomposition of 2-ethylpyridine, 2,4-lutidine & 2,4,6-collidine. 19751218933
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